KR20150046535A - Composition for Treating and Preventing DNA Damage by Nanosize TiO₂Toxin - Google Patents
Composition for Treating and Preventing DNA Damage by Nanosize TiO₂Toxin Download PDFInfo
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Abstract
Description
본 발명은 Nanosize TiO₂독성에 의한 DNA 손상 예방 및 치료용 조성물에 관한 것으로, 보다 상세하게는 작은 나노사이즈의 TiO₂에 의한 임파구 DNA의 손상 및 기타 독성을 억제하는 조성물로서, 천연 파이토케미컬 또는 항산화제를 포함하는 Nanosize TiO₂독성에 의한 DNA 손상 예방 및 치료용 조성물에 관한 것이다. The present invention relates to a composition for preventing and treating DNA damage caused by nanosize TiO 2 toxicity, and more particularly, to a composition for inhibiting DNA damage and other toxicity caused by small nano-sized TiO 2, which comprises a natural phytochemical or antioxidant To a composition for preventing and treating DNA damage by Nanosize TiO 2 toxicity.
현재 많은 나라에서 사용이 인정되고 있는 TiO₂는 비타르계 색소로 세계적으로 연간 약 300만 톤이 생산되고 있다. Microsize의 TiO₂는 산화력이 매우 커서 항균 작용이 크고 악취제거 및 살균작용이 있다. 또한 매우 안정하며 생물학적으로 반응을 하지 않아 환경 및 인체에 무해하다. 이에 따라 자외선 차단제 및 화장품뿐만 아니라 일상생활에서 쉽게 접하는 가정용품과 플라스틱 제품, 의료용품, 태양전지, 임플란트, 흰색 페인트 등으로 사용되고 있다. Currently, TiO2, which is recognized as being used in many countries, is a non-tar pigment, which produces about 3 million tons per year worldwide. TiO2 of microsize is highly oxidative and has a great antibacterial effect, has odor removal and sterilizing action. It is also very stable and biologically unresponsive, harmless to the environment and human body. Accordingly, it is used not only in sunscreen agents and cosmetics, but also in housewares and plastic products, medical supplies, solar cells, implants, and white paints, which are easily accessible in everyday life.
이와 같이 Microsize 이상의 TiO₂는 안정적이지만 Nanosize의 TiO₂는 체내에서 hydrogen peroxide와 nitric oxide 생산을 증가시켜, 세포나 조직에 손상을 불러 올 수 있고 만성 염증을 발생시킨다. 따라서 TiO₂입자크기가 작아짐에 따라 유발되는 문제들의 위험성에 대한 연구가 필요하다. 이 나노TiO₂입자의 독성을 Comet assay 실험을 통해 DNA 손상을 확인하고 이에 따른 위험성을 완화하는 천연 조성물의 개발이 필요하게 된다. Thus, while TiO2 above microsize is stable, Nanosize's TiO2 increases the production of hydrogen peroxide and nitric oxide in the body, causing damage to cells and tissues, and causing chronic inflammation. Therefore, it is necessary to study the risk of the problems caused by the smaller TiO2 particle size. To evaluate the toxicity of the nano TiO 2 particles through Comet assay, it is necessary to develop a natural composition to identify DNA damage and mitigate the risk.
Comet assay는 유전자 손상 정량분석법이라고 하는데, DNA가 손상되어 생기는 조각들로 인해 나타나는 혜성 모양에서 이름을 따왔으며, DNA 조각의 이동거리를 확인하여 손상 정도를 파악하게 된다. 이 실험법은 민감하게 생물 시료 분석이 가능하며, 유해물질을 처리한 생물 조직에서 추출한 세포에도 적용 가능하여 유전독성 및 바이오 모니터링 분야에서 점차 중요한 기술로 인식되고 있다. The Comet assay is called genetic damage assay, which is named after a comet that appears due to fragments of DNA damage. This method can be applied to cells extracted from biological tissues treated with harmful substances, and it is recognized as an increasingly important technology in the fields of genotoxicity and bio-monitoring.
본 발명에서는 나노TiO₂입자가 쓰일 수 있는 제품들에 대한 임파구 DNA 손상을 항산화제를 통해 저감할 수 있음을 증명해내고자 하였다. 실험 내용으로 나노TiO₂입자에 대한 comet assay를 통해 임파구 DNA 손상을 입히는 유의적인 농도범위를 찾고, 나노TiO₂입자의 유의적 DNA손상 조건에서 다양한 항산화 물질을 처리하여 DNA손상을 줄이는 결과를 얻었다. In the present invention, it has been attempted to demonstrate that lymphocyte DNA damage to nanoparticle TiO2 particles can be reduced through antioxidants. The results of comet assay for nano TiO2 particles showed significant concentration range of lymphocyte DNA damage and the effect of various antioxidants on the DNA damage condition of nano TiO2 particles resulted in reduction of DNA damage.
상기와 같은 문제점을 해결하기 위하여, 나노 TiO₂입자의 DNA 손상을 억제 내지 감소를 위해 천연 파이토케미컬 또는 항산화제를 포함하는 Nanosize TiO₂독성에 의한 DNA 손상 예방 및 치료용 조성물을 제공하는 것을 목적으로 한다. In order to solve the above problems, it is an object of the present invention to provide a composition for preventing and treating DNA damage by nanosize TiO 2 toxicity including natural phytochemical or antioxidant for inhibiting or reducing DNA damage of nano TiO 2 particles.
상기와 같은 목적을 달성하기 위해 본 발명은 천연 파이토케미컬을 포함하는 Nanosize TiO₂독성에 의한 DNA 손상 예방 및 치료용 조성물을 제공한다. In order to accomplish the above object, the present invention provides a composition for preventing and treating DNA damage by nanosize TiO 2 toxicity including natural phytochemicals.
또한, 본 발명에서 상기 천연 파이토케미컬은 레스베라트롤(resveratrol) 또는 설포라판(Sulforaphane) 또는 커큐민(Curcumin) 중 어느 하나 이상을 포함하는 것을 특징으로 한다. In the present invention, the natural phytochemical is characterized by containing at least one of resveratrol, sulforaphane or curcumin.
또한, 본 발명은 바람직한 일 실시예에 따라, 항산화제를 더 포함할 수 있다. 바람직하게는, 상기 항산화제는 비타민 C 또는 N-acetylcysteine(NAC) 일 수 있다. In addition, the present invention may further comprise an antioxidant, according to a preferred embodiment. Preferably, the antioxidant may be vitamin C or N-acetylcysteine (NAC).
또한, 본 발명은 항산화제를 포함하는 Nanosize TiO₂독성에 의한 DNA 손상 예방 및 치료용 조성물을 제공한다. The present invention also provides a composition for preventing and treating DNA damage by Nanosize TiO 2 toxicity comprising an antioxidant.
본 발명에 따른 Nanosize TiO₂독성에 의한 DNA 손상 예방 및 치료용 조성물은 비타민 C 와 NAC 항산화제뿐만 아니라 resveratrol, Sulforaphane, Curcumin등의 파이토케미컬이 TiO₂ 나노입자에 의한 임파구 DNA 손상을 억제하는데 효과적이다. The composition for prevention and treatment of DNA damage by Nanosize TiO 2 toxicity according to the present invention is effective for inhibiting lymphocyte DNA damage by TiO 2 nanoparticles such as resveratrol, sulforaphane and curcumin as well as vitamin C and NAC antioxidants.
도 1은 나노 TiO₂입자의 농도증가에 따른 임파구 DNA 손상을 나타낸 것이다.
도 2는 나노 TiO₂80ug/ml에 의한 임파구 DNA손상에 대한 NAC의 억제효능을 나타낸 것이다.
도 3은 나노 TiO₂80ug/ml에 의한 임파구 DNA손상에 대한 Vit C의 억제효능을 나타낸 것이다.
도 4는 나노 TiO₂80ug/ml에 의한 임파구 DNA손상에 대한 Resveratol의 억제 효능평가를 나타낸 것이다.
도 5는 나노 TiO₂80ug/ml에 의한 임파구 DNA손상에 대한 Sulforaphane의 억제효능 평가를 나타낸 것이다.
도 6은 나노 TiO₂80ug/ml에 의한 임파구 DNA손상에 대한 Curcumin의 억제효능 평가를 나타낸 것이다. Figure 1 shows lymphocyte DNA damage as the concentration of nano TiO2 particles increases.
Fig. 2 shows the inhibitory effect of NAC on lymphocyte DNA damage caused by nano-TiO2 of 80 ug / ml.
Fig. 3 shows the inhibitory effect of vitamin C on lymphocyte DNA damage caused by nano-TiO2 at 80ug / ml.
FIG. 4 shows an evaluation of the inhibitory effect of Resveratol on lymphocyte DNA damage caused by nano-TiO 2 of 80 ug / ml.
FIG. 5 shows the inhibitory effect of sulfaphene on lymphocyte DNA damage caused by nano-TiO2 at 80ug / ml.
Fig. 6 shows the inhibitory effect of curcumin on lymphocyte DNA damage caused by nano-TiO2 at 80ug / ml.
본 발명은 Nanosize TiO₂독성에 의한 DNA 손상 예방 및 치료용 조성물에 관한 것으로, 보다 상세하게는 작은 나노사이즈의 TiO₂에 의한 임파구 DNA의 손상 및 기타 독성을 억제하는 조성물로서, 천연 파이토케미컬 또는 항산화제를 포함하는 Nanosize TiO₂독성에 의한 DNA 손상 예방 및 치료용 조성물에 관한 것이다. The present invention relates to a composition for preventing and treating DNA damage caused by nanosize TiO 2 toxicity, and more particularly, to a composition for inhibiting DNA damage and other toxicity caused by small nano-sized TiO 2, which comprises a natural phytochemical or antioxidant To a composition for preventing and treating DNA damage by Nanosize TiO 2 toxicity.
이하, 실시예를 들어 본 발명을 자세히 설명하고자 한다.
Hereinafter, the present invention will be described in detail with reference to Examples.
< < 실시예Example 1: 나노 1: Nano TiOTiO ₂입자 준비>₂ particle preparation>
Sigma-Aldrich에서 구입한 Titanium(IV) oxide (anatase, nanopowder, <25 nm particle size, 99.7% trace metals basis, 637254) 10mg을 1X PBS 1mL에 녹여 voltexing 후, sonication 30min하여 나노TiO₂ stock solution을 제조하였다. 그 후, 1X PBS 900mL에 나노TiO₂ stock solution 100mL을 넣어 dilution하였다.
10 mg of Titanium (IV) oxide (anatase, nanopowder, <25 nm particle size, 99.7% trace metals basis, 637254) purchased from Sigma-Aldrich was dissolved in 1 mL of 1X PBS and subjected to voltexing and sonication for 30 min to prepare a nano TiO 2 stock solution . Then, 900 mL of 1X PBS was diluted with 100 mL of nano-TiO 2 stock solution.
< < 실시예Example 2 : 2 : CometComet assayassay > >
6~7주령의 mouse에서 blood를 분리한다. 각각의 E-tube에 1X PBS 800mL와 Histopaque 200mL을 넣는다. blood는 Histopaque과 같은 비율이 되도록 200mL을 취해 PBS가 들어있는 e-tube에 넣고 잘 섞은 후 Histopaque이 들어있는 e-tube에 옮겨 넣는다. 이 때, e-tube를 90˚로 기울여서 천천히 넣고 Blood와 Histopaque이 섞이지 않도록 한다. Centrifuge (1450rpm, 5mic, 20℃) 후, 백혈구와 임파구가 포함된 Buffy coat층만 분리하여 PBS 1mL에 넣는다. 다시 Centrifuge (1450rpm, 5mic, 20˚C) 후, 상등액을 제거한다. Separate blood from 6 to 7 week old mice. Add 800 mL of 1X PBS and 200 mL of Histopaque to each E-tube. Take 200 mL of blood to the same ratio as the Histopaque, place it in an e-tube containing PBS, mix well and transfer it to an e-tube containing Histopaque. At this time, tilt the e-tube at 90 ° and slowly insert it so that the blood and the histopaque do not mix. After centrifugation (1450 rpm, 5 mic, 20 ° C), only the buffy coat layer containing white blood cells and lymphocytes is separated and placed in 1 mL of PBS. After centrifuging again (1450 rpm, 5mic, 20 ° C), the supernatant is removed.
실험 30분 전 미리 sonication한 TiO₂를 농도별로 e-tube에 만든 후 vortexing하고 앞의 과정에서 만들어진 pellet이 있는 e-tube와 항산화제를 이용하여 전처리과정을 마친 pellet이 있는 e-tube에 넣는다. 이 때, tip으로 pellet을 떼어주면서 깨끗하게 분리되도록 한다. Ice에 5분간 방치한 후, Centrifuge (1450rpm, 5mic, 20˚C)하여 상등액 제거한다. 그 후, PBS 1ml로 washing하고 다시 Centrifuge (1450rpm, 5mic, 20˚C) 후, 0.7% LMA 100mL를 넣어 pellet을 녹인다. 1% NMA을 묻힌 slide에 duplicate를 만든 후, Cover glass(25x25)로 덮어 철판 위에 올려둔다. 4℃ 냉장고에서 30 min 보관해두었다가 Cover glass를 수평으로 밀어주면서 제거한다. 그 위에 다시 agarose 100mL를 뿌려 cover glass(25x50)으로 덮는다. 4℃ 냉장고에서 30 min 보관해두었다가 Cover glass를 제거하고 Lysis buffer(Lysis buffer 89% + DMSO 10% + Triton X-100 1ml)가 들어있는 Jar에 slide를 넣고 호일로 감싼 후 4˚C에서 1hr동안 반응시킨다. Thirty minutes before the experiment, sonicate TiO2 in e-tube for each concentration, vortexing and put in the e-tube with pellet with pre-treatment using e-tube and antioxidant with pellet made in the previous process. At this time, detach the pellet with a tip and remove it cleanly. Leave in ice for 5 minutes, then remove the supernatant by Centrifuge (1450 rpm, 5mic, 20˚C). After that, wash with 1 ml of PBS, centrifuge again (1450 rpm, 5 mic, 20 ° C) and dissolve the pellet with 100 ml of 0.7% LMA. Make a duplicate on the slides embedded with 1% NMA, cover with 25x25 cover glass and place on a steel plate. Store in the refrigerator for 30 minutes at 4 ° C, then remove the cover glass while pushing it horizontally. Spread 100 mL of agarose over it and cover with cover glass (25x50). After removing the cover glass at 4 ° C for 30 min, the cover glass was removed. The slide was put in a jar containing lysis buffer (Lysis buffer 89% +
암실에서 Electrophoresis 틀의 가운데에 slide를 차례로 올려준 다음 buffer(10N NaOH 30ml + 200mM Na₂EDTA 5ml, total DDW 1L)를 부어준다. 그리고 20분동안 unwinding시킨 후, 300mA/25V에서 20min동안 전기영동한다. 전기영동이 끝나면 Tris buffer(Tris 48.5g + DDW 800mL (pH 7.5))로 washing (5min, 3번)하고 100% EtOH에 slide를 5min 간 탈수시킨 후, Slide를 세워서 10~15min dry한다. 이 slide를 4℃ 에서 하루 보관하여 관찰 전에 EtBr을 넣고 염색 후 현미경과 KOMET프로그램으로 관찰한다.
In the dark room, slide the slide in the center of the electrophoresis frame, then pour buffer (10ml NaOH 30ml + 200ml Na2EDTA 5ml, total DDW 1L). After 20 min of unwinding, electrophoresis is carried out at 300 mA / 25 V for 20 min. When electrophoresis is complete, wash (5 min, 3 times) with Tris buffer (Tris 48.5 g + DDW 800 mL, pH 7.5) and dehydrate the slides in 100% EtOH for 5 min. The slides are stored at 4 ° C for one day. Before observation, EtBr is added to the slides and observed with a microscope and KOMET program.
< < 실시예Example 3 : 통계처리 > 3: Statistical processing>
그래프와 통계는 Excel 2007 (Microsoft, USA)과 SPSS(IBM, USA)를 사용하였다. 각각의 데이터 그룹은 100개의 cell에서 측정된 olive tail moment값의 평균이며 Duncan test를 이용하여 각 그룹의 평균값의 유의성을 검증 (유의수준=0.05로 그림 1: control과 비교, 그림 2~6: 나노 TiO₂ 80ug/ml과 비교한 유의성) 하였다.
Excel 2007 (Microsoft, USA) and SPSS (IBM, USA) were used for the graphs and statistics. Each data group is an average of the olive tail moments measured in 100 cells. The Duncan test is used to verify the significance of the mean values of each group (significance level = 0.05, Figure 1: comparison with control,
< 실험 결과 > < Experimental Results>
도 1은 나노 TiO₂입자 농도별로 임파구 DNA에 대한 독성을 평가한 것이다. comet assay 결과에서 알 수 있듯이 대조군의 olive tail moment 값이 8.79이지만, 20ug/ml 나노 TiO₂입자 처리에 의해서 12.58, 40ug/ml에서는 9.95, 60ug/ml에서는 23.09, 80ug/ml에서는 23.67로 나노 TiO₂입자 증가에 따라 DNA손상이 증가하는 경향을 보였다. Fig. 1 shows the toxicity of lymphocyte DNA to nano TiO2 particle concentration. As shown in the comet assay results, the olive tail moment of the control was 8.79, but it was 12.58 by 20 ug / ml nano TiO 2 particles, 9.95 at 40 ug / ml, 23.09 at 60 ug / ml and 23.67 at 80 ug / ml, DNA damage was observed to be increased according to the number of cells.
도 2 및 도 3은 임파구 DNA 손상이 확인된 나노 TiO₂80ug/ml에서 화학적 활성산소 소거제인 N-acetylcysteine(NAC)와 Vit C의 손상억제효과를 평가한 것이다. 대조군의 olive tail moment 값이 2.80이었지만, 나노 TiO₂80ug/ml을 처리 시 28.09로 확실한 DNA 손상을 유발할 수 있었다. NAC 1ug/ml처리 시 21.22, NAC 3ug/ml에선 20.64, NAC 5ug/ml에선 13.73, NAC 7ug/ml에선 10.97, NAC 9ug/ml에선 6.99 으로 NAC 농도증가에 따라 DNA손상이 억제되었다. FIG. 2 and FIG. 3 are graphs showing inhibitory effects of N-acetylcysteine (NAC) and Vit C, which are chemically reactive oxygen scavengers, at nano TiO 2 80 μg / ml in which lymphocyte DNA damage is confirmed. The olive tail torque value of the control group was 2.80, but the treatment of nano TiO2 at 80ug / ml resulted in a definite DNA damage of 28.09. DNA damage was inhibited by increasing NAC concentration by 21.22 at 1 ug / ml of NAC, 20.64 at 3 ug / ml of NAC, 13.73 at 5 ug / ml of NAC, 10.97 at 7 ug / ml of NAC and 6.99 at 9 ug / ml of NAC.
Vit C 3ug/ml처리 시 14.06, Vit C 5ug/ml 13.31, Vit C 7ug/ml 7.82로 항산화제의 농도가 증가할수록 나노 TiO₂에 의한 세포손상에 대한 억제효과를 얻어낼 수 있었다. 나노 TiO₂80ug/ml에 의한 DNA 손상을 NAC와 Vit C가 억제함으로써 NAC와 Vit C가 나노 TiO₂ 입자에 의한 세포독성을 줄일 수 있음을 알게 되었다.As the concentration of antioxidant increased, the inhibition effect on the cell damage by nano TiO₂ could be obtained with 14.06 Vit C 3 ug / ml, 13.31 Vit C and 13.71 Vit C 7. It was found that NAC and Vit C could reduce the cytotoxicity of nano TiO 2 particles by NAC and Vit C by inhibiting DNA damage by 80 ug / ml of nano TiO 2.
앞의 실험결과를 토대로 천연식물 유래 파이토케미컬인 Resveratol, Sulforaphane, Curcumin에 의한 효과를 살펴보았다. Based on the results of the previous experiments, we examined the effects of natural plant-derived phytochemicals such as Resveratol, Sulforaphane, and Curcumin.
Resveratol은 포도, 라즈베리, 크랜베리와 같은 베리류 등에 포함된 성분으로 폴리페놀의 일종이다. 열악한 환경이나 해충을 만났을 때 유도되기도 하며 항암 및 강력한 항산화작용을 하는 것으로 알려져 있다. 그리고 Sulforaphane은 브로콜리, 양배추 등에 포함된 성분이며 강력한 항산화 효과를 갖고 있다. 또한 위암을 유발하는 인자 중의 하나인 헬리코박터 파이로리의 활성을 억제할 뿐만 아니라 염증 유발인자의 활성을 저해하는 것으로 알려져 있다. Curcumin은 카레의 노란빛을 띠는 색소로 강황에 포함되어 있는 성분이다. 항암, 항산화, 항염증의 효과와 함께 장 기능을 강화시키고 치매, 비만 예방에도 효과적이다.Resveratol is a component of polyphenols, a component of grape, raspberry, cranberry and other berries. It is induced when it meets poor environment or pest, and it is known to have anticancer and powerful antioxidant effect. And Sulforaphane is a component contained in broccoli, cabbage and has a strong antioxidant effect. It is also known to inhibit the activity of Helicobacter pylori, one of the factors causing gastric cancer, as well as to inhibit the activity of inflammatory factors. Curcumin is a yellowish coloring of curry and is a component of turmeric. Anticancer, antioxidant, anti-inflammatory effect, as well as strengthen the bowel function, dementia, is also effective in preventing obesity.
이 파이토케미컬이 나노 TiO₂에 대한 독성을 감소시킬 수 있는지 확인해보았다. We have confirmed that this phytochemical can reduce the toxicity to nano TiO₂.
도 4 내지 6은 임파구 DNA 손상이 확인된 나노 TiO₂80ug/ml을 조건으로 하여 Resveratrol, Sulforaphane, Curcumin을 다양한 농도로 처리하여 TiO2 독성을 억제할 수 있는지 효능을 평가한 것이다. 이 또한 comet assay를 통해 세포 독성을 평가하였다. FIGS. 4 to 6 show that Resveratrol, Sulforaphane, and Curcumin were treated at various concentrations under the condition of
도 4에서는 대조군의 olive tail moment 값이 2.36이었고 나노 TiO₂ 80ug/ml에 의한 DNA 손상으로 olive tail moment가 21.98로 측정되었다. 그 결과 Resveratol 3, 5, 7 ug/ml에 의해서 olive tail moment 값이 현저하게 감소되었다. In Fig. 4, the olive tail moment of the control group was 2.36, and the olive tail moment was measured as 21.98 due to DNA damage caused by 80 ug / ml of nano TiO2. As a result, olive tail moment values were significantly decreased by
마찬가지로 Sulforaphane 1, 2, 3 ug/ml에 의해서 임파구 DNA 손상이 크게 억제되었고(도 5) Curcumin 0.5, 1, 2, 3 ug/ml에 의해서는 임파구 DNA 손상이 크게 억제되었다. Similarly, lymphocyte DNA damage was greatly suppressed by Sulforaphane 1, 2, and 3 ug / ml (Fig. 5), and lymphocyte DNA damage was significantly inhibited by Curcumin 0.5, 1, 2, and 3 ug / ml.
이러한 결과는 TiO₂ 나노입자에 의한 임파구 DNA 손상을 억제하는데 비타민 C 와 NAC 항산화제뿐만 아니라 resveratrol, Sulforaphane, Curcumin 등의 파이토케미컬이 효과적임을 나타내는 것이다. These results demonstrate that phytochemicals such as resveratrol, sulforaphane, and curcumin, as well as vitamin C and NAC antioxidants, are effective in suppressing lymphocyte DNA damage by TiO2 nanoparticles.
상기에 제시된 실시예는 예시적인 것으로 이 분야에서 통상의 지식을 가지는 자는 본 발명의 기술적 사상을 벗어나지 않는 범위에서 제시된 실시예에 대한 다양한 변형 및 수정 고안을 만들 수 있을 것이다. 이러한 변형 및 수정 고안에 의하여 본 발명의 범위는 제한되지 않는다.
The embodiments presented above are illustrative and those skilled in the art will be able to make various modifications and alterations to the disclosed embodiments without departing from the technical spirit of the present invention. The scope of the present invention is not limited by these variations and modifications.
Claims (5)
상기 천연 파이토케미컬은 레스베라트롤(resveratrol) 또는 설포라판(Sulforaphane) 또는 커큐민(Curcumin) 중 어느 하나 이상을 포함하는 Nanosize TiO₂독성에 의한 DNA 손상 예방 및 치료용 조성물. The method according to claim 1,
Wherein the natural phytochemical comprises at least one of resveratrol, sulforaphane, or curcumin, and a composition for preventing and treating DNA damage by Nanosize TiO 2 toxicity.
항산화제를 더 포함하는 Nanosize TiO₂독성에 의한 DNA 손상 예방 및 치료용 조성물. The method according to claim 1,
A composition for preventing and treating DNA damage by Nanosize TiO2 toxicity further comprising an antioxidant.
상기 항산화제는 비타민 C 또는 N-acetylcysteine(NAC) 인 것을 특징으로 하는 Nanosize TiO₂독성에 의한 DNA 손상 예방 및 치료용 조성물. The method according to claim 1,
Wherein the antioxidant is vitamin C or N-acetylcysteine (NAC). The composition for preventing and treating DNA damage by nanosize TiO 2 toxicity.
A composition for preventing and treating DNA damage by Nanosize TiO2 toxicity comprising an antioxidant.
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