KR20150008777A - Slide Assembly - Google Patents

Slide Assembly Download PDF

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KR20150008777A
KR20150008777A KR1020130083176A KR20130083176A KR20150008777A KR 20150008777 A KR20150008777 A KR 20150008777A KR 1020130083176 A KR1020130083176 A KR 1020130083176A KR 20130083176 A KR20130083176 A KR 20130083176A KR 20150008777 A KR20150008777 A KR 20150008777A
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slide
slide assembly
cells
mounting hole
present
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KR1020130083176A
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KR101770991B1 (en
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전병희
이종길
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주식회사 싸이토젠
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Priority to KR1020130083176A priority Critical patent/KR101770991B1/en
Priority to PCT/KR2014/006306 priority patent/WO2015008991A1/en
Priority to US14/905,273 priority patent/US9739691B2/en
Priority to JP2016527918A priority patent/JP6247391B2/en
Publication of KR20150008777A publication Critical patent/KR20150008777A/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36—Embedding or analogous mounting of samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00138—Slides

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
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  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
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  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention provides a slide assembly for cytospin. The slide assembly according to the present invention comprises a slide which has a mounting unit having an open top and discharge holes on the wall and protruding from the upper surface; a filter card arranged at the upper part of the slide and having a hole to insert the mounting unit therein; and a collecting member with an open top having a mesh filter at the lower part and fitting in the mounting unit. According to the present invention, the slide assembly has the effects of preventing the loss of cells to be examined, and precisely allowing quantitative and qualitative analysis of a subject to be examined.

Description

슬라이드 조립체{Slide Assembly}BACKGROUND OF THE INVENTION 1. Field of the Invention [0001]

본 발명은 슬라이드 조립체에 관한 것으로, 보다 상세히는 사이토스핀(Cytospin)용 슬라이드 조립체에 관한 것이다.The present invention relates to a slide assembly, and more particularly to a slide assembly for Cytospin.

잘 알려진 바와 같이, 사이토스핀은 원심력을 이용하여 액체성 검체에 포함된 세포를 한정된 부위의 슬라이드에 잘 펴서 농축하는 기기이다. 사이토스핀에 의하여 농축된 세포들은 직접 슬라이드 위의 소정의 위치에 모여져 도말(塗抹)된다. 이와 같이, 사이토스핀은 한 곳으로 검사하고자 하는 세포를 모아주는 역할을 하기 때문에 염색후에 관찰하면, 검사대상의 세포의 모양 성상을 확연하게 볼 수 있게 된다. 특히, 사이토스핀은 적은 양의 액체성 검체에 세포의 수가 적을 때 가장 효과적이며, 세포 생물학, 조직학, 병리학등의 분야에 널리 사용되는 의료장비이다. As is well known, cyto-spin is a device for concentrating the cells contained in a liquid specimen using a centrifugal force by spreading the cells on a limited area of the slide well. The cells concentrated by the cytospin are collected directly at predetermined positions on the slide. As described above, since the cytospin functions to collect the cells to be examined in one place, the appearance of the cells to be inspected can be clearly seen by observation after dyeing. In particular, cytospin is most effective when the number of cells is small in a small amount of liquid specimen, and is widely used in fields such as cell biology, histology, and pathology.

이와 같은 사이토스핀에 사용되는 종래의 슬라이드 조립체는 도 1a와 도 1b에 도시되어 있다. 도시되어 있는 바와 같이, 종래의 슬라이드 조립체(1)는 하부에 슬라이드(2)가 위치하고, 슬라이드(2)의 상면에는 액체를 흡수하는 흡수지인 필터카드(Filter Card, 3)가 배치되어 있다. 필터카드(3)는 구멍(3a)이 천공되며, 하부의 슬라이드(2)와 협력하여 수용공간(3b)을 형성한다. 사이토스핀의 작동에 의하여 슬라이드(2)로 분출되는 액체가 수용공간(3b)에 진입하여 액체내의 세포가 슬라이드(2)에 부착되고, 액체는 도 1b에 화살표로 나타낸 바와 같이 필터카드(3)에 흡수된다. A conventional slide assembly for such a cyto-spin is shown in Figs. 1A and 1B. As shown in the drawing, a conventional slide assembly 1 has a slide 2 disposed thereunder, and a filter card 3, which is an absorbent for absorbing liquid, is disposed on the slide 2. The filter card 3 is drilled in the hole 3a and cooperates with the lower slide 2 to form a receiving space 3b. The liquid ejected to the slide 2 enters the accommodation space 3b and the cells in the liquid adhere to the slide 2 by the operation of the cyto-spin, and the liquid is introduced into the filter card 3 as indicated by arrows in Fig. .

그러나, 상기한 바와 같은 종래의 슬라이드 조립체에 있어서, 수용공간(3b)내의 일부 세포들도 액체와 함께 필터카드에 흡수되어, 세포의 손실이 발생한다. 특히, 시료내의 세포의 개수가 중요한 경우에는 이와 같은 세포의 손실은 검사 결과의 판독에 있어서 심각한 오류를 초래하는 문제점이 있다. 즉, 검사 대상의 세포의 손실을 초래함으로써 탐지되어야 할 세포들이 제외되어 분석 목표의 상당한 부분을 놓치게 되어 양적 및 질적 결과에 악영향을 미칠 수 있게 된다.However, in the conventional slide assembly as described above, some of the cells in the accommodation space 3b are also absorbed by the filter card together with the liquid, resulting in loss of cells. Particularly, when the number of cells in a sample is important, the loss of such cells causes a serious error in reading the test results. That is, by causing the cells to be inspected to be lost, cells that should be detected are excluded and a large part of the analysis target is missed, which can adversely affect quantitative and qualitative results.

이와 같이 본 발명은 상기한 종래 기술의 문제점을 해결하기 위하여 안출된 것으로, 본 발명의 목적은 검사 대상의 세포들의 손실을 방지하는 슬라이드 조립체를 제공함에 있다.SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems of the prior art, and it is an object of the present invention to provide a slide assembly for preventing loss of cells to be inspected.

본 발명의 다른 목적은 검사대상인 세포들의 손실 방지에 따라 검사 대상에 대한 정량적이고 정성적인 분석을 정확하게 제공할 수 있는 슬라이드 조립체를 제공함에 있다.Another object of the present invention is to provide a slide assembly which can accurately provide quantitative and qualitative analysis of an object to be inspected according to prevention of loss of cells to be inspected.

상기한 목적들을 달성하기 위한 본 발명에 따른 슬라이드 조립체는 상부가 개방되고 벽면에 배출구멍이 형성되어 있는 장착구가 상면에 돌출설치되어 있는 슬라이드와; 이 슬라이드의 상부에 배치되고 장착구가 끼워지는 구멍이 형성되어 있는 필터카드와; 상부는 개방되고 하부에는 메시필터가 설치되어 있으며, 장착구의 내부에 끼워맞춤되는 포집부재로 이루어져 있음에 특징이 있다.According to an aspect of the present invention, there is provided a slide assembly comprising: a slide having an upper opening and a mounting hole protruding from an upper surface of the slide assembly; A filter card disposed at an upper portion of the slide and having a hole through which the mounting hole is fitted; Characterized in that the upper part is opened and the mesh filter is provided in the lower part, and the collecting member is fitted into the inside of the mounting hole.

본 발명에 따른 슬라이드 조립체는 검사 대상인 세포들의 손실을 방지할 수 있는 효과가 있다.The slide assembly according to the present invention has an effect of preventing the loss of cells to be inspected.

본 발명에 따른 슬라이드 조립체는 검사 대상에 대한 정량적이고 정성적인 분석을 정확하게 제공할 수 있는 효과가 있다.The slide assembly according to the present invention has the effect of accurately providing quantitative and qualitative analysis of an object to be inspected.

도 1a는 종래에 따른 슬라이드 조립체의 분해사시도,
도 1b는 종래에 따른 슬라이드 조립체의 단면도,
도 2는 본 발명에 따른 슬라이드 조립체의 분해사시도,
도 3은 본 발명에 따른 슬라이드 조립체의 선 A-A'에 따른 부분절개 확대단면사시도이다.FIG. 1A is an exploded perspective view of a conventional slide assembly,
1B is a cross-sectional view of a conventional slide assembly,
2 is an exploded perspective view of a slide assembly according to the present invention,
3 is a partially cut-away enlarged cross-sectional perspective view of the slide assembly according to the present invention taken along line A-A '.

본 발명의 그 밖의 목적, 특정한 장점들과 신규한 특징들은 첨부된 도면들과 연관되어지는 이하의 상세한 설명과 바람직한 실시예로부터 더욱 분명해질 것이다.Other objects, specific advantages and novel features of the present invention will become more apparent from the following detailed description of preferred embodiments with reference to the accompanying drawings.

이하에서는, 본 발명에 따른 슬라이드 조립체의 실시예를 첨부한 도면을 참고로 하여 상세히 설명한다. Hereinafter, embodiments of a slide assembly according to the present invention will be described in detail with reference to the accompanying drawings.

도 2를 참고하면, 본 발명에 따른 슬라이드 조립체는 하부에는 슬라이드(10)가 위치하고, 슬라이드(10)의 상부에는 흡수지인 필터카드(20)가 슬라이드(10)의 상면에 맞닿아 배치되어 있다. 그리고, 필터카드(20)의 상부에는 포집부재(30)가 위치하고 있다.Referring to FIG. 2, a slide assembly 10 according to the present invention has a slide 10 disposed thereunder, and a filter card 20, which is an absorbent, is disposed on the slide 10 in contact with the upper surface of the slide 10. The collecting member 30 is located above the filter card 20.

슬라이드(10)는 도 2 및 도 3에 도시되어 있는 바와 같이, 상면에 장착구(12)가 돌출설치되어 있다. 장착구(12)는 상부는 개방되어 있고, 위쪽으로 갈수록 지름이 커지는 단면이 테이퍼진 형상을 갖는다. 장착구(12)의 하부 벽면에는 배출구멍(14)들이 일정한 간격을 두고 형성되어 있다. 본 실시예에 서는 다수의 배출구멍(14)들이 형성되어 있으나, 배출구멍은 길게 연장된 단일의 구멍으로도 형성할 수 있다. As shown in Figs. 2 and 3, the slide 10 is provided with a mounting hole 12 protruding from its upper surface. The mounting hole 12 is open at the top and has a tapered cross section whose diameter increases toward the upper side. On the lower wall surface of the mounting hole 12, discharge holes 14 are formed at regular intervals. In this embodiment, a plurality of discharge holes 14 are formed, but the discharge hole can also be formed as a single long hole.

필터카드(20)는 슬라이드(10)의 상면에 놓여지며, 장착구(12)가 끼워지도록 장착구와 정렬되는 끼움구멍(22)이 형성되어 있다. 장착구(12)가 끼움구멍(22)에 삽입되면 장착구(12)는 필터카드(20)의 상부로 돌출되어 노출된다. The filter card 20 is placed on the upper surface of the slide 10 and has a fitting hole 22 aligned with the mounting hole so that the fitting hole 12 is fitted. When the mounting hole 12 is inserted into the fitting hole 22, the mounting hole 12 protrudes to the upper portion of the filter card 20 and is exposed.

포집부재(30)는 슬라이드(10)의 장착구(12)에 끼워맞춤되어 고정된다. 포집부재(30)의 상부는 개방되어 있고, 장착구(12)와 동일한 형상으로 위쪽으로 갈수록 지름이 커지는 단면이 테이퍼진 형상을 갖는다. 포집부재(30)는 장착구(12)와 동일하게 장착구(12)에 끼워맞춤되어 필터카드(20)의 상부로 돌출되어 노출되어 있다. 포집부재(30)의 하부에는 메시필터(32)가 고정적으로 설치되어 있다. 메시필터(32)에는 검사 대상의 세포들을 여과시킬 수 있는 크기를 갖는 복수의 여과구멍(32a)들이 형성되어 있다. 이와 같은 구성을 갖는 포집부재(30)는 메시필터(32)에 의하여 세포(C)들이 포집되는 수용공간(34)이 형성된다. The collecting member 30 is fitted and fixed to the mounting hole 12 of the slide 10. The upper portion of the collecting member 30 is open and has the same shape as the mounting hole 12 and has a tapered cross section whose diameter increases toward the upper side. The collecting member 30 is fitted to the mounting hole 12 and exposed to the upper portion of the filter card 20 in the same manner as the mounting hole 12. A mesh filter (32) is fixedly installed below the collecting member (30). The mesh filter 32 is formed with a plurality of filtering holes 32a having a size capable of filtering cells to be inspected. In the collecting member 30 having such a configuration, a receiving space 34 in which the cells C are collected by the mesh filter 32 is formed.

한편, 바람직하게, 메시필터(32)에는 세포의 포집을 용이하게 할 수 있도록 표면이 코팅처리되어 있다.On the other hand, preferably, the mesh filter 32 is coated with a surface so as to facilitate cell collection.

지금부터는, 상기한 바와 같은 구성을 갖는 본 발명에 따른 슬라이드 조립체의 작용에 대하여 설명한다.Hereinafter, the operation of the slide assembly according to the present invention having the above-described configuration will be described.

먼저, 사이토스핀을 작동시켜 원심력에 의하여 검사 대상의 액체를 분출시킨다. 그에 따라, 도 3을 참고하면, 화살표로 나타낸 바와 같이, 분출된 세포들이 포함된 액체가 포집부재(30)의 내부인 수용공간(34)로 진입하고, 계속하여 하부로 이동한다. 액체는 여과구멍(32a)을 통과하여 장착구(12)의 하부로 유동하고, 계속하여 장착구(12)의 벽면에 형성된 배출구멍(14)로 흘러나가 필터카드(20)로 흡수된다. 그리고, 검사 대상인 세포(C)들은 여과구멍(32a)를 통과하지 못하고 여과되어 세포들(C)은 수용공간(34)내에 포집된다. First, the cyto-spins are operated to eject the liquid to be inspected by centrifugal force. Accordingly, referring to FIG. 3, as shown by the arrow, the liquid containing the ejected cells enters the receiving space 34 inside the collecting member 30 and moves to the lower part continuously. The liquid flows through the filtration hole 32a to the lower portion of the mounting hole 12 and then flows to the discharge hole 14 formed in the wall surface of the mounting hole 12 and is absorbed by the filter card 20. The cells C to be inspected are filtered without passing through the filtering holes 32a, and the cells C are collected in the receiving space 34. [

본 발명에 따라, 세포(C)들이 포집부재(30)의 수용공간(34)에 손실없이 포집되고, 포집된 세포(C)들은 예정된 다음의 검사단계로 보내어지게 된다. According to the present invention, the cells C are collected without loss in the receiving space 34 of the collecting member 30, and the collected cells C are sent to a predetermined next inspection step.

이상에서 설명된 실시예는 본 발명의 바람직한 실시예를 설명한 것에 불과하고, 본 발명의 권리범위는 설명된 실시예에 한정되는 것은 아니며, 본 발명의 기술적 사상과 특허청구범위 내에서 이 분야의 당업자에 의하여 다양한 변경, 변형 또는 치환이 가능할 것이며, 그와 같은 실시예들은 본 발명의 범위에 속하는 것으로 이해되어야 한다.While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

10: 슬라이드 12: 장착구
14: 배출구멍 20: 필터카드
30: 포집부재 32: 메시필터
34: 수용공간 C: 세포10: Slide 12: Mounting hole
14: exhaust hole 20: filter card
30: collecting member 32: mesh filter
34: receptive space C: cell

Claims (4)

상부가 개방되고 벽면에 배출구멍이 형성되어 있는 장착구가 상면에 돌출설치되어 있는 슬라이드와;
이 슬라이드의 상부에 배치되고 상기 장착구가 끼워지는 구멍이 형성되어 있는 필터카드와;
상부는 개방되고 하부에는 메시필터가 설치되어 있으며, 상기 장착구의 내부에 끼워맞춤되는 포집부재로 이루어진 슬라이드 조립체.A slide having an upper portion thereof opened and a mounting hole having a discharge hole formed in the wall thereof so as to protrude from the upper surface thereof;
A filter card disposed at an upper portion of the slide and having a hole through which the mounting hole is inserted;
And a collecting member fitted to the inside of the mounting hole, the mesh filter being installed in the lower part of the slide assembly. 제1항에 있어서, 상기 포집부재의 상면이 상기 필터카드의 상면보다 높은 위치에 있는 슬라이드 조립체.The slide assembly according to claim 1, wherein the upper surface of the collecting member is higher than the upper surface of the filter card. 제2항에 있어서, 상기 장착구와 상기 포집부재는 단면이 테이퍼진 슬라이드 조립체.3. The slide assembly of claim 2, wherein the mounting aperture and the collecting member are tapered in cross-section. 제1항에 또는 제3항에 있어서, 상기 장착구의 배출구멍은 원주상으로 형성되어 있는 슬라이드 조립체.The slide assembly according to claim 1 or 3, wherein the discharge hole of the mounting hole is circumferentially formed.
KR1020130083176A 2013-07-15 2013-07-15 Slide Assembly KR101770991B1 (en)

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US14/905,273 US9739691B2 (en) 2013-07-15 2014-07-15 Slide assembly
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