KR20150000208A - Fruit antioxidant compositions containing of that include Litsea japonica Fruit from essential oil is extracted - Google Patents
Fruit antioxidant compositions containing of that include Litsea japonica Fruit from essential oil is extracted Download PDFInfo
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- KR20150000208A KR20150000208A KR1020130072368A KR20130072368A KR20150000208A KR 20150000208 A KR20150000208 A KR 20150000208A KR 1020130072368 A KR1020130072368 A KR 1020130072368A KR 20130072368 A KR20130072368 A KR 20130072368A KR 20150000208 A KR20150000208 A KR 20150000208A
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- South Korea
- Prior art keywords
- essential oil
- fruit
- oil component
- pure
- seed
- Prior art date
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Abstract
Description
본 발명은 까마귀쪽나무 오일을 이용한 항산화조성물에 관한 것으로, 상세하게는 까마귀쪽나무의 열매로부터 획득되는 정유성분을 함유하는 항산화조성물에 관한 것이다.
The present invention relates to antioxidative compositions using wood oil of crows, and more particularly to an antioxidant composition containing an essential oil component obtained from the fruit of a crow tree.
체내에서 생성되는 활성산소종(reactive oxygen species, ROS)은 에너지대사, 면역반응, 신경의 전기적인 신호전달 등의 순기능을 가지고 있지만 과도하게 생성된 활성산소종은 단백질 및 DNA의 변형, 생체막과 조직을 손상시켜 노화, 대사성질환, 암 유발 등 부정적인 기능을 하는 것으로 알려져 있다. 과도하게 생성된 활성산소종을 저감하기 위하여 체내에는 SOD(superoxide dismutase), glutathione peroxidase(GPx) 및 catalase 등과 같은 항산화 시스템(antioxidant systems)이 존재하지만 현대인의 경우에는 스트레스, 화학물질 및 환경호르몬에 노출빈도가 높기 때문에 체내 항산화 효소 시스템을 유지시켜주기 위한 기능성 소재개발이 지속적으로 대두되고 있다. The reactive oxygen species (ROS) generated in the body have a net function such as energy metabolism, immune response, electrical signal transmission of the nerve, but the excessively produced reactive oxygen species are protein and DNA deformation, And it is known to have negative functions such as aging, metabolic diseases, and cancer induction. Antioxidant systems such as superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase are present in the body to reduce excessively active oxygen species, but modern humans are exposed to stress, chemicals and environmental hormones Because of the high frequency, the development of functional materials to maintain the antioxidant enzyme system in the body is continuously being developed.
최근 천연물로부터 체내 항산화시스템을 유지시켜 주는 천연 항산화제에 대한 개발이 활발하게 이루어지고 있다. Recently, natural antioxidants that maintain the body's antioxidant system from natural products have been actively developed.
식물에는 비타민C, 토코페롤, 카로티노이드, 페놀성 화합물과 같은 이차 대사산물이 존재하는데, 이들은 높은 항산화활성을 나타내며 오랜 기간 인류가 안전하게 이용해왔다. Plants have secondary metabolites such as vitamin C, tocopherol, carotenoids, and phenolic compounds, which have high antioxidant activity and have been used safely for a long time.
특히, 제주도의 경우 다른 지역에 비해 다양한 종류의 생물자원들이 자생하고 있고, 지리학적으로 남방식물의 북방 한계선임과 동시에 북방식물의 남방한계선으로써 두 지역의 식물이 공존하는 장소이다. In particular, Jeju Island is a place where diverse kinds of biological resources are naturally grown compared to other regions, geographically, the northern limit of southern plants is selected and the southern limit of northern plants is coexistence of the two regions.
이와 같은 이유로 제주도의 생물은 생육 온도보다 고온 또는 저온의 스트레스에 노출되기 때문에 다른 지역의 생물보다 이차 대사산물의 함량이 높은 것으로 알려져 있다.For this reason, it is known that the biomass of Jeju Island is exposed to higher or lower stress than the growth temperature, so that the content of secondary metabolites is higher than that of other regions.
본 발명에 사용되는 까마귀쪽나무(Litsea japonica)는 상록엽소교목으로서 수직적으로 표고 700m 이내의 바닷가 및 산기슭에 주로 분포한다. Litsea japonica used in the present invention is an evergreen tree species and is distributed vertically in the beaches and at the foot of mountains within 700 m above sea level.
이 식물의 화학적 구성에 대한 기존의 연구에서, 여러 종류의 essential oils, fatty acids, lactones, alkaloids 및 terpenoids가 발견되었다고 보고되어있다. Previous studies on the chemical composition of this plant have reported several types of essential oils, fatty acids, lactones, alkaloids and terpenoids.
Lactones의 종류로 알려진 hamabiwalactone A, hamabiwalatone B, akolactone B, litsealactone A 및 litsealactoneB가 분리되었다. Hamabiwalactone A, hamabiwalatone B, akolactone B, litsealactone A and litsealactone B, which are known as Lactones, were isolated.
또한, 잎으로부터 분리된 flavonoids 성분인 epicatechin, afzelin, quercitrin 및 tiliroside의 anti-complement activity를 조사한 결과 및 tiliroside가 complement system에 대해 가장 강력한 억제작용을 나타냄이 보고되어있다. In addition, the anti-complement activity of epicatechin, afzelin, quercitrin and tiliroside, which are flavonoids isolated from leaves, has been investigated, and tiliroside has been reported to exhibit the strongest inhibitory action on the complement system.
까마귀쪽나무 잎 80% EtOH 추출물을 분획 후, 분획물을 사용하여 항산화(DPPH, Xanthine Oxidase, Superoxide radical -> 분획물 EtOAC 층에서 DPPH와 Superoxid radical 억제활성이 우수함) 및 LDH, NO, PGE2(함염)을 측정(윤원종 외, 2010, J, Korean Soc. Appl. Biol. Chem. 53(1): p 27~32)하였으며, 실리카 컬럼을 이용하여 잎의 플라보노이드종 분리 (Epicatechin, Afzlin, Quercitrin, Tiliroside, Myricitrin, Kaempferol, Quercetin, Myricetin) 후 Anti-complement system 활성 측정(Sun Young Lee 외, 2005, PHYTOTHERAPY RESEARCH, 19 : p. 273-276)하였고, 식용 및 약용식물 중 까마귀쪽나무 잎을 메탄올을 이용하여 추출 후 과산화지질 생성억제 측정(박종철 외, 1997, J. Korean Soc. Food Sci. Nutr. 26(6) : p. 1159-1163)하는 연구에서 까마귀쪽나무의 경우 활성도는 10-1 mg/mL 농도에서 약 18%의 억제효과를 보인 것으로 보고하였다. (DPPH, Xanthine Oxidase, Superoxide radical -> Supernox radical -> Supernox radical scavenging activity in the fraction EtOAC layer) and LDH, NO, PGE 2 (impurities) in fractions of 80% (Epicatechin, Afzlin, Quercitrin, Tiliroside, and Pyridyl) by using silica column, and measuring the amount of flavonoids in leaves (Epicatechin, Afzlin, Quercitrin, Tiliroside, The results of this study were as follows: 1) Anti-complement system activity was measured after Myricitrin, Kaempferol, Quercetin, Myricetin, and PHYTOTHERAPY RESEARCH, In the study, the activities of the crows were 10 -1 mg / mL (in the case of the crow tree) And the inhibitory effect was about 18%.
또한, 까마귀쪽나무(Litsea japonica)의 HL-60 백혈병 세포 Apoptosis 유도효과에 대한 연구(2009, 김엘비라 외, Ykhak Hoeji, 53(1) : p. 6-11)에서 까마귀쪽나무 잎을 이용하여 80% 에탄올을 이용하여 추출하고, HL-60(세포독성)-MTT 측정결과 정상세포에는 독성을 갖지 않으며, HL-60(백혈병세포) 세포증식억제효과를 보임, DNA fragmentation 분석, Western blot 분석을 통하여 Bcl-2의 발현 감소 및 caspase를 활성화함으로서 apoptosis를 유도하여 증식을 저해효과를 보고하는 등 까마귀쪽나무에 대한 연구가 일부 이루어졌다.In addition, in a study on the induction effect of HL-60 leukemia cell apoptosis in a crow tree (Litsea japonica) (2009, Kim, Elvira et al., Ykhak Hoeji, 53 (1) HL-60 (cytotoxicity) -MTT measurement showed that the cells were not toxic to normal cells and showed HL-60 (leukemia cell) cell proliferation inhibition effect, DNA fragmentation analysis, Western blot analysis The expression of Bcl-2 and the activation of caspase induced apoptosis and inhibited the proliferation of Bcl-2.
특허문헌의 종래기술로는 (KR) 공개특허 10-2008-0020738(공개일 2008.03.06)호의 항염활성 및 골대사 관련인자 억제활성을 나타내는 까마귀쪽나무 추출물에 관한 기술과, (KR) 공개특허 10-2009-0091477(공개일 2009.08.28)호의 까마귀쪽나무 추출물을 유효성분으로 함유하는 암 질환의 예방 및 치료용 조성물에 관한 기술과, (KR) 등록특허 10-1243243(등록일 2012.03.07)호의 까마귀쪽나무 추출물 또는 이의 분획물을 유효성분으로 함유하는 당뇨합병증 예방 및 치료용 조성물에 관한 기술이 제공되어 있다.
The prior art of the patent literature is a technique relating to the extract of a crow tree of a crow, which exhibits anti-inflammatory activity and inhibitory effect on bone metabolism-related factors of (KR) Patent Publication No. 10-2008-0020738 (published on Mar. -2009-0091477 (Publication Date 2009.08.28), which is incorporated by reference herein in its entirety, and a composition for preventing and treating cancer diseases containing as an active ingredient a root extract of a crow There is provided a technique for a composition for preventing and treating diabetic complications, which comprises an extract of Raven or a fraction thereof as an active ingredient.
본 발명은 항산화효과를 가지는 까마귀쪽나무 열매의 정유성분을 유효성분으로 함유하는 항산화효과를 가지는 조성물을 제공하고자 한다.The present invention provides a composition having an antioxidative effect containing an essential oil component of a nuts tree fruit having an antioxidant effect as an active ingredient.
즉, 본 출원인은 항산화효과를 갖는 소재를 개발하기 위하여 천연물로부터 스크리닝하던 중, 까마귀쪽나무 열매의 정유성분(오일)이 DPPH radical scavenging activity 평가 및 총 페놀함량측정 등을 통하여 항산화효과를 확인하여 까마귀쪽나무 열매의 정유성분을 유효성분으로 함유하는 항산화효과를 갖는 조성물을 제공하고자 한다.
In other words, in order to develop a material having antioxidative effect, the present applicant confirmed the antioxidative effect through the evaluation of the DPPH radical scavenging activity and the measurement of the total phenol content, while the essential oil (oil) of the nuts tree fruit was screened from natural products, To provide a composition having an antioxidative effect containing an essential oil component of an effective nutrient as an active ingredient.
상기와 같은 목적을 달성하고자 본 발명인 항산화효과를 가지는 항산화조성물은 까마귀쪽나무의 열매로부터 추출되는 정유성분을 함유함을 특징으로 한다.In order to achieve the above object, the antioxidant composition having antioxidative effect according to the present invention is characterized by containing an essential oil component extracted from the fruit of a crow tree.
상기 정유성분은 까마귀쪽나무의 순수열매(종자를 제거한 것) 또는 종자열매(종자를 포함한 것) 또는 종자(열매의 씨앗) 중 어느 하나로부터 획득한 것임을 특징으로 한다.Wherein the essential oil component is obtained from pure fruit (removed seed) or seed fruit (including seed) or seed (fruit seed) of a crow tree.
또는, 상기 정유성분은 까마귀쪽나무의 순수열매로 획득되는 순수열매 정유성분과, 까마귀쪽나무의 종자로 획득되는 종자 정유성분이며, 상기 순수열매 정유성분과 종자 정유성분은 단독 또는 혼합사용될 수 있다.Alternatively, the essential oil component is a pure essential oil component obtained by pure fruit of a crow tree and a seed essential oil component obtained by a seed of a crow tree, and the pure fruit essential oil component and the seed essential oil component may be used singly or in combination .
상기 정유성분은 주정추출법, 헥산(hexane)추출법, 초임계추출법, 증류법, 압착법 중 어느 하나의 방법으로 획득된다.The essential oil component is obtained by any one of the following methods: alcohol extraction, hexane extraction, supercritical extraction, distillation, and compression.
항산화 조성물용 까마귀쪽나무의 열매 정유성분의 제조방법은 까마귀쪽나무의 순수열매 또는 종자를 용매인 주정(EtOH) 95%을 이용하여 추출하고 알코올을 제거한 다음 물을 넣어 층 분리 등의 공정을 포함한 방법과 합성흡착제 등을 이용하여 획득할 수 있으며, 또는 까마귀쪽나무의 순수열매 또는 종자를 용매인 노말헥산(n-hexane)을 중량대비 9~11배수를 투입하고, 상온에서 24시간 동안 교반시켜 추출하고, 헥산(hexane) 층을 제거하여 획득될 수 있다.
For the preparation of the essential oil component of the crow's side tree for antioxidant composition, pure fruit or seed of the crow tree is extracted using 95% EtOH solvent, alcohol is removed, water is added, Or n-hexane (n-hexane), which is a solvent for the pure fruit or seed of a crow tree, is added to the mixture in an amount of 9 to 11 times by weight, and the mixture is stirred at room temperature for 24 hours Extraction, and removal of the hexane layer.
본 발명의 까마귀쪽나무 열매로부터 획득되는 열매의 정유성분은 DPPH radical scavenging activity 평가 및 총 페놀함량측정 등으로 항산화효과를 확인하여 항산화효과 조성물의 유효성분으로 유용하게 사용될 수 있다. The essential oil component of the fruit obtained from the crow's nuts of the present invention can be effectively used as an active ingredient of the antioxidant effect composition by confirming the antioxidative effect by the evaluation of the DPPH radical scavenging activity and the measurement of the total phenol content.
도 1은 본 발명의 실시 예에 따른 까마귀쪽나무 열매로부터 획득되는 순수열매 정유성분과 종자 정유성분의 총 페놀함량을 측정하여 나타낸 그래프.
도 2는 본 발명의 실시 예에 따른 까마귀쪽나무 열매로부터 획득되는 순수열매 정유성분과 종자 정유성분의 DPPH radical scavenging activity 평가를 나타낸 그래프.
도 3은 본 발명의 실시 예1에 따른 까마귀쪽나무 열매로부터 획득되는 순수열매 정유성분의 크로마토그래프.
도 4는 본 발명의 실시 예2에 따른 까마귀쪽나무 열매로부터 획득되는 순수열매 정유성분의 크로마토그래프.BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the total phenolic content of pure essential oil and seed essential oil components obtained from corn root fruit according to an embodiment of the present invention. FIG.
FIG. 2 is a graph showing DPPH radical scavenging activity evaluation of pure fruit essential oil component and seed essential oil component obtained from corn root nuts according to an embodiment of the present invention.
FIG. 3 is a chromatogram of a pure fruit essential oil component obtained from corn root fruit according to Example 1 of the present invention. FIG.
FIG. 4 is a chromatogram of a pure fruit essential oil component obtained from corn root fruit according to Example 2 of the present invention. FIG.
본 명세서 및 청구범위에 사용된 용어나 단어는 통상적이거나 사전적인 의미로 한정해서 해석되어서는 아니 될 것이며, 본 명세서에 기재된 실시 예와 도면에 도시된 구성은 본 발명의 가장 바람직한 일 실시 예에 불과할 뿐이고 본 발명의 기술적 사상을 모두 대변하는 것은 아니므로, 본 출원시점에 있어서 이들을 대체할 수 있는 다양한 균등물과 변형 예들이 있을 수 있음을 이해하여야 한다.
It is to be understood that the terms and words used in the present specification and claims should not be construed as limited to a conventional or dictionary sense and that the configurations shown in the embodiments and drawings described herein are only the most preferred embodiments of the present invention It is to be understood that the invention is not limited to the specific embodiments disclosed herein, and that various equivalents and modifications may be made thereto without departing from the scope of the present invention.
본 발명은 까마귀쪽나무의 열매로부터 획득되는 정유성분을 유효성분으로 함유하는 항산화 조성물 및 제조방법을 제공한다.
The present invention provides an antioxidative composition containing an essential oil component obtained from the fruit of a crow tree, as an active ingredient, and a preparation method thereof.
이하, 본 발명에 대하여 상세히 설명한다.Hereinafter, the present invention will be described in detail.
본 발명에 따른 조성물의 유효성분인 까마귀쪽나무의 열매로부터 획득되는 정유성분은 종자(열매 속의 씨앗)를 분리한 열매(이하, '순수열매'라 칭함), 종자(열매 속의 씨앗)로 분리하고, 이로부터 정유성분을 획득하였으며, 하기와 같은 방법으로 제조된다. The essential oil component obtained from the fruits of the crow tree, which is an active ingredient of the composition according to the present invention, is obtained by separating the seeds (seeds in the fruit) into separated fruits (hereinafter referred to as 'pure fruit') and seeds (seeds in the fruit) , And an essential oil component was obtained therefrom.
그러나 까마귀쪽나무의 열매를 순수열매와 종자로 분리하여 구분하지 않고, 종자(씨앗)이 분리되지 않은 종자를 포함하는 열매(이하, '종자열매'라 칭함)로부터 정유성분의 획득도 가능하다.However, it is also possible to obtain an essential oil component from a fruit (hereinafter referred to as "seed fruit") containing seeds in which the seeds are not separated, without distinguishing the fruit of the crow tree from pure fruit and seeds.
즉, 본 발명의 실시 예에서는 의약품, 식품 등의 다양한 조성물의 활용을 모색하기 위하여 순수열매와 종자를 분리하여 이로부터 정유성분을 획득하도록 한 것일 뿐, 종자열매(종자(씨앗)를 포함하는 열매)로부터 정유성분을 획득할 수 있으며, 이 또한 본 발명의 권리범주 안에 있다.That is, in the embodiment of the present invention, pure fruit and seed are separated from each other in order to search for utilization of various compositions such as medicines and foods, and only the essential oil component is obtained therefrom. The seed fruits (seeds ), Which is also within the scope of the present invention.
까마귀쪽나무의 열매(순수열매 또는 종자 또는 종자열매)로부터의 정유성분 추출 방법으로는 주정(EtOH)추출법, 초임계추출방법, 증류법, 압착법, 헥산추출법, 초고압추출법 등의 방법이 사용될 수 있다. EtOH extraction method, supercritical extraction method, distillation method, compression method, hexane extraction method, ultra high pressure extraction method and the like can be used as a method of extracting the essential oil component from the fruit of the crow tree (pure fruit or seed or seed fruit) .
상기 증류법과 압착법을 이용하는 경우 추출되는 정유성분의 양이 적은 단점을 가지고 있으며, 헥산을 이용한 추출법인 헥산추출법은 다른 추출방법보다 정유성분 추출 측면에서는 우수하며, 주정추출로부터 단계적인 처리를 통하여 추출하는 주정추출법 또한 우수한 장점을 가진다.The distillation method and the pressing method have a disadvantage in that the amount of the essential oil component to be extracted is small. The hexane extraction method, which is an extraction method using hexane, is superior to other extraction methods in terms of extraction of essential oil components. The alcohol extraction method also has excellent advantages.
- 주정을 이용한 단계적 추출방법 1(까마귀쪽나무 순수열매의 정유성분)- Stepwise extraction method using alcohol 1 (Essential oil component of pure fruit of crow tree)
건조된 까마귀쪽나무 순수열매에 용매인 주정 95%로 추출한 다음, 알코올을 제거하고 합성흡착제인 HP-20 레진을 이용하여 정유성분을 수득한다(까마귀쪽나무 순수열매 100g을 사용하였을 경우 15g의 정유성분을 수득하였다).The extract was extracted with 95% alcohol as a solvent in pure dried fruit of dried crow tree, then alcohol was removed and an essential oil component was obtained by using HP-20 resin, which is a synthetic adsorbent (15 g refined oil Lt; / RTI >
- 주정을 이용한 단계적 추출방법 2(까마귀쪽나무 순수열매의 정유성분)- Stepwise Extraction Method 2 using alcohol (Essential oil component of pure fruit of crow tree)
건조된 까마귀쪽나무 순수열매에 용매인 주정 95%로 추출한 다음, 알코올을 제거하고, 물을 넣어 층 분리 등의 단계적인 공정을 포함하여 정유성분을 수득한다(까마귀쪽나무 순수열매 100g을 사용하였을 경우 17g의 정유성분을 수득하였다).The crude oil was extracted with 95% alcohol as a solvent in pure dried fruit of dried crow tree, followed by stepwise processes such as alcohol removal and water separation to obtain an essential oil component (100 g pure fruit of a crow tree was used 17 g of essential oil component was obtained.
- - 헥산을Hexane 이용한 추출방법(까마귀쪽나무 순수열매의 정유성분) Extraction Method (Essential Oil Component of Pure Rice in Raven)
건조된 까마귀쪽나무 순수열매에 용매인 노말헥산(n-hexane)을 중량대비 9~11배수(바람직하게는 10배수)를 투입한 다음 상온(15~25℃)에서 24시간 동안 교반하여 추출한 다음 헥산(hexane) 층을 제거하여 까마귀쪽나무 열매 정유성분을 수득한다.N-hexane, which is a solvent, is added to the pure fruit of the dry crow's side tree and then extracted with stirring at room temperature (15 to 25 ° C) for 24 hours after 9 to 11 times (preferably 10 times) The hexane layer is removed to obtain the essential oil component of the crow's side.
- - 헥산을Hexane 이용한 추출방법(까마귀쪽나무 종자의 정유성분) Extraction Method (Essential Oil Component of Raven Seeds)
건조된 까마귀쪽나무(Litsea japonica) 종자에 용매 노말헥산(n-hexane)을 중량대비 9~11배수(바람직하게는 10배수)를 투입하여 상온(15~25℃)에서 24시간 동안 교반하여 추출한 다음 헥산(hexane) 층을 제거하여 까마귀쪽나무 종자 정유성분을 수(획)득한다.The seeds of Litsea japonica were seeded with dried n-hexane in an amount of 9 to 11 times (preferably 10 times) by weight and stirred at room temperature (15 to 25 ° C) for 24 hours. The next hexane layer is removed to obtain the essential oil component of the crow tree.
획득되는 상기 까마귀쪽나무 순수열매 정유성분과 종자 정유성분은 각각 단독으로 사용할 수 있으며, 또는 일정비율로 혼합하여 사용할 수도 있는 것이다.
The crude essential oil component and the seed essential oil component of the corn root obtained from the crow can be used singly or may be mixed at a certain ratio.
본 발명의 까마귀쪽나무 열매로부터 획득되는 순수열매(종자제외)와 종자(열매의 씨앗)의 정유성분은 DPPH radical scavenging activity, 총 페놀함량측정 등을 통하여 항산화효과를 확인하였다. The antioxidative effects of essential oils (seeds) and seeds (seeds of fruits) obtained from crow's nuts of the present invention were confirmed by DPPH radical scavenging activity and total phenol content measurement.
따라서 본 발명에 따른 까마귀쪽나무 열매로부터 획득되는 순수열매와 종자의 정유성분은 항산화효과에 유용한 식품 및 건강기능식품 또는 의약품의 조성물원료로 사용될 수 있다. Therefore, the pure fruit obtained from the crow's nuts according to the present invention and the essential oil component of the seeds can be used as a raw material for food, health functional food or pharmaceutical composition useful for antioxidative effect.
본 발명의 항산화조성물은 까마귀쪽나무 열매로부터 획득되는 순수열매와 종자의 정유성분과 함께 항산화효과를 갖는 공지의 유효성분을 1종 이상 함유할 수 있다.The antioxidant composition of the present invention may contain one or more known active ingredients having an antioxidative effect together with the pure fruit obtained from the corn root fruit and the essential oil component of the seed.
본 발명의 항산화조성물은 항산화효과를 목적으로 건강기능식품에 첨가되어 사용될 수도 있다. The antioxidant composition of the present invention may be added to a health functional food for the purpose of antioxidant effect.
상기 까마귀쪽나무 순수열매 또는 종자의 정유성분을 식품 첨가물로 사용할 경우, 상기 까마귀쪽나무 순수열매 또는 종자의 정유성분을 그대로 첨가하거나 또는 이의 분획물을 사용하거나, 다른 식품 또는 식품 성분과 함께 사용할 수 있고, 통상적인 방법에 따라 적절하게 사용할 수 있다.
When pure white fruit of the crow tree or essential oil component of the seed is used as a food additive, the essential oil component of the pure white fruit or seed of the crow can be added as it is, or its fractions can be used, or it can be used together with other foods or food ingredients , And can be appropriately used according to a conventional method.
이하, 본 발명의 이해를 돕기 위하여 바람직한 실시 예를 제시한다. 그러나 하기의 실시 예는 본 발명을 쉽게 이해하기 위하여 제공되는 것일 뿐, 실시 예에 의해 본 발명의 내용이 한정되는 것은 아니다.
Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easy understanding of the present invention, and the present invention is not limited by the examples.
실시 예 1Example 1 : 까마귀쪽나무 순수열매의 정유성분 제조 : Manufacture of Essential Oils of Pure Fruit of Crow Tree
동결건조된 까마귀쪽나무(Litsea japonica) 순수열매 500g에 95% 주정 5000㎖를 첨가하여 상온(20℃)에서 24시간 동안 교반추출 한 다음 알코올을 제거하고, 물을 넣은 첨가하는 공정을 단계적으로 추진하여 까마귀쪽나무 순수열매의 정유성분 85g을 수득하였다.Lyssea japonica was prepared by adding 5000 ml of 95% alcohol to 500 g of pure fruit of Lysoa japonica, stirring it at room temperature (20 ° C) for 24 hours, removing alcohol, and gradually adding water To obtain 85 g of an essential oil component of pure fruit of a crow tree.
실시 예 2Example 2 : 까마귀쪽나무 순수열매의 정유성분 제조 : Manufacture of Essential Oils of Pure Fruit of Crow Tree
동결건조된 까마귀쪽나무(Litsea japonica) 순수열매 500g에 노말헥산(n-hexane) 5000㎖를 첨가하여 상온(20℃)에서 24시간 동안 교반추출 한 다음 헥산을 제거하여 까마귀쪽나무 순수열매의 정유성분 95g을 수득하였다.5,000 ml of n-hexane was added to 500 g of lysoya japonica pure lychee, and the mixture was stirred at room temperature (20 ° C.) for 24 hours. Then, hexane was removed to obtain pure oil 95 g of a component were obtained.
실시 예 3Example 3 : 까마귀쪽나무 종자의 정유성분 제조 : Manufacture of Essential Oil Components of Crow Tree
동결건조된 까마귀쪽나무(Litsea japonica) 종자 500g에 노말헥산(n-hexane) 5000㎖를 첨가하여 상온(20℃)에서 24시간 동안 교반추출 한 다음 헥산을 제거하여 까마귀쪽나무 종자의 정유성분 46g을 수득하였다.5000 ml of n-hexane was added to 500 g of lysoya japonica seeds, and the mixture was stirred at room temperature (20 ° C) for 24 hours. Then, hexane was removed to obtain 46 g of an essential oil component ≪ / RTI >
실시 예 4Example 4 : 까마귀쪽나무 순수열매의 정유성분 제조 : Manufacture of Essential Oils of Pure Fruit of Crow Tree
동결건조된 까마귀쪽나무(Litsea japonica) 순수열매 500g에 95% 주정 5000㎖를 첨가하여 상온(20℃)에서 24시간 동안 교반추출 한 다음 HP-20 레진을 이용하여 까마귀쪽나무 순수열매의 정유성분 75g을 수득하였다.
Lyssea japonica was prepared by adding 5000 ml of 95% alcohol to 500 g of pure fruit of Lysoa japonica and stirring for 24 hours at room temperature (20 ° C). Then, using HP-20 resin, 75 g.
실험 예 1Experimental Example 1 : 까마귀쪽나무 순수열매 정유성분의 성분분석 : Analysis of Components of Essential Oil Components of Pure Fruit of Raven
상기 실시 예1과 실시 예2에 따라 얻어진 까마귀쪽나무 순수열매 정유성분의 분석실험을 수행하였다.Analysis of the essential oil component of pure hull wood obtained according to Example 1 and Example 2 was carried out.
실험방법 : 기기는 Shimadzu, QP2010Plus를 사용하였으며, Column은 RTX-5(30m x 0.25mm, 0.25㎛)을 사용하였고, 기타조건으로는 Injection 온도 250℃, Detector 온도 250℃, Mass range(m/z) 30-400, Injection volume 1μL으로 분석하였다. Experimental method: Instrument Shimadzu, it was used QP2010Plus, Column is RTX-5 (30m x 0.25mm, 0.25㎛) a was used, with other conditions Injection temperature 250 ℃, Detector temperature 250 ℃, Mass range (m / z ) 30-400, and injection volume 1 μL.
실험결과 : 상기 실시 예 1과 실시 예2에 따라 얻어진 까마귀쪽나무 순수열매 정유성분을 분석하여 도 3와 도 4, 그리고 아래 표 1과 표 2와 같은 결과를 얻었다. Experimental Results: The components of essential oil of pure white corn obtained according to Example 1 and Example 2 were analyzed to obtain the results shown in FIG. 3 and FIG. 4, and Table 1 and Table 2, respectively.
실시 예1의 순수열매 정유성분은 Beta-cubebene(19.7±2.4%), Beta-selinene(12.1±3.1%), Beta- Caryophyllene(8.2±1.2%) 등을 함유되어 있었으며, The pure essential oil components of Example 1 contained Beta-cubebene (19.7 ± 2.4%), Beta-selinene (12.1 ± 3.1%) and Beta-Caryophyllene (8.2 ± 1.2%
실시 예2의 순수열매 정유성분은 Beta-cubebene(21.7±1.9%), Beta-selinene(11.1±1.8%), Beta- Caryophyllene(7.4±1.6%) 등을 함유되어 있었다.The pure fruit essential oil component of Example 2 contained Beta-cubebene (21.7 ± 1.9%), Beta-selinene (11.1 ± 1.8%) and Beta-Caryophyllene (7.4 ± 1.6%).
실험 예 2Experimental Example 2 : 본 발명에 따른 까마귀쪽나무 열매 또는 종자 정유성분의 항산화효과 측정(1) : 총 페놀함량측정 : Determination of antioxidative effect of essential oil component of corn root or seed according to the present invention (1): Total phenol content measurement
상기 실시 예 1부터 실시 예 4까지 얻어진 까마귀쪽나무 열매로부터 획득되는 정유성분의 항산화효과를 확인하기 위하여 총 페놀함량을 측정하였다.The total phenol content was measured to confirm the antioxidative effect of the essential oil component obtained from the corn root fruit obtained from Examples 1 to 4 above.
실험방법 : 총 페놀함량은 folin-ciocalteu's phenol reagent가 시료의 페놀성화합물에 의해 몰리브덴 청색으로 환원되는 원리로 측정하였다. 각 시료 1 mL에 2% follin-ciocalteau's phenol regent 1 mL 및 10% Na2CO3 용액을 1 mL을 첨가하여 혼합한 후 상온에서 1시간 동안 방치하였다. 그리고 상등액을 microplate reader(Molecular Devices, Sunnyvale, CA, USA)를 이용하여 750 nm에서 흡광도를 측정하였다. 표준물질은 gallic acid를 이용하였다. Test method: The total phenol content was measured by the principle that the phenol reagent of the folin-ciocalteu was reduced to molybdenum blue by the phenolic compound of the sample. 1 mL of 2% follin-ciocalteau's phenol regent and 1 mL of 10% Na 2 CO 3 solution were added to 1 mL of each sample, and the mixture was allowed to stand at room temperature for 1 hour. The supernatant was measured at 750 nm using a microplate reader (Molecular Devices, Sunnyvale, Calif., USA). Gallic acid was used as a reference material.
실험결과 : 상기 실시 예 1부터 실시 예 4에서 얻어진 까마귀쪽나무 열매 정유성분에 대하여 총 페놀함량을 측정하여 도 1과 [표 3]으로 나타내었다. 그 결과 까마귀쪽나무 열매 정유성분의 총 페놀함량이 66~75(mg GAE/100g)로 측정되었다. Experimental Results: Total phenol contents of the essential oil components of the crows obtained in Examples 1 to 4 were measured and shown in Table 1 and Table 3, respectively. As a result, the total phenolic content of the essential oil component of the crow's side was measured as 66 ~ 75 (mg GAE / 100g).
아래 [표 3]은 상기 실시 예 1부터 실시 예 4에 따라 얻어진 까마귀쪽나무 열매 정유성분의 총 페놀함량[Table 3] shows the total phenol content of essential oil components of corn root obtained according to Examples 1 to 4 above
(mg GAE/100g)Total phenolic content
(mg GAE / 100 g)
실험 예 3Experimental Example 3 : 본 발명에 따른 까마귀쪽나무 열매 또는 종자 정유성분의 항산화효과 측정(2) : : Determination of Antioxidative Activity of Essential Oils of Rice or Seed of Raven according to the Present Invention (2): DPPHDPPH radicalradical scavengingscavenging activityactivity
상기 실시 예 1부터 실시 예 4까지 얻어진 까마귀쪽나무 열매로부터 획득되는 정유성분의 항산화효과를 확인하기 위하여 DPPH radical scavenging activity를 측정하였다.DPPH radical scavenging activity was measured to confirm the antioxidative effect of essential oil components obtained from the corn root fruit obtained from Examples 1 to 4 above.
실험방법 : DPPH 라디칼 소거능 실험을 위하여 각각의 정유성분을 일정 농도로 사용하였다. 시료 0.2 mL에 ethanol로 용해한 0.4 mM DPPH 용액 0.8 mL을 첨가하여 혼합한 후 상온에서 10분간 반응하였다. 그리고 microplate reader를 사용하여 490 nm에서 흡광도 값을 측정한 후 다음 하기 수학식 1을 이용하여 결과 값을 나타내었다. Experimental method: For the DPPH radical scavenging activity, each essential oil component was used at a certain concentration. 0.8 mL of 0.4 mM DPPH solution dissolved in ethanol was added to 0.2 mL of the sample, and the mixture was reacted at room temperature for 10 minutes. Then, the absorbance was measured at 490 nm using a microplate reader, and the following results were obtained using the following equation (1).
[수학식 1][Equation 1]
DPPH radical scavenging activity(%) = (1-AExperiment/AControl)×100IL-1βDPPH radical scavenging activity (%) = (1-A Experiment / A Control ) x 100 IL-1?
실험결과 : 본 발명의 실시 예 1과 실시 예 4에 따라 얻어진 까마귀쪽나무 열매 정유성분과 종자 정유성분에 대하여 DPPH radical scavenging activity를 측정하여 도 2과 [표 4]로 나타내었다. 그 결과 까마귀쪽나무 열매 정유성분의 DPPH radical scavenging activity 56~58%로 측정되었다. Experimental Results: The DPPH radical scavenging activity was measured for the essential oil component and the seed essential oil component of the corn root obtained according to Example 1 and Example 4 of the present invention, and is shown in FIG. 2 and FIG. As a result, the DPPH radical scavenging activity of essential oil component of crow's side was measured to be 56 ~ 58%.
아래 [표 4]는 상기 실시 예 1과 실시 예 2에 따라 얻어진 까마귀쪽나무 열매로부터 획득된 정유성분의 DPPH radical scavenging activityTable 4 below shows the DPPH radical scavenging activity of the essential oil components obtained from the corn root fruit obtained according to Example 1 and Example 2 above
activity(%)DPPH radical scavenging
activity (%)
본 발명의 까마귀쪽나무의 열매로부터 획득되는 순수열매 정유성분과 종자 정유성분은 항산화효과를 가진다.The pure fruit essential oil component and the seed essential oil component obtained from the fruit of the crow tree of the present invention have an antioxidative effect.
따라서 본 발명에 따른 까마귀쪽나무 열매로부터 획득되는 정유성분은 항산화효과에 유용하게 사용될 수 있을 것이다.Therefore, the essential oil component obtained from the crow's nuts according to the present invention may be useful for the antioxidative effect.
Claims (3)
상기 정유성분은 까마귀쪽나무의 순수열매 또는 종자열매 또는 종자 중 어느 하나로부터 획득한 것임을 특징으로 하는 황산화조성물.The method according to claim 1,
Wherein the essential oil component is obtained from pure fruit or seed fruit or seed of a crow tree.
상기 정유성분은 까마귀쪽나무의 순수열매로 획득되는 순수열매 정유성분과, 까마귀쪽나무의 종자로 획득되는 종자 정유성분이며,
상기 순수열매 정유성분과 종자 정유성분은 단독 또는 혼합사용됨을 특징으로 하는 황산화조성물.The method according to claim 1,
The essential oil component is a pure essential oil component obtained by pure fruit of a crow tree and a seed essential oil component obtained by a seed of a crow tree,
Wherein the purified essential oil component and the seed essential oil component are used alone or in combination.
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| KR20140019280A (en) * | 2010-07-26 | 2014-02-14 | 노이로툰 아게 | Process for the preparation of dimiracetam |
| CN115420832A (en) * | 2022-09-20 | 2022-12-02 | 贵州医科大学 | GC-MS fingerprint spectrum and multi-index content determination method of litsea pungens oil |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20140019280A (en) * | 2010-07-26 | 2014-02-14 | 노이로툰 아게 | Process for the preparation of dimiracetam |
| CN115420832A (en) * | 2022-09-20 | 2022-12-02 | 贵州医科大学 | GC-MS fingerprint spectrum and multi-index content determination method of litsea pungens oil |
| CN115420832B (en) * | 2022-09-20 | 2024-03-19 | 贵州医科大学 | GC-MS fingerprint spectrum and multi-index content determination method for litsea coreana oil |
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