KR20140136126A - Food and pharmaceutical composition for preventing or improving constipation comprising extracts or powder of Eisenia bicyclis as effective component - Google Patents

Abstract

The present invention relates to a food composition and a pharmaceutical composition containing an Eisenia bicyclis extract or an Eisenia bicyclis powder as an active ingredient to prevent or alleviate constipation. By investigating that the Eisenia bicyclis extract or Eisenia bicyclis powder is excellent to promote enterokinesia, intestinal regulation, and alleviate constipation in a mouse, the present invention is useful to treat and prevent various enteric diseases such as constipation, and the Eisenia bicyclis extract or Eisenia bicyclis powder can be useful as a food or pharmaceutical composition to prevent, alleviate, or treat constipation.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a food composition and a pharmaceutical composition for preventing or improving constipation, which comprises rhubarb extract or rhubarb powder as an active ingredient,

The present invention relates to a food composition and a pharmaceutical composition for preventing or ameliorating constipation, which contain rhubarb extract or rhubarb powder as an active ingredient. According to the present invention, the rhubarb extract or the rhubarb powder can be used as a composition for promoting intestinal motility, And is useful for the treatment and prevention of various intestinal diseases such as constipation. Thus, it can be effectively used as a food or pharmaceutical composition for preventing, ameliorating or treating constipation.

As the industry is getting more sophisticated, the aging of the population is accelerating, and the automobile culture is getting more and more widespread, the number of constipation patients is getting bigger because of the physical lack of exercise.

Constipation means that the passage of waste through the lower part of the large intestine is delayed, resulting in the release of a stiff stool with low water content into the anus. Symptoms of constipation patients with constipation include fullness, rectal pressure, and frequent need for excretion. In the case of chronic constipation, patients with chronic constipation may experience bloating, trim, nausea, reflux, and abdominal discomfort. If chronic constipation is not treated, patients with chronic constipation may experience headache, dizziness, loss of appetite, . However, most constipated patients feel shame or constipation treatment itself is very troublesome. Furthermore, patients with constipation often do not promptly treat constipation because most constipation patients do not consider constipation itself to be a serious disease. As a method for treating constipation, it is desirable to regain regular regular meals and regular bowel habits, or to increase the intake of moisture and fiber-rich foods and water, such as fresh fruits and vegetables and uncooked cereals.

On the other hand, rhubarb is a seaweed plant that is a special product of Ulleungdo and Dokdo. It is used for edible use and also used as seaweed substitute. In addition, since rhubarb is used as a raw material of alginic acid and contains a large amount of minerals such as iodine and potassium, rhubarb has been used as a material for constipation of local people in Ulleung. The efficacy of rhubarb is very cold, the taste is good, and there is no poison, so it is effective in preventing the ejaculation and menstruation. It is effective for constipation (constipation constipation), fever constipation .

Korean Patent Publication No. 0344325 discloses a tea having an effect of improving constipation symptoms and a method for producing the same, Korean Patent Publication No. 2007-0082296 discloses an oriental health supplement food effective for constipation treatment, There has been no report on food compositions and pharmaceutical compositions for preventing or improving constipation containing rhubarb extract or rhubarb powder as an effective ingredient.

The present invention has been made in view of the above-mentioned needs, and it is an object of the present invention to increase the number of feces by promoting peristalsis of the stomach in a mouse fed with an experimental diet containing rhubarb extract or rhubarb powder, Confirming that the feces weight is reduced, the excretion time is reduced, and the bowel movements are smoothed, confirming that the rhubarb extract or the rhubarb powder of the present invention is effective for improvement, prevention and treatment of constipation, thereby completing the present invention.

In order to solve the above problems, the present invention provides a food composition for preventing or improving constipation, which contains rhubarb extract or rhubarb powder as an active ingredient.

The present invention also provides a pharmaceutical composition for preventing or treating constipation, which contains rhubarb extract or rhubarb powder as an active ingredient.

Through the present invention, it has been confirmed that the rhubarb extract or rhubarb powder is excellent in the effect of promoting intestinal motility, dressing effect and constipation improvement in mice and is useful for the treatment and prevention of various intestinal diseases such as constipation, Or a pharmaceutical composition.

Fig. 1 is a schematic view showing a method for producing the rhubarb extract of the present invention.
Fig. 2 shows an experimental method in which an experimental diet containing the rhubarb extract of the present invention was administered to a mouse to examine the constipation-improving effect.
Fig. 3 shows changes in the number of feces in rufferamide-induced constipation mice according to the treatment with rhubarb extract and powder. (A) Changes in the number of fecal samples during the experiment (B) Changes in the number of feces before and after constipation and after the empirical formula (abbreviation: D (-1), before constipation; EE2, 1% of Rahalia ethanol extract, 1% of Rahul powder, 1% of Eulerian powder, 1% of Ganoderma lucidum powder, , Rhubarb powder 5%)
Fig. 4 shows changes in feces weight according to the treatment with luperamide-induced constipation mice and the treatment with rhubarb extract. (A) Fecal weight change during the experiment (B) Before and after constipation, after the empirical formula, changes in feces weight (D (-1), before constipation; EE2, 1% of the ethanol extract of rheumatoid, 1% of the ethanol extract of the rheumatoid, 1% of the rheumatoid powder, 2% of the rheumatoid ethanol extract, Powder 5%)
Fig. 5 shows changes in body weight of rufiramide-induced constipation mice following treatment with rhubarb extract and powder. (1%), EF (2%), EF (2%), EF (2%), EF Powder 5%)
Fig. 6 shows the measurement of intestinal mobility according to rufiramide-induced constipation mice treated with rhubarb extract and powder. (A) The intestinal motility of experimental animals, (B) The overall appearance of digestive tract of experimental animals. (1%), EF (2%), EF (2%), EF (2%), EF Powder 5%)
Fig. 7 shows the number of feces in the large intestine according to the treatment with rhubarb extract and powder in the luperamide-induced constipation mice. (1%), EF (2%), EF (2%), EF (2%), EF Powder 5%)

According to an aspect of the invention, the invention is rhubarb (Eisenia bicyclis extract or safflower powder as an active ingredient.

The rhubarb extract of the present invention may be extracted as water or an organic solvent or a mixed solvent thereof. The organic solvent is any conventional solvents which can be used, preferably water, C _{1 - 4} alcohol: may be a polar solvent or a mixture of these solvents, such as (for example methanol, ethanol, etc.). Preferably, the rhubarb extract may be ethanol or water extract, most preferably 95% ethanol or water extract, but is not limited thereto.

In addition, the rhubarb extract or the rhubarb powder of the present invention may be added to food or beverage for the purpose of preventing or improving constipation. At this time, the amount of the extract or powder in the food or beverage may be 0.01 to 15% by weight of the total food, and the health beverage composition may be added in a proportion of 0.02 to 5 g, preferably 0.3 to 1 g, Can be added.

The health functional beverage composition of the present invention is not particularly limited to the ingredients other than those containing the extract or powder as an essential ingredient in the indicated ratios and may contain various flavors or natural carbohydrates as additional ingredients such as ordinary beverages have. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors (tautatin, stevia extract, for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above . The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above, the extract or powder of the present invention may contain various kinds of nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and heavies (cheese, chocolate etc.), pectic acid and its salts, And salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the extract or powder of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks. These ingredients may be used independently or in combination, and the proportion of such additives is not so critical, but is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the extract or powder of the present invention.

The present invention also provides a pharmaceutical composition for preventing or treating constipation, which contains rhubarb extract or rhubarb powder as an active ingredient.

In the pharmaceutical composition according to one embodiment of the present invention, the extract is as described in the above food composition.

The pharmaceutical composition for preventing or treating constipation according to the present invention may contain 0.02 to 80% by weight, preferably 0.02 to 50% by weight, of the extract or the powder, based on the total weight of the composition.

The pharmaceutical composition comprising the extract or powder of the present invention may further comprise suitable carriers, excipients and diluents conventionally used in the preparation of pharmaceutical compositions.

The pharmaceutical dosage forms of the extracts or powders of the present invention may be used in the form of their pharmaceutically acceptable salts, and may be used alone or in combination with other pharmaceutically active compounds as well as in suitable aggregates.

The pharmaceutical composition containing the extract or powder according to the present invention can be administered orally or rectally in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups and aerosols, external preparations, suppositories and sterilized injection solutions Can be used. Examples of the carrier, excipient and diluent which can be contained in the composition containing the extract or powder include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, Various compounds or mixtures including calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil . In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, oligosaccharides, sucrose, (sucrose), lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Examples of the liquid preparation for oral administration include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The preferred dosage of the extract or powder of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the type of drug, the route of administration and the period of time, but can be appropriately selected by those skilled in the art. However, for the desired effect, the extract or powder of the present invention is preferably administered at 0.0001 to 100 mg / kg, preferably 0.001 to 100 mg / kg per day. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

The extract or powder of the present invention can be administered to mammals such as rats, mice, livestock, and humans in various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine or intracerebroventricular injections.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Experimental Method

Rhubarb  Extraction method

The extracts of the rhubarb extracts were obtained by repeating the procedure twice by adding 10% of 95% ethanol to rhubarb and stirring the mixture for 24 hours (150 rpm) and centrifuging to recover the supernatant. The ethanol extract was obtained by concentration under reduced pressure. After completion of the reaction, distilled water was added thereto, and the mixture was stirred at 150 rpm for 24 hours. After centrifugation, the supernatant was recovered by repeating the procedure twice to obtain a water extract, which was used as a sample after lyophilization (FIG. In addition, the rhubarb powder was washed with rhubarb to remove salt, and dried by a hot-air drying or freeze-drying method commonly used in the art, and used as a sample after pulverization.

General component

The rhubarb was analyzed according to the AOAC method. Moisture was measured by the atmospheric pressure drying method at 105 ℃, ash was used for ash extraction, Soxhlet extraction was used for lipid, Kjeldahl method was used for protein, and AOAC method was used for crude fiber modified Henneberg-Stohmann method. Sugar content was determined by subtracting water, protein, lipid, crude fiber and ash content from 100g.

Rhubarb  Analysis of nutritional and physiological active ingredients

Free amino acids, proteins

Spectral analysis using Lowry method was used for protein content analysis. Pretreatment for amino acid analysis was performed by dissolving 0.2 g of the powdered sample in 5 ml of 6N HCl at 125 ° C for 14 hours, neutralizing with 5 ml of 6N NaOH, and filtering with a 0.45 μm membrane filter to prepare a sample solution. AQC reagent was prepared by dissolving 6-aminoquinolyl-N-hydroxysuccinimidyl carbonate in 1 mL of AQC reagent diluent. Amino acid standard solution and 20 μl of each test solution were added to each reaction tube, and 140 μl of AQC 0.2M borate buffer was added. After reacting for 10 seconds, 40 μl of AQC reagent was added, and the mixture was sealed for 2 minutes. Reacted and then cooled to prepare an analytical sample. 5 μl of this analytical sample was injected into HPLC and detected with a fluorescence detector while sequentially flowing solution A (acetate / phosphate buffer, pH 5.02) and solution B (60% acetonitrile) Respectively.

Total sugar and reducing sugar

The total sugar change was measured by the phenol sulfate method. The reagents used were concentrated sulfuric acid and 5% phenol. In the experiment, 500 μl of sample, 500 μl of 5% phenol and 2.5 ml of concentrated sulfuric acid were added to the tube, and the mixture was incubated for 10 minutes in boiling water. After cooling for 15 minutes in ice, the mixture was analyzed with a spectrophotometer (UV / VIS spectrometer, Jasco, Hachioji, Japan) was used to measure the absorbance at 490 nm. The standards were quantified using sucrose.

In order to measure the change of reducing sugar, the DNS method was applied. The reagent was prepared by mixing the DNS reagent (1% of dinitrosalic acid reagent solution, 10 g of dinitrosalic acid, 2 g of phenol (optional, see Note 1), 0.5 g of sodium sulfite, 10 g of LOCKID, and 1 liter of water), and 40% of a potassium sodium tartrate solution was used. First, 1 ml of the sample and 3 ml of the DNS were added to the tube, 40% potassium sodium tartrate solution was added, and the mixture was reacted for 5 minutes with boiling water. The reaction solution was taken out of the tube, quenched by ice and subjected to spectrophotometer (UV / VIS spectrometer , Jasco, Hachioji, Japan) at 575 nm. The standard substance was quantified using glucose (Dextrin).

Free sugar analysis

Analysis of free sugars was performed using HPLC. First, the sample was filtered with a membrane filter (0.45um) and used as an analytical sample. The used column was an LC-20A Prominence HPLC system (SHIMADZU, Japan) equipped with a Shodex SUGAR KS-801 column The column temperature was maintained at 80 ° C, the mobile phase was H ₂ O, and the solvent flow rate was 0.7 ml / min for 40 minutes. The detector was measured with RID-10A (SHIMADZU, Japan), and Nystose, Kestose, Sucrose, Glucose, Fructose (Sigma, USA) Were used for the determination of free sugars.

Total polyphenolic component analysis

0.1 g of the dried sample was extracted three times with 20 ml of a 75% methanol solution using the Folin-Denis method, and the extract was mixed and concentrated under reduced pressure at 40 ° C. Then, 5 ml of distilled water and 2 ml of 10% Folin-Cioalteus were added to 1 ml of the sample extract solution prepared by diluting with 20 ml of a 75% methanol solution, and left for 3 minutes. ₂ ml of a 7.5% Na ₂ CO ₃ solution was added thereto, diluted with distilled water, adjusted to 20 ml, allowed to stand at room temperature for 1 hour, and absorbance was measured at 760 nm. A standard curve was prepared using tannic acid as a standard product, and the total phenol content was determined. Qualitative and quantitative analysis of extracted polyphenolic materials was compared with standard products using HPLC.

Total flavonoid substance content measurement

Total flavonoid content was determined by adding 10 ml of diethylene glycol and 1 ml of 1N NaOH to 1 ml of sample and incubating at 37 ° C for 1 hour. The absorbance was measured at 420 nm. Total flavonoid content was calculated using a standard curve using naringin (0.5 mg / ml) as a standard.

Rhubarb  Constipation improvement efficacy

(A) Experimental methods and laboratory animals

An experimental method for analyzing the constipation-improving effect of the rhubarb extract of the present invention is shown in FIG. Sixty male ICR male rats, 5 weeks old, were purchased from Orient Bio and cultured for 7 days in a controlled environment (temperature 20 ± 2 ° C, humidity 55 ± 15%, 12 hrs / It was watered and adapted to the environment. Control group (C) (2) Negative control group (NC) (3) Control group (3) Control group (3) Control group ) 1% (GF1) (1) (5) GF2 (5) Rhubarb extract 0.5% (EE1) (6) Rhubarb extract 1% (EE2) ) And rhubarb powder 5% (EB2). Experimental diets were fed for one week, and diets and water were consumed without restriction during the entire period of the experiment.

(B) The empirical formula

The experimental diet was based on the AIN-76 diet. Based on the composition shown in Table 1, the experimental groups were customized to the pellet manufacturer, Gly-Bio, so that the content of each nutrient was the same. The dietary fiber content of the experimental diets was calculated by using the recommended dietary fiber intake (12g / 1000kcal) as shown in the Korean Nutrition Intake Standard (2005), so that the experimental formula contained about 5g of dietary fiber per 100g (435kcal) And about 5%. The rhubarb used in the present invention was collected in the offshore waters of Ulleungdo Island and was used for pelletization after grinding by hot air drying.

As a positive control, green vitreous fiber, which is a health functional food which is a commercially available bowel action product, was prepared. It was composed of 100% of powdery mildew and was made into pellets in the composition shown in Table 1. For the control diet, AIN-76A was used as the basic composition.

(C) induction of constipation state

Constipation was induced in the first 4 days by oral administration of looperamide (Looperamide, 5 mg / kg body weight suspended in 0.9% NaCl) twice daily (10:00, 17:00) Later, the treament diet caused constipation during the experiment as well as feeding. Luperamide, a constipation-inducing substance, was purchased from SIGMA-ALDRICH.

(D) Fecal weight measurement

Fecal weight was collected during 24 hours during the entire breeding period at a given time and weighed and weighed.

(E) GIT measurement test

Experimental animals were sacrificed for more than 16 hours and 0.1 ml of carmin (8 mg suspended in 1 ml of 0.5% Carboxymethylcellulose) was orally administered as a marker using Carmine dye. After oral administration, the mice were sacrificed 1 hour later, and the abdomen of the mouse was incised along the midline and excised from the pyloric portion to the rectum so as not to cut the intestines. The extracted digestive tract was dissected to confirm the best end of the carmine pigment. The length of the digested gut was measured so as to be easy to measure and the distance from the pylorus to the tip of the carmine pigment was measured. And the distance from the pylorus to the rectum (total length of digestive tract). The total length of the digestive tract (A) and the distance of travel to the best end of the carmine pigment (B) were calculated using the following formula to determine the carotene migration rate (T) of the administered carmine pigment.

(T) = (B / A) x 100 (%)

(F) Blood collection and sacrifice

In the case of blood collection, no anesthesia was the principle, and blood was collected using the orbital vein total blood collection method. During the collection, the tip of the pipette was pushed into the eye and downward using a pasteur. At this time, if you remove the pipette, the blood will follow. Blood was collected at over 500 μl and centrifuged at 3000 rpm for 20 minutes to separate plasma and stored frozen at -80 ° C until analysis. Blood was collected and then cervical dislocations were sacrificed.

(G) Number of feces in colon

After sacrifice, the entire digestive tract was removed and the number of feces contained in the large intestine was counted.

Example  One. Rhubarb  General ingredients and nutrients, Extraction yield  analysis

Moisture content, crude protein, crude protein and crude fat content of rhubarb powder were measured and shown in Table 2. Moisture content of rhubarb powder was 4.72%, ash content was 2.15%, crude protein content was 11.54% and crude fat content was 28.05%.

The extraction yield of rhubarb extract is 9.0% and the extraction yield of ethanol extract is 9.6%. As a result of nutritional and efficacy analysis, the total content of diaphora extract was 17.93 mg / g per 1 g of extract, higher than the total content of ethanol extract of 4.18 mg / g, and the polyphenol content was as high as 15.88 mg / g of ethanol extract Table 3).

Example  2. Experimental animals Number of feces  change

In the constipation-induced D (-1), there was no difference in the fecundity between the groups of experimental animals. However, in the D (0) causing constipation, the number of fecals was decreased in the other experimental groups except for the constipation-induced D (-1) (Fig. 3A). It was confirmed that constipation of laboratory animals was induced through the use of luperamide, which is a constipation inducing substance. Luparamide has been reported to cause constipation by lowering gastrointestinal motility as a laxative component. The number of fecal samples was increased as compared with the NC group that did not receive the experimental diet in D (1), and the experimental diet was fed in the experimental group at 1% of the rhubarb extract and 5% of the rhubarb powder at the second day And the number of feces in normal group was increased by D (2).

In the experimental group of rhubarb extract and rhubarb powder, the number of fecal samples was higher than that of NC group during the whole experimental period. In experimental group D (13), the group receiving 5% rhubarb powder showed more than twice the number of fecal samples I could. It was found that the intake of rhubarb relieves constipation. It was confirmed that rhubarb stimulates the peristalsis of the stomach by increasing the number of feces.

When constipation was induced, the number of the final fecal samples after the constipation induction (FIG. 3B) was not different before the constipation induction, and when the constipation was induced, the number of feces was 50% And the number of fecal samples after 15 days of experimentation showed that the number of fecal samples was increased in a concentration - dependent manner in the case of the rhubarb powder, and the number of fecal samples was higher than that in the control. The content of green hiba was 100% in the case of cardiovascular disease, and improvement of bowel activity and decrease of blood cholesterol were shown.

Example  3. Experimental animals Fecal weight  change

In the D (-1) group, the fecal weight of the experimental animals was about 0.40 g. In the D (0) group that caused the constipation, the fecal weight of the other groups except the control group Was less than 0.2 g, which was reduced by about 50% or more compared to that before inducing constipation (Fig. 4). This confirms that lupeamide causes constipation. After feeding the experimental diets, 1% of the rheumatoid extracts showed the same amount of feces as in the control group (control group) in D (1), which was the first day of feeding, and the other groups also caused constipation And the fecal amount was increased compared to the NC group which did not fed the experimental diets. The appearance of the fecal appearance of the fecal NC group of FIG. 4 was more color-coded and smaller than that of the fecal group of the control group. Also, in the case of the rhubarb extract and the rhubarb powder, the fecal appearance of the high concentration group was thicker and the feces size was larger than that of the low concentration group. This can be attributed to the dietary fiber of rhubarb. Dietary fiber is divided into insoluble dietary fiber and water-soluble dietary fiber. Insoluble dietary fiber has little affinity with water and does not easily ferment by intestinal microorganisms. Therefore, it stimulates intestine by increasing amount of stomach, To reduce the time of excretion, and smooth the bowel movements. In the case of water soluble dietary fiber, the amount of fecal matter was increased and the size and water content of feces were increased because of easy adsorption and swelling of water in the field.

Example  4. Experimental animal Dietary intake  change

The changes in dietary intake of experimental animals were divided into periods of constipation induction, constipation induction, and empirical formula. There was no significant difference in dietary intake between the groups before the induction of constipation. The duration of constipation was also lower in the NC group than in the control group, but there was no statistically significant difference. This confirms that a reduction in dietary intake does not reduce the amount of fecal matter, indicating that constipation has been induced through the use of luperamide.

The dietary intake of diabetic rats was significantly lower than that of diabetic rats. The dietary intake of diabetic rats was significantly higher than that of diabetic rats. , The daily intake was 5.37 g, which was smaller than the daily intake of 5.80 g of the NC group. This is not an increase in the fecal incidence with increasing dietary intake but an increase in the fecal incidence according to the empirical formula.

Example  5. Weight change in experimental animals

Fig. 4B shows the result of measuring the weight of the experimental animal before and after the induction of constipation. The body weights of the control group and the constipation-induced group were compared after the constipation induction, but the weight of the other groups except the NC group was not significantly different from the control group And the NC group had a difference from the control group. This shows that there is no special interaction between body weight and fecal matter since it does not show any particular tendency. In other words, it means that there is not much fecal volume of laboratory animals that are overweight.

In the control group, body weight was increased after the empirical formula compared to before the constipation induction. This is an increase in body weight over time. In the NC group, constipation was caused by constipation compared with before constipation, and the body weight was continuously decreased because of the decrease in dietary intake due to constipation. In the other experimental groups, there was no significant difference between before and after constipation induction, but in 5% of rhubarb compared to before constipation induction.

Example  6. Laboratory animals Travel rate  Measure

After the sacrifice, the carin pigment was orally administered, and the whole digestive tract was sacrificed 40 minutes after the administration, and the best end distance of the pigment was measured to measure the digestive tract migration rate as shown in FIG. In the control group, the digestive tract migration rate of the control group was about 85%, and the NC group had about 49% of the migration rate of the digestive tract. As a result, it was confirmed that the migration rate of the digestive tract was decreased. This indicated that luperamide induced constipation and slowed the peristalsis of the intestines.

After 15 days of feeding the experimental diets after inducing constipation in the experimental animals, the rate of intestinal motility was increased in the experimental group compared to the NC group. This is due to insoluble dietary fiber of rhubarb. Insoluble dietary fiber has low affinity for water and is not easily fermented by intestinal microorganisms. Therefore, it stimulates the intestine by increasing the amount of the stomach, which helps the interlocking action and increases the intestinal motility. Therefore, it was concluded that rhubarb extracts and powders can improve constipation by increasing intestinal motility.

Example  7. Long-term weight change of experimental animals

The organ weights of the experimental animals are shown in Table 5. The heart weights of the experimental animals were not significantly different in the experimental groups. This indicates that the experimental diet of the sample is not toxic, and there is a statistically significant difference between the groups in the kidney weight and liver weight. However, since the control group, the NC group, and the sample empirical formula do not show a uniform tendency between the groups, Kidney weight, liver weight gain, and decrease. However, the cecum weight of the experimental animals showed that the cecum weight of the NC group was decreased by the statistical significance difference compared to the control group.

Example  8. In the large intestine of experimental animals Number of feces

The number of feces in the intestine was measured after the whole digestive tract was sacrificed after the sacrifice of the experimental animals, and the results are shown in Fig. The reason for measuring the number of feces in the large intestine is to measure the stimulation of the peristalsis of the large intestine. Because the peristaltic movement of constipation is slowed, the movement of the feces is slowed and the feces staying in the large intestine increases. When the intestinal motility of the intestines is promoted, the feces to stay in the large intestine is reduced, and as the feces is smaller, the intestinal motility of the intestines is promoted. The number of fecal necrosis in the large intestine was found to be 9, which was significantly higher than that of the control group. This confirms that luperamide reduces the intestinal motility of the intestines and increases fecal stays in the colon.

Example  9. Blood triglyceride content and total cholesterol content of experimental animals

Table 6 shows the results of measurement of serum lipid content (TG) and total cholesterol content (TC) after sacrifice by feeding orally administered luperamide, which is a constipation inducing substance, to experimental animals. There was a report that the blood cholesterol level and triglyceride level were increased when the constipation persists. In this experiment, the constipation-inducing substance, luperamide, , But the concentration of triglyceride in the extracts of rhubarb and rhubarb was decreased in a concentration - dependent manner. It is believed that this is related to the anti-obesity effect of rhubarb, and the dietary fiber of Rhubarb reduces the content of triglycerides.

Claims (4)

Eisenia bicyclis ) extract or safflower powder as an active ingredient. The food composition according to claim 1, wherein the extract is ethanol or water extract. Eisenia bicyclis ) extract or a safflower powder as an active ingredient. The pharmaceutical composition according to claim 3, wherein the extract is ethanol or water extract.

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