KR20140116997A - A cell culture device and cell culture method using the same - Google Patents
A cell culture device and cell culture method using the same Download PDFInfo
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- KR20140116997A KR20140116997A KR1020130031495A KR20130031495A KR20140116997A KR 20140116997 A KR20140116997 A KR 20140116997A KR 1020130031495 A KR1020130031495 A KR 1020130031495A KR 20130031495 A KR20130031495 A KR 20130031495A KR 20140116997 A KR20140116997 A KR 20140116997A
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- cell
- cell culture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/16—Apparatus for enzymology or microbiology containing, or adapted to contain, solid media
- C12M1/18—Multiple fields or compartments
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
Abstract
Description
The present invention relates to a cell culture apparatus and a cell culture method using the same.
In recent years, tissue engineering or regenerative medicine, which treats cells and tissues by in vitro (out-of-body) cultures and then applies them to living bodies, has received attention. Particularly, it has been commercialized as a technology for culturing cells isolated from tissues such as the patient's own or donor's skin, oral mucosa, cornea, cartilage, fat, etc. and applying them to living bodies.
These cells have been transplanted into simple scaffolds or suspended forms, and attempts have been made to add their efficacy using biomaterials. However, such attempts have been difficult to apply for commercialization and mass production as a level of research that makes conditions for cell cultivation possible in biomaterials.
For example, when a cell is inoculated with a biocompatible material as a support on the bottom of a commercially available culture container, the cells that have been collected under the support are not cultured on the support. In order to prevent this, it is possible to cultivate the cell by sealing the edge of the support with an adhesive material such as silicone, but it is difficult to remove it for implantation.
Therefore, it is necessary to construct a culture container equipped with a culture facility that meets the size and specification of the support so that the cells can be cultured in large quantities using a biocompatible material as a support and directly applied to a human body.
The present invention relates to a cell culture apparatus which is easy to separate and use for a human body or for a specific purpose, and to provide a cell culture method using the cell culture apparatus.
The present invention relates to a container-shaped support support unit; A cell culture unit positioned above the supporter receiving unit and having upper and lower openings; And a cell culture apparatus.
In addition, the present invention provides a cell culture system comprising a plurality of cell culture apparatuses.
In addition, the present invention provides a method for preparing a cell culture, comprising the steps of (a) placing a polymer scaffold on a support of a support support and coating the cell scaffold with a cell adhesion polymer; And (b) attaching cells to the polymer scaffold after coating with a cell adhesive polymer; And a cell culture method.
The present invention also provides a method for producing a cell culture, comprising the steps of: (a) placing a polymer scaffold on a support of a support support and attaching cells; And (b) coating the cell-bound polymer scaffold with a cell adhesion polymer; And a cell culture method.
The present invention also relates to a method for producing a cell-adhesion polymer, comprising the steps of: (a) mixing a cell with a cell adhesion polymer to prepare a mixture; And (b) placing the polymer scaffold on the step of the support scaffold and attaching the scaffold to the polymer scaffold; And a cell culture method.
The present invention also provides a method for producing a cell culture, comprising the steps of: (a) placing a polymer scaffold on a support of a support support and attaching cells; (b) culturing the cells attached to the polymer scaffold; And a cell culture method.
The cell culture apparatus according to the present invention can be configured so that the support is suitable for cell culture and can easily be detached from the culture container so that the support can be applied to the human body. In addition, the biocompatible polymer scaffold and the cells cultured thereon can further improve the action in the human body.
1 is a view showing a cell culture apparatus according to the present invention.
2 is a view showing a cell culture system according to the present invention.
3 is a view showing a cell culture system according to the present invention.
The present invention relates to a container-shaped support support unit; A cell culture unit positioned above the supporter receiving unit and having upper and lower openings; To a cell culture apparatus.
In addition, a step is formed on the upper inner side surface of the supporter receiving part, and the supporter receiving part includes at least one fastening means for fastening the cell culture part to the upper surface.
And a tightening means for bringing the support support portion and the cell culture portion in close contact with each other over a part or the entire circumference thereof.
And a cover attached to an upper end of the cell culture unit to prevent infiltration of microorganisms.
The present invention relates to a cell culture system comprising a plurality of cell culture apparatuses.
The present invention relates to a container-shaped support support unit; A cell culture unit positioned above the supporter receiving unit and having upper and lower openings; And a cell culture apparatus.
In addition, the present invention provides a cell culture system comprising a plurality of cell culture apparatuses.
In addition, the present invention provides a method for preparing a cell culture, comprising the steps of (a) placing a polymer scaffold on a support of a support support and coating the cell scaffold with a cell adhesion polymer; And (b) attaching cells to the polymer scaffold after coating with a cell adhesive polymer; And a cell culture method. The cell culture method may further include (c) culturing the adhered cells.
The present invention also provides a method for producing a cell culture, comprising the steps of: (a) placing a polymer scaffold on a support of a support support and attaching cells; And (b) coating the cell-bound polymer scaffold with a cell adhesion polymer; And a cell culture method. Further, the cell culture method may further include the step of (c) culturing the cell on the polymer scaffold after coating with the cell adhesion polymer.
The present invention also relates to a method for producing a cell-adhesion polymer, comprising the steps of: (a) mixing a cell with a cell adhesion polymer to prepare a mixture; And (b) placing the polymer scaffold on the step of the support scaffold and attaching the scaffold to the polymer scaffold; And a cell culture method. The cell culture method may further include (c) culturing the cells after attaching the mixed solution to a polymer scaffold.
In addition, the present invention provides a method for preparing a cell culture, comprising the steps of: (a) placing a polymer scaffold on a support of a support support and attaching cells; (b) culturing the cells attached to the polymer scaffold; And a cell culture method. The cell culture method may further include (c) coating the polymer scaffold with a cell adhesion polymer.
The polymer scaffold may be a biocompatible polymer scaffold, a carcass skin polymer scaffold or a ampholyte polymer scaffold.
Particularly, the biocompatible polymer scaffold may be selected from the group consisting of polyvinyl alcohol (PVA), polyurethane (PU), polyethylene (PE), polyacrylic acid (PAA), polyoxyethylene (POE), polyethylene oxide (PEO), polytetrafluoroethylene (PES), polyvinyl chloride (PVC), polyvinylidene fluoride (PTFE), polypropylene (PP), polyethylene terephthalate (PET), polyamide (PA), polyacrylonitrile (PVDF), polysiloxane (Silicone Rubber), polyglycolic acid (PGA), polylactic acid (PLA), polymethacrylic acid (PMA), polyacrylamide (PAM), polysaccharide (PS), polyvinylpyrrolidone ), A biocompatible polymer comprising at least one member selected from the group consisting of silicone, alginic acid, sodium alginate, cellulose, pectin, chitin, chitosan, gelatin, collagen, fibrin, hyaluronic acid, silk fibroin, natural rubber, have.
The cells may be skin cells, stem cells, corneal cells, cartilage cells, hair follicular cells or mucosal cells.
The skin cells may be keratinocytes, melanocytes, endothelial cells, hair follicle stem cells or fibroblasts, and the stem cells may be embryonic stem cells , Induced pluripotent stem cells, or adult stem cells.
The cell adhesion polymer may be alginic acid, fibrin, gelatin, collagen or hyaluronic acid.
Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. The sizes and shapes of the components shown in the drawings may be exaggerated for clarity and convenience. In addition, terms defined in consideration of the configuration and operation of the present invention may be changed according to the intention or custom of the user, the operator. Definitions of these terms should be based on the content of this specification.
It is to be understood that within the scope of the appended claims, the invention may be practiced otherwise than as specifically described within the spirit and scope of the appended claims. Of course.
FIG. 1 is a view showing a cell culture apparatus according to the present invention, FIG. 2 is a view showing a cell culture system according to the present invention, and FIG. 3 is a diagram showing a cell culture system according to the present invention. Hereinafter, the cell culture apparatus of the present invention and the cell culture method using the same will be described in detail with reference to FIGS. 1 to 3 and Examples.
The present invention relates to a cell culture apparatus which is easy to use as a support for a cell cultured as described above, or to be separated and used for a specific purpose.
Herein, the support may be a biocompatible polymer scaffold, a carcass skin polymer scaffold or a amphipathic polymer scaffold. For example, the scaffold may be a skin scaffold, a stem cell, a corneal cell, a chondrocyte, The surface of the dressing material can provide a contact surface with the dressing material.
The present invention relates to a cell culture apparatus, and includes a support support unit and a cell culture unit, as shown in FIGS. 1 to 3.
More specifically, the supporter receiving portion has a container shape and a step is formed on the upper inner side surface. On the other hand, the step can support the support easily, and is for easily removing the support from the support receiving part.
In addition, the cell culture unit is located at the upper portion of the receiving unit, and the upper and lower portions are opened.
The support base of the present invention includes at least one fastening means. The fastening means is for fastening the cell culture section to the upper surface of the supporter receiving section, and any fastening means having such a purpose may be used.
At this time, it is possible to provide a tightening means for bringing the cell culture section into close contact with the supporter receiving section over a part or the entire periphery of the supporter receiving section and the cell culture section. The contact means may be an O-ring commonly used in the art.
In addition, the present invention may further include a cover which is bonded to the upper end of the cell culture unit to prevent microorganisms from infiltrating.
In addition, the cell culture apparatus of the present invention may be made of a light transmitting material through which light passes.
The present invention relates to a cell culture system characterized in that a plurality of cell culture apparatuses are formed as shown in FIG. 2 to FIG.
Depending on the purpose, the cell culture system can be varied in size, and mass culture may be possible.
The present invention relates to a cell culturing method using a cell culture apparatus or a cell culture system, and more particularly, to a cell culturing method using a cell culture apparatus or a cell culture system, comprising: (a) a step of placing a polymer scaffold on a support of a support support, And (b) attaching the cell to the polymer scaffold coated with the cell adhesive polymer; The present invention also relates to a method of culturing cells comprising culturing the adhered cells.
The present invention also provides a method for producing a cell culture, comprising the steps of: (a) placing a polymer scaffold on a support of a support support and attaching cells; And (b) coating the cell-bound polymer scaffold with a cell adhesion polymer; The method of the present invention may further include a step of culturing the cell after coating with the cell adhesion polymer.
The present invention also relates to a method for producing a cell-adhesion polymer, comprising the steps of: (a) mixing a cell with a cell adhesion polymer to prepare a mixture; And (b) placing the polymer scaffold on the step of the support scaffold and attaching the scaffold to the polymer scaffold; The method may further include a step of culturing cells after attaching the mixed solution to a polymer scaffold.
In addition, the present invention provides a method for preparing a cell culture, comprising the steps of: (a) placing a polymer scaffold on a support of a support support and attaching cells; (b) culturing the cells attached to the polymer scaffold; Wherein the cell culture method further comprises a step of further coating the polymer scaffold with the cell adhesion polymer.
In the cell culture method of the present invention, before adhering cells to the polymer scaffold, the cell culture medium may be adhered to the upper surface of the polymer scaffold placed on the scaffold support, and the scaffold support and the cell culture may be stuck . In addition, when the cells are cultured on the polymer scaffold, a cover for preventing microorganisms from penetrating the top of the cell culture unit may be covered.
In the cell culture method of the present invention, the step of attaching the cells to the polymer scaffold may include applying the cells to the surface of the polymer scaffold or injecting them into the polymer scaffold, Or may be formed by injection or spontaneous infiltration into the interior.
In the cell culture method of the present invention, the step of coating the polymer scaffold or the polymer scaffold with attached cell with the cell adhesive polymer may be carried out by attaching an aqueous solution or a cell adhesive polymer in the form of a hydrogel.
In the cell culture method of the present invention, the step of mixing a cell with a cell-attaching polymer to prepare a mixed solution may include mixing an aqueous solution or a cell adhesive polymer in the form of a hydrogel with a skin cell or a stem cell.
For example, the polymer scaffold may be a biocompatible polymer scaffold, a carcass skin polymer scaffold or a amphipathic polymer scaffold. The cells may be skin cells, stem cells, corneal cells, chondrocytes, hair follicular cells or mucosal cells.
Biocompatible polymer scaffolds, cell adhesion polymers, skin cells and stem cells, which are used as examples, are as follows.
The term " biocompatible polymer " in the present invention refers to a polymeric substance harmless to the human body, which is a synthetic substance which does not release substances harmful to the human body even if it is in direct contact with cells or wounded areas and does not cause adverse effects such as skin irritation, Means a natural polymer material. The biocompatible polymer may be any polymeric material known to be usable as a dressing material, and may be suitably selected by those skilled in the art. Examples of the biocompatible polymer include polyvinyl alcohol (PVA), polyurethane (PU), polyethylene (PE), polyacrylic acid (PAA), polyoxyethylene (POE), polyethylene oxide (PEO), polytetrafluoro (PT), polypropylene (PP), polyethylene terephthalate (PET), polyamide (PA), polyacrylonitrile (PAN), polyester (PES), polyvinyl chloride (PVDF), polysiloxane (PAC), polyacrylic acid (PMA), polysaccharide (PS), polyvinylpyrrolidone (PVA), polysiloxane At least one selected from the group consisting of polyvinylpyrrolidone (PVP), silicone, alginic acid, sodium alginate, cellulose, pectin, chitin, chitosan, gelatin, collagen, fibrin, hyaluronic acid, silk fibroin, natural rubber and synthetic rubber, But is not limited thereto.
The biocompatible polymer scaffold may be obtained by processing the biocompatible polymer into a sheet form and then processing the biocompatible polymer into a sheet form or a nonwoven fabric, a woven fabric, or a cloth prepared from a biocompatible polymer as a raw material, A foam, a hydrocolloid, a hydrogel, and a nonwoven fabric. However, the present invention is not limited thereto and can be used by a person skilled in the art in a suitable form.
In the present invention, the term " skin cells " means cells constituting the skin (epidermis, dermis, subcutaneous fat layer), and the kind thereof is not particularly limited. The skin cells include, for example, keratinocytes, melanocytes, epidermal keratinocytes, melanocytes, fibroblasts that are involved in biosynthesis of collagen and elastin, endothelial cells, It may be a hair follicle stem cell.
In the present invention, the term " stem cell " is not particularly limited. For example, it may be an embryonic stem cell, an induced pluripotent stem cell, or an adult stem cell. The term " embryonic stem cell " includes all embryonic stem cells derived from mammals, such as human embryonic stem cells, and " induced pluripotent stem cells " Means cells that have returned to the cell stage. The term "adult stem cell " is a stem cell derived from adult skin, liver, lung, blood, bone marrow, fat, amniotic membrane, endometrial tissue, cartilage tissue and cord blood.
In the present invention, the term " cell adhesion polymer " refers to a polymer substance having cell adhesion property, which provides adhesion force so that skin cells or stem cells can be fixed to a biocompatible polymer scaffold, The coated dressing material of the present invention provides an adhesive force so that it can adhere to the skin cells of the injured area.
The cell adhesion polymer may be, for example, alginic acid, fibrin, gelatin, collagen or hyaluronic acid, but is not limited thereto, and may be suitably selected by those skilled in the art. The cell adhesion polymer may be used in the form of an aqueous solution or a hydrogel.
As described above, those skilled in the art will understand that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. It is therefore to be understood that the embodiments described above are all illustrative and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention without departing from the scope of the present invention.
10: Cell culture device 20: Cell culture system
30: polymer scaffold 40: cell adhesion polymer
50: cells
100: Support body support part 110:
200: cell culture unit 210: adhesion means
220: fastening means
300: cover
Claims (20)
A cell culture unit positioned above the supporter receiving unit and having upper and lower openings; . ≪ / RTI >
And a step is formed on an upper inner side surface of the supporter receiving part.
Wherein the support support part includes at least one fastening means for fastening the cell culture part to the upper surface.
And a tightening means for tightly adhering a part or whole of these between the supporter receiving portion and the cell culture portion.
And a cover attached to an upper end of the cell culture unit to prevent infiltration of microorganisms.
(a) placing a polymer scaffold on a support of a support and coating the cell scaffold with a cell adhesion polymer; And
(b) attaching the cells to the polymer scaffold coated with the cell adhesive polymer; ≪ / RTI >
Wherein the cell culture method further comprises: (c) culturing the adhered cells.
(a) attaching a polymer scaffold on the support of the support base and attaching the cell; And
(b) coating the cell-bound polymer scaffold with a cell adhesion polymer; ≪ / RTI >
Wherein the cell culture method further comprises the step of (c) culturing the cells on the polymer scaffold after coating with the cell adhesion polymer.
(a) mixing a cell with a cell adhesion polymer to prepare a mixed solution; And
(b) placing the polymer scaffold on the support of the scaffold support and attaching the scaffold to the polymer scaffold; ≪ / RTI >
Wherein the cell culture method further comprises the step of (c) culturing the cells after attaching the mixed solution to the polymer scaffold.
(a) attaching a polymer scaffold on the support of the support base and attaching the cell;
(b) culturing the cells attached to the polymer scaffold; ≪ / RTI >
The cell culture method further comprises (c) coating the polymer scaffold with a cell adhesion polymer.
Wherein the polymer scaffold is a polymer scaffold, carcass skin, or amniotic membrane.
The biocompatible polymer scaffold may be selected from the group consisting of polyvinyl alcohol (PVA), polyurethane (PU), polyethylene (PE), polyacrylic acid (PAA), polyoxyethylene (POE), polyethylene oxide (PEO), polytetrafluoroethylene ), Polypropylene (PP), polyethylene terephthalate (PET), polyamide (PA), polyacrylonitrile (PAN), polyester (PES), polyvinyl chloride (PVC), polyvinylidene fluoride (PGA), polyacrylamide (PAM), polysaccharide (PS), polyvinylpyrrolidone (PVP), polyvinylpyrrolidone (PVP), polyacrylic acid A biocompatible polymer comprising at least one member selected from the group consisting of silicone, alginic acid, sodium alginate, cellulose, pectin, chitin, chitosan, gelatin, collagen, fibrin, hyaluronic acid, silk fibroin, natural rubber, Lt; / RTI >
Wherein the cell is a skin cell, a stem cell, a corneal cell, a cartilage cell, a hair follicle cell, or a mucosal cell.
The skin cells include keratinocytes, melanocytes,
A cell culture method which is an endothelial cell, a hair follicle stem cell, or a fibroblast.
Wherein said stem cells are embryonic stem cells, inducible pluripotent stem cells or adult stem cells.
Wherein the cell adhesion polymer is alginic acid, fibrin, gelatin, collagen or hyaluronic acid.
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| KR1020130031495A KR20140116997A (en) | 2013-03-25 | 2013-03-25 | A cell culture device and cell culture method using the same |
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| KR1020130031495A KR20140116997A (en) | 2013-03-25 | 2013-03-25 | A cell culture device and cell culture method using the same |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109294908A (en) * | 2018-09-07 | 2019-02-01 | 厦门大学 | A kind of bioreactor for simulating intraocular pressure |
| JP2020500522A (en) * | 2016-11-30 | 2020-01-16 | コーニング インコーポレイテッド | Tray to support individual or multiple cell culture wells |
| WO2023132710A1 (en) * | 2022-01-07 | 2023-07-13 | 주식회사 씨티셀즈 | Cell immobilizing apparatus capable of picking cells |
| WO2023140543A1 (en) * | 2022-01-24 | 2023-07-27 | 재단법인대구경북과학기술원 | Upright culture plate device |
-
2013
- 2013-03-25 KR KR1020130031495A patent/KR20140116997A/en active Search and Examination
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2020500522A (en) * | 2016-11-30 | 2020-01-16 | コーニング インコーポレイテッド | Tray to support individual or multiple cell culture wells |
| CN109294908A (en) * | 2018-09-07 | 2019-02-01 | 厦门大学 | A kind of bioreactor for simulating intraocular pressure |
| CN109294908B (en) * | 2018-09-07 | 2021-07-09 | 厦门大学 | Bioreactor for simulating intraocular pressure |
| WO2023132710A1 (en) * | 2022-01-07 | 2023-07-13 | 주식회사 씨티셀즈 | Cell immobilizing apparatus capable of picking cells |
| WO2023140543A1 (en) * | 2022-01-24 | 2023-07-27 | 재단법인대구경북과학기술원 | Upright culture plate device |
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