KR20140085371A - Fusion polypeptide derived from human ferritin - Google Patents

Abstract

The present invention relates to a fusion polypeptide which is derived from a human ferritin and, more specifically, to a fusion polypeptide in which a polypeptide piece displayed in a sequence number 1 and a human-derived ferritin monomer displayed in a sequence number 6 are consecutively connected. The fusion polypeptide of the present invention is able to enclose an effective substance inside by forming a protein cage and can be effectively used in the diagnosis, treatment, and prevention of cancer and arteriosclerosis by improving binding power with a specific molecule of the polypeptide piece, a cell, or a tissue.

Description

Fusion polypeptide derived from human ferritin < RTI ID = 0.0 >

The present invention relates to a fusion polypeptide derived from human ferritin, and more particularly to a fusion polypeptide comprising a polypeptide fragment represented by SEQ ID NO: 1 and a human-derived ferritin monomer represented by SEQ ID NO: 6 in sequence.

IL-4 receptor is known to be overexpressed in various human cancers (colon cancer, lung cancer, uterine cancer and breast cancer), and the development of active polypeptides selectively binding to IL-4 receptor has been applied to diagnosis and treatment of cancer come. In addition, IL-4 acts as a cytokine that increases cell adhesion, and thus acts as a source of atherosclerosis. Therefore, it is believed that an artery that binds to the IL-4 receptor instead of IL-4 and inhibits the binding of IL- Diagnosis and treatment of hardening have been developed.

On the other hand, cage proteins are proteins capable of forming several tens to several hundreds of macromolecules of monomolecular molecular weight by the precise self-assembling properties of low molecular weight monomers. In the natural world, virus capsid protein, ferritin, heat shock protein, and Dps proteins correspond to each other, and each monolith constituting the cage has a very regular and precise interaction with adjacent monomers and the inside of the cage is empty . Since the inside and the outside are separated by the same property as the container of the cage protein as described above, the drug delivery body is frequently used in the medical field.

In veterinary transport of cage proteins, viral vectors and non-viral vectors have been actively studied. To date, adenoviruses have been studied as viral transporters, and ferritin and heat shock proteins have been studied as nonviral transporters. However, in the case of a conventional viral transporter, there has been a problem in the body due to the gene possessed by the virus itself.

Ferritin is a kind of intracellular protein that stores and releases iron. Ferritin is generally in the form of a hollow spherical cage in vivo. The cage is composed of 24 subunits, and the subunit is divided into heavy chain and light chain depending on its structure.

The human-derived ferritin protein has no problem of the stability of the viral carrier, but when binding a targeting moiety to an arbitrary site, there is a problem that the specific binding force is poor or the cage is not formed. Therefore, it is urgent to develop a fusion polypeptide capable of increasing the specific affinity of the target site to be attached and effectively transferring it to the target without any problem in the formation of the cage.

In order to maintain the blood concentration and titer of a protein drug containing a polypeptide as a pharmacological component, a polypeptide is required to be in a stable state because the polypeptide is generally poorly stable and easily degraded and decomposed by proteolytic enzymes in the blood, Drugs need to be administered to patients often. In order to solve such problems, efforts have been made to increase the stability of protein drugs in blood and to maintain the drug concentration in blood for a long time to maximize the drug efficacy. As a method for stabilizing such proteins and inhibiting protease contact and loss of renal function, a method of chemically adding a high-solubility polymer such as poly (ethylene glycol) (PEG) to the surface of a protein drug has been conventionally used . However, when the PEG is bound, the stability of the protein may increase, but the activity of the physiologically active protein is markedly lowered, and as the molecular weight of PEG increases, the reactivity with the protein decreases and the yield decreases.

Accordingly, the present inventors have found that AP-1 peptides are combined with human-derived ferritin to overcome the problems of low binding strength, non-stability, and high immunity of AP-1 peptides discovered for diagnosis, The present inventors have completed the present invention by developing a new technique capable of lowering the immunity while enhancing the cohesion and stability.

The object of the present invention is to provide a fusion polypeptide in which a polypeptide fragment represented by SEQ ID NO: 1 and a human-derived ferritin monomer represented by SEQ ID NO: 6 are sequentially linked.

It is another object of the present invention to provide a fusion polypeptide characterized in that the histidine repeat tag in which 1 to 10 histidine repeats is linked to the polypeptide fragment of SEQ ID NO: 1.

It is another object of the present invention to provide a ferritin protein comprising said fusion polypeptide.

It is another object of the present invention to provide a polynucleotide encoding said fusion polypeptide.

Another object of the present invention is to provide an expression vector comprising the polynucleotide.

Another object of the present invention is to provide a transformant transformed with said expression vector.

It is another object of the present invention to provide a protein cage comprising said fusion polypeptide.

It is another object of the present invention to provide a composition for diagnosing cancer or atherosclerosis comprising the fusion polypeptide as an active ingredient.

Another object of the present invention is to provide a cancer or atherosclerosis diagnostic kit comprising the fusion polypeptide as an active ingredient.

Another object of the present invention is to provide protein nanoparticles comprising the fusion polypeptide.

Another object of the present invention is to provide a protein chip comprising the protein nanoparticles.

Another object of the present invention is to provide a composition for preventing or treating cancer or arteriosclerosis comprising the fusion polypeptide as an active ingredient.

In order to achieve the above object, the present invention provides a fusion polypeptide comprising a polypeptide fragment of SEQ ID NO: 1 and a human-derived ferritin monomer of SEQ ID NO: 6 in sequence.

According to another aspect of the present invention, there is provided a fusion polypeptide, wherein a histidine repeat tag having 1 to 10 histidine repeats is linked to the polypeptide fragment of SEQ ID NO: 1.

According to another aspect of the present invention, there is provided a ferritin protein comprising the fusion polypeptide.

According to another aspect of the present invention, there is provided a polynucleotide encoding the fusion polypeptide.

According to another aspect of the present invention, there is provided an expression vector comprising the polynucleotide.

According to another aspect of the present invention, there is provided a transformant transformed with the expression vector.

According to another aspect of the present invention, there is provided a protein cage comprising the fusion polypeptide.

In order to accomplish another object of the present invention, there is provided a cancer or atherosclerosis diagnostic composition comprising the fusion polypeptide as an active ingredient.

According to another aspect of the present invention, there is provided a cancer or atherosclerosis diagnostic kit comprising the fusion polypeptide as an active ingredient.

In order to accomplish another object of the present invention, there is provided a protein nanoparticle comprising the fusion polypeptide.

According to another aspect of the present invention, there is provided a protein chip comprising the protein nanoparticles.

In order to accomplish still another object of the present invention, there is provided a composition for preventing or treating cancer or atherosclerosis comprising the fusion polypeptide as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention provides a fusion polypeptide in which a polypeptide fragment represented by SEQ ID NO: 1 and a human derived ferritin monomer represented by SEQ ID NO: 6 are sequentially linked.

The fusion peptide of the present invention is characterized in that the polypeptide fragment of SEQ ID NO: 1 and the human-derived ferritin monomer of SEQ ID NO: 6 are sequentially fused.

Ferritin is a type of protein in protein cells that stores and releases iron. Ferritin is generally in the form of a hollow spherical cage in vivo, and the cage is composed of 24 ferritin monomers, and the ferritin monomers are classified into heavy chain and light chain depending on their structure .

The ferritin monomer of the present invention may be any of ferritin monomers regardless of the structure thereof, preferably a ferritin light chain, more preferably a polypeptide consisting of the amino acid sequence of SEQ ID NO: 6. The amino acid sequence shown in SEQ ID NO: 6 is a light chain of human-derived ferritin protein.

In addition, the polypeptide fragment of the present invention can preferably be characterized in that it does not interfere with the fusion between the fusion polypeptide of the present invention or between the fusion polypeptide and the human-derived parity monomer.

The fusion polypeptide of the present invention can be used alone or in combination with a fused polypeptide or other ferritin monomer, such as ferritin, but forms a dimer or a trimmer through a bond between fused polypeptides or a fusion polypeptide with other ferritin monomers such as ferritin Or several monomers may form a cage protein to exhibit a new function or to further increase the binding specificity with other substances. Therefore, the polypeptide fragment of the present invention may contain the fusion polypeptide or the fusion polypeptide and the human-derived paratin monomer Lt; / RTI >

Further, the fusion polypeptide of the present invention is characterized by being specifically bound to a target substance. The target substance refers to a substance exhibiting binding affinity with a polypeptide fragment fused to the fusion polypeptide of the present invention.

The binding affinity refers to the strength of a property of binding between biomolecules.

In addition, any of the above-mentioned desired substances can be used and include both in vivo substances and in vitro substances. In vivo substances include all organs, tissues, cells, receptors on the cell surface, intracellular proteins including enzymes, intercellular substances, nucleic acids, toxic substances from outside, therapeutic substances, and test substances. The in vitro material includes all kinds of organic and inorganic compounds (various resins, slide glasses, plates, films) or various test objects for various tests. Preferably, the target substance is an IL-4 receptor.

In the present invention, the fusion polypeptide of the present invention provides a fusion polyp peptide characterized in that a histidine repeat tag in which 1 to 10 histidine repeats is linked to the polypeptide fragment of SEQ ID NO: 1.

In the present invention, the histidine tag may be a tag in which 1 to 10 histidine repeats, and preferably a histidine tag in which histidine is repeated 6 times.

The present invention also provides a ferritin protein comprising the fusion polypeptide of the present invention.

The ferritin protein of the present invention may be a complex protein in which the fusion polypeptide of the present invention is regularly arranged as a unit, and more preferably, 24 fusion polypeptides of the present invention may be three-dimensionally and regularly arranged. In addition, the ferritin protein of the present invention is produced by binding ferritin protein monomers, and generally has a spherical cage shape in vivo.

In one embodiment of the present invention, Escherichia coli was transformed using an expression vector containing the fusion polypeptide of the present invention (hereinafter referred to as His_AP1_ferritin) to mass-produce the ferritin protein. As a control, the fusion protein of the present invention was not included, and a protein was produced by the same procedure as above with an expression vector containing only a histidine tag (hereinafter referred to as His_ferritin). As a result, it was confirmed that His_AP1_ferritin protein and His_ferritin protein were purified to about 23 kDa on SDS-PAGE (FIG. 2).

The present invention also provides polynucleotides encoding the fusion polypeptides of the present invention.

The polynucleotide of the present invention may preferably be a polynucleotide represented by SEQ ID NO: 7.

The present invention also provides an expression vector comprising the polynucleotide of the present invention.

The expression vector of the present invention is characterized in that it comprises the polynucleotide of the present invention. As an expression vector capable of containing the polynucleotide of the present invention, plasmids derived from Escherichia coli (pBR322, pBR325, pUC118, pUC119, pET30a, pET30c, pGEX-GST), plasmids derived from Bacillus subtilis (pUB110 and pTP5), yeast-derived plasmids (YEp13, YEp24 and YCp50), and Ti plasmids; animal viruses such as retrovirus, adenovirus or vaccinia virus; Insect viruses such as viruses and plant viruses can be used, and binary vectors such as pPZP, pGA and pCAMBIA series can be used. Those skilled in the art can select a vector suitable for introducing the polynucleotide sequence of the present invention. In the present invention, any vector can be used as long as it can introduce the polynucleotide sequence of the present invention into a host cell. Preferably, however, vectors designed to facilitate induction of protein expression and separation of expressed proteins can be used. More preferably, the pET29a (+) vector, which is a recombinant vector containing the polynucleotide of the present invention, can be used.

In one embodiment of the present invention, pET29a (+) was treated with restriction enzymes NdeI and XhoI at 37 ° C for 1 hour, purified, and bound to His_AP1_ferritin and His_ferritin using T4 DNA ligase. As a result, His_AP1_ferritin expression vector and His_ferritin expression vector were obtained (FIG. 1).

The present invention also provides a transformant transformed with an expression vector of the present invention.

The transformant of the present invention is characterized by being transformed with the expression vector of the present invention. Transformation with the above expression vector can be carried out by a transformation technique known to a person skilled in the art. Preferably, microprojectile bombardment, electroporation gene transfer, calcium phosphate (CaPO ₄ ) precipitation, calcium chloride (CaCl ₂ ) precipitation, PEG-mediated fusion, microinjection microinjection and liposome-mediated method. The transformant may be Escherichia coli, Bacillus subtilis, Streptomyces, Pseudomonas, Proteus, But are not limited to, Proteus mirabilis, Staphylococcus, Agrobacterium tumefaciens, and the like.

The present invention also provides a protein cage comprising the fusion polypeptide of the present invention.

Protein cage is a protein cage formed by the precise self-assembling nature of low molecular weight monomers and having an internal space. Virus capsid protein, ferritin, heat shock protein, and Dps protein. The protein cage of the present invention is characterized in that the fusion polypeptide of the present invention is contained as a monomer (monomer) constituting the protein cage. The protein cages of the present invention may be composed of the fusion polypeptides of the present invention alone or in combination with the fusion polypeptides of the present invention and other ferritin protein monomers.

In one embodiment of the present invention, a protein prepared by performing Fast Protein Liquid Chromatography (FPLC) on two proteins, His_AP1_ferritin and His_ferritin, produced in another embodiment of the present invention and wild type ferritin, Was well formed. As a result, in the drawn standard curve graph, the size of His_AP1_ferritin and His_ferritin curve were similar to those of wild-type ferritin, confirming that 24-mer formation was well performed (FIG. 3).

In another embodiment of the present invention, it was confirmed by using a biotransmission microscope that proteins of His_AP1_ferritin and His_ferritind were well formed in the cage. As a result, it was confirmed that the above two proteins formed well in the 24-mer cage (FIG. 4).

The present invention also provides a composition for diagnosing cancer or arteriosclerosis comprising the fusion polypeptide of the present invention as an active ingredient and a diagnostic kit.

Diagnosis of the present invention means identifying the presence or characteristic of a pathological condition. For purposes of the present invention, the diagnosis is to identify the presence or characteristic of cancer or arteriosclerosis. The fusion polypeptide contained in the diagnostic composition of the present invention may have the amino acid sequence shown in SEQ ID NO: 1 and SEQ ID NO: 6,

The polypeptide fragment of SEQ ID NO: 1 of the present invention has a characteristic of specifically binding to an IL-4 receptor.

IL-4 (interleukin-4) is a cytokine with a variety of immunoregulatory functions secreted by T-helper 2 (Th2) lymphocytes, eosinophils, and mast cells. IL-4 induces the differentiation of naive T-helper (naive Th) into Th2 lymphocytes and induces the production of cytokines such as IL4, IL5, IL9 and IL13. It also induces the secretion of IgE (immunoglobin E) by B lymphocytes. In particular, IL-4 is known to play an important role in airway obstruction and inflammation by inducing mucin gene expression and mucus secretion in asthma (Paul, Blood, 1991; 77: 1859-1870). Thus, IL4 is a key substance in the allergic inflammatory response, and thus can be effectively applied to the treatment of allergic diseases if it can adequately inhibit the effect regulated by IL4.

In addition, IL-4 is found at higher concentrations than normal tissues in many cancer tissues, and a large amount of IL-4 is produced in tumor-infiltrating lymphocytes (TILs) (Shurin, Springer Semin Immunopathol, 1999 21: 339). IL-4 acts on chronic lymphocytic leukemia B cells and causes resistance to apoptosis of these cells (Dancescu, J Exp Med, 1992; 176: 1319). In addition, IL-4 is also synthesized in tumor cells and cancer stem cells, and it has recently been reported that cancer cells are resistant to apoptosis through IL-4 receptor on the surface of cancer cells (Todaro, Cell Death Differ, 2008; 15: Todaro, Cell Stem Cell, 2007, 1: 389-402). IL-4 receptor is expressed much more in normal cells than in normal cells in various cancer cells such as non-small cell lung cancer, brain tumor, breast cancer, bladder cancer, pancreatic cancer, kidney cancer, prostate cancer, kidney cancer and caposi's sarcoma. The IL-4 receptor is a promising target for male tumors, given the ability of cancer cells to acquire resistance to IL-4 receptor anticancer drugs and the high expression level in cancer cells. Currently, IL-4 itself has been partially modified, and then it has been reported that a fusion protein with psuedomonas toxin is used to kill cancer cells by targeting the cancer cells and injecting toxins into cells (Joshi, Cancer Res, 2001 ; Kawakami, Clin Cancer Res, 2002; 8: 3503-3511); Kawakami et al., Cancer Res. 2005; 65: 8388-8396;

In addition, IL-4 is found in atherosclerotic tissue at a higher concentration than normal tissue, and induces VCAM-1 and MCP-1 expression in vascular endothelial cells, resulting in migration of mononuclear cells, T lymphocytes, basophils and eosinophils (Sasaguri et al., Atherosclerosis, 1998; 138: 247-253; Lee et al., J Mol Cell Cardiol, 2001; 33: 83-94). More importantly, the genetic deficiency of IL-4 in atherosclerotic model mice with genetically deficient LDL receptors or ApoE proteins has been reported to reduce the size of atherosclerotic lesions of the aorta (Davenport et al., Am J Pathol, 2003; 163: 1117-1125; King et al., Arterioscler Thromb Vasc Biol, 2002; 22: 456-461). Thus, IL-4 plays a role in the development of atherosclerosis, and if it can antagonize the action of IL-4, it will be effective in the diagnosis, treatment or prevention of arteriosclerosis.

In one embodiment of the present invention, the His_AP1_ferritin protein and the His_ferritin protein were bound to A549, Hela and 4T1 cells expressing the IL-4 receptor, and analyzed by fluorescence activated cell sorting (FACS) In order to investigate the degree of binding to IL-4 receptor, IL-4 receptor blockade was performed on A549 cells and HeLa cells, and His_AP1_ferritin protein was bound to IL-4 receptor receptor (Fig. 5).

In another embodiment of the present invention, by directly observing the binding of His_AP1_ferritin protein and His_ferritin protein to Hela and 4T1 cells using confocal microscopy, it was confirmed that His_AP1_ferritin protein binds better than His_ferritin protein (FIG. 6).

In another embodiment of the present invention, it was confirmed that cell uptake occurred using z-stack. As a result, it was confirmed that the His_AP1_ferritin protein not only increased binding to Hela and 4T1 cells but also uptake in cytoplasm 7).

Therefore, the fusion polypeptide of the present invention exhibits a high binding affinity to the IL-4 receptor and specifically binds to the IL-4 receptor. Animal experiments were carried out to confirm that these fusion polypeptides maintained stability in animals, and stability in animal experiments was ensured through changes in animal weight and tumor size.

In addition, in an example to confirm tumor targeting in animals with tumors, the fusion markers of the fusion polypeptides were superior in tumor targeting, and this tumor targeting was confirmed by the IL-4 receptor of the tumor cells. In addition, we confirmed that the marking ability was confirmed in real time and continued for 24 hours.

Based on these results, the protein cage comprising the fusion polypeptide of the present invention effectively delivers the substance in the cage into the cell, thus providing a composition for diagnosing cancer and arteriosclerosis using the fusion polypeptide of the present invention and a diagnostic kit.

The composition and diagnostic kit for cancer or atherosclerosis of the present invention have an effect of effectively diagnosing, preventing and treating cancer or arteriosclerosis which is an IL-4 mediated inflammatory disease.

The disease to which the composition of the present invention may be applied may be cancer. The type of the cancer is not particularly limited and examples thereof include breast cancer, colon cancer, lung cancer, small cell lung cancer, gastric cancer, liver cancer, blood cancer, bone cancer, pancreatic cancer, skin cancer, head or neck cancer, skin or intraocular melanoma, Ovarian cancer, ovarian cancer, rectal cancer, proximal cancer, colon cancer, breast cancer, fallopian tube carcinoma, endometrial carcinoma, cervical cancer, vaginal cancer, vulvar carcinoma, Hodgkin's disease, esophageal cancer, small bowel cancer, endocrine cancer, thyroid cancer, Renal cell carcinoma, renal pelvic carcinoma, renal pelvic carcinoma, CNS tumor, primary CNS lymphoma, spinal cord tumor, brainstem glioma, and pituitary adenoma Lt; / RTI > Preferably lung cancer, uterine cancer or breast cancer.

The present invention also provides protein nanoparticles comprising the fusion polypeptide and protein chips comprising the protein nanoparticles.

The protein nanoparticle means a spherical protein particle having a diameter of nanometer size.

The protein nanoparticles of the present invention are characterized by comprising the fusion polypeptide of the present invention.

The protein chip of the present invention is characterized by including the protein nanoparticles of the present invention.

The protein nanoparticles of the present invention are effective for diagnosis, prevention and treatment of cancer or atherosclerosis, which is an IL-4 receptor mediated disease, by including the fusion polypeptide of the present invention having a high binding affinity to the IL-4 receptor.

The protein chip refers to a biochip that integrates tens or thousands of different peptides or proteins capable of reacting with a specific biomolecule on a solid surface and combines with various biomolecules for effective analysis. In the production of the protein chip of the present invention, it is preferable to use a polymer or gel such as glass, modified silicone, tetrafluoroethene, polystyrene or polypropylene as a substrate, and the protein nanoparticles of the present invention Protein chips can be used as biomarkers or screening systems for new drug candidates. Further, the protein chip of the present invention includes protein nanoparticles containing the fusion polypeptide of the present invention, which is effective for diagnosis, prevention and treatment of cancer or arteriosclerosis, which is an IL-4 receptor mediated disease, and is excellent for diagnosing cancer or arteriosclerosis Effect.

The present invention also provides a composition for preventing or treating cancer or atherosclerosis comprising the fusion polypeptide of the present invention as an active ingredient.

The pharmaceutical composition according to the present invention may contain the fusion polypeptide of the present invention alone or may further comprise one or more pharmaceutically acceptable carriers. The term " effective amount " as used herein refers to an amount exhibiting a further reaction than the negative control, preferably an amount sufficient to treat cancer or arteriosclerosis.

The 'subject' may be an animal, preferably a mammal, especially an animal, including a human, and may be an animal derived cell, tissue, organ, or the like. The subject may be a patient requiring treatment.

The term " pharmaceutically acceptable " as used herein means a non-toxic composition which is physiologically acceptable and which, when administered to humans, does not inhibit the action of the active ingredient and does not normally cause an allergic reaction such as gastrointestinal disorder, dizziness, .

The type of the cancer is not particularly limited and examples thereof include breast cancer, colon cancer, lung cancer, small cell lung cancer, gastric cancer, liver cancer, blood cancer, bone cancer, pancreatic cancer, skin cancer, head or neck cancer, skin or intraocular melanoma, Ovarian cancer, ovarian cancer, rectal cancer, proximal cancer, colon cancer, breast cancer, fallopian tube carcinoma, endometrial carcinoma, cervical cancer, vaginal cancer, vulvar carcinoma, Hodgkin's disease, esophageal cancer, small bowel cancer, endocrine cancer, thyroid cancer, Renal cell carcinoma, renal pelvic carcinoma, renal pelvic carcinoma, CNS tumor, primary CNS lymphoma, spinal cord tumor, brainstem glioma, and pituitary adenoma Lt; / RTI > Preferably lung cancer, uterine cancer or breast cancer.

In the pharmaceutical composition according to the present invention, the fusion polypeptide may be administered in various forms of oral and parenteral administration at the time of clinical administration. In the case of formulation, the commonly used fillers, extenders, binders, wetting agents, Or a diluent or an excipient such as a surfactant.

Solid formulations for oral administration include tablets, patients, powders, granules, capsules, troches, and the like, which may contain one or more excipients such as starch, calcium carbonate, sucrose, sucrose, lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like may also be used. Liquid preparations for oral administration include suspensions, solutions, emulsions or syrups. In addition to water and liquid paraffin which are commonly used simple diluents, various excipients such as wetting agents, sweetening agents, fragrances, preservatives and the like are included .

Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories.

The therapeutic compositions of the present invention may be formulated with any physiologically acceptable carrier, excipient or stabilizer (Remington: The Science and Practice of Pharmacy, l9th Edition, Alfonso, R., ed., Mack Publishing Co. )) And an antibody having a desired purity can be prepared in the form of a lyophilized cake or an aqueous solution for storage. Acceptable carriers, excipients or stabilizers are nontoxic to the recipient at the dosages and concentrations employed and include buffer solutions such as phosphoric acid, citric acid and other organic acids; Antioxidants including ascorbic acid; Low molecular weight (less than about 10 residues) polypeptides; Proteins, such as serum albumin, gelatin or immunoglobulins; Hydrophilic polymers such as polyvinylpyrrolidone; Amino acids such as glycine, glutamine, asparagine, arginine or lysine; Monosaccharides, disaccharides, and other carbohydrates including glucose, mannose or dextrin; Chelating agents such as EDTA; Sugar alcohols such as mannitol or sorbitol; Salt-forming counterions such as sodium; And / or non-ionic surfactants such as tween, pluronics or polyethylene glycol (PEG).

In addition, administration of the fusion polypeptide of the present invention to the human body can be administered to a patient in a single dose, and can be administered by a fractionated treatment protocol administered for a long time in multiple doses . In the pharmaceutical composition of the present invention, the content of the active ingredient may be varied depending on the degree of the disease. Preferably, the dosage of the fusion polypeptide of the invention may be from about 0.0001 mg to 100 mg, most preferably from 0.001 mg to 10 mg per kilogram of patient body weight per day. The dosage is determined in consideration of various factors such as age, weight, health condition, sex, severity of disease, diet and excretion rate, and therefore, considering this fact, , It will be possible to determine an appropriate effective dose depending on the particular use of the composition of the present invention. The pharmaceutical composition according to the present invention is not particularly limited to its formulation, administration route and administration method as long as the effect of the present invention is exhibited.

The composition of the present invention can be administered to mammals including humans by any method. For example, it can be administered orally or parenterally. Parenteral administration methods include, but are not limited to, intravenous, intramuscular, intraarterial, intramedullary, intrathecal, intracardiac, transdermal, subcutaneous, intraperitoneal, intranasal, enteral, topical, sublingual or rectal administration Lt; / RTI >

The pharmaceutical composition of the present invention can be formulated into oral preparations or parenteral administration preparations according to the administration route as described above.

In the case of a preparation for oral administration, the composition of the present invention may be formulated into a powder, a granule, a tablet, a pill, a sugar, a tablet, a liquid, a gel, a syrup, a slurry, . In the case of a preparation for parenteral administration, it can be formulated by a method known in the art in the form of injection, cream, lotion, external ointment, oil, moisturizer, gel, aerosol and nasal aspirate. These formulations are described in Remington's Pharmaceutical Science, 15th edition, 1975. Mack Publishing Company, Easton, Pennsylvania 18042, Chapter 87: Blaug, Seymour, a commonly known formulary for all pharmaceutical chemistries.

The pharmaceutical composition of the present invention can be used alone or in combination with methods for the prevention or treatment of cancer or arteriosclerosis or using surgery, hormone therapy, chemotherapy and biological response modifiers.

As described above, the present invention provides a fusion polypeptide comprising the polypeptide fragment of SEQ ID NO: 1 and the human-derived ferritin monomer of SEQ ID NO: 6 in sequence.

The fusion polypeptide of the present invention is capable of encapsulating an effective substance in the inside of a protein cage, and is highly effective in diagnosing, treating, and preventing cancer and arteriosclerosis by greatly enhancing the binding force of a polypeptide fragment with a specific molecule, cell, or tissue .

Figure 1 shows the structure of His_ferritin expression vector (A) and His_AP1_ferritin expression vector (B). His_ferritin is an expression vector comprising a histidine tag and human-derived ferritin of SEQ ID NO: 6, and His_AP1_ferritin comprises a fusion polypeptide of the present invention in which a histidine tag, a polypeptide fragment of SEQ ID NO: 1 and human-derived ferritin of SEQ ID NO: 6 are sequentially linked Lt; / RTI >
Figure 2 shows the result of SDS-PAGE analysis of His_ferritin protein and His_AP1_ferritin protein.
Figure 3 compares the protein sizes of His_ferritin and His_AP1_ferritin with wild type ferritin and fast protein liquid chromatography (Abs @ 280nm: Absorbance at 280nm, Flow: Flow (ml), Ferritin (dashed line): wild type ferritin -type ferritin), His_ferritin (thin green line): ferritin with histidine bound, His_AP1_ferritin (bold red line): ferritin with histidine and AP1
FIG. 4 shows the formation of a 24-mer cage of His_ferritin protein and His_AP1_ferritin protein by bio-transmission electron microscope (His_ferritin: Histidine-bound ferritin, His_AP1_ferritin: Histidine and AP1-conjugated ferritin).
FIG. 5 is a graph comparing the degree of cell binding using fluorescent substance-expressing cell selection (A549: lung cancer cell line, HeLa: cervical cancer cell line, 4T1: breast cancer cell line, control: untreated group, His_ferritin: ferritin conjugated with histidine, His_AP1_ferritin: Histidine and AP1-conjugated ferritin treated group, His_AP1_ferritin // IL4R blocking: Pretreatment of IL4 Receptor blocking Ab, followed by treatment with ferritin combined with histidine and AP1).
FIG. 6 shows the degree of cell binding in confocal microscopy (A549: lung cancer cell line, HeLa: cervical cancer cell line, His_ferritin: ferritin-treated group with histidine, His_AP1_ferriti: ferritin treated with histidine and AP1).
FIG. 7 shows the cell uptake through z-stack with confocal microscopy (A549: lung cancer cell line, HeLa: cervical cancer cell line, His_ferritin: ferritin treated group with histidine, His_AP1_ferritin: ferritin treated with histidine and AP1) .
Figure 8 shows the binding affinity determined by surface plasmon resonance analysis. (A, B: The resonance value proportional to the amount of His-AP1_ferritin, which is expressed by concentration, is passed to the IL-4 receptor immobilized on the surface of the analyzer.) C: The saturation resonance value was plotted according to each concentration and then the binding affinity (Kd) was calculated by KaleidaGraph program. The control group was His-ferritin.
FIG. 9 shows changes in the weight of the mice and the size of the tumors ((a): change in body weight of a mouse, (b): tumor growth pattern) in order to confirm the safety of the polypeptide in an animal model.
FIG. 10 is a photograph of the result of a confocal microscopic observation to confirm the tumor marker of the polypeptide ((a): tumor tissue in the case of injecting His_ferritin; (b) tumor tissue in the case of injecting His_AP1_ferritin).
11 is a photograph of a result of observation of small animal imaging to confirm the tumor targeting property of the polypeptide.

Hereinafter, the present invention will be described in detail.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

≪ Example 1 >

Production and purification of expression vectors

<1-1> Preparation of Expression Vector

A recombinant method was used to produce a fusion protein of AP1 peptide linked to human ferritin light chain.

Specifically, His_AP1_ferritin was inserted into the His tag and AP1 amino acid sequence (SEQ ID NO: 1) inserted in front of SSQIR in the direction of N term, and His_ferritin inserted only His Tag into the control. The expression vector was pET29a (+), an E. coli expression vector.

To synthesize the nucleotide sequence of AP1 peptide, oligomers were ordered as shown in Table 1 below, and the mixture was mixed at a molar ratio of 1: 1 and then diluted to a final concentration of 1 pmol / μl. The pET29a (+) expression vector was then treated with NdeI and XhoI restriction enzyme (purchased from TAKARA) for 1 hour at 37 ° C, incubated at 95 ° C for 5 minutes in a heat block, After purification, the AP1 gene was ligated using T4 DNA ligase (FIG. 1).

primer Primer sequence SEQ ID NO: S1_AP1_
ferritin Forward primer gatccatatgcatcatcaccaccatcacggcagccgaaaacggctcgacaggaacggcagcatgagctcccagattcgtca 2 Reverse primer gatcctcgagttagtcgtgcttgagagtgagcc 3 WT_
ferritin Forward primer gatccatatgcatcatcaccaccatcacggcagcatgagctcccagattcgtca 4 Reverse primer gatcctcgagttagtcgtgcttgagagtgagcc 5

&Lt; 1-2 > Expression and purification of vectors Purification

Escherichia coli was transformed with the prepared expression vector to produce a fusion protein in a large amount. Specifically, the His_AP1_ferritin expression vector, in which the His tag and the His tag and the AP1 peptide were inserted, was transformed into the BL21 host cell and incubated at 37 ° C in LB medium. When OD ₆₀₀ value reached 0.5, IPTG 0.5mM To induce expression. The cells were further cultured at 18 ° C for 18 hours, and the cells were harvested and disrupted using lysis buffer (50 mM Tris, 100 mM NaCl, 1 mM EDTA, 1% tripton X-100, 1 mM PMSF, 1 mM DTT) (4 degrees, 13000 rpm, 30 minutes) was performed. His_ferritin was used for purification of the affinity chromatography only after centrifugation, and His_AP1_ferritin was used for affinity chromatography purification of the precipitated pellet.

In the case of His_ferritin, the supernatant was bound to the Ni-NTA beads and the protein loaded onto the Ni-NTA column. The wash buffer (20mM Tris, 500mM NaCl, 20mM imidazole) was poured and elution buffer (50mM Tris, 100mM NaCl, 200mM Histidine) was used to elute the proteins attached to the beads, followed by SDS electrophoresis (15% acrylamide gel).

In the case of His_AP1_ferritin, the pellet after centrifugation was dissolved in a binding buffer (20 mM Tris, 500 mM NaCl, 5 mM imidazole) containing 8 M urea, followed by washing and elution buffer supplemented with 8 M urea. Afterwards, dialysis was performed to remove 8M urea and protein electrophoresis was performed on 15% acrylamide gel.

As a result, both proteins were purified to a size of about 23 kDa by SDS-PAGE (FIG. 2).

< Example  2>

Cage formation test of AP1 peptide insertion ferritin

<2-1> Ferritin protein formation test using Fast Protein Liquid Chromatography (FPLC)

Gel Permeation Liquid Chromatography (GPC) was performed to determine whether His_ferritin and His_AP1_ferritin proteins formed well on 24 mer.

GPC was performed on a Superdex 200 10/300 GL column (GE Healthcare), and the injection volume was 50 μl. The buffer was 50 mM Tris-HCl (8.0), 100 mM NaCl, and 2000 mM Histidine. A standard curve was constructed using wild-type ferritin (molecular weight 440KD), BSA (molecular weight 67KD), ovalbumin 45KD, Chymotrypsin A 25KD and Ribonuclease A 13KD. His_ferritin and His_AP1_ferritin were compared with the standard curve, Respectively.

As a result, the size of wild-type ferritin, His_ferritin and His_AP1_ferritin were similar, and 24-mer was thought to be well-formed (Fig. 3).

<2-2> Biological transmission electron microscope

To confirm whether His_ferritin and His_AP1_ferritin proteins form well in the cage, biotransmission electron microscopy was taken. The concentration of each sample was 0.1 mg / ml, and the photograph was taken by KBSI. The sample was negatively stained with uranyl acetate and was run on the tecnai machine of the FEI manufacturer.

As a result, both His_ferritin and His_AP1_ferritin were found to form well in the 24-mer cage, and it was further confirmed that they formed a cage at a similar ratio at the same concentration (FIG. 4).

< Example  3>

Cell binding test

<3-1> FACS Cell binding test using

A549 cell (human lung cancer), Hela cell (human cervical cancer), and 4T1 cell (mouse mammary cancer), which express IL-4 receptor, were tested for the binding of AP1 peptide- Was used. First, raise the cell to a 100-pi plate until the confulence reaches 80%. The cells were washed twice with PBS, and the cells were removed with trypsin / EDTA. 2x10 ⁵ / 100ul cells were incubated in PBS containing 1% bovine serum albumin (BSA) for 30 min. Respectively. Blocking experiments were further performed on A549 cells. Human IL-4Rα antibody (MAB230) was diluted 1: 1000 in PBS containing 1% BSA and incubated at 4 ° C for 1 hour to block the IL-4 receptor. After washing twice with PBS, His_ferritin and His_AP1_ferritin were diluted in 50 mM Tris, 100 mM NaCl and 2000 mM Histidine buffer at a concentration of 10 uM and then allowed to bind (4 ° C, 20 min). After washing twice with PBS, ferritin light chain antibody (D-18, sc-14420) was diluted 1: 400 in PBS buffer containing 1% BSA and incubated at 4 ° C for 30 minutes. After washing twice with PBS, Alexa Fluor 488 donkey anti-goat IgG (H + L) antibody was diluted 1: 200 in PBS buffer containing 1% BSA and incubated at 4 ° C for 30 minutes. After washing twice with PBS, cells were suspended in 500 μl PBS and FACS was performed.

As a result, it was confirmed that the binding of cell and His_AP1_ferritin is dependent on the IL4 receptor by confirming that the degree of binding is decreased when the IL4 receptor blocking Ab is preincubated in A549 cell and HeLa cell (FIG. 5 ).

<3-2> Confocal  microscope( confocal microscope ) Cell binding test

This experiment was performed to confirm that His_AP1_ferritin binds well to the IL-4 receptor expressed in Hela cells through confocal microscopy.

1x10 ⁴ / 200ul seeded in an 8 well slide chamber and incubated overnight to adhere well to the chamber. After washing twice with PBS, His_ferritin and His_AP1_ferritin were diluted in 20 mM Tris, 150 mM NaCl, and 180 mM Histidine buffer at a concentration of 10 uM and incubated at 37 ° C for 1 hour. After washing twice with PBS, add cold stored methanol and incubate at 20 ° C for 20 minutes to permeabilize the cell membrane. After washing twice with PBS, PBS containing 1% BSA was added and blocked at room temperature for 30 minutes. After washing twice with PBS, anti-His-probe (H-15) Alexa Fluor 647 antibody was diluted 1:40 in PBS and incubated overnight at 4 ° C. After washing twice with PBS, DAPI was incubated in the dark for 3 minutes, stained with cells, mounted with antifade reagent, covered with cover glass and confocal microscopy. 4T1 cells were also tested in the same manner.

As a result, it was confirmed that His_AP1_ferritin had a green signal with a cell surface as its center, and it was found to bind better than His_ferritin (FIG. 6).

< Example  4>

Receptor-mediated endocytosis receptor mediated endocytosis )

This experiment was performed to determine whether His_AP1_ferritin uptakes into the cell through the IL-4 receptor expressed in Hela cells.

First seed 1x10 ⁴ / 200ul in an 8 well slide chamber and incubate overnight to adhere well to the chamber. After washing twice with PBS, His_ferritin and His_AP1_ferritin were diluted in 20 mM Tris, 150 mM NaCl, and 180 mM Histidine buffer at a concentration of 10 uM and incubated at 37 ° C for 1 hour. After washing twice with PBS, add cold stored methanol and incubate at 20 ° C for 20 minutes to permeabilize the cell membrane. After washing twice with PBS, PBS containing 1% BSA was added and blocked at room temperature for 30 minutes. After washing twice with PBS, anti-His-probe (H-15) Alexa Fluor 647 antibody was diluted 1:40 in PBS and incubated overnight at 4 ° C. After washing twice with PBS, DAPI was incubated in the dark for 3 minutes, stained with cells, mounted with antifade reagent, covered with cover glass and confocal microscopy. 4T1 cells were also tested in the same manner. We also observed confocal microscopy through Z-stack to confirm uptake in cytoplasm. Since the cell is attached to the bottom of the plate but has a three-dimensional shape, it is difficult to distinguish whether ferritin is attached to the cell surface or uptake if it is not observed through the Z-stack. From high to high.

As a result, in the case of His_AP1_ferritin, cell surface binding was increased in both Hela cell and 4T1 cell, but Z-stack was also observed, and it was confirmed that uptake in cytoplasm also occurred well (FIG.

< Example  5>

Binding force analysis of active polypeptide

<5-1> Surface Plasmon  resonance( SPR : Surface plasmon resonance ) analysis

In the SPR experiment, the reaction of the sample was tested on two channels simultaneously. The left channel was coated with the protein (IL-4 receptor) to be reacted and the right channel was coated or not coated with the control. And thus non-specific reactivity can be excluded. In this experiment, the empty channel was used as a control channel, blocking the surface activated by ethanolamine without coating other proteins. In the left channel of the carboxymethyl dextran chip, 50 μl of a 0.7 μg / ml IL-4 receptor protein was added to 50 μl of a 200 mM NaOAC pH 4.0 solution, and the mixture was applied at a rate of 25 μl / min for 7 minutes. The surface was blocked with 1M ethanolamine pH 8.5 solution at a rate of 25ul / min for 7 minutes to reduce nonspecific adsorption. All the experiments were carried out at a temperature of 25 ° C and the flow rate was 25ul / min. The buffer was subjected to filtering and degassing in 20 mM Tris-HCl pH 8.0, 150 mM NaCl and 180 mM Histidine, and the concentrations of His ferritin and His_AP1_ferritin were measured at concentrations of 0.651 nM, 2.604 nM, 5.208 nM, 10.416 nM, 20.833 nM and 41.666 nM, respectively Min for 20 min, and 2M NaCl was added to each well to wash the bound protein. His_AP1_ferritin specifically binds to the IL-4 receptor, resulting in a change in surface plasmon resonance, which is converted to a resonance unit (RU) to obtain a sensorgram for each concentration. The right channel values were subtracted from the left channel values at each concentration and the specific binding values were plotted on the graph. The saturation RU and protein concentration were plotted in KaleidaGraph and the dynamic binding constant (Kd) was calculated by applying simple bimolecular model. Averages and deviations of the experiments were shown three times.

As a result, it was confirmed that His_ferritin did not bind to the IL4 receptor and His_AP1_ferritin had a strong binding affinity of (3.68 ± 1.3) × 10 ^-9 M (FIG. 8).

< Example  6>

Stability Analysis in Animal Tumor Model

<6-1> Analysis of mouse body weight and tumor size change

HeLa cells (1 × 10 ⁷ / PBS 150 μl) are injected subcutaneously into the flank site of the balb / c nude mouse and the tumor is allowed to grow to a volume of about 100 mm ³ for 4 weeks. Mice were divided into two groups, one group receiving no treatment, and the other group intravenously injecting His_AP1_ferritin (2 mg / kg) through the tail vein every other day. In order to confirm the stability of the intravenously injected polypeptide, weight change and tumor volume change of the mice were observed for 15 days, and the results between the two groups were compared and analyzed (FIG. 9).

As a result, as shown in Fig. 9, it was confirmed that there was no difference in body weight and tumor growth between the two groups.

< Example  7>

Tumor mark aptitude analysis in animal tumor models

<7-1> Identification of Polypeptide Tumor Marking

HeLa cells (1 × 10 ⁷ / PBS 150 μl) are injected subcutaneously into the flank site of the balb / c nude mouse and the tumor is allowed to grow to a volume of 200 mm ³ for 5 weeks. Mice were divided into two groups, His_ferritin polypeptides in one group and His_AP1_ferritin polypeptides (2 mg / kg) in the other group were intravenously injected via the tail vein. Two hours after intravenous injection, the mice were anesthetized and the tumor was removed, and the mice were sacrificed. The removed tumor was washed in PBS buffer and cryo-block was made using OCT compound after 12 hours in 4% paraformaldehyde. Immunohistochemical staining was performed with a cryotome instrument on a frozen section with a thickness of 8 μm. Ab-FITC (ab-FITC, ab77825, abcam) against His-Tag (HHHHHH) was used to bind to the polypeptide. FITC bound to Ab was observed through confocal microscopy.

As shown in FIG. 10 (a), His-ferritin could not be observed in the tumor tissues in the group injected with His_ferritin, but His-AP1_ferritin was found in the tumor tissues in the group injected with His_AP1_ferritin FITC signal. Thus, it was confirmed that the fusion polypeptide of the present invention has tumor targeting ability.

<7-2> IL Tumor marking by receptor-4

Additional experiments were conducted to determine whether the tumor targeting of the His_AP1_ferritin polypeptide is due to the IL-4 receptor in tumor cells. Immunohistochemical staining of HeLa cell tumor tissues was performed by frozen sections. His_AP1_ferritin polypeptide was detected by His-Tag Ab (mouse anti-His tag Ab-FITC, ab77825, abcam) and the IL-4 receptor of the tumor cell was detected by IL-4R Ab (rabbit polyclonal Ab to IL4R, ab61099, -rabbit Ig-G TR, sc-2780, santacruz). Immunostained slides were analyzed by confocal microscopy and compared.

As a result, as shown in FIG. 10 (b), the His_AP1_ferritin polypeptide (green signal) present in the tumor tissue was found to match the position of the IL-4 receptor (red signal) of the tumor cell. These results indicate that His_AP1_ferritin polypeptide binds to the IL-4 receptor even in tumor cell experiments expressing the IL-4 receptor.

<7-3> Tumor marking up to 24 hours in real time

Observations using the IVIS spectrum, a small animal imaging device, were planned to identify tumor markers and bio-distribution of His_AP1_ferritin polypeptides. His_AP1_ferritin polypeptide was conjugated with a fluorescent dye (FNR 566) via chemical methods. Afterwards, unconjugated fluorescent dyes were separated through dialysis, and protein quantification was performed on remaining synthetic material. Mice with HeLa cell tumors were anesthetized with Isoflurane, a respiratory anesthetic, and the tail vein was injected via the tail vein with His_AP1_ferritin-FNR566 synthetic polypeptide (2 mg / kg). Fluorescence images were acquired using the IVIS spectrum in the main lane and images of the entire mouse were obtained from 2 hours to 24 hours after injection, and the bio-distribution and tumor markers of His_AP1_ferritin polypeptides were confirmed in real time.

As a result, as shown in FIG. 11, it was confirmed that the fluorescent dye was detected in the tumor site by 24 hours. Thus, it was confirmed that the fusion polypeptide of the present invention stably maintained its long-term activity in the body.

The present invention relates to a fusion polypeptide derived from human ferritin, and more particularly to a fusion polypeptide comprising a polypeptide fragment represented by SEQ ID NO: 1 and a human-derived ferritin monomer represented by SEQ ID NO: 6 in sequence. The fusion polypeptide of the present invention is capable of encapsulating an effective substance in the inside of a protein cage to form a protein cage and is highly effective in developing a diagnostic agent and a therapeutic agent for cancer and atherosclerosis by greatly improving the binding force of a polypeptide fragment with a specific molecule, It is highly likely to be used industrially.

<110> Kyungpook National University Industry-Academic Cooperation Foundation <120> Fusion polypeptide derived form human ferritin <130> NP13-0113 <150> GB 2012/0155222 <151> 2012-12-27 <160> 8 <170> Kopatentin 2.0 <210> 1 <211> 7 <212> PRT <213> Artificial Sequence <220> <223> AP1 <400> 1 Arg Lys Arg Leu Asp Arg Asn   1 5 <210> 2 <211> 81 <212> DNA <213> Artificial Sequence <220> <223> S1_AP1_ferritin_F <400> 2 gatccatatg catcatcacc accatcacgg cagccgaaaa cggctcgaca ggaacggcag 60 catgagctcc cagattcgtc a 81 <210> 3 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> S1_AP1_ferritin_R <400> 3 gatcctcgag ttagtcgtgc ttgagagtga gcc 33 <210> 4 <211> 54 <212> DNA <213> Artificial Sequence <220> <223> WT_ferritin_F <400> 4 gatccatatg catcatcacc accatcacgg cagcatgagc tcccagattc gtca 54 <210> 5 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> WT_ferritin_R <400> 5 gatcctcgag ttagtcgtgc ttgagagtga gcc 33 <210> 6 <211> 175 <212> PRT <213> Human ferritin light chain <400> 6 Met Ser Ser Gln Ile Arg Gln Asn Tyr Ser Thr Asp Val Glu Ala Ala   1 5 10 15 Val Asn Ser Leu Val Asn Leu Tyr Leu Gln Ala Ser Tyr Thr Tyr Leu              20 25 30 Ser Leu Gly Phe Tyr Phe Asp Arg Asp Asp Val Ala Leu Glu Gly Val          35 40 45 Ser His Phe Phe Arg Glu Leu Ala Glu Glu Lys Arg Glu Gly Tyr Glu      50 55 60 Arg Leu Leu Lys Met Gln Asn Gln Arg Gly Gly Arg Ala Leu Phe Gln  65 70 75 80 Asp Ile Lys Lys Pro Ala Glu Asp Glu Trp Gly Lys Thr Pro Asp Ala                  85 90 95 Met Lys Ala Met Ala Leu Glu Lys Lys Leu Asn Gln Ala Leu Leu             100 105 110 Asp Leu His Ala Leu Gly Ser Ala Arg Thr Asp Pro His Leu Cys Asp         115 120 125 Phe Leu Glu Thr His Phe Leu Asp Glu Glu Val Lys Leu Ile Lys Lys     130 135 140 Met Gly Asp His Leu Thr Asn Leu His Arg Leu Gly Gly Pro Glu Ala 145 150 155 160 Gly Leu Gly Glu Tyr Leu Phe Glu Arg Leu Thr Leu Lys His Asp                 165 170 175 <210> 7 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> His_AP1_ferritin <400> 7 catcatcacc accatcacgg cagccgaaaa cggctcgaca ggaacggcag c 51 <210> 8 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> His_ferritin <400> 8 catcatcacc accatcacgg cagc 24

Claims (12)

A fusion polypeptide represented by SEQ. ID. NO. 1 and a human derived ferritin monomer represented by SEQ ID NO. 6 are sequentially linked.
2. The fusion polypeptide of claim 1, wherein the fusion polypeptide comprises a histidine repeat tag in which 1 to 10 histidine repeats are preceded by a polypeptide fragment of SEQ ID NO: 1.
A ferritin protein comprising the fusion polypeptide of claim 1.
A polynucleotide encoding the fusion polypeptide of claim 1.
An expression vector comprising the polynucleotide of claim 4.
A transformant transformed with the expression vector of claim 5.
A protein cage comprising the fusion polypeptide of claim 1.
A composition for diagnosing cancer or arteriosclerosis comprising the fusion polypeptide of claim 1 as an active ingredient.
A cancer or atherosclerosis diagnostic kit comprising the fusion polypeptide of claim 1 as an active ingredient.
A protein nanoparticle comprising the fusion polypeptide of claim 1.
A protein chip comprising the protein nanoparticle of claim 10.
A composition for preventing or treating cancer or arteriosclerosis comprising the fusion polypeptide of claim 1 as an active ingredient.

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