KR20140062926A

KR20140062926A - Slow release carrier of green tea cells and the cosmetic composition containing the same

Info

Publication number: KR20140062926A
Application number: KR1020120129240A
Authority: KR
Inventors: 강현서; 강승현; 채병근; 한상훈
Original assignee: (주)아모레퍼시픽
Priority date: 2012-11-15
Filing date: 2012-11-15
Publication date: 2014-05-27
Also published as: KR102024571B1

Abstract

The present invention relates to a sustained-release green tea cell carrier and a cosmetic composition containing the same, and more particularly to a sustained-release green tea cell carrier and a cosmetic composition containing the sustained release green tea cell carrier, A green tea cell carrier and a cosmetic composition containing the same.

Description

[0001] The present invention relates to a sustained-release green tea cell carrier and a cosmetic composition containing the green tea cell carrier and the cosmetic composition containing the same,

Studies on sustained-release preparations that release drugs over a long period of time have been carried out variously from the past to the present. Particularly, research on sustained-release preparations using biodegradable polymers has recently been actively conducted. Polylactic acid (PLA), polyglycolic acid (PGA), polylactic-co-glycolic acid (PGA), which is a copolymer of these materials, and synthetic biodegradable polymers such as polyesters, poly lactic acid PLGA) have been developed and used. Polyesters are suitable for encapsulation of active materials but have a disadvantage in that their degradation rate in vivo is too slow. Hydrogels based on polyethylene glycol (PEG) and polysaccharides have the advantage of being excellent in biocompatibility and low toxicity, but they are disadvantageous in that they are difficult to be designed to stably maintain the active substance . These methods have problems such as difficulty in stabilization and poor biocompatibility.

Korea Patent Publication No. 1998-079285

The present inventors have been studying a carrier that can reduce the skin irritation of an active ingredient in a cosmetic product and exhibit uniform efficacy over a long period of time. However, the fact that a carrier prepared using plant cells, particularly green tea cells, And completed the present invention.

Accordingly, an object of the present invention is to provide a cosmetic composition using green tea cells as a sustained-release carrier.

In order to accomplish the above object, the present invention provides a cosmetic composition comprising green tea cells collected inside the active substance.

The green tea cell transporter used in the present invention is a sustained-release vehicle and is effective for stabilizing the active ingredient for a long period of time while being safe for the skin and exhibiting the efficacy of the active ingredient uniformly over a long period of time.

According to an embodiment of the present invention, there is provided a cosmetic composition comprising an active material stabilized in sustained-release green tea cells. In the present invention, it is preferable that the plant cells are prepared into a single cell through pretreatment through combination of enzymes. In the present invention, the enzyme may be any kind of enzyme that can be selected in the art. Available enzymes include, but are not limited to, glucosidase, xylosidase, xylanase, cellulose, galactosidease, pectinase, pectinase, naringinase, etc. The reaction using the enzyme is preferably performed under conditions of pH 3.0 to 8.0, reaction temperature 20 to 60 ° C, and reaction time 12 to 96 hours.

The term " sustained-release green tea cell transporter " of the present invention is prepared by separating cells of green tea leaves and collecting an active ingredient therein, and the active ingredient collected inside is gradually released without releasing it all at once. Quot; refers to an aqueous dispersion type particle prepared to exhibit water dispersibility.

In the present invention, 'active substance' refers to a substance that enhances or inhibits the function of a living body. According to one embodiment of the present invention, the active substance may be a hydrophilic or hydrophobic substance. The active substance of the present invention may be selected from the group consisting of green tea polyphenol (especially epigallocatechin gallate, EGCG), denier, retinol, retinyl palmitate, tocopherol, resveratrol, copper / plant extract, vitamin C and all derivatives derived therefrom , Kojic acid and all derivates derived therefrom, all peptide compounds by molecular weight, and caffeine. However, the present invention is not limited thereto.

According to one embodiment of the present invention, there is provided a method of preparing green tea cells comprising a stabilized active substance comprising the steps of:

(a) separating the green tea cells by treating the green tea leaves with an enzyme.

The green tea leaves are subjected to enzymatic treatment under the conditions of pH 3.0 to 8.0, preferably pH 6.5 to 7.5, reaction temperature 20 to 60 ° C, preferably 40 to 50 ° C, and reaction time 12 to 96 hours, preferably 72 hours. The enzyme may be selected from the group consisting of glucosidase, xylosidase, xylanase, cellulose, galactosidease, pectinase and naringinase (naringinase) can be used. The enzyme treated green tea leaves can be inactivated at high temperature, purified and dried to separate the cells from the green tea leaves.

(b) introducing the active substance into the green tea cell using osmotic pressure.

After the step (a) is completed, the dried green tea cells are put into the manufacturing equipment in which the active material is dissolved in a solvent such as water, ethanol, various polyols, etc., and the mixture is slowly stirred for 10 to 120 , Preferably 30 to 60 minutes. At this time, the active material is not limited thereto but is incorporated in an amount of 0.01 to 1.0% by weight based on the dry weight of the green tea cell carrier.

(c) obtaining green tea cells in which the active material is embedded.

Sodium or potassium salts are added to reverse the concentration gradient to remove moisture in plant cells through a reverse osmosis process. Thereafter, the green tea cells containing the active material are obtained through centrifugal separation, washing and drying processes.

(d) using green tea cells containing the active substance as a sustained-release preparation.

The green tea cells containing the active substance constitute a cosmetic composition to make a preparation suitable for the skin. At this time, the green tea cells containing the active material may be used alone or in combination with the drug delivery polymer to enhance the sustained release of the carrier. Polymers for drug delivery include, but are not limited to, poly lactic acid (PLA), poly glycolic acid (PGA), poly lactic-co-glycolic acid, Polyglycolic acid (PLGA), poly caprolactone (PCL), etc. may be used. The amount of the active ingredient may be 0.1 to 10.0% by weight based on the total weight of the composition containing green tea cells.

Also, the present invention provides a cosmetic composition comprising the sustained release green tea cell carrier in an amount of 0.00001 to 30.0% by weight based on the total weight of the composition. At this time, the green tea cell transporter may be directly blended with the cosmetic composition, or it may be blended after diluted with water or a solvent before blending.

The cosmetic composition containing the green tea cell carrier according to the present invention is not particularly limited in its formulation. For example, the cosmetic composition containing the green tea cell carrier according to the present invention has a formulation of a soft lotion, a nutritional lotion, a massage cream, a nutrition cream, a pack, Cosmetic composition, and may also be a transdermal dosage form such as lotion, ointment, gel, cream, patch or spray.

The green tea cell transporter according to the present invention exhibits a sustained release pattern of a drug or an active ingredient contained in cells, and is thus useful as a drug delivery vehicle for functional cosmetics.

Hereinafter, the present invention will be described in more detail by way of examples, but the present invention is not limited to these examples.

[Reference Example] Preparation of Examples and Comparative Examples

Examples 1 to 20 and Comparative Examples 1 to 30 were prepared with the compositions shown in Tables 1 to 5 below. Specific manufacturing methods are described below.

Example One 2 3 4 5 6 7 8 9 10 Green tea cell carrier 5 5 5 5 5 5 5 5 5 5 Bean cell transporter - - - - - - - - - - Epigallocatechin gallate 0.1 - - - - - - - - - Deni - 0.1 - - - - - - - - Retinol - - 0.1 - - - - - - - Retinol palmitate - - - 0.1 - - - - - - Tocopherol - - - - 0.1 - - - - - Rezveratrol - - - - - 0.1 - - - - Vitamin C - - - - - - 0.1 - - - AA2G - - - - - - - 0.1 - - Kojic acid - - - - - - - - 0.1 - Caffeine - - - - - - - - - 0.1 Polyglycolic acid - - - - - - - - - - Purified water Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

Example 11 12 13 14 15 16 17 18 19 20 Green tea cell carrier 5 5 5 5 5 5 5 5 5 5 Bean cell transporter - - - - - - - - - - Epigallocatechin gallate 0.1 - - - - - - - - - Deni - 0.1 - - - - - - - - Retinol - - 0.1 - - - - - - - Retinol palmitate - - - 0.1 - - - - - - Tocopherol - - - - 0.1 - - - - - Rezveratrol - - - - - 0.1 - - - - Vitamin C - - - - - - 0.1 - - - AA2G - - - - - - - 0.1 - - Kojic acid - - - - - - - - 0.1 - Caffeine - - - - - - - - - 0.1 Polyglycolic acid 5 5 5 5 5 5 5 5 5 5 Purified water Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

Comparative Example One 2 3 4 5 6 7 8 9 10 Green tea cell carrier Bean cell transporter 5 5 5 5 5 5 5 5 5 5 Epigallocatechin gallate 0.1 - - - - - - - - - Deni - 0.1 - - - - - - - - Retinol - - 0.1 - - - - - - - Retinol palmitate - - - 0.1 - - - - - - Tocopherol - - - - 0.1 - - - - - Rezveratrol - - - - - 0.1 - - - - Vitamin C - - - - - - 0.1 - - - AA2G - - - - - - - 0.1 - - Kojic acid - - - - - - - - 0.1 - Caffeine - - - - - - - - - 0.1 Polyglycolic acid - - - - - - - - - - Purified water Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

Comparative Example 11 12 13 14 15 16 17 18 19 20 Green tea cell carrier - - - - - - - - - - Bean cell transporter 5 5 5 5 5 5 5 5 5 5 Epigallocatechin gallate 0.1 - - - - - - - - - Deni - 0.1 - - - - - - - - Retinol - - 0.1 - - - - - - - Retinol palmitate - - - 0.1 - - - - - - Tocopherol - - - - 0.1 - - - - - Rezveratrol - - - - - 0.1 - - - - Vitamin C - - - - - - 0.1 - - - AA2G - - - - - - - 0.1 - - Kojic acid - - - - - - - - 0.1 - Caffeine - - - - - - - - - 0.1 Polyglycolic acid 5 5 5 5 5 5 5 5 5 5 Purified water Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

Comparative Example 21 22 23 24 25 26 27 28 29 30 Green tea cell carrier - - - - - - - - - - Bean cell transporter - - - - - - - - - - Epigallocatechin gallate
(Intracellular inclusion) 0.1 - - - - - - - - - Deni
(Intracellular inclusion) - 0.1 - - - - - - - - Retinol - - 0.1 - - - - - - - Retinol palmitate - - - 0.1 - - - - - - Tocopherol - - - - 0.1 - - - - - Rezveratrol - - - - - 0.1 - - - - Vitamin C - - - - - - 0.1 - - - AA2G - - - - - - - 0.1 - - Kojic acid - - - - - - - - 0.1 - Caffeine - - - - - - - - - 0.1 Polyglycolic acid 5 5 5 5 5 5 5 5 5 5 Purified water Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

1. Preparation of sustained-release green tea cell carriers (Examples 1 to 10)

Green tea leaves were treated with cellulase under the conditions of pH 7.0, reaction temperature 45 ° C and reaction time 72 hours, inactivated at 121 ° C, purified and dried to isolate green tea cells.

Each of the active materials was dissolved in 5% of water, and the mixture was stirred at 800 rpm and room temperature for 1 hour using a propeller mixer (BL3000, Heidon, JPN). Then, sodium chloride (sodium chloride) was added at a concentration of 2% by weight to induce reverse osmosis for 1 hour to remove moisture from the green tea cells. Then, a green tea cell carrier in which 2.0 wt% of the active substance was incorporated was obtained through centrifugal separation, washing and drying processes.

Then, 94.9 ml of water was added to each of 5.1 g of the sustained-release green tea cell carrier thus obtained to prepare Examples 1 to 10.

2. Preparation of a polymer-sustained-release green tea cell complex carrier (Examples 11 to 20)

5 g of polyglycolic acid and 89.9 ml of water were added to 5.1 g of each of the sustained-release green tea cell carriers prepared in the above 1. to hydrate them, and then Examples 11 to 20 were prepared.

3. Preparation of sustained-release soybean cell transporter (Comparative Examples 1 to 10)

The bean cell transporter was prepared in the same manner as above except that soybean (black tea) was used instead of green tea leaf.

4. Preparation of a polymer-sustained-release soybean cell complex carrier (Comparative Examples 11 to 20)

5 g of polyglycolic acid and 89.9 ml of water were added to 5.1 g of each of the sustained-release soybean cell transporter prepared in the above-mentioned 3., followed by hydration, and Comparative Examples 11 to 20 were prepared.

5. Preparation of a simple polymeric hydrogel carrier (Comparative Examples 21 to 30)

0.1 g of each active substance and 5 g of polyglycolic acid were dissolved in water to prepare a simple polymer hydrogel carrier Comparative Examples 21 to 30.

[Test Example 1] Drug release experiment

The carriers prepared in Examples 1 to 4 were placed in a dialysis bag (MW 10000, spectra / por, spectrum labs), and both sides were clogged with a closure, followed by releasing test in immersed water bath (37 ° C) in distilled water. As a comparative example, a simple polymer hydrogel carrier prepared by dissolving 0.1 weight% of epigallocatechin gallate or decane and 5.0 weight% of polyglycolic acid in 94.9 weight% of distilled water was used in the same manner as in Examples 1 to 4 Emission test. The drug released from the aqueous solution of the outer membrane of the dialysis membrane was measured by high performance liquid chromatography (Hewlett Packard) at regular intervals. The results are shown in Table 6 below.

Amount of release of active ingredient (%) over time 0 hours 2 hours 4 hours 6 hours 8 hours 12 hours 16 hours 20 hours 24 hours Example 1 5.5 23.5 43.2 54.3 64 82.1 93.5 99.8 99.8 Example 2 10.1 23 40 53 68 91 94.9 99.9 99.9 Example 3 9.8 42.4 64.3 72.5 88.8 96.2 99.9 100.1 100.1 Example 4 10.2 43.5 66.4 72.5 89.2 97.3 99.8 99.9 99.9 Example 5 11.2 43.1 65.5 73.4 85.9 92.3 98.4 100 100.1 Example 6 9.4 39.8 59.8 64.3 82.3 94.8 98.2 99.5 100 Example 7 8.2 38.4 54.5 63 79.9 89.8 94.5 98.3 99.9 Example 8 11.8 45 53 69.9 87.4 95.4 97.7 100.5 100.5 Example 9 6.9 39.5 57.3 65.9 70.3 88.3 96.9 99.7 101.3 Example 10 10.5 39.9 65.5 71.3 87.3 94.8 98.2 99.8 100 Example 11 3.1 6.9 10.1 17.3 24.9 49.8 72.4 87.5 99.3 Example 12 7.7 15 17.9 25.4 41.3 59.3 71.4 92.1 100.1 Example 13 4.3 13.4 20.5 41.3 51.5 62.9 88.3 97.8 99.8 Example 14 3.5 8.4 11.1 18.4 35 54.4 68.4 90.1 98.5 Example 15 3.8 7.9 10.9 18.9 33.9 53.2 70.2 88.8 99.7 Example 16 7.5 11.2 12 23.5 42.3 51 71.5 92.2 100.5 Example 17 2.4 9.9 13.2 19.9 41 48.8 78.8 91.8 99.5 Example 18 3.5 10.9 14.5 24.5 45 49.9 70 88.9 99.9 Example 19 4 8.3 12 22.3 35.4 53.4 69.9 89.2 99.8 Example 20 9.7 12.5 18.8 33.4 39.9 60.5 73.4 90 100.2 Comparative Example 1 10.4 42.4 69.9 73.2 89.2 98.7 99.9 100.0 100.0 Comparative Example 2 11.2 43.5 65.4 70.5 88.8 99.2 99.8 99.9 100.0 Comparative Example 3 12.3 45.1 68.5 73.4 90.5 97.5 99.5 99.9 99.9 Comparative Example 4 10.1 43.2 65.4 72.3 92.3 99.4 99.9 100.3 100.9 Comparative Example 5 13.3 45.4 70.0 80.1 93.4 99.5 99.8 99.8 99.8 Comparative Example 6 10.4 39.5 58.9 74.5 88.5 93.9 99.8 99.8 100 Comparative Example 7 10.9 45.4 61.5 73.9 89 95.5 99.8 100 100 Comparative Example 8 11.2 48.8 69.1 75.4 92.8 99.3 99.8 99.9 99.9 Comparative Example 9 12.4 49.5 68.8 75.4 90.5 92.5 99.5 100.1 100.5 Comparative Example 10 11.8 43.4 65.9 73.2 90.2 95.8 98.8 100.5 101.4 Comparative Example 11 5.5 7.3 10.8 18.2 34.2 58.4 69.3 96.4 100.1 Comparative Example 12 10.2 15.4 21.9 42 53.9 63 89.5 99.8 100.2 Comparative Example 13 8.2 16.4 23.3 42 52 64.9 90.1 99.9 99.9 Comparative Example 14 4.5 8.8 11.2 19.9 35.4 60.2 71.3 95 99.8 Comparative Example 15 6.9 10.5 15.4 21.2 38.8 59.9 70.5 90.1 102.1 Comparative Example 16 10.2 23.5 36.5 50.2 60.1 69.8 92 94.5 99.9 Comparative Example 17 11.4 21.4 34 48.7 59.2 64.9 91.4 97.9 99.9 Comparative Example 18 9.5 17.8 25.4 39.4 58.8 68.9 72 89.9 100 Comparative Example 19 8.2 13.5 15.3 32 44.4 60 70.3 89.9 99.9 Comparative Example 20 10.1 20.5 33.3 45.8 54.9 62.3 89.8 99 99.9 Comparative Example 21 11.0 42.8 69.9 75.5 80.3 92.9 99.6 99.6 99.7 Comparative Example 22 12.4 46.5 69.0 79.9 85 98.9 99.5 99.5 99.9 Comparative Example 23 13.2 45.3 66.4 74.2 89.9 96.8 98.8 99.2 99.7 Comparative Example 24 11 44.5 68.8 79.3 88 98.2 99.8 100.3 100.9 Comparative Example 25 12.4 39.4 72.5 83.8 90.5 96.1 96.2 98 99.3 Comparative Example 26 13 40.1 73.3 83.5 92.3 95.5 95.8 97.9 98.7 Comparative Example 27 11 45.8 69.2 78 89.3 93.4 98.8 99.4 101.3 Comparative Example 28 11.2 43.9 65.5 78.1 89.1 95.5 97.8 100.1 100.9 Comparative Example 29 12.5 49.6 69.5 79.9 89.9 93.1 98.9 99.9 99.9 Comparative Example 30 14.7 49.9 66 73.5 83.4 99.3 99.3 99.8 100.1

As shown in Table 6, the sustained-release green tea cell transporter exhibits a slightly enhanced sustained release property compared to the simple polymer transporter or the soy cell transporter. In addition, in the case of the polymer-sustained-release green tea cell complex, the sustained release property is remarkably enhanced as compared with the conventional simple polymer carrier, the soybean cell carrier and the polymer-soybean cell carrier.

Claims

A cosmetic composition comprising a green tea cell carrier comprising an active substance.

The cosmetic composition according to claim 1, wherein the green tea cell carrier is used together with a polymer.

The cosmetic composition according to claim 2, wherein the polymer is at least one selected from the group consisting of polylactic acid (PLA), polyglycolic acid (PGA), polylactic acid-glycolic acid (PLGA) and polycaprolactone (PCL).

The cosmetic composition according to claim 3, wherein the polymer is used in an amount of 0.1 to 10.0% by weight based on the total weight of the composition.

The cosmetic composition according to claim 1, wherein the active material is a hydrophilic or hydrophobic substance.

6. The composition of claim 5, wherein the active substance is selected from the group consisting of green tea polyphenols, denier, retinol, retinyl palmitate, tocopherol, resveratrol, animal or plant extract, vitamin C, kojic acid, .

(a) isolating green tea cells by treating green tea leaves with an enzyme;
(b) incorporating the active substance into the green tea cell using osmotic pressure;
(c) obtaining green tea cells in which the active material is embedded; And
(d) using green tea cells containing an active substance as a sustained-release preparation;
Wherein the active substance is entrapped in the interior of the green tea cell carrier.

The method of claim 7, wherein the enzyme used in step (a) is selected from the group consisting of glucosidase, xylosidase, xylanase, cellulose, galactosidease, , Pectinase, and naringinase. The method of producing a sustained-release green tea cell vehicle according to claim 1,