KR20140019656A - Multi type scaffold manufacturing device and scaffold using the same - Google Patents

Abstract

The present invention relates to an integrated apparatus for manufacturing a cell culture scaffold and a cell culture scaffold manufactured using the same, and more particularly, to an integrated apparatus for manufacturing a cell culture scaffold and a cell culture scaffold manufactured using the same, formed by comprising: a first spraying means for flowing in a solution stored in a first solution storage unit by air pressure into a screw unit having a screw thread, and then make the solution sprayed into a first nozzle unit by rotating the screw unit; and a second spraying means including a second nozzle unit in which the solution supplied from a second solution storage unit is sprayed, wherein, if a solution consisting of a hydrogel with a cell is stored in the first solution storage unit and a solution consisting of biocompatible materials is stored in the second solution storage unit and the solutions are alternately sprayed, cells can be uniformly cultured over the entire area of the cell culture scaffold as well as the cell culture scaffold formed by being sprayed by the second solution storage unit can reinforce the strength of the entire cell culture scaffold.

Description

TECHNICAL FIELD [0001] The present invention relates to a device for manufacturing a fusion cell type support and a cell culture support using the same,

More particularly, the present invention relates to a device for manufacturing a fusion cell culture support and a cell culture support manufactured using the same. More specifically, the solution stored in the first solution storage part is pneumatically injected into a screw part having a screw thread, And a second nozzle unit including a second nozzle unit through which the solution supplied from the second solution storage unit is injected, wherein a solution composed of a hydrogel and cells is formed on the first nozzle unit, When the solution stored in the first solution storage part and composed of the biocompatible polymer is stored in the second solution storage part and alternately injected, not only the cells can be cultured evenly over the whole area of the cell culture support, A cell culture support formed by spraying by a storage part can reinforce the strength of the entire cell culture support, and The present invention relates to cell culture support manufactured by the.

Tissue engineering is a technique in which a tissue is taken from the body of a patient and the cells are separated from the tissue. The separated cells are then expanded by the necessary amount by culturing and planted in a porous biodegradable polymer scaffold. And then transplanting the hybrid cell culture scaffold back into the human body.

After transplantation, most tissues and organs receive oxygen and nutrients by the diffusion of body fluid until new blood vessels are formed, but when blood vessels enter the body and supply blood, the cells proliferate and differentiate to form new tissues and organs. It is to apply the technique that forms and the polymer support is decomposed and disappeared in the meantime.

Therefore, for such tissue engineering studies, it is important to first produce cell culture supports similar to living tissue.

A cell culture apparatus has also been disclosed in Korean Patent Publication No. 2010-0011175 (name: cell culture apparatus and control method thereof).

Meanwhile, as shown in FIG. 1, a cell culture using a conventional cell culture support is generally performed by seeding cells (C) separately on a previously prepared cell culture support (S).

However, this method has a problem in that when the cell culture supports of various layers are laminated, the cells scattered from the upper side are most scattered on the cell culture support located on the uppermost side, and the amount reaching the cell culture support located in the inner layer is very small . In addition, there is a problem that it is difficult to spread the cells for each cell culture support on each layer in order to cultivate different kinds of cells.

Korean Patent Publication No. 2010-0011175 (published on Feb. 30, 2010, name: cell culture apparatus and control method thereof) Korean Patent Publication No. 2010-0083332 (published on July 22, 2011, entitled " Cell culture support support manufacturing apparatus and manufacturing method)

SUMMARY OF THE INVENTION The present invention has been conceived in order to solve the above-mentioned problems, and it is an object of the present invention to provide an apparatus and a method for injecting a solution stored in a first solution storing part by pneumatic pressure into a screw part having a screw thread, And a second injection unit including a second nozzle unit through which the solution supplied from the second solution storage unit is injected, wherein a solution composed of the hydrogel and the cells is formed in the first solution storage unit And the solution composed of the biocompatible polymer is stored in the second solution storage part and alternately injected so that the cells can be uniformly spread over the whole area of the cell culture support and the solution formed by spraying by the second solution storage part A device for manufacturing a fusion type cell culture support in which a cell culture support can reinforce the strength of an entire cell culture support, and a cell culture To provide a delay.

The apparatus for manufacturing a cell culture support according to the present invention comprises: a first solution storage part 110 for storing a solution composed of a cell and a biocompatible material; A second solution storage part 210 in which a solution composed of a biocompatible polymer is stored; A first pressurizing unit 130 for applying a pneumatic pressure to the solution stored in the first solution storage unit 110; an inlet 151 through which the solution stored in the solution storage unit flows by pneumatic pressure; And a threaded portion 161 rotatably mounted within the first outer housing 140 and communicating with the inlet 151 and the outlet 152. The first outer housing 140 includes a first outer housing 140, A first injection unit 100 formed to include a first nozzle unit 120 connected to the discharge port 152 and sprayed with a solution; A second injection unit 200 including a second nozzle unit 220 through which the solution supplied from the second solution storage unit 210 is injected; A collecting unit 300 provided below the first injecting unit 100 and the second injecting unit 200 to collect the solution injected through the first nozzle unit 120 and the second nozzle unit 220, ; A jet position adjusting unit 400 for adjusting the jet position by moving the first jetting means 100 and the second jetting means 200 or the collecting unit 300 in the x, y and z directions; And a control unit controlling the injection position adjusting unit (400). And is formed to include the first and second electrodes.

In addition, the second injecting unit according to an embodiment of the present invention may include a second pressurizing unit that applies pneumatic pressure to the solution stored in the second solution storage unit 210 and injects the solution into the second nozzle unit 220, (230); And heating means (240) provided with a hot wire along the outer circumferential surface of the second solution storage portion (210) to melt and inject the biocompatible polymer stored in the second solution storage portion (210). It may be formed to include.

In addition, the apparatus for producing a fusion cell culture support 1 according to an embodiment of the present invention, the first injection means 100 and the second injection means 200 are spaced apart from each other, the control unit by the The first nozzle unit 120 and the second nozzle unit 220 are sequentially positioned at a predetermined position of the collection table so that the solution is injected, and the first nozzle unit 120 and the second nozzle unit 220 are The solution sprayed through may be alternately sprayed layer by layer, or alternately sprayed every row of a predetermined interval.

The first injection means 100 according to an embodiment of the present invention includes a motor unit 180 mounted inside the first outer housing 140 to rotate the screw unit 160 .

In addition, a plurality of the first solution storage parts 110 may be formed in the first injection device 100 according to an embodiment of the present invention.

In addition, the first solution storage part 110 according to an embodiment of the present invention includes a first solution solution storage part 111 and a first solution solution storage part 112, -2 solution storage portion 112 may contain a curing agent or a solution containing the cells other than the 1-1 solution storage portion 111.

In addition, the first solution storage part 110 according to an embodiment of the present invention may further include a 1-3 solution storage part 113, and the 1-3 solution storage part 113 may contain a hardening agent Alternatively, a solution containing cells different from the first solution solution storage part 111 and the first solution solution storage part 112 may be stored.

In addition, the biocompatible material contained in the solution stored in the first solution storage part 110 according to an embodiment of the present invention may be a hydrogel.

In addition, the solution stored in the second solution storage part 210 according to an embodiment of the present invention may be a biocompatible polymer having a strength higher than that of the hydrogel in a cured state.

Also, the apparatus 1 for preparing a cell culture support for supporting a fusion type cell according to an embodiment of the present invention may include heating means 230 for controlling the temperature of the solution stored in the second solution storage unit 210.

The cell culture support of the present invention is produced using the apparatus (1) for producing a fusion cell culture support.

The apparatus for manufacturing a fusion type cell culture support according to the present invention comprises first injecting means for introducing a solution stored in a first solution storage portion by pneumatic pressure into a screw portion having a screw thread and then rotating the screw portion to inject the solution into the first nozzle portion, And a second nozzle unit including a second nozzle unit through which the solution supplied from the second solution storage unit is injected. Therefore, the solution can be uniformly injected and the injection amount of the solution can be easily controlled.

In addition, in the apparatus for producing a cell culture support of the present invention and the cell culture support prepared using the same, a solution composed of a hydrogel and cells is stored in a first solution storage part and a solution composed of a biocompatible polymer is stored in a second solution storage part The cell culture support formed by spraying by the second solution storage part can reinforce the strength of the whole cell culture support, And a cell culture support prepared using the same.

In other words, the apparatus for producing a cell culture support of the present invention and the cell culture support manufactured using the apparatus of the present invention are sprayed in a mixed state with a solution composed of a cell and a biocompatible material to produce a cell culture support, The cells can be cultured evenly over the whole area of the cell culture support as compared with the conventional method in which the cells are sprayed and transplanted. When a plurality of cell culture supports are stacked, the cells can be uniformly distributed in each layer, It is also suitable for culturing cells.

In addition, the apparatus for manufacturing a fusion type cell culture support of the present invention may include a plurality of first solution storage parts and a second solution storage part for storing solutions containing different cells, so that various types of cell culture support can be easily It is advantageous that it can be manufactured.

1 is a schematic view showing a conventional cell culture method.
2 is a perspective view showing an apparatus for producing a fusion cell culture support according to the present invention.
3 is a perspective view illustrating an embodiment of a first injecting unit provided in an apparatus for producing a cell culture support for fusion type according to the present invention;
Fig. 4 is a sectional view showing the first injection means of Fig. 3; Fig.
FIG. 5 is a perspective view showing still another embodiment of the first injecting unit provided in the apparatus for producing a cell culture support for fusion type according to the present invention; FIG.
6 is a cross-sectional view showing one embodiment of a second injecting unit provided in an apparatus for producing a cell culture support for fusion type according to the present invention.
7 is a view showing an example of a cell culture support prepared using the apparatus for producing a fusion cell culture support according to the present invention.
FIG. 8 shows another embodiment of the cell culture support prepared using the apparatus for producing a cell culture support according to the present invention.
9 is a view showing still another embodiment of a cell culture support prepared using the apparatus for producing a cell culture support according to the present invention.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, an apparatus for producing a fusion type cell culture support of the present invention as described above will be described in detail with reference to the accompanying drawings.

FIG. 1 is a schematic view showing a conventional cell culturing method, FIG. 2 is a perspective view showing a device for producing a cell type support for fusion cell culture according to the present invention, FIG. 3 is a cross- FIG. 4 is a cross-sectional view showing the first injecting means of FIG. 3, and FIG. 5 is a cross-sectional view of the first injecting means provided in the apparatus for producing a cell- 6 is a cross-sectional view illustrating an embodiment of a second injecting unit provided in an apparatus for producing a cell type support for a fusion cell according to the present invention, and Fig. 7 is a cross- 8 is a view showing another embodiment of the cell culture support prepared by using the apparatus for producing a fusion type cell culture support according to the present invention And FIG. 9 is another embodiment of the cell culture support prepared using the apparatus for preparing a cell culture support according to the present invention.

Example 1

Example 1 relates to an apparatus for manufacturing a cell culture support according to the present invention.

2, the apparatus for producing a cell culture support support of the present invention includes a first solution storage part 110, a second solution storage part 210, a first injection device 100, (200), a collecting unit (300), a jetting position adjusting unit (400), and a control unit.

In the first solution storage part 110, a solution or a curing agent composed of cells and a biocompatible material is stored. In the second solution storage part 210, a solution composed of a biocompatible polymer is stored.

At this time, the biocompatible material may be any one of a biocompatible polymer, a bioprotein, and a hydrogel.

Particularly, the injection means of the device for manufacturing a fusion type cell culture support of the present invention may include the first pressing means 130, the first outer housing 140, and the screw portion 160.

2 to 4, the first injecting unit 100 includes a first solution storage unit 110, a first pressurizing unit 110 for applying a pneumatic pressure to the solution stored in the first solution storage unit 110, (130). In this case, the first solution storage part 110 may be in the form of an empty cylinder, and the first pressing part 130 may be inserted into the first solution storage part 110 in the form of a piston, The solution stored in the first solution storage part 110 can be pushed out to the outside.

In addition, the first pressurizing means 130 may be of a type that pneumatically presses the empty cylinder, i.e., a form in which the air pushes the solution in the cylinder.

The first outer housing 140 includes an inlet 151 through which the solution stored in the first solution reservoir 110 flows by pneumatic pressure and an outlet 152 through which the solution is discharged, Is rotatably mounted in the first outer housing 140 and has a thread 161 communicating with the inlet 151 and the outlet 152.

The first injecting unit 100 may further include a pitch adjusting unit 170. The pitch adjusting unit 170 may be mounted on the outside of the first outer housing 140, So that the screw portion 160 can be manually rotated.

3 to 4, the first injection means 100 is configured such that the solution stored in the first solution storage portion 110 is pressed downward by the first pressing means 130, 151 and flows into the thread 161 of the screw 160 and the solution introduced into the screw 161 is discharged through the outlet 152 by the rotation of the screw 160.

The collecting unit 300 may collect the solution injected through the first nozzle unit 120 of the first injecting unit 100 or the second nozzle unit 220 of the second injecting unit 200, And is provided below the injection means 100 or the second injection means 200.

The collecting unit 300 may be in the form of a plate as shown in FIG. 2, and is not limited to a specific form, and various changes can be made in various ways.

The ejection position adjusting unit 400 moves the first ejecting unit 100 and the second ejecting unit 200 or the collecting unit 300 in the x, y and z directions so that the collecting unit 300 Is positioned below the first injection means (100) or below the second injection means (200).

2, the injection position regulating unit 400 controls the first and second injectors 100 and 200 to move in the z-axis direction a x-axis guide rail 420 and a y-axis guide rail (not shown) for moving the fixing bracket 410 in x and y axes, respectively, As shown in FIG.

In the apparatus for manufacturing a cell culture support support of the present invention, the injection position can be adjusted by moving the injection means by the injection position adjustment unit 400, but the injection means can be moved in at least one direction of x, y, And the collecting unit 300 may be moved in the remaining direction in which the injecting unit is moved to adjust the injection position.

The control unit is connected to the injection position adjusting unit 400 to control the injection position.

At this time, in the apparatus 1 for manufacturing a fusion-type cell culture support of the present invention, the first injection means 100 and the second injection means 200 are spaced apart from each other, and the predetermined position of the collection table is controlled by the control unit. The first nozzle unit 120 and the second nozzle unit 220 are sequentially positioned in order to spray the solution, but the solution sprayed through the first nozzle unit 120 and the second nozzle unit 220 Alternately sprayed in layers, or alternately sprayed in rows at regular intervals.

Meanwhile, in the apparatus for producing a cell culture supporter according to the present invention, the solution stored in the first solution storage part 110 is injected in a mixed state of the cells and the biocompatible material. In the mixed state, It is preferable that the biocompatible material is a hydrogel.

In this case, when the biocompatible material is a hydrogel, it is preferable that a solution in which the hydrogel and the cell are uniformly mixed is stored in the first solution storage part 110.

At this time, the amount of the solution can be controlled by manually discharging the solution according to the angle at which the pitch adjusting part 170 is rotated.

The first injecting unit 100 may further include a motor unit 180 mounted inside the first outer housing 140 to rotate the screw unit 160. Accordingly, The screw portion 160 can be automatically rotated according to the rotation of the motor portion 180. [

In this case, the motor unit 180 is connected to the control unit 500 so that the rotation speed and the rotation angle can be adjusted.

The first injecting unit 100 may include a rotating means adjusting unit 190 for selecting one of the pitch adjusting unit 170 and the motor unit 180 to rotate the screw unit 160 .

The rotating means adjusting portion 190 is a kind of switch which allows the screw portion 160 to be manually rotated by the pitch adjusting portion 170 or to be automatically rotated by the motor portion 180 do.

6, the second injecting unit 200 applies a pneumatic pressure to the solution stored in the second solution storing unit 210 and injects the second pressurizing force to inject the solution into the second nozzle unit 220, And a heating means 240 for heating and fusing the biocompatible polymer stored in the second solution storage portion 210 with a hot wire along the outer circumferential surface of the second solution storage portion 210 .

When the biocompatible polymer stored in the second solution storage part 210 is melted by the heating device 240 and becomes liquid, the second injecting device 200 injects the biocompatible polymer, So that the biocompatible polymer in a liquid state is sprayed to the collecting unit 300 through the second nozzle unit 220.

 Accordingly, the apparatus 1 for producing a cell culture support of the present invention and the cell culture support prepared therefrom are characterized in that a solution composed of a hydrogel and cells is stored in a first solution storage part 110 and is composed of a biocompatible polymer The solution is stored in the second solution storage part 210 and alternately injected, so that the cells can be cultured evenly over the whole area of the cell culture support.

In addition, in order to solve the problem that the cell culture support injected in the first solution storage part composed of the hydrogel and the cell alone has a low mechanical rigidity, the present invention provides a cell culture support formed by spraying by the second solution storage part 210 Lt; RTI ID = 0.0 > cell culture < / RTI > support.

In other words, even if a solution composed of a hydrogel and a cell is cured, the rigidity of the hydrogel is inferior due to the characteristics of the hydrogel. However, the present invention is applicable to a solution made of a biocompatible polymer having a stronger strength in a hardened state than a hydrogel 2 solution storage unit 210 and then sprayed through the second nozzle unit 220 to reinforce the strength of the whole cell culture support.

7, a cell culture support (a white part without a pattern) formed of a solution composed of a biocompatible polymer injected through the second nozzle part 220 and maintaining rigidity, and a support part There is shown a cell culture support in which a cell culture support in which the cells are uniformly distributed in the inside is alternately formed as a mixed solution of injected hydrogel and cells.

As described above, the apparatus (1) for producing a cell culture support according to the present invention and the cell culture support produced therefrom are sprayed in a mixed state with a solution composed of a cell and a biocompatible material to produce a cell culture support, Cells can be cultured evenly over the entire area of the cell culture support as compared to the conventional method in which cells are separately sprayed and transferred onto the cell culture support. When a plurality of cell culture supports are stacked, cells can be uniformly distributed in each layer , And is also suitable for culturing different cells by layer.

Example 2

Example 2 relates to another embodiment of the cell culture support manufacturing apparatus according to the present invention.

5, a plurality of first solution storage units 110 of the first injecting unit 100 may be formed in the apparatus for manufacturing a cell culture support support of the present invention.

At this time, the first solution storage part 110 may be disposed along the outer circumferential surface of the first outer housing 140, and may be the same as the number of the inlet 151 formed in the first outer housing 140 In consideration of the size of the inlet 151, the number is not limited as long as only a space in which the inlet 151 can be formed is secured.

The solution storage part includes a 1-1.sup.th solution storage part 111 and a 1-2.sup.th solution storage part 112. In the 1-2.sup.th solution storage part 112, A solution containing the cell 111 and other cells may be stored and the curing agent may be stored.

The first solution storage part 110 may further include a first solution solution storage part 113. The first solution solution storage part 113 may contain a curing agent or the first solution solution, The solution containing the cells different from the first solution storage part 111 and the first solution storage part 112 may be stored.

The hydrogel may be alginate, and the curing agent may be a calcium chloride (Cacl 2) solution. It is known that alginate can be mixed with cells at room temperature and is cured rapidly when mixed with calcium chloride (Cacl 2) solution.

At this time, in the apparatus for producing a cell culture support for supporting a fusion type cell according to the present invention, the hardening agent is stored in any one of the plurality of first solution storage portions 110, so that the hardening agent is sprayed onto the collection portion 300 on which the cell culture support- Thereby causing curing to occur.

Accordingly, the apparatus for producing a fusion type cell culture support of the present invention is a device for producing a cell culture support, which is a mixture of cells and a biocompatible material, As shown in FIG.

In other words, the apparatus for producing a cell culture support according to the present invention is capable of producing a cell culture support by mixing a cell and a solution composed of a biocompatible material in a mixed state to produce a cell culture support, As shown in FIG. 7, the cells can be uniformly distributed over the entire area of the cell culture support. When a plurality of cell culture supports are stacked as shown in FIG. 8, cells can be uniformly distributed in each layer, It is also suitable for culturing.

In addition, as shown in FIG. 9, the apparatus 1 for producing a cell culture support according to the present invention can allow different cells to be cultured on a cell culture support formed of a single layer.

For this purpose, the first injection means 100 is configured such that the solutions stored in the plurality of first solution storage portions 110 are simultaneously introduced into the screw portion 160 and mixed by the rotation of the screw portion 160, The mixed solution may be sprayed through the first nozzle unit 120 and the solution stored in the plurality of first solution storage units 110 may flow into the screw unit 160 separately, (Not shown).

Accordingly, when a plurality of first solution storage parts 110 are provided, the apparatus 1 for producing a cell culture support support of the present invention mixes solutions in the threads 161 of the screw part 160, The cells are sprayed at the same time or sequentially sprayed to cure the cells, thereby various types of cell culture supports can be easily manufactured.

In this case, the cell culture support shown in FIGS. 7 to 9 is prepared by mixing a solution composed of a cell and a biocompatible material, and a solution composed of a biocompatible polymer by alternating the first injecting means 100 and the second injecting means 200 So that the rigidity can be further reinforced.

It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. It goes without saying that various modifications can be made.

1: Fusion type cell culture support preparation apparatus
2: Cell culture supporter
100: first injection means
110: first solution storage part 111: first solution storage part
112: 1-2 solution storage part 113: 1-3 solution storage part
120: nozzle part
130: first pressing means 140: first outer housing
151: inlet 152: outlet
160: Screw section 161:
170: pitch adjusting section 180: motor section
190:
200: second injection means
210: second solution storage part 220: second nozzle part
230: second pressing means 240: heating means
300: collector
400: injection position adjusting section
410: Fixing bracket
420: X-axis guide rail
500:

Claims (10)

A first solution storage part 110 in which a solution composed of a cell and a biocompatible material is stored;
A second solution storage part 210 storing a solution in which the biocompatible polymer is melted;
A first pressurizing unit 130 for applying a pneumatic pressure to the solution stored in the first solution storage unit 110; an inlet 151 for pumping the solution stored in the first solution storage unit; A first outer housing 140 that includes an outlet 152 and a threaded portion 161 that is rotatably mounted within the first outer housing 140 and communicates with the inlet 151 and outlet 152 A first injecting means 100 including a screw portion 160 and a first nozzle portion 120 connected to the discharge port 152 to inject a solution;
A second injection unit 200 including a second nozzle unit 220 through which the solution supplied from the second solution storage unit 210 is injected;
A collecting unit 300 provided below the first injecting unit 100 and the second injecting unit 200 to collect the solution injected through the first nozzle unit 120 and the second nozzle unit 220, ;
A jet position adjusting unit 400 for adjusting the jet position by moving the first jetting means 100 and the second jetting means 200 or the collecting unit 300 in the x, y and z directions; And
A control unit for controlling the injection position adjusting unit 400; Wherein the cell culture support support is a cell culture support.
The method of claim 1,
The second injection means
Second pressurizing means (230) for applying pneumatic pressure to the solution stored in the second solution storage portion (210) and spraying the solution to the second nozzle portion (220); And
Heating means (240) provided with a hot wire along the outer circumferential surface of the second solution storage portion (210) to melt and spray the biocompatible polymer stored in the second solution storage portion (210); The cell culture support support of claim 1,
3. The method of claim 2,
The apparatus 1 for producing a cell culture support for a fusion type
The first injection means 100 and the second injection means 200 are disposed apart from each other,
Wherein the first nozzle unit 120 and the second nozzle unit 220 are sequentially positioned at a predetermined position of the collection table by the control unit so that the solution is injected,
Wherein the solutions sprayed through the first nozzle part (120) and the second nozzle part (220) are alternately sprayed in alternating layers or alternately sprayed in every interval of a predetermined interval.
The method of claim 1,
The first injection means (100)
And a motor unit (180) mounted inside the first outer housing (140) and rotating the screw unit (160).
The method of claim 1,
The first injection means (100)
Wherein the first solution storage part (110) is formed in a plurality of the first solution storage part (110).
6. The method of claim 5,
The first solution storage part 110
A first solution storage part 111 and a first solution storage part 112,
Wherein the solution containing the curing agent or the cells other than the solution 1-1 is stored in the solution 1-2 of the first solution 1-2.
The method according to claim 6,
The first solution storage part 110
The first to third solution storage part 113,
The solution containing the hardener or cells different from the first solution solution storage part 111 and the first solution solution storage part 112 is stored in the first solution solution storage part 113 Wherein the cell culture supporter is a cell culture support.
8. The method according to any one of claims 1 to 7,
Wherein the biocompatible material contained in the solution stored in the first solution storage part (110) is a hydrogel.
The method of claim 8,
The solution stored in the second solution storage part 210
Wherein the polymer is a biocompatible polymer having a strength higher than that of the hydrogel in a cured state.
Cell culture support prepared by using the apparatus for producing a fusion-type cell culture support according to claim 9.

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