KR20140005004A - A feed composition for poultry - Google Patents

Abstract

The present invention provides a feed composition for poultry containing vegetable probiotics and omega-3 fatty acid which is obtained by mixing 48-79.99 wt% of regular poultry feed, 20-50 wt% of omega-3 fatty acid, and 0.01-2.0 wt% of vegetable probiotics. The present invention additionally contains the vegetable probiotics, bamboo leaf powder, and Houttuynia cordata compare to the regular poultry feed for offering a control effect of weight gain of poultry, bacteria colony amount (microbial content), and pathogenic microorganism. The present invention also has a remarkable increasing effect on the quality of meat and on delaying the decomposition of the meat during a storage process. The present invention has functions of poultry stomach protection, inflammation removal from intestines, and cell recovery for maximizing the survival rate of the poultry.

Description

Feed composition for poultry containing plant probiotics and omega-3 fatty acids {A feed composition for poultry}

The present invention relates to a feed composition for poultry containing plant probiotics and omega-3 fatty acids, more preferably omega-3 fatty acids in plant probiotics containing L. plantarum and L. salivarius obtained by fermentation of fruits. By providing a feed containing them, they can be substituted for the prevention and treatment of poultry diseases without the use of antibiotics, and can promote the growth of poultry.

As is generally known, in the animal feed of poultry such as chickens and ducks, antibiotics and the like have been added to the feed for the purpose of increasing body weight, improving breeding efficiency, and preventing bacterial diarrhea. However, in order to avoid residues in meat and eggs, the use of antibiotics and the like has been restricted by the law on feed stability (law on securing feed stability and improving quality).

Therefore, there is a need for a substance which achieves the above object in place of antibiotics, and the like, and probiotics have been noted as such substances.

Such probiotics include lactobacillus (Lactobacillus spp.), Lactobacillus (such as Streptococcus Faecalis), Bifidobacterium] or lactic acid bacterium (such as Clostridium butyriccomb). butyricum)] is a commercially available probiotic with active ingredients [Kamura Makoto: Livestock Research Vol. 33, No. 1, 31-36 (1979)].

Also, as a probiotic of the genus Bacillus, natto BN strain [B.natto is classified taxonomically as B.subtilis; Bari's manual of systematic system bacteria Vol. II (1986)] preparation of spores (trade name "Glogen", manufactured by Ezai Co., Ltd.) [manufactured by Kimura: published in front, Ohta Deruo; Miso Science and Technology No. 293 (August 7, 1978), 1-9 et al.] And B. toyoi [B.toyoi is classified as B.cereus by taxonomy] Spore preparation (trade name "Toyoserine") and Asahi Kasei Kogyo Co., Ltd. are known, and together have the above-described target effect.

On the other hand, administration of a probiotic containing Bacillus subtilis C-3102 (Microtechnologies No. 1096) to mammals, poultry, fish and other animals results in an increase in weight gain and feed efficiency. It is known to obtain (Japanese Patent Laid-Open No. 63-209580), and a compounded feed for piglets containing Bacillus and Subtilis C-3102 as an active ingredient is known (Japanese Patent Laid-Open No. 62-232343).

However, it is not known that preparations containing live organisms of C-3102 strain have the effect of reducing the intestinal Salmonella bacteria of poultry such as chicken when ingested by mixing them in feed.

The present invention has been made to solve the above problems, it is an object of the intestinal bacteria and pathogenic microorganisms can be suppressed by mixing the plant probiotics and omega 3 fatty acids are added to the feed for poultry.

In addition, it is possible to improve the weight and health of poultry without fungicides and antibiotics by further mixing vegetable live bacteria and omega-3 fatty acids in general poultry feed, and furthermore, to promote the growth promotion of poultry. It is done.

In addition, the blend of plant probiotics and omega-3 fatty acids is aimed at the efficiency of feed and to prevent and treat diseases.

Feed composition for poultry containing the plant live bacteria and omega-3 fatty acids of the present invention having the above object is 20 to 50% by weight of the omega-3 family fatty acids and 48 to 79.99% by weight of general poultry feed, 0, It is characterized in that the composition by mixing 01 to 2.0% by weight.

In addition, the present invention is characterized in that the plant live bacteria L. plantarum and L. salivarius strains obtained by the fermentation of vegetables and fruits.

The present invention, because it contains more plant live bacteria and omega-3 fatty acids than the general poultry feed, it has the effect of inhibiting the weight gain, bacterial colonies (pathogens), pathogenic microorganisms of poultry, meat quality and meat during storage Deterioration of the deterioration can show an excellent synergistic effect, and it is a very useful invention that can maximize the survival rate of poultry because of the functions of gastrointestinal protection of poultry, removal of inflammation in the intestine and cell regeneration.

1 is a photograph for showing the feed ingredient amount of the feed composition for poultry containing the plant live bacteria of the present invention.
Figure 2 is a photograph of the test report of duck meat fed the present invention poultry feed composition.
Figure 3 is a photograph of the test report of duck meat fed the present invention poultry feed composition.

Hereinafter, the feed composition for poultry containing plant live bacteria and omega-3 fatty acids according to an embodiment of the present invention will be described in more detail.

In order to prepare the feed for the poultry of the present invention, 48 to 79.99 wt% of general poultry feed is mixed with 20 to 50 wt% of omega-3 fatty acids and 0,01 to 2.0 wt% of plant probiotics. At this time, the plant probiotic L. plantarum and L. salivarius strains obtained by fermentation of vegetables and fruits are used.

The omega-3 fatty acid refers to an essential fatty acid having a double bond to the third carbon from the end of the carbon side chain among unsaturated fatty acids, and this essential fatty acid is a component of the tissue of the cell to promote metabolism.

The source of the omega-3 fatty acids include rapeseed seed, fish meal, flaxseed, flax seed, perilla seed and the like.

In addition, the plant probiotics used in the present invention will be L. plantarum or L. salivarius strain obtained by the fermentation of vegetables and fruits, characterized by living microorganisms, and the results of investigations on poultry in the prevention and treatment of poultry diseases It has been shown to have a very good effect.

The live bacteria used in the present invention are composed of strains of L. acidophilus, L.casei, L. helvelicus, L. lactis, L. salivarius, and L. plantarum, all of which are originally obtained from intestinal strains. . The reason for selecting Lactobacillus bacillus is because it contains Salmonella typhimurium, Staphylococcus aureus, Escherichia coli, Clostridium perfringens, and Clostridium difficile, and inhibits in vitro growth of intestinal pathogens and is very effective in gastrointestinal disorders of animals. .

Experimental results of the intestinal pathogens can be confirmed that L. salivarius and L. plantarum strains are very useful as poultry for plant live bacteria, in particular, the ability to inhibit enteritis bacteria, adhesion efficiency to the intestinal mucosa, bile acid resistance, etc. .

In particular, when L. salivarius and L. plantarum strains were added to oral gavage of poultry, and added to feed, drinking water was shown to prevent the formation of gastrointestinal enterococci colonies of poultry, and reduced Enterobacteriaceae colonies in poultry dung. It was confirmed to make.

The above experimental method is as follows.

1) Materials

From the local hatcheries, young broilers were produced with a high yield of 45 days of hatching. All poultry is vaccinated with New Castle and Bronchitis sprays in hatcheries and no other vaccinations. Each cage contains 30 Feather-sexed poultry in 15 cages (1..5 x 4.3M). Each cage was equipped with a wooden padded rice (sawdust) on a concrete floor and a single tube feed and nipple drinking water supply line to provide ad libitum to feed and drinking water access. The room temperature was controlled by raising and lowering the heat provided by the incubator lamp and the site curtain. The broiler is fed corn-bean basic commercial feed in a mesh manner. Formulate basic feeds that meet or exceed the requirements of the Starter and Grower.

Young poultry randomly appoint one of the three feed treatments. The crude classification is as follows: a basic feed containing 750 g / mtons of coban60 (an antiparasitic inhibitor) and a basal feed containing 62.5 g / mton of bacitracin (antibiotic) or no pesticides and antibiotics. Basic feed supplemented with 0.1% or 0.2% of plant probiotics was used.

Experimental poultry was observed twice daily and death was removed and body weights recorded. Non-economics with severe abnormalities in the legs were removed, weight recorded and euthanized. Death and leg abnormalities were recorded and recorded at the end of the study to determine mortality and leg abnormalities.

Poultry was weighed on days 21 and 42 and weight gain and feed conversions were calculated.

Feed conversion is determined by the difference between the weight put into the feeder and the remaining weight at 21 and 42 days. Body weight checks for mortality and leg failure broilers are used to control feed consumption. The University of Georgia Animal Care and Use Committee approved all animal procedures and plans used in this experiment.

2) Calculation of microorganisms of plant live bacteria

25 g vegetable probiotic The specimen and 222 ml 0.1 wt% peptone water were mixed at high speed for 2 minutes using a Waring Laboratory Blender and continuously dilute with 0.1 wt% peptone water.

These dilutions were calculated for Lactobacillus bacilli and yeast in MRS Lactic Acid medium or Acidified Potato Dextrose medium, respectively. Inoculation MRS Lactic Acid medium was incubated for 48 hours at 35 ° C. in a Coy Controlled Environment Chamber. Inoculation Acidified Potato Dextrose medium is incubated for 3 days at 25 ℃. Colonies on the media were counted and morphologically apparent colonies were removed from the media for identification by Sherlock Microbial Identification.

3) Immunoassay Procedure

On day 42 take two poultry from each cage and orally add 1 mL of suspension containing approximately 5X10 CFU of C.jejuni 2b. C. jejuni 2b isolates were recovered from broiler washes commercially treated by the Poultry Processing and Meat Quality Unit at the Russell Research Center in Athens, GA.

4) Sample procedure

I feed from each cage 12 hours before treatment. At 56 hours, two immunized broilers and three unimmunized broilers were transported to a pilot prosessing plant in transport cages. The broilers were processed in turn, according to our number, and each leg band was attached to the body to identify the order of treatment. Each 5 broilers (3 non-immunized and 2 immunized) were stunned with 12V pulsed DC and bleeded for 120 seconds and scalded in water (48,53 and 57C) for a total of 120 seconds. . A single picker removes the body's hair but does not remove the epidermis.

To minimize leakage from the digestive tract during the entire body wash collection, the picker is removed and the anus of each corpse is inserted into the excretory cavity with a cotton tampon to block it. The whole body is placed in a sterile plastic bag with 300 mL of sterile 0.1% Bacto peptone solution. A mechanical shaker is used to shake the body in peptone solution for about 1 minute. Collect 100 mL torso washes for bacterial analysis. The feces are removed aseptically and placed in a sterile plastic bag with 10 mL of 0.1% Bacto peptone solution. Mix each bag containing fecal matter at high speed with Stomacher for 2 minutes in solution.

5) Bacteria Count

The selected bacteria in the whole torso wash and excreta contents are counted. Lactobacillus bacillus and Campylobacter are calculated according to the method described by Hinton et al. E. coli was counted at 3M, Petrifilm, and C. perfringens were supplemented with TSC selection supplement B and egg yellows at perfringens Agar Base for 48 hours at 35 ° C in Coy Controlled Environment Chamber.

6) Statistical Analysis

The obtained data were analyzed at random using GLM procedures of SAS. Compare treatment methods using SAS 'PDIFF method when protecting against significant (P <0.05) treatment effects. Significant differences between treatment methods are defined by the least square mean ± pooled SEM reported using F-statistics. The% death and leg abnormality weight data is converted to arcsine before analysis and the SEM approximation is obtained from the actual weight percentage.

7) microbial activity

Bacterial colony progression of poultry trunk washes and excreta contents at 56 days showed no difference in Campylobacter and E. coli compared to poultry fed 0.1% or 0.2% by weight of plant probiotic supplements compared to the reference group. (p> 0.05).

Jin et al. Reported that the number of E. coli strains fed lactosan bacillus strains was lower than that of the reference group on days 10, 20, and 30, but no difference was found on day 40. In addition, the current study showed 9.28% (p <0.04) higher than the reference number of lactobacillus in the 56-day excretion content of poultry fed 0.20% live bacteria and 8.30% by weight compared to broilers fed 0.10% by weight live bacteria.

There was no difference in the number of colonies of lactobacillus in the feces of poultry fed the reference feed and 0.10% by weight of plant probiotic feed (p> 0.05). There was no difference in E. coli found in feces between poultry fed with 0.10% and 0.20% by weight of plant probiotics and poultry fed with reference feed.

As described above, the present invention also examined the effects of plant probiotics containing two lactic acid bacterium strains (L. salivarius and L. plantarum) in the intestine and intestine of poultry such as chicken on the intestinal bacteria and pathogenic microorganisms.

For this purpose, the diet was tested with a mixture of 0.10 wt% or 0.2 wt% of vegetable probiotics without reference to general poultry feed (reference feed containing insecticides and antibiotics), insecticides and antibiotics.

Investigations of the experiments showed that the poultry fed the diet supplemented with 0.1% or 0.2% by weight of plant live bacteria on days 1-42 showed no difference compared to the reference poultry (p> 0.05).

In addition, feed intake and feed-to-fat ratios for days 22-42 and 1-42 were lower (p> 0.001) in poultry fed with 0.10% or 0.20% by weight of plant live bacteria.

In addition, the number of colonies of Campylobacter jejun and Escherichia coli from 56-day-old poultry body washes and feces supplemented with 0.10% or 0.20% by weight of plant live bacteria was not significantly different (p> 0.05). Appeared.

Lactobacillus bacillus from 56 days old poultry dung fed feed supplemented with 0.20% by weight of plant live bacteria was higher (p> 0.04) than the group treated with reference feed and 0.10% by weight vegetable live bacteria.

The results showed that feed supplemented with plant probiotics containing L. plantarum and L. salivarius was similar in poultry growth and lower feed replacement rates than supplemented with basic insecticides and antibiotics. . This is believed to be due to the lactic acid bacteria bacillus strain contained in the feed of the present invention.

As described above, the feed composition for poultry containing the plant probiotics of the present invention showed a similar inhibitory effect on poultry feed, bacterial colonies (bacteria), and pathogenic microorganisms containing insecticidal insecticides and antibiotics, and the weight gain, meat quality, and storage of poultry. Even the delayed deterioration of meat has a synergistic effect.

In addition, it can be seen that the poultry survival can be maximized by excellent effects such as gastrointestinal protection of poultry, removal of intestinal inflammation and cell regeneration.

Although the present invention having been described above has been described with reference to a limited number of embodiments, it is to be understood that the present invention is not limited thereto and that various changes and modifications may be made without departing from the spirit and scope of the present invention by those skilled in the art. Various modifications and variations are possible within the scope of the appended claims.

Claims (2)

It contains 48 to 79.99% by weight of general poultry feed, 20 to 50% by weight of omega-3 fatty acids and 0,01 to 2.0% by weight of plant probiotics. Feed composition for poultry.
The method of claim 1,
The plant live bacteria is a feed composition for poultry containing plant live bacteria and omega-3 fatty acids, characterized in that the strain L. plantarum and L. salivarius obtained by fermentation of vegetables, fruits.

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