KR20130142697A - Method for manufacturing edible protaetia brevitarsis powder - Google Patents

Abstract

The present invention relates to a method for manufacturing edible protaetia brevitarsis larva powder and, more specifically, to a method for manufacturing edible protaetia brevitarsis larva powder including sterilizing, freeze-drying, and crushing processes for protaetia brevitarsis larvae. If the method for manufacturing edible protaetia brevitarsis larva powder is applied to protaetia brevitarsis for food products, the method for manufacturing edible protaetia brevitarsis larva powder can be effectively used to produce food which is hygienic and popular with customers. [Reference numerals] (AA) Powder image;(BB) Sawdust sterilization;(CC) Days of fasting

Description

Method for manufacturing edible white spotted flower larvae powder {Method for manufacturing edible Protaetia brevitarsis powder}

The present invention relates to a method for preparing edible white spotted flower larvae powder, and more particularly, to a method for producing edible white spotted flower larvae powder including sterilizing and lyophilizing and pulverizing the white spotted flower larvae. It is about.

After enacting the Insect Industry Promotion Act in 2010, it is necessary to expand the scope of the insect industry, but until now, many researches have been conducted on pollen mediation, learning, emotions, nemesis, and environmental purification. , Research on biomimetics and biotechnology is still inadequate. So far, only insects registered as food are grasshoppers and silkworms, so insects other than grasshoppers and silkworms are not recognized as food ingredients and cannot be sold for food. Should be done. As insects for which food registration should be prioritized, it is currently used as a folk remedy, and it is advantageous to target insects in which most farming farmers are established.

White-spotted flower chafer, Protaetia brevitarsis seulensis ) is an insect belonging to the coleoptera of the coleoptera, distributed in Korea, Japan, Taiwan, China, and Europe. It appears from early July, and the highest frequency of emergence is early August, and some adults overwinter. Overwinters as a third-larva larva. Eggs, larvae, chrysalis and adult insects are completely metamorphic through the age of the breeding farming farms, which are now mass-produced, is also called "slugs" of larvae belonging to the coleoptera. The white spotted larvae of the larvae in the agreement agrees with, “Menstrual irregularity, decreased vision, cataracts, gold swelling, postpartum wind, malignant boils, stomatitis, including liver disease, liver cancer, cirrhosis, hepatitis, and the elimination of accumulated fatigue. It is effective in treating adult diseases such as tetanus and stroke. ” Therefore, recently, research for functional analysis on white spotted flower ignorance has been actively conducted.

However, since the foodization of insects has problems such as external aversion and harmful bacteria, odor, etc., a new processing method is required to solve such problems.

Therefore, the present inventors studied a method for preparing edible white spotted radish larvae powder, and after fasting the white spotted radish larvae fed with oak fermented sawdust removed bacteria, sterilized by high temperature and high pressure sterilization and then lyophilized to dry powder As a result of the production of microorganisms were not present, and the color and smell of the powder was confirmed to complete the present invention.

Accordingly, it is an object of the present invention to provide a method for preparing edible white spotted flower larvae powder.

In order to achieve the object of the present invention, the present invention comprises the steps of breeding the fermented sawdust to white spotted larva larvae; (b) fasting the larva of step (a) for 1 to 5 days; (c) sterilizing the fasted larvae of step (b) at high temperature and high pressure; (d) lyophilizing the high temperature, high pressure sterilized larva of step (c); And (e) pulverizing the dried larva of step (d) to produce a powder.

Hereinafter, the present invention will be described in detail.

The manufacturing method of the present invention

(a) feeding the fermented sawdust to the white spotted radish larvae to breed;

(b) fasting the larva of step (a) for 1 to 5 days;

(c) sterilizing the fasting larvae of step (b) at high temperature and high pressure;

(d) lyophilizing the high temperature, high pressure sterilized larva of step (c); And

(e) pulverizing the dried larva of step (d), characterized in that it comprises the step of preparing a powder.

The manufacturing method will be described step by step as follows.

In step (a), the fermented sawdust is fed to the white spotted larvae to breed.

The white spotted radish of the present invention is an insect belonging to the coleoptera flowering radish family, but any larvae can be used. Preferably, 3 larvae can be used, and the fermented sawdust fed to the white spotted radish is a feed of white spotted radish. Any plant can be used as long as it can be used, but preferably, oak can be used. In addition, the fermented sawdust may be used by sterilization for 5 to 10 minutes with high temperature steam. In addition, the fermented sawdust feed sterilized for a certain period of time can reduce the intestinal microorganisms, can reduce the odor peculiar to insects. The feeding period is not particularly limited, but may preferably be 3 to 5 days. Feeding less than 3 days may reduce the intestinal microbial reduction effect, and feeding more than 5 days may be insufficient to increase the increase accordingly. In addition, the breeding environment may be a known insect breeding environment, and if the white spotted radish is a viable condition is not particularly limited and preferably breeding temperature can be maintained at 23 to 27 ℃.

In one embodiment of the present invention was steamed oak fermented sawdust after steam sterilization process confirmed that the presence of microorganisms were confirmed that the microorganisms are completely removed even 5 minutes.

In another embodiment of the present invention, the white spotted flower radish used three larvae purchased from the Smurf Insect Country Farming Corporation (Namyangju), and the breeding temperature was maintained at 25 ℃.

In step (b), the larva of step (a) is fasted for 1 to 5 days.

Through the fasting process, it is possible to remove the intestinal foreign substances by inducing bowel movement of the white spotted flower larvae. The fasting period is preferably fasting for 1 to 5 days, more preferably 3 to 5 days can be fasting. If the fasting period is less than one day, the intestinal foreign matter may not be sufficiently removed, and if it is more than five days, the larvae may die or the nutrition may deteriorate.

In one embodiment of the present invention induction of bowel hungry for 1 to 5 days without feeding water or food to the white spotted larva larvae, at this time the stool is missing and the white spotted blotch was used as a net (See Example 3).

In step (c), the fasting larvae of step (b) are autoclaved at high temperature.

 The high temperature and high pressure sterilization is a sterilization method for killing microorganisms by irreversible coagulation and denaturation of enzymes and tissue proteins with heated saturated steam, and temperature and pressure ranges may be used under conditions for general microorganism sterilization. High temperature and high pressure sterilization at 115-121 ° C., 0.9-1.3 kgf / cm 3 or more, for minutes.

In one embodiment of the present invention, the larvae were starved for 3 days and then sterilized by performing high-temperature sterilization at 115 ° C. and 0.9 kgf / cm 3 for 5 minutes, and the powder prepared by freeze-drying did not contain any microorganisms. Good results could be confirmed (see Example 4).

In step (d), the high-temperature, high-pressure sterilized larva of step (c) is lyophilized.

The lyophilization is a method of freezing a sample containing an aqueous solution or water and subliming the water under reduced pressure below the vapor pressure of the temperature to dry the water. The temperature and time range may be used for general lyophilization, preferably high temperature. The autoclaved larvae may be frozen in an ultra-low temperature freezer at -65 to -85 ° C. for 24 to 36 hours and then dried in a freeze dryer for about 48 to 60 hours.

In one embodiment of the present invention, larvae were randomly selected for 40 animals every 5 days for 24 hours and then frozen in an ultra-low temperature (-80 ℃) freezer for 24 hours or more and dried in a freeze dryer for about 48-60 hours (see Example 3). .

In step (e), the dried larvae of step (d) are ground to prepare a powder.

The pulverization process may use a pulverization process using a general pulverizer, preferably a method of making a fine powder powder by putting the dried larvae in the original shape into a clean crusher.

In one embodiment of the present invention, the larvae dried for about 48-60 hours to prepare a powder using a general crusher (see Example 3).

According to the present production method, it is possible to effectively produce odorless sterilized white spotted powder radish powder that can be applied to food.

In one embodiment of the present invention, the result of performing a sensory evaluation to evaluate the color and aroma of the white spotted flower radish powder, the sample starved for 3 days after feeding the sterilized oak fermented sawdust in both evaluation of the brightness and smell of color is the best I could be evaluated.

The present invention provides a method for producing an edible white spotted flower larva powder comprising the step of lyophilization and pulverization after fasting white spotted larva larvae. The method of preparing the edible white spotted radish larvae powder of the present invention is effective in producing foods that are more hygienic and higher in consumer preference when applied to white spotted radish for foodization.

1 is a graph showing the results of cytotoxicity measurement of white spotted radish larvae powder by food (Cell viability: Raw 264.7 cell growth rate, oak fermented sawdust: suspension of white spotted radish with oak fermented sawdust fermented with microbial culture) Treated group, fermented herb: group treated with suspension of white spotted radish fermented with fermented herb fermented with pig flour, fermented rice straw: group treated with suspension of white spotted radish stewed with natural fermented rice straw ).
Figure 2 is a cytotoxicity assay of white spotted radish powder according to the growth period (Larva: cytotoxicity in the treatment group of 3 larvae, Pre-pupa: cytotoxicity in the treatment group of larva just before soluble, Pupa: in the control group of pupa Cytotoxicity).
Figure 3 is a photograph of microbiological test results for setting oak fermented sawdust sterilization conditions (number in the picture is the same as Sample No. in Table 1).
Figure 4 is the variance (A), weight (B) and length (C) by date after starving white spotted larvae (Weight per larvae: weight of white spotted radish larvae fasted, days: white spotted blouse Starved days).
5 is an image of chapters by date after fasting white spotted larva larvae.
Figure 6 is the number of surviving heads by density in the same area (12 × 13 × 5 cm ³ ) when starving white spotted larvae.
7 is a white spotted larva larva freeze-dried powder color and odor sensory evaluation (n = 20) according to the number of days starved.
Figure 8 is white spotted flower solid powder sterilization conditions.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

≪ Example 1 >

White spotted flower breeding

White spotted radish was used as 3rd larvae purchased from Smurf Insect Country Farming Corporation (Namyangju), and the breeding temperature was maintained at 25 ℃. The breeding environment of white spotted flower larvae is about 40 cm (width) × 25 cm (length) × 15 cm (height) of transparent insect breeding container. After filling the oak fermented sawdust containing 20 larvae were reared.

<Example 2>

Cytotoxicity Measurement of White Spotted Flower Larva Powder

The experiment was carried out by dividing into three groups: fermented straw straw, oak fermented sawdust, and fermented herb. Breeding method was the same as in Example 1.

<2-1> star food White spotted flower  Preparation of suspension of larvae powder

White spotted larva larvae fed with fermented rice straw, white spotted larva larvae fed with oak fermented sawdust, white spotted larva larvae fed fermented herb for 3 days After freezing for 24 hours, the freeze dryer was dried until the moisture was completely removed. The dried powder was crushed with a grinder to obtain powder, and then put into DW (sterile water) and DMSO, respectively, and then pulverized twice with an ultrasonic mill (230 Jule) for 10 seconds. The pulverized powder was centrifuged at 4,500 rpm for 10 minutes and the supernatant was recovered and filtered through a 0.25 μm syringe filter.

<2-2> star food White spotted flower  Cytotoxicity Measurement of Suspension of Larva Powder

To determine the toxic effect on Raw 264.7, the macrophage of white spotted flower radish, cells were treated with suspension of white spotted radish and treated with MTS {3- (4,5-dimethylthiazol-2-yl) -5 after 24 hours. (3-carboxymethonyphenol) -2- (4-sulfophenyl) -2H-tetrazolium} reagent was added and incubated for 4 hours at 36 ° C. After incubation, the absorbance at 450 nm was measured using a microplate reader (Beckman, USA).

As a result, a mild toxicity was detected in the suspension of larvae powder fed fermented straw and sterile water, and there was no cytotoxicity when oak fermented sawdust and fermented grass were fed. It was judged that ignorance was suitable for foodization (see Fig. 1).

Next, as a result of confirming the cytotoxicity of white spotted radish according to the developmental stage, all treatments tested using DW (sterile water) and DMSO as solvents in all treatments of the 3rd larvae, larvae and pupae, which were the largest growth stages, were cells There was no toxicity, and especially in the treatment group tested using DW (sterile water) as a solvent, it was confirmed that the growth of cells was promoted. Therefore, the white spotted radish at all times used in the experiment is not a problem to use as food, but the white spotted mussel just before yonghua or chrysalis is difficult to collect and the selling price is about 1.5 times higher than the larvae. Since it is expensive, there is no cytotoxicity, it is reported as a medicinal book in ancient books, and it was determined that the third-larva larvae are cheap and easy to obtain (see FIG. 2).

< Example  3>

Measurement of larvae and larvae powder during fasting period

<3-1> according to sterilization conditions In feed  Residual Microbial Measurement

Bowel movement was induced to remove foreign substances in the gut of white spotted flower larvae. They did not feed the water or feed for 1 to 5 days starving to induce bowel movement. The net size was 50 mm x 50 mm. Each individual is 5 × 2.5 × 5 cm ³ per horse The bowel movement was induced for 5 days in the space of the size, and the experiment was conducted in three repetitions of 30 animals. Every 24 hours, the weight and length of each individual were measured and the bowel movement was checked. In order to measure the length of the larvae, it was left in ice for about 15 to 20 minutes before measuring the length.

On the other hand, oak fermented sawdust, which is used as food for white spotted radish, was fermented by mixing microorganisms. Therefore, it is thought that it contains many kinds of microorganisms. After the presence of microorganisms was confirmed.

As a result, it was confirmed that the microorganisms were completely removed when steaming for more than 5 minutes as shown in Table 1. Therefore, it was judged that feeding the fermented oak fermented sawdust sterilized for 3 days or more before freeze-drying would be good for reducing the number of microorganisms in the white spotted radish body (see FIG. 3). The numbers shown in FIG. 3 are the same as the Sample No. in Table 1.

<3-2> Larvae of the Fasting Period Defecation  Measure

As a result of checking the bowel movement at intervals of 24 hours for 5 days, a large amount of feces was excreted on the first day after starvation, and the bowel volume from the second day was confirmed to be a relatively small amount (see FIG. 4A). At this time, as a result of checking how much influence on the weight or length of each individual, it was confirmed that the weight and length decrease in proportion to the starving date (see FIGS. 4B and C).

Result of measuring microorganisms in oak fermented sawdust after sterilization Sample
No. Steaming time (minutes) microbe
(cfu / g) One × 7.5 × 10 ⁴ 2 5 - 3 10 - 4 20 - 5 30 -

<3-3> Fasting Days  Observation of larvae and larvae behavior

To determine how much intestinal material was removed through bowel movements, the intestines of starved larvae were dissected every 24 hours for 5 days. If left in ice for about 15 to 20 minutes before dissection, the body is stretched in a stunned state. The dissection was carried out while the larvae were soaked in 1 × PBS. After the intestines were extracted, the larvae were immersed in 1 × PBS and dried. As a result, it was confirmed that the lieutenant was emptied from the third day after starving (see Fig. 5). For reference, we checked for carnibalism during hunger. The reason is that if there is carnivalism, it is difficult to starve by putting white spotted flowers in a container. As a result of starving for 5 days in 20 ^~ 100 dogs in a certain area (12 × 13 × 5 cm ³ ), 100 dogs were added, and most of them survived for 5 days in high density. It was judged that it was possible to starve in a container (see Fig. 6).

<3-4> Fasting Days  Sensory Evaluation of Larva Powder

Using the powder prepared for food production of white spotted radish larvae, sensory evaluation using human smell and sight was carried out to evaluate color and aroma. The subjects of sensory evaluation were randomly selected 20 men and women between 20s and 40s. The odors of larvae are presumed to be mainly caused by the substances present in food and intestine. Starved for 5 days. Forty-five larvae were randomly selected for 5 days every 24 hours, frozen in an ultra-low temperature (-80 ℃) freezer for more than 24 hours, and then dried for about 48 to 60 hours in a freeze dryer. Sensory evaluation was performed to evaluate color and aroma.

As a result, in the two evaluations of color brightness and smell, the samples starved for 3 days after sterile oak fermented sawdust received the best rating. Based on the above results, it was judged that feeding the sterilized oak fermented sawdust and then starving for 3 days was most suitable for pretreatment conditions (see FIG. 7).

< Example  4>

Residual microbial measurement according to sterile powder sterilization conditions

All materials used as food should not be harmful to the human body, so high temperature and high pressure sterilization was performed to remove the bacteria larvae. In order to minimize the destruction of the larvae effective substances under high temperature and high pressure, high temperature and high pressure sterilization was performed under several conditions. After high temperature and high pressure sterilization at 115 ° C, 0.9 kgf / cm 3 and 121 ° C, 1.3 kgf / cm 3 for 5 minutes, the presence of bacteria and fungi is shown in Table 2. LB (Luria-Bertani) solid medium was used as a medium for bacteria identification, and PDA (Potato Dextrose Agar) solid medium was used as a medium for fungal identification. After dissolving the prepared white spotted powdered radish powder in sterile water, each sample was smeared on a solid medium and bacteria were incubated at 37 ° C. for 24 hours, and the fungus was incubated at 25 ° C. for 48 hours to confirm the presence of microorganisms.

As a result, it was confirmed that the bacteria and fungi were removed when the 115 ° C. and 0.9 kgf / cm 3 pressure were treated for 5 minutes (see FIG. 8). The numerals shown in FIG. 8 are the same as the Sample No. in Table 2.

Therefore, it was confirmed that the use of the third-larvae larvae fed with oak fermented sawdust as a pretreatment condition for foodization of white spotted larva larvae was not cytotoxic and economical. The larvae fed with oak fermented sawdust, which had been steamed for 5 minutes with steam under powder pretreatment conditions for 3 days, were starved for 3 days, then sterilized by high temperature and high pressure sterilization at 115 ° C. and 0.9 kgf / cm 3 for 5 minutes, and then lyophilized. It was confirmed that the prepared powder did not have microorganisms and the color and smell of the powder were good.

White spotted flower  High temperature and high pressure Sterilized  Of bacteria and fungi Presence Sample
No. High Temperature High Pressure Sterilization Germ
(cfu / g) Fungus
(cfu / g) One × 2.8 × 10 ⁵ 2.3 × 10 ⁵ 2 115 ° C, 0.9 kgf / cm ³ , 5min - - 3 115 ° C, 0.9 kgf / cm ³ , 10min - - 4 121 ° C, 1.3 kgf / cm ³ , 5min - - 5 121 ° C, 1.3 kgf / cm ³ , 10min - -

As described above, the present invention provides a method for preparing edible white spotted flower larvae powder. The manufacturing method of the edible white spotted radish larvae powder of the present invention can be effectively used to prepare foods with higher hygiene and consumer preference when applied to the white spotted radish for foodization, and thus has high industrial applicability.

Claims (5)

(a) feeding the fermented sawdust to the white spotted radish larvae to breed;
(b) fasting the larva of step (a) for 1 to 5 days;
(c) sterilizing the fasting larvae of step (b) at high temperature and high pressure;
(d) lyophilizing the high temperature, high pressure sterilized larva of step (c); And
(e) a method of producing white spotted flower radish powder, comprising the step of preparing a powder by pulverizing the dried larva of step (d).
The method of claim 1, wherein the fermented sawdust is sterilized oak fermented sawdust manufacturing method of white spotted flower radish powder.
The method according to claim 1, wherein the breeding is carried out for 3 to 5 days, characterized in that the white spotted flower radish powder.
The method according to claim 1, wherein the freeze-drying is a method for producing white spotted flower radish powder, characterized in that performed in a freeze dryer for 48 to 60 hours.
The method according to claim 1, wherein the high temperature and high pressure sterilization is carried out at 115 to 121 ° C for 0.9 to 1.3 kgf / cm 3 for 5 to 15 minutes.

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