KR20130131875A - Manufacturing method of functional fermentation products using cheju scoria and functional plants and culturing method - Google Patents

Abstract

The present invention relates to methods of: preparing a Jeju scoria-fermented culture medium, which is a composition for nutrient culture and has functionality by fermenting Jeju scoria, which contains various materials, such as vanadium, selenium, zinc, and manganese and is abundantly scattered all over the Jeju Island, with microorganisms; culturing a plant by using the prepared Jeju scoria-fermented culture medium which is a composition for nutrient culture; and using the cultured functional plant. [Reference numerals] (S10) Preparing Jeju scoria-fermented culture medium;(S20) Soaking;(S30) Culturing plant by using culture medium;(S40) Obtaining plant resources

Description

Jeju pine fermentation broth extracted by microbial fermentation and cultivated functional plants using the same and cultivation method thereof {Manufacturing Method of Functional Fermentation Products using CHEJU Scoria and Functional Plants and Culturing Method}

The present invention contains various substances such as vanadium, selenium, zinc, manganese, and the like. The present invention relates to a method for preparing pine mushroom fermentation broth, cultivating the plant with the Jeju pine fermentation broth prepared as a nutrient cultivation composition, and using the grown functional plants.

Jeju Scoria, scattered throughout Jeju Island, is known to contain various substances.

The technology to use Jeju Songju (Scoria) containing these various substances is provided as below, and it is still developing technology using Jeju Songju.

(KR) In the method for preparing a zeolite material using Jeju clusters of Patent No. 301790, there is provided a method for producing a zeolite substance by hydrothermal synthesis of a Jeju cluster containing aluminum oxide using a sodium hydroxide solution which is a basic substance. have.

In addition, the academic paper published in 1994 (published by the Korean Society for Bio-environmental Control) provides a description of the changes of nutrient components in cherry tomato nutrient cultivation using Jeju pine. In the above-described technology, the Jeju pine is simply immersed in a plastic seedling box and then cultivated cherry tomatoes.

The present applicant intends to provide a technology for using nutrient solution such as vanadium, selenium, zinc, manganese, which is contained in Jeju cultivation, and to grow functional plants using the same.

Diabetes mellitus can be classified into type 1 diabetes and type 2 diabetes before birth.

The type 1 diabetes (insulin-dependent diabetes mellitus) is caused by an abnormal insulin secretion in the pancreas, which is caused by the autoimmune reaction of the insulin-producing beta cells of the insulin-producing pancreas, resulting in insulin secretion . In contrast, type 2 diabetes mellitus is normal in insulin secretion in the beta cells, but it is highly insulin resistance in the peripheral tissues, resulting in an abnormal glucose metabolism, called adult diabetes mellitus, about 90% of all diabetics.

When insulin resistance increases due to obesity, insulin secretion increases continuously and causes complications. Finally, insulin-producing pancreatic cells lose their function.

In the course of progression to type 2 diabetes, the stage of pre-diabetes is preceded by the pre-diabetes (Predaibetes) is the term between normal and diabetes, impaired glucose tolerance and fasting glucose is a term.

The impaired glucose tolerance refers to when the plasma glucose concentration is 141 to 199 mg / dl after 2 hours in the oral glucose tolerance test, and the fasting glucose level indicates the fasting plasma glucose concentration is 111 to 125 mg / dl.

Prediabetes is a pre-diabetes stage that is both a risk factor for type 2 diabetes and a risk factor for cardiovascular disease, but its prevalence increases. Exercise therapy and diet is recommended instead of special treatment due to the risk of induction.

Therefore, it is necessary to develop a material that can prevent or prevent treatment before it reaches diabetes.

One of the diets was the intake of vanadium-containing foods. About 30 years ago, Van Gelder's Yoram Shechter of the Wiseman Institute in Israel found that vanadium, a trace metal element, is similar to the metabolic effect of insulin And found that administration of vanadium to diabetes can cure a variety of metabolic defects due to diabetes. In addition, several collaborators in Japan have found that vanadium, which is a kind of minerals, has the effect of improving diabetes, and that vanadium improves diabetes.

In addition, as a study to utilize vanadium,

VANADIUM ION-CONTAINING SUGAR, LIPID AND / OR NITROGEN METABOLIC DISEASE-IMPROVING AGENT ((JP) Patent Publication No. 2007-031282) is an agent for improving or treating diseases related to glucose metabolism or diabetes metabolism or nitrogen metabolism. The present invention provides a sugar, nitrogen, and / or lipid metabolism improving agent, wherein the beverage contains at least 80 µg / L of vanadium ions (V), and the content of vanadium ions (V) is preferred. Preferably it is 100-300 microgram / L, More preferably, it is 200-250 microgram / L, As one Embodiment of this invention, the said drink is possible to utilize form, such as mineral water, green tea, black tea, juice, or lactic acid bacteria drink. Technology research,

VANADIUM ION-CONTAINING AGENT FOR AMELIORATING HYPERTENSION, HYPERLIPEMIA AND NEUTRAL-FAT LIPEMIA AND BLOOD SUGAR-LOWERING AGENT FOR HYPERLIPEMIA AND NEUTRAL-FAT LIPEMIA PATIENT ((JP) Patent Publication Number: 2006-232679) A technology that verifies the effects of drinking vanadium ion water over a period of time and provides the use of vanadium ionized water based on the results of these treatments to provide confidence in adult disease. The concentration of vanadium ion (V) at least 80 µg / L In the form of the aqueous solution containing, characterized in that it provides a hypoglycemic agent used for the prevention or treatment of diabetes, such as a hypertension improving agent used for the prevention or treatment of hypertension in a mammal,

METHOD FOR PRODUCING VANADIUM-CONTAINING MYCELIUM OF AGARICUS AND FOOD HAVING AGARICUS FUNCTION Provided is a functional food containing vanadium and a production method for producing the functional food efficiently and efficiently. A species culture solution of Agaricus mycelium mycelium in a culture medium containing a water-soluble vanadium compound together with culture components such as a carbon source, a nitrogen source, a mineral source, and water. Agaricus mushroom mycelium is inoculated and grown to obtain Agaricus mushroom mycelium containing vanadium.The Agaricus mushroom mycelium is separated from the liquid using a separator such as a centrifugal separator, dried with a dryer, and then pulverized with a pulverizer. A device characterized by obtaining a functional food composed of a powder And

VANADIUM-CONTAINING RADISH SPROUT AND METHOD FOR CULTURING THE SAME ((JP) Patent Publication No. 2006-166833) contains vanadium which is said to be an effective element for the prevention of diabetes and contains vanadium as a food that can be easily eaten on a daily basis. By providing young cotyledon radish, young cotyledon radish grown by feeding seeds of germinated young cotyledon radish into hydroponic culture water tank and supplying liquid fertilizer of predetermined temperature including soluble kali, phosphorus, nitrogen, etc. It is a cultivation method, It adds soluble compound of vanadium to the said liquid fertilizer, and grows, It is preferable to make the vanadium concentration in a liquid fertilizer into 0.05-10 mg / L in this young cotyledon cultivation method. The vanadium content of young cotyledon radish produced by the method is preferably about 10 to 100 ppm. .

As described above, research on the production of materials and products using vanadium has been conducted, but systematic research is still insignificant, and the present technology also reduces the incidence through the prevention of diseases including diabetes, and at the same time, functional materials that can serve as therapeutic aids. (Or raw material) is a technology relating to production.

(KR) Patent registration No. 301790 (Registered on June 28, 2001) (JP) Patent Publication No. 2007-031282 (JP) Patent Publication No. 2006-232679 (JP) Patent Publication No. 2006-180716 (JP) Patent Publication No. 2006-166833

Changes of Nutrient Components in Cherry Tomato Nutrient Cultivation Using Jeju Cluster (Preparation Year: 1994. Publisher: Korean Society for Bio-Environment Control)

The present invention is to provide a functional material using Jeju clusters containing various components such as vanadium, selenium, zinc, vanadium (vanadium) that is contained in the Jeju clusters through microbial fermentation ), Selenium (selenium), zinc and the like, and to provide the fermentation broth in Jeju pine available for cultivation of nutrients,

The present invention also provides a nutrient cultivation method using the Jeju cultivation fermentation broth, which is intended to establish the nutrient solution cultivation conditions of various plants using the fermentation broth,

In addition, to provide a functional plant cultured nutrient solution using the Jeju pine fermentation broth.

The Jeju pine fermentation broth according to the present invention for achieving the above object is obtained by extracting useful components of Jeju clusters by fermenting the Jeju clusters using microorganisms, the microorganisms used in the fermentation are Bacillus sp. And Streptomyces sp. It is used individually or mixed, respectively.

The nutrient solution cultivation method for cultivating functional plants using the Jeju pine fermentation broth extracted by the fermentation of microorganisms in this way, the preparation step (S10) to ferment the Jeju pine by the microorganisms to prepare the Jeju pine fermentation broth; A soaking step (S20) of sowing plant seeds in Jeju fermentation broth prepared in step S10; The plant seed called in step (S20) is characterized in that it comprises a nutrient solution cultivation step (S30) of cultivating using the fermentation broth Jeju jeju prepared in the step (S10).

The microorganism used in the Jeju pine nutrient solution preparation step (S10) is Bacillus sp . With Streptomyces sp . It is used individually or mixed, respectively.

Plant seed in the soaking step (S20) is soaked in 3-5 hours soaked in Jeju fermentation broth prepared in the step (S10).

Disease prevention and treatment for the treatment of each functional plant containing vanadium, selenium, zinc or manganese as an active ingredient in the functional plant grown by the nutrient solution cultivation method of the functional plant using the Jeju pine fermentation broth extracted by the microorganism fermentation I can do it with material.

Jeju pine fermentation broth is extracted by the microorganism fermentation of the present invention and used as a nutrient solution used when nutrient solution is excised to extract the components such as vanadium, selenium, zinc, manganese contained in the Jeju pine by fermenting the Jeju cluster with microorganisms, By using this to produce a nutrient solution for nutrient solution cultivation can be used to provide a fermentation broth (nutrient solution) Jeju containing vanadium, selenium, zinc and the like to grow functional plants.

In addition, the functional plant obtained by the nutrient solution cultivation method using the Jeju pine fermentation broth extracted by the microorganism fermentation as described above can promote the public health.

In addition, the functional plants grown by the nutrient solution cultivation method using the Jeju pine fermentation broth extracted by microbial fermentation as described above contains a large amount of vanadium, selenium, zinc, manganese, etc. for the prevention and treatment of various diseases such as diabetes. It can be used as a functional material.

That is, the present invention, it was confirmed that vanadium having diabetes prevention and therapeutic efficacy accumulated in the nutrient solution culture using the Jeju pine fermentation broth, and also selenium, zinc, manganese and the like.

1 is a step configuration of obtaining a fermentation broth fermentation broth extracted by the microbial fermentation showing an embodiment of the present invention and cultivating the plant using the same.
Figure 2 is a schematic diagram showing the cultivation of nutrient solution, which is a fermentation broth of Jeju, obtained by fermenting Jeju clusters with microorganisms alone or by putting Jeju clusters or jade.
Figure 3 is a photograph of sprout rapeseed nutrient-cultivated short-term crops (plants) with the Jeju pine fermentation broth obtained by fermenting Jeju matsutake with microorganisms.
Figure 4 is a photograph of the sprout barley cultivated short-term crops (plants) into the Jeju pine fermentation broth obtained by fermenting Jeju matsutake with microorganisms.
Figure 5 is a photograph of Chinese cabbage sprouts cultivated short-term crops (plants) into the Jeju pine fermentation broth obtained by fermenting the Jeju matsutake microorganisms.
Figure 6 is a graph examining the effects of stability and external factors from HepG2 hepatocytes (serum containing medium) of Jeju pine fermentation broth (FMJS).
Figure 7 is a graph examining the effect of stability and external factors from HepG2 hepatocytes (serum deficient medium) of Jeju pine fermentation broth (FMJS).
8 is a graph examining the cytoprotective effect of LJ skeletal muscle cells of Jeju pine fermentation broth (FMJS).
9 is a graph examining the protective effect of hepatocellular damage induced by oxidative stress of Jeju pine fermentation broth (FMJS).
10 is a graph examining the cytoprotective effect of Jeju pine fermentation broth (FMJS) on Acetaminophen-induced hepatotoxicity.
11 is a graph examining the effect of Jeju pine fermentation broth (FMJS) on glucose absorption in L6 skeletal muscle cells.
12 is a graph examining the effect of Jeju pine fermentation broth (FMJS) on lipid accumulation in hepatocytes (HepG2).
FIG. 13 is a graph illustrating the change of cells (nuclei) through LDT, MTT analysis and fluorescence staining in muscle cells (L6) of plants (a) cultured with Jeju pine fermentation broth (FMJS).
FIG. 14 is a graph illustrating the change of cells (nuclei) through LDT, MTT analysis and fluorescence staining in muscle cells (L6) of plants (d) cultured with Jeju pine fermentation broth (FMJS).
FIG. 15 is a graph illustrating the effects of nutrient-cultivated plants on Jeju glucose fermentation broth (FMJS) on glucose absorption in L6 skeletal muscle cells. FIG.

The terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms and the inventor may appropriately define the concept of the term in order to best describe its invention It should be construed as meaning and concept consistent with the technical idea of the present invention.

Therefore, the configuration described herein is only the most preferred embodiment of the present invention and does not represent all of the technical idea of the present invention, there may be various equivalents and modifications that can replace them at the time of the present application Should be understood.

The present invention obtains Jeju pine fermentation broth by fermenting Jeju pine (S Korea) containing many useful components such as vanadium (selenium), manganese, zinc, etc. with a microorganism, using the fermentation broth Vanadium by nutrient cultivation. A functional plant containing selenium, zinc, manganese, etc. is obtained, and this plant provides various functional materials as an active ingredient. In particular, plants containing vanadium will provide a functional material for the prevention and treatment of diabetes diseases.

The functional material includes food and medicine.

Hereinafter, the present invention will be described in detail.

Jeju cultivation fermentation broth containing vanadium, zinc, manganese and the like according to the present invention and the cultivation of plants using the same are prepared by the following method.

Referring to Figure 1,

1. Preparation S10 )

Jeju cultivation is used to prepare fermentation broth, which contains various useful ingredients such as vanadium, selenium, zinc, and manganese while helping plants germinate and grow.

Preparation step (S10) for producing the Jeju pine fermentation broth, microorganisms ( Bacillus sp. And Streptomyces sp . Fermentation of Jeju clusters using a single or a mixture of them) to extract various useful components such as vanadium, selenium and zinc contained in Jeju clusters to prepare fermentation broth used for cultivation of nutrient solution,

After the fermenter into a bunch of Jeju and the sugar and water microorganisms (Bacillus sp . And Streptomyces sp . Each of them may be used alone or mixed, in this study Bacillus sp. : Streptomyces sp. = 2: 8 mixed) and fermented at room temperature (30 ± 5 ℃) for 4-15 days.

Bacillus in the use of the microorganism sp . With Streptomyces sp . Can be used alone, or can be used even if mixed in a certain ratio.

Bacillus sp . When used alone, the vanadium content was measured as 2.5 mg / L, Bacillus sp . With Streptomyces sp . In the case of using the mixtures (2: 8), the vanadium content was measured to be up to 3.8 mg / L.

As a result of analyzing fermentation culture of Jeju pine fermented in fermenter, it contained (0.230 ~ 0.310) mg / L vanadium which can be used for cultivation from 3-4 days, and the vanadium content was (2.90 ~ 3.80) mg / L, after which there was little change in the content of vanadium content.

However, the number of fermentation days may vary depending on the fungus and bacterial condition.

2. The so called stage S20 )

Using the prepared Jeju pine fermentation broth (nutrient solution) is soaked by plant seeds for 3 to 5 hours.

The plant seeds are seeds in the case of seed propagation plants, stems in the case of stem propagation plants, roots in the case of root propagation plants, and are sources (mediations) for propagation of plants.

As described above, some of the plant seeds may be germinated when soaked in Jeju fermentation broth, and thus, in the case of plants in which plant seeds are soaked in the fermentation broth of Jeju pine, it is contained in the fermentation broth. The plant contains a large amount of useful components.

At this time, the pH of the Jeju pine fermentation broth used as the soaking liquid is preferably used to adjust to 6.4 ± 0.3.

3. Nutrient growing step ( S30 )

The soaked plant seeds were cultivated using Jeju pine fermentation broth (nutrient solution) to obtain plants containing vanadium, selenium, zinc, manganese and the like.

In the nutrient solution cultivation step (S30), it is preferable to use soaked plant seeds, but it is also possible to use soy plant seeds.

At this time, the Jeju cultivation fermentation broth may be sprayed with a nebulizer at regular intervals about two to twelve times a day to be cultivated (one spray amount is 4-11 ml / 100 cm 2).

In this case, the nutrient cultivation period takes about 4-9 days for short-term crops such as sprout vegetables, and the nutrient cultivation period takes 10 days or more for medium and longer crops.

For cultivation of short- and medium-term crops, Jeju pine fermentation broth can be used alone, and Jeju pine fermentation broth and other nutrient solutions can be used.

Figure 2 shows an embodiment of cultivating the nutrient solution using the Jeju pine fermentation broth of the present invention, it can be cultivated in a variety of ways, such as (a) to (c) of FIG.

That is, the plant seed (2) called in the nutrient cultivation container (1) containing the Jeju pine fermentation broth (3) as shown in Figure 2 (a) can be grown, and the Jeju pine fermentation broth (3) as shown in (b) ) And the plant seed (2) called nutrient solution cultivation container (1) containing Jeju clusters (4) can be cultivated, and as shown in (c), the Jeju pine fermentation broth (3) and jade or common stone (5) Plant seeds 2 soaked in the nutrient cultivation container 1 contained therein can be grown.

The plants used are (sprout) rapeseed, (sprout) buckwheat, (sprout) barley, multicolored sprout, (sprout) broccoli, (sprout) bok choy, stevia, red radish sprout, turnip sprout, (sprout) pea, (sprout) cabbage , (Sprout) sapling radish, (sprout) radish sprouts, buttercups, sprouts, stevia and the like can be used.

4. Plant obtaining step ( S40 )

As described above, in the case of plants containing vanadium and zinc contained in Jeju cultivation obtained by fermentation culture (nutrient solution) in Jeju pine mushroom, the product is formulated into various formulations including health beverage through juice, extraction, and enzyme treatment. It is also possible and can be taken directly without processing.

In addition, by analyzing the components of the cultivated plant nutrient solution plant containing a large amount of vanadium can be added to the functional material or health (functional) food for prevention and treatment for the purpose of preventing diabetes and improving diabetic disease, Plants containing a large amount of zinc can be added to functional materials or health foods for the prevention and treatment thereof for the purpose of preventing hair loss and taste deterioration.

When the plant of the present invention is used as a food additive, it can be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment).

The health beverage composition of the present invention may contain various flavors or natural carbohydrates, and the like as additional components, as in general beverages. Natural carbohydrates described above are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like.

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. As described above, the embodiments are provided only to easily understand the present invention, but the contents of the present invention are not limited to the embodiments.

One. Example  : Jeju cluster  Fermented with microorganisms Jeju pine  Fermentation Culture

After dividing the scoria by size, put 2 kg, 4 kg of sugar, 1 kg of sugar, and 20 L of water into the fermenter, respectively, and then put microorganisms ( Bacillus in this study). sp . Streptomyces sp . = 2: 8 mixed) and fermented at room temperature (30 ° C) for 6 days. But the microorganism is Bacillus sp . With Streptomyces sp . Each can be used alone, mixed two bacteria ( Bacillus sp . Streptomyces sp . = (1 ~ 9) :( 9 ~ 1)) can also be used. It is also possible to adjust and adjust the fermentation days.

In this way, the fermentation broth obtained by microbial fermentation is filtered to prepare fermentation broth in Jeju.

As a result of analyzing the main components of the Jeju pine fermentation broth prepared in the same manner as above, as shown in Table 1, 0.834 ppm of vanadium, 68ppm of zinc, 384ppm of manganese, 0.085ppm of selenium, etc. Was analyzed.

Contents of main components of Jeju pine fermentation broth (nutrient solution) (Unit: ㎎ / ℓ) division Mn Fe Co Ni Zn Pb CD Se V Jeju pine cultivation solution
Fermentation broth 384 118 - - 68 - - 0.085 0.834

2. Jeju cluster  Obtained by fermentation with microorganisms Jeju pine  Plant cultivation using fermentation broth

A nutrient preparation step (S10) of preparing a nutrient solution (Jeju pine fermentation broth) necessary for nutrient solution cultivation using the Jeju cluster; A soaking step (S20) of calling the plant seeds using the nutrient solution (Jeju-song fermentation broth) prepared in the step (S10); A nutrient cultivation step (S30) of cultivating the plant seeds finished in the step (S20) using the Jeju pine fermentation broth prepared in the nutrient preparation step (S10); Plants are cultivated as a plant acquisition step (S40) to obtain a plant obtained by cultivating the process of the step (S30) to obtain the quality by analyzing the main components derived from Jeju nutrient solution.

More detailed configuration is as follows.

Jeju cluster  using For nutrient cultivation  necessary Jeju pine  Fermentation broth ( Nutrient solution Ready)

As in the above example, Jeju clusters (scoria) were divided by size, and 2 kg, 4 kg of saccharides, and 1 kg of water, 20 L of 4-6 mesh, respectively, were placed in a fermenter, followed by fermentation at room temperature (30 ° C.) for 6 days. Let's do it. In this embodiment, the microorganisms were mixed ( Bacillus sp .: Streptomyces sp . = 2: 8), but in general, the microorganisms were Bacillus sp. With Streptomyces sp . Each can be used alone, mixed two bacteria ( Bacillus sp . Streptomyces sp . = (1 ~ 9) :( 9 ~ 1)) can also be used. It is also possible to adjust and adjust the fermentation days.

Jeju fermentation broth obtained by fermentation was vanadium content of 0.834 mg / l, zinc content of 68.0 mg / l, manganese content of 384 mg / l, selenium content of less than 0.1 mg / l, but 0.085 mg. / l was analyzed. This fermentation product is filtered to adjust the pH to 6.4 ± 0.3 to prepare fermentation broth (nutrient solution) in Jeju.

Jeju pine  Fermentation broth ( Nutrient solution Sowing plant seeds

The plants are soaked for 4 hours using the prepared Jeju pine fermentation broth (nutrient solution).

Fermentation of Seed Plant Seeds with Fermentation Culture Solution

The soaked plant seeds are cultivated using the Jeju pine fermentation broth (nutrient solution).

At this time, the Jeju cultivation fermentation broth (nutrient solution) to the cultivated plant is sprayed in an amount of 6ml / 100㎠ once a sprayer at regular intervals four times a day.

Cultivation period takes 3 ~ 9 days for short-term crops (sprout vegetables, etc.) and more than 10 days for mid- to long-term crops.

Jeju pine  Fermentation broth ( Nutrient solution ) Nutrient-grown  plant

Plants are harvested by cultivating 3 to 9 days for short-term crops and 10 days or longer for mid- to long-term crops.

Harvested plants are analyzed for the content of vanadium, zinc, selenium, etc. in order to ensure the quality of the plants after drying.

3. Jeju pine  Fermentation broth ( Nutrient solution )to Nutrient-grown  Analysis of Contents of Major Components of Plants

Plants cultivated with Jeju pine fermentation broth (nutrient solution) were dried and pretreated in the following manner and analyzed for vanadium content using ICP-AES to obtain the results shown in Table 2.

The pretreatment process takes about 0.2500 to 2.000 g of the sample in a Erlenmeyer flask or beaker, adds 10 mL of HNO ₃ solution, and heats / stirres. When the sample is completely decomposed, HNO ₃ is blown out and ₃ mL of HNO _{3 is} added to the Erlenmeyer flask again. After heating, add H ₂ O ₂ until no foam is generated (reaction with organic matter, CO _{2 is} generated), and when the reaction is completed, it becomes a transparent color, and is almost evaporated, cooled, and filled with distilled water to a certain volume (diluted) Do not exceed 50 times the drainage), ICP-AES to analyze the content of the main components such as vanadium.

As shown in Table 2 below, rapeseed of plant resources was analyzed to be 3.68ppm of vanadium, 91ppm of manganese, and 54ppm of zinc based on dry matters, and 2.04ppm of vanadium and 71.82ppm of manganese. , 76.26ppm of zinc and 2.65ppm of selenium were analyzed. In the case of bibly sprouts, 1.96ppm of vanadium, 48.36ppm of manganese, 28.36ppm of zinc and 2.01ppm of selenium were analyzed. In addition, buckwheat sprouts, barley barley and red radish sprouts accumulate vanadium.

Contents of Major Components of Plants Cultured with Fermentation broth (nutrient solution) in Jeju (unit: ㎎ / ㎏) division Cr Mn Fe Co Zn As Pb CD Se V Sprout oil - 91.23 77.34 - 54.38 - - - - 3.68 Sprout cabbage - 71.82 58.64 - 76.26 - - - 2.65 2.04 Bibimb sprout - 48.36 58.34 - 28.36 - - - 2.01 1.96 Sprout buckwheat - 28.45 48.68 - 29.24 - - - - 1.68 Sprout barley - 22.02 34.26 - 30.84 - - - - 1.86 Red radish - 18.8 28.2 - 26.34 - - - - 1.78

4. Jeju pine  Fermentation broth ( Nutrient solution Confirmation of efficacy

Safety and Cellular Protective Effects of Jeju Fermented Fermentation Broth from Cells (HepG2 Hepatocytes and L6 Skeletal Muscle Cells):

Fermented Media of Jeju Scoria (FMJS) was cultured in HepG2 cells, which are liver tissue cell lines, with or without serum, respectively, and Jeju Fermentation broth was contained in the culture medium (0-5%, v / v) and cultured for a certain period of time (48 hours) to measure the cytotoxicity LDH and cell survival indicator MTT activity.

Recognized cells on MTT (A in FIG. 6) and LDH (B in FIG. 6) cells when cultured for 48 hours by adding up to 5% of Jeju pine fermentation broth to a medium containing 10% serum (FBS) No toxicity was observed and no signs of apoptosis were observed from the cell fluorescence staining results (FIG. 6C).

 HepG2 cells were cultured for 48 hours in serum deficient cultures to induce nutrient deficient cell death. In this case, it was examined whether addition of Jeju pine fermentation broth inhibited nutrient deficient cell death (see FIG. 7).

When 48 hours of HepG2 cells were cultured in a serum-containing nutrient deficient medium, cell survival (MTT, A in FIG. 7) was decreased, and the content of LDH (C in FIG. 7) in the culture medium, a cytotoxic marker, was increased. Condensation of the cell nucleus (FIG. 7C), which is one of the indicators of trophic apoptosis, was observed.

However, the addition of the Jeju pine fermentation broth to the medium showed a protective activity of inhibiting such cell damage. These results show that Jeju pine fermentation broth does not cause toxicity to hepatocytes (HepG2) in normal culture, but has a cytoprotective effect that inhibits trophic cell damage.

L6 skeletal muscle cells reproduced whether the cytoprotective effect in hepatocytes was the same in skeletal muscle cells, which is an anti-diabetic target cell, and as a result, Jeju pine fermentation broth showed no-deficient cell damage in skeletal muscle cells. Inhibitory cytoprotective effect was observed (see FIG. 8).

As in hepatocytes, Jeju pine fermentation broth was found to protect the cytotoxic effect on skeletal muscle cells.

It was confirmed that Jeju pine fermentation broth had an effect of protecting liver cells from cell damage caused by excessive oxidative stress in addition to nutrient deficient cell damage.

To induce oxidative stress, hepatotoxicity was induced in HepG2 cells by treatment with excess oxygen (H ₂ O ₂ ), a reactive oxygen speies (ROS), and the Jeju pine fermentation broth (FMJS). . As a result, it was confirmed that cell damage caused by oxidative stress (LDH increase, MTT decrease, cell nuclear condensation, and fragmentation) was significantly suppressed by parallel treatment of Jeju pine fermentation broth (see FIG. 9).

This study was conducted to investigate whether the Jeju pine fermentation broth (FMJS) has a cytoprotective effect on cytotoxicity induced by drug in hepatocytes. To this end, hepG2 cells were treated with acetaminophen, which induces hepatotoxicity at high concentrations, and FMJS was concurrently treated (see FIG. 10). As a result, high concentration (20 mM) of acetaminophen treatment induced a wide range of cell damage, and when Jeju pine fermentation broth (FMJS) was treated in parallel, these indicators of cell damage were reduced.

As a result, the fermented media of Jeju Scoria (FMJS) prepared using Jeju cultivars was not able to observe the toxicity to cells by itself, but the cytoprotective effect against various cell damage factors. It was confirmed that it has. These results show that Jeju pine fermentation broth (FMJS) is used as a nutrient solution for various crop cultivation, and the crops cultivated using this nutrient solution proved the stability and cytoprotective effect at the cellular level to derive anti-diabetic effect. will be.

6. Jeju pine  Fermentation broth ( Fermented Media of Jeju Scoria  : FMJS ) On Glucose Absorption in Skeletal Muscle Cells

Insulin-independent diabetes mellitus is due to the inadequate uptake of glucose present in the blood by insulin target organs, including skeletal muscle, and consequently, the discovery of materials that increase glucose uptake in insulin target organs is an important part of antidiabetic research. wet.

In this experiment, the effect of Jeju pine fermentation broth (FMJS) on glucose uptake in L6 skeletal muscle cells was examined (see FIG. 11).

As a result, Jeju pine fermentation broth (FMJS) for cultivation of nutrient solution prepared using Jeju pine was found to have the effect of promoting glucose uptake by skeletal muscle cells alone without the help of insulin.

Especially at low concentrations (1,2%, v / v), the effect was greatest, but at higher concentrations, the effect tended to decrease again. These results suggest that Jeju pine fermentation broth (FMJS) has the effect of promoting glucose absorption in skeletal muscle in a insulin-independent manner.

7. Jeju pine  Fermentation broth ( Fermented Media of Jeju Scoria  : FMJS Effect on hepatic lipid accumulation in liver cells

 Insulin resistance, one of the etiologies of metabolic syndrome including diabetes, is known to induce excessive lipid accumulation (fatty liver) in liver tissue along with decreased glucose absorption in skeletal muscle cells. In this experiment, we investigated the effects of FMJS in the treatment of hepatic cells (HepG2) with free fatty acid (FFA) in the intracellular lipid accumulation (see FIG. 12).

Treatment of 0.1 mM FFA to HepG2 cells in culture increases the accumulation of intracellular lipid granules. However, the parallel accumulation of Jeju pine fermentation broth (FMJS) reduced the accumulation of lipids, and the effective concentration of Jeju pine fermentation broth (FMJS) was 0.6-1.2%.

8. Safety, Cytoprotective Effect and Glucose Transport Enhancement (Anti-diabetic) Activity on Cells of Crop Cultured with Fermented Media of Jeju Scoria (FMJS) (L6 Skeletal Muscle Cell )

 Experiments were performed with samples of culture crops using Jeju pine fermentation broth (FMJS) as a nutrient solution (see Table 3).

Fermentation broth (nutrient solution) for cultivation Hurum No. Sample No. Remarks HR-V-01-20 a
Sprout rape, Sprout cabbage, Sprout buckwheat, Sprout barley, Bibi Sprout HR-V-03-20 b HR-V-04-20 c HR-V-05-20 d HR-V-06-10 e HR-V-06-20 f

Each sample was diluted sequentially in the concentration range of 0-1,000 ug / ml, added to the cell culture medium (serum not added), and then cultured with L6 muscle cells for 72 hours, followed by LDH, MTT analysis, and H33342 fluorescence staining. The change in cell nuclear condensation was investigated.

No cytotoxicity was observed in all the low to high concentrations in all six samples (see Figures 13 and 14), of which four samples (a, b, c and e) were conversely prolonged (72 hours) in nutrient depletion media. It has been shown to protect cells from cytotoxicity when cultured.

A total concentration of 300 ug / ml, which is not cytotoxic in all six samples, was selected to investigate whether these extracts promote glucose transport in L6 skeletal muscle cells. Glucose transport by muscle cells was enhanced in six sample treatment groups, and the nutrient solution plants a and d showed the highest efficacy (see FIG. 15).

In summary, the extracts (6 total) of nutrient-cultivated crops from Jeju pine fermentation did not show toxicity to the cells, while four samples showed cytoprotective effects from nutrient deficient cell damage. In particular, all six extracts showed glucose transport enhancement effects, and a and d activities were relatively high.

Jeju fermentation broth containing many useful substances was prepared through the fermentation extraction technology of the useful components of Jeju pine using the microorganism of the present invention. It contains valuable ingredients such as zinc, which makes it possible to use the plant in various ways industrially.

Especially in the case of vanadium-rich plants, it is useful to prevent and treat diabetes, and in the case of plants containing lots of selenium, it is useful as anti-cancer effect, skin health and skin aging, and blood circulation. In the case of zinc-rich plants, it can be usefully used as a material related to hair loss and taste prevention.

1: container for nutrient solution cultivation 2: named plant seed
3: Jeju pine fermentation broth 4: Jeju pine
5: Plain stone

Claims (9)

Jeju fermentation broth extracted by fermentation of microorganisms, which is obtained by fermenting Jeju pine by using microorganisms, and used as a nutrient solution for plant nutrient cultivation. The method of claim 1,
The microorganism is Bacillus sp . With Streptomyces sp . To Jeju pine fermentation broth is extracted by microbial fermentation, characterized in that the single or mixed. Preparing a Jeju fermentation broth that is a composition for plant nutrient cultivation by fermenting the Jeju pine by using microorganisms (S10);
A soaking step (S20) of soaking the plant seeds in the fermentation broth prepared in step S10;
Nutrient cultivation step (S30) of cultivating the plant seeds called in the step (S20) using the Jeju pine fermentation broth prepared in the step (S10);
Nutrient cultivation method of the functional plant using the Jeju pine fermentation broth extracted by the microbial fermentation comprising a. The method of claim 3,
In the Jeju pine nutrient solution preparation step (S10) the microorganism Bacillus sp . And Streptomyces sp . To A nutrient solution cultivation method of a functional plant using Jeju pine fermentation broth extracted by microbial fermentation, characterized in that the mixture alone or mixed. The method of claim 3,
Seed of the plant in the soaking step (S20) is cultivated nutrient solution of the functional plant using the Jeju pine fermentation broth extracted by fermentation of microorganism fermentation, characterized in that soaked for 3 to 5 hours in the Jeju pine fermentation broth prepared in the step (S10) Way. A functional plant cultured in nutrient solution with Jeju pine fermentation broth extracted by the microorganism fermentation according to claim 1. A functional plant grown by the nutrient solution cultivation method of a functional plant using the Jeju pine fermentation broth extracted from the microorganism fermentation according to any one of claims 3 to 5. Functional material using a functional plant nutrient-cultivated in Jeju pine fermentation broth extracted by the microorganism fermentation according to claim 6. Functional material using a functional plant cultured nutrient solution using the Jeju pine fermentation broth extracted by the microorganism fermentation according to claim 7.

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