KR20130125020A - Composition of functional cosmetics using ecofriendly chamomile extract - Google Patents
Composition of functional cosmetics using ecofriendly chamomile extract Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
Abstract
Description
본 발명은 유기용매를 사용하지 않는 친환경 용매인 초임계 유체를 이용한 캐모마일 추출물을 함유한 기능성화장품 조성물에 관한 것으로 기존의 에탄올추출법에 의한 캐모마일추출법에 비하여 다량의 폴리페놀 함량과 플라보노이드 함량을 지녀 항산화효과가 탁월한 기능성 화장료 조성물에 관한 것이다. The present invention relates to a functional cosmetic composition containing a chamomile extract using a supercritical fluid, which is an environmentally friendly solvent that does not use an organic solvent, and has an antioxidative effect with a large amount of polyphenol and flavonoid content as compared to the chamomile extraction method by the conventional ethanol extraction method. Relates to an excellent functional cosmetic composition.
천연물질에 많은 유효성분들을 많이 함유하고 있고 이러한 약효성분을 추출하기 위한 많은 연구가 진행되어져 왔다. 천연물질에서 유효물질을 추출할 때 수열추출법을 많이 사용되고 있지만, 물의 끊는 점까지 가열하여 열에 약한 유효물질이 파괴될 수 있고, 물에 용해되지 않는 유효성분은 추출할 수 없는 단점을 지니고 있다. Many active ingredients are contained in natural substances and many researches have been conducted to extract these active ingredients. The hydrothermal extraction method is used a lot when extracting the active substance from natural substances, but the active substance that is weak to heat may be destroyed by heating up to the break point of water, and the active ingredient which does not dissolve in water has a disadvantage that it cannot be extracted.
천연물질에서 유효성분을 추출하기 위한 다른 방법으로 유기용매를 이용하는 추출방법이 있는데, 이것을 추출효율은 좋지만, 유기용매를 제거하는 공정이 요구되면, 잔존 유기용매로 인한 피부 부작용 문제가 대두하고 있다. There is an extraction method using an organic solvent as another method for extracting the active ingredient from natural substances, the extraction efficiency is good, but when the process of removing the organic solvent is required, there is a problem of skin side effects due to the remaining organic solvent.
이에, 본 발명자들은 유기용매를 사용하지 않으면서도 저온에서 추출할 수 있는 초임계 이산화탄소를 이용하여 캐모마일추출물에서 유효성분을 추출하고자 하였다. 초임계 추출방법은 저온에서 작업하므로 유효성분이 파괴되지 않으며 잔존 유기용매로 인한 피부 부작용 문제도 없으며, 유효성분을 보다 효율적으로 추출할 수 있는 장점을 지니고 있고, 항산화효과도 좋은 것을 확인하고, 본 발명을 완성하였다.
Thus, the present inventors tried to extract the active ingredient from the chamomile extract using supercritical carbon dioxide that can be extracted at low temperatures without using an organic solvent. Since the supercritical extraction method works at low temperature, the active ingredient is not destroyed and there is no problem of skin side effects due to the remaining organic solvent, and it has the advantage of extracting the active ingredient more efficiently, and it is confirmed that the antioxidant effect is good, and the present invention Was completed.
본 발명에서는 물의 끊는 점까지 가열하여 열에 약한 유효물질이 파괴될 수 있고, 물에 용해되지 않는 유효성분은 추출할 수 없는 단점을 지닌 수열추출법을 보강하고, 잔존 유기용매로 인해 피부 부작용이 발생하는 용매추출방법의 문제를 초임계 유체를 이용하여 해결하고자 하였다.
In the present invention, by heating up to the break point of the water, the active material that is weak to heat can be destroyed, and the active ingredient which is insoluble in water cannot be extracted, and reinforces the hydrothermal extraction method, and the skin side effects are caused by the remaining organic solvent. The problem of solvent extraction method was solved using supercritical fluid.
이에, 본 발명자들은 유기용매를 사용하지 않으면서도 저온에서 추출할 수 있는 초임계 이산화탄소를 이용하여 캐모마일추출물에서 유효성분을 추출하고자 하였다. 초임계 추출방법은 저온에서 작업하므로 유효성분이 파괴되지 않으며 잔존 유기용매로 인한 피부 부작용 문제도 없으며, 유효성분을 보다 효율적으로 추출할 수 있는 장점을 지니고 있고, 항산화효과도 좋은 것을 확인하고, 본 발명을 완성하고자 하였다.Thus, the present inventors tried to extract the active ingredient from the chamomile extract using supercritical carbon dioxide that can be extracted at low temperatures without using an organic solvent. Since the supercritical extraction method works at low temperature, the active ingredient is not destroyed and there is no problem of skin side effects due to the remaining organic solvent, and it has the advantage of extracting the active ingredient more efficiently, and it is confirmed that the antioxidant effect is good, and the present invention To complete.
본 발명에서는 캐모마일잎을 이용하여 에탄올추출법과 초임계 이산화탄소 추출법을 실시하여 각각 캐모마일추출물을 얻었고, 초임계 이산화탄소를 이용한 추출법이 더욱 많은 폴리페놀함량과 플라보노이드 함량은 나타내며, 더 좋은 항산화효과를 나타낼 수 있었다.
In the present invention, the ethanol extraction method and the supercritical carbon dioxide extraction method were used to extract the chamomile extract, respectively. .
본 발명은 에탄올추출법과 초임계 이산화탄소 추출법을 이용하여 캐모마일추출물을 얻고, 각각의 캐모마일추출물에서 폴리페놀과 플라보노이드 함량을 측정하고, 항산화효과를 측정하였다. In the present invention, chamomile extracts were obtained using ethanol extraction and supercritical carbon dioxide extraction, polyphenol and flavonoid contents were measured in each chamomile extract, and antioxidant effects were measured.
본 발명의 비교대상인 에탄올 캐모마일추출물의 제조는 다음과 같다. 먼저, 캐모마일잎을 정제수로 세척하고 음지에서 자연 건조시킨 후 분쇄기를 이용하여 분쇄한다. 분쇄된 캐모마일잎을 에탄올에 넣고 추출한 후 3회 반복하여 여과한다. 열수추출법나 유기용매 추출법보다 에탄올 추출법을 선택한 이유는 열수추출법에서는 물에 용해되는 유효성분이 작고, 끊는 물에 의해 유효성분이 파괴될 수 있는 위험성이 있고, 유기용매는 추출효율은 좋지만 잔존 유기용매로 인한 부작용이 우려되기 때문이다. 여과물을 감압 농축하여 캐모마일 에탄올추출물을 얻는다. Preparation of the ethanol chamomile extract of the present invention is as follows. First, the chamomile leaves are washed with purified water and naturally dried in the shade, and then ground using a grinder. The crushed chamomile leaves are put in ethanol and extracted and filtered three times. The reason for choosing the ethanol extraction method rather than the hot water extraction method or the organic solvent extraction method is that there is a risk that the active ingredient dissolved in water is small in the hot water extraction method and the active ingredient is destroyed by the water which is broken, and the organic solvent has good extraction efficiency, This is because side effects are a concern. The filtrate was concentrated under reduced pressure to obtain a chamomile ethanol extract.
본 발명의 초임계 이산화탄소 캐모마일 추출물의 제조는 다음과 같다. 먼저, 캐모마일잎을 정제수로 세척하고 음지에서 자연 건조한 후 분쇄기를 이용하여 분쇄한다. 분쇄된 캐모마일 잎을 반응기에 넣고 섭시 38도에서 이산화탄소를 주입하여 200기압까지 압력을 높인다. 시간은 4시간 동안 유지하고, 압력을 1기압으로 낮추면 이산화탄소에 용해된 캐모마일추출물을 얻는다. Preparation of supercritical carbon dioxide chamomile extract of the present invention is as follows. First, the chamomile leaves are washed with purified water and naturally dried in the shade, and then ground using a grinder. Put the crushed chamomile leaves in the reactor and injects carbon dioxide at 38 degrees Celsius to increase the pressure up to 200 atm. The time is maintained for 4 hours, and the pressure is lowered to 1 atmosphere to obtain chamomile extract dissolved in carbon dioxide.
본 발명에 따는 초임계 이산화탄소 캐모마일 추출물은 더욱 많은 폴리페놀함량과 플라보노이드 함량은 나타내며, 더 좋은 항산화효과를 나타낼 수 있음을 확인하여 본 발명을 완성하고자 하였다. Supercritical carbon dioxide chamomile extract according to the present invention was shown to be more polyphenol content and flavonoid content, was to complete the present invention was confirmed that can exhibit a better antioxidant effect.
본 발명의 자외선 차단제의 기능성 화장료 조성물은 통상적으로 제조되는 로션, 크림 제형으로 제조될 수 있다. 이하 본 발명의 이해를 돕기 위하여 바람직한 실시한 예를 제시한다. 그러나 하기의 실시한 예는 본 발명을 더욱 쉽게 이해하기 위하여 제공되는 것일 뿐, 실시한 예에 의해 본 발명의 내용이 한정되는 것은 아니다.
The functional cosmetic composition of the sunscreen of the present invention can be prepared in a conventional lotion or cream formulation. Hereinafter, preferred examples are provided to help understanding of the present invention. However, the following examples are provided to further understand the present invention, and the present invention is not limited by the examples.
실시 비교예 1 : 에탄올 캐모마일 추출물의 제조Example 1 Preparation of Ethanol Chamomile Extract
캐모마일 잎을 정제수로 세척하고 음지에서 자연 건조한 후 분쇄기를 이용하여 2~3 cm의 크기로 분쇄한다. 분쇄된 캐모마일 1kg을 에탄올 9L에 넣고 15 내지 25 ℃에서 24시간 동안 추출하였다. 상기 추출물을 와트만 3번 여과지로 여과하고 나서, 냉각 콘덴서가 달리 추출장치를 이용하여 30 내지 40 ℃에서 감압 농축하여 에탄올 캐모마일추출물을 40.5 g을 얻었다.
The chamomile leaves are washed with purified water, dried naturally in the shade, and then ground to a size of 2-3 cm using a grinder. 1 kg of crushed chamomile was placed in 9 L of ethanol and extracted for 24 hours at 15 to 25 ℃. The extract was filtered using Whatman No. 3 filter paper, and the cooling condenser was concentrated under reduced pressure at 30 to 40 ° C. using an extractor to obtain 40.5 g of ethanol chamomile extract.
실시 제조예 1 : 초임계 이산화탄소 캐모마일 추출물의 제조Example 1 Preparation of Supercritical Carbon Dioxide Chamomile Extract
캐모마일 잎을 정제수로 세척하고 음지에서 자연 건조한 후 분쇄기를 이용하여 2~3 cm의 크기로 분쇄한다. 분쇄된 캐모마일 잎 1Kg을 10L 반응기에 넣고 섭시 38도에서 이산화탄소를 주입하여 200기압까지 압력을 높인다. 시간은 4시간 동안 유지하고, 압력을 1기압으로 낮추면 이산화탄소에 용해된 43.2 g을 얻었다.
The chamomile leaves are washed with purified water, dried naturally in the shade, and then ground to a size of 2-3 cm using a grinder. Put 1Kg of crushed chamomile leaves into a 10L reactor and inject carbon dioxide at 38 degrees Celsius to increase the pressure to 200 atm. The time was maintained for 4 hours and the pressure was lowered to 1 atm to give 43.2 g dissolved in carbon dioxide.
실시예 1. 추출물의 총 폴리페놀 함량 측정Example 1. Determination of Total Polyphenol Content of Extracts
에탄올 캐모마일추출물과 초임계 캐모마일추출물 폴리페놀 화합물의 함량은 Folin-Denis법을 변형하여 측정하였다. Methanol에 1 ㎎/㎖ 농도로 용해시킨 시료액 80 ㎕와 Folin-Denis reagent 80 ㎕를 혼합하여 3분간 반응시킨 뒤 10% Na2CO3 80 ㎕를 혼합하여 60분 동안 암실에서 반응시킨 후, 상등액 120 ㎕를 취하여 96 well plate에 옮겨 700 ㎚에서 흡광도를 측정하였다. 표준 곡선은 표준물질인 tannic acid를 이용하여 polyphenol의 함량을 구하였다. 에탄올 캐모마일 추출물의 총 폴리페놀의 함량은 1071.5 mg/100g이 반면, 초임계 이산화탄소 캐모마일 추출물의 총 폴리페놀 함량은 1543.4 mg/100g으로 더 많은 폴리페놀 함량을 지니고 있다.
The contents of ethanol chamomile extract and supercritical chamomile extract polyphenol compounds were measured by modifying the Folin-Denis method. 80 μl of the sample solution dissolved in 1 mg / ml in methanol and 80 μl of Folin-Denis reagent were mixed and reacted for 3 minutes. Then, 80 μl of 10% Na 2 CO 3 was mixed and reacted in the dark for 60 minutes. 120 μl was taken and transferred to a 96 well plate, and the absorbance was measured at 700 nm. The standard curve was calculated from polyphenol content using tannic acid as a standard. The total polyphenol content of the ethanol chamomile extract is 1071.5 mg / 100 g, while the total polyphenol content of the supercritical carbon dioxide chamomile extract is 1543.4 mg / 100 g, which has more polyphenol content.
실시예 2. 추출물의 총 플라보노이드 함량 측정Example 2. Determination of Total Flavonoid Content of Extracts
에탄올 캐모마일추출물과 초임계 캐모마일추출물의 플라보노이드 함량은 Davis 등의 방법을 변형하여 측정하였다. 1 ㎎/㎖ 농도로 methanol에 용해시킨 시료액 10 ㎕와 1N-NaOH 10㎕, dietyleneglycol 200 ㎕를 혼합하여 37℃ slide warmer에서 60분 동안 반응시킨 후 420 ㎚에서 흡광도를 측정하였다. 표준곡선은 표준물질 rutin을 이용하여 flavonoid 함량을 구하였다. 에탄올 캐모마일 추출물의 총 플라보노이드의 함량은 358.5 mg/100g이 반면, 초임계 이산화탄소 캐모마일 추출물의 총 플라보노이드 함량은 550.4 mg/100g으로 더 많은 플라보노이드 함량을 지니고 있다.
Flavonoid contents of ethanol chamomile extract and supercritical chamomile extract were measured by modifying the method of Davis et al. 10 μl of the sample solution dissolved in methanol at a concentration of 1 mg / ml, 10 μl of 1N-NaOH, and 200 μl of dietyleneglycol were mixed and reacted at 37 ° C. slide warmer for 60 minutes, and then absorbance was measured at 420 nm. The standard curve was calculated from flavonoid content using the standard rutin. The total flavonoid content of the ethanol chamomile extract was 358.5 mg / 100 g, while the total flavonoid content of the supercritical carbon dioxide chamomile extract was 550.4 mg / 100 g, which has more flavonoid content.
실시예 3 : 항산화효과 측정Example 3 Antioxidant Effect Measurement
항산화효과의 대표적인 측정방법인 수포옥사이드 디스뮤타아제 (superoxidase dismutase, SOD) 유사활성을 측정하였다. SOD 유사활성은 측정방법은 다음과 같다. 시료용액인 각각 에탄올 캐모마일추출물, 초임계 이산화탄소 캐모마일추출물 0.2 ml에 Tris-HCl 완충용액(pH 8.5) 3 ml와 7.2 mM 파이로겔롤 (pyrogallol) 0.2 ml 을 가하고 10초간 와류혼합 (vortex mix) 후 25 ℃에서 10분간 반응시킨 후 1 M HCl 1 ml를 가하여 반응을 정지시키고 420 nm에서 흡광도로 산화된 파이로겔롤 양을 측정하였다. SOD 유사활성은 시료 첨가군과 무 첨가군의 흡광도에서 산화된 파이로겔롤 차이로 나타내었다.Superoxidease dismutase (SOD) -like activity, a representative measure of antioxidant effect, was measured. SOD-like activity is measured as follows. Add 0.2 ml of Tris-HCl buffer (pH 8.5) and 0.2 ml of 7.2 mM pyrogallol to 0.2 ml of ethanol chamomile extract and supercritical carbon dioxide chamomile extract as sample solutions, and then vortex mix for 10 seconds. After reacting for 10 minutes at 1 ° C., 1 ml of 1 M HCl was added to stop the reaction, and the amount of pyrogelol oxidized by absorbance at 420 nm was measured. SOD-like activity was expressed by the difference of oxidized pyrogelol in absorbance of the sample addition group and no addition group.
SOD유사활성도(%) =
SOD-like activity (%) =
(무첨가군 파이로겔롤 양 - 시료첨가군 파이로겔롤 양)(No additive group pyrogelol amount-Sample addition group pyrogelol amount)
---------------------------------------------------- ×100-------------------------------------------------- -× 100
무첨가군 파이로겔롤 양 Additive-free pyrogelol sheep
에탄올 캐모마일 추출물의 SOD 유사활성을 측정한 결과는 65.5%이며, 초임계 이산화탄소 캐모마일 추출물은 87.6%를 나타내어 훨씬 강한 항산화효과를 나타내었다.
The SOD-like activity of ethanol chamomile extract was 65.5%, and supercritical carbon dioxide chamomile extract was 87.6%, which showed much stronger antioxidant effect.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20180071558A (en) | 2016-12-20 | 2018-06-28 | 이호규 | Feminine care composition for woman containing extract of chamomile flower and manufacturing method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20180071558A (en) | 2016-12-20 | 2018-06-28 | 이호규 | Feminine care composition for woman containing extract of chamomile flower and manufacturing method thereof |
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