KR20130119378A - Method and device for detecting target material - Google Patents

Abstract

PURPOSE: A method and a device for detecting a target material are provided to detect a target material by applying an electric field or a magnetic field to particles on which a detection reactor coupling to a target material is formed, thereby rapidly and simply detecting a target material. CONSTITUTION: A method for detecting a target material comprises the following steps of mixing an inspection target material with the solvent in which multiple particles (910) on which a detection reactor (920) which is selectively coupled to a target material (930) is formed are dispersed; and applying an electric field or a magnetic field to the solvent in which the inspection target material is mixed. If the target material is included in the inspection target material, any one characteristic of the hydrodynamic size, the shape, the surface charge, and the mass of the multiple particles is changed by the target material coupled to the detection reactor. The multiple particles with the changed characteristic are regularly arranged at particular intervals by being applied with the electric field or the magnetic field, and reflect or transmit the light in the range of the particular wavelength. [Reference numerals] (AA) Electric field or a magnetic field; (BB,DD) Incident light; (CC) Reflected light 1; (EE) Reflected light 2

Description

METHOD AND DEVICE FOR DETECTING TARGET MATERIAL}

The present invention relates to a method and apparatus for detecting a target substance. More specifically, in combination with the target material, at least one of hydrodynamic size, shape, surface charge, and mass changes, and thus the arrangement of the reflected light or transmitted light is changed by changing the applied state by the applied electric or magnetic field. The present invention relates to a method and apparatus for easily detecting a target material using particles having different wavelengths.

Numerous studies have been conducted around the world until recently regarding nucleic acids such as deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), proteins, cells, bacteria, viruses, etc. Its importance is increasing day by day. Even in the actual medical environment, there is a continuous demand for the development of a technology capable of quickly and accurately identifying the presence or type of a disease by detecting substances related to human disease treatment such as nucleic acids, proteins, bacteria, and viruses.

However, the conventional detection techniques introduced to date have a problem that most of them require expensive reagents and complicated methods of use, or require inspectors to have specialized knowledge. There was also the problem of having to visit the inspection station in person.

To solve these problems, factors such as simple usage, wide range of detectable target materials, high portability, and low price should be considered. As an example of an improved detection technique, methods using ion, affinity, size and immunochromatography are commonly used.

Among them, the method using the principle of membrane-based immunochromatography is widely used in the field of medical diagnosis as a method having high sensitivity, wide selectivity, and portability. According to this method, the detection reactor is connected to the surface of the gold nanoparticles to determine whether the target material is detected based on whether the red lines characteristic of the gold nanoparticles appear on the sample line and the control line.

MICT (Magnetic Immuno Chromatographic Test) method using magnetic particles corresponds to a method in which magnetic nanoparticles are applied in place of gold nanoparticles in immunochromatography, and replaces expensive gold nanoparticles with magnetic particles. It also has the advantage of reducing measurement time and improving sensitivity.

However, the methods described above still have a problem in that a separate high performance measurement apparatus is required to confirm the detection result.

Accordingly, the present inventors have developed a method and apparatus capable of detecting a target material using a photocrystalline material whose color or light transmittance changes as an electric or magnetic field is applied.

The present invention provides a method and apparatus for detecting a target substance which is faster, simpler and more sensitive than conventional detection techniques by detecting an target substance by applying an electric or magnetic field to a particle having a detection reactor capable of bonding with the target substance. It aims to do it.

In the target material detection method according to the present invention, a detection reactor capable of selectively binding to a target material includes mixing a test target material with a solvent in which a plurality of particles formed on a surface are dispersed, and a solvent in which the test target material is mixed. And applying an electric or magnetic field to the target material, wherein the target material is combined with the detection reactor, thereby providing a hydrodynamic size and shape of the plurality of particles. At least one of the surface charge and the mass is changed, and the plurality of particles having the changed property are regularly arranged at a specific range of intervals as the electric or magnetic field is applied to reflect or transmit light in a specific wavelength range. It is characterized by.

The target material may include at least one of a protein, DNA, RNA, cells, bacteria, and viruses.

The detection reactor may include at least one of nickel (Ni), a carboxyl group (-COOH), an amine group (-NH ₂ ), and a hydroxyl group (-OH).

The detection reactor may include at least one of Protein A, Protein G, Streptavidin, Anti-AFP, and Anti-DEP.

The particles may comprise a superparamagnetic material.

The solvent may be made of a visible light transmitting material.

The solvent may include at least one of a polymer in a network structure and a solid in a gel form.

The target material detection method may further include determining whether the target material is included in the test target material by referring to a wavelength of light reflected from the solvent to which the electric or magnetic field is applied.

In addition, the target material detection apparatus according to the present invention includes a display unit in which a plurality of particles formed on a surface of a detection reactor capable of selectively binding to a target material are regularly arranged, and into which a test target material may be injected; And an electromagnetic field generator configured to apply an electric field or a magnetic field to the display unit, wherein the target material is combined with the detection reactor through the detection reactor when the target material is included in the inspection target material. Properties of at least one of the hydrodynamic size, shape, surface charge, and mass are changed, and the plurality of particles having the changed properties are arranged regularly at specific intervals as the electric or magnetic field is applied, and at a specific wavelength. It is characterized by reflecting or transmitting light in the range.

According to the present invention, anyone can easily target the target material through the color change of the reflected light or transmitted light which can be easily detected compared to the existing methods and devices, the detection device can be further miniaturized, and can be observed with the naked eye without additional discriminating means. The effect of being able to confirm the presence or absence of the detection of a substance is achieved.

In addition, according to the present invention, since it is easier to modify the surface of the particle compared to the membrane-based immunochromatography method using the conventional gold nanoparticles, the target material detectable by forming a variety of detection reactants on the surface of the particle The effect of being able to extend the range of and detecting a target substance with high sensitivity even with a small amount of samples is achieved.

FIG. 1 is a diagram exemplarily illustrating a principle of controlling a wavelength of light reflected from a color variable material according to an exemplary embodiment of the present invention.
FIG. 2 is a diagram exemplarily illustrating a result of photographing a color change of a color-variable material that appears when electric or magnetic fields of various intensities are applied according to an embodiment of the present invention.
FIG. 3 is a diagram exemplarily illustrating a graph measuring wavelengths of light reflected from a color variable material according to an intensity of an electric or magnetic field according to an exemplary embodiment of the present invention.
4A is a diagram exemplarily illustrating an SEM image of the magnetic particles constituting the color variable material according to the exemplary embodiment of the present invention. FIG. 4B is a view showing that the green variable light is reflected by encapsulating the color variable material according to the exemplary embodiment of the present invention into a capsule made of a light transmitting material and then applying a magnetic field.
FIG. 5 illustrates a magnet in which a butterfly pattern is formed on an upper portion of the color variable material, and a magnetic pole alternately formed in a stripe shape to generate magnetic fields of different intensities in the lower portion of the color variable material according to an embodiment of the present invention; After positioning, the color and pattern of the color-variable material change as the magnet rotates.
FIG. 6 is a diagram exemplarily illustrating a principle of adjusting light transmittance of a light transmittance variable material according to an exemplary embodiment of the present invention.
7 and 8 are diagrams exemplarily showing results of experiments of applying a magnetic field in a state in which particles are dispersed in a solvent according to one embodiment of the present invention.
9 is a diagram conceptually illustrating an operating principle of a target material detection apparatus using a color variable material or a light transmittance variable material according to an embodiment of the present invention.
10 and 11 are diagrams exemplarily illustrating a configuration of a target substance detection apparatus according to an embodiment of the present invention.
12 is a diagram illustrating a flowchart of a method for detecting a target substance according to an embodiment of the present invention.

DETAILED DESCRIPTION OF THE INVENTION The following detailed description of the invention refers to the accompanying drawings that show, by way of illustration, specific embodiments in which the invention may be practiced. These embodiments are described in sufficient detail to enable those skilled in the art to practice the invention. It should be understood that the various embodiments of the present invention are different, but need not be mutually exclusive. For example, certain features, structures, and characteristics described herein may be implemented in other embodiments without departing from the spirit and scope of the invention in connection with an embodiment. It is also to be understood that the position or arrangement of the individual components within each disclosed embodiment may be varied without departing from the spirit and scope of the invention. The following detailed description is, therefore, not to be taken in a limiting sense, and the scope of the present invention is to be limited only by the appended claims, along with the full scope of equivalents to which such claims are entitled, if properly explained. In the drawings, like reference numerals refer to the same or similar functions throughout the several views.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings, in order that those skilled in the art can easily carry out the present invention.

[Configuration of Particles]

Particles according to an embodiment of the present invention may be present in a colloidal solvent dispersed as a particle having a negative charge or a positive charge. At this time, the particles may be arranged at a predetermined interval from each other due to mutual repulsive force due to the charge of the same sign. The diameter of the particles may be several nm to several hundred μm, but is not necessarily limited thereto.

In addition, the particles according to an embodiment of the present invention, may be configured in the form of a core-shell (core-shell) consisting of different materials, multi-core (multi-core) consisting of different materials And, it may be configured in the form of a cluster consisting of a plurality of nanoparticles, in addition, a charge layer having a predetermined charge may be configured in a structure surrounding these particles.

More specifically, the particles according to an embodiment of the present invention is silicon (Si), titanium (Ti), barium (Ba), strontium (Sr), iron (Fe), nickel (Ni), cobalt (Co), lead (Pb), aluminum (Al), copper (Cu), silver (Ag), gold (Au), tungsten (W), molybdenum (Mo) and the like or a material containing an oxide thereof. In addition, the particles according to an embodiment of the present invention may be made of a polymer material such as PS (polystyrene), PE (polyethylene), PP (polypropylene), PVC (polyvinyl chloride), PET (polyethylen terephthalate). In addition, the particles according to an embodiment of the present invention may be configured as a form in which a material having a charge on a particle or a cluster that does not have a charge, for example, the surface is formed by an organic compound having a hydrocarbon group Particles processed (or coated) by organic compounds having processed (or coated) particles, carboxylic acid groups, ester groups, or acyl groups, halogens (F, Cl, Br) , I, etc.) Particles whose surface is processed (coated) by complex compounds containing elements, particles whose surface is processed (coated) by coordination compounds containing amines, thiols, and phosphines For example, the particles may be charged by forming radicals on their surfaces.

On the other hand, according to an embodiment of the present invention, in order for the particles to maintain a stable colloidal state without being precipitated in the solvent to be described later and to exhibit photonic crystallinity effectively, the interelectrokinetic potential of the colloidal solution of the particles and the solvent (electrokinetic potential) (Ie, zeta potential) may be higher than or equal to a predetermined value, a difference in specific gravity between particles and a solvent may be lower than or equal to a predetermined value, and a difference in refractive index between the solvent and the particle may be greater than or equal to a predetermined value. For example, the absolute value of the interfacial potential of the colloidal solution may be 10mV or more, the difference in specific gravity of the particles and the solvent may be 5 or less, the difference in refractive index of the particles and the solvent may be 0.3 or more.

On the other hand, according to an embodiment of the present invention, the particles may include a material that becomes magnetic, that is, magnetized as the magnetic field is applied. Particularly, according to an embodiment of the present invention, when an external magnetic field is applied to prevent a phenomenon of aggregation of particles having magnetism in the case where a magnetic field is not externally applied, magnetization occurs but an external magnetic field is not applied A superparamagnetic material which does not cause remnant magnetization can be used.

Also, according to one embodiment of the present invention, in order to prevent the particles from being well dispersed in the solvent and agglomerate, the surface of the particles can be coated with the charge of the same sign, It may be coated with a material having a different specific gravity from that of the particles, or a material having a specific gravity different from that of the particles may be mixed with the solvent.

In addition, according to one embodiment of the invention, the particles may be configured to reflect light of a particular wavelength, that is, to have a specific color. More specifically, the particles according to the present invention may have a specific color through oxidation control or coating of inorganic pigments, pigments and the like. For example, Zn, Pb, Ti, Cd, Fe, As, Co, Mg, Al and the like including a chromophore may be used in the form of oxides, emulsions and lactates as inorganic pigments coated on the particles according to the present invention , A fluorescent dye, an acid dye, a basic dye, a mordant dye, a sulfide dye, a bat dye, a disperse dye, a reactive dye and the like may be used as the dye coated on the particles according to the present invention.

In addition, according to one embodiment of the present invention, the particles may be coated with a material having a charge to have the same charge with each other.

[Electric Polarization Characteristics of Particles or Solvents]

According to an embodiment of the present invention, the particles or the solvent included in the display device may have an electrical polarization characteristic. The particles or the solvent may have an electron polarization, an ion polarization, an interfacial polarization, and an external electric field. It may include a material that is polarized by any one of the rotational polarization.

More specifically, the particles or the solvent maintain the electrical equilibrium when no external electric field is applied, but the particles may be polarized as the electrons in the particles or the solvent move in a predetermined direction when the external electric field is applied. Conversely, when no external electric field is applied, each particle is randomly arranged by unit polarization generated by electrically asymmetrical components constituting the particle or solvent, but when an external electric field is applied Particles or solvents with unit polarization may be rearranged in a predetermined direction depending on the direction of the external electric field, and thus may exhibit a fairly large polarization value as a whole. Meanwhile, according to an embodiment of the present invention, unit polarization may occur in an asymmetrical arrangement of ions or in an asymmetric structure of molecules, and even in the absence of an external electric field due to such unit polarization, a fine residual polarization may be achieved. value) may appear.

According to one embodiment of the present invention, unit polarization characteristics may be exhibited by asymmetrical arrangement of molecules. More specifically, in addition to water molecules, trichloroethylene, carbon tetrachloride, di-iso-propyl ether, toluene, methyl-t-bithyl ether, xylene, benzene, diethyl ether, dichloromethane, 1,2-dichloroethane, butyl acetate, iso-propanol, n-Butanol, Tetrahydrofuran, n-Propanol, Chloroform, Ethyl Acetate, 2-Butanone, Dioxane, Acetone, Metanol, Ethanol, Acetonitrile, Acetic Acid, Dimethylformamide, Dimethyl Sulfoxide, etc. Therefore, it may be employed as a material constituting the particles or solvent according to the present invention. For reference, a polarity index used to compare polarization properties of materials is an index indicating relative polarization degree of the material when the polarization property of water (H ₂ O) is set to 9.

On the other hand, the particles or the solvent according to an embodiment of the present invention is a ferroelectric material in which the polarization amount increases as the external electric field is applied and the residual polarization amount is large even when the external electric field is not applied and the hysteresis remains. It may include a superparaelectric material in which the polarization amount increases as the external electric field is applied and the residual polarization amount does not appear and no hysteresis is left when the external electric field is not applied.

In addition, the particles or the solvent according to an embodiment of the present invention may include a material having a perovskite structure, a material having a perovskite structure, such as ABO ₃ PbZrO ₃ , PbTiO ₃ , Pb (Zr, Ti) O ₃ , SrTiO ₃ BaTiO ₃ , (Ba, Sr) TiO ₃ , CaTiO ₃ , LiNbO ₃ , and the like can be given. For example, PbZrO ₃ (or PbTiO ₃₎ PbZrO depending on the direction of the external electric field is applied to the _third (or PbTiO ₃₎ in the Zr (or Ti) is located (that is, B of the ABO ₃ structure) may vary As a result, the polarity of the entire PbZrO ₃ (or PbTiO ₃ ) may be changed.

Meanwhile, according to one embodiment of the present invention, the solvent may include a material having a polarity index of 1 or more.

However, the configuration of the particles and the solvent according to the present invention is not necessarily limited to those listed above, but within the range in which the object of the present invention can be achieved, that is, within the range in which the spacing between the particles can be controlled by an electric field. Note that changes can be made accordingly.

[Configuration of Detection Reactor]

According to one embodiment of the invention, a detection reactor capable of binding with a given target material may be formed on the surface of the particles. According to one embodiment of the invention, the particles and the detection reactor can be conjugated (conjugated) by covalent bonds to form a particle complex.

As will be described later, if the target material is included in the test target material mixed with the particles and the solvent, the hydrodynamic size, shape, surface charge and mass of the particles due to binding of the target material to the detection reactor formed on the surface of the particle At least one of the characteristics is changed, and the plurality of particles in which the characteristics are changed are regularly arranged at predetermined intervals as an electric or magnetic field is applied to reflect or transmit light in a specific wavelength range.

According to an embodiment of the present invention, as a detection reactor, a functional group having a charge such as a carboxyl group (-COOH), an amine group (-NH2), and a hydroxyl group (-OH) may be used, and nickel (Ni) and protein A ( Various antibodies can be used including Protein A), Protein G, Streptavidin, Anti-AFP, Anti-DEP. As a result, the detection reactor may be combined with various substances such as nucleic acids, proteins, cells, bacteria, viruses, and the like to perform detection functions necessary for various biological diagnosis such as ovulation, diabetes, heart disease, cancer, as well as purification.

[Configuration of Color Variable Material]

FIG. 1 is a diagram exemplarily illustrating a principle of controlling a wavelength of light reflected from a color variable material according to an exemplary embodiment of the present invention.

According to an embodiment of the present invention, when an electric field is applied to a plurality of particles 110 having the same charges, electrical attraction in a predetermined direction may be applied to the particles 110 due to the charge or magnetism of the particles 110. Alternatively, magnetic attraction acts and thus the distance between the particles 110 biased to one side becomes narrow, and at the same time, electrical repulsive force is applied between the particles 110 according to Coulomb's law (the particles have the same charge). In this case, the physical repulsive force due to steric hindrance is applied (when the hydrodynamic size of the particle is large due to a detection function or a target substance attached to the surface of the particle). Accordingly, the spacing of the particles 110 may be determined according to the relative strength of the repulsive force between the particles and the attractive force due to the electric or magnetic field, so that the particles 110 arranged at a predetermined interval may function as a photonic crystal. do. That is, according to the Bragg's law, since the wavelength of the light reflected from the particles 110 is determined by the interval of the particles 110, the wavelength of the light reflected from the particles 110 is controlled by controlling the interval of the particles 110 It can be adjusted.

Referring to FIG. 1, when an electric or magnetic field is not applied, the particles 312 in the capsule 130 may be irregularly arranged, and in this case, a different color may not be expressed from the particles 110. Next, when a predetermined electric or magnetic field is applied, the repulsive force between the particle 110 and the attraction force due to the electric or magnetic field is balanced, the particles 110 can be arranged regularly at a predetermined interval, accordingly It is possible to reflect light of a specific wavelength from the plurality of particles 110 whose spacing is controlled. In addition, since the electric field applied to the particle 110 or the intensity of the magnetic field increases, the attraction of the electric field or the magnetic field also increases, so that the distance between the particles 110 becomes narrower, and thus the wavelength of the light reflected from the particle 110 is further increased. Will be shortened. That is, according to one embodiment of the present invention, by adjusting the intensity of the electric or magnetic field applied to the particle 110 it is possible to adjust the wavelength of the light reflected from the particle (110). As the intensity of the electric or magnetic field increases, when the wavelength of the light reflected from the particle corresponds to the ultraviolet band beyond the visible light band, the particles transmit the light without reflecting the visible light.

Meanwhile, according to an embodiment of the present invention, as shown in FIG. 1, the color variable material composed of the particles 110 and the solvent 120 may be encapsulated by the capsule 130 composed of a light transmissive material. have.

FIG. 2 is a diagram exemplarily illustrating a result of photographing a color change of a color-variable material that appears when electric or magnetic fields of various intensities are applied according to an embodiment of the present invention.

Referring to FIG. 2, it can be seen that as the intensity of the applied electric or magnetic field is adjusted, the light reflected from the particles can be adjusted in all regions of the visible wavelength range from red to green and purple.

3 is a graph measuring wavelengths of light reflected from a color-variable material according to the intensity of an electric or magnetic field according to an embodiment of the present invention. It can be seen that the light gradually shifts to a blue light having a short wavelength.

4A is a diagram exemplarily illustrating an SEM image of the magnetic particles constituting the color variable material according to the exemplary embodiment of the present invention. In Fig. 4, superparamagnetic Fe ₃ O ₄ particles between 50 and 300 nm were used as the particles.

Figure 4 (b) is a view showing the green light is reflected after the encapsulated color variable material according to an embodiment of the present invention in a capsule made of a light transmitting material, by applying a magnetic field. Referring to FIG. 4 (b), it can be seen that the particles in the capsule are regularly arranged at regular intervals according to the magnetic field, and accordingly the light of the green series having a specific wavelength range is mainly reflected.

FIG. 5 illustrates a butterfly pattern formed on an upper portion of the color variable material and a magnet alternately formed in a stripe shape on a lower portion of the color variable material according to an embodiment of the present invention. Thereafter, the color and pattern of the color-variable material change as the magnet rotates.

On the other hand, according to the first embodiment of the present invention, when the electric field is applied to the particles and the solvent in a state in which a plurality of particles having the same charge charge is dispersed in a solvent having electrical polarization characteristics, the plurality of particles due to the charge of the particles In the particles of, electric attractive force proportional to the electric field intensity and the amount of charge of the particles are applied. Accordingly, the plurality of particles are electrophoresis and move in a predetermined direction, thereby narrowing the spacing between the particles. As the spacing is narrowed, the electrical repulsive force generated between the plurality of particles having the same sign with each other increases, so that the spacing between the particles does not continue to be narrowed, but a predetermined balance is achieved. On the other hand, due to the electrical polarization characteristics of the solvent, the solvent is polarized in a predetermined direction, thereby causing an electrical attraction locally, so that the interval between the plurality of particles electrically interacting with the polarized solvent is maintained at a predetermined interval. It becomes possible. That is, according to the first embodiment of the present invention, the electrical attraction due to the external electric field, the electrical repulsive force between the particles having the same code charge and the electrical attraction due to the polarization of the plurality of particles at a distance forming an equilibrium (equilibrium) Can be arranged regularly. According to the above principle, the spacing between the particles can be controlled at a predetermined interval, and the plurality of particles arranged at a predetermined interval can function as a photonic crystal. Since the wavelength of light reflected from the plurality of particles arranged regularly is determined by the spacing between the particles, the wavelength of the light reflected from the plurality of particles can be arbitrarily controlled by controlling the spacing between the particles. Here, the pattern of the wavelength of the reflected light varies depending on factors such as the intensity and direction of the electric field, the size and mass of the particles, the refractive index of the particles and the solvent, the amount of charge of the particles, the electrical polarization characteristics of the solvent, and the concentration of the dispersed particles in the solvent. May appear.

Further, according to the second embodiment of the present invention, when an electric field is applied to the particles and the solvent in a state in which a plurality of particles having the same charge and having the electrical polarization property are dispersed in the solvent, the plurality of particles due to the charge of the particles In the particles of, electric attractive force proportional to the electric field intensity and the amount of charge of the particles are applied. Accordingly, the plurality of particles are electrophoresis and move in a predetermined direction, thereby narrowing the spacing between the particles. As the spacing is narrowed, the electrical repulsive force generated between the plurality of particles having the same sign with each other increases, so that the spacing between the particles does not continue to be narrowed, but a predetermined balance is achieved. On the other hand, due to the electrical polarization characteristics of the particles, a plurality of particles are polarized in a predetermined direction, and the electrical attraction is locally generated between the plurality of polarized particles so that the spacing between the particles can be maintained at a predetermined interval. do. That is, according to the second embodiment of the present invention, the electrical attraction due to the external electric field, the electrical repulsive force between the particles having the same charge with each other and the electrical attraction due to the polarization of the plurality of particles at a distance (equilibrium) Can be arranged regularly. According to the above principle, the spacing between the particles can be controlled at a predetermined interval, and the plurality of particles arranged at a predetermined interval can function as a photonic crystal. Since the wavelength of light reflected from the plurality of particles arranged regularly is determined by the spacing between the particles, the wavelength of the light reflected from the plurality of particles can be arbitrarily controlled by controlling the spacing between the particles. Here, the pattern of the wavelength of the reflected light varies depending on factors such as the intensity and direction of the electric field, the size and mass of the particles, the refractive index of the particles and the solvent, the amount of charge of the particles, the electrical polarization characteristics of the particles, the concentration of dispersed particles in the solvent, and the like. May appear.

[Configuration of Light Transmitter Variable Material]

FIG. 6 is a diagram exemplarily illustrating a principle of adjusting light transmittance of a light transmittance variable material according to an exemplary embodiment of the present invention.

First, referring to FIG. 6A, when an electric field or a magnetic field is not applied, the plurality of particles 610 may be irregularly dispersed in the solvent 620, and the particles 610 and the solvent 620. The transmittance of the light incident on the) is not particularly controlled. That is, light incident on the variable light transmittance may be scattered or reflected by the plurality of particles 610 or the solvent 620 irregularly dispersed in the solvent 620 or may transmit the variable light transmittance as it is.

Next, referring to FIG. 6B, when an electric field is applied, the plurality of particles 610 may be polarized by an electric rheological phenomenon to rotate or move to be the same as the direction of the electric field. In addition, when a magnetic field is applied, each of the plurality of particles 610 may rotate or move so that the direction from the S pole to the N pole of the plurality of particles 610 is the same as the direction of the magnetic field. Further, according to an embodiment of the present invention, when the electric or magnetic field is applied, the plurality of particles 610 may be magnetized by the magnetic field and the plurality of particles 610 magnetized such that the magnetization direction is the same as the direction of the magnetic field. Each can rotate or move.

The anode and the cathode of each particle 610 rotated or moved in this way are the same as the cathode and the anode of the surrounding particle 610 or the N and S poles of each particle 610 are the S of the surrounding particles 610. As the poles and the north poles are close to each other, an electric or magnetic attraction occurs between the plurality of particles 610, so that the plurality of particles 610 regularly in a direction parallel to the direction of the electric or magnetic field. Can be aligned. That is, according to an embodiment of the present invention, as the electric or magnetic field is applied, the particles 610 in the light transmittance variable material may be aligned in a direction parallel to the direction of the electric or magnetic field, and thus the intensity or direction of the electric or magnetic field. By controlling the alignment of the particles to control the degree of light scattered or reflected by the particle 610 to the light transmittance variable material can be adjusted to further control the light transmittance.

7 and 8 are diagrams exemplarily illustrating a result of an experiment of applying a magnetic field in a state in which particles are dispersed in a solvent according to an embodiment of the present invention.

First, referring to FIG. 7A, when the magnetic field is applied in a direction perpendicular to the light transmittance variable material, the particles in the capsule are aligned in a direction parallel to the direction of the magnetic field (direction parallel to the direction of incident light). 7 (a), a plurality of particles aligned in a straight line are observed in the form of dots. Accordingly, the transmittance of light incident on the light transmittance variable material in a direction parallel to the direction of the magnetic field is relatively high. It can be seen that the increase.

Next, referring to FIG. 7B, when the magnetic field is applied to the variable light transmittance in a parallel direction or when the residual magnetization phenomenon appears in the particle even when the magnetic field applied to the variable light transmittance is blocked. It can be seen that the alignment is arranged in a direction perpendicular to the direction of the incident light while maintaining the alignment state of the straight form, and thus the transmittance of the light incident on the light transmittance variable material is relatively lowered.

Meanwhile, referring to FIG. 8A, when the magnetic field is not applied to the variable light transmittance, it may be confirmed that the transmittance of incident light uniformly appears in all regions of the variable light transmittance material without a large difference. Next, referring to FIG. 8B, a region in which a light transmittance variable material is generated with a magnetic field (that is, a magnetic field applied in a direction perpendicular to the light transmittance variable material) and a region where no magnetic field is generated are spaced a predetermined distance. In the case of placing on a magnet repeatedly arranged with respect to the magnetic field, the transmittance of light incident in a direction parallel to the direction of the magnetic field is high as the particles are aligned in a direction parallel to the direction of the magnetic field in a region where the magnetic field is applied. In the region where the magnetic field is not applied among the light transmittance variable materials, the particles are not aligned in the direction parallel to the direction of the magnetic field, so that they appear in the direction parallel to the direction of the magnetic field. It can be seen that the transmittance of the light to appear is low (that is, appear opaque).

For reference, in the experiment of FIG. 8, iron oxide particles (Fe ₂ O ₃ or Fe ₃ O ₄ ) having a size of 10 nm to 10 μm were used as the magnetic particles, and halogenated hydrocarbon oil was used as the solvent. A mixture of gelatin and acacia aqueous solution was used as capsule.

[Configuration of Target Material Detection Method and Apparatus Using Color-Variable Material or Light-Transmittance Variable Material]

9 is a diagram illustrating an operating principle of a detection device using a color variable material or a light transmittance variable material according to an embodiment of the present invention.

9, a detection apparatus according to an embodiment of the present invention may include a plurality of particles 910 having a detection reactor 920 formed on a surface thereof.

According to an embodiment of the present invention, when the target material 920 is not included in the test target material, the detection reactor 920 does not bind to the target material 930, thereby causing the hydrodynamic size of the particle 910 to be detected. characteristics such as hydrodynamic size, shape, surface charge, mass, etc. are not changed, and the particles 910 maintained as such are regularly spaced at first intervals (ie, inherent intervals) as electric or magnetic fields are applied. Are arranged to reflect light in the first wavelength range (i.e., inherent wavelength range) (Fig. 9 (a)).

In addition, according to an embodiment of the present invention, when the target material 930 is included in the test target material, the target material 930 is combined with the detection reactor 920, thereby providing a hydrodynamic size of the particle 910. (hydrodynamic size), shape, surface charge, and mass of at least one of the characteristics is changed, the particles 910 changed in this characteristic is corresponding to the second interval (ie, the target material 930 as the electric or magnetic field is applied) It is arranged regularly at a certain interval to reflect light of a second wavelength range (ie, a specific wavelength range corresponding to the target material 930) (FIG. 9B).

10 and 11 are diagrams exemplarily illustrating a configuration of a detection apparatus using a color variable material or a light transmittance variable material according to an embodiment of the present invention.

10 and 11, the detection apparatus 1000 or 1100 according to an embodiment of the present invention may include a solvent in which particles 1010 and 1110 in which a detection reactor is formed on a surface thereof are dispersed. It may include a test material injection holes 1030, 1130 for injecting a test material into the solvent, the magnet 1040, 1140 for applying a magnetic field to the particles or an electrode 1040 for applying an electric field , 1140). Meanwhile, the detection apparatus 1000 or 1100 according to an exemplary embodiment of the present invention further includes a control line in which the test substance is not injected separately from the test line into which the test target material is injected, thereby allowing the inspector to reflect the wavelength of the light reflected from the test line. The color (ie, color) can be compared with the wavelength of the light reflected from the control line to make it possible to more clearly determine whether the target material is detected.

First, as shown in FIG. 10, when the target material is included in the test target material, the target materials 1020 and 1120 are combined with the particles 1010 and 1110 having the detection reactor, thereby increasing the hydrodynamic size of the particles. When the magnetic field is applied to the particles whose properties are changed, the spacing between the particles becomes wider due to the size of the larger particles. Therefore, the wavelength of the light reflected from the particles of the sample line is longer than the wavelength of the light reflected from the particles of the control line, the inspector can observe the difference in the wavelength of the reflected light with the eye to determine that the target material is included in the material to be inspected. Will be.

Next, as shown in FIG. 11, if the target material is included in the test target material, the target materials 1020 and 1120 may be combined with the particles 1010 and 1110 having the detection reactor, so as to be on the surface of the negatively charged particles. Positive charges are formed, and when the magnetic field is applied to the particles whose properties are changed, the charges on the surface of the particles are changed to positive charges so that the particles move toward the upper electrode (that is, the (-) electrode) and are arranged regularly at a predetermined interval. do. Therefore, the wavelength of the light reflected from the particle of the sample line is different from the wavelength of the light reflected from the particle of the control line, and the inspector can visually observe the difference in the wavelength of the reflected light to determine whether the target material is included in the material to be inspected. Will be.

12 is a diagram illustrating a flowchart of a detection method using a color variable material or a light transmittance variable material according to an embodiment of the present invention.

Referring to FIG. 12, first, nanoparticles may be synthesized and coated with various surface materials to prepare a detection functional group-nanoparticle composite. Here, according to one embodiment of the present invention, the detection reactor may be conjugated to the surface of the nanoparticles using various linkers. Next, when the test substance is injected into the sample vessel containing the detection functional group-nanoparticle complex dispersed in a solvent, the detection target is antigen-antibody (antigen) into the detection reactor formed on the surface of the nanoparticle. -antibody) or electrostatic interactions, which can result in changes in at least one of the hydrodynamic size, shape, surface charge, and mass of the nanoparticles. Next, when an electric field or a magnetic field is applied to the sample line and the control line of the detection device, the nanoparticles are regularly arranged at predetermined intervals by electric or magnetic forces. The presence or absence of the target substance can be quickly and easily determined with reference to the color shift phenomenon generated thereby.

As described above, the present invention has been described by specific embodiments such as specific components and the like. For those skilled in the art, various modifications and variations are possible from these descriptions.

Accordingly, the spirit of the present invention should not be construed as being limited to the embodiments described, and all of the equivalents or equivalents of the claims, as well as the following claims, belong to the scope of the present invention .

110: particles
120: Solvent
130: Capsule
140: light transmissive film
610: particles
620: Solvent
910 particles
920: detection function
930: target substance
1000, 1100: detection device
1010, 1110: Particles with Detection Reactor
1020, 1120: target substance
1030, 1130: Test object injection hole
1040, 1140: magnet or electrode

Claims (9)

Mixing a material to be tested with a detection reactor capable of selectively binding with a target material to a solvent in which a plurality of particles formed on a surface are dispersed, and
Applying an electric field or a magnetic field to a solvent in which the test target material is mixed;
Including,
When the target material is included in the test target material, at least one of hydrodynamic size, shape, surface charge, and mass of the plurality of particles changes due to the target material binding to the detection reactor. and,
And a plurality of particles whose properties are changed are regularly arranged at intervals of a specific range as the electric or magnetic field is applied to reflect or transmit light having a specific wavelength range. The method of claim 1,
The target material detection method, characterized in that at least one of a protein, DNA, RNA, cells, bacteria and viruses. The method of claim 1,
The detection reactor, the target substance detection method characterized in that at least one of nickel (Ni), carboxyl group (-COOH), amine group (-NH ₂ ) and hydroxyl group (-OH). The method of claim 1,
The detection reactor, Protein A (Protein A), Protein G (Protein G), Streptavidin (Streptavidin), Anti-AFP and Anti-DEP at least one of the target substance detection method characterized in that it comprises. The method of claim 1,
And said particle comprises a superparamagnetic material. The method of claim 1,
The solvent detection method of the target material, characterized in that consisting of a visible light transmitting material. The method of claim 1,
The solvent is a target substance detection method, characterized in that it comprises at least one of a polymer of the network structure and a solid in the form of a gel (gel). The method of claim 1,
Determining whether the target material is included in the test target material by referring to a wavelength of light reflected from the solvent to which the electric or magnetic field is applied;
Target material detection method further comprises. A display unit including a plurality of particles formed on the surface of the detection reactor capable of selectively binding to the target material in a state of being regularly arranged, and into which the material to be inspected can be injected, and
Electromagnetic field generator for applying an electric or magnetic field to the display unit
Including,
When the target material is included in the test target material, at least one of the hydrodynamic size, shape, surface charge, and mass of the plurality of particles due to the target material is coupled to the detection reactor through the detection reactor. One characteristic changes,
And a plurality of particles whose properties are changed are regularly arranged at specific intervals as the electric or magnetic field is applied to reflect or transmit light in a specific wavelength range.

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