KR20130100036A - Camembert cheese and manufacturing method thereof - Google Patents

Camembert cheese and manufacturing method thereof Download PDF

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KR20130100036A
KR20130100036A KR1020120020299A KR20120020299A KR20130100036A KR 20130100036 A KR20130100036 A KR 20130100036A KR 1020120020299 A KR1020120020299 A KR 1020120020299A KR 20120020299 A KR20120020299 A KR 20120020299A KR 20130100036 A KR20130100036 A KR 20130100036A
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milk
cheese
lactis
lactococcus
goat
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KR1020120020299A
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KR101517871B1 (en
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정후길
최희영
김명진
김경희
이승구
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재단법인 임실치즈과학연구소
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00Cheese; Cheese preparations; Making thereof
    • A23C19/06Treating cheese curd after whey separation; Products obtained thereby
    • A23C19/068Particular types of cheese
    • A23C19/0682Mould-ripened or bacterial surface ripened cheeses
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00Cheese; Cheese preparations; Making thereof
    • A23C19/02Making cheese curd
    • A23C19/032Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A23C19/0325Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin using yeasts, alone or in combination with lactic acid bacteria or with fungi, without using other bacteria
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00Cheese; Cheese preparations; Making thereof
    • A23C19/02Making cheese curd
    • A23C19/05Treating milk before coagulation; Separating whey from curd
    • A23C19/054Treating milk before coagulation; Separating whey from curd using additives other than acidifying agents, NaCl, CaCl2, dairy products, proteins, fats, enzymes or microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/21Streptococcus, lactococcus
    • A23V2400/215Cremoris
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/21Streptococcus, lactococcus
    • A23V2400/231Lactis

Abstract

PURPOSE: A production method of camembert cheese is provided to produce the camembert cheese containing high contents of moisture, and having soft taste by the influence of protein decomposition, fat, flavor activation through containing surface fungi and starters. CONSTITUTION: Mixed milk is inoculated with a starter, and the mixture is fermented and cultured to obtain camembert cheese. The starter is selected from Lactococcus. lactis sub. lactis and Lactococcus. lactis sub. cremoris, or a mixture thereof instead of using a commercial strain. The starter is obtained by sterilizing reduced skimmed milk, inoculating Lactococcus. lactis sub. Lactis and Lactococcus. lactis sub. cremoris, and culturing the mixture. The mixed milk is raw milk, goat's milk, or their mixture. [Reference numerals] (AA) Step of preparing raw material; (BB) Step of pasteurizing at a low temperature; (CC) Step of cooling; (DD) Step of inoculating fermented cultured bacteria; (EE) Step of adding rennet and mold powder; (FF) Step of pulverizing; (GG) Step of mixing and draining; (HH) Step of injecting a mold; (II) Step of inoculating Penicillium.camemberti; (JJ) Step of adding salt and fermenting

Description

까망베르 치즈 및 그 제조방법{CAMEMBERT CHEESE AND MANUFACTURING METHOD THEREOF}Camembert cheese and its manufacturing method {CAMEMBERT CHEESE AND MANUFACTURING METHOD THEREOF}

본 발명은 까망베르 치즈 및 그 제조방법에 관한 것으로, 더욱 상세하게는 까망베르 치즈의 제조방법에 있어서, 1960년 당시 최초로 우리나라에서 사용되었던 까망베르 치즈 스타터로서 단독 균주인 mesophilic culture로 Lactococcus lactis subspecies diacetylactis Lactococcus lactis subspecies mesenteroides cremoris 를 사용하였다는 전문 서적 탐색으로 본 연구에서는 한국생명공학연구원 생물자원센터인 ACTC에서 분양받은 Lactococcus . lactis sub. lactis 와 국제생물자원센터인 ATCC에서 분양받은 Lactococcus . lactis sub. cremoris를 계대배양 후 사용하는 것을 특징으로 하는 까망베르 치즈의 제조방법에 관한 것이다.The present invention relates to Camembert cheese and a method for manufacturing the same, and more particularly, in the method of manufacturing Camembert cheese, Lactococcus as a mesophilic culture that is the only strain as a Camembert cheese starter that was used in Korea for the first time in 1960 lactis with subspecies diacetylactis Lactococcus lactis subspecies mesenteroides In this study, Lactococcus was obtained from ACTC, a biological resource center, Korea Research Institute of Bioscience and Biotechnology . lactis sub. lactis and Lactococcus distributed by ATCC, the International Center for Biological Resources . lactis sub. It relates to a method for producing Camembert cheese characterized in that the use of cremoris after subculture.

치즈는 수천 년 전부터 인간에게 알려진 발효식품이다. 현재 치즈의 종류는 1,400여 종 이상으로 알려져 있다. BC 6000년경 메소포타미아에 치즈와 비슷한 식품에 대한 기록이 있으므로 그 기원은 오래된 것으로 추측된다. 또한 BC 3000년경 스위스 코르테요 문화나 크레타섬의 미노아 문명 시대에 치즈 제조용인 목제기구의 출토가 있으므로 옛날부터 치즈가 이용된 것으로 생각된다.Cheese is a fermented food known to humans thousands of years ago. At present, there are more than 1,400 varieties of cheese. Since 6000 BC, there is a record of cheese-like food in Mesopotamia. It is also believed that cheese has been used since ancient times, since there was an excavation of wooden utensils for making cheese during the Swiss Corteyo culture and the Minoan civilization of Crete around 3000 BC.

1,400여 종류의 치즈가 있지만 서로 간에 비슷한 것들도 있고 아주 다른 것도 있는데 이는 형태, 크기, 숙성기간, 사용하는 원유, 첨가물, 포장법 그리고 제조지역에 따라 달라진다. 코덱스(Codex)에서는 계속해서 생산이 되는 치즈의 품질규격을 검토해 나가면서 개정해 나가고 있다.There are more than 1,400 types of cheese, but there are similar ones and very different ones, depending on the type, size, age, crude oil, additives, packaging, and manufacturing area. Codex continues to review and revise the quality standards of cheese produced.

치즈는 우유 중의 단백질과 지방이 거의 그대로 옮겨 간 것이라고 할 수 있는데, 커드가 형성될 때 단백질에 지방입자가 싸여서 같이 응고되어 침전된다. 따라서 치즈는 우유 중의 단백질과 지방이 약 1/10로 농축된 것이라고 볼 수 있다.Cheese can be said that the protein and fat in the milk almost transferred as it is, when the curd is formed, the fat particles are wrapped in the protein to coagulate and settle together. Therefore, cheese can be seen that the protein and fat in milk is about 1/10 of the concentration.

일반적으로 치즈에는 단백질과 지방이 20~30%씩 들어 있으며, 카티지나 크림치즈는 단백질보다 지방함량이 더 많다. 숙성이 된 치즈는 그 성분이 유산균 등의 효소의 영향으로 소화나 흡수가 되기 쉬운 형태로 변해 있다. 그 밖에 칼슘, 인 황 등 무기질과 비타민 A, B 등의 함유량도 많아 미용에도 좋으며, 성장하는 어린이는 물론 소화기관이 약한 환자나 노인들의 영양식품으로 적극 추천할만한 발효 식품이다.In general, cheese contains 20-30% of protein and fat. Cottage cheese and cream cheese contain more fat than protein. Aged cheese is changed into a form that is easily digested and absorbed by the effects of enzymes such as lactic acid bacteria. In addition, minerals such as calcium and phosphorus, and vitamin A, B and other contents are also good for beauty, it is a fermented food that is highly recommended as a nutritional food for the growing children as well as patients with weak digestive organs or the elderly.

과거 우리나라에서 치즈가 처음 생산된 것은 1968년 전북 임실의 지정환 신부가 산양유를 이용해 프랑스의 까망베르 치즈 생산을 시도한 것이 효시로 1968년에는 '성가' 치즈가 제품화되어 1969년 체다 치즈 계열인 '정환'치즈가 제품화되어 조선호텔에 납품된 바도 있다. 이후 1970년대 초에 서울우유(협)에서 체다 형태의 자연치즈를 생산하고 1974년 5월 삼양식품에서 가공치즈를 만들기 시작하였다.In the past, cheese was first produced in Korea in 1968 when Jeon Jung-hwan, a priest of Imsil, Jeollabuk-do, tried to produce Camembert cheese in France using goat's milk. In 1968, 'sung' cheese was commercialized. Was commercialized and delivered to Chosun Hotel. Later, in the early 1970s, Seoul Milk (Hyup) produced cheddar-type natural cheese, and in May 1974, it started making processed cheese from Samyang Food.

까망베르 치즈(Camembert cheese)는 프랑스 노르만의 까망베르 마을이 원산지이며 수분함량은 40% 이상이고 F.I.D.M.(Fat In Dry Matter) 함량은 45%정도이며 125g~500g정도 무게를 지닌 원반형으로 흰색의 표면 곰팡이에 의해 3주일 정도 숙성시킨 특유의 풍미가 있고 단백질이 분해된 연질치즈로 치즈의 여왕이라고 알려졌을 정도로 유명한 치즈이다. 일반적으로 사용되는 표면 곰팡이 균주는 Penicillium camemberti, Penicillium caseicolum, Penicillium candidium 등이며 스타터에 사용되는 유산균주는 현재 Chr. Hansen사의 상업적 균주인 Flora Danica (Lactococcus lactis ssp. Lactis, Lactococcus lactis ssp. cremoris, Lactococcus lactis ssp. lactis biovar . diacetylactis, Leuconostoc mesenteroides ssp. cremoris)와 같은 복합균주를 사용한다.The camembert cheese is native to the Camembert village of Norman, France, and has a disc content of over 40%, FIDM (Fat In Dry Matter) content of 45%, and weighs 125g ~ 500g. It has a distinctive flavor that has been aged for about three weeks and is known for being the cheese queen. Commonly used surface fungal strains are Penicillium camemberti, Penicillium caseicolum, Penicillium candidium, etc. The lactic acid strains used for starters are currently Chr. Hansen's commercial strain Flora Danica (Lactococcus lactis ssp. Lactis , Lactococcus lactis ssp. Cremoris, Lactococcus lactis ssp. Lactis biovar . diacetylactis , Leuconostoc mesenteroides ssp. use complex strains such as cremoris ).

국내 및 외국에서는 대부분 Cheddar 또는 Gouda 치즈와 같은 경질치즈의 숙성중 풍미, 단백질, 지방, 조직등의 변화에 관한 연구가 많이 이루어져 왔으며 까망베르 치즈와 같은 연질치즈에 대한 연구는 많이 이루어지지 않았다.Most domestic and foreign studies have been conducted on the changes of flavor, protein, fat, and tissue during ripening of hard cheeses such as Cheddar or Gouda cheese, and little studies on soft cheeses such as Camembert cheese.

연질 치즈의 일종인 까망베르 치즈는 높은 수분을 함유하고 있고, 표면곰팡이와 스타터의 공존으로 단백질 분해, 지방, 풍미 등의 활성화의 영향으로 부드러운 맛을 가지고 있어 우리 입맛에 맞는 치즈의 한 종류이다. 까망베르 치즈는 빠른 산 진행 즉, 숙성기간이 빠르고 보존기간이 짧아 수입 자유화 이후에도 국제적인 경쟁력이 있다고 사료된다.Camembert cheese, a kind of soft cheese, contains high moisture and has a soft taste due to the coexistence of surface fungus and starter, and has a soft taste due to the activation of protein breakdown, fat, and flavor. Camembert cheese is considered to be internationally competitive even after import liberalization because of its rapid acid progress, that is, its ripening period is short and its preservation period is short.

우리나라의 유산양 사육은 1903년 saanen종의 도입으로 시작되었으나, 실제로는 1960년대 초부터 본격적으로 소개되었다고 볼 수 있다. 산양은 조사료 위주로 사육이 가능할 뿐 아니라 분뇨에 의한 환경오염이 적고 순환농업에 적합한 가축의 하나로 알려지고 있으며 주로 Saanen종이 주류를 이루고 있으며 세계적으로 소비되는 유식품의 상당량은 산양유나 산양유제품이 차지하고 있을 만큼 중요하며, 최근에는 산양유의 기능성이 밝혀지면서 산양유 산업은 앞으로 전망이 밝다고 한다. 일반적으로 산양은 반추가축 중에서도 조사료의 이용성이 뛰어나고 특히 섬유소의 소화율이 상대적으로 높은 가축으로 알려져 있다.The breeding of abortions in Korea began with the introduction of saanen in 1903, but in fact it was introduced in earnest from the early 1960s. Goats are not only farmable, but are known to be one of the most suitable livestock farming farms with low environmental pollution due to manure. Saanen species are the mainstream, and most of the dairy foods consumed worldwide are goat or goat dairy products. Importantly, as the function of goat's milk is revealed recently, the goat's milk industry has a bright future. In general, goats are known to have high availability of forage among ruminants and particularly high digestibility of fiber.

본 발명은 높은 수분을 함유하고 있고, 표면곰팡이와 스타터의 공존으로 단백질 분해, 지방, 풍미 등의 활성화의 영향으로 부드러운 맛을 내는 까망베르 치즈를 우리나라에서 최초로 제조된 방법으로 지역적 고부가가치의 기능성 치즈를 제공하는데 그 목적이 있다.The present invention is a method of producing a high-quality functional cheese of Camembert cheese, which contains high moisture and has a soft taste due to the coexistence of surface fungus and starter, which is affected by protein degradation, fat and flavor activation. The purpose is to provide.

상기와 같은 목적을 달성하기 위하여, 본 발명에 따른 까망베르 치즈의 제조방법은 최초로 사용되었던 원유인 산양유와 현재 사용량이 많은 원유, 원유와 산양유를 혼합한 믹스유를 이용하여 까망베르 치즈를 제조하는 방법에 있어서, 상기 치즈 제조방법에서 사용하는 스타터는 Lactococcus . lactis sub. lactisLactococcus . lactis sub. cremoris 중 동시에 사용하는 것과 기본적인 치즈의 방법을 위해 원유에 상업적 스타터인 Flora Danica 복합균주를 첨가한 제조방법을 특징으로 한다.In order to achieve the above object, the manufacturing method of Camembert cheese according to the present invention is a method for producing Camembert cheese using a mixed oil of the first used goat oil and crude oil, crude oil and goat's milk is currently used In, the starter used in the cheese manufacturing method is Lactococcus . lactis sub. lactis and Lactococcus . lactis sub. It is characterized by the addition of a commercial starter Flora Danica complex to crude oil for simultaneous use of cremoris and for the method of basic cheese.

또한, 상기 스타터는 환원탈지유를 살균하여 Lactococcus. lactis sub. lactisLactococcus. lactis sub. cremoris를 접종한 후 배양하여 제조되는 것을 특징으로 한다.In addition, the starter sterilized reduced skim milk Lactococcus. lactis sub. lactis and Lactococcus. lactis sub. After inoculating cremoris is characterized in that it is prepared by culturing.

또한, 본 발명에 따른 까망베르 치즈는 산양유 또는 원유와 산양유가 혼합된 믹스유에 스타터로서 Lactococcus . lactis sub. lactisLactococcus . lactis sub. cremoris 중 동시에 접종하여 발효배양되어 제조된 것을 특징으로 한다.In addition, the Camembert cheese according to the present invention is Lactococcus . lactis sub. lactis and Lactococcus . lactis sub. cremoris It is characterized in that the fermentation culture was prepared by inoculating at the same time.

본 발명에 따르면, 까망베르 치즈에 원유, 산양유 또는 원유와 산양유를 혼합한 믹스유를 첨가하여 영양과 맛이 우수한 치즈를 제공할 수 있다.According to the present invention, cheese, which has excellent nutrition and taste, can be added to Camembert cheese by adding crude oil, goat's milk or mixed oil mixed with crude and goat's milk.

또한, 높은 수분을 함유하고 있고, 표면곰팡이와 스타터의 공존으로 단백질 분해, 지방, 풍미 등의 활성화의 영향으로 부드러운 맛을 내는 고부가가치 치즈로서의 이용가능성을 확인한 효과가 있다.In addition, it has the effect of confirming the availability as a high value-added cheese that contains high moisture and has a soft taste due to the coexistence of the surface mold and the starter and the activation of protein breakdown, fat and flavor.

도 1은 까망베르 치즈의 제조방법.
도 2는 까망베르 치즈의 숙성과정 중 유산균수의 변화를 나타낸 그래프.
도 3은 까망베르 치즈 숙성 기간 중 4일 간격으로 실험한 pH의 변화를 나타낸 그래프.
도 4는 산양유의 함량별 까망베르 치즈의 숙성 후 색, 향미, 조직감, 전체적인 선호도에 대한 관능평가 그래프.
1 is a method of manufacturing Camembert cheese.
Figure 2 is a graph showing the change in the number of lactic acid bacteria during the ripening process of Camembert cheese.
Figure 3 is a graph showing the change in pH tested at intervals of 4 days during the Camembert cheese ripening period.
Figure 4 is a sensory evaluation graph for the color, flavor, texture, overall preference of aging of Camembert cheese by the amount of goat milk.

본 발명은 까망베르 치즈의 제조방법에 있어서, 원유, 산양유 또는 원유와 산양유를 혼합한 믹스유를 이용하여 까망베르 치즈를 제조하는 방법에 있어서, 상기 치즈 제조방법에서 사용하는 스타터로는 원유에 상업적 균주인 Flora Danica 복합균주와 한국생명공학연구원 생물자원센터 및 국제생물자원센터에서 분양 받은 Lactococcus. lactis sub. lactisLactococcus . lactis sub. cremoris 를 동시에 사용한다.The present invention is a method for producing Camembert cheese, raw milk, goat's milk or a method for producing Camembert cheese using a mixture of milk and goat's milk mixed, the starter used in the cheese manufacturing method is a commercial strain in crude oil Lactococcus obtained from Flora Danica complex and from Korea Institute of Bioscience and Biotechnology Center and International Center for Biological Resources . lactis sub. lactis and Lactococcus . lactis sub. Use cremoris at the same time.

또한, 본 발명에서 까망베르 치즈는 원유, 산양유 또는 원유와 산양유를 혼합한 믹스유 중 어느 하나를 사용한다.In addition, the Camembert cheese in the present invention uses any one of crude oil, goat milk or mixed oil mixed with crude and goat milk.

종래의 까망베르 치즈의 제조에서 일반적으로 스타터에 사용되는 유산균주는 Chr. Hansen사의 상업적 균주인 Flora Danica (Lactococcus lactis ssp. Lactis, Lactococcus lactis ssp. cremoris, Lactococcus lactis ssp. lactis biovar. diacetylactis, Leuconostoc mesenteroides ssp. cremoris)와 같은 복합균주를 사용하였는데, 본 발명에서는 산양유와 믹스유에 치즈제조로 사용된 스타터로서 한국생명공학연구원 생물자원센터인 ACTC에서 분양받은 Lactococcus . lactis sub. lactis 와 국제생물자원센터인 ATCC에서 분양받은 Lactococcus . lactis sub. cremoris를 계대배양 후 사용하는 것을 특징으로 한다. In the production of conventional Camembert cheese, lactic acid bacteria which are generally used in starters are Chr. Hansen's commercial strain Flora Danica ( Lactococcus) lactis ssp. Lactis , Lactococcus lactis ssp. cremoris , Lactococcus lactis ssp. lactis biovar. diacetylactis , Leuconostoc mesenteroides ssp. cremoris ) was used, but in the present invention, Lactococcus . lactis sub. lactis and Lactococcus distributed by ATCC, the International Center for Biological Resources . lactis sub. It is characterized by the use of cremoris after subculture.

산양유 또는 믹스유의 경우에는 산양의 고유한 향과 원유에 비하여 유성분 함량이 높은 때문에 많은 치즈제조 특히, 까망베르 치즈를 제조하기가 어려웠는바, 본 발명에서는 이러한 문제점을 해결하기 위하여, 산양의 고유의 향을 제거하고 P/F의 비율을 일정하게 하여 Lactococcus . lactis sub. lactisLactococcus . lactis sub. cremoris를 까망베르 치즈의 제조과정에 사용하여 제조하였다.In the case of goat milk or mixed oil, it is difficult to manufacture many cheeses, especially Camembert cheese, because the milk content is higher than goat's inherent aroma and raw milk. Lactococcus by removing and keeping the P / F ratio constant . lactis sub. lactis and Lactococcus . lactis sub. cremoris was used in the manufacture of Camembert cheese.

실시예Example

1. 원유Crude oil

전북 임실군 금성리에 소재 푸른목장(대표: 이기채)에서 사육중인 홀스타인 프리지안(Holstein-Friesian)종의 당일 착유한 신선한 원유를 사용하였다(TA:0.14~0.15%, pH: 6.8).Fresh crude milk milked on the day of Holstein-Friesian species, bred in the blue ranch, Geumseong-ri, Imsil-gun, Jeonbuk, Korea (TA: 0.14 ~ 0.15%, pH: 6.8).

2. 산양유2. Goat Milk

전북 임실군 금성리에 소재 두마리목장(대표: 심요섭)에서 사육중인 산양유(Goat Milk)의 당일 착유한 신선한 산양유를 사용하였다(TA: 0.20%, pH:6.8)Fresh goat milk milked on the day of Goat Milk, which is being raised at two horse farms in Geumseong-ri, Imsil-gun, Jeonbuk, Korea (TA: 0.20%, pH: 6.8)

3. 치즈 스타터(starter)3. Cheese Starter

치즈 스타터(starter)는 원유에는 Chr. Hansen사의 상업적 균주인 Flora Danica (Lactococcus lactis ssp. Lactis, Lactococcus lactis ssp. cremoris, Lactococcus lactis ssp. lactis biovar. diacetylactis, Leuconostoc mesenteroides ssp. cremoris)를 DVS(Direct vat Starter)로 사용하고 산양유와 믹스유는 단독 균주(Lactococcus. lactis sub. lactis 와 Lactococcus. lactis sub. cremoris)를 고형분 농도 10%의 멸균 탈지유에 2회 계대 배양하여 활력을 증진시킨 후 원유의 1.5%양을 제조, 사용하였다. 즉 환원탈지유를 95에서 30분간 살균하여 산양유와 믹스유에는 Lactococcus. lactis sub. lactisLactococcus . lactis sub. cremoris를 각각 접종한 후, 22 ~ 25에서 24시간 ~ 48 동안 인큐베이터에서 배양하여 사용하였다.The cheese starter is Crr. Hansen's commercial strain Flora Danica ( Lactococcus) lactis ssp. Lactis , Lactococcus lactis ssp. cremoris , Lactococcus lactis ssp. lactis biovar. diacetylactis , Leuconostoc mesenteroides ssp. cremoris ) is used as a direct vat starter (DVS), and goat milk and mixed oils are used as a single strain (Lactococcus. After enhancement, 1.5% of crude oil was produced and used. In other words, sterilized reduced skimmed milk for 95 to 30 minutes, and Lactococcus. lactis sub. lactis and Lactococcus . lactis sub. After inoculating cremoris , the cultures were incubated at 22-25 for 24 hours to 48 hours.

4. 치즈의 품질 분석4. Quality Analysis of Cheese

(1) 유산균 수 측정(1) lactic acid bacteria count

치즈 숙성 중 유산균 수 변화는 4일마다 경시적으로 검사하였다. 원유(Raw milk), 산양유(Goat milk), 원유와 산양유 믹스유(Mixing milk) 의 함량에 따른 까망베르 치즈 시료는 생리식염수(saline)와 치즈 시료를 2:1의 비율로 분쇄용 튜브에 넣어 균질기 (HG-15D, WiseTis, Korea)를 사용하여 2.0 이하에서 최대속도인 20,000 rpm으로 2분간 균질을 3차례 반복하여 Richardson(1983)의 방법에 따라 10진 희석 후 MRS 배지를 이용하여 standard plate count법으로 37에서 48시간 배양 후 colony 수가 30~300개 범위로 나타난 평판을 선별하여 계측에 사용하였다.Changes in the number of lactic acid bacteria during cheese ripening were examined over time every four days. Camembert cheese samples according to the contents of raw milk, goat milk, mixed milk and mixing milk (mixing milk) are homogenized by putting saline and cheese samples in a ratio of 2: 1 in a grinding tube. Repeat the homogenization three times for 2 minutes at 20,000 rpm, the maximum speed of 2.0 or less at the maximum speed of 2.0 or less using HG-15D, WiseTis, Korea. As a method, after incubation at 37 to 48 hours, the colonies were screened for 30 to 300 colonies.

(2) pH 측정 (2) pH measurement

원유(Raw milk), 믹스유(Mixing milk), 산양유(Goat milk)의 함량에 따른 까망베르 치즈 숙성 중 pH는 생균수 측정법과 동일한 방법으로 멸균 Saline과 치즈를 2:1의 비율 (saline:cheese=20㎖:10g)로 분쇄용 tube에 넣어 균질기 (HG-15D, WiseTis®, Korea)로 최대속도 20,000 rpm으로 2분간 균질한 다음 pH meter (UB-10 Delux, Denver, USA)를 사용하여 4일 간격으로 16일 동안 측정하였다.During fermentation of Camembert cheese according to the content of Raw milk, Mixing milk and Goat milk, the pH is 2: 1 ratio of sterile Saline and cheese (saline: cheese = 20ml: 10g) into a grinding tube and homogenized with a homogenizer (HG-15D, WiseTis®, Korea) at a maximum speed of 20,000 rpm for 2 minutes and then using a pH meter (UB-10 Delux, Denver, USA). Measurements were made for 16 days at daily intervals.

(3) 일반성분 측정(3) General composition measurement

원유(Raw milk), 믹스유(Mixing milk), 산양유(Goat milk)의 함량에 따른 까망베르 치즈의 일반성분은 마지막 16일차 치즈를 FoodScanTM(FoodScan dairy analyzer, Denmark)을 이용하여 지방, 단백질, 수분함량, 염도, 총고형량(Total Solid)를 측정하였다.The general ingredients of Camembert cheese according to the content of Raw milk, Mixing milk and Goat milk are the last 16 days of the cheese, using FoodScan TM (FoodScan dairy analyzer, Denmark) for fat, protein and moisture. The content, salinity and total solids were measured.

(4) 까망베르 치즈의 제조방법(4) manufacturing method of Camembert cheese

본 발명에서 사용한 까망베르 치즈의 제조과정은 도 1에 도시되어 있다.The manufacturing process of Camembert cheese used in the present invention is shown in FIG.

먼저, 원유(Raw milk), 원유와 산양유가 50:50으로 혼합된 믹스유(Mixing with Goat milk), 산양유(Goat milk)(T.A 0.16%, pH6.8)를 각각 63℃에서 30분간 저온살균하였다. 이후, 32℃에서 식히고(Cooling), 치즈를 제조하기 위해 탱크에 넣었다(Setting in Vat).First, raw milk (Mixing with Goat milk) and goat milk (Goat milk) (TA 0.16%, pH6.8) mixed at 50:50 are pasteurized at 63 ° C. for 30 minutes. It was. Cooling was then carried out at 32 DEG C, and setting in vat was made to produce cheese.

살균한 후 냉각한 원유와, 믹스유와, 산양유 100중량부에 대하여 스타터 1중량부를 접종하고, 원유와, 믹스유와, 산양유 100l에 대하여 렌넷(Rennet) 16과, 곰팡이파우더(Mold powder)4를 더 첨가하였다.After sterilization, 1 part by weight of the starter is inoculated with 100 parts by weight of the cooled crude oil, mixed oil, and goat milk.Rennet 16 and mold powder4 are applied to 100 liters of the crude oil, mixed oil and goat milk. More was added.

이후, 치즈 나이프로 분쇄하고 5분 동안 기다렸다.Thereafter, it was ground with a cheese knife and waited for 5 minutes.

이후, 32℃에서 30분간 휘저은 다음 유청을 배수했다.After stirring at 32 ° C. for 30 minutes, the whey was then drained.

다음으로, 유청을 배수한 커드를 몰드에 투입하고, Penicillium . camemberti 균을 접종한다.Next, the whey drained curd is poured into a mold, and the Penicillium . Inoculate the camemberti bacteria.

이후, 15℃에서 20%의 염도를 갖도록 가염한 후(Salting), 10℃에서 숙성시켰다(Ripening).Then, after salting to have a salinity of 20% at 15 ° C (Salting), and aged at 10 ° C (Ripening).

(5) 관능평가(5) Sensory evaluation

관능검사는 임실치즈과학연구소 연구원 10명을 대상으로 하여 9점 척도법을 이용하여 동일 설문지로 평가하였다. 이때 관능검사의 평가 항목은 색감(color), 향미(flavor), 맛(Taste), 조직감(Texture) 및 종합적인 기호도(overall acceptability)로 나타내었고 평가내용으로는 대단히 좋아한다: 9점, 좋지도 싫지도 않다: 5점, 대단히 싫어한다: 1점으로 나타내었고, 시료의 준비 및 제시는 50ml의 컵에 샘플번호를 붙여서 제공하였다. 선별된 패널은 나이, 성별 등을 기록하였고 각 시료는 물컵, 시료를 뱉는 컵과 정수기에서 받은 물을 시료 사이에 제공하였다. The sensory evaluation was conducted on 10 researchers at the Imsil Cheese Research Institute using the 9-point scale method. The evaluation items of sensory evaluation were expressed in color, flavor, taste, texture, and overall acceptability. Not hate: 5 points, hate very much: 1 point, the preparation and presentation of the samples were provided with a sample number in a 50 ml cup. Selected panels recorded age, gender, etc. Each sample provided a water cup, a spitting cup, and water received from a water purifier between the samples.

Ⅲ. 결과 및 고찰Ⅲ. Results and Discussion

1. 유산균 수 변화1. Change in Lactic Acid Bacteria

도 2에는 Richardson(1985) 방법에 의하여 원유, 믹스유, 산양유의 함량을 달리한 까망베르 치즈의 숙성과정 중 유산균수의 변화를 조사한 결과가 도시되어 있다.Figure 2 shows the results of examining the change in the number of lactic acid bacteria during the ripening process of Camembert cheese with different amounts of crude, mixed and goat's milk by Richardson (1985) method.

여기에서, ◆-◆는 원유(Raw milk), ■-■ 는 믹스유(Mixing milk), ▲-▲는 산양유(Goat milk)이다.Here, ◆-◆ is Raw milk, ■-■ is Mixing milk, ▲-▲ is Goat milk.

연구결과 함량에 따라 유산균 수의 변화에는 원유의 함량과 종류에 따라 큰 차이는 없었으며 산양유의 함량이 유산균 증식 억제 작용을 나타내지 않으며 치즈의 숙성을 완만하게 하는 결과를 나타내었다.The results showed that there was no significant difference in the number of lactobacillus according to the content, and the amount of goat's milk did not inhibit the growth of lactic acid bacteria and resulted in the aging of cheese.

2. pH 변화2. pH change

도 3에는 16일간의 까망베르 치즈 숙성 기간 중 4일 간격으로 실험한 pH의 변화가 도시되어 있다.Figure 3 shows the change in pH tested at 4 day intervals during the 16-day Camembert cheese ripening period.

여기에서, ◆-◆는 원유(Raw milk), ■-■ 는 믹스유(Mixing milk), ▲-▲는 산양유(Goat milk)이다.Here, ◆-◆ is Raw milk, ■-■ is Mixing milk, ▲-▲ is Goat milk.

까망베르 치즈 압착 시 평균 치즈의 pH보다 다소 낮은 값을 보였는데 이는 Penicillium camemberti라는 까망베르 치즈에 사용되는 곰팡이균에 의해 발효과정 중 pH가 높아지는 것을 보여준다. pH가 4.6~4.9로 낮은 pH로 시작하여 숙성이 진행되어 곰팡이가 자라나는 15일 이후에 최종 pH는 5.2~5.3까지 상승하여 까망베르 치즈의 본래의 pH를 유지하였다. When pressing the camembert cheese, the pH of the cheese was slightly lower than that of the average cheese, indicating that the fungus used in the Camembert cheese, Penicillium camemberti, increases the pH during fermentation. After the low pH of 4.6-4.9 and ripening progressed, the final pH was increased to 5.2-5.3 after 15 days of mold growth to maintain the original pH of Camembert cheese.

3. 일반성분 변화3. Changes in General Ingredients

단백질protein 지방Fat 수분함량Moisture content 염도Salinity TSTS 원유crude oil 15.5615.56 21.8721.87 54.0154.01 1.461.46 45.9945.99 믹스유Mix oil 15.6615.66 22.0822.08 54.1754.17 1.361.36 45.8345.83 산양유Goat milk 15.7415.74 22.1222.12 54.2254.22 1.381.38 45.7845.78

함량에 따라 제조된 까망베르 치즈의 일반성분을 분석한 결과는 표 1에서 보는 바와 같이 원유와 믹스유, 산양유로 제조된 까망베르 치즈의 일반성분은 유의적 차이가 나지 않은 것으로 나타났다. As a result of analyzing the general ingredients of Camembert cheese prepared according to the content, it was found that the general ingredients of Camembert cheese prepared with crude oil, mixed oil and goat milk did not differ significantly.

4. 관능평가4. Sensory Evaluation

도 4는 산양유의 함량별 까망베르 치즈의 숙성 후 색, 향미, 조직감, 전체적인 선호도가 도시되어 있다.Figure 4 shows the color, flavor, texture, and overall preference of aging Camembert cheese by the amount of goat's milk.

여기에서, ◆-◆는 원유(Raw milk), ■-■ 는 믹스유(Mixing milk), ▲-▲는 산양유(Goat milk)이다.Here, ◆-◆ is Raw milk, ■-■ is Mixing milk, ▲-▲ is Goat milk.

색, 전체적인 선호도에서는 큰 차이를 보이지 않았으나 색을 제외한 다른 부분에서 원유를 이용한 까망베르 치즈보다 산양유를 이용한 까망베르 치즈에서 다소 낮은 값을 보였지만 유의적 차이는 나타나지 않았다.There was no significant difference in color and overall preference, but it was slightly lower in Camembert cheese using goat milk than in Camembert cheese using crude oil except for color.

추후 산양유 특유의 냄새를 제거하여 까망베르 치즈를 제조하는 기술에 대한 연구가 필요할 것으로 사료된다.Further research on the technology of preparing Camembert cheese by removing the unique smell of goat's milk is needed.

Ⅳ. 요 약IV. summary

본 연구는 임실치즈의 고부가가치 산업화를 조성하여 임실 신성장동력 산업인 치즈 산업의 경쟁력을 강화를 통하여 임실 치즈의 원조급인 전통적 까망베르 치즈인 산양유 까망베르 치즈를 제조하여 지역 및 기업의 역량강화, 임실치즈 제품의 다변화를 통한 신규 시장을 개척하기 위함을 목적으로 임실지역에서 최조로 제조된 까망베르 치즈를 산양유와 원유의 함량을 달리하여 제조된 까망베르 치즈의 품질에 미치는 영향을 조사하였다. 원유, 원유와 산양유를 50:50 비율로 혼합한 믹스유, 산양유 3가지 함량으로 첨가하여 제조함으로써 까망베르 치즈의 숙성 과정 중 미생물이 미치는 영향과 산 생산성 변화를 알아보았다. 숙성 종료 후 치즈의 일반성분과 관능검사를 실시하여 임실 전통성 복원 치즈 개발을 위한 기초자료를 활용하고자 수행한 본 연구에서는 다음과 같은 결과를 확인하였다.This study is to create high value-added industrialization of Imsil cheese and strengthen the competitiveness of the cheese industry, a new growth engine industry, to produce goat's milk camembert cheese, a traditional camembert cheese that is the aid of Imsil cheese. The purpose of this study was to investigate the effects of Camembert cheese, which was prepared most recently in Imsil, on the quality of Camembert cheese produced by varying the amount of goat milk and crude oil. Raw milk, mixed milk and goat's milk in 50:50 ratio were added and prepared in three contents to examine the effects of microorganisms and acid productivity during the ripening process of Camembert cheese. After completion of fermentation, this study was conducted to utilize the basic data for the development of imperative traditional restoration cheese by conducting the general composition and sensory test of cheese.

1. 원유, 믹스유, 산양유 까망베르 치즈의 숙성 중 미생물의 증식과 산 생산성에 있어 각각 다른 원유로 제조된 까망베르 치즈는 미생물증식과 산 생산성에는 큰 영향을 미치지 않았다.1. Raw milk, mix oil, and goat's milk Camembert cheeses made with different crude oils for the growth and acid productivity of the camembert cheese had no significant effect on the microbial growth and acid productivity.

2. 숙성 후 일반성분 분석에서는 함량에 따른 변화에는 큰 영향을 미치지 않았다.2. In the general component analysis after aging, there was no significant effect on the change according to the content.

3. 관능검사 결과 큰 차이는 발견되지 않았으나 향미에 있어서 산양유 특유의 유취로 인한 거부 반응이 약간 발생하는 것을 볼 수 있었으나 이는 추후 산양유의 유취를 제거할 수 있는 기술력을 이용한 까망베르 치즈 제조의 기술적 연구가 필요할 것으로 사료되었다.3. As a result of sensory evaluation, no significant difference was found, but there was a slight rejection of goat milk due to the smell of goat's milk. However, the technical research on the manufacture of Camembert cheese using technology that can remove the goat's milk smell later It was considered necessary.

이상의 결과를 종합하여 보면 다른 원유와 함량을 달리하여 제조된 까망베르 치즈는 품질변화에 큰 영향을 미치지 않았고 관능적인 면에 있어서 고유의 유취를 제거한 치즈 개발을 통한 제조방법에 따라 임실의 전통성을 복원하는 산양유 까망베르 치즈 개발이 기대되었다.Taken together, the Camembert cheese produced with different contents from other crude oils did not have a significant effect on the quality change and restored the traditionality of Imsil according to the manufacturing method through the development of cheese which eliminated the inherent odor in the sensual aspect. Goat milk camembert cheese development was expected.

Claims (3)

원유, 산양유 또는 원유와 산양유를 혼합한 믹스유를 이용하여 까망베르 치즈를 제조하는 방법에 있어서,
상기 치즈 제조방법에서 사용하는 스타터는 상업적 균주의 선택이 아닌 Lactococcus. lactis sub. lactisLactococcus. lactis sub. cremoris 중 하나를 선택하거나 동시에 사용하는 것을 특징으로 하는 까망베르 치즈의 제조방법.
In the method for producing Camembert cheese using crude oil, goat milk or mixed oil mixed with raw milk and goat milk,
The starter used in the cheese manufacturing method is Lactococcus. lactis sub. lactis and Lactococcus. lactis sub. cremoris Method for producing Camembert cheese, characterized in that the use of one or selected at the same time.
제1항에 있어서, 상기 스타터는 환원탈지유를 살균하여 Lactococcus . lactis sub. lactis Lactococcus. lactis sub. cremoris를 접종한 후 배양하여 제조되는 것을 특징으로 하는 까망베르 치즈의 제조방법.The method of claim 1, wherein the starter is sterilized reduced skim milk Lactococcus . lactis sub. lactis And Lactococcus. lactis sub. Method of producing Camembert cheese, characterized in that it is produced by inoculating cremoris after inoculation. 원유, 산양유 또는 원유와 산양유가 혼합된 믹스유에 스타터로서 Lactococcus. lactis sub. lactisLactococcus . lactis sub. cremoris 중 하나를 선택하거나 동시에 접종하여 발효배양되어 제조된 것을 특징으로 하는 까망베르 치즈. Lactococcus as a starter in crude, goat's milk or a mix of crude and goat's milk . lactis sub. lactis and Lactococcus . lactis sub. cremoris Camembert cheese, characterized in that produced by fermentation culture by selecting one or at the same time inoculated.
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