KR20130077153A - Rice beverage composition containing functional sugar and the method thereof - Google Patents

Rice beverage composition containing functional sugar and the method thereof Download PDF

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KR20130077153A
KR20130077153A KR1020110145712A KR20110145712A KR20130077153A KR 20130077153 A KR20130077153 A KR 20130077153A KR 1020110145712 A KR1020110145712 A KR 1020110145712A KR 20110145712 A KR20110145712 A KR 20110145712A KR 20130077153 A KR20130077153 A KR 20130077153A
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rice
weight
lactobacillus
bifidobacterium
enzyme
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KR101323479B1 (en
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김정훈
김미정
이진희
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웅진식품주식회사
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/152Milk preparations; Milk powder or milk powder preparations containing additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/72Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/065Microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/02Acid
    • A23V2250/032Citric acid
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/20Natural extracts
    • A23V2250/21Plant extracts
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/60Sugars, e.g. mono-, di-, tri-, tetra-saccharides
    • A23V2250/62Palatinose, isomaltulose
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00Processes
    • A23V2300/24Heat, thermal treatment
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00Processes
    • A23V2300/50Concentrating, enriching or enhancing in functional factors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225Lactobacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225Lactobacillus
    • C12R2001/25Lactobacillus plantarum

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Microbiology (AREA)
  • Non-Alcoholic Beverages (AREA)

Abstract

PURPOSE: A rice beverage composition is provided to improve the unique flavor and taste of rice by fermenting the rice, to offer excellent palatability and functionality, and to contain lactobacillus for a long time. CONSTITUTION: Roasted brown rice and white rice are pulverized, and are mixed in a weight ratio of 1-2:9-8. 4-8 times the amount of purified water is added to the mixed ingredients, and the mixed ingredients are heated at 100 deg. C while stirring. Stirred ingredients are cooled to 88-96 deg. C, and 0.02-0.06 parts of bacteria alpha-amylase by weight per 100 parts of ingredients by weight is inserted to obtain first enzyme decomposition liquid. 0.05-0.25 parts of glucoamylase by weight, 0.1-0.3 parts of protease by weight, and 0.05-0.25 parts of pectinase are inserted into 100 parts of first enzyme decomposition liquid by weight to obtain second enzyme decomposition liquid. The second enzyme decomposition liquid is deactivated, and filtered to obtain rice concentrate.

Description

기능성 당을 함유하는 쌀 음료 조성물 및 그 제조방법{Rice Beverage Composition Containing Functional Sugar And The Method Thereof}Rice beverage composition containing functional sugar and manufacturing method thereof {Rice Beverage Composition Containing Functional Sugar And The Method Thereof}

본 발명은 기능성 당을 함유하는 쌀 음료 조성물 및 그 제조방법에 관한 것이다.The present invention relates to a rice beverage composition containing the functional sugar and a method for producing the same.

일반적으로, 유산균은 오래 전부터 요쿠르트, 버터, 치즈와 같은 유가공 식품에서 중요한 역할을 담당하고 있으며, 유산균을 이용한 발효 식품은 특유의 풍미와 생성된 유산에 의한 우수한 보존성, 단백질의 부분 분해에 의한 소화 흡수성 향상 등 기호적, 영양적인 우수성을 나타내며 전세계를 통하여 엄청난 시장 규모를 형성하고 있다.In general, lactic acid bacteria have played an important role in dairy foods such as yogurt, butter and cheese for a long time, and fermented foods using lactic acid bacteria have peculiar flavor and excellent preservation by the resulting lactic acid and digestive absorption by partial decomposition of protein. It represents symbolic and nutritional excellence, such as improvement, and forms a huge market size throughout the world.

특히, 장내 정상 세균총의 유지, 장내 이상 발효의 개선, 장내 부패균에 의해 생성되는 독성물질의 무독화 작용, 칼슘의 흡수 촉진 등 유산균의 여러가지 기능적 작용과 변비, 고혈압, 피부염, 위장염 등의 증상에 효과가 밝혀짐으로써, 국내에서는 1971년 국내 업체에서 액상 발효유 생산을 시작으로 다양한 제품이 개발되어 왔다. In particular, it is effective for various functional effects of lactic acid bacteria including constipation, hypertension, dermatitis, gastroenteritis, such as maintenance of normal intestinal flora in the intestine, improvement of abnormal intestinal fermentation, detoxification of toxic substances produced by intestinal rot, and promotion of calcium absorption. As it turns out, a variety of products have been developed in Korea in 1971 starting with the production of liquid fermented milk in domestic companies.

특히, 근래에는 다양한 유산균주에 대한 연구가 시작되면서, 쌀, 콩, 야채 등의 식물성 원료를 발효한 유산균 음료가 개발되어 제품화 되고 있다. 그 중에서도 한국인에게 이용성, 기호성 및 기능성이 우수한 쌀에 유산균을 접종하여 발효하는 공정에 대한 연구가 활발히 이루어지고 있다.In particular, recently, as research on various lactic acid bacteria began, lactic acid bacteria beverages fermented from vegetable raw materials such as rice, beans and vegetables have been developed and commercialized. Among them, studies are being actively conducted on the process of inoculating fermented lactic acid bacteria into rice having excellent usability, palatability and functionality among Koreans.

팔라티노스(palatinose, isomaltulose, 6-O-α-D-glucopyranosyl-D-fructose)는 봉밀에 미량 포함된 천연의 당질 1-3)이고, 비우식원성(충치의 원인이 되지 않는)의 천연 감미료로서, 1950년대 독일의 Offstein(Pfalz주, 라틴 이름 Palatin)에서 팔라티노스 생산균이 발견된 이래, 세계 각국에서 연구·개발이 이루어져 왔다. 1984년, 생물 반응기 기술에 의하여 일본의 미쯔이제당 주식회사가 공업 생산을 시작하여, 현재는 일본을 비롯하여, 동아시아 및 유럽일부 국가에서 사용되고 있다. 팔라티노스는 설탕과 마찬가지로 포도당과 과당이 결합한 이당류로 설탕을 원료로 하여 제조하나 원료인 설탕은 포도당과 과당이 α-1, 2 결합으로 결합한 비환원당이지만, 팔라티노스는 α-1, 6 결합으로 결합한 환원 당으로 기존의 설탕과 액상과당에 비하여 천천히 흡수되어 급격한 혈당상승을 막아 주는 기능으로 주목받고 있다. Palatine, isomaltulose (6-O-α-D-glucopyranosyl-D-fructose) is a natural sweetener that is contained in traces of wheat in small amounts and is a natural sweetener of non-cartoid nature (which does not cause tooth decay). Since the discovery of palatinose producing bacteria in Offstein (Pfalz, Latin name Palatin) in the 1950s, research and development has been conducted in various countries around the world. In 1984, Mitsui Sugar Co., Ltd. of Japan started industrial production by bioreactor technology, and is currently used in Japan, East Asia and some parts of Europe. Like sugar, palatinose is a disaccharide that combines glucose and fructose. It is made from sugar as a raw material, but sugar is a non-reducing sugar in which glucose and fructose are combined with α-1 and 2 bonds. As a sugar, it is absorbed slowly compared to conventional sugars and liquid fructose and is attracting attention as a function to prevent a rapid blood sugar rise.

대한민국 등록특허 제0527419호에는 쌀당화액에 비피더스균과 유산균을 호화시킨 식품조성물이 있고, 대한민국 공개특허 제 1998-0075079호에 알파미분에 탈지분유를 기본으로 하여 유산발효시키고 과일엑기스를 주성분으로 한 쌀 요구르트가 개시된바 있었으나, 쌀과 우유가 완전히 호화되지 못하여 쌀과 우유가 이질적으로 촉감되어 식품을 음미할 때 이물질이 감지되는 문제점이 있었다. 또한 대한민국 등록특허 제 560988호에 쌀분말을 직접 첨가한 후 유산균을 접종하여 발효시키는 기능성 요구르트의 제조방법을 개시하고 있으나 쌀 성분의 함량이 곡분 냄새와 발효취가 어울리지 않아 기호성이 열악하다는 단점이 있으며, 대한민국 등록특허 제1069513호에 쌀성분의 함량을 높인 쌀 요구르트의 제조방법을 개시하고 있으나 유산균 발효액에 쌀 시럽을 혼합함으로써 실질적인 쌀 발효는 이루어지지 못한 문제점이 있었다. Republic of Korea Patent No. 0527419 has a food composition obtained by gelatinizing bifidus and lactic acid bacteria in rice saccharification liquid, and Republic of Korea Patent Publication No. 1998-0075079 based on skim milk powder in alpha fine powder based on the fermentation of lactic acid and fruit extract Although rice yogurt has been disclosed, there is a problem that the foreign matter is detected when the food is not savored because the rice and milk are not completely luxury, the rice and milk are heterogeneously tactile. In addition, Korean Patent No. 560988 discloses a method for preparing a functional yoghurt in which fermented by inoculating lactic acid bacteria after directly adding rice powder, but has a disadvantage in that palatability and poor fermentation odor are poor in rice content. , Republic of Korea Patent No. 1069513 discloses a method for producing a rice yogurt with a high content of rice, but there is a problem that the actual rice fermentation was not achieved by mixing the rice syrup in the lactic acid bacteria fermentation broth.

따라서 특화된 쌀 발효공정의 개발을 통해 기존의 쌀 요구르트 보다 2내지 3배의 쌀 함량을 높인 쌀 요구르트의 개발을 모색할 필요가 있다.Therefore, it is necessary to seek the development of rice yogurt with a rice content of 2 to 3 times higher than conventional rice yogurt through the development of specialized rice fermentation process.

KR0527419 BKR0527419 B KR1998-0075079 AKR1998-0075079 A KR560988 BKR560988 B KR1069513 BKR1069513 B

본 발명의 목적은, 쌀 발효공정을 통해 쌀 고유의 풍미와 맛을 증진시키고, 기능성 당을 함유하여 기호도와 관능성이 우수하며, 장시간 동안 유산균을 함유하는 쌀 음료 조성물 및 그 제조방법을 제공하는 것이다.It is an object of the present invention to enhance the flavor and taste of rice through a fermentation process of rice, and to provide a rice beverage composition containing lactic acid bacteria for a long time, having a functional sugar, excellent taste and functionality, and a method for producing the same will be.

본 발명은 동결건조된 락토바실러스 플란타룸(Lactobacillus plantarum), 비피도박테리움(Bifidobacterium), 락토바실러스 불가리커스(Lactobacillus bularicus) 혼합 분말 유산균; 쌀 농축액; 우유; 탈지분유; 팔라티노스; 펙틴; 구연산; 식이섬유 및 정제수를 포함하는 기능성 당을 함유하는 쌀 음료 조성물을 제공한다.The present invention is lyophilized Lactobacillus plantarum ( Lactobacillus plantarum), Bifidobacterium (Bifidobacterium), Lactobacillus Bulgaria carcass (Lactobacillus bularicus) mixed powder of lactic acid bacteria; Rice concentrate; milk; Skim milk powder; Palatinose; pectin; Citric acid; Provided is a rice beverage composition containing a functional sugar comprising dietary fiber and purified water.

또한, 본 발명은 쌀 음료 조성물 제조방법에 있어서,In addition, the present invention is a rice beverage composition manufacturing method,

(A) 쌀 농축액을 제조하는 단계;(A) preparing a rice concentrate;

(B) 우유 15 내지 40 중량%, 탈지분유 1 내지 4 중량%를 배합탱크에서 배합한 뒤 상기 (A)단계에서 제조된 쌀 농축액을 8 ~ 15 중량%를 배합탱크에 투입하여 배합하여 교반한 후, 살균 냉각하여 제 1 혼합물을 만드는 단계; 및(B) 15 to 40% by weight of milk and 1 to 4% by weight of skim milk powder in a mixing tank, followed by mixing by adding 8 to 15% by weight of the rice concentrate prepared in step (A) to the mixing tank and stirring After, sterilizing cooling to make a first mixture; And

(C) 팔라티노스 1 내지 5 중량%, 펙틴 0.01 내지 0.3 중량%, 구연산 0.0005 내지 0.1 중량%, 식이섬유 0.1 내지 5 중량% 및 잔량의 정제수를 배합탱크에 투입하여 배합하여 교반한 후, 살균 냉각하여 팔라티노스 시럽을 제조하는 단계;(C) 1 to 5% by weight of palatinose, 0.01 to 0.3% by weight of pectin, 0.0005 to 0.1% by weight of citric acid, 0.1 to 5% by weight of dietary fiber and the remaining amount of purified water were added to the mixing tank, followed by mixing and stirring, followed by sterilization cooling. Preparing palatinose syrup;

(D) (B)단계의 제 1 혼합물에 동결건조된 락토바실러스 플란타룸(Lactobacillus plantarum), 비피도박테리움(Bifidobacterium), 락토바실러스 불가리커스(Lactobacillus bularicus) 혼합 분말 유산균을 접종하여 36 내지 45℃ 온도에서 3 내지 12 시간 동안 교반하여 발효시켜, 유산균 발효액을 제조하는 단계;(D) (B) The freeze-dried in the first step a mixture of Lactobacillus Planta room (Lactobacillus plantarum), Bifidobacterium (Bifidobacterium), Lactobacillus Bulgaria and Caicos (Lactobacillus bularicus ) inoculated mixed powder lactic acid bacteria and fermented by stirring for 3 to 12 hours at a temperature of 36 to 45 ℃, to prepare a lactic acid bacteria fermentation broth;

(E) 상기 (D) 단계에서 유산균 발효액의 적정산도(titratable acidity)가 0.75%에 도달하면 상기 (C)에서 제조된 팔라티노스 시럽과 혼합하여 100 내지 300 kg/cm2의 압력으로 교반시키면서 균질화하는 단계;를 포함하는 쌀 음료 제조방법을 제공한다.(E) when the titer (titratable acidity) of the lactic acid bacteria fermentation broth in step (D) reaches 0.75%, it is mixed with palatinose syrup prepared in (C) and homogenized while stirring at a pressure of 100 to 300 kg / cm 2 It provides a rice beverage manufacturing method comprising a.

또한 본 발명은 상기 제조방법으로 제조된 기능성 당 및 유산균을 함유하는 쌀 음료 또는 쌀 요구르트를 제공한다. In another aspect, the present invention provides a rice beverage or rice yogurt containing the functional sugar and lactic acid bacteria prepared by the above production method.

본 발명의 쌀 음료 조성물은 쌀의 효소처리 및 당화과정을 통해 농축액을 제조하여 기존의 곡분 냄새와 요구르트 발효취가 서로 어울리지 않아 기호성이 떨어지는 단점을 해결하고, 쌀이 첨가된 요구르트가 아닌 쌀 자체를 발효시켜 만듦으로써 쌀 함유량을 높여 맛, 색상 및 풍미 등의 관능성이 우수한 유산균 발효제품으로 사용될 수 있다.The rice beverage composition of the present invention is to prepare a concentrate through the enzymatic treatment and saccharification process of the rice to solve the disadvantages of dropping palatability because the existing grain odor and yogurt fermentation odor does not match each other, the rice itself is not yogurt added rice By making it fermented, it can be used as a lactic acid bacterium fermentation product having excellent functionality such as taste, color and flavor by increasing rice content.

또한, 본 발명의 쌀 음료 조성물은 액상과당 및 설탕을 이용하지 않고, 이를 대체하여 팔라티노스를 사용함으로써, 당도는 보완하면서 급격한 혈당상승을 억제하는 효과를 가진다.In addition, the rice beverage composition of the present invention does not use the liquid fructose and sugar, by using this instead of palatinose, has the effect of suppressing the rapid blood sugar rise while supplementing the sugar.

또한, 본 발명은 쌀의 맛과 영양을 모두 포함하며, 현대인에게 부족하기 쉬운 유산균(유산균 수 106cell/ml이상)을 동시에 섭취할 수 있어 상품성 및 수용성이 우수한 조성물을 제공함으로써, 국내산 쌀 소비 확대를 활성화 할 수 있다. In addition, the present invention includes both the taste and nutrition of rice, at the same time can be easily ingested lactic acid bacteria (lactic acid bacteria more than 10 6 cell / ml) that is easy for modern people to provide a composition having excellent commerciality and water solubility, domestic rice consumption You can activate magnification.

이하, 본 발명을 더욱 상세히 설명한다.Hereinafter, the present invention will be described in more detail.

본 발명은 동결건조된 락토바실러스 플란타룸(Lactobacillus plantarum), 비피도박테리움(Bifidobacterium), 락토바실러스 불가리커스(Lactobacillus bularicus) 혼합 분말 유산균; 쌀 농축액; 우유; 탈지분유; 팔라티노스; 펙틴; 구연산; 식이섬유 및 정제수를 포함하는 쌀 음료 조성물에 관한 것으로, 보다 상세하게는 상기 쌀 음료 조성물은 조성물 총 중량에 대하여, 동결건조된 락토바실러스 플란타룸(Lactobacillus plantarum), 비피도박테리움(Bifidobacterium), 락토바실러스 불가리커스(Lactobacillus bularicus) 혼합 분말 유산균 0.005 내지 0.1 중량%; 쌀 농축액 8 내지 15중량%; 우유 15 내지 40 중량%; 탈지분유 1 내지 4 중량%; 팔라티노스 1 내지 5 중량%; 펙틴 0.01 내지 0.3 중량%; 구연산 0.0005 내지 0.1 중량%; 식이섬유 0.1 내지 5 중량%; 및 잔량의 정제수를 포함하는 쌀 음료 조성물을 제공한다.
The present invention is lyophilized Lactobacillus plantarum ( Lactobacillus plantarum), Bifidobacterium (Bifidobacterium), Lactobacillus Bulgaria carcass (Lactobacillus bularicus) mixed powder of lactic acid bacteria; Rice concentrate; milk; Skim milk powder; Palatinose; pectin; Citric acid; A rice beverage composition comprising dietary fiber and purified water, and more specifically, the rice beverage composition is based on the total weight of the composition, lyophilized Lactobacillus plantarum ( Lactobacillus plantarum), Bifidobacterium (Bifidobacterium), Lactobacillus Bulgaria and Caicos (Lactobacillus bularicus ) mixed powder lactic acid bacteria 0.005 to 0.1% by weight; Rice concentrate 8 to 15% by weight; 15-40% milk; 1 to 4 wt% skim milk powder; 1-5% by weight of palatinose; 0.01-0.3 wt% pectin; Citric acid 0.0005 to 0.1 wt%; 0.1 to 5% by weight of dietary fiber; And it provides a rice beverage composition comprising a residual amount of purified water.

상기 동결건조된 락토바실러스 플란타룸(Lactobacillus plantarum), 비피도박테리움(Bifidobacterium), 락토바실러스 불가리커스(Lactobacillus bularicus) 혼합 분말 유산균은 락토바실러스 불가리커스(Lactobacillus bularicus), 비피도박테리움(Bifidobacterium), 락토바실러스 비피도박테리움(Bifidobacterium)가 3 ~ 7 : 2 ~ 4 : 0.5 ~ 2 중량비율로 혼합되는 것이 바람직하며, 보다 바람직하게는 5 ~ 7 : 2 ~ 3 : 0.5 ~ 2의 중량비율로 혼합될 수 있으며, 6 : 2.5 : 1의 혼합비율로 혼합되는 것이 가장 바람직하다. 상기 동결건조된 락토바실러스 플란타룸(Lactobacillus plantarum), 비피도박테리움(Bifidobacterium), 락토바실러스 불가리커스(Lactobacillus bularicus) 혼합 분말 유산균은 상기 범위의 비율로 포함될 때, 유산균의 생장촉진에 효과적이고, 쌀 음료의 관능성이 우수하다.
Lactobacillus lyophilized Lactobacillus plantarum plantarum), Bifidobacterium (Bifidobacterium), Lactobacillus Bulgaria and Caicos (Lactobacillus bularicus mixed powder lactic acid bacteria Lactobacillus bularicus ), Bifidobacterium ( Bifidobacterium ), Lactobacillus Bifidobacterium ( Bifidobacterium ) is preferably mixed in a weight ratio of 3-7: 2-4: 0.5-2, more preferably 5-7: 2 It may be mixed at a weight ratio of ˜3: 0.5 to 2, and most preferably mixed at a mixing ratio of 6: 2.5: 1. Lactobacillus lyophilized Lactobacillus plantarum plantarum), Bifidobacterium (Bifidobacterium), Lactobacillus Bulgaria and Caicos (Lactobacillus bularicus ) mixed powder lactic acid bacteria, when included in the ratio of the above range, is effective in promoting the growth of lactic acid bacteria, and the functionality of rice beverage is excellent.

상기 동결건조된 락토바실러스 플란타룸(Lactobacillus plantarum), 비피도박테리움(Bifidobacterium), 락토바실러스 불가리커스(Lactobacillus bularicus) 혼합 분말 유산균은 조성물 총 중량에 대하여 0.005 내지 0.1 중량%로 포함되는 것이 바람직하고, 상기 범위로 포함될 때 효율적인 발효 및 쌀 음료 조성물 제조 후 장시간 106 cfu/ml 이상의 유산균 생존량을 유지할 수 있다.Lactobacillus lyophilized Lactobacillus plantarum plantarum), Bifidobacterium (Bifidobacterium), Lactobacillus Bulgaria and Caicos (Lactobacillus bularicus ) mixed powder lactic acid bacteria is preferably included in 0.005 to 0.1% by weight based on the total weight of the composition, when included in the above range can be maintained for lactic acid bacteria survival of 10 6 cfu / ml or more for a long time after the production of efficient fermentation and rice beverage composition .

상기 비피도박테리움은 강한 내산성을 지니고 있으면서, 소장 내에서 점착성도 다른 프로바이오틱에 비해 월등하여 면역 능력 강화와 변비개선에 등에 효과가 있는 균주이다. 상기 비피도박테리움은 비피도박테리움 아도레스센티스 (B.adolescentis), 비피도박테리움 비피덤(B. bifidum), 비피도박테리움 브레브 (B.breve), 비피도박테리움 인판티스(B. infantis), 비피도박테리움 롱검(B.longum)으로부터 선택된 1종 또는 2종 이상인 것이 바람직하나, 이에 한정되지는 않는다.
The Bifidobacterium is a strain having strong acid resistance and excellent adhesion in the small intestine compared to other probiotics and is effective in strengthening immunity and improving constipation. The Bifidobacterium is Bifidobacterium adorescentis ( B.adolescentis ), Bifidobacterium bifidem ( B. bifidum ), Bifidobacterium breb ( B.breve ), Bifidobacterium infantis ( B. infantis ), Bifidobacterium long gum ( B. longum ) is preferably selected from one or two or more, but is not limited thereto.

상기 쌀 농축액은 상기 쌀 농축액은 50 Brix기준으로 하여 8 내지 15 중량%로 포함되는 것이 바람직하며(쌀 함량으로 환원시 40 내지 75 중량%를 의미한다.)
The rice concentrate is preferably contained in an amount of 8 to 15% by weight based on 50 Brix (refer to 40 to 75% by weight when reduced to rice content).

상기 쌀 농축액은 다음과 같은 공정에 의해 제조될 수 있다. The rice concentrate may be prepared by the following process.

(1) 현미와 백미를 정선한 후 150 ∼ 230 ℃에서 10 ∼ 20 분간 볶는, 볶음 공정 단계;(1) a roasting step of selecting brown and white rice and then roasting at 150 to 230 ° C. for 10 to 20 minutes;

(2) 상기 (1)단계에서 얻은 현미와 백미를 10 ∼ 50 메쉬(mesh)로 분쇄하여 1:9 내지 2:8 중량비율로 혼합하여, 현미 및 백미 혼합원료에 대하여 4 ∼ 8 배의 정제수를 가한 후 100℃로 가열하면서 교반시키는, 교반 단계; (2) grinding the brown rice and the white rice obtained in step (1) into 10 to 50 mesh and mixing them at a weight ratio of 1: 9 to 2: 8, and 4 to 8 times purified water with respect to the brown and white rice mixed raw materials. After the addition of the stirring step of stirring while heating to 100 ℃;

(3) 상기 (2)단계에서 교반시켜 얻은 재료를 88 ∼ 96℃로 냉각시키고 액화효소인 박테리아 α-아밀라아제(Bacteria α-Amylase)를 상기 재료 100 중량부 대비 0.02 ∼ 0.06 중량부를 첨가하여 10 ∼ 30분간 효소 분해시켜 당도 6 ∼ 11 브릭스(Brix)의 1차 효소 분해액을 얻는, 1차 효소 분해 단계;(3) Cool the material obtained by stirring in step (2) to 88-96 ° C and add 0.02-0.06 parts by weight of liquefied enzyme bacteria α-Amylase to 100 parts by weight of the material to 10- A first enzymatic digestion step of enzymatic digestion for 30 minutes to obtain a primary enzymatic digestion solution with a sugar content of 6 to 11 Brix;

(4) 상기 (3)단계에서 얻은 1차 효소 분해액을 다시 50 ∼ 60℃로 냉각한 후, 상기 1차 효소 분해액 100 중량부 대비 당화효소인 글루코아밀라아제(glucoamylase)를 0.05 ∼ 0.25 중량부, 단백질 분해효소인 프로테아제(protease)를 0.1 ∼ 0.3 중량부, 펙틴 분해효소를 0.05 ∼ 0.25 중량부로 첨가하여 3 ∼ 8 시간 효소 분해시켜, 당도 8 ∼ 13 브릭스(Brix)의 2차 효소 분해액을 얻는, 2차 효소 분해 단계;(4) After cooling the primary enzyme digestion solution obtained in step (3) again to 50-60 ° C., 0.05 to 0.25 parts by weight of glucoamylase, a glycosylating enzyme, relative to 100 parts by weight of the primary enzyme digestion solution. , 0.1 to 0.3 parts by weight of protease, a protease, and 0.05 to 0.25 parts by weight of pectinase, were added for enzyme digestion for 3 to 8 hours, and the second enzyme digestion solution having a sugar content of 8 to 13 Brix was obtained. Secondary enzymatic digestion step;

(5) 상기 (4 )단계에서 얻어진 2차 효소 분해액을 100℃로 5 ∼ 10 분간 가열시켜 효소를 실활시키고, 아울러 효소반응을 중지시키는, 효소 실활 단계;및(5) an enzyme inactivation step of heating the secondary enzyme digestion solution obtained in step (4) at 100 ° C. for 5 to 10 minutes to inactivate the enzyme and stop the enzymatic reaction; and

(6) 상기 (5)단계에서 효소 실활 단계를 거친 2차 효소 분해액을 40 ∼ 120 메쉬(mesh)로 1차 여과 후, 2차 원심 분리하여 잔류물을 제거하여, 당도 11 ∼ 13 브릭스(Brix)의 쌀 농축액을 제조하는 단계를 포함하여, 쌀 농축액을 제조하는 것이 바람직하다.
(6) After the first filtration of the second enzymatic digestion solution, which had undergone the enzyme inactivation step in step (5), with 40 to 120 mesh, the residue was removed by secondary centrifugation to obtain a sugar content of 11 to 13 brix ( It is preferred to prepare a rice concentrate, including preparing a rice concentrate of Brix).

상기 (1)단계에서, 볶음 온도가 150℃ 이하이면, 볶음 시간이 너무 많이 소요되고, 색깔과 풍미가 잘 나지 않는 반면, 230℃ 이상이면 쉽게 타 버리므로 볶음 온도에 유의하여 진행해야 한다.In the step (1), if the roasting temperature is 150 ° C or less, the roasting takes too much time, and the color and flavor are not good, whereas if the roasting temperature is 230 ° C or higher, the roasting temperature is easily burned.

상기 (2)단계에서, 상기 현미와 백미의 혼합비율은 제품의 용도에 따라 달라질 수 있으며, 상기 현미와 백미는 1:9 내지 5:5의 중량비율로 혼합하여 제조하는 것이 바람직하며, 보다 바람직하게는 1:9 내지 2:8 중량비율로 혼합하여 사용할 수 있다. 영양가 측면을 고려할 경우 현미의 비율이 높은 쪽으로 혼합할 수 있고, 가격과 영양가 모두를 고려할 때, 상기 범위가 가장 적절하다.
In the step (2), the mixing ratio of the brown rice and the white rice may vary depending on the use of the product, the brown rice and the white rice is preferably prepared by mixing in a weight ratio of 1: 9 to 5: 5, more preferably Preferably it may be used by mixing in a weight ratio of 1: 9 to 2: 8. Considering the nutritional value, the ratio of brown rice can be mixed to the higher side, and the above range is most appropriate when considering both the price and the nutritional value.

상기 (6)단계에서는 상기 (5)단계에서 효소 실활 단계를 거친 2차 효소 분해액을 40 ∼ 120 메쉬(mesh)로 1차 여과 후, 2차 원심 분리하여 잔류물을 제거하고 최종수율 80% 이상의 당도 11 ∼ 13 브릭스(Brix)의 맑고 깨끗한 액인, 쌀 농축액을 얻을 수 있다.
In the step (6), the second enzymatic digestion solution, which has undergone the enzyme inactivation step in the step (5), is first filtered through 40 to 120 mesh, and then the residue is removed by secondary centrifugation to obtain a final yield of 80%. The rice concentrate which is a clear and clean liquid of the above-mentioned sugars 11-13 Brix can be obtained.

상기 쌀 농축액 제조 방법에서, (6)단계 이후, (7) 제조된 쌀 농축액을 농축하는 단계를 추가로 포함할 수 있으며, 농축 방법은 당 업계에서 통상적으로 이용되는 방법이라면 어느 것이 사용 가능하다.In the rice concentrate production method, after step (6), (7) may further comprise the step of concentrating the prepared rice concentrate, any concentration method can be used as long as it is commonly used in the art.

상기 (7)단계에서 농축된 쌀 농축액은 40 ∼ 60 브릭스(Brix)인 것이 바람직하며, 보다 바람직하게는 50 브릭스(Brix)일 수 있다.
Rice concentrate concentrated in the step (7) is preferably 40 to 60 Brix (Brix), more preferably 50 Brix (Brix) may be.

또한, 상기 쌀 음료 조성물은 설탕이나 액상과당를 포함하지 않는 것을 특징으로 한다. 종래의 요구르트 조성물은 일반적으로 설탕이나 액상과당의 사용을 통해 감미도와 제품 물성을 부여하고 있으나, 이는 높은 칼로리 및 급격한 혈당상승의 원인이 되고 있다. 본 발명의 쌀 음료 조성물은 설탕이나 액상과당을 대신하여 기능성 당인 팔라티노스를 함유함으로써 요구르트 고유의 풍미와 맛을 유지시키며, 감미도를 보완하는 효과를 제공한다.
In addition, the rice beverage composition is characterized in that it does not contain sugar or liquid fructose. Conventional yogurt compositions generally provide sweetness and product properties through the use of sugar or liquid fructose, but this causes high calories and rapid blood sugar rise. The rice beverage composition of the present invention maintains the flavor and taste of yogurt by supplementing sweetness by containing palatinose which is a functional sugar instead of sugar or liquid fructose.

팔라티노스는 이소말툴로스(isomaltulose)라고도 불리우며, 글루코스가 프락토오스에 α-1,6-글루코실 결합함으로써 구성된 2당이다. 팔라티노스는 소장 하부에 많이 존재하는 포만 호르몬인 GLP-1을 분비시키는 세포를 자극시켜, 팔라티노스를 섭취하였을 때 설탕이나 전분 등 다른 당을 섭취했을 때보다 많이 GLP-1을 분비시키게 된다. 일반적인 당질은 소화 흡수가 빠르기 때문에 상당한 양을 섭취하지 않으면 소장 하부에는 도달하지 않으나, 팔라티노스는 소화 흡수가 늦기 때문에, GLP-1을 분비하는 세포를 자극하는 것이 가능하다. 따라서 급격한 혈당치(포도당) 상승을 억제하고, 지속적인 포만감을 유지하여, 혈당이 낮은 상태에서 일어나는 공복감을 억제할 수 있다. 상기 팔라티노스는 상기 쌀 음료 조성물 총 중량에 대하여 1 내지 5 중량%로 포함되는 것이 바람직하며, 상기 범위 내로 포함 되었을 때 쌀 음료의 감미가 우수하며, 유산균의 생장촉진에 효과적이다.
Palatinos, also called isomaltulose, is a disaccharide formed by glucose binding α-1,6-glucosyl to fructose. Palatinos stimulates cells that secrete GLP-1, a satiety hormone found in the lower part of the small intestine, and secrete more GLP-1 when consuming palatinose than when taking other sugars such as sugar or starch. Normal carbohydrates do not reach the lower part of the small intestine unless a significant amount is ingested because of fast digestion absorption, but since palatinose is slow in digestion absorption, it is possible to stimulate cells secreting GLP-1. Therefore, it is possible to suppress a sudden rise in blood sugar level (glucose), maintain a feeling of full satiety, and suppress a fasting feeling occurring in a state where blood sugar is low. The palatinose is preferably contained in 1 to 5% by weight based on the total weight of the rice beverage composition, when included in the range is excellent in the sweetness of the rice beverage, it is effective in promoting the growth of lactic acid bacteria.

또한, 상기 식이섬유는 당업계에서 사용되는 것이면 제한 없이 사용가능하나, 치커리 식이섬유, 썬화이버(구아검을 효소분해하여 만든 수용성 식이섬유) 또는 이들의 혼합물을 사용하는 것이 바람직하다. 상기 식이섬유는 조성물 총 중량에 대하여 0.1 내지 5 중량%로 포함되는 것이 바람직하며, 상기 범위 내로 포함되면, 유산균과 함께 장내에서 정장효과를 높일 수 있다.
In addition, the dietary fiber can be used without limitation as long as it is used in the art, it is preferable to use chicory dietary fiber, sun fiber (water soluble fiber made by enzymatic decomposition of guar gum) or a mixture thereof. The dietary fiber is preferably included in 0.1 to 5% by weight relative to the total weight of the composition, if included in the above range, can increase the intestinal effect with the lactic acid bacteria.

본 발명의 쌀 음료 조성물에 포함되는 정제수는 조성물 총 중량이 100 중량%가 되도록 잔량 포함된다.
Purified water contained in the rice beverage composition of the present invention is contained in the remaining amount so that the total weight of the composition is 100% by weight.

또한, 본 발명의 쌀 음료 조성물에는 본 발명의 목적 및 효과를 벗어나지 않는 범위 내에서 당 업계에서 통상적으로 사용되는 첨가제를 추가로 포함할 수 있다. 예를 들면, 상기 첨가제는 유화제, 색소, pH 조절제, 완충제, 비타민, 감미료, 향미료 등을 들 수 있다.
In addition, the rice beverage composition of the present invention may further include additives commonly used in the art within the scope without departing from the object and effect of the present invention. For example, the additives include emulsifiers, colorants, pH adjusters, buffers, vitamins, sweeteners, flavors and the like.

상기 쌀 음료는 쌀 요구르트인 것이 보다 바람직하나, 이에 한정되는 것은 아니다.
The rice drink is more preferably rice yogurt, but is not limited thereto.

본 발명은 쌀 음료 제조방법에 있어서,The present invention in the rice beverage manufacturing method,

(A) 쌀 농축액을 제조하는 단계;(A) preparing a rice concentrate;

(B) 우유 15 내지 40 중량%, 탈지분유 1 내지 4 중량%를 배합탱크에서 배합한 뒤 상기 (A)단계에서 제조된 쌀 농축액을 8 ~ 15 중량%를 배합탱크에 투입하여 배합하여 교반한 후, 살균 냉각하여 제 1 혼합물을 만드는 단계; 및(B) 15 to 40% by weight of milk and 1 to 4% by weight of skim milk powder in a mixing tank, followed by mixing by adding 8 to 15% by weight of the rice concentrate prepared in step (A) to the mixing tank and stirring After, sterilizing cooling to make a first mixture; And

(C) 팔라티노스 1 내지 5 중량%, 펙틴 0.01 내지 0.3 중량%, 구연산 0.0005 내지 0.1 중량%, 식이섬유 0.1 내지 5 중량% 및 잔량의 정제수를 배합탱크에 투입하여 배합하여 교반한 후, 살균 냉각하여 팔라티노스 시럽을 제조하는 단계;(C) 1 to 5% by weight of palatinose, 0.01 to 0.3% by weight of pectin, 0.0005 to 0.1% by weight of citric acid, 0.1 to 5% by weight of dietary fiber and the remaining amount of purified water were added to the mixing tank, followed by mixing and stirring, followed by sterilization cooling. Preparing palatinose syrup;

(D) (B)단계의 제 1 혼합물에 동결건조된 락토바실러스 플란타룸(Lactobacillus plantarum), 비피도박테리움(Bifidobacterium), 락토바실러스 불가리커스(Lactobacillus bularicus) 혼합 분말 유산균을 접종하여 36 내지 45℃ 온도에서 3 내지 12 시간 동안 교반하여 발효시켜, 유산균 발효액을 제조하는 단계;(D) (B) The freeze-dried in the first step a mixture of Lactobacillus Planta room (Lactobacillus plantarum), Bifidobacterium (Bifidobacterium), Lactobacillus Bulgaria and Caicos (Lactobacillus bularicus ) inoculated mixed powder lactic acid bacteria and fermented by stirring for 3 to 12 hours at a temperature of 36 to 45 ℃, to prepare a lactic acid bacteria fermentation broth;

(E) 상기 (D) 단계에서 유산균 발효액의 적정산도(titratable acidity)가 0.75%에 도달하면 상기 (C)에서 제조된 팔라티노스 시럽과 혼합하여 100 내지 300 kg/cm2의 압력으로 교반시키면서 균질화하는 단계;를 포함하는 쌀 음료 제조방법을 제공한다.
(E) when the titer (titratable acidity) of the lactic acid bacteria fermentation broth in step (D) reaches 0.75%, it is mixed with palatinose syrup prepared in (C) and homogenized while stirring at a pressure of 100 to 300 kg / cm 2 It provides a rice beverage manufacturing method comprising a.

상기 (A) 쌀 농축액을 제조하는 단계는 상기 기재한 내용을 원용한다.
The step of preparing the (A) rice concentrate uses the contents described above.

상기 동결건조된 락토바실러스 플란타룸(Lactobacillus plantarum), 비피도박테리움(Bifidobacterium), 락토바실러스 불가리커스(Lactobacillus bularicus) 혼합 분말 유산균은 락토바실러스 불가리커스(Lactobacillus bularicus), 비피도박테리움(Bifidobacterium), 락토바실러스 비피도박테리움(Bifidobacterium)가 3 ~ 7 : 2 ~ 4 : 0.5 ~ 2 중량비율로 혼합되는 것이 바람직하며, 보다 바람직하게는 5 ~ 7 : 2 ~ 3 : 0.5 ~ 2의 중량비율로 혼합될 수 있으며, 6 : 2.5 : 1의 중량비율로 혼합되는 것이 가장 바람직하다. 상기 동결건조된 락토바실러스 플란타룸(Lactobacillus plantarum), 비피도박테리움(Bifidobacterium), 락토바실러스 불가리커스(Lactobacillus bularicus) 혼합 분말 유산균은 상기 범위의 비율로 포함될 때, 유산균의 생장촉진에 효과적이고, 쌀 음료의 관능성이 우수하다.
Lactobacillus lyophilized Lactobacillus plantarum plantarum), Bifidobacterium (Bifidobacterium), Lactobacillus Bulgaria and Caicos (Lactobacillus bularicus mixed powder lactic acid bacteria Lactobacillus bularicus ), Bifidobacterium ( Bifidobacterium ), Lactobacillus Bifidobacterium ( Bifidobacterium ) is preferably mixed in a weight ratio of 3-7: 2-4: 0.5-2, more preferably 5-7: 2 It may be mixed at a weight ratio of ˜3: 0.5 to 2, and most preferably mixed at a weight ratio of 6: 2.5: 1. Lactobacillus lyophilized Lactobacillus plantarum plantarum), Bifidobacterium (Bifidobacterium), Lactobacillus Bulgaria and Caicos (Lactobacillus bularicus ) mixed powder lactic acid bacteria, when included in the ratio of the above range, is effective in promoting the growth of lactic acid bacteria, and the functionality of rice beverage is excellent.

상기 동결건조된 락토바실러스 플란타룸(Lactobacillus plantarum) 비피도박테리움(Bifidobacterium), 락토바실러스 불가리커스(Lactobacillus bularicus) 혼합 분말 유산균은 조성물 총 중량에 대하여 0.005 내지 0.1 중량%로 포함되는 것이 바람직하고, 상기 범위로 포함될 때 효율적인 발효 및 쌀 음료 조성물 제조 후 장시간 106 cfu/ml 이상의 유산균 생존량을 유지할 수 있다.
Lactobacillus lyophilized Lactobacillus plantarum plantarum) Bifidobacterium (Bifidobacterium), Lactobacillus Bulgaria and Caicos (Lactobacillus bularicus ) mixed powder lactic acid bacteria is preferably included in 0.005 to 0.1% by weight based on the total weight of the composition, when included in the above range can be maintained for lactic acid bacteria survival of 10 6 cfu / ml or more for a long time after the production of efficient fermentation and rice beverage composition .

또한, (B)단계의 살균 냉각하는 단계는 95℃에서 5분 동안 살균하고 10 내지 20℃에서 냉각시켜 이송하는 것이 바람직하며, 이에 한정되는 것은 아니다.In addition, sterilization cooling step (B) is preferably sterilized for 5 minutes at 95 ℃ and cooled to 10 to 20 ℃ transport, it is not limited thereto.

상기 (C)단계는 65 내지 70℃ 온수에서 팔라티노스, 펙틴, 구연산, 식이섬유를 용해한 후, 95℃에서 5분 동안 살균하고 10 내지 20℃에서 냉각시켜 이송하는 것이 바람직하다.In the step (C), after dissolving palatinose, pectin, citric acid, and dietary fiber in hot water at 65 to 70 ° C., sterilization at 95 ° C. for 5 minutes and cooling at 10 to 20 ° C. is preferably carried.

상기 (D) 단계에서, 상기 제 1 혼합물에 동결건조된 락토바실러스 플란타룸(Lactobacillus plantarum), 비피도박테리움(Bifidobacterium), 락토바실러스 불가리커스(Lactobacillus bularicus) 혼합 분말 유산균을 접종하고 36 내지 45℃ 온도에서 3 내지 12 시간 동안 교반하여 발효한다. 발효온도와 시간의 종료는 pH 4.3 내지 4.5 가 되는 때를 기준으로 하며 이는 각각 유산균 106 ~1010 cell/ml 정도로 접종하는 것으로 최적 발효조건이다. In the step (D), Lactobacillus plantarum lyophilized in the first mixture ( Lactobacillus plantarum), Bifidobacterium (Bifidobacterium), Lactobacillus Bulgaria and Caicos (Lactobacillus bularicus ) mixed powdered lactic acid bacteria and fermented by stirring for 3 to 12 hours at a temperature of 36 to 45 ℃. The end of fermentation temperature and time is based on when the pH is 4.3 to 4.5, which is the optimum fermentation conditions inoculated with lactic acid bacteria 10 6 ~ 10 10 cell / ml, respectively.

상기 (E)단계는 상기 유산균 발효액이 적정산도에 0.75%에 도달하면 (C)단계의 팔라티노스 시럽액과 혼합하여 100 내지 300 kg/cm2의 압력으로 균질화하는 단계이다.The step (E) is a step of homogenizing at 100 to 300 kg / cm 2 by mixing with the palatinose syrup solution of step (C) when the lactic acid bacteria fermentation broth reaches 0.75% in the appropriate acidity.

상기 (E)단계 이후, (F) 상기 균질화된 쌀 음료를 10 내지 20℃의 온도로 냉각시켜 이송하여 용기에 담아 포장하는 단계를 더 포함할 수 있으며, 이때 용기는 플라스틱 용기, 종이재 용기 등을 사용할 수 있다.
After the step (E), (F) the homogenized rice beverage may be further cooled and transported to a temperature of 10 to 20 ℃ packed in a container, wherein the container is a plastic container, a paper material container, etc. Can be used.

본 발명은 본 발명의 쌀 음료 제조방법에 의해 제조된 기능성 당 및 유산균을 함유하는 쌀 음료 또는 쌀 요구르트를 제공한다.
The present invention provides a rice beverage or rice yogurt containing the functional sugar and lactic acid bacteria produced by the rice beverage production method of the present invention.

이하에서, 본 발명을 실시예를 이용하여 더욱 상세하게 설명한다. 그러나, 하기의 실시예는 본 발명을 더욱 구체적으로 설명하기 위한 것으로서, 본 발명의 범위가 하기의 실시예에 의하여 한정되는 것은 아니다. 하기의 실시예는 본 발명의 범위 내에서 당업자에 의해 적절히 수정 또는 변경될 수 있다.Hereinafter, the present invention will be described in more detail using examples. However, the following examples are intended to further illustrate the present invention, and the scope of the present invention is not limited by the following examples. The following examples may be appropriately modified or changed by those skilled in the art within the scope of the present invention.

제조예Manufacturing example 1 : 쌀 농축액 제조 1: Manufacture of Rice Concentrate

현미와 백미를 정선한 후 200 ℃에서 15 분간 볶아서 색깔과 향을 부여하였다. 상기 볶은 현미와 백미를 10 ∼ 50 메쉬(mesh)로 분쇄하여 2:8 중량비율로 섞어 총 제조량의 10 중량% 평량 후, 상기 볶은 현미와 백미혼합원료에 원료량의 5 배의 정제수를 가한 후 100℃로 가열하면서 교반시켰다. 상기 교반시켜 얻은 현미와 백미 재료를 90℃로 냉각시켜 액화효소인 박테리아 α-아밀라아제(Bacteria α-Amylase)를 0.04 중량%를 첨가하여 20분간 효소 분해시켜 당도 6~11의 브릭스(Brix)의 1차 효소 분해액을 얻었다. 상기 1차 효소 분해액을 다시 50 ∼ 60℃로 냉각한 후, 당화효소인 글루코아밀라아제(glucoamylase)를 0.1 중량%, 단백질 분해효소인 프로테아제(protease)를 0.2 중량%, 펙틴 분해효소를 0.1 중량%로 첨가하여 6 시간 효소 분해시켜, 당도 8 ∼ 13 브릭스(Brix)의 2차 효소 분해된 액을 얻었다. 상기 2차 효소 분해된 액을 100℃로 10 분간 가열시켜 효소를 실활시키고, 아울러 효소반응을 중지시켰다. 상기 공정을 통하여 생성/얻어지고, 실활 공정을 거친 2차 효소 분해된 액을 40 ∼ 120 메쉬(mesh)로 1차 여과 후, 2차원심 분리하여 잔류물을 제거하고 최종수율 80% 또는 그 이상의 당도 11 ∼ 13 브릭스(Brix)의 쌀 농축액을 얻었다.After choosing brown and white rice, roasted for 15 minutes at 200 ℃ to give color and flavor. After grinding the roasted brown rice and white rice into 10 to 50 mesh (mixing) in a 2: 8 weight ratio, and after 10 wt% basis weight of the total amount of production, after adding 5 times the purified water of the raw material amount to the roasted brown rice and white rice mixed raw materials It stirred while heating to 100 degreeC. The agitated brown rice and white rice material was cooled to 90 ° C., and 0.04% by weight of liquefied enzyme Bacteria α-Amylase was added thereto to enzymatically digested for 20 minutes to obtain a Brix having a sugar content of 6-11. Primary enzyme digestion was obtained. After cooling the primary enzyme digestion liquid again to 50-60 ° C., 0.1% by weight of glycosylated glucoamylase, 0.2% by weight of protease (protease) and 0.1% by weight of pectinase After enzymatic digestion for 6 hours, a secondary enzymatically digested solution of Brix was found. The secondary enzymatically decomposed solution was heated to 100 ° C. for 10 minutes to inactivate the enzyme, and to stop the enzyme reaction. The primary enzymatically decomposed liquid produced / obtained through the above process and then deactivated was subjected to primary filtration to 40 to 120 mesh, followed by two-dimensional separation to remove the residue, and a final yield of 80% or more. A rice concentrate with a sugar content of 11 to 13 Brix was obtained.

상기 쌀 농축액을 농축하여 50 브릭스(brix)의 쌀 농축액을 얻었다.The rice concentrate was concentrated to obtain a rice brix of 50 brix.

실시예Example 1 및  1 and 비교예Comparative example 1 : 쌀  1: rice 음료 음료Drink drink 제조 Produce

실시예Example 1 One

우유 30 중량%, 탈지분유 2.0 중량%을 배합한 뒤, 상기 제조예 1로부터 얻은 50 브릭스(brix)의 쌀 농축액 8.0 중량%(쌀함량으로 40%)을 배합탱크에 투입하여 배합하여 교반한 후, 살균·냉각하여 제 1 혼합액을 제조하였다. After mixing 30% by weight of milk and 2.0% by weight of skimmed milk powder, 8.0 wt% (50% as a rice content) of 50 brix rice concentrate obtained from Preparation Example 1 was added to the mixing tank, followed by mixing and stirring. Sterilization and cooling were carried out to prepare a first mixed solution.

팔라티노스 4.0 중량%, 치커리식이섬유 2.0 중량%, 펙틴 0.05 중량%, 구연산 0.007 중량% 및 정제수 53.938 중량%를 배합탱크에 투입하여 배합하여 교반한 후, 살균 냉각하여 팔라티노스 시럽을 제조하였다. Palatinose 4.0% by weight, Chicory dietary fiber 2.0% by weight, pectin 0.05% by weight, citric acid 0.007% by weight and purified water 53.938% by weight were added to the mixing tank, the mixture was stirred and then sterilized and cooled to prepare a palatinose syrup.

상기 제 1 혼합액에 동결 건조된 락토바실러스 플란타룸(Lactobacillus plantarum) 비피도박테리움(Bifidobacterium ), 락토바실러스 불가리커스(Lactobacillus bularicus) 혼합 분말 유산균 0.005 중량% 를 접종하여 40℃ 온도에서 4시간 동안 교반하여 발효시켰다. Wherein the freeze-Lactobacillus Planta room (Lactobacillus plantarum) drying the first mixture Bifidobacterium (Bifidobacterium), Lactobacillus Bulgaria carcass (Lactobacillus bularicus ) mixed powder was inoculated with 0.005% by weight of lactic acid bacteria and fermented by stirring at 40 ℃ temperature for 4 hours.

유산균 발효액의 적정산도(titratable acidity)가 0.75%에 도달하면 팔라티노스 시럽을 혼합하여 100 내지 300 kg/cm2의 압력으로 교반시키면서 균질화하여 실시예 1의 쌀 음료 조성물을 제조하였다.
When the titratable acidity of the lactic acid bacteria fermentation broth reached 0.75%, palatinose syrup was mixed and homogenized while stirring at a pressure of 100 to 300 kg / cm 2 to prepare a rice beverage composition of Example 1.

당도계(RX-5000α, ATAGO)를 이용하여 상기에서 얻어진 실시예 1의 당도를 측정한 결과, 당도가 13.4 ∼ 13.8 브릭스(brix)정도로 적절한 것으로 나타났다. pH meter(Thermo Orion model 550A)를 이용하여 상기에서 얻어진 제품의 pH와 산도를 측정한 결과, 산도 0.70 내지 0.75%, pH 4.2 내지 4.4 정도로 적절한 산미를 나타내었다.
As a result of measuring the sugar content of Example 1 obtained above using a sugar meter (RX-5000α, ATAGO), it was found that the sugar content was about 13.4 to 13.8 brixes. The pH and acidity of the product obtained above were measured using a pH meter (Thermo Orion model 550A), and the acidity was 0.70 to 0.75% and pH 4.2 to 4.4.

비교예Comparative example 1 One

실시예 1의 쌀 음료 조성물에서 팔라티노스 4.0 중량% 대신 감미와 진한 맛을 높이기 위해 설탕 1.0 중량%, 액상과당 2.0 중량%를 첨가하였으며, 락토바실러스 플란타룸(Lactobacillus plantarum), 비피도박테리움(Bifidobacterium), 락토바실러스 불가리커스(Lactobacillus bularicus)의 혼합 분말 유산균을 대신하여 비피도박테리움 아니말리스(Bifidobacterium . animalis), 락토바실러스 파라사실(Lactobacillus paracasei)를 첨가하는 것을 제외하고는 동일한 방법으로 비교예 1의 쌀 음료 조성물을 제조하였다. 상기 실시예 1의 쌀 음료 조성물과 비교하기 위하여, 당도 13.4 ∼ 13.8 브릭스, 산도 0.70 내지 0.75%, pH 4.2 내지 4.4 정도로 맞추어 제조하였다.
In the rice beverage composition of Example 1, 1.0% by weight of sugar and 2.0% by weight of liquid fructose were added to enhance sweetness and rich taste instead of 4.0% by weight of palatinose, and Lactobacillus plantarum ( Lactobacillus) plantarum), Bifidobacterium (Bifidobacterium), Lactobacillus Bulgaria and Caicos (on behalf of the mixed powder of lactic acid bacteria of Lactobacillus bularicus) the addition of Bifidobacterium No Marlies (Bifidobacterium. animalis), Lactobacillus para fact (Lactobacillus paracasei) A rice beverage composition of Comparative Example 1 was prepared in the same manner except. In order to compare with the rice beverage composition of Example 1, the sugar content was prepared according to about 13.4 to 13.8 brix, acidity 0.70 to 0.75%, pH 4.2 to 4.4.

시험예Test Example 1 : 관능 평가 및  1: sensory evaluation and 유산균수Lactobacillus 측정 시험 Measurement test

상기 제조한 실시예 1 및 비교예 1에 대하여 차이식별 능력이 있는 내부 관능 요원 15명을 선정하여, 향, 맛, 색상 및 종합 기호도에 대한 관능 검사를 5점 척도법(1. 매우 나쁘다, 2. 나쁘다, 3. 보통이다, 4. 좋다, 5. 매우 좋다)으로 관능평가를 실시하였다. 그 결과를 표 1에 기재하였다.
15 internal sensory agents with difference discrimination ability were selected for Example 1 and Comparative Example 1 prepared above, and the sensory test for aroma, taste, color, and general preference was performed by a 5-point scale method (1. Bad, 3. normal, 4. good, 5. very good). The results are shown in Table 1.

규격기준Standard 0일0 days 7일7 days 14일14 days 21일21st 31일31st 실시예
1
Example
One
비교예
1
Comparative Example
One
실시예
1
Example
One
비교예
1
Comparative Example
One
실시예
1
Example
One
비교예
1
Comparative Example
One
실시예
1
Example
One
비교예
1
Comparative Example
One
실시예
1
Example
One
비교예 1Comparative Example 1
당도
(Brix)
Sugar content
(Brix)
13.613.6 13.513.5 13.613.6 13.413.4 13.713.7 13.513.5 13.513.5 13.713.7 13.613.6 13.513.5 13.713.7
산도
(w/w%)
Acidity
(w / w%)
0.70.7 0.70.7 0.70.7 0.70.7 0.70.7 0.70.7 0.70.7 0.70.7 0.70.7 0.70.7 0.70.7
pHpH 4.34.3 4.324.32 4.344.34 4.284.28 4.334.33 4.264.26 4.394.39 4.394.39 4.34.3 4.34.3 4.394.39 이물/성상Foreign body / appearance No No No No No No No No No No No 유산균(cfu/ml)Lactobacillus (cfu / ml) 106 10 6 106 10 6 105 10 5 107 10 7 104 10 4 107 10 7 104 10 4 107 10 7 103 10 3 107 10 7 103 10 3 관능평가Sensory evaluation incense 4.14.1 3.93.9 44 44 44 3.83.8 44 3.83.8 3.93.9 3.83.8 flavor 4.34.3 44 4.24.2 44 3.93.9 3.93.9 3.83.8 3.73.7 3.63.6 3.73.7 색상color 4.24.2 4.44.4 4.24.2 4.24.2 44 4.24.2 44 4.34.3 44 4.24.2 종합
기호
Synthesis
sign
Degree
4.24.2 4.14.1 4.14.1 44 3.93.9 44 3.83.8 3.83.8 3.93.9 3.83.8

상기 실시예 1 조성물의 제조 시 관능 평가 결과, 향 4.1, 맛 4.3, 색상 4.2, 종합기호도 4.2의 점수를 받았으며, 관능적으로 우수하다는 것을 확인할 수 있었다. As a result of sensory evaluation during the preparation of the composition of Example 1, the score of 4.1, taste 4.3, color 4.2, the general symbol 4.2 was confirmed, it was confirmed that the sensory excellent.

제조된 쌀 음료에 대해서 10℃ 이하 온도조건에서 4주까지 유산균 생균수를 확인한 결과 107 cfu/ml 이상의 유산균이 생존한 것을 확인함으로써 산업화하기에 적합한 것으로 나타났다. As a result of confirming the viable count of lactic acid bacteria for 4 weeks at the temperature condition below 10 ℃ for the prepared rice beverage, it was found that the lactic acid bacteria of 10 7 cfu / ml or more survived and was suitable for industrialization.

반면, 비교예 1은 실시예 1의 쌀 음료 조성물과 기호도, 맛, 풍미, 색상 등에 있어서 비슷한 것으로 나타났으나, 비교예 1에서 얻어진 쌀 음료는 1주 후 유산균 수가 105 cfu/ml 이하로 유산균 수의 개체가 급격히 감소하여 유산균 음료로 적절하지 않은 문제점을 보였다.
On the other hand, Comparative Example 1 was found to be similar to the rice beverage composition of Example 1 in preference, taste, flavor, color, etc., but the rice beverage obtained in Comparative Example 1 lactic acid bacteria was less than 10 5 cfu / ml after one week The number of subjects declined drastically, and the lactic acid bacteria beverage was not suitable.

이상의 결과로부터, 설탕과 액상과당을 포함하지 않고, 팔라티노스를 포함하고 있는 본 발명에 따른 실시예 1의 쌀 음료 조성물은 비교예 1의 쌀 음료 조성물에 비하여 기호도, 맛, 풍미, 색상 등에 있어서 차이가 없어 충분히 대체하여 사용하여도 산업화하기에 적합하며, 락토바실러스 플란타룸(Lactobacillus plantarum), 비피도박테리움(Bifidobacterium), 락토바실러스 불가리커스(Lactobacillus bularicus)의 쌀 베이스 발효시 유산균의 생육 활성이 우수하였다.From the above results, the rice beverage composition of Example 1 according to the present invention, which does not contain sugar and liquid fructose and contains palatinose, differs in preference, taste, flavor, color, etc., compared to the rice beverage composition of Comparative Example 1. It is suitable for industrialization even if it is sufficiently substituted and it is not suitable for the industrialization. Excellent.

Claims (9)

동결건조된 락토바실러스 플란타룸(Lactobacillus plantarum), 비피도박테리움(Bifidobacterium), 락토바실러스 불가리커스(Lactobacillus bularicus) 혼합 분말 유산균; 쌀 농축액; 우유; 탈지분유; 팔라티노스; 펙틴; 구연산; 식이섬유 및 정제수를 포함하는 것을 특징으로, 기능성 당을 함유하는 쌀 음료 조성물.Freeze-dried Lactobacillus plantarum plantarum), Bifidobacterium (Bifidobacterium), Lactobacillus Bulgaria and Caicos (Lactobacillus bularicus ) mixed powder lactic acid bacteria; Rice concentrate; milk; Skim milk powder; Palatinose; pectin; Citric acid; A rice beverage composition containing a functional sugar, comprising dietary fiber and purified water. 청구항 1에 있어서, 상기 쌀 음료 조성물은 조성물 총 중량에 대하여 동결건조된 락토바실러스 플란타룸(Lactobacillus plantarum), 비피도박테리움(Bifidobacterium), 락토바실러스 불가리커스(Lactobacillus bularicus) 혼합 분말 유산균 0.005 내지 0.1 중량%; 쌀 농축액 8 내지 15중량%; 우유 15 내지 40 중량%; 탈지분유 1 내지 4 중량%; 팔라티노스 1 내지 5 중량%; 펙틴 0.01 내지 0.3 중량%; 구연산 0.0005 내지 0.1 중량%; 식이섬유 0.1 내지 5 중량%; 및 잔량의 정제수를 포함하는 것을 특징으로, 기능성 당을 함유하는 쌀 음료 조성물.The method of claim 1, wherein the rice beverage composition is Lactobacillus plantarum ( Lactobacillus lyophilized relative to the total weight of the composition plantarum), Bifidobacterium (Bifidobacterium), Lactobacillus Bulgaria and Caicos (Lactobacillus bularicus ) mixed powder lactic acid bacteria 0.005 to 0.1% by weight; Rice concentrate 8 to 15% by weight; 15-40% milk; 1 to 4 wt% skim milk powder; 1-5% by weight of palatinose; 0.01-0.3 wt% pectin; Citric acid 0.0005 to 0.1 wt%; 0.1 to 5% by weight of dietary fiber; And a residual amount of purified water, wherein the rice beverage composition contains a functional sugar. 청구항 1에 있어서, 동결건조된 락토바실러스 플란타룸(Lactobacillus plantarum), 비피도박테리움(Bifidobacterium), 락토바실러스 불가리커스(Lactobacillus bularicus) 혼합 분말 유산균은 락토바실러스 불가리커스(Lactobacillus bularicus), 비피도박테리움(Bifidobacterium), 락토바실러스 비피도박테리움(Bifidobacterium)가 3 ~ 7 : 2 ~ 4 : 0.5 ~ 2 중량 비율로 혼합되는 것을 특징으로 하는, 기능성 당을 함유하는 쌀 음료 조성물.The method according to claim 1, Lactobacillus plantarum ( Lactobacillus plantarum ), Bifidobacterium ( Bifidobacterium ), Lactobacillus Bulgaricus ( Lactobacillus) bularicus mixed powder lactic acid bacteria Lactobacillus bularicus), Bifidobacterium (Bifidobacterium), Lactobacillus Bifidobacterium (Bifidobacterium) is 3-7: rice drink composition containing, functional sugar characterized in that the mixture from 0.5 to 2 weight ratio: 2-4 . 청구항 1에 있어서, 상기 쌀 농축액은
(1) 현미와 백미를 정선한 후 150 ∼ 230 ℃에서 10 ∼ 20 분간 볶는, 볶음 공정 단계;
(2) 상기 (1)단계에서 얻은 현미와 백미를 10 ∼ 50 메쉬(mesh)로 분쇄하여 1:9 내지 2:8 중량비율로 혼합하여, 현미 및 백미 혼합원료에 대하여 4 ∼ 8 배의 정제수를 가한 후 100℃로 가열하면서 교반시키는, 교반 단계;
(3) 상기 (2)단계에서 교반시켜 얻은 재료를 88 ∼ 96℃로 냉각시키고 액화효소인 박테리아 α-아밀라아제(Bacteria α-Amylase)를 상기 재료 100 중량부 대비 0.02 ∼ 0.06 중량부를 첨가하여 10 ∼ 30분간 효소 분해시켜 당도 6 ∼ 11 브릭스(Brix)의 1차 효소 분해액을 얻는, 1차 효소 분해 단계;
(4) 상기 (3)단계에서 얻은 1차 효소 분해액을 다시 50 ∼ 60℃로 냉각한 후, 상기 1차 효소 분해액 100 중량부 대비 당화효소인 글루코아밀라아제(glucoamylase)를 0.05 ∼ 0.25 중량부, 단백질 분해효소인 프로테아제(protease)를 0.1 ∼ 0.3 중량부, 펙틴 분해효소를 0.05 ∼ 0.25 중량부로 첨가하여 3 ∼ 8 시간 효소 분해시켜, 당도 8 ∼ 13 브릭스(Brix)의 2차 효소 분해액을 얻는, 2차 효소 분해 단계;
(5) 상기 (4)단계에서 얻어진 2차 효소 분해액을 100℃로 5 ∼ 10 분간 가열시켜 효소를 실활시키고, 아울러 효소반응을 중지시키는, 효소 실활 단계;및
(6) 상기 (5)단계에서 효소 실활 단계를 거친 2차 효소 분해액을 40 ∼ 120 메쉬(mesh)로 1차 여과 후, 2차 원심 분리하여 잔류물을 제거하여, 당도 11 ∼ 13 브릭스(Brix)의 쌀 농축액을 제조하는 단계를 포함하여, 제조되는 것을 특징으로 하는, 쌀 음료 조성물.
The method of claim 1, wherein the rice concentrate
(1) a roasting step of selecting brown and white rice and then roasting at 150 to 230 ° C. for 10 to 20 minutes;
(2) grinding the brown rice and the white rice obtained in step (1) into 10 to 50 mesh and mixing them at a weight ratio of 1: 9 to 2: 8, and 4 to 8 times purified water with respect to the brown and white rice mixed raw materials. After the addition of the stirring step of stirring while heating to 100 ℃;
(3) Cool the material obtained by stirring in step (2) to 88-96 ° C and add 0.02-0.06 parts by weight of liquefied enzyme bacteria α-Amylase to 100 parts by weight of the material to 10- A first enzymatic digestion step of enzymatic digestion for 30 minutes to obtain a primary enzymatic digestion solution with a sugar content of 6 to 11 Brix;
(4) After cooling the primary enzyme digestion solution obtained in step (3) again to 50-60 ° C., 0.05 to 0.25 parts by weight of glucoamylase, a glycosylating enzyme, relative to 100 parts by weight of the primary enzyme digestion solution. , 0.1 to 0.3 parts by weight of protease, a protease, and 0.05 to 0.25 parts by weight of pectinase, were added for enzyme digestion for 3 to 8 hours, and the second enzyme digestion solution having a sugar content of 8 to 13 Brix was obtained. Secondary enzymatic digestion step;
(5) an enzyme inactivation step of heating the secondary enzyme digestion solution obtained in step (4) at 100 ° C. for 5 to 10 minutes to inactivate the enzyme and stop the enzymatic reaction; and
(6) After the first filtration of the second enzymatic digestion solution, which had undergone the enzyme inactivation step in step (5), with 40 to 120 mesh, the residue was removed by secondary centrifugation to obtain a sugar content of 11 to 13 brix ( A rice beverage composition, comprising the step of preparing a rice concentrate of Brix).
청구항 1에 있어서, 상기 식이섬유는 치커리 식이섬유, 썬화이버 또는 이들의 혼합물인 것을 특징으로 하는 쌀 음료 조성물.The rice beverage composition of claim 1, wherein the dietary fiber is chicory dietary fiber, sunfiber, or a mixture thereof. (A) 쌀 농축액을 제조하는 단계;
(B) 우유 15 내지 40 중량%, 탈지분유 1 내지 4 중량%를 배합한 뒤 상기 (A)단계에서 제조된 쌀 농축액을 8 ~ 15 중량%를 배합탱크에 투입하여 배합하여 교반한 후, 살균 냉각하여 제 1 혼합물을 만드는 단계; 및
(C) 팔라티노스 1 내지 5 중량%, 펙틴 0.01 내지 0.3 중량%, 구연산 0.0005 내지 0.1 중량%, 식이섬유 0.1 내지 5 중량% 및 잔량의 정제수를 배합탱크에 투입하여 배합하여 교반한 후, 살균 냉각하여 팔라티노스 시럽을 제조하는 단계;
(D) (B)단계의 제 1 혼합물에 동결건조된 락토바실러스 플란타룸(Lactobacillus plantarum), 비피도박테리움(Bifidobacterium), 락토바실러스 불가리커스(Lactobacillus bularicus) 혼합 분말 유산균을 접종하여 36 내지 45℃ 온도에서 3 내지 12 시간 동안 교반하여 발효시켜, 유산균 발효액을 제조하는 단계;
(E) 상기 (D) 단계에서 유산균 발효액의 적정 산도(titratable acidity)가 0.75%에 도달하면 상기 (C)에서 제조된 팔라티노스 시럽과 혼합하여 100 내지 300 kg/cm2의 압력으로 교반시키면서 균질화하는 단계;를 포함하는 쌀 음료 제조방법.
(A) preparing a rice concentrate;
(B) 15 to 40% by weight of milk and 1 to 4% by weight of skim milk powder, followed by mixing and stirring 8 to 15% by weight of the rice concentrate prepared in step (A) to the mixing tank, followed by sterilization. Cooling to form a first mixture; And
(C) 1 to 5% by weight of palatinose, 0.01 to 0.3% by weight of pectin, 0.0005 to 0.1% by weight of citric acid, 0.1 to 5% by weight of dietary fiber and the remaining amount of purified water were added to the mixing tank, followed by mixing and stirring, followed by sterilization cooling. Preparing palatinose syrup;
(D) (B) The freeze-dried in the first step a mixture of Lactobacillus Planta room (Lactobacillus plantarum), Bifidobacterium (Bifidobacterium), Lactobacillus Bulgaria and Caicos (Lactobacillus bularicus ) inoculated mixed powder lactic acid bacteria and fermented by stirring for 3 to 12 hours at a temperature of 36 to 45 ℃, to prepare a lactic acid bacteria fermentation broth;
(E) when the titrate (titratable acidity) of the lactic acid bacteria fermentation broth in step (D) reaches 0.75%, it is mixed with palatinose syrup prepared in (C) and homogenized while stirring at a pressure of 100 to 300 kg / cm 2 Rice beverage manufacturing method comprising a.
청구항 6에 있어서, (A) 쌀 농축액을 제조하는 단계는
(1) 현미와 백미를 정선한 후 150 ∼ 230 ℃에서 10 ∼ 20 분간 볶는, 볶음 공정 단계;
(2) 상기 (1)단계에서 얻은 현미와 백미를 10 ∼ 50 메쉬(mesh)로 분쇄하여 1:9 내지 2:8 비율로 혼합하여, 현미 및 백미 혼합원료에 대하여 4 ∼ 8 배의 정제수를 가한 후 100℃로 가열하면서 교반시키는, 교반 단계;
(3) 상기 (2)단계에서 교반시켜 얻은 재료를 88 ∼ 96℃로 냉각시키고 액화효소인 박테리아 α-아밀라아제(Bacteria α-Amylase)를 상기 재료 100 중량부 대비 0.02 ∼ 0.06 중량부를 첨가하여 10 ∼ 30분간 효소 분해시켜 당도 6 ∼ 11 브릭스(Brix)의 1차 효소 분해액을 얻는, 1차 효소 분해 단계;
(4) 상기 (3)단계에서 얻은 1차 효소 분해액을 다시 50 ∼ 60℃로 냉각한 후, 상기 1차 효소 분해액 100 중량부 대비 당화효소인 글루코아밀라아제(glucoamylase)를 0.05 ∼ 0.25 중량부, 단백질 분해효소인 프로테아제(protease)를 0.1 ∼ 0.3 중량부, 펙틴 분해효소를 0.05 ∼ 0.25 중량부로 첨가하여 3 ∼ 8 시간 효소 분해시켜, 당도 8 ∼ 13 브릭스(Brix)의 2차 효소 분해액을 얻는, 2차 효소 분해 단계;
(5) 상기 (4 )단계에서 얻어진 2차 효소 분해액을 100℃로 5 ∼ 10 분간 가열시켜 효소를 실활시키고, 아울러 효소반응을 중지시키는, 효소 실활 단계;및
(6) 상기 (5)단계에서 효소 실활 단계를 거친 2차 효소 분해액을 40 ∼ 120 메쉬(mesh)로 1차 여과 후, 2차 원심 분리하여 잔류물을 제거하여, 당도 11 ∼ 13 브릭스(Brix)의 쌀 농축액을 제조하는 단계를 포함하는 것을 특징으로 하는, 쌀 음료 제조방법.
The method of claim 6, wherein (A) preparing the rice concentrate
(1) a roasting step of selecting brown and white rice and then roasting at 150 to 230 ° C. for 10 to 20 minutes;
(2) grind the brown rice and the white rice obtained in the step (1) into 10 to 50 mesh and mix them at a ratio of 1: 9 to 2: 8, and use 4 to 8 times purified water with respect to the brown and white rice mixed raw materials. A stirring step of adding and stirring while heating to 100 ° C;
(3) Cool the material obtained by stirring in step (2) to 88-96 ° C and add 0.02-0.06 parts by weight of liquefied enzyme bacteria α-Amylase to 100 parts by weight of the material to 10- A first enzymatic digestion step of enzymatic digestion for 30 minutes to obtain a primary enzymatic digestion solution with a sugar content of 6 to 11 Brix;
(4) After cooling the primary enzyme digestion solution obtained in step (3) again to 50-60 ° C., 0.05 to 0.25 parts by weight of glucoamylase, a glycosylating enzyme, relative to 100 parts by weight of the primary enzyme digestion solution. , 0.1 to 0.3 parts by weight of protease, a protease, and 0.05 to 0.25 parts by weight of pectinase, were added for enzyme digestion for 3 to 8 hours, and the second enzyme digestion solution having a sugar content of 8 to 13 Brix was obtained. Secondary enzymatic digestion step;
(5) an enzyme inactivation step of heating the secondary enzyme digestion solution obtained in step (4) at 100 ° C. for 5 to 10 minutes to inactivate the enzyme and stop the enzymatic reaction; and
(6) After the first filtration of the second enzymatic digestion solution, which had undergone the enzyme inactivation step in step (5), with 40 to 120 mesh, the residue was removed by secondary centrifugation to obtain a sugar content of 11 to 13 brix ( Brix) a rice concentrate comprising the step of preparing a concentrate.
청구항 6에 기재된 쌀 음료 제조방법에 의해 제조된 기능성 당 및 유산균을 함유하는 쌀 음료.A rice beverage containing the functional sugar and lactic acid bacteria produced by the rice beverage production method according to claim 6. 청구항 8에 있어서, 상기 쌀 음료는 쌀 요구르트인 것을 특징으로 하는, 기능성 당 및 유산균을 함유하는 쌀 음료.The rice beverage according to claim 8, wherein the rice beverage is rice yogurt.
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