KR20130071645A - Multiplex experiment device for separate temperature controlling - Google Patents

Multiplex experiment device for separate temperature controlling Download PDF

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Publication number
KR20130071645A
KR20130071645A KR1020110138969A KR20110138969A KR20130071645A KR 20130071645 A KR20130071645 A KR 20130071645A KR 1020110138969 A KR1020110138969 A KR 1020110138969A KR 20110138969 A KR20110138969 A KR 20110138969A KR 20130071645 A KR20130071645 A KR 20130071645A
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KR
South Korea
Prior art keywords
sample
temperature
temperature control
independently
heat dissipation
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KR1020110138969A
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Korean (ko)
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이중환
박종필
최성수
이재홍
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케이맥(주)
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Priority to KR1020110138969A priority Critical patent/KR20130071645A/en
Publication of KR20130071645A publication Critical patent/KR20130071645A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/16—Apparatus for enzymology or microbiology containing, or adapted to contain, solid media
    • C12M1/18—Multiple fields or compartments
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C12M1/38—Temperature-responsive control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control

Abstract

The present invention relates to a multi-test apparatus capable of independently temperature control, and more particularly, the present invention includes a sample accommodating part having a plurality of sample accommodating holes, a plurality of thermoelectric elements, a heat dissipating part, and a temperature control part, respectively. A plurality of sample receiving holes correspond to the thermoelectric elements of the respective thermoelectric elements are independently controlled by the temperature control unit, so that a plurality of experiments can be performed simultaneously in one device to increase the economic and time efficiency An independent temperature controllable multi-experiment apparatus that is effective.

Description

Multiplex Experiment Device for separate temperature controlling

The present invention relates to a multi-test apparatus capable of independently temperature control, comprising a sample accommodating part having a plurality of sample accommodating holes, a plurality of thermoelectric elements, a heat dissipation part and a temperature control part, and a plurality of each of the thermoelectric elements. The sample receiving hole corresponds to each of the thermoelectric element is independently controlled by the temperature control unit, and relates to a multi-test apparatus capable of independently temperature control capable of simultaneously performing various experiments in one apparatus.

In the field of biochemistry and molecular biology, most experiments require analysis of samples. Temperature is a very important factor in the analysis of samples, and in sample analysis, the temperature required for the sample needs to be kept constant and must be converted accurately according to a predetermined time. Therefore, in sample analysis experiments in fields such as biochemistry and molecular biology, It can be seen that temperature control is very important.

In addition, different temperatures need to be provided depending on the type of sample, and different temperatures for each type of sample may be characteristics of the sample. Therefore, the control of temperature is also important for experiments to find the optimal temperature for the sample.

An example of an analysis experiment of a sample in which temperature control is important is as follows.

First, in biochemistry, enzyme reactions are performed to find the optimal reaction ratios at different temperatures, which need to be tested at various incubation times and temperatures. Since the experiment takes a long time in the experiment, when one device is provided, the experiment is performed over several days with one device, and thus, there is a possibility that an error occurs in the experimental result due to a difference in external conditions in the experiment.

Second, since molecular biology is a field that mainly examines the hybridization rate and melting of nucleic acids, the change of time and temperature is a very important factor in the molecular biology experiment. .

Third, the temperature change is also an important factor in polymerase chain reaction (PCR) devices that are widely used in the medical field.

A polymerase chain reaction is a reaction for amplifying a desired gene in a specific space by specifically repeating a specific gene, and obtaining a large amount of identical genes using a very small amount of genes.

The polymerase chain reaction is performed by mixing genes of interest with reagents required for polymerase chain reaction such as polymerase, single base, and polymerization reagent, and then heating and cooling to the temperature required for the reaction to allow gene synthesis to be performed. Say that. The polymerase chain reaction is performed by repeating a series of temperature changes through a process such as denaturation, primer binding, and gene polymerization.

In order to obtain a replica gene through the polymerase chain reaction, accurate temperature control is required in which the temperature rises and falls rapidly in accordance with the set temperature.

That is, it is required to move the temperature step instantaneously. If a time delay occurs in the process of changing to a set temperature, the reaction rate is lowered, as well as the overall cycling time (longest part of the PCR technique). In applications, it is desirable to complete a series of temperature cycles in the shortest amount of time), since unnecessary reactions proceed to produce by-products.

For such periodic heating and cooling, various techniques are used in the polymerase chain reaction device (hereinafter, 'PCR device'). In general, heating is performed by using an electric heating means, and cooling is performed using the Peltier effect ( Various methods have been adopted, such as cooling using a peltier effect, forced convection cooling using a blower, and using a typical refrigeration unit.

Finally, temperature control is very important even in tissue culture experiments. This is because in the tissue culture experiment, temperature has a very important effect on the growth of cells. In normal tissue culture experiments, the hatching device uses a fixed temperature, but the cells of various types are cultured at a temperature slightly different from each other, so the optimum temperature for each cell can be cultured. You need to provide However, in order to satisfy all of the optimum incubation temperature of various cells with a single experimental device, a number of experiments have to be carried out, so there is a problem that it takes a huge amount of time.

Therefore, as described above, experiments for analyzing samples have been used in various fields, which can be seen that the control of temperature is very important. However, conventionally, various samples were sequentially analyzed in order to perform various experiments in one apparatus. This method reduces the efficiency of the experiment by inserting and ejecting several samples several times, and increases the error rate because the external environment changes due to the repeated experiments.

In addition, when a plurality of experiments are conducted in one engine, the plurality of experiments require different temperature control. In the conventional temperature controllable experimental apparatus, since only one experiment is possible because one temperature is set in one apparatus, there is a problem in that a large amount of equipment is required and it is not economically efficient in time.

1 is a perspective view showing a conventional temperature controllable experiment apparatus 10, the conventional temperature controllable experiment apparatus 10 is a sample accommodating part 1 is formed with a plurality of sample accommodating holes (1 ') for receiving a sample And, the thermoelectric element (2) coupled to the lower portion of the sample accommodating part (1); And a heat dissipation unit (3) including at least one heat sink connected to the thermoelectric element (2); and the thermoelectric element temperature A temperature control unit 4 for controlling the; And it characterized in that it comprises a sample detecting unit (5) for detecting a sample contained in the sample receiving unit (1).

The conventional temperature controllable experimental apparatus 10 is characterized by using a Peltier thermoelectric element as the thermoelectric element 2, and the Peltier thermoelectric element is commercially available in various sizes. In addition, the sample accommodating part (1) proposes a standard by the need for automation and international compatibility of the production in the Society for Biomolecular Sciences (SBS), the sample receiving hole (1 ') of The number is 96, 384 and 768. In addition, the sample accommodating part 1 of high density is defined to have 1536, 3456, 9600 sample accommodating holes 1 '.

The conventional temperature controllable experimental apparatus 10 has at least one thermoelectric element 2, but the sample accommodating part 1 conforms to international standards, so that the area of the thermoelectric element 2 and the sample accommodating state are provided. There is a problem that the areas of the section 1 do not coincide. Therefore, the conventional temperature controllable experimental apparatus 10 has a quantity of heat transferred to the sample receiving hole 1 'located at the edge portion and the portion of the plurality of sample receiving holes 1' that are in contact with the thermoelectric element 2. There may be a difference in that the temperature can be uneven.

In addition, since the Peltier thermoelectric element is marketed in various sizes, even if the thermoelectric element 2 produced in the same area as the sample accommodating part 1 is used, the thermoelectric element 2 has a temperature for one experiment. Because it is controlled only by the system, various experiments cannot be performed at the same time.

In addition, when the temperature controllable experimental apparatus 10 is used as a PCR apparatus, if the sample is not prepared as many as the number of the sample accommodating holes 1 'defined by the standard specification of the sample accommodating unit, the sample may be driven even if the PCR apparatus is driven. Since the number of samples is shorter than the number of the accommodating holes 1 ', there is an inefficient problem in terms of energy efficiency.

On the other hand, Korean Patent Publication No. 2011-0054738 (hereinafter referred to as "PCR device" prior art 1) is to amplify the DNA amplification unit is arranged in a plurality, and each corresponding to the amplification unit is connected between a plurality of the amplification unit When the DNA amplification is performed, a PCR device including a heat exchanger for heating or cooling a plurality of the amplification units and exchanging heat between the plurality of amplification units has been disclosed.

Prior art 1 has the effect of reducing the amount of power consumed in the amplification unit and the heating and cooling performance of the amplification unit can be improved, but the problem that the temperature non-uniformity and a plurality of experiments cannot be performed at the same time cannot be solved.

Therefore, there is a need for an experimental apparatus capable of independently controlling temperature in one apparatus so that a plurality of experiments may be simultaneously performed to increase economic and time efficiency.

Korean Patent Publication No. 2011-0054738 (Registration Publication Date 2011.05.25)

The present invention has been made to solve the above problems, an object of the present invention is to have a plurality of thermoelectric elements, by allowing each of the thermoelectric elements to be independently temperature control, a plurality of experiments in one device It is an object of the present invention to provide an independent temperature-controlled multi-experiment apparatus that can be made at the same time, thereby increasing economic and time efficiency.

Independently temperature controlled multiple experiment apparatus 1000 of the present invention for achieving the above object is a sample accommodating part 100 is formed with a plurality of sample accommodating holes 110 for receiving a sample; and the sample accommodating part ( 100 is coupled to the lower side, a plurality of thermoelectric elements 200 provided to correspond to the at least one or more sample receiving holes 110; and at least one heat sink 310 connected to the thermoelectric element 200; A heat dissipation unit 300; And a temperature controller 500 for independently controlling the temperatures of the plurality of thermoelectric elements 200.

In another embodiment of the present invention, an independent temperature-controlled multi-test apparatus 1000 includes a sample accommodating part 100 having a plurality of sample accommodating holes 110 for accommodating a sample; and below the sample accommodating part 100. A plurality of thermoelectric elements 200 coupled to each other and provided to correspond to at least one or more of the sample accommodating holes 110; and at least one heat dissipation plate 310 connected to the thermoelectric elements 200 so as to be thermally conductive. Part 300; And a temperature controller 500 for independently controlling the temperatures of the plurality of thermoelectric elements 200. The multi-experimental apparatus 1000 capable of independently controlling temperature includes a region corresponding to one thermoelectric element 200. It constitutes one unit module, characterized in that a plurality of modules can be assembled.

In addition, the independent temperature control multiple experiment apparatus 1000 is an independent temperature control multiple experiment apparatus, characterized in that the assembly with a heat conduction prevention space, when a plurality of modules are assembled.

In addition, the independent temperature-controlled multi-test apparatus 1000 is characterized in that the insulating portion 210 is provided between each of the thermoelectric elements 200 in order to prevent heat transfer between the thermoelectric elements 200. .

In addition, the heat dissipation plate 310 is disposed in a direction perpendicular to the sample accommodating part 100 or in a direction parallel to the sample accommodating part 100.

In addition, the independent multiple temperature control apparatus 1000 is characterized in that it further comprises a heat dissipation fan 400 to help the heat dissipation of the heat dissipation unit 300 on one side of the heat dissipation unit 300.

In addition, the independent multiple temperature control apparatus 1000 is characterized in that it further comprises a heat transfer aid 330 for connecting the thermoelectric element 200 and the heat dissipation unit 300 to each other thermally conducting.

In addition, the independent temperature-controlled multi-test apparatus 1000 is characterized in that the weight reduction unit 120 is formed to reduce the weight of the sample receiving portion 100 by forming a space portion on the sample receiving portion 100. It is done.

In addition, the independent multiple temperature control apparatus 1000 may further include a sample detecting unit 600 for detecting a sample contained in the sample receiving unit 100, the sample detecting unit 600, respectively Characterized in that it independently detects the sample accommodated in the plurality of sample receiving holes 110 corresponding to the thermoelectric element of the.

In addition, the temperature controller 500 is characterized in that each of the thermoelectric elements 200 can be independently controlled by using the H-bridge circuit.

In addition, the independent temperature-controlled multi-test apparatus 1000 is characterized in that the region corresponding to one of the thermoelectric element 200 forms one unit module, a plurality of modules can be assembled.

Accordingly, an independent temperature controllable multi-test apparatus of the present invention includes a plurality of thermoelectric elements, and each of the thermoelectric elements is independently controlled by a temperature controller, so that a plurality of experiments can be simultaneously performed in one apparatus. There is an effect that can increase the economic and time efficiency.

In addition, the independent temperature-controlled multi-experiment apparatus is independently controlled the temperature of each thermoelectric element, so that each area controlled by the temperature control unit is narrow so that temperature nonuniformity does not occur can maintain a controlled temperature It works.

In addition, the independent temperature-controlled multi-test apparatus can control only the number of samples because each of the thermoelectric elements are independently temperature controlled when the number of samples is less than the sample receiving hole of the sample accommodating portion, There is an effect that can increase the energy efficiency.

1 is a perspective view showing a conventional apparatus capable of temperature control.
Figure 2 is a perspective view showing an independent temperature control multiple experiment apparatus of the present inventors.
Figure 3 is an exploded perspective view showing an independent temperature control multiple experimental apparatus of the present invention.
4 is a perspective view showing an independent temperature controllable multiple experimental apparatus of the present invention having a sample accommodating part of a different shape.
Figure 5 is a perspective view showing another embodiment of the present inventors independent temperature control multiple experiment apparatus.
Figure 6 is a cross-sectional view of the inventors independently temperature control multiple experiment apparatus.
7 is a cross-sectional view of an independent temperature controllable multiple experimental apparatus of the present invention further comprising a heat transfer aid.
Figure 8 is a cross-sectional view showing an independent temperature control multiple experimental apparatus of the present invention further comprises a heat radiating fan.
9 is a cross-sectional view of an independent temperature controllable multi-experiment apparatus of the present invention further including heat transfer aid of another embodiment.
10 is a perspective view showing an independent temperature controllable multiple experiment apparatus of the present invention having a weight reduction portion formed in the sample accommodating portion.
Figure 11 is a schematic diagram showing the operation of the temperature control unit of the present invention independently temperature control multiple experimental apparatus.

Hereinafter, an independent temperature controllable multi-experiment apparatus of the present invention having the features as described above will be described in detail with reference to the accompanying drawings.

FIG. 2 is a perspective view illustrating an independent temperature control multiple experiment apparatus 1000 according to the present invention, and FIG. 3 is an exploded perspective view illustrating an independent temperature control multiple experiment apparatus 1000 according to the present invention. Referring to Figures 2 and 3 will be described in detail the present invention independent temperature control multiple experiment apparatus 1000.

Independently temperature controlled multiple experiment apparatus 1000 of the present invention includes a sample receiving portion 100 is formed with a plurality of sample receiving holes 110 for receiving a sample; and is coupled to the lower side of the sample receiving portion 100, A heat dissipation part 300 including a plurality of thermoelectric elements 200 corresponding to the plurality of sample accommodating holes 110 and at least one heat dissipation plate 310 connected to the thermoelectric elements 200 so as to conduct heat conduction; And a temperature controller 500 for independently controlling the temperatures of the plurality of thermoelectric elements 200.

The sample accommodating part 100 presents a standard specification by the necessity of production automation and international compatibility in the Society for Biomolecular Sciences (SBS), the number of the sample accommodating holes 110 is 96 , 384 and 768. In addition, the high-density sample accommodating part 100 is defined to have 1536, 3456, 9600 sample accommodating holes 110, and the sample accommodating part with 96 of the sample accommodating holes 110 is shown in FIGS. 100 is shown.

The thermoelectric element 200 may be a Peltier thermoelectric element 200. The Peltier effect is a phenomenon in which two types of metal ends are connected and a current flows therethrough, whereby one terminal absorbs heat and the other terminal generates heat according to the current direction. By using a semiconductor such as bismuth and tellurium having different electric conduction methods instead of two kinds of metals, the Peltier thermoelectric element 200 having an efficient endothermic and heat generating effect can be obtained. The Peltier thermoelectric element 200 is capable of switching the endothermic heat generation in accordance with the current direction, and the endothermic heat generation amount is adjusted according to the current amount, so that the Peltier thermoelectric element 200 can be easily used in a temperature control device.

In addition, the thermoelectric element 200 is not limited to the Peltier thermoelectric element 200, and may be modified without departing from the object of the present invention.

In addition, as shown in FIG. 3, the present invention independently temperature-controlled multi-test apparatus 1000 may have six thermoelectric elements 200, which are the area of the sample accommodating part 100 and the thermoelectric element ( Since it is possible to match the area of the 200, there is an advantage that can be made efficient by the uniform heat transfer to the sample receiving portion (100).

In addition, the thermoelectric element 200 is disposed such that the area of the sample accommodating part 100 and the area of the thermoelectric element 200 coincide with each other, and the number of the thermoelectric elements 200 varies without departing from the object of the present invention. It can be characterized in that it can be modified.

4 is a perspective view showing an independent temperature controllable multiple experiment apparatus 1000 of the present invention having the sample accommodating part 100 of another shape. 2 and 3, the sample accommodating part 100 is shown in a plate shape, but the sample accommodating part 100 shown in Fig. 4 is illustrated in a polygonal shape, and the temperature control of the present invention is possible. Experiment apparatus 1000 is characterized in that the sample accommodating part 100 can be variously modified without departing from the object of the present invention as well as a plate shape, polygonal shape.

FIG. 5 is a perspective view showing another embodiment of an independent temperature controllable multi-test apparatus 1000 'of the present invention. In another embodiment of the present invention, an independent temperature controllable multi-test apparatus 1000' is provided for receiving a sample. A sample accommodating part 100 ′ having a plurality of sample accommodating holes 110 ′; and coupled to a lower side of the sample accommodating part 100 ′ and provided to correspond to at least one or more sample accommodating holes 110 ′. A heat dissipation part 300 ′ including a plurality of thermoelectric elements 200 ′ and at least one heat dissipation plate 310 ′ connected to the thermoelectric element 200 ′ so as to conduct heat conduction; And a temperature controller 500 'for independently controlling the temperatures of the plurality of thermoelectric elements 200', wherein the multiple temperature control apparatus 1000 'capable of independently controlling the temperature is one of the thermoelectric elements 200'. A region corresponding to the one unit module, characterized in that the plurality of modules can be assembled.

That is, the one thermoelectric element and the sample accommodating part 100 ', the insulating part 200', the heat radiating part 300 ', the heat radiating fan 400', the temperature control part 500 'and the An area corresponding to the one thermoelectric element 200 'in the sample detecting unit 600' forms one unit module.

FIG. 5 shows that the thermoelectric element 200 'is assembled with six unit modules corresponding to the sixteen sample receiving holes 110' in the independent temperature-controlled multi-test apparatus 1000 'according to another embodiment. It is shown.

Therefore, the independent temperature-controlled multi-test apparatus 1000 ′ may configure a device having various numbers of sample receiving holes 110 ′, such as 16, 32, 48, 64, 96, depending on the number of modules connected thereto. have.

In addition, the independent temperature-controlled multi-test apparatus 1000 is assembled with a heat conduction preventing space when a plurality of modules are assembled to prevent each module from being thermally conductive to each other.

The independent temperature control multiple experiment apparatus 1000 is characterized in that the insulating portion 210 is provided between each of the thermoelectric elements 200, in order to prevent heat transfer between the thermoelectric elements 200.

In addition, the insulation unit 210 prevents heat transfer between each of the thermoelectric elements 200, so that the plurality of sample accommodating holes 110 corresponding to the thermoelectric elements 200 form an independent zone. There is an advantage that the temperature of each thermoelectric element 200 can be controlled independently.

In addition, the insulation portion 210 may be formed in a solid shape or a film using a material such as polystyrene, polypropylene, polyethylene, polyvinyl chloride, nylon, or the like, which are resins. Synthetic resin, which is formed in a shape and belongs to the resin system mainly used for the insulating part 210, is easy to process, and has applications such as corrosion resistance, weather resistance, water resistance, durability, and the like.

In addition, the insulating part 210 may be formed of a material belonging to an inorganic, magnetic, glass, fibrous, rubber, varnish, etc. as well as a material belonging to the resin, and may be modified without departing from the object of the present invention. .

The heat dissipation unit 300 includes at least one heat dissipation plate 310, and the heat dissipation plate 310 is connected to the thermoelectric element 200 to assist cooling of the thermoelectric element 200, thereby allowing the sample accommodating portion 100 to be cooled. ) Facilitates temperature control, and is disposed in a direction perpendicular to the sample accommodating part 100 or in a direction parallel to the sample accommodating part 100.

6 is a cross-sectional view of an independent temperature control multiple experiment apparatus 1000 of the present invention. FIG. 6 (a) shows that the heat sink 310 is disposed in a direction perpendicular to the sample accommodating part 100. 6 is a cross-sectional view of a multi-test apparatus 1000 capable of temperature control, and FIG. 6 (b) shows the independent temperature-controlled multi-test apparatus in which the heat sink 310 is disposed in a direction parallel to the sample accommodating part 100. A cross-sectional view of 1000 is shown.

In addition, the heat dissipation plate 310 is disposed in connection with the thermoelectric element. When a plurality of heat dissipation plates are disposed, the heat dissipation plates 310 are spaced apart from each other by a predetermined interval. It is supported by the case.

In addition, the structure for supporting a plurality of the heat sink 310 is characterized in that the modification can be carried out without change in the object of the present invention in addition to the support frame or case.

The independent multiple temperature control apparatus 1000 is characterized in that it further comprises a heat dissipation fan 400 to help the heat dissipation of the heat dissipation unit 300 on one side of the heat dissipation unit 300. That is, the heat dissipation fan 400 is coupled to the other side in which the plurality of thermoelectric elements 200 are not connected to the heat dissipation unit 300.

The independent multiple temperature control apparatus 1000 is characterized in that it further comprises a heat transfer assisting means 330 for connecting the thermoelectric element 200 and the heat dissipation unit 300 to each other heat conduction.

FIG. 7 is a cross-sectional view of an independent temperature controllable multi-test apparatus 1000 including the heat transfer aid 330. The heat transfer aid 330 is perpendicular to the thermoelectric element 200. It shows what is provided.

FIG. 7 (a) shows that the heat transfer assisting means 330 is the thermoelectric device in the independent temperature controllable multi-test apparatus 1000 in which the heat sink 310 is disposed in a direction perpendicular to the sample accommodating part 100. 7 (b) shows that the heat dissipation plate 310 is arranged independently in the direction parallel to the sample accommodating part 100. The heat transfer assisting means 330 is provided in a direction perpendicular to the thermoelectric element 200.

FIG. 8 is a cross-sectional view illustrating an independent temperature controllable multi-experiment apparatus 1000 including the heat dissipation fan 400, and the heat dissipation fan 400 further includes the heat dissipation fan 400. The multi-test apparatus 1000 capable of temperature control will now be described.

In addition, the heat dissipation fan 400 is to help the heat dissipation of the heat dissipation unit 300, as shown in Figure 8 (a), the heat dissipation plate 310 of the heat dissipation unit 300 and the sample accommodating part 100 When disposed in a vertical direction, the heat dissipation fan 400 is coupled to the lower side of the heat dissipation plate 310 in a direction parallel to the sample accommodating part 100, thereby to provide high temperature air inside the heat dissipation part 300. It acts to blow outside.

That is, the plurality of thermoelectric elements 200 are coupled to the lower side of the sample accommodating part 100, and the heat sink 310 is connected to the thermoelectric element 200 to be perpendicular to the sample accommodating part 100. At least one spaced apart from each other, the heat dissipation fan 400 is characterized in that coupled to the lower side of the heat dissipation plate 310 in a direction parallel to the sample receiving portion (100).

In addition, as shown in Figure 8 (b), when the heat dissipation plate 310 of the heat dissipation unit 300 is disposed in a direction parallel to the sample accommodating portion 100, the heat dissipation fan 400 is the sample accommodating portion ( It is coupled to the side adjacent to the sample accommodating portion 100 in the heat dissipation plate 310 in the direction perpendicular to 100, and serves to blow high temperature air inside the heat dissipation unit 300 to the outside.

That is, the plurality of thermoelectric elements 200 are coupled to the lower side of the sample accommodating part 100, and the heat sink 310 is connected to the thermoelectric element 200 to be parallel to the sample accommodating part 100. At least one spaced apart from each other, and the heat dissipation fan 400 is coupled to the sample accommodating part 100 in a direction perpendicular to the sample accommodating part 100 at the heat dissipating plate 310. do.

FIG. 9 is a cross-sectional view of an independent temperature controllable multi-test apparatus 1000 including the heat transfer aid 330 of another embodiment, wherein the heat transfer aid 330 is parallel to the thermoelectric element 200. It shows that provided in one direction.

In addition, the independent multiple temperature control apparatus 1000 is characterized in that it comprises a heat pipe as the heat transfer aid 330.

In addition, the heat transfer aid 330 is characterized in that formed of a material capable of heat transfer, such as aluminum, gold, silver, copper.

In addition, the heat transfer assisting means 330 is not limited to the heat pipe shape, it is characterized in that it can be modified without departing from the object of the present invention.

In addition, the heat transfer auxiliary means 330 is not only provided in the direction perpendicular to or parallel to the thermoelectric element 200, it may be provided in various forms without departing from the object of the present invention.

The independent temperature control multiple experiment apparatus 1000 is characterized in that the weight reducing unit 120 is formed to reduce the weight of the sample accommodating part 100 by forming a space on the sample accommodating part 100. .

That is, since the weight reducing unit 120 is formed on the sample accommodating part 100, the weight of heating and cooling of the thermoelectric element 200 is reduced, thereby increasing the efficiency of heating and cooling.

FIG. 10 is a perspective view showing that the weight reducing part 120 is formed on the sample accommodating part 100 of the independent multiple temperature control apparatus 1000.

As shown in FIG. 10, the weight reducing unit 120 may be formed in a space between the sample accommodating holes 110.

In addition, the weight reduction unit 120 is formed on the sample accommodating unit 100 is not limited in position or number, the shape of the weight reduction unit 120 can also be modified in various forms without departing from the object of the present invention It is characterized by.

In addition, the weight reduction unit 120 may be formed in a groove shape, it is characterized in that it can be modified without departing from the object of the present invention in a variety of forms, such as a groove shape as well as a through shape.

The independent multiple temperature control apparatus 1000 may further include a sample sensing unit 600 capable of sensing a sample accommodated in the sample accommodating unit 100, and the sample sensing unit 600 is each of the above. Characterized in that it independently detects the sample accommodated in the plurality of sample receiving holes 110 corresponding to the thermoelectric element (200).

In many experiments, a real-time detection function for detecting a reaction of a sample in real time is required to observe from the beginning of the reaction, and the conventional temperature controllable experimental apparatus 1 includes a detector capable of real-time detection.

In this real-time detection function, a method of preparing a mixed sample in which a fluorescent material is mixed with a sample, irradiating light to detect the mixed sample, and analyzing fluorescence emitted according to the irradiated light is widely used.

When the fluorescent material is irradiated with light of a specific wavelength, the light of a wavelength longer than the wavelength of the light source irradiated with the wavelength of the generated fluorescence emits light. Therefore, in order to use the real-time detection function, it is necessary to include a suitable light source for causing the fluorescent material to emit fluorescence and a detector capable of detecting only light corresponding to the wavelength of fluorescence emitted by the fluorescent material.

In general, detectors used to detect a sample include photo diodes (PD) and charge coupled devices (CCD). Most detectors use Charge Coupled Devices (CCD), which allow multiple samples to be detected in a single shot. Therefore, in order for the detector to detect a plurality of samples in one shot, the accuracy of the detector and the uniformity of the plurality of samples due to the light irradiated to the center and the edge must be sufficiently considered.

However, in the apparatus 10 for controlling temperature in the related art, since the sensing unit 5 senses the entirety of the sample accommodating part 1 in real time, the sensing part 5 is a predetermined distance from the sample accommodating part 1. Should have Therefore, the conventional temperature controllable experimental apparatus 10 has a disadvantage in that it is difficult to miniaturize the apparatus and the accuracy is also low.

In the independent temperature-controlled multi-test apparatus 1000 of the present invention, a plurality of sample receiving holes 110 corresponding to each of the thermoelectric elements 200 are independently sensed in real time by the sample detecting unit 600. It is characterized by. Therefore, since the area detected by the sample detecting unit 600 is narrowed, it is possible to miniaturize the device and increase the accuracy.

The temperature controller 500 is characterized in that each of the thermoelectric elements 200 can be independently controlled by using the H-bridge circuit.

FIG. 11 is a schematic view and a control flow chart showing the operation of the temperature control unit 500 of the present invention independently temperature controlled multiple experiment apparatus 1000, Figure 11 (a) is a plurality of thermoelectric elements 200 is heated And (b) is a control flow chart showing that each of the thermoelectric elements 200 are independently controlled by the temperature control part 500.

As illustrated in FIG. 11, the independent temperature control multiple experiment apparatus 1000 may recognize that each thermoelectric element 200 is independently controlled by the temperature controller 500.

It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

1000: Independently controlled temperature experiment
100: sample receiving portion 110: sample receiving hole
120: weight reduction unit 200: thermoelectric element
210: insulation 300: heat dissipation
310: heat sink 330: heat transfer aid
400: heat radiating fan 500: temperature control unit
600: sample detection unit

Claims (11)

A sample accommodating part 100 having a plurality of sample accommodating holes 110 for accommodating a sample;
A plurality of thermoelectric elements 200 coupled to a lower side of the sample accommodating part 100 and provided to correspond to at least one or more of the sample accommodating holes 110;
A heat dissipation unit 300 including at least one heat dissipation plate 310 connected to the thermoelectric element 200 so as to be thermally conductive ; And
Independently temperature controlled multiple experiment apparatus comprising a; temperature control unit 500 for independently controlling the temperature of the plurality of thermoelectric elements (200). A sample accommodating part 100 'having a plurality of sample accommodating holes 110' accommodating a sample;
A plurality of thermoelectric elements 200 'coupled to a lower side of the sample accommodating part 100' and provided to correspond to at least one or more of the sample accommodating holes 110 ';
A heat dissipation part 300 'including at least one heat dissipation plate 310' connected to the thermoelectric element 200 'to be thermally conductive ; And
And a temperature controller 500 'for independently controlling the temperature of the plurality of thermoelectric elements 200'.
In the independent multi-test apparatus 1000 ', the temperature corresponding to one thermoelectric element 200' forms one unit module, and a plurality of modules can be assembled independently. Controllable multi experiment device. The method according to claim 1 or 2,
The independent multiple temperature control apparatus 1000 can be
In order to prevent heat transfer between the thermoelectric elements, each of the thermoelectric elements 200, the insulation unit 210 is provided, characterized in that the independent multiple temperature control apparatus. The method according to claim 1 or 2,
The heat sink 310 is
Independently temperature controlled multiple experiment apparatus, characterized in that arranged in a direction perpendicular to the sample receiving portion (100). The method according to claim 1 or 2,
The heat sink 310 is
Independently temperature controlled multiple experiment apparatus, characterized in that arranged in a direction parallel to the sample receiving portion (100). The method according to claim 1 or 2,
The independent multiple temperature control apparatus 1000 can be
Independently temperature control multiple experiment apparatus further comprises a heat dissipation fan 400 to help the heat dissipation of the heat dissipation part 300 on one side of the heat dissipation part 300. The method according to claim 1 or 2,
The independent multiple temperature control apparatus 1000 can be
Independently temperature-controlled multiple experiment apparatus further comprises a heat transfer auxiliary means (330) for connecting the thermoelectric element (200) and the heat dissipation part (300) to mutually conduct heat. The method according to claim 1 or 2,
The independent multiple temperature control apparatus 1000 can be
Independently temperature controlled multiple experiment apparatus, characterized in that the weight reduction portion 120 is formed to reduce the weight of the sample receiving portion 100 by forming a space portion on the sample receiving portion (100). The method according to claim 1 or 2,
The independent multiple temperature control apparatus 1000 can be
Further comprising a sample detecting unit 600 for detecting a sample accommodated in the sample receiving unit 100, the sample detecting unit 600 is a plurality of sample receiving corresponding to each of the thermoelectric element 200 Independently temperature controlled multiple experiment apparatus, characterized in that for independently sensing the sample contained in the hole (110). The method according to claim 1 or 2,
The temperature control unit 500
Independently temperature-controlled multiple experiment apparatus, characterized in that each of the thermoelectric elements (200) can be controlled independently by using an H-bridge circuit. The method of claim 2,
The independent multiple temperature control apparatus 1000 can be
When the plurality of modules are assembled, independent temperature control multiple experiment apparatus, characterized in that the assembly with a space to prevent heat conduction.
KR1020110138969A 2011-12-21 2011-12-21 Multiplex experiment device for separate temperature controlling KR20130071645A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020242263A1 (en) 2019-05-31 2020-12-03 Seegene, Inc. Device and method for detecting light
KR20210106027A (en) * 2019-03-18 2021-08-27 주식회사 씨젠 Thermal cycler with sample holder assembly
WO2021201597A1 (en) * 2020-03-31 2021-10-07 Seegene, Inc. Optical signal detection device

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210106027A (en) * 2019-03-18 2021-08-27 주식회사 씨젠 Thermal cycler with sample holder assembly
WO2020242263A1 (en) 2019-05-31 2020-12-03 Seegene, Inc. Device and method for detecting light
EP3977101A4 (en) * 2019-05-31 2023-01-18 Seegene, Inc. Device and method for detecting light
WO2021201597A1 (en) * 2020-03-31 2021-10-07 Seegene, Inc. Optical signal detection device

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