KR20130069914A - Electrophoresis device that has cathode at the middle and anode at the surroundings - Google Patents

Electrophoresis device that has cathode at the middle and anode at the surroundings Download PDF

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Publication number
KR20130069914A
KR20130069914A KR1020110136964A KR20110136964A KR20130069914A KR 20130069914 A KR20130069914 A KR 20130069914A KR 1020110136964 A KR1020110136964 A KR 1020110136964A KR 20110136964 A KR20110136964 A KR 20110136964A KR 20130069914 A KR20130069914 A KR 20130069914A
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KR
South Korea
Prior art keywords
electrophoresis
dna
electric field
anode
cathode
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KR1020110136964A
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Korean (ko)
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김승찬
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김승찬
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Priority to KR1020110136964A priority Critical patent/KR20130069914A/en
Publication of KR20130069914A publication Critical patent/KR20130069914A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/125—Electrophoretic separation

Abstract

Electrophoretic technology is a biotechnology that is widely used for DNA or RNA-DNA hybridization or separation according to molecular weight of proteins. By fixing the position of the electric field accurately in all directions, the position of the band by the size marker can be confirmed more reliably. Conventional linear electrophores have been ambiguous because of the heterogeneity between the size marker and the band if the direction of the electric field is not correct. However, this device can be overcome within statistically significant range.

Description

Electrophoresis device that has cathode at the middle and anode at the surroundings}

The present invention designed the electrophoretic machine in a circle to overcome some of the ambiguities of the existing electrophoresis.

Electrophoretic technology is a biotechnology that is widely used for DNA or RNA-DNA hybridization or separation according to molecular weight of proteins. First, the influence factors on the electrophoresis of DNA are as follows.

<Factors affecting DNA migration during agarose gel electrophoresis>

1) Larger DNA molecules move slowly

2) The higher the concentration of Agarose, the slower the movement.

3) Movement speed varies according to DNA type (structure). In general, supercoiled DNA moves fastest, followed by linear DNA and then open circular DNA.

4) The higher the load voltage, the faster the moving speed.

5) The direction of the electric field also affects the speed of movement.

6) Ethidium bromide decreases DNA migration rate by 15%.

7) The composition and ionic strength of the electrophoretic buffer also affect the electrophoretic rate.

When electrophoresis is performed linearly from above, the direction of the electric field is not constant, which sometimes causes errors. In the present invention, in order to improve this, by forming the direction of the electric field in all directions to prevent the error.

Solution of various errors using circular electrophoresis.

* Preparation: Agarose gel (agaros, 0.5X TBE, Ethium bromide (EtBr)), DNA (or protein) solution, loading dye, electrophoresis device, power supply, 0.5X TBE,

<Experiment Protocol>

Use the same method as conventional electrophoresis, but use a device as shown in FIG. 1 instead of the existing electrophoretic device.

According to the present invention, the position of the band by the size marker can be confirmed more reliably by fixing the position of the electric field accurately in all directions. Conventional linear electrophores have been ambiguous because of the heterogeneity between the size marker and the band if the direction of the electric field is not correct. However, this device can be overcome within statistically significant range.

1 is a circular (donut type) electrophoresis device. No substance has been added yet. The center is the cathode and the periphery is the anode.
2 is a sample is loaded, the appearance of the band according
3 is a size marker is loaded and the appearance of the band according to
Figure 4 is a picture overlapping the appearance of the band according to the image obtained by loading the size marker and the sample. Use this to find out the relatively purified size.

A donut-type electrophoresis device can't do multiple experiments at once, but it can be read accurately, but there is also a method of electrophoresis by layering it to size markers. First of all, DNA is a molecule of-, which moves from cathode to anode when it moves. For this reason, the electrode was designed as shown in the drawing.

Claims (3)

Circular electrophoresis device with a polar pole in the center, and a polar pole in the periphery, and a device for separating biomolecules (DNA, protein, RNA) through the device or a method of reading the size by applying the same. A method or application of overlapping several of these circular gel samples at the same time for electrophoresis or overlapping them to clearly quantify the size. Electrophoresis of samples on a circular gel electrophoresis method to quantify or correct errors caused by conventional electrophoresis and differences caused by two electrophoresis or applications thereof.
KR1020110136964A 2011-12-19 2011-12-19 Electrophoresis device that has cathode at the middle and anode at the surroundings KR20130069914A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020110136964A KR20130069914A (en) 2011-12-19 2011-12-19 Electrophoresis device that has cathode at the middle and anode at the surroundings

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020110136964A KR20130069914A (en) 2011-12-19 2011-12-19 Electrophoresis device that has cathode at the middle and anode at the surroundings

Publications (1)

Publication Number Publication Date
KR20130069914A true KR20130069914A (en) 2013-06-27

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2021502556A (en) * 2017-11-13 2021-01-28 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Equipment for sample analysis using epitaco electrophoresis

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2021502556A (en) * 2017-11-13 2021-01-28 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Equipment for sample analysis using epitaco electrophoresis
US11691141B2 (en) 2017-11-13 2023-07-04 Roche Sequencing Solutions, Inc. Devices for sample analysis using epitachophoresis

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