KR20130054620A - Composition for eliminating formaldehyde using fermented rice bran - Google Patents

Abstract

PURPOSE: A composition for eliminating porous absorbent using fermented rice bran is provided to fundamentally remove formaldehyde in contrast with using a porous adhesive. CONSTITUTION: A composition for eliminating porous absorbent comprises a rice bran-fermented product as an active ingredient which is obtained by mixing carbon source and energy source into the mixture, adding photosynthetic bacteria into the mixture, and fermenting the mixture. The rice bran fermented product is obtained by additionally adding lactic acid bacteria and yeast into the mixture, together with the photosynthetic bacteria. A removing method of formaldehyde comprises a step of contacting a composition for removing formaldehyde with the formaldehyde. [Reference numerals] (AA) Remaining formaldehyde(mg/kg)

Description

Composition for eliminating formaldehyde using rice bran fermentation {Composition for Eliminating Formaldehyde Using Fermented Rice Bran}

The present invention relates to a composition for removing formaldehyde using rice bran fermentation, and more particularly, to a composition for removing formaldehyde using fermentation by photosynthetic bacteria, yeast and / or lactic acid bacteria of rice bran.

Formaldehyde is a flammable, colorless gas with an irritating odor, and is a highly toxic substance to humans due to its strong protein coagulation action.

Short-term exposure to formaldehyde causes severe irritation to the eyes, nose, and bronchus, causing headaches, dizziness, and tears; prolonged exposure to allergic dermatitis, eczema, cough, sputum, asthma, chronic bronchitis, low birth weight, pregnancy It is known to cause addiction.

Formaldehyde is used as a disinfectant or preservative, and is used for manufacturing leather, photographic plates, explosives, etc., and is widely used for industrial purposes such as coal-based, urea-based or melamine-based synthetic resins. In particular, the main sources of formaldehyde are plywood, foaming agents, insulation, paints, textile fabrics, furniture, sinks, floor heating, combustion of fuels, smoking, household goods, medicines and adhesives.

Formaldehyde removal can be classified into two types: adsorption and chemical reaction.

The technique for removing formaldehyde by adsorption is a technique using a porous adsorbent having a large surface area, and activated carbon, charcoal, activated clay, silica gel, activated alumina, loess and the like are known as such porous adsorbents.

The chemical reaction is a technique of decomposing and removing formaldehyde using a metal oxide-based catalyst such as titanium dioxide or an oxidizing agent such as sodium hydrogen peroxide.

Adsorption is not a technique for fundamentally removing formaldehyde, whereas a chemical reaction is a technique for permanently removing formaldehyde by decomposing formaldehyde.

The present invention discloses a technique capable of decomposing and removing formaldehyde using a fermentation product of rice bran.

An object of the present invention is to provide a composition for removing formaldehyde.

Other and specific objects of the present invention will be presented below.

The inventors of the present invention, as confirmed in the following Examples and Experimental Examples, to prepare a fermentation product of rice bran photosynthetic bacteria and rice bran photosynthetic bacteria, lactic acid bacteria and yeast and the above in a double-woven cotton fabric impregnated with formaldehyde When the fermented product was impregnated and treated, it was confirmed that the amount of residual formaldehyde was significantly reduced compared to the untreated group, and the amount of residual formaldehyde was reduced in a concentration-dependent manner.

These experimental results can be said to have a formaldehyde decomposition effect of the fermentation by photosynthetic bacteria of rice bran.

The present invention is provided on the basis of the experimental results, the formaldehyde removal composition of the present invention is characterized in that it contains photosynthetic bacteria fermentation of rice bran as an active ingredient.

The photosynthetic bacteria fermentation product of rice bran means that obtained by (a) mixing carbon and energy sources in rice bran powder and (b) inoculating the mixture with photosynthetic bacteria.

 Preferably, inoculation of the photosynthetic bacteria of (b) refers to those obtained by further inoculating and fermenting the lactic acid bacteria and yeast with the photosynthetic bacteria.

In the present specification, the "branch rice" is a by-product obtained in the process of refining brown rice with white rice, and refers to the inclusion of rice bran and whistle layer. It should be understood that the rice bran and whistle layer are included in the meaning of the rice bran even if they additionally include the outer skin and / or seed of the outer shell of brown rice and endosperm and / or endosperm.

In addition, in the present specification, the term "active ingredient" means a component that can exhibit activity alone or in combination with a carrier which is itself inactive.

In addition, in the present specification, "photosynthetic bacteria" refers to bacteria that perform carbon assimilation using light energy, such as higher plants. These photosynthetic bacteria are Chlorobium bacteria sp . ), Chromatium sp . ), Rhodospirillum sp . ), Rhodopsendomonas sp . ), Rhodobacter sp . ), Rhodopseudomonas sp . All these photosynthetic bacteria can be used in the present invention, but Rhodo Pseudomonas bacteria or Rhodobacter bacteria are preferred, and Rhododocoseudomonas ( Rhodopseudomonas ) used in the examples below palustris ) or Rhodobacter capsulatus .

In addition, in the present specification, "lactic acid bacteria" refers to microorganisms that produce lactic acid by decomposing sugars such as glucose, and in this specification, lactic acid bacteria are Lactobacillus genus Lactobacillus. sp . ), Streptococcus sp . ), Pediococcus sp . ), Leuconostoc sp . ) And a Bifidobacterium lactic acid bacteria (Bifidobacterium sp . ), Specifically, Lactobacillus exidophilus ( Lactobacillus) acidophilus ), Lactobacillus plantarum ), Lactobacillus fermentum), ronggeom Bifidobacterium (Bifidobacterium longum ), Bifidobacterium bifidum ) and the like. In the present invention, all of these lactic acid bacteria can be used. However, considering the following examples, Lactobacillus genus Lactobacillus is preferred, and Lactobacillus plantarum is particularly preferred.

In the present specification, "yeast" refers to Saccharomyces yeast, Saccharomyces rouxii), saccharose in my process to the celebrity bicyclic (Saccharomyces cereviciae), access to the MY Ob pole miss Saccharomyces (Saccharomyces oviformis ), Saccharomyces steineri ), etc. Preferably Saccaromyces cerevisiae used in the examples below.

In the present specification, "fermented product" means a fermentation stock solution, a concentrated fermentation broth obtained by distillation under reduced pressure, or the like, or a solid fermentation powder obtained by a method such as lyophilization.

Carbon sources and energy sources of microorganisms ie photosynthetic bacteria, lactic acid bacteria and / or yeast inoculated in the preparation of fermentation of rice bran, which are included as an active ingredient in the composition for removing formaldehyde of the present invention, are known in the art and are known in the art. Any may be used, typically molasses, oligosaccharides, lactose, glucose, fructose, sugar, mixtures thereof, and the like. These carbon sources and energy sources may be added in any range in consideration of the intended fermentation time, the degree of fermentation, the amount of rice bran used. It will usually be added in the range of 20 to 120 parts by weight based on 100 parts by weight of rice bran.

In addition, in the preparation of the rice bran fermentation product included as an active ingredient in the composition for removing formaldehyde of the present invention, in addition to the carbon source and the energy source, nitrogen sources, inorganic salts, growth factors, etc. may be further added. , Inorganic salts, growth sites and the like are also known in the art, and any known in the art may be used.

In addition, in the preparation of the rice bran fermentation product included in the composition for removing formaldehyde of the present invention as an active ingredient, the fermentation of (b) is preferably fermented until the growth of the inoculated microorganism reaches a maximum. The point at which the inoculated microorganisms reach their maximum growth will depend on the fermentation temperature, the amount of rice bran used, the amount of carbon and energy sources, and the amount of microorganisms inoculated. One of ordinary skill in the art can determine the point in time at which the growth of the inoculated microorganism reaches its maximum, taking into account the fermentation temperature, the amount of the fermentation raw material, the amount of the microorganism to be inoculated, and the like within its usual capacity. The present inventors fermented for 4 weeks when the inoculated microorganisms were inoculated at 9 wt% with respect to 5 wt% of rice bran and 5 wt% of sugar at a temperature of 30 to 40 ° C. using a sealed fermenter, and the inoculated microorganisms grew to a maximum. It was made.

In addition, in the preparation of fermented rice bran, which is included as an active ingredient in the composition for removing formaldehyde of the present invention, all of the photosynthetic bacteria, lactic acid bacteria and yeast can be cultured under both anaerobic and aerobic conditions, so that the fermentation conditions of (b) It may be anaerobic or aerobic, and may be cancer or photoculture. It is known in the art that photosynthetic bacteria carry out heterotrophs under cancer culture conditions. In the case of the culture temperature can be cultured in the range of 20 ℃ to 45 ℃, the pH of the medium is preferably adjusted to the range of 4.0 to 7.5. However, anaerobic conditions would be desirable given the proliferative properties of lactic acid bacteria.

In addition, in the preparation of fermented rice bran, which is included as an active ingredient in the composition for removing formaldehyde of the present invention, photosynthetic bacteria, lactic acid bacteria and yeast can be purchased and used by a biotechnology company, or domestic and foreign microbial deposit institutions (Biotechnology Research Institute Gene) It can be used by banks, Korea Microorganisms Preservation Center, Korea Agricultural Microorganisms Preservation Center, American Type Culture Collection Asociacin (ATCC), and can be used by directly separating and culturing strains as needed.

The composition for removing formaldehyde of the present invention may include any amount (effective amount) of the rice bran fermentation, an active ingredient thereof, in accordance with its applied product, formulation, purpose of use, and the like as long as it can exhibit the removal activity of formaldehyde. Since the fermentation stock solution can be used as it is, the composition for removing formaldehyde of the present invention may be composed of only fermented products. When using a concentrated fermentation broth or a solid fermentation product, a conventional effective amount is 0.01 weight based on the total weight of the composition. It will be determined in the range of% to 99.91 weight%. As used herein, "effective amount" refers to an amount of an active ingredient capable of exhibiting formaldehyde removal activity, and such an effective amount may be determined experimentally within a range of ordinary skill of those skilled in the art.

Formaldehyde removal composition of the present invention can be impregnated and applied to formaldehyde-generating products, such as furniture, sinks, clothing, car seats, etc., impregnated and applied to plywood, wood, fibers, etc. used in the production of these products Can be used.

It may also be used in addition to formaldehyde-containing adhesives, such as formaldehyde-based adhesives, amino resin-based adhesives, etc., and used for adsorbing porous adsorbents for removing formaldehyde, such as activated carbon, charcoal, activated clay, silica gel, activated alumina, loess, etc. It may be. Furthermore, it may be used in addition to cleaning agents such as clothes, furniture, sinks, and vehicle seats.

In another aspect, the present invention relates to a method of removing formaldehyde.

Formaldehyde removal method of the present invention is characterized in that it comprises the step of contacting the formaldehyde removal composition as described above with formaldehyde.

In the method of the present invention, the contacting step may be carried out by contacting the formaldehyde removing composition with a formaldehyde generating source, which may be, for example, furniture, sink, clothing, vehicle seat, plywood, wood, Fibers, adhesives and the like can be exemplified.

In the method of the present invention, the contacting step may be carried out by impregnating and applying the formaldehyde removal composition directly to the formaldehyde generating source or by adding it to an adhesive, and indirectly added to the cleaning agent or adhered to the porous adsorbent. By using the cleaner or adsorbent as a source of formaldehyde. In addition, considering that formaldehyde is the cause of sick house syndrome, the contacting step may also be performed indirectly by spraying a composition for removing the formaldehyde in the interior of the house, building.

As described above, according to the present invention can provide a composition for removing formaldehyde. Formaldehyde removal composition of the present invention is due to the decomposition activity by the chemical reaction of the rice bran fermentation as its active ingredient and has an effect of removing formaldehyde radically different from the case of using a porous adsorbent.

Formaldehyde removal composition of the present invention can be used in a direct or indirect manner, such as impregnating, applying, adding, spraying the formaldehyde generating source for the purpose of formaldehyde removal.

1 is a result showing the removal activity of formaldehyde of rice bran fermentation.
2 is a result showing that the rice bran fermentation has a removal activity of formaldehyde in a concentration-dependent manner.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

< Example > Rice bran  Photosynthetic bacteria or Rice bran  Caused by photosynthetic bacteria, lactic acid bacteria and yeast Fermented  Produce

Example 1 Fermented product by photosynthetic bacteria in rice bran Production Example 1

The medium was formulated with 81% by weight of water, 5% by weight of rice bran and 5% by weight of sugar, followed by the photosynthetic bacterium Rhodopseudomonas. palustris ) was inoculated at 9% by weight, and cultured in a temperature range of 30 ~ 40 ℃ using a sealed fermenter for 4 weeks to obtain a photosynthetic bacterial fermentation of rice bran. The fermented product was sterilized by irradiation with ultraviolet rays and used for experiments.

Example 2 Fermented product by photosynthetic bacteria in rice bran Production Example 2

The medium was prepared with 81% by weight of water, 5% by weight of rice bran and 5% by weight of sugar, and then inoculated with 9% by weight of photosynthetic bacteria, Rhodobacter capsulatus , and 30-40 with a sealed fermenter for 4 weeks. Incubation was carried out at a temperature range of ℃ to obtain photosynthetic bacterial fermentation of rice bran. The fermented product was sterilized by irradiation with ultraviolet rays and used for experiments.

Example 3 Fermented product by photosynthetic bacteria, lactic acid bacteria and yeast of rice bran Production Example 1

The culture was carried out under the same conditions as in Example 1, except that yeast and lactic acid bacteria were additionally inoculated to photosynthetic bacteria.

Specifically, the medium was prepared with 81% by weight of water, 5% by weight of rice bran and 5% by weight of sugar, and then 5% by weight of photosynthetic bacteria Rhodopseudomonas palustris , Lactobacillus plantarum ( Lactobacillus) plantarum ) 2% by weight, yeast Saccharomyces Saccaromyces cerevisiae ) was inoculated with 2% by weight and cultured at a temperature range of 30-40 ° C. using a sealed fermenter for 4 weeks to obtain fermented products of photosynthetic bacteria, lactic acid bacteria and yeast of rice bran. The fermented product was sterilized by irradiation with ultraviolet rays and then lyophilized and used in experiments. The fermented product was sterilized by irradiation with ultraviolet rays and used for experiments.

Example 4 Fermented product by photosynthetic bacteria, lactic acid bacteria and yeast of rice bran Production Example 2

The culture was carried out under the same conditions as in Example 2, except that yeast and lactic acid bacteria were additionally used in addition to photosynthetic bacteria.

Specifically, the medium was prepared with 81% by weight of water, 5% by weight of rice bran and 5% by weight of sugar, and then the photosynthetic bacteria Rhodobacter ( Rhodobacter) capsulatus ) 5% by weight, Lactobacillus plantarum plantarum ) 2% by weight, yeast Saccharomyces Saccaromyces cerevisiae ) was inoculated with 2% by weight and cultured at a temperature range of 30-40 ° C. using a closed fermenter for 4 weeks to obtain fermented products of photosynthetic bacteria, lactic acid bacteria and yeast of rice bran. The fermented product was sterilized by irradiation with ultraviolet rays and used for experiments.

< Experimental Example > Formaldehyde Removal Activity Measurement

35% formaldehyde was diluted to 5,000 ppm, and the formaldehyde dilution was immersed in a dilution cloth of a width x length of 30 cm for at least 24 hours, and then the soaked double fabric was removed and naturally dried for at least 24 hours. The dried double woven cotton fabric was immersed in the fermentation broth of each Example for 24 hours, then taken out, and naturally dried for at least 24 hours, followed by hot air drying at a low temperature of 30 ° C. in a hot air dryer for 6 hours.

The dried cotton fabric was commissioned by the Korea Institute for Living Environment Testing, and the residual formaldehyde was measured.

The results are shown in [FIG. 1] and [FIG. 2].

1 is a result showing the removal activity of formaldehyde of the rice bran fermentation product (RBF 1 to 4) of each embodiment, [Figure 2] is the highest removal activity of the rice bran fermentation Example of <Example 4> This is the result showing the formaldehyde removal activity of diluent diluted 100 times, 50 times, 10 times, 5 times and 1 times (stock solution) with rice bran fermentation product (RBF4).

Referring to [FIG. 1], it can be seen that the residual formaldehyde content of the rice bran fermentation product of the example is very low compared to the untreated group (Blank). In particular, the formaldehyde removal activity of the fermented products (RBF 3 & 4) of <Examples 3 and 4> obtained by fermenting rice bran with photosynthetic bacteria, yeast and lactic acid bacteria was obtained by fermenting rice bran with photosynthetic bacteria only. Fermentate> (RBF 1 & 2).

In addition, referring to [FIG. 2], it can be seen that the rice bran fermentation product of <Example 4> having the highest formaldehyde removal activity in [FIG. 1] shows the removal activity of formaldehyde in a concentration-dependent manner.

Claims (6)

A composition for removing formaldehyde comprising (a) rice bran mixed with a carbon source and an energy source, and (b) fermented rice bran obtained by inoculating the mixture with photosynthetic bacteria as an active ingredient.
The method of claim 1,
The rice bran fermentation product is a formaldehyde removal composition, characterized in that the fermentation by further inoculating lactic acid bacteria and yeast with the photosynthetic bacteria at the time of inoculation of the photosynthetic bacteria of (b).
The method of claim 1,
The formaldehyde removing composition is a composition for removing formaldehyde, characterized in that the composition for fiber or clothing impregnation.
Formaldehyde removal method comprising the step of contacting the formaldehyde removing composition according to any one of claims 1 to 3 with formaldehyde.
5. The method of claim 4,
The contacting step is characterized in that the formaldehyde removal method is carried out by contacting the formaldehyde removing composition with a formaldehyde source.
The method of claim 5,
The formaldehyde generating method is characterized in that the furniture, sink, clothing, vehicle seat, plywood, wood, fibers or adhesives.

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