KR20130037563A - Compositions containing an extract of machilus thunbergii siebold zuccarini for anti-aging through activation of retinoic acid receptor - Google Patents
Compositions containing an extract of machilus thunbergii siebold zuccarini for anti-aging through activation of retinoic acid receptor Download PDFInfo
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- KR20130037563A KR20130037563A KR1020110102026A KR20110102026A KR20130037563A KR 20130037563 A KR20130037563 A KR 20130037563A KR 1020110102026 A KR1020110102026 A KR 1020110102026A KR 20110102026 A KR20110102026 A KR 20110102026A KR 20130037563 A KR20130037563 A KR 20130037563A
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- extract
- composition
- aging
- retinoic acid
- acid receptor
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- 239000005017 polysaccharide Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
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- 102000004196 processed proteins & peptides Human genes 0.000 description 1
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- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 229940010747 sodium hyaluronate Drugs 0.000 description 1
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 229940031439 squalene Drugs 0.000 description 1
- TUHBEKDERLKLEC-UHFFFAOYSA-N squalene Natural products CC(=CCCC(=CCCC(=CCCC=C(/C)CCC=C(/C)CC=C(C)C)C)C)C TUHBEKDERLKLEC-UHFFFAOYSA-N 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
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- 238000001890 transfection Methods 0.000 description 1
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- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/20—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
- A61K31/203—Retinoic acids ; Salts thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
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- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Dermatology (AREA)
- Birds (AREA)
- Cosmetics (AREA)
Abstract
Description
The present invention relates to a composition for anti-aging through activation of the retinoic acid receptor containing Hanfubak extract as an active ingredient, more specifically Hanhubak to prevent skin aging through the activation of the retinoic acid receptor and inhibiting the expression of collagenase It relates to an anti-aging composition containing the extract as an active ingredient.
As people age, the aging phenomenon gets worse. Especially in the case of the skin, the change is noticeable. The main phenomenon of the skin change is the increase in wrinkles, the skin thickening, elasticity, dryness, roughness and spots, etc. It is interpreted as. This effect of sunlight is called photoaging and is due to changes in epidermis and dermal tissue caused by sunlight. Photoaging of the skin has been reported to be improved by applying a cream to the skin containing tretinoin (all-trans-retinoic acid), retinol and its derivatives, AHAs and the like. However, since tretinoin is fat-soluble, it has low absorption, is unstable in vivo, irritates the skin, and may cause side effects such as skin dryness, wounds, peeling off during incubation periods, and thus it is difficult to use as a main ingredient of medicines and cosmetics. In addition, retinol, which is vitamin A, is difficult to use because it is unstable by light, oxygen, heat, and lipid peroxides, and thus is difficult to use. Accordingly, BHT, di-α-tocopherol, BHA, ascorbic acid, tocopheryl linoleate, etc. The use of antioxidants to help stability or to block the outside to steal the method, such as liposomes or capsules to block the outside, there was a problem that must be invested separately. Therefore, studies on raw materials having side effects and stability while preventing or improving skin aging by activating Retinoic Acid Receptor (RAR) are active, but have excellent skin anti-aging effect and skin stability. The development of excellent raw materials is insignificant.
In order to solve the above problems, the present invention is a composition having the side effects and stability for the skin at the same time to prevent the skin aging by activating the retinoic acid receptor, the natural raw material Hanhubak extract, Hanhubak Seomoktae complex extract and Hanhubak Seoritae An object of the present invention is to provide an external composition for skin containing a complex extract.
In order to solve the above object, the present invention provides a composition for anti-aging containing Hanhubak extract as an active ingredient.
In one embodiment of the present invention, the composition may further contain any one or more of Seomoktae extract and Seoritae extract as an active ingredient.
In one embodiment of the present invention, the extract is selected from the group consisting of anhydrous and hydrous lower alcohol having 1 to 4 carbon atoms, acetone, butylene glycol, ethyl acetate, diethyl acetate, diethyl ether, benzene, chloroform and hexane It may be an extract of any one or more solvents.
In one embodiment of the present invention, the content of the medicinal herb extract may be 0.0001 to 10% by weight based on the total weight of the composition.
In one embodiment of the present invention, the content of the Seomoktae extract may be 0.0001 to 10% by weight based on the total weight of the composition.
In one embodiment of the present invention, the content of the frost extract may be 0.0001 to 10% by weight based on the total weight of the composition.
In one embodiment of the present invention, the composition may be to prevent skin aging through the activation of the retinoic acid receptor, specifically retinoic acid receptor alpha or gamma, luciferase.
In one embodiment of the present invention, the composition may be to prevent skin aging through the inhibition of the expression of collagenase.
The anti-aging composition containing the extract of Hanfubak of the present invention has an excellent anti-aging effect by activating the retinoic acid receptor and suppressing the expression of collagenase without inducing skin side effects. Due to this effect, it can be usefully applied to external skin preparations for the purpose of anti-aging and wrinkle improvement.
Hereinafter, preferred embodiments of the present invention will be described in detail in order to facilitate the present invention by those skilled in the art.
The present invention provides a composition for anti-aging containing Hanhubak extract as an active ingredient.
In addition, the present invention may further contain any one or more of Seomoktae extract and Seoritae extract as an active ingredient, in addition to Hanhubak extract.
Han-bak is a dried bark of Machilus thunbergii Siebold et Zucc. Its features are greyish brown or dark brown on the outside, with light grayish white spots and vertical or horizontal rough wrinkles, and reddish brown inside. Slightly smooth The pharmacological action of Hanhubak is known as hepatoprotective effect, melanin production inhibition, NO synthesis inhibitory action, is used in folk bloating, indigestion, hepatitis, constipation. However, there have been no studies on the antiwrinkle and anti-aging effects of Hanhubak.
Seomoktae (Rhynchosia Nolubilis) is known as medicinal soybeans, rich in protein and vitamins, the glycinein of the components of the anti-cancer substance has an antioxidant effect. Herbal medicine is known to have the effect of suppressing all the wind fever (風 熱), through the clogged veins. In addition, all poisons, including the poison of mineral medicines, and the blood circulation is active.
Glycine max (L.) Merr. Is commonly known as black soybeans, black soybeans or black soybeans, and contains high amounts of glycidane, anthocyanins and isoflavones. Glycidine has anticancer activity, and anthocyanin has the effect of lowering cholesterol and inhibiting arteriosclerosis, and isoflavone is supplemented with insufficient female hormone to maintain feminine body shape and stabilize body temperature and autonomic nerve. It is reported to be effective. In addition, since the antioxidant function of removing free radicals is four times higher than that of ordinary beans, it is known that the frost is also effective in preventing skin aging. In other words, it is confirmed that the effect of improving the function of collagen in the skin to strengthen the elasticity of the skin, give vitality to the skin, and regenerate and heal skin cells.
Hanhubak extract, Seomoktae extract and Seoryae extract of the present invention are dried and pulverized Hanhubak, Seomoktae and Seoritae respectively; The pulverized product obtained above is any one or more extraction solvents selected from the group consisting of anhydrous and hydrous lower alcohols having 1 to 4 carbon atoms, acetone, butylene glycol, ethyl acetate, diethyl acetate, diethyl ether, benzene, chloroform and hexane. Extraction is performed one or more times by cooling or warming; The extract obtained above may be prepared by concentrating under reduced pressure to an appropriate temperature in a distillation apparatus equipped with a cooling condenser.
It is preferable that the Hanhubak extract is contained 0.0001 to 10% by weight based on the total weight of the composition. If the extract of Hanhubak is less than 0.0001% by weight relative to the total weight, the wrinkle improvement and anti-aging effect is insignificant, and when it is more than 10% by weight, there is no obvious increase in the content. Seomoktae extract and seotaetae extract, it is also included for the same reason 0.0001 to 10% by weight based on the total weight of the composition.
The anti-aging composition according to the present invention may be, for example, a pharmaceutical composition or cosmetic composition.
The anti-aging pharmaceutical composition may further contain pharmaceutical supplements such as preservatives, stabilizers, hydrating or emulsifiers, salts and / or buffers for the control of osmotic pressure and other therapeutically useful substances, according to conventional methods. It can be formulated in a variety of parenteral dosage forms. Parenteral dosage forms, and may be, for example, but not limited to lotions, ointments, gels, creams, patches or spray formulations.
Determination of the dosage of the active ingredient is within the level of those skilled in the art, and the daily dosage of the drug depends on various factors such as less progression, onset, age, health condition, complications, etc. of the subject to be administered. Generally, the composition may be administered by dividing 1 μg / kg to 200 mg / kg, preferably 50 μg / kg to 50 mg / kg, once or three times a day, and the dosage may be determined by any method. Nor does it limit the scope of the invention.
The anti-aging cosmetic composition is not particularly limited in formulation, and may be appropriately selected as desired. For example, skin lotion, skin softener, skin toner, astringent, lotion, milky lotion, moisturizing lotion, nutrition lotion, massage cream, nutritional cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, pack, soap, cleansing But the present invention is not limited thereto, and may be manufactured by any one or more formulations selected from the group consisting of a foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.
When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .
When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, and especially in the case of spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.
In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.
When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.
When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.
The content of the active ingredient is not particularly limited, but may be included as 0.0001 to 10% by weight based on the total weight of the composition. When the active ingredient satisfies the content, it may exhibit excellent efficacy without side effects.
The cosmetic composition may further include functional additives and components included in the general cosmetic composition in addition to the prepared Hanhubak extract. The functional additives may include water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.
In addition to the said functional additive, you may mix | blend the component contained in a general cosmetic composition with the cosmetic composition of this invention as needed. In addition to the other components included, oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, blood circulation And accelerators, cooling agents, limiting agents, purified water, and the like.
Furthermore, the present invention relates to an external preparation for skin comprising the anti-aging composition, and the external preparation for skin is a generic term that can include anything applied outside of the skin, and cosmetics and medicines of various formulations can be included therein. The external preparation for skin is also not particularly limited, but may be, for example, an external preparation for preventing skin aging or improving skin wrinkles.
The present invention has found an excellent skin anti-aging effect using a composition containing Hanfubak extract which is a natural raw material. The anti-aging composition according to the present invention has a function of activating the retinoic acid receptor, specifically, retinoic acid receptor alpha or gamma, activation of luciferase, and inhibition of expression of collagenase, as can be seen in the test examples described below. It can prevent skin aging.
The present invention is described in more detail through the following implementation. However, the examples are provided to illustrate the present invention, and the scope of the present invention is not limited only to these examples.
Example 1 Preparation of Korean Herb Extract
Hanhubak was washed with purified water and dried, and then, 200 g of granulated Hanhubak powder was added to 1 liter of an aqueous 70% ethanol solution, boiled for 12 hours in an extractor equipped with a cooling condenser, filtered, and filtered through a 300 mesh filter cloth. After aging at 15 ° C. for 7 days, the filter was filtered using Whatman No. 2 filter paper. The extract was added to a 3-liter separatory funnel, and 1 liter of ethyl acetate was added thereto, shaken well to rot, and completely separated into two layers. The upper layer (ethyl acetate layer) was taken. The lower layer (water layer) was again extracted twice with a separatory funnel. The combined upper layers were combined, concentrated under reduced pressure at 50 ° C. using a distillation apparatus equipped with a cooling condenser, and dried to obtain a dry weight of 14.5 g.
Example 2 Preparation of Hanfu Park and Seomoktae Complex Extract
After washing Seomoktae with purified water and drying, 200g of granulated Seomoktae powder was put in 1 liter of 70% ethanol aqueous solution, boiled for 12 hours in an extractor equipped with a cooling condenser, filtered with 300 mesh filter cloth, and the filtrate was filtered at 4-15 ° C. After aging for 7 days, the filter was filtered using Whatman No. 2 filter paper. The extract was added to a 3-liter separatory funnel, and 1 liter of ethyl acetate was added thereto, shaken well to rot, and completely separated into two layers. The upper layer (ethyl acetate layer) was taken. The lower layer (water layer) was again extracted twice with a separatory funnel. The combined upper layers were combined, concentrated under reduced pressure at 50 ° C. using a distillation apparatus equipped with a cooling condenser, and dried to obtain a dry weight of 12.7 g.
Mass comparison of the extract of Hanhubak and Seomoktae extract obtained in Example 1 Mixed 1: 1.
Example 3 Preparation of Korean Herb Extract and Soritae Complex Extract
After washing frosted with purified water and drying, 200 g of crushed frosted powder was added to 1 liter of 70% ethanol aqueous solution, boiled for 12 hours in an extractor equipped with a cooling condenser and filtered with 300 mesh filter cloth, and the filtrate was filtered at 4-15 ° C. After aging for 7 days, the filter was filtered using Whatman No. 2 filter paper. The extract was added to a 3-liter separatory funnel, and 1 liter of ethyl acetate was added thereto, shaken well to rot, and completely separated into two layers. The upper layer (ethyl acetate layer) was taken. The lower layer (water layer) was again extracted twice with a separatory funnel. The combined upper layers were combined, concentrated under reduced pressure at 50 ° C. using a distillation apparatus equipped with a cooling condenser, and dried to obtain a dry weight of 16.8.
Mass ratio of the Hanhubak extract obtained from Example 1 and the frost extract Mixed 1: 1.
[Test Example 1] Wrinkle improvement effect
The collagenase expression inhibitory effect (producing ability) of each of the extracts obtained in Examples 1, 2 and 3 was measured as follows by using retinoic acid known to inhibit collagenase expression as a positive control group. First, human fibroblasts were placed in a 96-well microtiter plate containing Dulbecco's Modified Eagle's Media (DMEM) medium containing 2.5% fetal bovine serum to 5,000 cells / well. Incubated until 90% growth. After culturing in serum-free DMEM medium for 24 hours, and then treated Examples 1, 2 and 3 dissolved in serum-free DMEM medium with test substance for 24 hours at 100 μg / ml concentration and 1 μM of retinoic acid, Cell culture was collected. Then, the degree of collagenase production was measured using the collagenase measuring instrument (Amersham Pharma Co., Ltd.) from the collected cell culture solution. First, the cell culture medium collected in a 96-well plate uniformly coated with collagenase primary antibody was put in an antigen-antibody reaction for 3 hours. After 3 hours, the chromophore-bound secondary collagen antibody was placed in a 96-well plate and reacted for another 15 minutes. After 15 minutes, the coloring stimulant was added, causing color development at room temperature for 15 minutes, and 1M sulfuric acid was added again to stop the reaction (color development). The absorbance of the yellow 96-well plate was measured at 405 nm using an absorbance meter, and the expression level of collagenase was calculated by Equation 1 below, and the results are shown in Table 1 below. This is in contrast to the level of collagenase expression of the untreated group to 100. At this time, the cell culture liquid collected from the group not treated with the test substance was used as a control.
Equation 1
Collagenase expression level (%) = A / B × 100
A: absorbance of the test substance treatment cell group
B: absorbance of control group
As shown in Table 1, Hanhubak extract obtained from Example 1, Hanhubak Seomoktae complex extract obtained from Example 2 and Hanhubak Seoritae complex extract obtained from Example 3 effectively inhibited collagenase expression. I could confirm it.
[Test Example 2] Retinoic acid receptor alpha, gamma (RAR-α / γ) transcriptional activity effect
Retinoic acid receptor transcriptional activity was measured as follows for each of the extracts obtained in Examples 1, 2, and 3 using retinoic acid known to activate the retinoic acid receptor as a positive control group. First, the monkey kidney epithelial cell line CV-1 cells were inoculated into 24 well plates at a concentration of 4 × 104 cells / well, followed by incubation for 12-16 hours, before transfection. After washing with PBS (phosphate buffered saline) was changed the culture medium. Reporter plasmid, receptor plasmid, β-galactosidase expression vector and carrier DNA pBluscript (pBS) were combined to make a total of 0.5µg. After addition to each well of -1 cells, after treatment for 20 hours with 100 μg / ml concentration and 1 μM of retinoic acid in Examples 1, 2, and 3, 0.25 M Tris-HCl (pH 8.0) was added to the cell lysate. After preparing and transferring to a cell culture plate (Costar) for 96-well Luminometer, luciferase activity was measured through Luciferase Assay SystemTM kit (Promega Corporation) as recommended by the supplier. Beta-galactosidase activity was measured by transferring 20 µl of the cell lysate to 96-well assay plate (Falcon, Cat. No. 353911), and then 100 µl of ONPG (Onitrophenyl β-) in each well. galacti-pyranoside) solution was added and left for 2 hours in a 37 ° C. incubator. Thereafter, 50 μl of 1 M sodium carbonate (Na 2 CO 3 ) solution was added to each well, and the absorbance was measured at 415 nm. The activity of beta galactosidase measured by the above method was used to correct the efficiency of transformation in the cell lysate, and to measure the relative activity of luciferase. .
Relative activity (%)
Relative activity (%)
As shown in Table 2, the extract of Hanhubak extract obtained from Example 1, Hanhubak Seomoktae complex extract obtained from Example 2 and Hanhubak Seoritae complex extract obtained from Example 3 were excellent in retinoic acid receptor alpha, gamma (RAR -α / γ) it was confirmed that the effect of the transcriptional activity.
Although the formulation example of the composition containing Hanfubak extract of this invention is demonstrated below, the composition of this invention is not limited only to this example.
[ Formulation Example 1] lotion formulation
Herbal Extract of Example 1 3.00
L-ascorbic acid-2-magnesium phosphate salt 1.00
Water Soluble Collagen (1% Aqueous Solution) 1.00
Sodium Citrate 0.10
Citric Acid 0.05
Licorice Extract 0.20
1,3-butylene glycol 3.00
Purified water balance
(Unit: wt%)
[ Formulation Example 2] Cream type Formulation
Hanfubak Seomoktae composite extract of Example 2 1.00
Polyethylene Glycol Monostearate 2.00
Self-emulsifying glycerin monostearate 5.00
Cetyl Alcohol 4.00
Squalene 6.00
Tri-2-ethylhexane glyceryl 6.00
Sphingolipids 1.00
1,3-butylene glycol 7.00
Purified water balance
(Unit: wt%)
[ Formulation Example 3] Packed Formulation
Hanfubak Soritae complex extract of Example 3 5.00
Polyvinyl Alcohol 13.00
L-ascorbic acid-2-magnesium phosphate salt 1.00
Lauroylhydroxyproline 1.00
Water Soluble Collagen (1% Aqueous Solution) 2.00
1,3-butylene glycol 3.00
Ethanol 5.00
Purified water balance
(Unit: wt%)
[ Formulation Example 4] Cosmetic liquid type Formulation
Korean Herbal Extract 2.00
Hydroxyethylene Cellulose (2% Aqueous Solution) 12.00
Xanthan gum (2% aqueous solution) 2.00
1,3-butylene glycol 6.00
Dark Glycerin 4.00
Sodium Hyaluronate (1% aqueous solution) 5.00
Purified water balance
(Unit: wt%)
Claims (10)
The composition is an anti-aging composition that further contains any one or more of Seomoktae extract and Seoritae extract as an active ingredient.
The extract is an anti-aging extract of any one or more solvents selected from the group consisting of anhydrous and hydrous lower alcohols having 1 to 4 carbon atoms, acetone, butylene glycol, ethyl acetate, diethyl acetate, diethyl ether, benzene, chloroform and hexane Composition.
The anti-aging composition of the Hanhubak extract is 0.0001 to 10% by weight based on the total weight of the composition.
The content of the Seomoktae extract is 0.0001 to 10% by weight based on the total weight of the composition.
The content of the frost extract is an anti-aging composition of 0.0001 to 10% by weight based on the total weight of the composition.
The composition is an anti-aging composition to prevent skin aging through the activation of the retinoic acid receptor.
The retinoic acid receptor is a retinoic acid receptor alpha or gamma composition for anti-aging.
The retinoic acid receptor is luciferase (Luciferase) is an anti-aging composition.
The composition is an anti-aging composition to prevent skin aging through inhibiting the expression of collagenase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020110102026A KR20130037563A (en) | 2011-10-06 | 2011-10-06 | Compositions containing an extract of machilus thunbergii siebold zuccarini for anti-aging through activation of retinoic acid receptor |
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| KR1020110102026A KR20130037563A (en) | 2011-10-06 | 2011-10-06 | Compositions containing an extract of machilus thunbergii siebold zuccarini for anti-aging through activation of retinoic acid receptor |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020040621A1 (en) * | 2018-08-23 | 2020-02-27 | 주식회사 엘지생활건강 | Composition for promoting differentiation or proliferation of human adipose stem cells |
| CN112638357A (en) * | 2018-08-23 | 2021-04-09 | 株式会社Lg生活健康 | Composition for promoting differentiation or proliferation of human adipose-derived stem cells |
-
2011
- 2011-10-06 KR KR1020110102026A patent/KR20130037563A/en active Search and Examination
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020040621A1 (en) * | 2018-08-23 | 2020-02-27 | 주식회사 엘지생활건강 | Composition for promoting differentiation or proliferation of human adipose stem cells |
| CN112638357A (en) * | 2018-08-23 | 2021-04-09 | 株式会社Lg生活健康 | Composition for promoting differentiation or proliferation of human adipose-derived stem cells |
| CN112638357B (en) * | 2018-08-23 | 2024-03-08 | 株式会社Lg生活健康 | Composition for promoting differentiation or proliferation of human fat-derived stem cells |
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