KR20130020390A - Solubilization of anglica gigas nakai by extrusion processing - Google Patents

Abstract

PURPOSE: A solubilization method of Anglica gigas Nakai through an extrusion molding process is provided to enhance the yield and solubility of an Anglica gigas Nakai extract. CONSTITUTION: Anglica gigas Nakai is solubilized using a biaxial extruder. The biaxial extruder comprises an ingredient supply unit, an ingredient kneading disk, an extrusion die, a forward transfer screw, a reverse transfer screw, and a barrel heater. The solubilization of Anglica gigas Nakai improves the yield and water solubility of an Anglica gigas Nakai extract. A composition for antioxidation contains the Anglica gigas Nakai extract.

Description

Solubilization of Anglica gigas Nakai by Extrusion processing

The present invention relates to a method for accommodating chaff of Angelica gigas using an extrusion molding process, and more particularly, an optimum extrusion molding process for increasing the yield of Champignon Angelica extract, increasing the water solubility of the extract, and increasing the yield of indicator and useful components. The present invention relates to a method for accepting a true donkey that derives conditions.

Angelica is the most widely used natural product among herbal medicines. As in the Angelica Angelica (Angelica) plant belonging to the Apiaceae, Angelica gigas Korea (Angelica gigas Nakai), Chinese donkey ( Angelica) sinensis Diels), In Japan, the ildanggwi (Angelica acutiloba Kitagawa) in origin. The roots of the Angelica are collected before the flowering of the Angelica Angelica, and the root portion of the Angelica is known to have a blood-replenishing effect to replenish the blood. Therefore, it is known to be prescribed as a blood-reducing agent for anemia and gynecological diseases. Roots of Angelica gigas are coumarine derivatives such as decusin, decosinol and nodakenin, and α-pinene, limonene and β-eudesmol. And an essential oil component containing elemol and the like as a main component. The main component that shows the pharmacological action of Angelica gigas is decursin, a cumarin derivative, and active ingredients such as decursinol, umbelliferon and beta-sitosterol. It is contained. Cumin derivatives are known to have been used during acute myocardial infarction as anticoagulants used to prevent hemorrhage in the past. In particular, new and Decker when isomers Decker play Ansel rate (decursinol angelate) is ildanggwi and Chinese angelica root, it does not exist, only Angelica gigas native (A ngelica Korea gigas Nakai) is only present in the rhizome (rhizome).

These Angelica gigas have recently been reported to have antimutagenic activity, cancer cell growth inhibitory activity, liver detoxification activity and the like. These donkeys are recently developed as tablets, pouches, and the like as raw materials for functional food materials, and many studies have been made on the new use of donkeys.

However, the immunomodulators of Angelica gigas obtained by the conventional methods of extracting synthetic and other natural products have the biggest problem of low production efficiency compared to the activity of extracts.

In addition, the extraction method using ultrasonic waves, which is a technology that utilizes enhancement of penetration force and vibration effect through ultra-fine vibration, increases the frequency of waves propagated while being compressed and expands, which is conventionally formed to elasticity to improve extraction yield. It was not efficient because of the time and cost compared to the activity and immune activity.

Therefore, the problem to be solved by the present invention has an effect of increasing the yield compared to the conventional extraction method, the development of such extract as a raw material in the Angelica gigas extract extrusion molding method, which can bring the production cost reduction effect, true It is to provide a method for accepting Angelica gigas using an optimal extrusion process for increasing the yield of Angelica extract, increasing the water solubility of the extract, and increasing the yield of indicator and useful components.

In order to achieve the above object,

In the true donkey receptacle method using the coaxial full-engagement twin-screw extrusion machine,

The twin screw extrusion machine includes a raw material supply unit; Raw dough disc; Extrusion die; Forward feed screw; Reverse feed screw; And a barrel heating unit;

The barrel temperature is 140-160 ℃, the screw speed is 90-100 rpm, raw material input amount of 28-32 kg / h, water input amount of 4.0-4.5 kg / h, characterized in that the Angelica Angelica

The barrel temperature is 140-160 ℃, the screw speed is 65-75 rpm, the raw material input amount 40-50 kg / h, the moisture input amount 4.0-7.0 kg / h, characterized in that

The barrel temperature is 140-160 ℃, the screw speed of 85-90 rpm, the raw material input amount of 30-40 kg / h, the water input amount provides 4.0-5.0 kg / h provides a donkey receptacle receiving method. .

The present method for accommodating chaff of the Angelica gigas using the extrusion process according to the present invention shows the optimum process conditions for extrusion molding for increasing the yield of the Champs perilla and the useful components, and increasing the solubility of the polysaccharides of Champs perilla. It is possible to obtain and isolate the Angelica extract, and it is possible to develop an antioxidant active composition, a functional food, and a functional cosmetic including the same.

1 is a schematic view schematically showing an extrusion molding apparatus used in the present invention.
Figure 2 is an image showing the appearance of the Angelica Extruded Moldings according to the extrusion process conditions (Example 3) according to an embodiment of the present invention.
Figure 3 is an image showing the turbidity of the extract of Angelica gigas extract according to an embodiment of the present invention.
4 is a contour plot analysis of the extrusion molding process for increasing the extraction yield of Angelica gigas in the selection of the optimum conditions of the extrusion molding process.
FIG. 5 is a contour plot analysis of an extrusion molding process for increasing water solubility in Angelica gigas in selecting an optimal condition of an extrusion molding process.
FIG. 6 is a contour plot analysis of an extrusion process variable for increasing the extraction of the Angelica gigas in selecting the optimum conditions of the extrusion process.
7 is a graph showing the results of DPPH radical scavenging activity analysis of the Angelica gigas Extruded in the antioxidant activity evaluation of the Angelica gigas Root Extrusion.
8 is a graph showing the results of analysis of the ABTS + radical scavenging ability of the Angelica gigas Extruded in the antioxidant activity evaluation of the Angelica gigas Root Extrusion.

Hereinafter, the present invention will be described in more detail.

The present invention provides a method for receptivating a true Angelica gigas extract with optimum process conditions for increasing the yield of the Angelica gigas extract, the water solubility of the extract of Angelica gigas, and the yield of the indicator and useful components of the Korean Angelica gigas.

For the experimental design of the reaction process of the extrusion process, the preliminary experiments on the screw rotation speed, the raw material feeding rate, and the water feeding rate conditions were first performed. Through the preliminary experiments, the screw rotational speed was 40-80 rpm and the raw material feeding rate was 30. The extrudates of Angelica gigas were prepared at -40 kg / h and 3-6 kg / h water input rates, and optimal process conditions were selected through characterization.

In the method of retracting Angelica gigas using a coaxial full-engagement twin screw extrusion machine, the twin screw extrusion machine includes a raw material supply part, a raw dough disc, an extrusion die, a forward feed screw, a reverse feed screw, and a barrel heating part, and extracts the true donkey The barrel temperature is 140-160 ° C., the screw speed is 90-100 rpm, the raw material input is 28-32 kg / h, and the water input is 4.0-4.5 kg / h. It provides a method for accepting Angelica gigas.

In addition, as an optimum condition for improving the water solubility of the Angelica Angelica extract, the barrel temperature is 140-160 ℃, the screw speed 65-75 rpm, the raw material input amount 40-50 kg / h, the water input amount 4.0-7.0 It provides a Angelica Angelica receptive method characterized in that the kg / h.

In addition, as an optimum condition for improving the yield of Decusin and Decusinol Angelate contained in Angelica gigas extract, the barrel temperature is 140-160 ℃, the screw speed 85-90 rpm, raw material input amount 30-40 kg / h, the water input amount 4.0-5.0 kg / h provides a Angelica Angelica solubilization method.

Extraction yield and water solubility of the Angelica Angelica extruded moldings were confirmed to have an effect of increasing up to 21.75%, 15.26% as compared to the control. In addition, it was confirmed that the polyphenol and flavonoid useful component contents were increased through the physicochemical analysis of the Angelica gigas Extruded Molding, and that the contents of dequesin and decosinol angelate, which are representative indicators of the Angelica gigas, increased by 17.42%. Confirmed. Therefore, the present invention will bring the effect of reducing the cost of extract raw material production cost for food and cosmetic material development.

Hereinafter, the present invention will be described in more detail with reference to preferred embodiments. It will be apparent to those skilled in the art, however, that these examples are provided to further illustrate the present invention, and the scope of the present invention is not limited thereto.

<Examples>

Example 1 Pretreatment of Angelica Angelica Raw Materials Before Extrusion Molding Process

Three hundred kg of Angelica gigas roots, which were produced according to Good Agricultural Practices (GAP) in Jinbu, Gangwon-do, were washed three times. The washed roots were cut by stem roots and washed three times again to completely remove soil, and then cut by 0.3-0.5 cm thick. The Angelica gigas root section was rapidly frozen at -80 ° C and then dried in a mass freeze dryer for 72 hours to recover 64 kg of sections with a moisture content of 1.5% or less. The dried slices were coarsely ground using a hammer mill and finely ground to 60 mesh or less with a pin mill to prepare 60 kg of Angelica gigas root fines.

Example 2 Preliminary Experiment for Optimal Process Design of Angelica Angelica Extrusion

As the extrusion apparatus used in the present invention, a coaxially fully engaged biaxial extrusion machine (Corotating, intermeshing Brothers Co., Switzerland) having a ratio of length and diameter of barrel (L / D) of 20 was used. The conditions of the extrusion molding apparatus used in the present invention are shown in the following [Table 1], and a schematic diagram of the apparatus is shown in FIG. Detailed conditions are as follows, and the conditions for each preliminary experiment are shown in the following [Table 2].

The screws are combined so that the pitch size of the forward feed screw is reduced in the order of 66 mm, 44 mm, 33 mm from the feed of the raw material, and the pitch size is 14.67 mm in the heating parts (barrels 2 and 3) to induce strong laminar rolling. Two reverse screw elements (RSM) and one kneading disk element (KD) were kneaded, and the extrusion die was equipped with a 3 mm round type. For the experimental design of the extrusion treatment, the barrel temperature and the raw material input were fixed at 150 and 17 kg / h, the screw rotation speed was 40-60 rpm, and the water input was extruded at 1.5-4 kg / h. Extruded Angelica roots were hot-air dried at 50 ° C. for 24 hours, and then pulverized with a pin mill of 60 mesh or less and used as an analytical sample.

Extrusion Machine Co-Operating Fully Interlocking Extruder
(Buhler Brothers Co., DNDL-44, Uzwil, Switzerland) Raw material max
Feed rate 140 Kg / h die Round Type 3mm Barrel
Length / Diameter 20 2,3 barrel heating Barrel temperature 150 Screw combination 66 ¹⁾ R ^{2 )} * 3 ³⁾ KD ^{4 )} (RL ^{5 )} R) * 3 44R * 5 RSE ^{6 )} (LR) 44R * 4 RSE (LRL) 33R * 5 ST ^{7 )}

1) screw pitch, 2) right handed, 3) the number of screw, 4) KD: kneading disk element, 5) L: left handed, 6) RSE: reverse screw element, 7) ST: screw top

division Torque value
(%) screw
Rotation speed
(rpm) Raw material
input
(kg / h) moisture
input
(kg / h) Raw material + moisture
input
(kg / h) Raw material
Moisture content
(%) dough
Moisture content
(%) Barrel
Temperature
(℃) Mechanical
energy
Consumption rate
(KJ / kg) One 41 60 17 4.0 21.0 1.07 19.9 132 440.4 2 37 60 17 3.0 20.0 1.07 15.9 137 417.3 3 38 60 17 2.0 19.0 1.07 11.5 145 451.2 4 42 40 17 5.2 22.2 1.07 24.2 140 284.5 5 53 40 17 1.5 18.5 1.07 9.1 151 430.9

Example 3 Optimal Process Design of Angelica Extrusion

The extrusion molding machine for selecting the optimum process for the Angelica gigas extrusion molding was used in the same coaxial full-engagement twin-screw extruder as in the apparatus used in Example 2, and the screw was combined under the same conditions.

Extrusion process variables affecting decursin and decurcinol angelate, which are indicator components of Angelica gigas Extruded Molds: Screw Rotation Speed (X1), Raw Material Input (X2), Water Input (X3) We determined three independent variables. The barrel temperature of the extrusion process is fixed at 150 ° C, the screw rotation speed is 40-80 rpm, the raw material input is 30-40 kg / h, and the water input is 3-6 kg / h. Prepared.

The maximum, minimum and median values of the independent variables for the optimization of process conditions were selected as the results of preliminary experiments. Indicated. The optimal process conditions were selected by statistical analysis of response surface methodology. Table 3 shows the extrusion conditions, and Table 4 shows the conditions for extruding Angelica gigas.

division Encoding Independent variable X1 X2 X3 screw
Rotation speed
(rpm) Raw material
input
(kg / h) moisture
input
(kg / h) One -One +1 0 40 40 4.5 2 -One 0 +1 40 35 6.0 3 0 -One +1 60 30 6.0 4 -One +1 -One 40 40 3.0 5 0 +1 +1 60 40 6.0 6 +1 -One 0 80 30 4.5 7 -One -One 0 40 30 4.5 8 +1 0 +1 80 35 6.0 9 0 0 0 60 65 4.5 10 -One 0 0 40 35 4.5 11 +1 +1 0 80 40 4.5 12 +1 -One +1 80 30 6.0 13 +1 0 -One 80 35 3.0 14 0 +1 -One 60 40 3.0 15 +1 +1 +1 80 40 6.0

division Torque value
(%) screw
Rotation speed
(rpm) Raw material
input
(kg / h) moisture
input
(kg / h) pressure
(bar) Raw material
Moisture content
(%) dough
Moisture content
(%) Barrel
Temperature
(℃) Mechanical
energy
Consumption rate
(KJ / kg) One 58.5 40 40 4.5 17.5 1.1 11.1 139.0 197.7 2 44.5 40 35 6.0 17.0 1.1 15.6 138.5 163.2 3 32.5 60 30 6.0 17.0 1.1 17.6 139.5 203.7 4 68.5 40 40 3.0 18.0 1.1 8.0 143.0 239.6 5 40.5 60 40 6.0 18.0 1.1 14.0 146.0 198.6 6 31.0 80 30 4.5 18.0 1.1 14.0 146.0 270.3 7 38.5 40 30 4.5 17.0 1.1 14.0 140.0 167.8 8 34.5 80 35 6.0 18.0 1.1 15.6 145.0 253.1 9 39.0 60 35 4.5 18.0 1.1 12.4 145.0 222.7 10 46.0 40 35 4.5 17.5 1.1 12.4 141.5 175.1 11 37.5 80 40 4.5 18.5 1.1 11.1 151.0 253.5 12 30.5 80 30 6.0 18.0 1.1 17.6 146.5 254.8 13 34.5 80 35 3.0 18.5 1.1 8.9 152.5 273.1 14 50.5 60 40 3.0 18.5 1.1 8.0 156.5 264.9 15 37.5 80 40 6.0 18.0 1.1 14 146.5 245.2

Experimental Example 1. Analysis of common components of Angelica gigas

According to the AOAC method (1990), the general component analysis of Angelica gigas was quantified by 105 ° C atmospheric pressure drying, crude fat as Soxhlet extraction, crude protein as semi-micro-Kjedahl method, and crude ash as direct ashing method.

Experimental Example 2. Evaluation of Physicochemical Properties and Useful Components of Angelica Angelica Extrusion

(1) Evaluation of Physicochemical Properties of Extracts

end. Yield Analysis

200 mL of water was added to 10 g of Angelica gigas root extrudates, followed by high pressure extraction at 121 ° C. for 20 minutes to analyze the yield.

I. Water Solubility Index (WSI) Analysis

Water solubility index (WSI) was measured by modifying Anderson's method. Centrifuged for 20 minutes at 3,000 × g, extracted from Angelica Root hot water extract for yield analysis, filtered using filter paper (No 4, Whatman), 5 mL of the supernatant was added to a water weighing bottle and After drying for a time to measure the solid content, WSI was determined by the following equation.

Water Solubility Index (%) = {[(Soluble Solid Weight (g) / 5 mL) × 100 mL] / 10 g} × 100

All. Water Adsorption Index (WAI) Analysis)

Water absorption index (WAI) is the basis weight of the precipitate centrifuged in the above experiment, and the weight of the precipitate is dried in a dryer (50 ℃) and the basis weight is expressed as the amount of water absorbed per gram of dry sample (mL / g) It was.

Water adsorption index = weight of sediment (g) / weight of dry solids (g)

(2) Evaluation of useful ingredient content of extract

end. Total Sugar Content Analysis

The total sugar contained in the extract was measured by modifying the phenol-sulphuric reaction method. 0.5 ml of the sample was taken in a test tube, 0.5 ml of 5% phenol solution was added, and 2.5 ml of sulfuric acid was added and mixed quickly. After reacting at room temperature for 20 minutes, the result was obtained by measuring absorbance at 490 nm. The result was multiplied by 0.9 and converted into sugar content. Glucose was used as a standard.

I. Total Polyphenol Content Analysis

The determination of total polyphenols was made by modifying the Folin-Denis method. 0.2 mL of the sample was taken in a test tube, distilled water was added to make 1 mL, and 0.1 mL of Folin-ciocalteu's phenol reagent was added thereto, mixed, and left to stand at room temperature for 3 minutes. 0.2 mL of saturated Na 2 CO 3 solution was added, mixed, distilled water was added to make 2 mL, and the mixture was left at room temperature for 1 hour, and centrifuged at 3,000 × g and 20 ° C. for 10 minutes. After taking the supernatant and measuring the absorbance at 725 nm, the total polyphenol content was determined from the standard curve prepared using catechin.

The control was treated in the same manner as above using 50% MeOH solution instead of the sample.

All. Total Flavonoid Content Analysis

The total flavonoid content was measured by adding 4 mL of 90% diethylene glycol to 400 μl of ethanol extract sample, 40 μl of 1 N NaOH, and incubating for 1 hour in a 37 ° C. water bath after spectrophotometer Absorbance was measured at 420 nm. Naringin was used as a standard reagent.

la. Content Analysis of Korean Angelica Angelica (Decusin and Decusinol Angelate) Contents

Decursin and decursinol angelate, the index components of Angelica gigas, were analyzed using HPLC (HP 1100, Agilent Technologies, U.S.A). After extracting 1 g of Angelica extrudates, 50 mL of 80% MeOH was added, and the residue was re-extracted by adding 50 mL of 80% MeOH and filtered through a 0.45 μm membrane filer to be used for analysis. The Zorbax SB-C18 column was used for the HPLC analysis of the indicator, and the solvent phase was analyzed by gradient acetonitrile from 60% to 100% for 29 minutes using acetonitrile and water. The solvent phase rate was 0.8 mL / min, oven temperature 35 ° C., the sample injection amount was 20 μl, and the analysis wavelength was performed under the conditions of 280 nm. [Table 5] below shows HPLC analysis conditions for glycoproteins.

HPLC system G1379A Degasser Pump G1311A Quat-pump Detector G1314A VWD Column Zorbax SB-C18 Mobile phase A: Acetonitrile, B: H ₂ O Gradient Time (min) 0-20 20-25 25-29 29-30 30-35 % A 60-85 85-100 100 100-60 60 Flow rate 0.8 mL / min Injection 20 μl Column Temp. 35 ℃ Detection 280 nm

Experimental Example 3. Evaluation of Antioxidant Activity of Angelica gigas Root Extrusions

(1) DPPH radical scavenging ability

The reducing power of each sample to DPPH (2,2-diphenyl-1-picrylhydrazyl) radical was measured. 100 uM DPPH solution was prepared by dissolving 3.943 mg DPPH in 80% MeOH solvent. 0.1 mL of the sample and 2.9 mL of 100 uM DPPH solution were mixed and reacted for 30 minutes by blocking light at 23 ° C., and then absorbance (Jasco V550) was measured at 517 nm.

For the activity comparison, ascorbic acid, β-carotene and dl-α-tocopherol were used. As a control, 0.1 mL of 80% methanol was added instead of the sample and 2.9 mL of 80% methanol was added instead of the DPPH solution. Measured. The free radical scavenging activity of each sample was expressed as an IC50 value, which is the concentration of the sample required to reduce the inhibitory activity (%) for each sample concentration to 50% for the control.

(2) ABTS + radical scavenging ability

Trorolox Equivalent Antioxidant Capacity (TEAC) was analyzed by modifying Roberta et al. As a method of electron transfer antioxidant capacity.

ABTS (2,2'-azonobis (3-ethylbenzothiazolin-6-sulphonate)) reacts with free radicals (hydroxy, peroxyl, alkoxyl, ignorganic radical) to produce stable cations (ABTS +), thus reacting ABTS solution with potassium persulfate solution It was measured by the reduction reaction of the generated ABTS + radicals.

12-16 hours before the experiment, 7 mM ABTS + solution was added 2.45 M potassium persulfate and stored in a brown reagent bottle. Immediately before analysis, 100 μM ABTS + solution was prepared by diluting the ABTS + solution to 1/70. 10 μl of the sample dissolved in 70% MeOH and 990 μl of a 100 uM ABTS + solution were mixed and allowed to stand at room temperature dark for 20 minutes, and then absorbance was measured at 734 nm. For comparison of activity, Trolox (6-hydroxy-2.5.7.8-tetramethychroman-2 carboxylic acid) and Ascorbic acid were used. For the control, 10 μl of DMSO was added instead of the sample, and 990 μl of 70% MeOH was used instead of the ABTS + solution. The addition was measured. Inhibitory activity (%) for each sample concentration for the control was calculated as follows, and as a result IC50 value.

Inhibition == (Control Absorbance-Sample Absorbance-Sample Blank Absorbance) / Control Absorbance] x 100

Evaluation Example 1. Angelica Angelica Fine Powder

According to Experimental Example 1, the results of analyzing the general components of the true Angelica pulverulent powder are shown in the following [Table 6]. As a result of analysis, the moisture content was 1.07%, the ash content was 5.73%, and the protein was contained 1.38%. Confirmed. The fat content is 8.60%, which contains a large amount compared to ash or protein, confirming the possibility of developing essence oils and fragrances using the unique fragrances of Angelica.

division moisture(%) Ashes (%) Fat(%) protein(%) Angelica Angelica Derivative 1.07 ± 0.05 5.73 ± 0.02 8.60 ± 032 1.38 ± 0.01

Evaluation Example 2 Appearance Characteristics of Angelica Angelica Extruded Moldings

As the screw speed and the input amount of the raw material were set at the same condition at 60 rpm and 40 kg / h, and the extrusion process was carried out with different water input, the sample No. 5 with a water input of 6 kg / h was highly swelled by hydrolysis. In comparison, 14 samples with 3.0 kg / h of water input showed pellet form in which tissue was easily broken.

In addition, sample 1, which was extruded at a screw speed of 40 rpm, a raw material input amount of 40 kg / h, and a water input amount of 4.5 kg / h, was prepared as an extrudate in the form of a very hard pellet. Therefore, the moisture input among the process variables of the extrusion molding was found to have a significant effect on the appearance change of the tissue.

Figure 2 shows the appearance of the true donkey extrusion molding according to the extrusion process conditions 1, 5, 14 in order from the left side.

Evaluation Example 3 Physicochemical Properties of Angelica Angelica Extrusion Molding According to Example 2

The physicochemical properties of the Angelica Angelica Extruded Molds according to the preliminary experiment of Example 2 are shown in the following [Table 7].

Yields of extruded C. chinensis extracts were increased by 25% compared to those of the non-extruded hot water extract control, and water solubility increased by 20% (p <0.001). These results were consistent with the results of the extruded samples showing a higher turbidity in comparison to the control in the external characteristics of the hot water extract (FIG. 3). On the contrary, the water absorbency and water retention were decreased compared to the control by decomposing the polymer polysaccharides by the action of high temperature, high pressure and shearing force during the extrusion molding process. The contents of decansin and decusinol angelate, which are indicators of Angelica gigas, increased by 7-9% (p <0.05) in the extruded samples compared to the non-extruded controls.

division yield
(%) moisture
Solubility
(%) moisture
Adsorption degree
(mL / g) moisture
Holding power
(mL / g) Decker
(%) Dekersinol
Angelate
(%) Control 40.61 ± 0.15 53.27 ± 0.20 8.84 ± 0.43 1.64 ± 0.00 3.46 ± 0.06 2.23 ± 0.05 One 52.71 ± 0.36 62.42 ± 0.43 6.25 ± 0.02 0.56 ± 0.01 3.78 ± 0.11 2.44 ± 0.07 2 51.10 ± 0.43 62.15 ± 0.52 6.37 ± 0.10 0.67 ± 0.00 3.65 ± 0.03 2.36 ± 0.02 3 50.78 ± 0.12 60.94 ± 0.15 6.25 ± 0.08 0.63 ± 0.01 3.71 ± 0.04 2.38 ± 0.03 4 52.27 ± 0.27 62.73 ± 0.33 6.37 ± 0.04 0.64 ± 0.02 3.75 ± 0.03 2.41 ± 0.02 5 52.25 ± 0.36 62.21 ± 0.43 6.43 ± 0.06 0.68 ± 0.01 3.68 ± 0.13 2.37 ± 0.08

Evaluation Example 4. Physicochemical Properties of Angelica Angelica Extruded Moldings According to Example 3

The physicochemical properties of the Angelica Angelica extrudate according to Example 3 for the selection of an optimal process for extrusion molding are shown in the following [Table 8]. The yield, water solubility, water absorption, total sugar, total polyphenol, and total flavonoid content of Table 8 are the analysis results of the hot water extract samples, and the dekerin and dekersinol angelates are the analysis results of the 80% methanol extract samples. .

The yield and water solubility of the extruded samples were increased by 9.84-27.79% and 3.04-18.00%, respectively, and the total sugar was increased by 10.99-37.27%, compared to the non-extruded control 1. In general, total polyphenol and total flavonoid extraction contents showing antioxidant activity were significantly increased (p <0.05) to 22.22-74.60% and 45.65-73.91%, respectively.

In contrast, the water absorption of the extruded sample decreased compared to the control. This was presumed to be due to decomposition of polymer polysaccharides such as pectin, cellulose and hemicellulose by high temperature, high pressure and shear force during extrusion.

In addition, the content of dekersin and dekersinol angelate, which are indicator components of Angelica gigas, was 4.14-21.09% as a result of HPLC analysis, and it was confirmed that the extraction rate of the index component was greatly increased.

division yield
(%) moisture
Solubility
(%) moisture
Absorbance
(mL / g) Total
(%) Total Polyphenols
(%) Total Flavonoids
(%) Decker
+
Dekersinol
Angelate
(%) Control 1 44.40 63.43 10.42 29.65 0.63 0.46 5.31 One 48.77 68.20 8.39 36.37 0.93 0.72 5.79 2 54.23 74.85 8.00 38.83 0.95 0.78 5.98 3 54.07 69.33 8.22 38.54 0.95 0.78 6.37 4 56.74 72.28 9.00 36.70 0.77 0.67 5.57 5 53.99 67.49 7.82 40.70 0.98 0.80 6.25 6 54.39 67.56 7.74 32.91 0.85 0.67 6.43 7 53.40 67.17 7.80 40.66 0.97 0.77 6.32 8 54.40 69.29 8.12 39.18 1.02 0.79 6.33 9 52.41 65.51 8.43 37.72 0.97 0.77 5.92 10 52.43 66.37 8.83 38.93 1.02 0.79 6.02 11 53.97 67.47 7.96 36.08 0.90 0.68 5.70 12 53.50 67.72 8.08 35.90 1.00 0.68 6.31 13 52.56 65.70 7.81 37.20 1.03 0.72 5.53 14 52.94 65.36 7.51 37.25 1.10 0.73 5.69 15 53.95 67.02 7.97 38.48 1.01 0.79 6.00 Control 2 46.44 62.89 5.75 25.46 0.53 0.39 -

Control 1: Unextruded Angelica powder (121 ° C., 20 minutes)

1-15: Extrusion Molded Sample Angelica Powder (121 ° C., 20 minutes)

Control 2: Chinese Herbal Angelica Angelica (using a general medicine bath, 100 ℃, 2 hours)

Evaluation Example 5. Selection of Optimum Condition for Extrusion Process

Contour plot analysis was performed using SAS statistical program to select the optimal condition of the extrusion treatment process based on the physicochemical characterization of the extruded molding. In Figure 4, the extrusion process variable contour contour analysis for increasing the water solubility of Angelica Angelica is shown in Figure 5, the extrusion process parameter contour contour analysis for increasing the extraction of the true Angelica Angelica are shown in Figure 6, respectively.

In order to increase the yield of hot water extract, the optimum screw speed was 94.11 rpm, raw material input was 30.53 kg / h, and water input was 4.12 kg / h at 150 ℃ barrel temperature. In addition, the screw speed of 70.56 rpm, raw material input 45.98 kg / h, water input 5.29 kg / h were selected as the optimum conditions to increase water solubility. The optimum conditions for increasing the extraction yield were analyzed as the screw speed 88.48 rpm, raw material input 33.97 kg / h, and water input 4.51 kg / h.

Evaluation Example 6 Antioxidant Activity of Angelica gigas Root Extrusions

DPPH radical scavenging activity and ABTS + radical scavenging activity were evaluated only in samples 3, 5, 6, 7, 8, and 12 having high yield and extraction rate of useful components in the extruded samples.

DPPH radical scavenging ability was 139.89 (± 0.61) μg / mL for sample 7 and 154.93 (± 0.09) μg / mL for sample 1, whereas the IC50 value of unextruded control 1 was 163.78 (± 0.35) g / mL. High in mL. ABTS + radical scavenging ability was the highest at 110.07 (± 1.29) μg / mL in sample 3, whereas the IC50 value of non-extruded control 1 was 122.69 (± 0.66) g / mL. Except for 5, 6, 7, 12 samples were all high.

As a result, it was confirmed that the antioxidant activity by extrusion molding increased significantly (p <0.05), although it did not meet the general antioxidant ascorbic acid.

DPPH radical scavenging activity analysis results of the Angelica Angelica Extrusions are shown in FIG. 7 and ABTS + radical scavenging activity analysis results of the Angelica Angelica Extrusions are shown in FIG. 8.

Claims (7)

In the true donkey receptacle method using the coaxial full-engagement twin-screw extrusion machine,
The twin screw extrusion machine includes a raw material supply unit; Raw dough disc; Extrusion die; Forward feed screw; Reverse feed screw; And a barrel heating unit;
The barrel temperature is 140-160 ℃, the screw speed is 90-100 rpm, raw material input 28-32 kg / h, water input amount 4.0-4.5 kg / h characterized in that the Angelica Angelica.
The method of claim 1,
The Angelica Angelica solubilization method is characterized in that to improve the yield of Angelica Angelica Angelica.
In the true donkey receptacle method using the coaxial full-engagement twin-screw extrusion machine,
The twin screw extrusion machine includes a raw material supply unit; Raw dough disc; Extrusion die; Forward feed screw; Reverse feed screw; And a barrel heating unit;
The barrel temperature is 140-160 ℃, the screw speed is 65-75 rpm, raw material input amount 40-50 kg / h, water input amount 4.0-7.0 kg / h characterized in that the Angelica Angelica.
The method of claim 3, wherein
The Angelica Angelica solubilization method is characterized in that to improve the water solubility of Angelica Angelica extract.
In the true donkey receptacle method using the coaxial full-engagement twin-screw extrusion machine,
The twin screw extrusion machine includes a raw material supply unit; Raw dough disc; Extrusion die; Forward feed screw; Reverse feed screw; And a barrel heating unit;
The barrel temperature is 140-160 ℃, the screw speed 85-90 rpm, raw material input amount 30-40 kg / h, water input amount 4.0-5.0 kg / h characterized in that the Angelica Angelica.
The method of claim 5, wherein
The Angelica Angelica solubilization method is characterized in that to improve the yield of Decacin and Decacinol Angelate.
The composition for antioxidant activity comprising the extract of Angelica gigas extract extrusion treatment according to any one of claims 1 to 6.

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