KR20120122664A - Composition for preventing and treating obesity comprising Angelica Keiskei Koid. extract - Google Patents

Abstract

PURPOSE: A composition containing Angelica Keiskei Koid. extract for preventing and treating obesity is provided to reduce body weight and body fat. CONSTITUTION: A food composition for preventing and treating obesity contains Angelica Keiskei Koid. extract as an active ingredient. The extract is a non-soluble fraction of Angelica Keiskei Koid. organic solvent extract. A method for preparing the extract comprises: a step of preparing organic solvent extract of Angelica Keiskei Koid.; a step of adding water to the extract; and a step of removing water soluble ingredients. The organic solvent is alcohol of 1-6 carbon atoms, acetone, ethyl acetate, n-hexane, diethyl ether, acetone, or benzene. A pharmaceutical composition for preventing and treating obesity contains the extract as an active ingredient. [Reference numerals] (AA) Epididymis fat; (BB) Epididymis fat weight(g); (CC) Angelica Keiskei Koid. juice; (DD) Angelica Keiskei Koid. meal extract powder

Description

Composition for preventing and treating obesity comprising Angelica Keiskei Koid. extract}

The present invention relates to a composition for the prevention and treatment of obesity, including the extract of Mt. It relates to a composition for.

Obesity caused by energy intake and imbalance is known to be caused by various causes, including genetic factors, high-fat-oriented eating habits, environmental factors including lack of exercise, smoking and heavy drinking, and psychological factors caused by stress. Recently, the obesity population is rapidly increasing due to excessive calorie intake and lack of exercise due to high fat and high carbohydrate intake, and it is a social problem due to the increasing prevalence of childhood obesity as well as adults. Obesity is not only a problem in itself, but also a cause of many diseases such as hyperinsulinemia, arteriosclerosis, and cardiovascular disease, and thus, much attention has been drawn to obesity treatment worldwide (Nature, 404: 635-643, 2000; JAMA, 282: 1523-1529, 1999). Among the most known obesity treatments to date, Reductil ^TM (Abbott, USA), Xenical ^TM (Roche Pharmaceuticals, Switzerland), Exolise ^TM (Atopama, France), etc. It has been reported to cause serious side effects such as respiratory diseases and nervous system diseases. Various studies are currently being conducted to develop obesity treatments, and research is being actively conducted with approaches such as suppressing appetite, suppressing caloric absorption, promoting exothermic reactions, regulating energy metabolism, and enhancing basic metabolism through mitochondrial biosynthesis (Nature 404 , 635-643, 2000). Attempts have been made to search for new materials and drugs exhibiting multiple functions for these various approaches, but until now, the development of drugs having both safety and complex efficacy has been insufficient. Therefore, the attempt to find a component that matches the obesity treatment strategy from natural products and use it as a drug would be an efficient approach in terms of utilizing materials that have been proven safe and consumed as food, compared to developing therapeutic agents from synthetic agents.

Recently, research on the search for new functional anti-obesity materials derived from natural products and active use of foods have been actively conducted, among them, green juice containing a large amount of various bioactive substances such as antioxidant, anti-obesity, and lipid lowering along with the domestic well-being hot air. Interest is on the rise. Currently, green juice products using various fruits and vegetables such as Myeongil Leaf, Parsley, Carrot, etc. are being sold, and the production of juice by-products generated during the manufacture of green juice is increasing along with the production of green juice. Since these by-products are currently being used or disposed of as feed, the necessity for using the by-products is increasing.

Accordingly, the present inventors, while conducting research to search for natural substances that have excellent anti-obesity activity and can be safely applied, by using Myeong-Il-Pak, which is a by-product generated after the juice of green juice of Angelica Keiskei Koid. From this, the extract was prepared by applying a purification method to remove the water-soluble component, and the extract was confirmed to excellent weight loss effect, body fat reduction and fat cell size reduction effect was completed the present invention.

Accordingly, it is an object of the present invention to provide a composition for preventing and treating obesity, including the extract of Mt.

In order to achieve the object of the present invention as described above, the present invention provides a food composition for the prevention and treatment of obesity comprising the extract of Mt.

In order to achieve another object of the present invention, the present invention provides a pharmaceutical composition for the prevention and treatment of obesity, including the extract of Mt.

In order to achieve another object of the present invention, the present invention

(a) preparing an organic solvent extract of perilla leaf gourd;

(b) removing water-soluble components by adding water to the extract of step (a); And

(c) adding an alcohol having 1 to 6 carbon atoms to the extract from which the water-soluble component of step (b) is removed to obtain an alcohol soluble fraction. Provide a water-insoluble fraction.

The present invention is described in detail below.

The composition of the present invention includes the extract of Myeongil Leaf Park as an active ingredient, has an excellent preventive and therapeutic effect against obesity.

In the present invention, Myeongil leaf is the by-product from the juice of Myeongil leaf. The juice is a process of separating the juice from the solid or semi-flowable raw material, for example, crushing the object and then squeezed with a compress. Light leaf leaves show residual solids after juice.

Myeongil leaf extract of the present invention is preferably an organic solvent extract of Myeongil leaf is its water-insoluble fraction.

Preferably Myeongil leaf extract of the present invention

(a) preparing an organic solvent extract of perilla leaf gourd; And

(b) It may be prepared by a method comprising the step of removing water-soluble components by adding water to the extract of step (a).

The detailed description of each step is as follows.

Step (a) is a step of preparing an organic solvent extract of Myeongil Leaf Park.

In this step, the day leaf can be extracted by a solvent extraction method known in the art. Extraction solvents include, but are not limited to, alcohols having 1 to 6 carbon atoms, such as methanol, ethanol, propanol, isopropanol, butanol, pentanol, hexanol, acetone, ethyl acetate, n-nucleic acid, diethyl ether, acetone , Any one selected from organic solvents such as benzene, or a mixed solvent thereof may be used. Preferably it can be extracted with an alcohol having 1 to 6 carbon atoms or a mixed solvent thereof, more preferably ethanol can be extracted with a solvent.

In one embodiment of the present invention was extracted using ethanol as a solvent, in order to improve the yield 100% ethanol was used as the solvent. Extraction time is not limited thereto, but may be extracted for 1 to 20 hours, preferably for 10 to 15 hours, more preferably for 12 to 13 hours. In addition, the extraction temperature is not limited to this, but may be extracted at 25 ℃ to 75 ℃, more preferably 50 to 60 ℃ can be extracted, but above 75 ℃ the stability is difficult to perform the extraction process is preferred In this case, the extraction efficiency is lower than 25 ° C., which is not preferable.

Step (b) is a step of removing water-soluble components by adding water to the extract of step (a).

In this step, the water-soluble component is removed to obtain a water-insoluble precipitate. The method for removing the water soluble component may be by a method known in the art, for example, by adding an excess of water and stirring to remove the water soluble component in water. In addition, water may be added and the water-soluble component may be removed during the purification thereof, that is, the present invention is not limited thereto, but may be removed during separation using a filter paper, separation using an ultrafiltration membrane, separation by chromatography, and decolorization. In addition, it can be carried out according to the method of filtration with a Chiso filter to obtain a precipitate, which is mixed with water again and filtered again.

Myeongil leaf extract of the present invention may be prepared by a method further comprising the step of (c) 추출물 to obtain an alcohol dissolution fraction by adding an alcohol having 1 to 6 carbon atoms to the extract from which the water-soluble component of step (b) is removed. have.

In other words, Myeongil foliar extract of the present invention

(a) preparing an organic solvent extract of perilla leaf gourd;

(b) removing water-soluble components by adding water to the extract of step (a); And

(c) The extract from which the water-soluble component of step (b) is removed may be prepared by a method comprising adding an alcohol having 1 to 6 carbon atoms to obtain an alcohol soluble fraction.

Steps (a) and (b) are as described above, and step (c) is performed by adding an alcohol having 1 to 6 carbon atoms to the extract from which the water-soluble component of step (b) is removed to obtain an alcohol soluble fraction. Step.

The addition of alcohol increases the content of the active ingredient of the present invention, there is an effect that can additionally remove other inactive ingredients. The alcohol is preferably an alcohol having 1 to 6 carbon atoms, more preferably methanol, ethanol, propanol, butanol, pentanol, hexanol, and most preferably ethanol. The alcohol may be in a form in which a small amount of water (eg, 95% ethanol, 99% ethanol) is added to reflect physicochemical properties such as hygroscopicity. The treatment temperature and time are not particularly limited as long as the purpose of removing the inactive components is achieved, but may be treated at 30 to 50 ° C. for 30 minutes to 12 hours.

In addition, by removing the water-soluble substance of the step (b) and the additional treatment of the alcohol of the step (c) of the extract of the Myeongil leaf extract of the present invention has the effect of increasing its utility.

On the other hand, the concentration and purification process, filtration process, drying process, freezing process, powdering process may be optionally added for ease of treatment, storage and the like during the preparation of the extract of Myeongil foliar extract of the present invention.

The concentration process is not limited thereto, but may be concentrated using precipitation concentration, evaporation concentration, reduced pressure concentration, ultrafiltration, reverse osmosis, and centrifugal separation, and the filtration process is not limited thereto. Separation and separation funnels may be used. The drying process is not limited thereto, but may be freeze drying or spray drying, and may be powdered by a process such as dextrin inclusion.

In one embodiment of the present invention to establish the effective conditions for the extraction of Myeongil leaf, in another embodiment of the present invention on the basis of this to prepare a non-aqueous fraction of Myeongil leaf extract, more specifically ethanol extract of .

In the experimental example of the present invention, the extract of Myeongil foliar extract prepared above was administered to an obese model mouse to confirm the prevention and treatment of obesity. As a result, it was confirmed that there is an excellent obesity prevention / treatment effect as compared to the non-administered control group and daylight juice administration control group (Fig. 1). In addition, it showed an excellent body fat reduction effect (Fig. 2), it was confirmed that the size of fat cells is also reduced (Fig. 3).

The composition of the present invention has an excellent obesity prevention and treatment effect. Therefore, the present invention provides a food composition for the prevention and treatment of obesity, including the extract of Mt.

The food composition of the present invention includes all forms such as functional food, nutritional supplement, health food and food additives.

Food compositions of this type can be prepared in various forms according to conventional methods known in the art. For example, but not limited to, health food can be consumed by liquefaction, granulation, encapsulation and powdered so that the extract of Myeongil foliar extract in the form of tea, juice and drinks to drink. In addition, it can be prepared in the form of a composition by mixing with Myeongil leaf extract and known active ingredients known to have an effect on obesity. In addition, functional foods include, but are not limited to, beverages (including alcoholic beverages), fruits and processed foods (e.g. canned fruit, canned food, jams, marmalade, etc.), fish, meat and processed foods (e.g. ham) , Sausage cornbeans, etc., breads and noodles (e.g. udon, soba noodles, ramen noodles, spaghetti, macaroni, etc.), fruit juices, various drinks, cookies, candy, dairy products (e.g. butter, cheese, etc.), edible vegetable oils, margarine , Vegetable protein, retort foods, frozen foods, various seasonings (eg, miso, soy sauce, sauce, etc.) may be prepared by adding Myeongil leaf extract. In addition, in order to use the extract of the day single leaf in the form of food additives can be prepared in the form of powder or concentrate.

Preferable content of the extract of Mt. Foliage in the food composition of the present invention is not limited thereto, but is preferably 0.1 to 90% by weight of the finally prepared food. More preferably, the food composition containing the extract of Myeong il bak leaf of the present invention as an active ingredient may be prepared in the form of health food, especially by mixing with active ingredients known to be effective in obesity.

In addition, the present invention provides a pharmaceutical composition for the prevention and treatment of obesity, including the extract of Mt.

In the case of the pharmaceutical composition may include the extract of Sunil leaf alone or may further include one or more pharmaceutically acceptable carriers, excipients or diluents. As used herein, 'pharmaceutically acceptable' refers to a composition that is physiologically acceptable and does not normally cause an allergic or similar reaction when administered to a human.

The pharmaceutically acceptable carrier may further include, for example, a carrier for oral administration or a carrier for parenteral administration. Carriers for oral administration may include lactose, starch, cellulose derivatives, magnesium stearate, stearic acid, and the like. In addition, carriers for parenteral administration may include water, suitable oils, saline, aqueous glucose and glycols, and the like, and may further include stabilizers and preservatives. Suitable stabilizers include antioxidants such as sodium hydrogen sulfite, sodium sulfite or ascorbic acid. Suitable preservatives include benzalkonium chloride, methyl- or propyl-paraben and chlorobutanol. Other pharmaceutically acceptable carriers can be found in Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Company, Easton, Pa., 1995).

The pharmaceutical composition for preventing or treating obesity of the present invention can be administered to any mammal, including humans. For example, it can be administered orally or parenterally. Parenteral administration methods include, but are not limited to, intravenous, intramuscular, intraarterial, intramedullary, intradural, intracardiac, transdermal, subcutaneous, intraperitoneal, intranasal, intestinal, topical, sublingual or rectal administration. Can be. Preferably the pharmaceutical composition of the present invention may be administered transdermally. As used herein, 'transdermal administration' refers to the administration of the pharmaceutical composition of the present invention to cells or skin to deliver the active ingredients contained in the pharmaceutical composition for the prevention or treatment of obesity into the skin. For example, the pharmaceutical composition of the present invention can be prepared in an injectable formulation and administered by lightly pricking the skin with a 30 gauge thin needle or by applying it directly to the skin.

In addition, the pharmaceutical composition may be formulated into a preparation for oral or parenteral administration according to the route of administration as described above.

In the case of preparations for oral administration, the compositions of the present invention may be formulated using methods known in the art as powders, granules, tablets, pills, dragees, capsules, solutions, gels, syrups, slurries, suspensions and the like. Can be. For example, oral formulations can obtain tablets or dragees by combining the active ingredients with solid excipients and then grinding them, adding suitable auxiliaries and processing them into granule mixtures. Examples of suitable excipients include, but are not limited to, sugars including lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol and maltitol, and starches including corn starch, wheat starch, rice starch and potato starch, Cellulose such as methylcellulose, sodium carboxymethylcellulose and hydroxypropylmethyl-cellulose and the like, fillers such as gelatin, polyvinylpyrrolidone and the like. In addition, crosslinked polyvinylpyrrolidone, agar, alginic acid or sodium alginate and the like may optionally be added as a disintegrant. Furthermore, the pharmaceutical composition of the present invention may further include an anticoagulant, a lubricant, a humectant, a perfume, an emulsifier, a preservative, and the like.

Formulations for parenteral administration may be formulated by methods known in the art in the form of injections, creams, lotions, external ointments, oils, moisturizers, gels, aerosols and nasal inhalants. These formulations are described in Remington's Pharmaceutical Science, 15th Edition, 1975. Mack Publishing Company, Easton, Pennsylvania 18042, Chapter 87: Blaug, Seymour, a prescription generally known in all pharmaceutical chemistries.

The total effective amount of the extract of the day leaf extract of the present invention may be administered to a patient in a single dose, and may be administered by a fractionated treatment protocol administered for a long time in multiple doses. . The pharmaceutical composition of the present invention may vary the content of the active ingredient depending on the extent of the disease. Preferably the preferred total dose of the extract of Myeongil Foil is from about 0.01 μg to 1,000 mg, most preferably 0.1 μg to 100 mg per kg of patient body weight per day. However, the dose of the extract of Myeongil leaf extract is effective dose to the patient in consideration of various factors such as the age, weight, health condition, sex, severity of the disease, diet and excretion rate, as well as the route and frequency of treatment of the pharmaceutical composition In this regard, one of ordinary skill in the art will be able to determine an appropriate effective dosage for the specific use as a prophylactic or therapeutic agent for obesity. The pharmaceutical composition according to the present invention is not particularly limited to its formulation, route of administration and method of administration as long as the effect of the present invention is shown.

Furthermore, the pharmaceutical composition may further include an anticoagulant, a lubricant, a humectant, a perfume, an emulsifier, a preservative, and the like.

Formulations for parenteral administration may be formulated in the form of injections by methods known in the art. These formulations are described in Remington's Pharmaceutical Science, 15th Edition, 1975. Mack Publishing Company, Easton, Pennsylvania 18042, Chapter 87: Blaug, Seymour, a prescription generally known in all pharmaceutical chemistries.

As described above, the Myeongil leaf extract of the present invention has an excellent weight loss, body fat reduction and fat cell size reduction effect compared to the juice of the original Myeongil leaf. Therefore, Myeongil leaf extract of the present invention can be usefully used for the purpose of preventing and treating obesity.

1 is a normal group (hereinafter referred to as 'Normal'), a high fat diet control group (hereinafter referred to as 'Control'), Myeongil leaf juice administration group (hereinafter referred to as 'day leaf juice'), Myeongil leaf extract administration group (hereinafter 'Myeongil leaf leaf' It shows the result of measuring the weight change according to the administration).
Figure 2 shows the results of measuring the change in the body fat content by administration of the extract from the leaves of obesity in obese mice induced by high fat diet.
Figure 3 shows the results of measuring the change in fat cell size when administered Myeongil leaf extract in obese mice induced by high fat diet.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

≪ Example 1 >

Setting Optimum Conditions for Preparation of Mt.

The experiment was carried out as follows to set the optimum conditions for the preparation of Mt. In the present invention, the raw material of Myeongil leaf was used as a by-product (myeongil leaf gourd) of the remaining solid components of the juice of Myeongil leaf.

1. Extraction solvent, time and temperature

In order to compare the extraction efficiency according to the extraction solvent, extracted with purified water, 30% ethanol, 60% ethanol and 100% ethanol at 80 ℃ for 3 hours. Solid content content) was compared and the calculated yield was found to be very low. In the case of ethanol extraction, extraction with 100% ethanol showed the highest efficiency.

In order to compare the extraction efficiency according to the extraction time, the extraction time was extracted as 5 hours, 10 hours, 15 hours and 20 hours. As a result, the extraction efficiency increased the most between 10 and 15 hours. Therefore, the extraction time was set at 1 hour interval from 10 hours to 15 hours and compared. As a result, the extraction was the most efficient in the case of extraction for 12 to 13 hours.

In order to compare the extraction efficiency according to the extraction temperature, the extraction temperature was extracted at room temperature, 25 ℃ and 75 ℃. As a result, the solid content increased as the temperature increased, but the process was difficult because the stability was lower than 75 ℃. 50 ° C. to 60 ° C. has been found to be suitable.

2. Concentration and Filtration

In order to establish the most efficient concentration concentration, the extracted extract was concentrated at 1/2 ratio, 1/5 ratio and at maximum ratio. As a result, as the concentration increased, the solids also increased, but the loss of recovery occurred at the maximum concentration.

In order to optimize the filtration process for recovering the active ingredient, filtration using a 20 mesh filter network or a micro filter, filtration using a centrifugation separator and a funnel was performed. As a result, in case of centrifugation, the recovery efficiency is high, but a large amount of loss occurs during recovery, and the separation funnel has a long separation time, a small capacity, and difficult separation. In the case of using the filter network, the 20 mesh filter network has a low recovery rate, and when the micro filter is used, the recovery rate is good and the working efficiency is high.

3. Separation and Purification

The extract extracted with ethanol was concentrated under reduced pressure, and the insoluble precipitate was recovered and dried, dissolved in alcohol, filtered through a filter paper and concentrated to dryness to obtain a water-insoluble component. The water-soluble and water-insoluble components were separated by the method.

The extraction solvent may be any one selected from alcohols having 1 to 6 carbon atoms such as water, ethanol and methanol, organic solvents such as acetone, ethyl acetate, n-nucleic acid, diethyl ether, acetone, benzene, or a mixed solvent thereof. Can be used. The extract is concentrated using a concentrator known in the art, a concentration method, and the precipitate is separated using a filter paper to remove the water-soluble components from the concentrate, separation using an ultrafiltration membrane, separation by various chromatography, decolorization Purification process such as process was applied. Finally, the concentrated solution from which the water-soluble component was removed was powdered by a method such as lyophilization, spray drying, and dextrin inclusion. However, the extraction and separation and purification method of the active ingredient is not necessarily limited to the above method.

<Example 2>

Preparation of sample

<2-1> Myung-Yeop Juice  Produce

In order to compare the anti-obesity effect of the extract of Myeongil-yeop and its by-products, juice was prepared from Myeongil-yeop first by the following method.

500g of light days were squeezed to obtain juice. For storage and later experiments, the freeze-dried powder was prepared by freezing at -80 ° C. for 1 day and then drying it by sublimation to directly ice the ice by lowering the partial pressure of water vapor.

<2-2> Leaf leaf  Preparation of extract

After the extract of Myeongil lobe, according to the conditions set in Example 1 using the by-product Myeongil lobe, Myeongil lobe extract was prepared by the following method. 300 g of ethanol was added to 350 g of the by-product after extracting the juice of the green leaf, followed by extraction at 55 ° C. for 12 hours. Filtering the extract from 1mesh to 150mesh in the conveying tube while transferring the extract through the conveying tube, and then condensing it by 40 brix at low temperature using an evaporator to remove ethanol and then 55 ° C. until 60 brix. Concentrated at.

The precipitated component was passed through the Chiso filter at a rate of 85 ml / min, and the precipitate was filtered and recovered. The recovered immersed component was dehydrated at 85 ° C. for 6 hours to remove moisture.

In order to increase the content of the non-aqueous component in the recovered precipitate, 10 times of water of the precipitate was added and stirred at 80 ° C. for 2 hours. After cooling, the water was removed to remove the water-soluble portion. Lyophilized for later treatment.

In addition, in order to further increase the water-insoluble component of the extract, ethanol (95%) was further added to 15 times the precipitate, and the solution was stirred at 40 ° C. for 1 hour.

It was concentrated under reduced pressure and freeze-dried and used in the following animal experiments.

< Experimental Example  1>

Leaf leaf  Weight loss effect of extract

In order to confirm the weight loss effect of the extract of Myeongil foliar extract of the present invention prepared in Example 2, an experiment was performed by selecting a mouse model inducing obesity with a high fat diet (J Korean Soc Food Sci Nutr .39 (10) , 1439-1445 (2010).

Five-week-old C57BL / 6 mice were acclimated for one week and fed with a high-fat diet for eight weeks to induce obesity to induce obesity, and eight mice each were randomly divided into four groups and used in the experiment. The experimental group was divided into four groups as follows, and administration was performed once a day by oral administration, and the extracts of the extract of Myeongil-day juice and Myeongil-dayak were each administered 0.3g / 60kg in terms of body weight. Experimental Group 1 (normal diet, group shown below as normal), Experimental Group 2 (high fat dietary control, as shown below Control), Experimental Group 3 (high-fat diet and daily juice extract group), Experimental group 4 (high-fat diet and sunday extract) Powder group) to test. Each subject was weighed every week for a total of eight weeks.

As a result, as shown in Figure 1, the high-fat diet control group measured 8 weeks after the sample administration showed a weight gain of about 77.8% compared to the initial weight, the juice extract of the day of the week showed a weight increase rate of 67.8%. Compared to the control group, the extract of Myeongil-yup extract showed a 45.7% increase, and the weight gain rate was significantly lower than that of the control group (* p <0.05). When comparing the weight gain rates of the extracts of the extract of the extract of the extract of the extract from the extract of the extract of the extract of the extract from the extract of the extract of the extract of the extract of the extract from the extract of the extract of the extract of the extract of the extract from the extract of the extract, the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract from the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract containing the extract of the extract prepared with the extract of the extract, the extract of the extract of the extract of the extract of the extract, From these results, it was confirmed that the anti-obesity effect of the extract of Myeongil foliar extract that increased the water-insoluble component by extracting, concentrating, and purifying the byproduct of Myeonil.

< Experimental Example  2>

Leaf leaf  Body Fat Reduction Effect of Extracts

Obesity mice induced by the high fat diet of Experimental Example 1 were sacrificed after fasting for at least 12 hours after the end of 8 weeks of administration. The abdomen of the sacrificed mice was incised, and then the epididymal fats were extracted and washed with physiological saline to remove water and weighed.

As a result, as shown in Figure 2, it can be seen that the weight of the epididymal fat increased by more than four times compared to the normal group according to the high-fat diet, the day juice extract group was reduced by about 17.4% compared to the control group. In comparison, the extracts of the extract of Myeongil Lobe extract showed a significant decrease rate of about 31.4% (* p <0.05), indicating that the epididymal fat accumulation inhibitory effect of the extract of Myeongil Lobe extract was about 2 times higher than that of juice. From these results, it was confirmed that the extract of Myeongil foliar extract, in which the water-insoluble component of the present invention, was concentrated, compared to the extract of Myeongil berry juice.

< Experimental Example  3>

Leaf leaf  Changes in Fat Cell Size of Extracts

Obesity mice induced by the high fat diet of Experimental Example 1 were sacrificed after fasting for at least 12 hours after the end of 8 weeks of administration. The abdomen of the mouse was excised and subcutaneous fat was quickly removed and soaked in 4% formaldehyde solution. After fixation and washing with water and dehydration, the solution was treated with a paraffin solution to form a paraffin block, cut into 4μm thicknesses, stained with hematoxylin and eosin, and observed under an optical microscope. .

As a result, as shown in Figure 3, it was confirmed that the adipose tissue of the extract of Myeongil lobe extract powder was significantly reduced compared to the extract of Myungil lobe juice. Therefore, it was confirmed that the fat cell size reduction effect of the extract of Myeongil Leaf was superior to that of Myungil Leaf juice.

Myeongil leaf extract of the present invention has an excellent weight loss, body fat reduction and fat cell size reduction effect compared to the juice of the original Myeongil leaf. Therefore, Myeongil leaf extract of the present invention can be usefully used for the purpose of preventing and treating obesity.

Claims (6)

Food composition for the prevention and treatment of obesity, including the extract of Mt.
The composition of claim 1, wherein the extract of Myeongil Foil is a non-aqueous fraction of the organic solvent extract of Myeongil Foil.
The extract of claim 1, wherein
(a) preparing an organic solvent extract of perilla leaf gourd; And
(b) a composition prepared by the method comprising the step of removing water-soluble components by adding water to the extract of step (a).
The extract of claim 1, wherein
(a) preparing an organic solvent extract of perilla leaf gourd;
(b) removing water-soluble components by adding water to the extract of step (a); And
(c) a composition comprising the step of obtaining an alcohol soluble fraction by adding an alcohol having 1 to 6 carbon atoms to the extract from which the water-soluble component of step (b) is removed.
The solvent according to any one of claims 2 to 4, wherein the organic solvent is one selected from the group consisting of alcohols having 1 to 6 carbon atoms, acetone, ethyl acetate, n-nucleic acid, diethyl ether, acetone and benzene. The composition characterized in that the.
Pharmaceutical composition for the prevention and treatment of obesity, including the extract of Mt.

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