KR20120086087A - Polymerase Chain Reaction system, its thermal control apparatus and its thermal control method - Google Patents

Abstract

PURPOSE: A gene amplification system and a temperature control device thereof are provided to largely improve uniformity and accuracy. CONSTITUTION: A gene amplification system(100) comprises: a sample container(2) containing a genetic sample(1); a temperature control device(10) for heating and cooling the sample container to control the temperature of the genetic sample; and an optical device(20) for sensing the optical change of the genetic sample according to the temperature. The temperature control device comprises: a heat exchange block contacting the sample container; a first heat medium supply(11) for supplying a thermal medium(M1) of first temperature to the heat exchange block; a second thermal medium supply(12) for supplying thermal medium(M2) of second temperature to the heat block; a third thermal medium supply(13) for supplying thermal medium(M3) of third temperature to the heat exchange block; and a control unit(14) for authorizing control signals to the first, second, and third thermal medium supplies.

Description

Genetic amplification system, temperature control device and temperature control method of gene amplification system {Polymerase Chain Reaction system, its thermal control apparatus and its thermal control method}

The present invention relates to a gene amplification system, a temperature control device and a temperature control method of the gene amplification system, and more particularly, to overshoot (under shooting) or under shooting phenomenon during the temperature control of the gene sample. The present invention relates to a gene amplification system, a temperature control device, and a temperature control method of a gene amplification system, which can be prevented to greatly improve the reliability, accuracy, and uniformity of equipment.

Polymerase Chain Reaction (PCR) is a type of DNA amplification that uses DNA polymerase to amplify large amounts of DNA with only a small amount of gene samples to perform gene research, experiments, and microbial detection. It is widely used as a gene amplification method.

In order to perform PCR, which is a gene amplification method, a gene amplification device having a temperature control device for adjusting the temperature of the gene sample and an optical device for detecting an optical change according to the temperature of the gene sample have been developed.

In particular, in order to facilitate gene amplification, a temperature control process is performed in which a gene sample is heated to a precise temperature rapidly over time, or a series of processes for cooling the gene sample to a precise temperature quickly over time is very important. It is important.

Conventionally, in order to control the temperature, a thermoelectric element such as a peltier device is directly attached to a thermal contact block in thermal contact with the gene sample so as to heat and cool the gene sample, thereby controlling the temperature of the gene sample.

However, when the gene sample is to be heated quickly to the desired temperature using the Peltier device, ie at a rate of 1.0 to 2.5 degrees C / sec (° C / sec) per second, the desired temperature is shown, as shown in FIG. The phenomenon of overheating, or overshooting, occurs, which causes the actual temperature to fluctuate at an amplitude of up to approximately 3.5 degrees in a wave shape, and then it is required to flatten to the desired temperature as the amplitude decreases. Time for plateau (about 40 seconds in FIG. 1) is generated.

In addition, when a gene sample is to be cooled quickly to a desired temperature using a Peltier device, that is, for example, at a rate of 1.0 to 2.5 degrees Celsius (° C / sec) per second, as shown in FIG. Subcooling below the temperature, or under shooting, occurs, which causes the actual temperature to fluctuate at an amplitude of up to approximately 3.5 degrees in a wave shape and then flatten to the desired temperature as the amplitude decreases. Time for plateau (about 30 seconds in FIG. 2) is also generated.

Therefore, short DNA as well as long DNA having a high failure rate during a time for plateau due to overshooting or undershooting are required. Gene amplification also failed, or the disassembly and re-copy of the gene chain did not occur smoothly, greatly reducing the reliability and accuracy of the result, there was a problem that the uniformity also greatly reduced during repeated experiments.

The technical problem to be achieved by the present invention is to prevent overshooting and undershooting to facilitate the disassembly and re-copy of the gene chain, greatly improve the reliability and accuracy of the results of the equipment, and also uniformity during repeated experiments The present invention provides a gene amplification system, a temperature control device and a temperature control method of the gene amplification system, which can be greatly improved and can greatly improve the precision of the equipment, thereby enabling the production of high performance gene amplification equipment.

Gene amplification system according to the present invention for achieving the above technical problem, the sample stand on which the gene sample is seated; A temperature control device for heating and cooling the sample stage to adjust the temperature of the gene sample; And an optical device for detecting an optical change according to the temperature of the gene sample, wherein the temperature control device comprises: a heat exchange block in thermal contact with the sample stage; A first heat medium supply device for supplying a heat medium of a first temperature to the heat exchange block; A second heat medium supply device for supplying a heat medium of a second temperature to the heat exchange block; A third heat medium supply device for supplying a heat medium of a third temperature to the heat exchange block; And the first heat medium supply device to sequentially supply the heat medium at the first temperature, the heat medium at the second temperature, and the heat medium at the third temperature to the heat exchange block according to a series of programs or commands. And a control unit for applying a control signal to the second thermal medium supply device and the third thermal medium supply device.

Further, according to some embodiments of the invention, the first temperature is a heating temperature to disassemble both male and female chains of the gene, and the second temperature is a cooling temperature to anneal both male and female chains of the disassembled gene. The third temperature may be a reheating temperature at which the annealed male and female chains replicate themselves and their corresponding chains, respectively.

The first temperature according to the present invention is in the range of 90 degrees to 100 degrees Celsius, the second temperature is in the range of 50 degrees to 70 degrees, and the third temperature is in the range of 70 degrees to 80 degrees Celsius. It is possible.

In addition, the first thermal medium supply apparatus according to the present invention, the first thermal medium storage for receiving a thermal medium of the first temperature; A first temperature holding device installed in the first thermal medium storage and maintaining the first thermal medium at a first temperature by using a first temperature sensor, a first temperature holding control unit and a heater; A circulation line connecting the first heat medium reservoir to the heat exchange block and circulating the heat medium; A first valve installed in the circulation line and regulating the circulation of the thermal medium at the first temperature; And a first pump installed in the circulation line and circulating the heat medium having the first temperature.

In addition, the optical device according to the invention, the light source for generating and providing light; A first filter for converting light generated from the light source; A light guide member for guiding the light converted by the first filter toward the gene sample and guiding the reaction light of the gene sample; A second filter for converting the reaction light; And an analyzer for analyzing the reaction light converted by the second filter.

The temperature control device of the gene amplification system according to the present invention for achieving the above technical problem, in the configuration of the temperature control device of the gene amplification system for heating and cooling the sample stage to control the temperature of the gene sample, A heat exchange block in thermal contact with the stage; And a first heat medium supply device for supplying a heat medium at a first temperature to the heat exchange block, wherein the first heat medium supply device comprises: a first heat medium storage unit for receiving a heat medium at a first temperature; A first temperature holding device installed in the first thermal medium storage and maintaining the thermal medium at a first temperature by using a first temperature sensor, a first temperature holding control unit and a heater; A circulation line connecting the first heat medium reservoir and the heat exchange block; A first valve installed in the circulation line and regulating the circulation of the thermal medium at the first temperature; And a first pump installed in the circulation line and circulating the heat medium having the first temperature.

In addition, the temperature control device of the gene amplification system according to some embodiments of the present invention, the second heat medium supply device for supplying a heat medium of a second temperature to the heat exchange block; further comprising the second heat medium The supply apparatus comprises: a second thermal medium reservoir for receiving a thermal medium at a second temperature; A second temperature holding device installed in the second thermal medium storage and maintaining the thermal medium at a second temperature by using a second temperature sensor, a second temperature holding control unit and a heater; A circulation line connecting the second heat medium reservoir to the heat exchange block; A second valve installed in the circulation line and regulating the circulation of the thermal medium at the second temperature; And a second pump installed in the circulation line and circulating the heat medium having the second temperature.

In addition, the temperature control device of the gene amplification system according to an embodiment of the present invention, further comprises a third heat medium supply device for supplying a heat medium of a third temperature to the heat exchange block, the third heat medium The supply apparatus includes: a third thermal medium reservoir for receiving a thermal medium at a third temperature; A third temperature holding device installed in the third thermal medium storage and maintaining the thermal medium at a third temperature by using a third temperature sensor, a third temperature holding control unit and a heater; A circulation line connecting the third heat medium reservoir to the heat exchange block; A third valve installed in the circulation line and regulating the circulation of the thermal medium at the third temperature; And a third pump installed in the circulation line and circulating the heat medium having the third temperature.

In addition, the first valve, the second valve and the third valve according to the present invention are an input valve package having an input of 3 way and an output of 1 way, and an input of 1 way, The valve assembly may be a three-way outlet valve package.

The temperature control method of the gene amplification system according to the present invention for achieving the above technical problem, in the temperature control method of the gene amplification system for heating and cooling the sample stand on which the gene sample is seated, heat exchange in thermal contact with the sample stand Supplying the block with a thermal medium at a first temperature stored in a first thermal medium reservoir; Supplying a heat medium of a second temperature stored in a second heat medium reservoir to the heat exchange block; And supplying a heat medium having a third temperature stored in a third heat medium storage to the heat exchange block.

The gene amplification system and the temperature control device and the temperature control method of the gene amplification system of the present invention can greatly improve the reliability and accuracy of the results of the equipment, greatly improve the uniformity during repeated experiments, and greatly improve the precision of the equipment By improving and having the effect of producing a high performance gene amplification equipment.

1 is a graph showing an overshooting phenomenon of a conventional gene amplification apparatus.
2 is a graph showing an undershooting phenomenon of a conventional gene amplification apparatus.
3 is a conceptual diagram illustrating a gene amplification system according to an embodiment of the present invention.
4 is an external perspective view of a gene amplification system according to an example of FIG. 3.
5 is a plan view from which the case of FIG. 4 is removed.
6 is a side view of FIG. 5.
FIG. 7 is a side view illustrating a sample stand drawing state of FIG. 6. FIG.
FIG. 8 is a side view illustrating a scan state of the optical device of FIG. 7.
9 is a photograph showing an example of a temperature control device of the gene amplification system of FIG.
10 is a conceptual diagram illustrating another example of the temperature control device of the gene amplification system of FIG. 3.
11 is an exploded perspective view of parts illustrating an example of FIG. 10.
12 is a block diagram showing a temperature control method of a gene amplification system according to an embodiment of the present invention.
13 is a temperature graph according to the temperature control method of the gene amplification system of FIG.

Hereinafter, various exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. The embodiments of the present invention are described in order to more fully explain the present invention to those skilled in the art, and the following embodiments may be modified into various other forms, It is not limited to the embodiment. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. In addition, the thickness or size of each layer in the drawings is exaggerated for convenience and clarity of description. Like numbers refer to like elements herein. Throughout the specification, when referring to one component, such as a layer, region, or substrate, being located on, “connected”, or “under” another component, the one component is directly in another configuration. It may be interpreted that there may be other components in contact with or interposed between, or “on,” “connected”, or “under” an element. On the other hand, when one component is referred to as being located on another component "directly on", "directly connected", or "directly under", it is interpreted that there are no other components intervening therebetween. do.

3 is a conceptual diagram illustrating a gene amplification system according to an embodiment of the present invention, FIG. 4 is an external perspective view of the gene amplification system according to an example of FIG. 3, FIG. 5 is a plan view from which the case of FIG. 4 is removed, and FIG. 6. 5 is a side view of FIG. 5, FIG. 7 is a side view showing a sample-to-retraction state of FIG. 6, FIG. 8 is a side view showing a scanning state of the optical device of FIG. 7, and FIG. 9 is a temperature control of the gene amplification system of FIG. 3. It is a photograph showing an example of an apparatus.

As shown in Figures 3 to 9, the gene amplification system 100 according to an embodiment of the present invention, a sample in which the gene sample 1 is placed inside the main body 101 and the case 102 of FIG. It is a configuration that can be made, including the table 2, the temperature control device 10, the optical device 20 and the sample table transfer device 30.

Here, the temperature control device 10 of FIG. 3 is a device for heating and cooling the sample stand 2 to adjust the temperature of the gene sample 1, and is in thermal contact with the sample stand 2. The heat exchange block B, the first heat medium supply device 11, the second heat medium supply device 12, the third heat medium supply device 13 and the control unit 14 may be made. .

As shown in FIG. 3, the first thermal medium supply device 11 supplies the thermal medium M1 having a first temperature to the heat exchange block B, and thus, the first thermal medium storage unit ( 111, the first temperature maintaining device 113, the circulation line L1, the first valve V1, and the first pump P1.

Here, the first thermal medium storage 111 is large enough to cause no overshooting or undershooting, that is, at least to circulate the heat exchange block B and the first thermal medium storage 111. It may be a kind of thermal medium storage tank for receiving the thermal medium M1 of the first temperature in a sufficient amount.

In one example, the first temperature may be in the range of 90 degrees Celsius to 100 degrees Celsius which is a heating temperature for dismantling both male and female chains of the gene.

In addition, as shown in FIG. 3, the first temperature holding device 113 is installed in the first thermal medium storage 111 and includes a first temperature sensor S1 and a first temperature holding control unit 112. And the heater H1 to maintain the thermal medium M1 at a first temperature.

Here, the first temperature sensor (S1) may be applied to a variety of temperature sensors using a bimetal, a thermoelectric phenomenon, a change in electrical resistance, etc., the heater (H1) is a common heating wire, a resistance rod or a Peltier using electric resistance heat Various heaters, such as an element, can be applied.

In addition, as illustrated in FIG. 3, the circulation line L1 connects the first heat medium storage 111 and the heat exchange block B, and a kind of heat through which the heat medium M1 is circulated. It acts as a media circulation pathway.

In addition, as shown in FIG. 3, the first valve V1 is installed in the circulation line L1, and various valves for controlling circulation of the thermal medium M1 at the first temperature may be applied. The first pump P1 is installed in the circulation line L1 and generates various hydraulic pressures of the internal heat medium M1 to circulate the heat medium M1 at the first temperature. Can be applied.

In addition, the second heat medium supply device 12, as shown in Figure 3, to supply the heat medium (M2) of the second temperature to the heat exchange block (B), the second heat medium storage ( 121, the second temperature maintaining device 123, the circulation line L2, the second valve V2, and the second pump P2.

Here, the second thermal medium reservoir 121 is large enough to cause no overshooting or undershooting, that is, at least to circulate the heat exchange block B and the second thermal medium reservoir 121. It may be a kind of thermal medium storage tank that receives a sufficient amount of thermal medium M2 at a second temperature.

In one example, the second temperature may be in the range of 50 to 70 degrees Celsius, which is a cooling temperature for annealing both male and female chains of the disassembled gene.

In addition, as shown in Figure 3, the second temperature holding device 123, which is installed in the second thermal medium storage 121, the second temperature sensor (S2) and the second temperature holding control unit 122. And the heater H2 to maintain the thermal medium M2 at the second temperature.

Here, the second temperature sensor S2 may be applied to various temperature sensors using a bimetal, a thermoelectric phenomenon, a change in electrical resistance, and the like, and the heater H2 may be a general heating wire, a resistance rod, or a Peltier using electric resistance heat. Various heaters, such as an element, can be applied.

In addition, as illustrated in FIG. 3, the circulation line L2 connects the second heat medium storage 121 and the heat exchange block B, and heat is sorted by the heat medium M2. It acts as a media circulation pathway.

In addition, as shown in FIG. 3, the second valve V2 is installed in the circulation line L2, and various valves for controlling circulation of the thermal medium M2 at the second temperature may be applied. The second pump (P2) is installed in the circulation line (L2), the various pumps for generating a hydraulic differential of the internal heat medium (M2) to circulate the heat medium (M2) of the second temperature Can be applied.

In addition, the third heat medium supply device 13, as shown in Figure 3, to supply the heat medium (M3) of the third temperature to the heat exchange block (B), the third heat medium storage ( 131, the third temperature maintaining device 133, the circulation line L3, the third valve V3, and the third pump P3.

Here, the third thermal medium storage 131 is sufficient to circulate the heat exchange block B and the third thermal medium storage 131 in an amount large enough to prevent overshooting or undershooting. It may be a kind of thermal medium storage tank for receiving the thermal medium M3 of the third temperature in a sufficient amount.

In one example, the third temperature may be in the range of 70 degrees to 80 degrees Celsius, which is the reheating temperature at which the annealed male and female chains replicate themselves and their corresponding chains, respectively.

In addition, as shown in FIG. 3, the third temperature holding device 133 is installed in the third thermal medium storage 131 and includes a third temperature sensor S3 and a third temperature holding control unit 132. And the heater H3 to maintain the thermal medium M3 at a third temperature.

Here, the third temperature sensor S3 may be applied to various temperature sensors using a bimetal, a thermoelectric phenomenon, a change in electrical resistance, and the like, and the heater H3 may be a general heating wire, a resistance rod, or a Peltier using electric resistance heat. Various heaters, such as an element, can be applied.

In addition, as illustrated in FIG. 3, the circulation line L3 connects the third heat medium storage 131 and the heat exchange block B, and heat is sorted by the heat medium M3. It acts as a media circulation pathway.

In addition, as shown in FIG. 3, the third valve V3 is installed in the circulation line L3, and various valves for controlling circulation of the thermal medium M3 at the third temperature may be applied. The third pump P3 is installed in the circulation line L3 and generates various hydraulic pressures of the internal heat medium M3 to circulate the heat medium M3 at the third temperature. Can be applied.

On the other hand, the control unit 14, the heat medium (M1) of the first temperature, the heat medium (M2) of the second temperature and the third temperature in the heat exchange block (B) according to a series of programs or commands. Control signals may be applied to the first thermal medium supply device 11, the second thermal medium supply device 12, and the third thermal medium supply device 13 so as to sequentially supply the thermal medium M3. It can be.

For example, the control unit 14 sequentially processes the first thermal medium supply device 11, the second thermal medium supply device 12, and the third thermal medium supply device 13 according to a series of programs. By applying a control signal to the heat exchange block (B) and supplying the heat medium (M1) of the first temperature for a predetermined time, and then supplying the heat medium (M2) of the second temperature for a predetermined time, A series of processes of sequentially supplying the thermal medium M3 at the third temperature can be repeatedly performed.

10 is a conceptual diagram which shows another example of the temperature control apparatus of the gene amplification system of FIG. 3, and FIG. 11 is an exploded perspective view of the component which shows an example of FIG.

According to another example of the temperature control device 10 of the gene amplification system 100 described above, as shown in Figs. 10 and 11, the first valve (V1), the second valve (V2) and the first The three-valve V3 includes an inlet valve package (IVP) having an input of three ways and an output of one way, and an output valve package (OVP) having an input of one way and an output of three ways ( A valve assembly (VA) comprising an outlet valve package may be applied.

That is, the input 3-way valve of the input valve package IVP serves as an input valve of the first valve V1, the second valve V2, and the third valve V3, respectively. The output one-way valve may serve as a main valve MV capable of integrally intermittently regulating the heat medium flowing toward the heat exchange block B.

In addition, the output side 3-way valve of the output valve package OVP serves as an output side valve of the first valve V1, the second valve V2, and the third valve V3, respectively. The one-way valve on the input side may serve as a main valve (MV) capable of integrally controlling the heat medium drawn from the heat exchange block (B). In addition, the input valve package IVP and the output valve package OVP may be changed and modified in various valve combinations without departing from the spirit of the present invention.

Therefore, when the operation of the temperature control device 10 of the gene amplification system 100 according to an embodiment of the present invention in more detail, as shown in FIG. 10, the control unit 14 of FIG. By the state in which both the main valve MV of the input valve package IVP and the main valve MV of the output valve package OVP are opened, the input side first valve of the input valve package IVP ( When the first valve V1 of V1 and the output side of the output valve package OVP is opened and the remaining second valve V2 and the third valve V3 are closed, the first thermal medium storage 111 The gene sample 1 of FIG. 3 is circulated through the heat exchange block B under the pressure of the first pump P1 and the heat medium M1 of the first temperature stored in a large quantity. It can be quickly heated to temperature. At this time, since a large amount of heat medium M1 of the first temperature is circulated swiftly after the heat exchange due to the nature of the heat medium circulation method, it is possible to quickly heat and cool at a desired speed without overshooting or undershooting. .

Subsequently, as shown in FIG. 10, the input side second valve V2 of the input valve package IVP and the output side second valve of the output valve package OVP are controlled by the control unit 14 of FIG. 3. When only V2 is opened and the remaining first valve V1 and the third valve V3 are closed, the thermal medium M2 of the second temperature that has been stored in a large amount in the second thermal medium storage 121. The gene sample 1 of FIG. 3 may be rapidly cooled to the second temperature while circulating the heat exchange block B under the pressure of the second pump P2. At this time, due to the nature of the heat medium circulation method, since a large amount of heat medium (M2) of the second temperature is circulated swiftly moving after the heat exchange, it is possible to quickly heat and cool at a desired speed without overshooting or undershooting. .

Subsequently, as shown in FIG. 10, the input side third valve V3 of the input valve package IVP and the output side third valve of the output valve package OVP are controlled by the controller 14 of FIG. 3. When only V3 is opened and the remaining first valve V1 and the second valve V2 are closed, the thermal medium M3 of the third temperature that has been stored in large quantities in the third thermal medium storage 131. The gene sample 1 of FIG. 3 may be rapidly reheated to a third temperature while circulating the heat exchange block B under the pressure of the third pump P3. At this time, due to the nature of the heat medium circulation method, since a large amount of the heat medium M3 of the third temperature is circulated swiftly after the heat exchange, it is possible to rapidly heat and cool at a desired speed without overshooting or undershooting. .

Here, the control unit 14 of FIG. 3 adjusts the opening / closing amount of the main valve MV of the input valve package IVP and the main valve MV of the output valve package OVP to adjust the thermal medium circulation amount. The amount of heat medium circulation may be adjusted by adjusting the pumping amounts of the first pump P1, the second pump P2, and the third pump P3.

On the other hand, when the input side of the first valve (V1), the second valve (V2) and the third valve (V3) of the input valve package (IVP) interrupts the main valve (MV), the heat medium is respectively the first heat medium A three-way valve connected to a separate return line may be applied to return to the reservoir 111, the second thermal medium storage 121, and the third thermal medium storage 131. These return lines are connected to the first valve V1, the second valve V2, and the third valve V3, respectively, and will be omitted in view of the complexity of the drawings.

12 is a block diagram illustrating a temperature control method of the gene amplification system according to an embodiment of the present invention, Figure 13 is a temperature graph according to the temperature control method of the gene amplification system of FIG.

That is, as shown in Figure 12, the temperature control method of the gene amplification system 100 for heating and cooling the sample stand 2, the gene sample 1 of the present invention is seated, the sample stand 2 and Supplying the heat medium M1 of the first temperature stored in the first heat medium storage 111 to the heat exchange block B in thermal contact (S11), and the second heat medium to the heat exchange block B. Supplying the thermal medium M2 of the second temperature stored in the reservoir 121 (S12) and the thermal medium M3 of the third temperature stored in the third thermal medium storage 131 in the heat exchange block B; Supplying step (S13) may be made.

Therefore, as shown in FIG. 13, the first temperature section A1 is a heating temperature for dismantling both male and female chains of the gene, and the second temperature section A2 is a cooling temperature for annealing both male and female chains of the disassembled gene. ) And the annealed male and female chains can achieve an ideal temperature range without overshooting or undershooting, respectively, in maintaining a third temperature section A3, which is a reheating temperature which replicates itself and its corresponding chain. .

On the other hand, as shown in Figure 3, the optical device 20 is a device for detecting an optical change according to the temperature of the gene sample 1, the light source 21, the first filter (F1), The light guide member 22, the beam splitter 23, the second filter F2, and the analyzer 23 may be included.

Here, the light source 21 may be any light source of various forms that can be provided by generating light such as LED, xenon lamp, halogen lamp.

In addition, the first filter F1 may be configured in the form of a wheel in which several filters are rotated so as to select only light having various wavelengths for the light generated by the light source 21.

In addition, the light guide member 22 may be a plastic fiber optic or an optical fiber that guides the light selected by the first filter F1 toward the genetic sample 1.

In addition, the beam splitter 23 transmits the light guided by the light guide member 22 to the gene sample 1, reflects the reaction light generated by the gene sample 1, and the light guide. It is a kind of optical mechanism for fixing the member 22.

In addition, the second filter F2 may be configured in the form of a wheel in which several filters are rotated to convert the reaction light into various desired shapes.

In addition, the analyzer 23 is an analyzer capable of confirming gene amplification by analyzing the reaction light converted by the second filter F2. The analyzer 23 may be applied to an analyzer capable of examining a specific fluorescent substance included in the gene sample 1.

On the other hand, as shown in Figures 3 to 8, the sample table transfer device 30, the gene sample (2) in the sample table 2 of Figure 6 is exposed to the outside of the case 102 of FIG. When the 1) is seated, as shown in FIG. 7, the apparatus for moving the sample stage 2 on which the gene sample 1 is seated in the direction of the heat exchange block B inside the case 102 is provided. In addition, a feeder using a variety of actuators such as a motor or a hydraulic cylinder as a driving source may be applied.

Subsequently, as shown in FIG. 8, after the heat exchange block B repeats a series of processes of heating and cooling the sample stage 2, the optical device 20 performs a plurality of gene samples ( 1) can be scanned sequentially.

It is needless to say that the present invention is not limited to the above-described embodiment, and can be modified by those skilled in the art without departing from the spirit of the present invention.

Therefore, the scope of the claims in the present invention will not be defined within the scope of the detailed description, but will be defined by the following claims and their technical spirit.

1: Gene sample 2: Sample stand
10: thermostat 20: optical device
30: sample to transfer device 100: gene amplification system
101: main body 102: case
B: heat exchange block 11: first heat medium supply
M1: thermal medium at first temperature 111: first thermal medium storage
S1: first temperature sensor 112: first temperature holding control unit
H1; Heater 113: first temperature holding device
L1: circulation line V1: first valve
P1: first pump 12: second thermal medium supply device
M2: heat medium at the second temperature 121: second heat medium reservoir
S2: second temperature sensor 122: second temperature holding control unit
H2; Heater 123: second temperature holding device
L2: circulation line V2: second valve
P2: second pump 13: third thermal medium supply
M3: thermal medium at third temperature 131: third thermal medium storage
S3: third temperature sensor 132: third temperature holding control unit
H3; Heater 133: third temperature holding device
L3: circulation line V3: third valve
P3: 3rd pump 14: control part
21: light source F1: first filter
22: light guide member F2: second filter
23: Analyzer IVP: Input Valve Package
OVP: output valve package VA: valve assembly
A1: first temperature section A2: second temperature section
A3: third temperature section 23: beam splitter

Claims (10)

A sample stage on which a gene sample is placed;
A temperature control device for heating and cooling the sample stage to adjust the temperature of the gene sample; And
It comprises a; optical device for detecting an optical change according to the temperature of the gene sample,
The temperature control device,
A heat exchange block in thermal contact with the sample stage;
A first heat medium supply device for supplying a heat medium of a first temperature to the heat exchange block;
A second heat medium supply device for supplying a heat medium of a second temperature to the heat exchange block;
A third heat medium supply device for supplying a heat medium of a third temperature to the heat exchange block; And
The first heat medium supplying device to sequentially supply the heat medium at the first temperature, the heat medium at the second temperature, and the heat medium at the third temperature to the heat exchange block according to a series of programs or commands; A control unit for applying a control signal to the second thermal medium supply device and the third thermal medium supply device;
Gene amplification system comprising a. The method of claim 1,
The first temperature is a heating temperature for dismantling both male and female chains of the gene, and the second temperature is a cooling temperature for annealing both male and female chains of the disassembled gene, and the third temperature is the female and female chains annealed. A gene amplification system, characterized in that the reheating temperature for replicating each of the chain corresponding to itself. The method of claim 1,
Wherein said first temperature is in the range of 90 degrees to 100 degrees Celsius, said second temperature is in the range of 50 degrees to 70 degrees Celsius, and said third temperature is in the range of 70 degrees to 80 degrees Celsius system. The method of claim 1,
The first thermal medium supply device,
A first thermal medium reservoir containing a thermal medium at a first temperature;
A first temperature holding device installed in the first thermal medium storage and maintaining the first thermal medium at a first temperature by using a first temperature sensor, a first temperature holding control unit and a heater;
A circulation line connecting the first heat medium reservoir to the heat exchange block and circulating the heat medium;
A first valve installed in the circulation line and regulating the circulation of the thermal medium at the first temperature; And
A first pump installed in the circulation line and circulating the heat medium having the first temperature;
Gene amplification system comprising a. The method of claim 1,
The optical device,
A light source for generating and providing light;
A first filter for converting light generated from the light source;
A light guide member for guiding light converted by the first filter toward a gene sample;
A beam splitter which transmits the light induced by the light guide member to a gene sample and reflects the reaction light generated from the gene sample;
A second filter for converting the reflected reaction light; And
An analyzer for analyzing the reaction light converted by the second filter;
Gene amplification system comprising a. In constructing a temperature control device of the gene amplification system for heating and cooling the sample stage to control the temperature of the gene sample,
A heat exchange block in thermal contact with the sample stage; And
And a first heat medium supply device for supplying a heat medium having a first temperature to the heat exchange block.
The first thermal medium supply device,
A first thermal medium reservoir containing a thermal medium at a first temperature;
A first temperature holding device installed in the first thermal medium storage and maintaining the thermal medium at a first temperature by using a first temperature sensor, a first temperature holding control unit and a heater;
A circulation line connecting the first heat medium reservoir and the heat exchange block;
A first valve installed in the circulation line and regulating the circulation of the thermal medium at the first temperature; And
A first pump installed in the circulation line and circulating the heat medium having the first temperature;
Temperature control device of the gene amplification system comprising a. The method according to claim 6,
And a second heat medium supply device for supplying a heat medium of a second temperature to the heat exchange block.
The second thermal medium supply device,
A second thermal medium reservoir containing a thermal medium at a second temperature;
A second temperature holding device installed in the second thermal medium storage and maintaining the thermal medium at a second temperature by using a second temperature sensor, a second temperature holding control unit and a heater;
A circulation line connecting the second heat medium reservoir to the heat exchange block;
A second valve installed in the circulation line and regulating the circulation of the thermal medium at the second temperature; And
A second pump installed in the circulation line and circulating the heat medium having the second temperature;
Temperature control device of the gene amplification system comprising a. 8. The method of claim 7,
And a third heat medium supply device for supplying a heat medium of a third temperature to the heat exchange block.
The third thermal medium supply device,
A third thermal medium reservoir containing a thermal medium at a third temperature;
A third temperature holding device installed in the third thermal medium storage and maintaining the thermal medium at a third temperature by using a third temperature sensor, a third temperature holding control unit and a heater;
A circulation line connecting the third heat medium reservoir to the heat exchange block;
A third valve installed in the circulation line and regulating the circulation of the thermal medium at the third temperature; And
A third pump installed in the circulation line and circulating the heat medium having the third temperature;
Temperature control device of the gene amplification system comprising a. The method of claim 8,
The first valve, the second valve and the third valve comprise a valve assembly comprising an input valve package having an input of 3 way and an output of 1 way and an output valve package having an input of 1 way and an output of 3 way. Temperature control device of the gene amplification system, characterized in that. In the temperature control method of the gene amplification system for heating and cooling the sample stage on which the gene sample is seated,
Supplying a heat medium of a first temperature stored in a first heat medium reservoir to a heat exchange block in thermal contact with the sample stage;
Supplying a heat medium of a second temperature stored in a second heat medium reservoir to the heat exchange block; And
Supplying a heat medium of a third temperature stored in a third heat medium reservoir to the heat exchange block;
Temperature control method of the gene amplification system comprising a.

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