KR20120071546A - Silk artificial membrane for artificial dural membrane and biological membrane with excellent flexibility and solidity - Google Patents
Silk artificial membrane for artificial dural membrane and biological membrane with excellent flexibility and solidity Download PDFInfo
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/04—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres having existing or potential cohesive properties, e.g. natural fibres, prestretched or fibrillated artificial fibres
- D04H1/08—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres having existing or potential cohesive properties, e.g. natural fibres, prestretched or fibrillated artificial fibres and hardened by felting; Felts or felted products
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- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
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- A61L2430/32—Materials or treatment for tissue regeneration for nerve reconstruction
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Abstract
Description
본 발명은 인공뇌경막 및 생체막용 실크 인공막에 관한 것으로, 특히 유연성과 봉합강도가 우수하여 시술 및 처치가 용이한 인공뇌경막 및 생체막용 실크 인공막에 관한 것이다.
The present invention relates to silk artificial membranes for artificial brain dural and biological membranes, and more particularly, to artificial membranes and artificial membranes for artificial membranes having excellent flexibility and susceptibility for easy treatment and treatment.
두개골의 내면을 덮고 있으며 대뇌 반구와 대뇌와 소뇌 사이, 뇌와 뇌하수체 사이 등에 돌출되어 있는 뇌경막은 뇌 수술시 불가피하게 손상된다.Covering the inner surface of the skull and projecting between the cerebral hemispheres and the cerebrum and cerebellum, between the brain and the pituitary gland inevitably damaged during brain surgery.
이 때문에, 손상된 뇌경막을 대체하는 인공뇌경막의 사용량이 증가하고 있는 추세인데, 세계적으로는 1조 규모의 시장이, 국내는 300억 규모의 시장이 형성되어 있다.For this reason, the amount of artificial dura mater replacing the damaged dura mater is increasing, but a market of 1 trillion in the world and a market of 30 billion in Korea are formed.
뇌경막용 재료 중 인체유래 뇌경막은 크로이츠펠트-야콥병의 감염 가능성이 크기 때문에 현재는 사용이 금지되어 있다.Among the materials for the dura mater, human-derived dura mater is currently prohibited due to the high possibility of infection with Creutzfeldt-Jakob disease.
이에, 최근에는 동물(소) 유래 콜라겐을 이용하여 제작한 인공뇌경막을 임상에서 사용하고 있다.In recent years, artificial brain dural membranes prepared using animal (cow) -derived collagen have been used in clinical practice.
한국 등록특허공보 제10-100461475호에서는 콜라겐 초미세 섬유성 부직포상 다층체를 비섬유화 콜라겐층의 사이에 둔 적층체로 이루어지는 콜라겐재를 뇌경막, 심막, 흉막, 복막, 또는 장막 등 생체막의 결손 부분을 보충하는 재료로서 사용할 수 있도록 제안하였다.In Korean Patent Publication No. 10-100461475, a collagen material composed of a laminate in which a collagen ultrafine fibrous nonwoven multilayer body is sandwiched between non-fibrous collagen layers is used to remove defects of biological membranes such as the brain dura, pericardium, pleural cavity, peritoneum, or intestinal membrane. It is proposed to be used as a supplementary material.
한국 공개특허공보 공개번호 제10-2001-0052714호에서는 콜라겐의 초미세섬유를 기본 단위로 하는 콜라겐 섬유의 부직포상 다원 구조체인 매트릭스 중에 생체 적합성을 가지며 또한 생체내에서 분해 흡수될 수 있는 물질을 충전하거나 개재시켜 이루어지는 콜라겐재를 인공 신경관, 인공 척수, 인공 식도, 인공 기관, 인공 혈관, 인공 밸브, 인공 뇌경막 등의 의료용 인공막, 인공 인대, 인공 건, 외과용 봉합사, 외과용 충전재, 외과용 보강재, 창상 보호재, 인공 피부 또는 인공 각막에 적용할 수 있도록 제안하였다.Korean Patent Laid-Open Publication No. 10-2001-0052714 fills a matrix that is biocompatible and capable of being decomposed and absorbed in vivo in a matrix, which is a nonwoven polymorphic structure of collagen fibers based on collagen ultrafine fibers. Or synthetic collagen material, including artificial neural tube, artificial spinal cord, artificial esophagus, artificial organ, artificial blood vessel, artificial valve, artificial dura mater, artificial ligament, artificial ligament, artificial gun, surgical suture, surgical filler, surgical reinforcement material, It is proposed to be applied to wound protectors, artificial skin or artificial cornea.
그러나, 동물(소) 유래 콜라겐을 이용하여 제작한 인공뇌경막은 광우병 등 인수 감염이 우려된다는 심각한 문제가 있다.However, the artificial brain dense produced using animal (cow) -derived collagen has a serious problem of susceptible infection such as mad cow disease.
이와 같은 중대한 결함에도 불구하고, 동물(소) 유래 콜라겐을 이용한 인공 뇌경막을 대체할 만한 대체품이 없는 것이 현실이며, 현재 주로 개발되고 있는 합성생체재료를 이용한 인공뇌경막 소재는 생체안전성과 봉합강도 등 기계적 강도 등의 문제로 아직 상용화되지 못하고 있는 상황이다.
Despite such serious defects, there is no substitute to replace artificial dura mater using animal-derived collagen. Currently, the artificial dural material using synthetic biomaterials, which are mainly developed, is mechanically secured such as biosafety and suture strength. It is not yet commercialized due to problems such as strength.
본 발명의 목적은 유연성과 봉합강도가 우수하여 시술 및 처치가 용이한 실크 인공뇌경막 및 생체막용 실크 인공막을 제공하는데 있다.
Disclosure of Invention An object of the present invention is to provide a silk artificial brain dura mater and a silk artificial membrane for biological membranes, which are excellent in flexibility and suture strength, and are easily treated and treated.
상기 목적을 달성하기 위한 본 발명의 실크 인공막은 실크 피브로인과 염을 포함한다.Silk artificial membrane of the present invention for achieving the above object includes silk fibroin and salt.
본 발명의 실크 인공막은 실크 피브로인 50.00?99.99중량%와 염 0.01?50.00중량%로 이루어짐이 좋다.Silk artificial membrane of the present invention is preferably made of 50.00 ~ 99.99% by weight of silk fibroin and 0.01 ~ 50.00% by weight of salt.
상기 염은 염화칼슘, 염화나트륨, 염화칼륨, 아세트산나트륨 중 어느 하나를 사용하는 것이 좋다.The salt is preferably used any one of calcium chloride, sodium chloride, potassium chloride, sodium acetate.
한편, 본 발명의 실크 인공막 제조방법은 실크 피브로인 50.00?99.99중량%에 염 0.01?50.00중량%를 혼합하여 혼합액을 만드는 단계 및 상기 혼합액을 평평한 용기에 붓고 건조하여 실크 인공막을 제조하는 단계를 포함한다.On the other hand, the method for producing a silk artificial film of the present invention comprises the step of mixing the silk fibroin 50.00 ~ 99.99% by weight of 0.01 to 50.00% by weight of salt to make a mixed solution and pouring the mixed solution in a flat container and dried to produce a silk artificial film .
상기 건조하는 단계에서, 상기 용기에 담긴 혼합액을 20~80℃, 20~80% 수분을 함유하는 항온실에서 건조하는 것이 좋다.
In the drying step, it is preferable to dry the mixed solution contained in the container in a constant temperature room containing 20 ~ 80 ℃, 20 ~ 80% moisture.
본 발명에 의해, 인공막의 안정성에 대한 우려를 불식하고, 유연성이 크게 보강되어 시술 및 처치가 용이하다.According to the present invention, concerns about the stability of the artificial membrane are eliminated, and the flexibility is greatly enhanced, so that the procedure and the treatment are easy.
또한, 300㎛이상의 두꺼운 막을 제작할 수 있기 때문에 두께 조절이 가능하다.In addition, since a thick film of 300 µm or more can be produced, the thickness can be adjusted.
또한, 유연성 뿐만 아니라 투명하며 봉합강도가 우수하기 때문에 시술이 더 용이하다.In addition, the procedure is easier because it is not only flexible but also transparent and has excellent suture strength.
또한, 세포독성이 없으며, 척수의 보호 및 뇌 척수액의 누출을 방지할 수 있는 방수성을 갖는다.
In addition, there is no cytotoxicity, and it is waterproof to protect the spinal cord and prevent leakage of cerebrospinal fluid.
도 1은 본 발명의 실크 인공막의 일예인 실크 인공뇌경막의 신장율을 나타낸 그래프이다.
도 2는 본 발명의 실크 인공막의 일예인 실크 인공뇌경막의 단면 및 표면 사진도면이다.
도 3a는 본 발명의 실크 인공막의 일예인 실크 인공뇌경막의 투명도를 나타낸 사진도면이다.
도 3b는 기존의 소유래 콜라겐으로 만든 인공뇌경막의 투명도를 나타낸 도면이다.
도 4는 본 발명의 실크 인공막의 일예인 실크 인공뇌경막의 유연성을 나타낸 사진도면이다.
도 5는 본 발명의 실크 인공막의 일예인 실크 인공뇌경막의 봉합강도를 나타낸 사진도면이다.
도 6a는 24시간 배양시 본 발명의 실크 인공막의 일예인 실크 인공뇌경막의 세포독성 결과를 나타낸 그래프이다.
도 6b는 48시간 배양시 본 발명의 실크 인공막의 일예인 실크 인공뇌경막의 세포독성 결과를 나타낸 그래프이다.
도 6c는 72시간 배양시 본 발명의 실크 인공막의 일예인 실크 인공뇌경막의 세포독성 결과를 나타낸 그래프이다.1 is a graph showing the elongation rate of the silk artificial brain dura as an example of the silk artificial membrane of the present invention.
2 is a cross-sectional and surface photographic view of the silk artificial brain dura as an example of the silk artificial membrane of the present invention.
Figure 3a is a photographic view showing the transparency of the silk artificial brain dura as an example of the silk artificial membrane of the present invention.
Figure 3b is a view showing the transparency of the artificial dura mater made from conventional proprietary collagen.
Figure 4 is a photographic view showing the flexibility of the silk artificial brain dura as an example of the silk artificial membrane of the present invention.
5 is a photographic view showing the suture strength of the silk artificial brain dura as an example of the silk artificial membrane of the present invention.
Figure 6a is a graph showing the cytotoxicity results of silk artificial brain dura, an example of the silk artificial membrane of the present invention in 24 hours culture.
Figure 6b is a graph showing the cytotoxicity results of silk artificial brain dura, an example of the silk artificial membrane of the present invention at 48 hours culture.
Figure 6c is a graph showing the cytotoxicity results of silk artificial brain dura, an example of the silk artificial membrane of the present invention in 72 hours culture.
이하, 본 발명의 바람직한 실시예를 설명하면 다음과 같다.Hereinafter, preferred embodiments of the present invention will be described.
본 발명의 실크 인공막은 실크 피브로인과 염으로 이루어진다.The silk artificial membrane of this invention consists of silk fibroin and a salt.
실크 피브로인은 세리신과 함께 고치실(실크)을 구성하는 단백질이다.Silk fibroin is a protein that, together with sericin, forms a cocoon (silk).
실크 피브로인은 18종의 아미노산이 비교적 골고루 존재하며, 양모섬유의 케라틴 단백질과는 달리 글리신과 알라닌의 함량이 매우 높다.Silk fibroin is relatively evenly present in 18 amino acids. Unlike the keratin protein of wool fibers, silk fibroin has a high content of glycine and alanine.
인체 피부를 구성하고 있는 주요 단백질인 콜라겐 단백질의 아미노산 중에서 가장 많은 성분이 피브로인 단백질 중의 글리신과 알라닌이다.Among the amino acids of the collagen protein, the main protein constituting the human skin, the most components are glycine and alanine in the fibroin protein.
이처럼 사람의 피부와 유사한 아미노산 구조를 갖는 피브로인 단백질은 생체적합성이 우수하고 염증 반응이 없는 효과를 나타낸다.Fibroin protein having an amino acid structure similar to that of human skin has excellent biocompatibility and no inflammatory response.
이 때문에, 실크 피브로인을 주성분으로 하는 본 발명의 실크 인공막은 안정성에 대한 우려를 불식시킬 수 있는 것이다.For this reason, the silk artificial film of this invention which has silk fibroin as a main component can dispel the concern about stability.
본 발명의 실크 인공막을 이루는 다른 주성분인 염은 실크 피브로인만으로 두꺼운 막을 만들 경우에 발생되는 딱딱하게(stiff)되는 증상을 유연하게 (flexible) 만들어 줌으로써, 본 발명의 실크 인공막이 유연성을 갖도록 한다.Salt, which is another main component of the silk artificial film of the present invention, makes the silk artificial film of the present invention flexible by making flexible the stiff symptoms occurring when a thick film is made only with silk fibroin.
또한, 염은 본 발명의 실크 인공막의 강도를 높여준다.In addition, the salt increases the strength of the silk artificial membrane of the present invention.
또한, 상기 염은 어떠한 염계열의 물질을 사용하여도 무방하나, 염화칼슘, 염화나트륨, 염화칼륨, 아세트산나트륨 중 어느 하나를 사용하는 것이 바람직하다.In addition, the salt may be any salt-based material, it is preferable to use any one of calcium chloride, sodium chloride, potassium chloride, sodium acetate.
결국, 본 발명의 실크 인공막은 유연성과 강도가 우수하기 때문에 시술 및 처치가 아주 용이한데, 특히 봉합강도가 매우 우수하기 때문에 척수의 보호 및 뇌 척수액의 누출을 방지할 수 있는 방수성을 더 갖으므로, 실크 인공뇌경막으로 사용하는데 매우 적합하다.After all, the silk artificial membrane of the present invention is very easy to perform the procedure and treatment because of its excellent flexibility and strength, in particular, because it has a very good suture strength and further has a waterproof property to prevent the leakage of cerebrospinal fluid and protection of the spinal cord, It is very suitable for use as a silk artificial brain dura mater.
게다가, 본 발명의 실크 인공막은 투명성을 갖기 때문에 수술시 안전한 처치를 가능토록 하고, 사용이 안전하기 때문에 세포독성도 없어 생체막으로 광범위하게 사용하는데 문제가 없다.In addition, since the silk artificial membrane of the present invention has transparency, it enables safe treatment during surgery, and because it is safe to use, there is no cytotoxicity and thus there is no problem in using it as a biofilm widely.
또한, 본 발명의 실크 인공막은 300㎛이상의 두께를 갖는 것이 가능하기 때문에 두께 조절이 가능하다.In addition, since the silk artificial film of the present invention can have a thickness of 300 μm or more, the thickness can be adjusted.
이와 같은 본 발명의 실크 인공막은 50.00?99.99중량%의 실크 피브로인과, 0.01?50.00중량%의 염으로 이루어짐이 바람직하다.Such a silk artificial film of the present invention is preferably composed of 50.00 ~ 99.99% by weight of silk fibroin, and 0.01 ~ 50.00% by weight of salt.
실크 피브로인이 50.00중량% 미만이면, 막을 형성하는데 문제가 있고, 99.99중량%를 초과하면, 막의 유연성이 저하되기 때문에, 본 발명의 실크 인공막은 50.00?99.99중량%의 실크 피브로인을 함유함이 좋다.If the silk fibroin is less than 50.00% by weight, there is a problem in forming the film, and if the silk fibroin is more than 99.99% by weight, the flexibility of the film is lowered.
또한, 염이 0.01중량% 미만이면, 막의 유연성이 저하되는 문제가 있고, 50.00중량%를 초과하면, 실크 피브로인이 막을 형성하는데 문제가 있기 때문에, 본 발명의 실크 인공막은 0.01?50.00중량%의 염을 함유함이 좋다.In addition, if the salt is less than 0.01% by weight, there is a problem that the flexibility of the film is lowered, and if the salt is more than 50.00% by weight, the silk fibroin has a problem in forming a film, the silk artificial membrane of the present invention is 0.01-50.00% by weight of salt It is good to contain.
이와 같은 본 발명의 실크 인공막은 액상의 실크 피브로인 50.00?99.99중량%에 액상의 염 0.01?50.00중량%를 혼합하여 혼합액을 만들고, 그 혼합액을 평평한 용기에 부어 건조시킴으로써 제조된다.
Such a silk artificial film of the present invention is prepared by mixing 0.01-50.00% by weight of a liquid salt with 50.00-99.99% by weight of liquid silk fibroin to make a mixed solution, and pouring the mixed solution into a flat container and drying it.
이하, 실시예 및 실험예는 오로지 본 발명의 일예를 설명하는 것으로, 이 실시예 및 실험예에 의해 본 발명의 범위가 제한되지 않음을 밝혀둔다.The following Examples and Experimental Examples are only illustrative of the present invention, and the scope of the present invention is not limited by the Examples and Experimental Examples.
＜실시예 1＞ 실크 인공뇌경막 제조<Example 1> Silk artificial brain dura
시료로 누에고치를 사용하였으며 세리신을 제거하기 위하여 마리세유 비누로 100℃에서 정련하였다.A silkworm cocoon was used as a sample and refined at 100 ° C. with Marise oil soap to remove sericin.
정련된 실크를 염화칼슘, 에탄올, 물 1:2:8 (몰비율)로 제작한 염화칼슘-알콜-수용액으로 80℃에서 20분 동안 용해하였다.The refined silk was dissolved in calcium chloride, ethanol, and calcium chloride-alcohol-aqueous solution prepared in water 1: 2: 8 (molar ratio) at 80 ° C. for 20 minutes.
이 실크 수용액을 투석막 (MWCO=12,000)에 넣고 3일 동안 증류수에 투석하여 염과 에탄올을 제거하였다.This aqueous silk solution was placed in a dialysis membrane (MWCO = 12,000) and dialyzed in distilled water for 3 days to remove salt and ethanol.
만들어진 실크 피브로인 수용액의 최종 농도는 약 2.5% 정도였으며 이를 7~8%의 농도가 되도록 PEG 20,000(polyethylene glycol)을 이용하여 농축하였다.The final concentration of the silk fibroin solution produced was about 2.5% and concentrated using PEG 20,000 (polyethylene glycol) to a concentration of 7 ~ 8%.
농축된 실크 피브로인 용액 50.00?99.99중량%에 염화칼슘 용액 0.01?50.00중량%를 가한 후 교반기를 이용하여 잘 혼합하고 이 혼합액을 평평한 용기에 부은 후 25℃, 50% 수분을 함유하는 항온실에서 건조하여 본 발명의 일예인 실크 인공 뇌경막을 제조하였다.
Add 0.01 to 50.00% by weight of calcium chloride solution to 50.00 ~ 99.99% by weight of the concentrated silk fibroin solution, mix well using a stirrer, pour the mixture into a flat container, and dry in a constant temperature room containing 25 ° C and 50% moisture. An artificial silk dura was prepared as an example of the present invention.
＜실험예 1＞ 실크 인공뇌경막의 구조적 특성 및 물성실험Experimental Example 1 Structural Characteristics and Physical Properties of Silk Artificial Brain Dural
실시예1에서 제작한 본 발명의 일예인 인공뇌경막의 두께를 두께 측정기를 이용하여 측정하였으며, FT-IR 분석으로 구조를 관찰하였다.The thickness of the artificial dura, which is an example of the present invention produced in Example 1, was measured using a thickness gauge, and the structure was observed by FT-IR analysis.
주사전자 현미경(SEM)을 이용하여 표면과 단면 구조를 관찰하였다.Surface and cross-sectional structures were observed using a scanning electron microscope (SEM).
인장강도 시험기를 이용하여 실시예1에서 제작한 본 발명의 일예인 실크 인공뇌경막의 인장강도와 절단 신장률을 측정하였다.
Tensile strength and elongation at break of the silk artificial brain dura, which is an example of the present invention prepared in Example 1, were measured using a tensile strength tester.
＜실험예 2＞세포배양 및 세포독성 실험Experimental Example 2 Cell Culture and Cytotoxicity Experiment
세포 독성 시험을 위하여, L929 섬유아세포를 ATCC로부터 구입하여 10% 소혈청, 50 ㎍/ml 스트렙토마이신과 100 U/ml 페니실린이 함유된 MEM 배지에서 각 well 당 10,000개의 세포를 접종후 24시간동안 배양하였다.For cytotoxicity testing, L929 fibroblasts were purchased from ATCC and incubated for 24 hours after inoculation with 10,000 cells per well in MEM medium containing 10% bovine serum, 50 μg / ml streptomycin and 100 U / ml penicillin. It was.
실시예1에서 제작한 본 발명의 일예인 실크 인공뇌경막을 1×1cm로 자르고 뇌경막 시료 1g에 배지 10ml을 가하여 24시간 동안 37℃ CO2 배양기 내에서 용출하였다.The silk artificial brain dura, an example of the present invention prepared in Example 1, was cut into 1 × 1 cm, and 10 ml of medium was added to 1 g of the dural sample, and eluted in a 37 ° C. CO 2 incubator for 24 hours.
용출된 시료를 배지를 이용하여 연속해서 희석한 후 미리 배양한 세포에 처리하였으며, MTT 방법을 이용하여 세포 생존률을 평가하였다.
The eluted samples were serially diluted with media and then treated with pre-cultured cells, and cell viability was evaluated using the MTT method.
＜결과 1＞ 실크 인공뇌경막의 제조 결과<Result 1> Production result of silk artificial brain dura
실크 피브로인 만으로 제작되는 실크 인공뇌경막의 경우 투명성이 있는 강점이 있으나 두께가 300㎛ 이하였다. 그러나, 실시예1에서 제작된 본 발명의 일예인 실크 인공뇌경막은 두께가 300㎛ 이상, 나아가 500㎛이상 까지도 조정이 가능하다.Silk brain dura mat made of only silk fibroin has transparency but strong thickness 300 micrometers or less. However, the silk artificial brain dura, which is an example of the present invention produced in Example 1, can be adjusted to a thickness of 300 μm or more, and even 500 μm or more.
실크 피브로인을 가지고 인공뇌경막을 제조하는 기존의 제조법으로 실크 인공뇌경막을 제작할 경우, 실크 인공뇌경막이 300㎛ 이상의 두께를 가지면 강점이었던 투명성 마저 사라져 버리나, 본 발명은 투명성을 유지하면서도 두께가 두꺼운 실크 인공뇌경막을 제작할 수 있다.
In the case of producing a silk artificial brain dura using a conventional manufacturing method of the artificial brain dural with silk fibroin, when the silk artificial brain dura has a thickness of 300 μm or more, transparency, which is a strength, disappears, but the present invention maintains transparency and thick silk artificial brain dura Can be produced.
＜결과 2＞ 실크 인공뇌경막의 구조적 특성 및 물성실험 결과<Result 2> Structural Characteristics and Physical Properties of Silk Artificial Brain Dural
실시예1에서 제작된 본 발명의 일예인 실크 인공뇌경막은 표 1과 도 1의 그래프도에서 알 수 있는 바와 같이 유연성과 탄성 및 봉합강도를 가지며, 도 2의 단면 및 표면 사진도면(위 사진도면은 실크만 사용해서 만든 인공뇌경막의 단면 및 표면, 아래 사진도면은 본 발명의 단면 및 표면)에서 알 수 있는 바와 같이 300㎛이상의 두께를 가지면서도 매끈하고 일정한 표면과 단면을 가진다. Silk artificial brain dural, which is an example of the present invention produced in Example 1 has flexibility, elasticity and suture strength as shown in Table 1 and the graph of Fig. 1, the cross-sectional and surface photographic drawing of Figure 2 (pictured above) The cross section and the surface of the artificial brain film made using only silver silk, as shown in the cross-sectional view and the surface of the present invention below) has a smooth and uniform surface and cross-section while having a thickness of more than 300㎛.
본 발명의 일예인 실크 인공뇌경막의 경우 356.7%까지 신장되어 신장강도가 크게 개선되었다.In the case of the silk artificial brain dura, an example of the present invention, the elongation was greatly improved by 356.7%.
또한 도 3a 및 도 3b에서 알 수 있는 바와 같이, 본 발명의 일예인 실크 인공뇌경막은 투명성을 갖춤으로써 뇌신경외과 수술시 안전한 처치를 가능케 한다.In addition, as can be seen in Figures 3a and 3b, the silk artificial brain dura, an example of the present invention has transparency to enable safe treatment during cerebral neurosurgery surgery.
또한 도 4에서 알 수 있는 바와 같이, 본 발명의 일예인 실크 인공뇌경막은 상당한 유연성을 갖으며, 도 5에서 알 수 있는 바와 같이 수술시 적합한 봉합강도를 유지한다.
In addition, as can be seen in Figure 4, the silk artificial brain dura, an example of the present invention has considerable flexibility, and as shown in Figure 5 maintains a suitable suture strength during surgery.
＜결과 3＞ 세포독성실험 결과<Result 3> Results of Cytotoxicity Test
실시예1에서 제작된 본 발명의 실크 인공뇌막에 함유된 염화 칼슘 및 실크의 세포 독성실험을 실시한 결과, 도 6a?도 6b에서와 같이 실크 인공뇌경막 추출물의 농도에 상관없이 24, 48, 72시간 동안 거의 100%의 생존율을 보였다. As a result of the cytotoxicity test of calcium chloride and silk contained in the silk prosthesis of the present invention prepared in Example 1, as shown in Figs. 6a to 6b, irrespective of the concentration of the silk prosthetic meninges extract, 24, 48 and 72 hours Almost 100% survival rate.
이 결과는 본 발명의 일예인 실크 인공뇌경막에 함유된 실크 뿐아니라 염화 칼슘도 세포독성을 나타내지 않음을 가리킨다.This result indicates that calcium chloride as well as silk contained in the silk artificial brain dura, which is an example of the present invention, do not exhibit cytotoxicity.
Claims (7)
A silk artificial film comprising silk fibroin and salts.
상기 실크 피브로인 50.00?99.99중량%와 상기 염 0.01?50.00중량%로 이루어진, 실크 인공막.
The method of claim 1,
A silk artificial film comprising 50.00 to 99.99% by weight of the silk fibroin and 0.01 to 50.00% by weight of the salt.
상기 염은 염화칼슘, 염화나트륨, 염화칼륨, 아세트산나트륨 중 어느 하나인 것이 특징인, 실크 인공막.
The method of claim 1,
The salt is a silk artificial film, characterized in that any one of calcium chloride, sodium chloride, potassium chloride, sodium acetate.
실크 인공막.
Using the silk artificial membrane of any one of claims 1 to 3 as an artificial brain dural or biological membrane,
Silk artificial film.
상기 혼합액을 평평한 용기에 붓고 건조하여 실크 인공막을 제조하는 단계;를 포함하는, 인공뇌경막 및 생체막용 실크 인공막의 제조방법.
50.00 to 99.99% by weight of silk fibroin is mixed with 0.01 to 50.00% by weight of a salt to make a mixed solution; And
Pouring the mixed solution in a flat container and dried to produce a silk artificial film; comprising, a method for producing a silk artificial film for artificial brain dural and biological membranes.
상기 염은 염화칼슘, 염화나트륨, 염화칼륨, 아세트산나트륨 중 어느 하나인 것이 특징인, 인공뇌경막 및 생체막용 실크 인공막의 제조방법.
The method of claim 5,
The salt is characterized in that any one of calcium chloride, sodium chloride, potassium chloride, sodium acetate, method of producing a silk artificial membrane for artificial brain dural and biological membranes.
상기 건조하는 단계에서, 상기 용기에 담긴 혼합액을 20~80℃, 20~80% 수분을 함유하는 항온실에서 건조하는, 인공뇌경막 및 생체막용 실크 인공막의 제조방법.The method of claim 5,
In the drying step, the mixed solution contained in the container is dried in a constant temperature room containing 20 ~ 80 ℃, 20 ~ 80% moisture, artificial membranes and artificial membranes for the production of silk artificial membranes.
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