KR20120058658A - Face mask or filter material comprising Houttuynia cordata extract - Google Patents

Abstract

PURPOSE: A virus infection prevention mask and a filter are provided to prevent cause of diseases by suppressing the infection of virus from contaminated air. CONSTITUTION: A virus infection prevention mask comprises a retaining unit and a filter material. The retaining unit maintains a mask on the face of a user. The filter material passes the inhaled or exhaled air of a user. On the air permeable material, alcoholic extract of houttuynia cordata combined with acidic polymer is loaded. The alcoholic extract of houttuynia cordata is selected from a group consisting of water, lower alcohol, and mixed solvent thereof.

Description

Face mask or filter material comprising Houttuynia cordata extract}

The present invention relates to a mask or a novel filter capable of preventing disease by inhibiting the infectivity of the virus from inhaled air contaminated with influenza virus or contaminated air extracted from a patient infected with the virus. It relates to a mask or filter comprising and a manufacturing method thereof.

Human infectious diseases include various diseases such as respiratory diseases, which are caused by bacteria, fungi, and viruses. Viruses in particular have a size of less than 100nm, so it is not easy to filter them completely. Influenza virus infections occur every year, with intermittent pandemics that kill millions of people. In 2009, swine flu occurred, killing tens of thousands of people worldwide. Influenza viruses also infect animals, and birds and pigs also act as intermediate hosts for influenza viruses, and birds, in particular, contain the genotypes of all types of influenza viruses, playing a major role in the development of new influenza virus strains. It is thought. Inhalation of air containing the influenza virus can be a route of infection, particularly in infected patients or in people working with animals. The air from the breathing, coughing and sputum of an infected patient contains a myriad of viruses, and the feces of animals are also released, so if you work together or live in such an environment, you are more likely to be infected with the influenza virus. .

Masks incorporating the appropriate filter material are effective means of preventing the infection of viruses spreading into the air. Nonwoven fabrics are made of felt in the form of fibers that are arranged in parallel or in a non-directional direction and bonded with synthetic resin adhesives without going through a woven fabric process. Sombiscosrayon was primarily used as a raw material fiber, but from the mid-1950s Synthetic fibers such as nylon were also used. The processing method of nonwoven fabric is immersion type and dry type. Immersion is also called papermaking (抄紙 式), and the fibers are put into a synthetic resin glue bucket and dried and heat treated, similar to paper. Drying is the drying of fibers made of thin cotton with synthetic resins.

Recently, nonwoven fabrics have been used in masks as filter materials. Air filters for removing viruses or other microorganisms using such nonwoven materials are known. One type of such filter comprises a fibrous or particulate substrate, on which the substance which neutralizes the virus and / or other microorganisms in question on the substrate, ie on the surface of such fibers or particles, and / or in bulk thereof. Is calm. Examples of descriptions for such filters are as follows. US 3,871,950 and US 4,181,694 describe hollow fibers of ultrafiltration acrylonitrile polymers primarily for filtering aqueous media. U.S. Patent No. 4,856,509 describes a mask in which a portion of the mask contains a virus disrupting agent such as citric acid. U. S. Patent No. 5,767, 167 describes an aerogel foam suitable for filtering media for capturing microorganisms such as viruses and the like. U. S. Patent No. 5,783, 502 describes fibrous substrates having cationic groups, such as quaternary ammonium cationic hydrocarbon groups, bound to fibers, in particular antiviral molecules. U.S. Patent 5,851,395 describes a virus filter comprising a filter material, on which a virus trapping material based on sialic acid (9-carbon monosaccharide with carboxylic acid substituents on the ring) is deposited. . U. S. Patent No. 6,182, 659 describes a virus removal filter based on Streptococcus agalactiae culture. US 6,190,437 describes air filters for removing viruses from air, including carrier substrates deposited with “iodine resins”. U. S. Patent No. 6,379, 794 describes filters based on glass and other high modulus fibers deposited with acrylic latex. US Pat. No. 6,551,608 describes porous thermoplastic substrates and antiviral materials prepared by sintering one or more antiviral agents into a thermoplastic. U.S. Pat.No. 7,029,516 describes a filter system for removing particles from a fluid comprising a non-woven polypropylene substrate, on which an acidic polymer such as polyacrylic acid is deposited. US 2004/0250683 describes a filter material comprising a fiber of network structure with an acidic material, which may be an acrylic polymer, deposited thereon. US 2005/0247608 describes filter blocks that can be treated with various antiviral polymers, mainly cationic polymers. International Publication No. 2001/07090 describes a filter for removing microorganisms, including a substrate having a reactive surface and a polymer and comprising cationic groups for attracting the microorganisms on the surface. International Publication No. 2002/058812 describes air filters with micro-ncapsulated biologics. International Publication No. 2003/039713 discloses a filter material which is said to have an antipathogenic effect, including an effect on viruses, based on a fibrous substrate partially coated with a polymer network containing pendant functional groups which may be acidic groups. This is described. International Publication No. 2005/070242 describes a suction filter made of fibers that have been treated to impart a charge to trap particles such as viruses. British Patent No. 2035133 describes a membrane filter having a water insoluble polymer, preferably PVA, on its surface. Japanese Patent No. 2001/162116 describes an antibacterial filtration medium in which a self-crosslinked acrylic resin is used to bind a silver-organic idine antibacterial agent with a fibrous substrate. Japanese Patent No. 2005/198676 describes the use of a water-hardenable resin emulsion that binds citric acid to an antiviral mask. In the three papers of the Journal of Virology: Sept. 1968, p878-885; March 1970, p 313-320 and p 321-328, polyacrylic acid, polymethacrylic acid, and polyacetal carboxylic acid are described. The antiviral activity of the various polycarboxylic acids, including them, is described. The reported antiviral activity appears to be a cell-mediated effect, and the conclusions describe that PMAA (polymethacrylic acid) did not inactivate viral particles in the extracellular state.

It is already known to inhibit the proliferation of the virus when administered Echo extract. Eoseongcho extract describes that it has antiviral activity against coronavirus in Korean Patent No. 10-0637018. When Echo extract is administered to Vero cell culture with the corona virus, the growth of corona virus is suppressed. In the herbal medicine dictionary, it is reported that Eochochocho extract inhibits the growth of influenza virus. Korean Patent Application Nos. 2005-0089783 and 2005-0015031 also describe that Echo-chocho extract has antiviral activity against Evian influenza virus or Influenza virus. It is described that the growth of influenza virus is inhibited.

However, the conventional technique has only been reported to inhibit the proliferation of the virus in the cell by directly administering Echo extract to the culture medium of cells infected with the influenza virus, the effect on the outside of the cell has not been identified.

Against this background, the present inventors have made diligent efforts to develop masks and filter materials that can be prevented from virus contaminated air. The present invention was completed by confirming the utility as a personal protective device or a mask for preventing infection of influenza virus.

One object of the present invention is to provide an air permeable mask and a method of manufacturing the same, including Echo extract.

Still another object of the present invention is to provide an antiviral filter and a method of preparing the same, including the extract of Echochocho.

As an aspect to achieve the above object, the present invention is an air permeable mask of a shape suitable for close contact with the patient's face and placed on the user's mouth and nose, the mask is held in place on the user's face Means comprising at least one layer of filter material defined such that the intake and / or withdrawal air of the user passes through the filter material, wherein the filter material comprises an air permeable substrate loaded with a vinegar extract extracted with an acidic polymer; It relates to an air permeable mask.

In the present invention, the term " Houtuynia cordate " refers to the ground portion of the weak wheat of more than three hundred . Echoseong is an excellent antipyretic and cough due to lung abscess, pus, vomiting, pneumonia, acute bronchitis, enteritis, urinary tract infections, boils, fever and urine can also be used. The pharmacological action of Eoseongcho has been reported to be antibacterial, immune enhancing, anti-inflammatory, diuretic, and antitussive. Appearance has cell lines on stems and nodes are clear. The lower node has a thin root and the vagina is soft and easy to bend. The leaves are dried or squashed, but when they are spread out, they are heart shaped. The leaves are pointed at the end, the upper side is dark yellowish green or yellowish brown, and the lower side is grayish green or grayish brown. Echochocho extract according to the present invention can be prepared using any extraction method known in the art without limitation. Method for obtaining Eochocho extract in the present invention is as follows. About 2 to 20 times that of the Houttuynia cordata pulverized by dry weight, and preferably having a carbon number of ₁ (C 1) to ₄ (C 4) a polar solvent of a lower alcohol or those such as the volume of methanol, ethanol and butanol, up to about 10 times A mixed solvent having a mixing ratio of about 1: 0.1 to 1:10 was used as the elution solvent, and the extraction temperature was 20 to 100 ° C., preferably at room temperature, such as hot water extraction, cold extraction, reflux cooling extraction, or ultrasonic extraction. Extract using extraction method. Preferably, in the present invention, methanol and ethanol extracts of fish vinegar are prepared, and the antiviral activity of the mask using the filter immersed in the extract is measured. When the filter containing the fish vinegar extract as an active ingredient is applied to the mask, the virus It was confirmed that the removal activity was excellent (Table 1, Table 2 and Figure 2).

In the present invention, the term "mask" refers to an object covering the mouth and nose to prevent foreign substances such as germs and dust from penetrating into the body. More specifically, the mask of the present invention is an air-permeable mask that is placed on the user's mouth and nose and is suitable for close contact with the patient's face, provided with means for keeping the mask in place on the user's face and The inlet and / or aspirate air may comprise one or more layers of filter material defined to pass through the filter material, and the filter material may be an air permeable mask comprising an air permeable substrate associated with the fish leaf extract.

The overall shape of the face mask may generally be customary in the field of face masks, and the means by which the mask is held in place on the user's face is applicable to a variety of techniques known in the art, preferably the user It may be to include one or more elastic straps that fall behind the head of the.

The air permeable substrate of the mask of the present invention is applicable to various substrates known in the art, but is not limited thereto. Preferably it may be an air permeable substrate comprising a fibrous substrate, may be a woven or nonwoven fabric, more preferably a nonwoven fabric.

Examples of fabrics include natural and synthetic fibers such as cotton, cellulose, wool, polyolefins, polyesters, polyamides (such as nylon), rayon, polyacrylonitrile, cellulose acetate, polystyrene, polyvinyl, and fibers Any other synthetic polymer that can be processed into can be included, and examples of nonwoven fabrics can include polypropylene, polyethylene, polyester, nylon, PET, and PLA. Such materials may be in the form of nonwoven sheets or pads. The grade of fibrous substrate that can be used can be determined by the embodiment to properly vent the air, and the density can be determined as known from the mask art to provide a mask of comfortable weight.

 Typical nonwoven materials that have been shown to be suitable for use in the present invention have a weight of 10 to 40 g / m, but are not limited thereto, and other suitable materials known in the art can be determined to a suitable weight by one skilled in the art. The material at the top of this range can be rather heavy for use in masks.

As used herein, the term "virus" refers to a small particle that is smaller than a bacterium that is composed of a nucleic acid and a small number of proteins necessary for survival, and which lives on a host, and cannot be separated by a bacterial filter. Viruses are controversial between living and nonliving organisms because they can be obtained as crystals, but they are generally considered to be living beings because they have specific characteristics such as proliferation and heredity. Viruses can be divided into plant viruses and animal viruses, and plant viruses include tobacco mosaic virus, cassava mosaic virus, potato Y virus, sugar beet yellow virus, pea mosaic virus, cucumber mosaic virus, and the like. Examples include Japanese encephalitis virus, dengue virus, yellow fever virus, polio virus, coxsackie virus, echo virus, hepatitis virus, rabies virus, influenza virus, mumps virus, adenovirus and the like, preferably influenza virus, human parainfluenza Virus, human respiratory synstium virus, human metapneumovirus, human coronavirus, human rhinovirus or adenovirus, respiratory infection virus, most preferably the virus of the present invention is influenza Now it can be a virus. According to one embodiment of the present invention, it was confirmed that the influenza virus activity is significantly reduced when passing through the mask of the present invention.

As used herein, the term "antivirus" refers to the action of inhibiting, preventing or killing the growth of a virus. Masks loaded with Echo extract of the present invention was confirmed to have excellent activity in removing the human respiratory infection virus.

The air permeable mask of the present invention may be a mask comprising a fabric loaded with the Echochocho alcohol extract of the present invention. The Echochocho alcohol extract may be a mask for preventing human respiratory virus infection, characterized in that extracted with a solvent selected from the group consisting of water, lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. The loading amount of the Echochocho alcohol extract may be easily selected by those skilled in the art in an effective concentration range exhibiting antiviral activity, preferably 1-100 g / m ² . According to a specific embodiment of the present invention, the mask using the filter of the nonwoven fabric dried at 60 ℃ for 16 hours after immersion in the alcoholic extract of Eoseongcho of the present invention was confirmed that the influenza virus removal ability is excellent (Table 1 , And Table 2). The air permeable mask of the present invention can be used by a person skilled in the art to appropriately select a fabric composed of various types of weaving methods known in the art, preferably an air permeable mask composed of a single or a plurality of fabrics loaded with Echo extract Can be.

As another aspect, the present invention relates to a method for producing a mask, characterized in that the above-mentioned Echochocho extract having an antiviral function as an active ingredient.

As used herein, the term "anti-virus" is as described above, and preferably may be a function of inhibiting, preventing or killing the activity of the human respiratory syndrome virus.

The mask having an antiviral function of the present invention may be applied by appropriately changing or modifying a method of preparing a mask known in the art according to the use of the mask by the choice of a person skilled in the art. It can be an air permeable mask with antiviral function, including fabric loaded at 100 g / m ² .

In addition, the masks of the present invention can be made using mask processes known in the art, for example, molding / folding. A method of making a face mask is provided, including providing the filter material described in the present invention and forming the filter material into a face mask.

The face mask of the present invention may comprise one, two, three or more layers of sheet or pad-like filter material. The filter material of the present invention in the form of a sheet can be easily configured into a convex shape suitable for fitting to the face of a user. The face mask may further comprise one or more layers of additional material, such as, for example, one layer supporting the filter material, or two layers sandwiching the filter material, and optionally one or more additional layers. This additional layer of material, when used in the mask, may be placed in the face mask to reduce any irritation to the user's skin by placing the layer of additional material between the filter material and the user's skin.

Such additional materials may be woven or nonwoven, as described above. The nonwoven can be in the form of a nonwoven sheet or pad. Suitable grades of nonwoven polypropylene include well known grades commonly used in surgical face masks and the like. In addition, the substrate may also be in the form of an open-cell foam, such as a polyurethane foam, such as used for an air filter, such as in a nose air plug. Suitable materials for this additional material may be polyester, cellulose or nonwoven polypropylenes of the type commonly used in surgical masks and the like.

For example, the facial mask of the present invention may comprise a filter material comprising a vinegar extract deposited thereon, and an additional layer of nonwoven fabric defined between the filter material and the user's skin. The layer of filter material and the layer of further material can be loaded together into the fabric, for example around each edge, for example by ultrasonic welding. In one embodiment of the present invention, the loading solutions listed above were sprayed or immersed using a commercially available standard machine, and then the Echochocho alcohol extract was loaded onto the fabric by drying the wetted fibers in a drying tunnel.

In yet another aspect, the present invention relates to an antiviral filter comprising an air permeable substrate loaded with the above-mentioned fishery herb extract.

In the present invention, the term "filter" refers to effectively blocking various pollutants through the respiratory system to protect the worker and to effectively protect the respiratory organ from serious pollution in life.

The material usable in the filter of the present invention may be used without limitation as long as it is a material for preparing a filter known in the art, and preferably, may be a nonwoven fabric including a fibrous air permeable substrate. In addition, the fabric constituting the filter may be an air permeable filter composed of a single or a plurality of fabrics loaded with Echocho alcohol extract. The antiviral filter of the present invention can be used by loading the Echochocho alcohol extract in the effective concentration range having antiviral activity, preferably the filter is loaded with 1-100g / m ² Echochocho alcohol extract, more Preferably 5-50 g / m ² . The antiviral filter of the present invention may be a filter having a removal activity of the various viruses described above, and may be an influenza virus, which is preferably a human respiratory virus.

The filter of the present invention can be made in such a way that a variety of air permeable substrates are combined with Echochochoe extract. It can be performed during the fiber forming process, for example, a spun bond, melt blown nonwoven fabric.

As another aspect, the present invention relates to a method for producing a filter comprising the extract of Echochocho with antiviral activity disclosed above.

The method for preparing the filter of the present invention can be easily changed or applied by those skilled in the art according to the filter production method known in the art, preferably the filter of the Echochocho alcohol extract of the present invention loaded with 1-100g / m ² More preferably 5-50 g / m ² . In one embodiment of the present invention, Eochocho alcohol extract used a filter loaded with 8 g / m ² .

The filter of the present invention is prepared by a known electrospinning process in which an electrified liquid jet of polymer in the form of a solution or melt is formed and deposited on the pulverized collector fibers. Can be.

For example, in a preferred manufacturing method for preparing the filter material of the present invention for the face mask of the present invention, Echo extract can be incorporated into a liquid vehicle, after which the base material is wetted with the liquid composition to be formed and the substrate Wetting of the can be accomplished by simply coating the substrate material with the dispersion so formed, for example by immersing the substrate in the loading solution. Alternatively, the loading solution may be sprayed on the base material. On an industrial scale, spraying is preferred for convenience. The wetted substrate can then be dried, for example, by evaporation in ambient air or in a drying tunnel. Suitable drying temperatures in these tunnels are less than 100 ° C.

The loading solution usable in the method of making the filter of the present invention may be used without limitation various loading solutions known in the art.

For example, the loading solution may be prepared by adding 0.5 to 30% by weight, typically 5 to 10% by weight of edible grass ground hay in methanol or ethanol.

Thus, the filter material may be a product obtainable or obtained by a process of wetting the air permeable substrate with the loading solution, thereby causing or allowing evaporation of the liquid vehicle so that the material in the loading solution is deposited on the substrate. .

The filter material of the present invention can be easily produced with a lightweight mouth and / or nasal filter. In addition, the filter material of the present invention may be effective against pathogens such as the viruses mentioned herein.

In general, the filter material may be in the form of a sheet or pad that corresponds to the shape of the starting sheet or pad of the fibrous substrate, suitable for use in the aforementioned face mask. Such sheet or pad-like materials can be shaped to fit a face mask of generally known shape by known methods.

The influenza virus infection because the filter means or mask according to the present invention has the property of inhibiting the infectivity of the virus due to inhaled air contaminated with influenza virus or contaminated air extracted from a patient infected with the virus to prevent the occurrence of the disease It is expected to be of great help in preventing respiratory diseases.

1 is a block diagram of a mask comprising a fabric containing the Echochocho extract of the present invention.
Figure 2 is the activity curve of influenza virus used to measure the efficacy of the mask according to an embodiment of the present invention.
3 is a view comparing the virus activity in the liquid collected by collecting the air containing the virus passed through the mask material in the liquid phase according to an embodiment of the present invention compared with other mask material.
Figure 4 compares the virus activity in the liquid collected by trapping the air containing the virus passed through the mask treated with eochocho ethanol or eochochocho methanol extract in a liquid phase of the mask material according to an embodiment of the present invention and other mask materials One picture.
5 is a microscope comparing the virus active form in the liquid collected by collecting the air containing the virus passed through the mask material of the present invention in the liquid phase with another mask material. A: A state in which air that has not passed through the mask is collected in a liquid state and infected with cells, B: A state in which air that has passed through a mask made of a nonwoven material is infected in a liquid state, and C: A state of air that has passed through a general mask In the state of collecting the liquid and infecting the cells, D: The air passing through the N95 mask was collected in the liquid state and infecting the cells. E: The air passing through one sheet of nonwoven fabric containing the ethanol extract of Echoseongcho was collected in the liquid state. In the state of infection, F: air passed through two nonwoven fabrics containing ethanol extract in a liquid state, and infected with cells, G: air in two liquid non-woven fabrics containing methanol extract in a fishery, collected in a liquid state It shows the state infected with the cell.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the following examples are only for illustrating the present invention, and the content of the present invention is not limited by the following examples.

Reference Example  1: Influenza virus used in the test

Influenza virus (A / PR / 8/34, H1N1), a virus used in the present invention, was purchased from the American Cell Line Bank and infected with MDCK cells in the laboratory of the Korea Biotechnology Research Institute. After quantitatively displaying the viral activity in 10 ^-1 , 10 ^-2 , 10 ^-3 , 10 ^-4 (A), 10 ^-5 (B), and 10 ^-6 solutions obtained by 10-fold serial dilution, solution A and B The solution was applied to the mask efficacy evaluation of the mask of the present invention and other products.

Reference Example  2: mask used in the present invention and efficacy comparison

The degree of viral activity reduction was compared with the mask prepared in the present invention by purchasing three masks overlapped with a generic mask sold at a commercial pharmacy and an N95 mask sold by 3M.

Preparation Example 1 Methanol Extract of Eochocho

Crush the hay at the ground of Eosungcho and mix methanol 10 times more than the mass into a container, and place the container containing Eochocho hay and ethanol into the ultrasonic generator bath for 10 minutes at 40kHz and repeat this process five times to obtain methanol extract of Eochocho. Prepared. Thereafter, the resultant was filtered through a filter paper (Whatman No 2) to remove an insoluble substance, and the supernatant was dried to prepare a methanol extract.

Preparation Example 2 Ethanol Extract Preparation

Crush the hay at the ground of Eochocho and mix methanol 10 times the weight of hay into the container and place the container containing Eochocho hay and ethanol into the ultrasonic generator bath for 10 minutes at 40kHz and repeat this process 5 times. Was prepared. Thereafter, the resultant was filtered through a filter paper (Whatman No 2) to remove an insoluble substance, and the supernatant was dried to prepare a methanol extract.

Preparation Example 3 Preparation of Nonwoven Fabric Deposited with Methanol Extract

The nonwoven fabric was soaked in methanol extract of Eochocho for 24 hours, and then dried and dried for 16 hours at a temperature of 60 ° C in a drying tunnel. After drying, the mass of Echochochoea deposited on the nonwoven fabric was 8 g / m ² .

Preparation Example 4 Preparation of Nonwoven Fabric Deposited with Ethanol Extract of Echinacea

The nonwoven fabric was immersed in an ethanol extract solution of Eoseongcho for 24 hours, then dried and dried for 16 hours at a temperature of 60 ° C. in a drying tunnel. After drying, the mass of Echochochoea deposited on the nonwoven fabric was 8 g / m ² .

Example 1 Measurement of Influenza Virus Activity in a Gas Passed Through a Mask Loaded with Echochocho extract

Cut non-woven fabrics loaded with Echo extract, normal non-woven fabric mask, N95 mask, and a portion of the normal mask and cut them into each 25 mm diameter filter holder, and each filter holder was influenza virus A (A / PR) while passing air at a pressure of 2 psi. 3 ml of 0.4TCID50 solution (A) or 0.04TCID50 solution (B) of / 8/34, H1N1) were each sprayed into the air at a rate of 20 ml / h. After passing through the filter holder equipped with each mask, the air is collected in 30 ml of phosphate buffer solution, and the virus in the air is collected into the solution. The solution containing the virus is passed through a filter having a pore size of 0.22 μm and then aseptically treated. Each was administered to MDCK cells cultured in 96-well plates. After incubation for 48 hours, the results of the virus activity passed through the empty filter holder containing no mask material and the results of influenza virus activity in the air passing through each mask material were compared.

Determination of Influenza Virus Activity Through Each Mask Material Reduction in influenza virus activity (%) A solution B solution Virus infected cells that do not pass through the fabric 0.0 0.0 Plain mask 54.7 ± 7.3 88.6 ± 8.1 1 sheet of nonwoven fabric treated with Eosungcho ethanol extract + 1 sheet of ethanol nonwoven fabric 82,3 ± 14.2 92.2 ± 3.5 Two nonwoven fabrics treated with Eochocho ethanol extract 95.9 ± 0.9 96.2 ± 0.9 N95 mask 75.9 ± 17.1 94.4 ± 4.1

After reducing the virus activity after passing the gas containing influenza virus A solution (0.4TCID50) or B solution (0.4TCID50) through each mask material, 1 nonwoven fabric treated with Eochocho ethanol extract + 1 ethanol nonwoven fabric The mask material consisted of 82%, 3 ± 14.2% of solution A, and 92.2 ± 3.5% of solution B. The mask material consisting of two nonwoven fabrics treated with ethanol extract of Eungchocho was 95.9 ± 0.9 and solution B of B 96.2 ± 0.9% decreased. On the other hand, the normal mask showed a decrease of 54.7 ± 7.3% in solution A, 88.6 ± 8.1% in B solution, and 75.9 ± 17.1 in A solution and 94.4 ± 4.1% in B solution for N95 mask of 3M company. As a result, in each mask material which passed low concentration viral activity gas (B), there was no significant difference in virus activity reduction between masks, but when high activity virus containing gas (A) was passed, The N95 mask reduced virus activity from 88% to 54% and from 94% to 75%, whereas the nonwoven fabric loaded with Echo extract was 92% to 82% and 2 passes through 1 sheet. In the case of 96% to 95%, it showed excellent viral activity reduction compared to N95 mask.

Example  2: Eoseongcho Alcohol  Extract Loaded  Passed through the mask In gas  Microscopic Analysis of Influenza Virus Activity

In addition, the virus-containing gas was prepared by the method of Example 1 and collected after passing through each mask material to infect the MDCK cells, and then the air that did not pass through the mask was collected in the liquid phase to infect the cells ( A), as a result of collecting air passing through a mask made of a nonwoven material in a liquid state and infecting the cells (B), as a result of collecting air passing through a general mask in a liquid state and infecting the cells (C), passing through an N95 mask. As a result of collecting the air in the liquid phase and infecting the cells (D), as a result of collecting air passed through one sheet of nonwoven fabric loaded with Eochocho ethanol extract into the liquid and infecting the cells (E), the nonwoven fabric loaded with Eochocho ethanol extract As a result of collecting air passed through two sheets in a liquid phase and infecting the cells (F), the air passed through two sheets of nonwoven fabric loaded with methanol extract of fish oil was passed into the liquid phase. The results of collecting and infecting the cells (G) were observed under a microscope, respectively, and the results are shown in FIG. 5.

When the influenza virus is infected with a cell, a large amount of space (viral pathology) of the crushed cell is generated due to virus propagation after a certain time (A). As a result of collecting air passing through a mask made of a non-woven material in a liquid state and infecting the cells (B) and a result of collecting air passing through a general mask in a liquid state and infecting the cells (C), these cells are broken and empty. Although the space was reduced, a large number of viral pathologies have been observed. On the other hand, N95 mask (D) showing a decrease in virus activity of 70% or more, 1 non-woven fabric loaded with ethanol extract (E), 2 non-woven fabric loaded with ethanol extract (F), 2 non-woven fabric loaded with methanol extract (Echo) Intestinal (G) showed little viral pathology.

Example  3: Eochocho ethanol extract or methanol extract Loaded  Passed through the mask In gas  Influenza Virus Activity Assay

 Cut non-woven fabrics loaded with ethanol extract or methanol extract loaded with Echochocho extract, normal nonwoven mask, N95 mask, and a portion of the general mask and cut them into 25 mm diameter filter holders, and each filter holder was influenza virus A while passing air at a pressure of 2 psi. 3 ml of a solution (A / PR / 8/34, H1N1) having an activity of 25TCID50 was injected into the air at a rate of 20 ml / h. After passing through the filter holder equipped with each mask, the air is collected in 30 ml of phosphate buffer solution, and the virus in the air is collected into the solution. The solutions containing the virus are passed through a filter having a pore size of 0.22 μm, followed by aseptic treatment. The result of viral activity through the empty filter holder which was terminated after 48 hours of administration to MDCK cells cultured in 96-well plates and did not include the mask material, and the result of influenza virus activity in the air that passed through each mask material. By comparing the virus removal rate is shown in Table 2 below.

Reduction of influenza virus activity (%) Virus infected cells that do not pass through the fabric 0.00 Plain mask 46.6 ± 2.4 2 sheets of plain nonwoven 42.8 ± 4.2 1 nonwoven fabric treated with Eosungcho ethanol extract + 1 normal nonwoven fabric 75.9 ± 18.8 Two nonwoven fabrics treated with Eochocho ethanol extract 101.4 ± 29.5 1 sheet nonwoven fabric treated with Echoseong methanol extract + 1 sheet nonwoven fabric 37.3 ± 5.3 Two nonwovens treated with methanol extract of Eochocho 79.9 ± 11.7

As a result of measuring the decrease in virus activity after passing a gas containing influenza virus A solution (0.4TCID50) through each mask material, two normal nonwoven fabrics without loading the mask and Echo extract were 46% reduced in virus activity. And 42%, but the mask material consisting of 1 nonwoven fabric treated with Eochocho ethanol extract + 1 nonwoven fabric decreased 75% and the mask material consisting of 2 nonwoven fabrics treated with Echochocho ethanol extract was 101% Completely reduced viral activity. Mask material consisting of 1 nonwoven fabric + 1 nonwoven fabric treated with methanol extract of Eosungcho and one nonwoven fabric decreased 37%, while the mask material consisting of 2 nonwoven fabrics treated with methanol extract of Eochochos showed 79% viral activity. Therefore, it was confirmed that the nonwoven fabric loaded with Eochocho ethanol extract or Echochocho methanol extract had an effect of remarkably reducing viral activity when used as a mask material.

Claims (14)

An air permeable mask that is shaped on the user's mouth and nose and suitable for close contact with the patient's face, the device being provided with means for keeping the mask in place on the user's face and inhaling and / or evacuating air of the user At least one layer of filter material defined to pass through the filter material, wherein the filter material comprises an air permeable substrate loaded with an alcoholic extract of a fish herb bound to an acidic polymer.
The air permeable mask of claim 1, wherein the air permeable substrate comprises a fibrous substrate.
3. The air permeable mask of claim 2, wherein the air permeable substrate comprises a fabric of nonwoven fabric.
The air permeable mask according to claim 1, wherein the alcohol extract of the fish vinegar is an extract extracted with a solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, and mixed solvents thereof.
The air permeable mask of claim 1, wherein the mask prevents respiratory viral infections.
6. The air permeable mask according to claim 5, wherein said virus is an influenza virus.
The air permeable mask according to any one of claims 1 to 6, wherein the amount of the Echochocho alcohol extract is 1-100 g / m ² .
The air permeable mask according to any one of claims 1 to 6, wherein the substrate on which the alcohol extract of the fish herb is loaded is composed of singular or plural.
The method for manufacturing the air permeable mask according to claim 1.
Antiviral filter containing Echo extract.
11. The air permeable filter according to claim 10, wherein the filter is composed of a single or a plurality of fabrics loaded with alcohol extracts of fish vinegar.
The filter according to claim 10 or 11, wherein the filter has a loading amount of Eochochocho alcohol extract 1-100 g / m ² .
The filter of claim 10, wherein the virus is an influenza virus.
A method for producing the filter of claim 10.

Images