KR20120020708A

KR20120020708A - A method for producing processed ginger containing highly-concentrated [6]-shogaol using by novel treatment method

Info

Publication number: KR20120020708A
Application number: KR1020100084489A
Authority: KR
Inventors: 박찬우
Original assignee: 박찬우
Priority date: 2010-08-31
Filing date: 2010-08-31
Publication date: 2012-03-08

Abstract

The present invention relates to a method for producing processed ginger and an extract thereof containing a large amount of [6] -shogaol component through a novel treatment, and in particular, processed ginger and extract thereof prepared by the preparation method of the present invention. Silver [6] -shogaol, an active ingredient with strong anti-cancer, anti-inflammatory and anti-oxidant activity, is contained in a larger amount than ginger in the market, which is useful for medicines, cosmetics, and health functional foods that are expected to have anti-cancer, anti-inflammatory and anti-oxidant activities. Available.

Description

A method for producing processed ginger containing highly-concentrated [6] -shogaol using by novel treatment method} [6] -Shogaol (ｓｈｏｇａｏｌ)

The present invention relates to a process for producing processed ginger containing a large amount of [6] -shogaol component through a novel treatment method.

Document 1 Afzal M, Al-Hadidi D, Menon M, Pesek J, Dhami MS. Ginger: an ethnomedical, chemical and pharmacological review. Drug Metabol Drug Interact. 2001; 18 (3-4): 159-90.

[Reference 2] Chang CP, Chang JY, Wang FY, Chang JG. The effect of Chinese medicinal herb Zingiberis rhizoma extract on cytokine secretion by human peripheral blood mononuclear cells. J Ethnopharmacol. 1995; 48 (1): 13-9.

Reference 3 Thomson M, Al-Qattan KK, Al-Sawan SM, Alnaqeeb MA, Khan I, Ali M. The use of ginger (Zingiber officinale Rosc.) As a potential anti-inflammatory and antithrombotic agent. Prostaglandins Leukot Essent Fatty Acids. 2002; 67 (6): 475-8.

Ippoushi K, Azuma K, Ito H, Horie H, Higashio H. [6] -Gingerol inhibits nitric oxide synthesis in activated J774.1 mouse macrophages and prevents peroxynitrite-induced oxidation and nitration reactions. Life Sci. 2003; 73 (26): 3427-37.

[Reference 5] Surh YJ. Cancer chemoprevention with dietary phytochemicals. Nat Rev Cancer. 2003 Oct; 3 (10): 768-80.

[Document 6] Kim, Ji-ji, Ahn, Sang-il, Jeon-su Lee, Sae-mi Yoon, Mi-young Lee, Hyung-sun Yoon. Inhibition of cyclooxygenase-2 expression induced by Toll-like receptor 2, 3 and 4 agonists of 6-shogaol. Korean Society of Food Science and Technology. 2008; 40 (3): 332-6.

Reference 7 Sang S, Hong J, Wu H, Liu J, Yang CS, Pan MH, Badmaev V, Ho CT. Increased growth inhibitory effects on human cancer cells and anti-inflammatory potency of shogaols from Zingiber officinale relative to gingerols. Agric Food Chem, 2009; 57 (22): 10645-50

8 Dugasani S, Pichika MR, Nadarajah VD, Balijepalli MK, Tandra S, Korlakunta JN. Comparative antioxidant and anti-inflammatory effects of [6] -gingerol, [8] -gingerol, [10] -gingerol and [6] -shogaol. J Ethnopharmacol. 2010; 127 (2): 515-20.

The food contains various functional compounds that have anti-cancer and anti-inflammatory effects. One such food is ginger (Zingiber officinale Rosecoe) has been in binyong with herbal medicine, which traditionally has been reported to be effective for neurological disorders, toothache, gingivitis, stroke, asthma, constipation and diabetes. ^1).

Ginger extract is known to have anti-inflammatory ² ⁾ , antithrombotic ³ ⁾ , antioxidant ⁴ ⁾ and anticancer effects ⁵ ⁾ . Ginger has jinjeorol (gingerol), the show and includes all components such as (shogaol), paradol (paradol), ginger diol ^(gingerdiol) ^6). Among these, the shogaol component is reported to have superior physiological activity compared to other components.

According to a recent study, were the show indicate all compared to H-1299 human lung cancer cells, HCT-116 human grows stronger than jinjeorol in colorectal cancer inhibitory effect on the jinjeorol, in the IC ₅₀ [6] - shows that all the 8 microM, but [6] -gingerol reported that IC ₅₀ exhibited 150 microM, suggesting that [6] -shogaol showed stronger cancer growth inhibition, and [6] -shogaol was arachidonic It showed better activity than [6] -gingerol in acid secretion and inhibition of nitric oxide (NO). ⁷ ⁾ , [6] -shogaol [6] -gingerol, [8] ] -Gingerol and [10] -Gingerol have been reported to have stronger anti-inflammatory and antioxidant activity ⁸ ⁾ .

However, none of the above documents teaches or describes a method for producing processed ginger containing a large amount of the [6] -shogaol component through the novel treatment method.

Accordingly, the present inventors have developed a manufacturing method for preparing processed ginger containing a large amount of [6] -shogaol component, which is an active ingredient with strong anti-cancer, anti-inflammatory and antioxidant activity, as a component of ginger. The present invention was completed by confirming the advantages of obtaining excellent processed ginger.

In order to achieve the above object, the present invention is washed ginger at 70 ℃ to 150 ℃, preferably 90 ℃ to 120 ℃ 30 minutes to 24 hours, preferably 1 hour to 6 hours, more preferably 2 A first step of carrying out steaming for 4 to 4 hours to obtain first processed ginger; A second step of subjecting the processed ginger to a dry drying method or a hot air drying method, preferably a dry drying method at room temperature for preferably 6 hours to 1 week, preferably 12 hours to 48 hours to obtain a first processed ginger dried product; The first step and the second step process further comprises a three step process which is repeated several times, preferably 3 to 20 times, more preferably 4 to 15 times, even more preferably 5 to 10 times. To provide a manufacturing method for producing a processed ginger containing a large amount of [6] -shogaol (shogaol) component.

As defined herein, "evaporation (蒸法)" means "a method of cleaning a drug and then adding to it, such as alcohol, vinegar, or put it in a container without adding it to make it to a certain degree."

Hereinafter, the present invention will be described in detail.

The present invention is a ginger washed with water such as tap water at 70 ℃ to 150 ℃, preferably 90 ℃ to 120 ℃ 30 minutes to 24 hours, preferably 1 hour to 6 hours, more preferably 2 to 4 hours A first step of performing steaming for a period of time to obtain primary processed ginger; A second step of subjecting the processed ginger to a dry drying method or a hot air drying method, preferably a dry drying method at room temperature for preferably 6 hours to 1 week, preferably 12 hours to 48 hours to obtain a first processed ginger dried product; The third step process is repeated several times, preferably 3 to 20 times, more preferably 4 to 15 times, even more preferably 5 to 8 times, further repeating the first and second steps. It provides a manufacturing method for producing a processed ginger containing a large amount of the [6] -shogaol component, including.

[6] -shogaol component of the processed ginger when the first and second steps are repeated about 6 to 8 times in the third step of the manufacturing process is several times higher than that of ordinary ginger. ] -shogaol was confirmed to show an increase in the content.

In addition, the present invention can produce a ginger extract containing a large amount of the [6] -shogaol component from the processed ginger extract obtained from the production method.

Hereinafter, a method of obtaining a ginger extract of the present invention will be described in detail.

For example, the processed ginger obtained above is washed and dried to obtain water, a lower alcohol of C ₁ to C ₄ or a mixed solvent thereof, preferably a mixed solvent of water and methanol, as the extraction solvent, and dried ginger weight. About 1 to 5 times, preferably 2 times to 4 times of, and about 5 ° C. to 100 ° C., preferably 20 ° C. to 90 ° C., more preferably at room temperature, about 12 hours to 2 weeks, preferably Preferably, ultrasonic extraction is performed for about 30 minutes to 12 hours, preferably 1 to 2 hours after primary extraction by cold extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction or heat extraction, preferably cold extraction for 72 hours. After performing the step of filtration and concentration under reduced pressure, a ginger extract containing a large amount of the [6] -shogaol component of the present invention can be obtained.

The processed ginger prepared by the above method and its extract contain anti-cancer, anti-inflammatory and antioxidant since it contains more [6] -shogaol component, which is a potent active ingredient of anti-cancer, anti-inflammatory and antioxidant activity, than ginger in the market. It will be useful for the development of pharmaceuticals, cosmetics and dietary supplements that are expected to be active.

As described above, the processed ginger prepared by the preparation method of the present invention and its extract have a greater amount of [6] -shogaol component, which is an active ingredient with strong anti-cancer, anti-inflammatory and antioxidant activity, than the commercially available ginger. It will be useful in the development of medicines, cosmetics and health functional foods that are expected to have anti-cancer, anti-inflammatory and antioxidant activity.

Figure 1 is a diagram showing the preparation of ginger sample for each step,
2 is a view showing the change in the content of 6-shogaol according to the number of increase,
3 is a view showing the change in the content of 6-gingerol according to the number of increase
4 is a view showing the change in the content of 8-gingerol according to the number of increases
5 is a view showing the change in the content of 10-gingerol according to the number of increases.

Hereinafter, the present invention will be described in detail. However, the following Examples, Reference Examples and Experimental Examples are merely illustrative of the present invention, but the content of the present invention is not limited thereto.

Example 1 Method of Treating Ginger by the Steaming Method

1-1.Preparation of Standards

Standards of ginger include [6] -shogaol, [6] -gingerol, [8] -gingerol, and [10] contained in one KIT (product no. 00007615). ] -Gingerol was purchased from the company (ChromaDex, Santa Ana, CA, USA) and used for the experiment.

1-2. Increase step by step Ginger Sample Ready

: Fresh ginger (improved species) washed with tap water was purchased from Hwang Saengwon (Wonju, Jeonbuk; www.gingerkorea.com ) and subjected to steaming with 3 repetitions of 5 kg each. 5 kg of fresh ginger was added at 100 ° C. for 3 hours for 1 hour, and then dried for 24 hours at room temperature. Among them, 100 g was taken and stored as 1 ginger. In the next step, 100 g of dried powder was added in the same manner as above. Ginger was stored as shown in FIG. 1 (steps referred to as step 1, ginger 1, ginger 2, etc.).

1-3. Extraction Method :

The processed ginger prepared in step 1-2 and commercially sold ginger (control group; modified fresh ginger purchased from Hwang Saeng Won) were each subdivided into a grinder, each taken 1 g each, immersed in 100 ml of MeOH, and extracted for 72 hours at room temperature. Sonication (S900H, Elma, Germany). The extract was filtered with a filter paper (0.2um syringe filter; Millipore Corporation, Bedford, Mass., USA) and used as an UPLC analysis sample.

Experimental Example One. In the law Changes in Surface Components of Ginger

The change in the contents of the indicator components, namely, [6]-, [8]-, [10] -gingerol and [6] -shogaol, contained in the processed ginger extract and the control ginger extract obtained in the above example were analyzed as follows. It was.

1-1. Experiment process and condition

1 ml of the extract sample was used with an ACQ 2996 PDA detector and an ACQUITY UPLC BEH C18 column (1.7 μm, 100 mm × 2.1 mm, USA) under UPLC (ACQUITY ^™ Ultra Performance LC system; Waters, Milford, MA, USA) under the following conditions: The content of indicator components was analyzed under a gradient of 0.1% formic acid containing distilled water (Formic acid in water) and 0.1% formic acid containing acetonidinyl (0.1% Formic acid in ACN) solvent concentration (see Table 1).

Solvent gradient for the analysis of gingerols and 6-shogaol Time (min) Flow rate
(Ml / min) 0.1% formic acid in DW 0.1% formic acid in AcCN One Early 0.4 98 2 2 One 0.4 98 2 3 2 0.4 90 10 4 7 0.4 50 50 5 8 0.4 20 80 6 10 0.4 0 100 7 11 0.4 98 2 8 12 0.4 98 2

As a result, the contents of [6]-, [8]-, and [10] -gingerol gradually decreased by the treatment of ginger, but the content of [6] -shogaol continuously increased up to 6 years. However, after 6, there was no increase in content anymore.

In addition, [6] -shogaol was 320.0 in normal ginger, 945.0 in 1 ginger, 1458.3 in 2 ginger, 1961.7 in 3 ginger, 2998.3 in 4 ginger, 3410.0 in 5 ginger, 3930.0 (ppm) in 6 ginger. The maximum content was found in 6 cases, and the content of [6] -shogaol was 12.3 times that of normal ginger. 7 ginger ginger 3910.0, 8 ginger ginger 3760.0, 9 ginger ginger 3510.0 (ppm) showed no increase in content.

In conclusion, the treatment method of maximizing the content of [6] -shogaol in ginger was confirmed that the treatment method of 6 or 7 was 'the ginger manufacturing method of maximizing the content of [6] -Shogaol' (see FIGS. 2 to 5). ).

Claims

A first step of performing the washed ginger at 70 ° C. to 150 ° C. for 30 minutes to 24 hours to obtain first processed ginger; Performing a dry drying method or a hot air drying method on the processed ginger to obtain a first processed ginger dried product; Method for producing a processed ginger containing a large amount of the [6] -shogaol component, comprising a third step of repeating the first step and the second step three to 20 times .

The method of claim 1,
Method for performing steaming (蒸法) for 2 hours to 4 hours at 90 ℃ to 120 ℃ in the first step.

The method of claim 1,
Method for performing a dry drying method at room temperature for 12 to 48 hours in the second step.

The method of claim 1,
Method of repeating the first step and the second step in the third step 5 to 10 times further.