KR20120016345A - System for signal detection of specimen using magnetic resistance sensor and detecting method of the same - Google Patents

Abstract

PURPOSE: Contactless detecting system and method using a magnetic reluctance sensor are provided to enhance the reliability of measurement since the detecting positions of a magnetic reluctance sensor and a subject are maintained quantitively constantly. CONSTITUTION: A detecting system using a magnetic reluctance sensor comprises a subject fixing unit, a detector(200), a system control unit(300), and a transfer module. The subject fixing unit comprises a subject(121) coupled to a magnetic particle and a reference magnetism band(122) consisting of a pure magnetic particle. The detector senses magnetic signals generated by application of magnetic field to the subject fixing unit with a magnetic reluctance sensor(130), divides the signals into magnetic components and analyzes the components. The system control unit compares the reference magnetism signal for the pure magnetic particle generated from the detector with the detected magnetic signal for the subject.

Description

System for signal detection of specimen using magnetic resistance sensor and Detecting Method of the same}

The present invention relates to a high sensitivity inspection system and method for quantitatively measuring magnetic particles using a magnetoresistive sensor.

In general, a magnetic sensor cartridge (magnetic sensor) is a sensor cartridge for measuring the size and direction of the magnetic field or the line of magnetic force, the magnetic field is measured by changing the properties of various materials due to the influence of the magnetic field. Hall elements or magnetoresistive (MR) elements are made using the Hall effect, magnetoresistance effect, etc., and they are also used to manufacture VTRs (Video Tape Recorders) and tape recorders. The substance affected by the magnetic field is basically to apply a magnetic field to the detection object coupled to the magnetic particles to detect the change in the magnetic field.

The above-described magnetic particles are manufactured from magnetite, and have been studied and used for a long time in the field of biochemistry, because they are very promising as tags for detecting the presence of bound objects by coating the surface of the particles with a biomaterial of interest. come. However, since magnetic signals from very small volumes of magnetic particles are extremely small, the manufacture of magnetic detectors remains a difficult challenge and is being studied in various ways.

Apparatus and method related to this are based on Superconducting Quantum Interference Devices (hereinafter referred to as “SQUID”), but due to the general complexity and cryogenic elements of SQUID, This method is not suitable. More traditional methods include some form of force magnetometer, but there are limitations to quantitative or high sensitivity measurements. Thereafter, as a method for quantitatively measuring local accumulation of magnetic particles, a method using a hall sensor or a giant magneto-resistance (hereinafter referred to as “GMR”) sensor has been proposed. However, there are many limitations to the practical use in terms of inaccuracy of sensitivity and construction of measurement equipment.

1 is a conceptual diagram illustrating a sensing principle of a magnetoresistive sensor used in the present invention. However, for convenience of description, the sensing principle using a giant magneto resistance (GMR) of the magnetoresistive sensor will be described as an example.

This shows a spin-valve type Giant Magneto Resistance (GMR) device. As shown, the magnetoresistive sensor has a nonmagnetic metal layer sandwiched between two ferromagnetic metal layers. The magnetic force of the ferromagnetic metal layer of the first layer is fixed, and the magnetic force of the ferromagnetic material of the second layer is variably adjusted. When the first layer and the magnetic force are parallel, the principle that only electrons spin-oriented in a specific direction passes through the conductor. That is, according to the alignment of the magnetization directions of the two ferromagnetic layers, a difference in electrical resistance, or a potential difference, induced inside the material is generated and recognized as a digital signal. The case where an interlayer material is a conductor corresponds to a GMR device.

The method of measuring the intensity of quantitative magnetic force of a subject with a magnetic material in the uniform magnetic field area in which the magnetic field is distributed includes a contact method and a subject in which the subject and the magnetoresistive sensor are in contact with each other to measure the strength of the magnetic force. There are two ways to measure with a certain distance.

First, in the method of measuring the sample to be measured by contacting with the magnetoresistive sensor, it is impossible to know exactly whether the magnetoresistance sensor and the measurement object are in contact, and the magnetoresistance sensor may be damaged due to the shock generated during long time contact. Measurement should be made uniformly, but the resistance of the magnetoresistive sensor is frequently distorted due to the impact caused by contact, which causes the distance between the magnetoresistive sensor and the specimen, which causes a problem of reliability of measurement. In addition, since the magnetic material remains in the sensor, it is necessary to clean the magnetic material with other things, which causes a problem of adding a separate process and equipment.

In addition, in the case of the non-contact type, the measurement distance from the sample to be measured spaced apart from the magnetoresistive sensor cannot be set uniformly, thereby causing a problem in that the reliability and uniformity of the detection cannot be guaranteed.

The present invention has been made to solve the above problems, an object of the present invention is to use a magnetoresistive sensor and a magnetic resistance sensor and a sample in a diagnostic device for measuring the sample quantitatively using magnetic particles accumulated in the side flow membrane It is possible to maintain the detection position of the quantitatively and quantitatively, thereby increasing the reliability of the measurement, and further enabling the non-contact quantitative measurement, and the reliability of the measurement due to the breakage and damage of the magnetoresistive sensor due to the impact of the conventional contact method. It is to provide a diagnostic device and method that can implement a quantitatively uniform measurement while maintaining the reliability of the measurement without problems and separate cleaning device.

As a means for solving the above problems, the present invention provides a sample fixing unit comprising a reference magnetic band consisting of a sample and the pure magnetic particles coupled to the magnetic particles; A detector for detecting a magnetic signal generated by applying a magnetic field to the specimen fixing unit with a magnetic resistance (MR) sensor and separating and analyzing the magnetic signal into magnetic components; It provides a detection system using a magnetoresistive sensor comprising a; system control unit including a signal processing unit for setting the reference position of the measurement by comparing the reference magnetic signal for the pure magnetic particles generated by the detector with the detection magnetic signal for the sample; Do it.

The above-described detection system may further include a transfer module for transferring the specimen fixing unit to the detection area of the magnetoresistive sensor.

In addition, the system control unit in the above-described detection system, it may be implemented to control to set the point where the intensity of the magnetic signal is rapidly changed compared to the reference magnetic signal in the specimen fixing unit to the measurement reference position.

In addition, the signal processing unit according to the present invention, the amplification unit for amplifying a reference magnetic signal or a measurement magnetic signal input from the magnetoresistive sensor; And an AD converter for converting an analog value of the amplified signal into a digital value. The AD converter may be driven to store a signal passed through the amplifier and the AD converter in the system controller.

The signal processing unit may further include a filter unit which removes high frequency or low frequency noise included in the amplified signal, and after passing the amplified signal of the reference magnetic signal or the measured magnetic signal through the filter unit, the AD converter unit. It can be implemented to be driven to be stored in the system control unit via.

In addition, the amplifying unit in the detection system according to the present invention, at least one amplifying unit for amplifying the detection signal; and at least one low pass filter unit for removing noise of the magnetic signal amplified by the amplifying unit or It may be configured to include; high-pass filter unit.

In addition to the above-described configuration, the system controller according to the present invention includes a peak detector for detecting a peak value of the reference magnetic signal or the measured magnetic signal; An analysis unit analyzing the peak value; And an arithmetic circuit unit for deriving a calculation value for the signal through comparison operation based on the analyzed signal information.

In any case, the detector in the detection system according to the present invention includes: a magnetoresistive sensor for detecting a magnetic component of a sample to which magnetic particles are coupled; and an external magnetic field applying device for applying an external magnetic field to the magnetoresistive sensor; . ≪ / RTI >

In this case, the external magnetic field application device includes: a first application unit for applying a magnetic field to the magnetoresistive sensor in a horizontal direction; And a second application unit applying a magnetic field to the magnetoresistive sensor in a vertical direction.

In addition, the magnetoresistive sensor provided in the detector of the present invention may be configured as a giant magnetoresistance (GMR) sensor.

In particular, the specimen fixing unit according to the present invention may be configured as a measurement cartridge or membrane in which the biomaterial containing the antigen is fixed.

By using the detection system according to the present invention described above it is possible to detect a sample in the following steps.

Specifically, the first step of measuring the reference magnetic signal of the pure magnetic particles of the sample fixing unit including a reference magnetic band consisting of the sample and the magnetic particles combined with the magnetic particles with a magnetoresistance sensor; Measuring a detection magnetic signal generated by a magnetic field applied to the specimen with a magnetoresistive sensor; And a three step of setting a point where the intensity of the detected magnetic signal is sharply changed in comparison with the reference magnetic signal as a measurement reference position by a system controller.

In this case, the first and second steps may be formed by approaching and measuring the magnetoresistive sensor through a transfer module for transporting the specimen fixing unit in the region to which the magnetic field is applied, and storing the sample in the system controller. have.

The reference magnetic signal or the detected magnetic signal of the first and second steps may further include storing the magnetic signal measured by the magnetoresistive sensor to the system control unit through an amplifier and an AD converter. have.

In the above-described detection step, the reference magnetic signal or the detection magnetic signal of the first and second steps may be provided to the system control unit through an amplification unit, a filter unit for removing noise, and an AD converter unit from the magnetic signal measured by the magnetoresistive sensor. The method may further include storing.

In addition, after the three steps of the above-described detection step, it may further comprise a four step of detecting the sample by the method of horizontally scanning the sample fixing unit at the measurement reference position.

Of course, in this case, after the step 3, it may be implemented to further include a four step of detecting the sample by moving the sample fixing unit in a horizontally spaced position in the downward direction from the measurement reference position by scanning Do.

In any case, the measurement reference position is preferably formed in a range that does not contact the magnetoresistive sensor.

According to the present invention, in the diagnostic device for measuring the sample quantitatively by using the magnetic particles accumulated in the side flow membrane using the magnetoresistance sensor, the detection position between the magnetoresistive sensor and the sample can be kept quantitatively constant. There is an effect that can increase the reliability of.

In addition, by enabling non-contact quantitative measurement, it is possible to implement a quantitatively uniform measurement while maintaining the reliability of measurement without a separate cleaning device and the reliability problem of measurement due to damage and damage of the magnetoresistive sensor due to the impact of the conventional contact method. There is also an effect.

In addition, by utilizing a non-contact giant magneto-resistance sensor (Giant Magneto Resistance) it is possible to perform efficient bio-diagnosis through sensing the sample. Therefore, the membrane used for the point of care testing (POCT) can be installed in the sample diagnostic kit to develop a measuring instrument for effective membrane measurement.

In addition, by applying a DC magnetic field in the Z-axis direction to the magnetoresistive sensor, the superparamagnetic magnetic particles are saturated magnetized, and by applying a magnetic field in the Y-axis direction, the sensitivity performance of the magnetoresistive sensor can be maximized.

1 is a conceptual diagram illustrating a sensing principle of a magnetoresistive sensor used in the present invention.
2 is a block diagram illustrating a detection system using a magnetoresistive sensor according to the present invention.
Figure 3 shows another embodiment of the configuration of the signal processing unit according to the present invention.
4A and 4B show a conceptual diagram and a configuration block for explaining the operation of the detection system according to the present invention described above.
5A to 5D are tables showing verification results for experimenting with validity of data when setting the reference position in FIG. 4A and measuring position by slightly moving down from the reference position as described above.

Hereinafter, with reference to the accompanying drawings will be described in detail the configuration and operation according to the present invention. In the description with reference to the accompanying drawings, the same components are given the same reference numerals regardless of the reference numerals, and duplicate description thereof will be omitted. The terms first, second, etc. may be used to describe various components, but the components should not be limited by the terms. The terms are used only for the purpose of distinguishing one component from another.

The present invention is to maintain a constant reference position corresponding to the measurement position between the magnetoresistive sensor and the sample to be measured, the problem of reliability of the measurement due to damage and twisting of the magnetoresistive sensor due to the impact of the contact method and separate cleaning It is an object of the present invention to provide a system and method capable of measuring quantitatively and uniformly while maintaining the reliability of measurement without the need for an apparatus.

1 is a conceptual diagram illustrating a sensing principle of a magnetoresistive sensor used in the present invention. However, for convenience of description, the sensing principle using a giant magneto resistance (GMR) of the magnetoresistive sensor will be described as an example.

This shows a spin-valve type Giant Magneto Resistance (GMR) device. As shown, the magnetoresistive sensor has a nonmagnetic metal layer sandwiched between two ferromagnetic metal layers. The magnetic force of the ferromagnetic metal layer of the first layer is fixed, and the magnetic force of the ferromagnetic material of the second layer is variably adjusted. When the first layer and the magnetic force are parallel, the principle that only electrons spin-oriented in a specific direction passes through the conductor. That is, according to the alignment of the magnetization directions of the two ferromagnetic layers, a difference in electrical resistance, or a potential difference, induced inside the material is generated and recognized as a digital signal. The case where an interlayer material is a conductor corresponds to a GMR device.

2 is a block diagram illustrating a detection system using a magnetoresistive sensor according to the present invention (hereinafter referred to as a 'detection system').

The detection system according to the present invention includes a sample fixing unit 120 including a sample 121 coupled to magnetic particles and a reference magnetic band 122 composed of pure magnetic particles, and applying a magnetic field to the sample fixing unit 120. The detector 100 detects the magnetic signal generated by the magnetic resistance (MR) sensor 130 and separates the magnetic signal into magnetic components, and the reference magnetic signal and the sample for the pure magnetic particles generated by the detector. And a system controller 300 including a signal processor 200 for setting a measurement reference position by comparing the detected magnetic signals.

In particular, the specimen fixing unit 120 according to the present invention includes a structure that can be moved by the moving module (M) to be moved into the detection area of the detector 100 to receive an external magnetic field. If the structure is provided with a reference magnetic band 122 that is spaced apart from the sample to be detected (magnetic particles + biomaterial), any one of the structures may correspond to the sample fixing unit of the present invention, but in one preferred embodiment of the present invention An example using a cartridge or membrane will be described.

The detector 100 according to the present invention includes a magnetoresistance (MR) sensor 130 and a first direction and a second direction of the magnetoresistive sensor 130, which detect magnetic components of a sample 122 to which magnetic particles are coupled. And an external magnetic field applying device 110 for applying a directional external magnetic field.

Particularly, the external magnetic field applying device includes a first application unit 111 for applying a magnetic field to the magnetoresistive sensor 130 in a horizontal direction that is a first direction, and a magnetic field in a vertical direction that is a second direction to the magnetoresistive sensor 130. It includes a second applying unit 112 for applying. Mounting the sample 121 to the sample fixing unit 120 through this basic structure, and applying an external magnetic field from the external magnetic field applying device 110, the magnetic component (magnetic particles) from the magnetoresistive sensor 130 ) Detects the magnetic signal of the sample combined with the) so that it can be separated and analyzed into electrical components. Magnetic particles may have a magnetization value of 10 ~ 100emu / g. In this case, the magnetic particles may have superparamagnetism or paramagnetism. As described above, the detection device uses external application devices 111 and 112 for applying a magnetic field to the magnetoresistive sensor in at least one direction so that the intensity of the magnetic force received by the magnetic particles in the biomaterial is the sum of the applied magnetic fields in the horizontal and vertical directions. Therefore, it is possible to improve the sensitivity by increasing the magnetization power of the biomaterial.

The magnetic signal detected by the magnetoresistive sensor 130 is amplified by the amplifying unit 210, and is converted into a digital signal by the AD converter 220 through signal processing such as noise removal to be stored as a signal. Such signal processing is performed by the signal processor 200, and the signal processor may be formed in various configurations.

For example, as shown in FIG. 2, the amplifier includes an amplifier 210 for amplifying a magnetic signal input from the magnetoresistive sensor 130 and an AD converter 220 for converting an analog value of the amplified signal into a digital value. The amplification unit and the AD converter may be configured to store the signal through the system control unit 300. Alternatively, as illustrated in FIG. 3, the amplification unit 210 and the AD converter 220 may be amplified. At least one low pass filter unit or at least one high pass filter unit for removing noise of the magnetic signal amplified by at least one amplifying unit which removes high frequency or low frequency noise included in the signal, or a magnetic signal Peak detection unit 240 for detecting the peak value of the, the analysis unit 250 for analyzing the peak value, arithmetic circuit unit for deriving the calculated value for the signal through a comparison operation based on the analyzed signal information ( It may also be implemented in a configuration that further includes 260.

4A and 4B show a conceptual diagram and a configuration block for explaining the operation of the detection system according to the present invention described above.

Figure 4a shows a conceptual diagram for explaining the operation of the detector and the sample fixing unit according to the present invention.

In the detection method of the present detection system, first, the specimen fixing unit 120 in the transfer device M is transferred into the magnetic field applying region inside the external magnetic field applying devices 111 and 112.

In this case, the specimen fixing unit 120 preferably has a structure in which a reference magnetic band 122 composed of a specimen 121 and pure magnetic particles coupled to the magnetic particles on the upper portion of the measurement cartridge.

First, the reference magnetic band 122 is moved to the position of 'I' by transferring the sample fixing unit 120 in the direction of '1' from the position of 'A', which is the initial position, and again from the position of 'I'. In the direction of '2' will move to the position 'da'. When the sample fixing unit 120 is located at the position of 'c', the amplifying unit 210 of FIG. 2 detects the amplified signal with a magnetoresistive sensor, and the current magnetic signal (the reference) is converted by the AD converter 220. Magnetic signal) and the system controller 300 stores the reference magnetic signal value.

The system controller 300 stores the reference magnetic signal value, and then increases the sample fixing unit 120 little by little until it comes to the position of 'la' in the direction of '3' from the position of 'da'. The magnetoresistive sensor detects the magnetic signal value (detection magnetic signal) of the specimen 121 of the specimen fixing unit 120, and converts the signal amplified by the amplifier 210 in FIG. 2 into the AD converter 220. If the system controller 300 continues to compare with the value (reference magnetic signal) stored in 'da' and increases the value, the magnetic signal value increases rapidly when it comes to a specific point (d). Using (d) as a reference (measurement reference position), measurement is performed while scanning the specimen fixing unit from side to side.

Of course, in this case, unlike the case where the scanning process at the measurement reference position, the sample fixing unit 120 is lowered to a position of 'e' in the direction of '4' by moving the sample 121 to the left or right. You can also measure while scanning.

FIG. 4b shows as an embodiment the detector according to the invention in FIG. 4a and the detection system described in FIG.

The same reference numerals represent the same components, and the sample fixing unit 120 moves to the inside of the detector 100 to detect the sample according to the above-described process.

In this case, the signal processor which processes the value of the reference magnetic signal or the detected magnetic signal generated by the magnetoresistive sensor 130 further includes an amplifier including a plurality of amplifiers AMP and a plurality of filters 231, 232, and 233. In this case, a process of directly converting the value of the reference magnetic signal or the detected magnetic signal amplified by the first amplifier 211 in the ADC converter 220 and storing and comparing the same in the system controller 300 is performed. As shown in FIG. 4B, the signal after passing through the filter units 231, 232, and 233 may be converted by the ADC converter 220 to be stored and compared in the system controller 300.

Table 1 below shows actual data values for finding the position of the reference position 'D' in FIGS. 4A and 4B.

{Table 1}

Moving in the direction of '3' in the position 'da' of FIG. Set this point to the position of 'la', and use the Six sigma verification tool, which is a statistical verification tool, to move the position of 'horse' in FIG. 4a according to the distance and concentration of the position of 'horse' from the position of 'la' This will affect the reliability of the. (You may need to add a semantic description of this part. A description of how moving down from the reference position affects reliability.)

5A to 5D are tables showing verification results for experimenting with validity of data when setting the reference position in FIG. 4A and measuring position by slightly moving down from the reference position as described above.

Magnetic particle concentration was 0.25%, the moving interval was measured at 150 pulse intervals (Fig. 5a), 200 pulse intervals (Fig. 5b), 250 pulse intervals (Fig. 5c), 300 pulse intervals (Fig. 5d). The validity of the data was verified using the Six sigma verification tool, which is a validation tool, according to the distance and concentration moving from the position of 'la' to the position of the 'horse' of FIG. 4a.

The verification results of FIG. 5A are shown in Table 2 below.

{Table 2-Two-Sample T-Test}

Through the results of Table 2 above, using the non-contact method of FIG. 4A, when the sample having the same concentration is repeatedly measured at 150 pulses away from the measurement reference point (d), it is confirmed that the data is reliable in the 95% confidence interval. Can be.

The verification results of FIG. 5B are shown in Table 3 below.

Table 3-Two-Sample T-Test

Through the results of Table 3, it can be seen that the data is reliable in the 95% confidence interval when repeated measurements at 200 pulses away from the measurement reference point (d) using the non-contact method of FIG. 4a. Can be.

The verification results of FIG. 5C are shown in Table 4 below.

{Table 4}

Through the results of Table 4, it can be seen that the data is reliable in the 95% confidence interval when repeated measurements at 250 pulses away from the measurement reference point (d) using the non-contact method of Figure 4a Can be.

The verification results of FIG. 5D are shown in Table 5.

{Table 5}

Through the results of Table 5, it can be seen that the data is reliable in the 95% confidence interval when repeated measurements at 300 pulses away from the measurement reference point (d) using the non-contact method of Figure 4a Can be.

The above-described detection method is a quantitative measurement and measurement in measuring the magnetic material in the device capable of lateral flow of magnetic particles combined with a sample (target molecule) in a device using a magnetoresistive sensor for quantitative measurement of magnetic material In order to improve the reliability of the sample, the sample fixing unit including the reference magnetic band mounted on the sample (magnetic material + bio material) and the constant concentration (magnetic material) material to be measured It becomes possible to grasp the optimum measuring position and the range of detection as the distance which is separated from the measuring reference position or the measurement reference position by a portion.

In particular, the detection method using the specimen fixing unit has the advantage that it is possible to uniformize the measurement reliability roll according to the measurement deviation between the devices, many measuring devices of the same type, and the method and principle of measuring magnetic materials It can be applied to a device for measuring a sample containing magnetic material.

Hereinafter, the configuration of the detector of the present invention shown in Figure 4a will be described in detail.

The magneto-resistive sensor 130 of FIG. 4A is configured to apply an external magnetic field in the first direction and the second direction, and the first direction serves to create a horizontal magnetic field to hold the initial position of the magnetoresistive sensor. It can improve the performance of the sensor by blocking the noise and surrounding noise.

Specifically, the first direction and the second direction are particularly preferably provided in the first application unit 111 for applying the magnetic field in the horizontal direction (Y-axis) which is the first direction to the magnetoresistive sensor, and the magnetoresistive sensor. It is preferable to include a second application unit 112 for applying a magnetic field in the vertical direction (Z axis) in two directions. Of course, the above-mentioned vertical direction in the horizontal direction does not necessarily mean only perpendicular to the elevation of the magnetoresistive sensor, but includes a concept that includes a certain degree of fluidity in the incident direction. In addition, the second application unit 112 is preferably implemented to be able to change the magnetic field through the current. The range of the horizontal (Y-axis) magnetic field or the range where the magnetoresistive sensor (MR) can react is formed at 2 to 30 gauss, and the magnetic field applied to the vertical direction (Z-axis) is 1200 to 1800 gauss ( Gauss) can be formed in the range.

The GMR sensor is very strong only in the magnetic field perpendicular to the sensor (Y axis) and slightly in the direction parallel to the sensor (X axis), while in the direction perpendicular to the sensor (Z axis). It is not affected at all. In addition, for the magnetic field in the Y-axis direction, it is possible to adjust the bias (biasing) within a unique linear range (linear range).

Therefore, the system design for the maximum performance of the GMR sensor is applied by applying a DC magnetic field in the Z-axis direction to saturate the superparamagnetic magnetic particles and applying the magnetic field in the Y-axis direction to improve the sensitivity performance of the sensor. It is essential to have maximum deflection control. In this case, the application of the magnetic field in the Y-axis direction is very effective in improving the signal-to-noise ratio by using an induced magnetic field generated through a DC current.

In addition, the first application unit 111, the magnetic field generating unit may be implemented in a configuration for applying a fixed magnetic field consisting of any one or more selected from the solenoid coil, Helmholtz coil, electromagnet yoke, permanent magnet. The second application unit 112 may include any one or a plurality of magnetic field generating units selected from solenoid coils, Helmholtz coils, and electromagnet yokes.

In addition, the magnetoresistive sensor 130 of the present invention is a normal magnetoresistance (OMR) sensor, anisotropic magnetoresistance (AMR) sensor, a giant magnetoresistance (GMR) sensor, a super-magnetism ( Colossal Magnetoresistance (CMR) sensor, Tunneling Magnetoresistance (TMR) sensor, MJT (Magnetic Tunneling Junction) sensor, Planar Hall Resistance (Planar Hall Resistance) sensor is preferably used any one selected from. Particularly preferably, a giant magnetoresistance (GMR) sensor may be used.

In the foregoing detailed description of the present invention, specific examples have been described. However, various modifications are possible within the scope of the present invention. The technical idea of the present invention should not be limited to the embodiments of the present invention but should be determined by the equivalents of the claims and the claims.

110: external magnetic field application device
111: first authorization unit
112: second authorization unit
120: specimen fixing unit
121: sample (sample)
122: reference magnetic band
130: magnetoresistive sensor
200: signal processing unit
210: amplification unit
220: AD conversion unit

Claims (18)

A sample fixing unit including a reference magnetic band consisting of a sample coupled to the magnetic particles and pure magnetic particles;
A detector for detecting a magnetic signal generated by applying a magnetic field to the specimen fixing unit with a magnetic resistance (MR) sensor and separating and analyzing the magnetic signal into magnetic components;
And a system control unit including a signal processing unit configured to set a measurement reference position by comparing the reference magnetic signal for the pure magnetic particles generated by the detector with the detection magnetic signal for the sample.
The method according to claim 1,
The detection system,
And a transfer module for transferring the specimen fixing unit to the detection area of the magnetoresistive sensor.
The method according to claim 2,
The system control unit,
And the specimen fixing unit controls to set the point where the intensity of the magnetic signal changes sharply in comparison with the reference magnetic signal as a measurement reference position.
The method according to claim 3,
The signal processing unit,
An amplifier for amplifying a reference magnetic signal or a measured magnetic signal input from the magnetoresistive sensor;
And an AD conversion unit for converting an analog value of the amplified signal into a digital value.
And a magnetoresistive sensor driven to store a signal passed through the amplifier and the AD converter in the system controller.
The method of claim 4,
The signal processing unit,
And a filter unit for removing high frequency or low frequency noise included in the amplified signal.
And a magnetoresistive sensor driven to store an amplified signal of the reference magnetic signal or the measured magnetic signal after passing through the filter unit and stored in the system control unit via the AD converter.
The method according to claim 5,
The amplification unit,
At least one amplifier for amplifying the detection signal; Wow
At least one low pass filter or high pass filter for removing noise of the magnetic signal amplified by the amplifier;
Detection system using a magnetoresistive sensor configured to include.
The method according to claim 4 or 5,
The system control unit,
A peak detector detecting a peak value of the reference magnetic signal or the measured magnetic signal;
An analysis unit analyzing the peak value; And
And a calculation circuit unit for deriving a calculation value for the signal through comparison operation based on the analyzed signal information.
The method according to any one of claims 1 to 6,
The detector device,
Magnetoresistance sensor for detecting the magnetic component of the sample to which the magnetic particles are coupled; Wow
An external magnetic field applying device for applying an external magnetic field to the magnetoresistive sensor; Including
System using a magnetoresistive sensor.
The method according to claim 8,
The external magnetic field applying device,
A first application unit for applying a magnetic field to the magnetoresistive sensor in a horizontal direction;
A second application unit for applying a magnetic field to the magnetoresistive sensor in a vertical direction;
Detection system using a magnetoresistive sensor comprising a.
The method according to claim 9,
The magnetoresistive sensor is a detection system using a magnetoresistive sensor which is a giant magnetoresistive (GMR) sensor.
The method according to claim 10,
The sample fixing unit,
Detection system using a magnetoresistance sensor, which is a measurement cartridge or membrane in which a biomaterial containing an antigen is fixed.
Measuring a reference magnetic signal of a pure magnetic particle of a sample fixing unit including a reference magnetic band including a sample coupled to the magnetic particles and pure magnetic particles with a magnetoresistive sensor;
Measuring a detection magnetic signal generated by a magnetic field applied to the specimen with a magnetoresistive sensor;
Setting, by the system controller, a point where the intensity of the detected magnetic signal is rapidly changed in comparison with the reference magnetic signal as a measurement reference position;
Detection method using a magnetoresistive sensor comprising a.
The method of claim 12,
The first step and the second step,
And a magnetoresistive sensor, which is a step of approaching and measuring the magnetoresistive sensor through a transfer module for transporting the specimen fixing unit in an area to which a magnetic field is applied, and storing the specimen in a system controller.
The method according to claim 13,
The reference magnetic signal or the detection magnetic signal of the first step and the second step,
And storing the magnetic signal measured by the magnetoresistive sensor in a system controller via an amplifier and an AD converter.
The method according to claim 13,
The reference magnetic signal or the detection magnetic signal of the first step and the second step,
And storing the magnetic signal measured by the magnetoresistive sensor in a system controller via an amplifying unit, a filter for removing noise, and an AD converter.
The method according to any one of claims 12 to 15,
After step 3,
And detecting the specimen by horizontally scanning the specimen fixing unit at the measurement reference position.
The method according to any one of claims 12 to 15,
After step 3,
4. The method of claim 1, further comprising detecting a sample by horizontally scanning the sample fixing unit at a position partially spaced downward from the measurement reference position.
The method according to any one of claims 12 to 15,
The measurement reference position is a detection method using a magnetoresistive sensor is formed in a range that does not contact the magnetoresistive sensor.

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