KR20120004958A

KR20120004958A - Polymyxin b or e synthetase and gene cluster thereof

Info

Publication number: KR20120004958A
Application number: KR1020110147621A
Authority: KR
Inventors: 박승환; 최수근; 박수영; 김지현; 류충민
Original assignee: 한국생명공학연구원
Priority date: 2011-12-30
Filing date: 2011-12-30
Publication date: 2012-01-13
Also published as: KR101261870B1

Abstract

PURPOSE: A polymyxin B or E biosynthetic enzyme isolated from Paenibacillus sp. strain is provided to develop antibiotics containing derivatives. CONSTITUTION: A polymyxin E biosynthetic enzyme contains PmxA polypeptide small unit having an amino acid sequence of sequence number 4, PmxB polypeptide small unit having an amino acid of sequence number 5, and PmxE polypeptide small unit having an amino acid of sequence number 6. An expression vector contains a nucleotide encoding the polymyxin E biosynthetic enzyme. A transformed host cell is prepared by transducing the expression vector by achromatophilia. The polymyxin E is prepared by culturing the transformed host cell in a nutrient medium.

Description

Polymyxin B or E biosynthetic enzyme and gene group encoding the same {Polymyxin B or E synthetase and gene cluster thereof}

본 발명은 그람 양성 세균인 패니바실러스(Paenibacillus) 속으로부터 분리한 폴리믹신 B 또는 E 생합성 효소 및 이를 코딩하는 유전자 클러스터에 관한 것으로서, 구체적으로는 패니바실러스 폴리믹사 F4(Paenibacillus polymyxa F4) 균주 및 ATCC21830(Paenibacillus polymyxa ATCC21830) 균주로부터 분리된 각각 폴리믹신 B 및 폴리믹신 E를 생합성할 수 있는 폴리믹신 생합성 효소, 이를 코딩하는 유전자 클러스터, 상기 유전자군을 이용한 폴리믹신 B 또는 E 또는 이의 유도체의 제조 방법에 관한 것이다.
The present invention relates to a polymyxin B or E biosynthetic enzyme isolated from the genus Paenibacillus , which is a Gram-positive bacterium, and a gene cluster encoding the same, specifically, Paenibacillus polymyxa F4 (Paenibacillus polymyxa F4) strain and ATCC21830 ( Paenibacillus polymyxa ATCC21830) Polymyxin biosynthetic enzyme capable of biosynthesizing polymyxin B and polymyxin E, respectively, isolated from the strain, a gene cluster encoding the same, and a method of preparing polymyxin B or E or a derivative thereof using the gene group.

비리보솜 펩티드 합성 효소(non-ribosomal peptide synthetase, 이하 'NRPS'라 약칭함)는 NRPS 복합체를 구성하는 하나 이상의 ORF(open reading frame)로 이루어지며, 각 NRPS 또는 NRPS 서브유닛은 하나 이상의 모듈을 포함한다. 모듈은 하나의 빌딩 블록(예, 하나의 아미노산)을 성장하는 펩티드 체인에 연결하는 촉매 단위로 정의된다. NRPS 단백질 주형을 형성하는 생합성 모듈의 순서 및 특이성은 최종적인 펩티드 생성물의 서열과 구조를 지배한다. 따라서, 유전자 코드에 따른 리보솜-매개 RNA 번역과 관련 없이 독립적으로 이루어지는 NRPS 과정은 리보솜에 의해 RNA 주형으로부터 번역되는 펩티드에 비해 방대한 구조적 다양성을 나타내는 펩티드를 생산할 수 있다. 이들은 D- 및 L-형 아미노산과 히드록시산의 연결, 선형, 고리형 또는 분지 고리형 구조를 형성하는 주 펩티드 체인내의 변이 및 산화, 아실화, 글리코실화, N-메틸화 및 헤테로사이클형 고리 형성을 포함한 부가의 구조적 변형을 포함한다.Non-ribosomal peptide synthetase (hereinafter abbreviated as'NRPS') consists of one or more open reading frames (ORFs) constituting the NRPS complex, and each NRPS or NRPS subunit contains one or more modules. do. A module is defined as a catalytic unit that connects one building block (eg, one amino acid) to a growing peptide chain. The order and specificity of the biosynthetic modules forming the NRPS protein template govern the sequence and structure of the final peptide product. Thus, the NRPS process, which is independent of ribosome-mediated RNA translation according to the genetic code, can produce peptides that exhibit vast structural diversity compared to peptides translated from RNA templates by ribosomes. These are the linkage of D- and L-type amino acids with hydroxy acids, mutations and oxidation in the main peptide chain to form a linear, cyclic or branched cyclic structure, acylation, glycosylation, N-methylation and heterocyclic ring formation. Includes additional structural modifications, including.

NRPS중 하나인 폴리믹신 생합성 효소는 폴리믹신을 구성하는 각 아미노산 모노머를 순차적으로 결합시키며, 필요할 경우 아미노산의 변형을 유도하여 전체 아미노산의 체인을 완성하고 링 구조를 만들어 펩티드 항생제를 합성한다. NRPS의 각 모듈은 최소한 A, C, T 도메인으로 구성되어 있는데 A 도메인(adenylation domain)은 아미노산 모노머를 선택하고 활성화시키며, T 도메인(thiolation domain, PCP라고도 불림)은 아미노산 모노머 기질 및 합성중인 펩티드 중간체를 촉매중심점(catalytic center)에 전달해주는 역할을 수행하고, C 도메인(condensation domain)은 펩타이드 본드 형성을 촉매하는 역할을 담당하고 있다. Polymyxin biosynthetic enzyme, one of NRPS, sequentially binds each amino acid monomer constituting polymyxin and, if necessary, induces modification of amino acids to complete a chain of amino acids and form a ring structure to synthesize peptide antibiotics. Each module of NRPS consists of at least A, C, and T domains. A domain (adenylation domain) selects and activates amino acid monomers, and T domain (thiolation domain, also called PCP) is an amino acid monomer substrate and a peptide intermediate being synthesized. It plays a role of delivering to the catalytic center, and the C domain (condensation domain) plays a role of catalyzing the formation of peptide bonds.

최근 그라미시딘(Gramicidin) 생합성 유전자의 페닐알라닌을 인식하는 A 도메인의 3차 구조가 밝혀졌는데 특정 아미노산과 결합하는 부위에 8개의 아미노산 잔기가 관여함이 밝혀졌다(Conti E. et al., EMBO J. 16: 4174-4183, 1997). A 도메인의 아미노산 서열을 기존에 알려진 A 도메인의 아미노산 염기서열과 비교 분석한 결과 같은 아미노산을 인식하는 A 도메인은 상기 8개의 아미노산 잔기가 매우 높은 상동성을 가지고 있음을 확인하였고, 따라서 상기 8개의 아미노산 잔기를 분석하면 특정 A 도메인이 어떤 아미노산을 인식하고 결합하는지 알 수 있게 되었다(Challis G.L. et al., Chem . Biol . 7: 211-224, 2000).Recently, the tertiary structure of the A domain that recognizes phenylalanine in the biosynthesis gene of Gramicidin was revealed, and it was found that 8 amino acid residues are involved in the site that binds to a specific amino acid (Conti E. et al. al ., EMBO J. 16: 4174-4183, 1997). As a result of comparative analysis of the amino acid sequence of the A domain with the previously known amino acid sequence of the A domain, it was confirmed that the A domain recognizing the same amino acid has very high homology, and thus the 8 amino acids Analysis of the residues reveals which amino acids a specific A domain recognizes and binds to (Challis GL et al., Chem . Biol . 7: 211-224, 2000).

이와 같은 핵심 도메인과 더불어 L- 아미노산을 D-아미노산으로 전환시키는 역할을 하는 광학이성질체화 도메인(E domain 또는 epimerization domain)과 TE 도메인(termination domain)등이 있으며, 상기 도메인들은 일반적으로 특이적인 아미노산 모티프 또는 특징에 의해 구별된다.In addition to these core domains, there are optical isomerization domains (E domains or epimerization domains) and TE domains (termination domains) that serve to convert L- amino acids into D-amino acids, and the domains are generally specific amino acid motifs. Or distinguished by features.

유전 공학적 조작 및 생체 내 재조합에 의해 DNA 수준에서 모듈의 수와 순서를 변화시킴으로써 새로운 특이성을 갖는 새로운 효소를 설계하기 위해 모듈 구조를 이용할 수 있다. 예를 들어, 재조합 기술을 이용하여 이종성 NRPS에서 유래한 도메인을 교환하거나(Schneider et al., Mol . Gen . Genet., 257: 308-318, 1998), A 도메인의 기질 결합 포켓을 형성하는 잔기를 변화시켜 모듈 내에 새로운 기질 특이성을 설계하는 방법 등이 가능하다(Cane et al., Chem . Biol . 6: 319-325, 1999).Modular structures can be used to design new enzymes with new specificities by changing the number and order of modules at the DNA level by genetic engineering and in vivo recombination. For example, using recombinant technology to exchange domains derived from heterologous NRPS (Schneider et al ., Mol . Gen. Genet ., 257: 308-318, 1998), a method of designing a new substrate specificity in the module by changing the residues that form the substrate binding pocket of the A domain (Cane et al. al ., Chem . Biol . 6: 319-325, 1999).

폴리믹신은 바실러스 속 혹은 패니바실러스 속으로부터 분리된 항생물질로서, 일반적인 폴리펩티드처럼 유전자에 의해 코딩되어 리보솜에 의해 합성되는 것이 아니라 비리보솜 펩티드 생합성 효소에 의해 합성되는 항생물질이다(Marahiel M.A. et al ., Chem . Rev . 97: 2651-2673, 1997; Doekel S. et al., Metab . Eng. 6: 64-77, 2001). Polymyxin is an antibiotic isolated from the genus Bacillus or the genus Panibacillus, and is an antibiotic that is synthesized by non-ribosomal peptide biosynthetic enzymes, rather than by genes encoded by genes and synthesized by ribosomes like normal polypeptides (Marahiel MA et al. al . , Chem . Rev. 97: 2651-2673, 1997; Doekel S. et al ., Metab . Eng . 6: 64-77, 2001).

폴리믹신은 약 1200 Da(1.2 kDa)의 분자량을 가지고 있다(Storm D.R. et al., Ann Rev . Biochem. 46: 723-763, 1977). 기본구조는 7개의 아미노산으로 구성된 다중양이온성 펩티드 링(polycationic peptide ring)과 3개의 아미노산으로 구성된 펩티드 곁가지로 되어 있다. 따라서 모두 10개의 아미노산으로 구성되어있는데 이중 2,4-다이아미노부티릭산(Dab, 2,4-dimainobutyric acid)이 높은 비율로 포함되어 있다. 펩티드 곁가지의 아미노말단(amino terminus)에 지방산이 결합되어 있는데 이 지방산은 주로 6-메칠옥타노익 산(6-methyloctanoic acid) 또는 6-메칠헵타노익 산(6-methylheptanoic acid)이다(도 3 참조). 이러한 구조는 폴리믹신이 물 또는 유기용매 모두에 잘 녹는 성질을 제공한다.Polymyxin has a molecular weight of about 1200 Da (1.2 kDa) (Storm DR et al., Ann Rev. Biochem . 46: 723-763, 1977). The basic structure consists of a polycationic peptide ring consisting of 7 amino acids and a peptide side branch consisting of 3 amino acids. Therefore, it is composed of all 10 amino acids, of which 2,4-diaminobutyric acid (Dab, 2,4-dimainobutyric acid) is contained in a high proportion. A fatty acid is bound to the amino terminus of the peptide side branch, which is mainly 6-methyloctanoic acid or 6-methylheptanoic acid ( see FIG. 3 ). . This structure provides the property that polymyxin is well soluble in both water and organic solvents.

폴리믹신은 세포막의 투과성을 변화시켜 세포를 사멸시키는 항생물질로서 하기와 같은 작용기전을 나타낸다. Polymyxin is an antibiotic that kills cells by changing the permeability of cell membranes and exhibits the following mechanisms of action.

먼저, 폴리믹신의 다중양이온성 펩티드 링이 세포막의 리포폴리사카라이드 (lipopolysaccharide)를 안정화시키고 있는 칼슘과 마그네슘의 브릿지(bridge)를 치환함으로써 세포에 접촉한 다음, 세포막의 리포폴리사카라이드와 폴리믹신의 지방산 잔기가 반응하여 폴리믹신과 세포막의 결합을 공고히 하게 되어, 최종적으로 폴리믹신이 세포의 외막에 끼어들어 세포막을 파괴하게 된다(Hermsen E.D. et al., Infect. Dis . Clin . N. Am . 17: 545-562, 2003).
First, the polycationic peptide ring of polymyxin contacts the cell by replacing the bridge between calcium and magnesium, which stabilizes the lipopolysaccharide of the cell membrane. The fatty acid residue of the god reacts to solidify the binding between polymyxin and the cell membrane, and finally polymyxin intervenes in the outer membrane of the cell and destroys the cell membrane (Hermsen ED et al ., Infect. Dis . Clin . N. Am . 17: 545-562, 2003).

1947년 패니바실러스 폴리믹사 균으로부터 처음으로 폴리믹신 B가 분리된 이후 최소한 15종의 폴리믹신이 보고되었다(Storm D.R., et al., Annu . Rev . Biochem., 46: 723-763, 1977; Silaev, A. B. et al., Zh. Obshch. Khim. 45: 2331-2337, 1975; Martin N.I. et al., J. Biol . Chem. 278: 13124-13132, 2003). 상기 폴리믹신을 이용한 항생제인 폴리믹신 B 설페이트(polymyxin B sulfate)는 0.01 ㎍/ml의 농도에서 88 %의 슈도모나스 에어로지노사(Pseudomonas aeruginosa)에 대해 치사 효과를 나타내었으나, 폴리믹신 E(colistin)는 0.1 ㎍/ml의 농도에서 치사 효과를 나타내었다. 폴리믹신 B와 E 둘 다 2 ㎍/ml 미만의 농도에서는 대부분의 대장균(Escheridhia coli) 및 슈도모나스 에어로지노사(Pseudomonas aeruginosa)에 효과를 나타내었고, 모든 엔테로박터(Enterobacter), 살모넬라(Salmonella), 쉬겔라(Shigella), 파스테렐라(Pasteurella), 브루셀라(Brucella) 및 보데텔라(Bordetella) 균에도 살균 효과를 나타내었다. 그러나, 프로테우스(Proteus), 세라치아(Serratia), 프로비덴시아(Providencia) 및 에드워드시엘라 (Edwardsiella) 등에는 200 ㎍/ml의 농도 이상에서도 효과가 나타나지 않았다. 또한, 그람 양성 세균, 곰팡이 및 혐기성 세균에도 효과가 없는 것으로 알려져 있다 (Nord N.M. et al., N. Engl . J. Med . 270: 1030-1035, 1964).At least 15 polymyxins have been reported since polymyxin B was first isolated from Panibacillus polymyxa in 1947 (Storm DR, et al ., Annu . Rev. Biochem. , 46: 723-763, 1977; Silaev, AB et al ., Zh. Obshch. Khim. 45: 2331-2337, 1975; Martin NI et al ., J. Biol . Chem . 278: 13124-13132, 2003). Polymyxin B sulfate, an antibiotic using polymyxin, showed a lethal effect against 88% of Pseudomonas aeruginosa at a concentration of 0.01 μg/ml, but polymyxin E (colistin) It showed a lethal effect at a concentration of 0.1 μg/ml. Both polymyxins B and E at concentrations less than 2 μg/ml are used in most Escheridhia coli) and showed the effect on Pseudomonas aero not labor (Pseudomonas aeruginosa), all Enterobacter (Enterobacter), Salmonella (Salmonella), Shh Gela (Shigella), wave Ste Pasteurella (Pasteurella), brucellosis (Brucella) and Bode telra ( Bordetella ) bacteria also showed a bactericidal effect. However, Proteus ( Proteus ), Serratia (Serratia), Providencia ( Providencia ), and Edwardsiella (Edwardsiella) did not show the effect even at a concentration of 200 ㎍ / ml or more. In addition, it is known to have no effect on Gram-positive bacteria, fungi, and anaerobic bacteria (Nord NM et al ., N. Engl . J. Med . 270: 1030-1035, 1964).

상기와 같은 효과에 의해 폴리믹신은 1970년대 초반까지 이들 병원성 미생물에 의한 질병의 치료제로 많이 사용하였으나, 열, 발진 및 통증 등의 부작용을 동반하며 특히 심각한 신장독성과 신경독성을 유발하기 때문에(Hermsen, E. D. et al. Infect . Dis . Clin . North Am ., 17:545-562, 2003; Pedersen M.F. et al., Invest. Urol. 9: 234-237, 1971) 점차 안전성이 높은 다른 항생제에 의해 대체되었고, 그에 따라 사용량이 급격히 줄어들게 되었으며 최근에는 주로 연고제와 같이 국소적인 상처의 치료제로 사용되고 있는 실정이다.Due to the above effects, polymyxin was widely used as a therapeutic agent for diseases caused by these pathogenic microorganisms until the early 1970s, but it has side effects such as fever, rash, and pain, and in particular causes severe kidney toxicity and neurotoxicity (Hermsen , ED et al . Infect . Dis . Clin . North Am . , 17:545-562, 2003; Pedersen MF et al ., Invest. Urol . 9: 234-237, 1971) It was gradually replaced by other antibiotics with high safety, and as a result, the amount of use was rapidly reduced, and recently, it is mainly used as an ointment as a local wound treatment.

오늘날, 많이 사용하고 있는 항생제들에 대해 저항성을 가진 병원균이 빈번하게 출현함에 따라 병원성 그람 음성 세균에 탁월한 살균효과를 보이며 특히 베타 락탐계, 아미노글리코시드계 및 플루오로퀴놀론계 항생제에 저항성을 보이는 슈도모나스 에어로지노사(Pseudomonas aeruginosa) 및 아시네토박터 바우만니 (Acinetobacter baumannii) 등에 탁월한 살균효과를 보이는 폴리믹신이 다시 주목받게 되었다.Today, Pseudomonas exhibits excellent bactericidal effect against pathogenic Gram-negative bacteria as pathogens resistant to antibiotics used frequently appear, and in particular, Pseudomonas, which is resistant to beta-lactam, aminoglycoside, and fluoroquinolone antibiotics. Aerogenosa ( Pseudomonas) aeruginosa ) and Acinetobacter baumannii (Acinetobacter baumannii). Polymyxin, which exhibits excellent bactericidal effects, has attracted attention again.

레빈 등의 보고에 의하면 기존 항생제에 저항성을 가진 슈도모나스 에어로지노사 및 아시네토박터 바우만니에 의해 발병된 60명의 병원감염증 환자들을 대상으로 정맥주사용 콜리스틴(폴리믹신 E)을 처방한 결과 58%의 환자가 치료되었으며 (Levin A.S. et al. Clin . Infect . Dis . 28: 1008-1011, 1999), 스타인 등은 모든 항생제에 저항성을 가진 슈도모나스 에어로지노사에 의해 발병된 3명의 골수염 환자를 콜리스틴을 이용하여 치료하였다는 보고가 있으며(Stein A. et al., Clin . Infect. Dis . 35: 901-902, 2002), 다른 그룹에서는 항생제 저항성 아시네토박터에 의한 뇌막염을 성공적으로 치료하였다고 보고하였다(Jimenez-Mejias M.E. et al., Eur. J. Clin . Microbiol . Infect . Dis . 21: 212-214, 2002). 또한 폴리믹신 B를 사용하여 항생제 저항성 클렙시엘라 뉴모니아에 의한 뇌실염(ventriculis)을 치료하였다는 보고도 있다(Segal-Maurer S. et al., Clin . Infect . Dis . 28: 1134-1138, 1999).
According to a report by Levin et al., 58% of patients with intravenous colistin (polymyxin E) were prescribed for 60 patients with hospital infections caused by Pseudomonas Aerogenosa and Acinetobacter Baumann who were resistant to conventional antibiotics. Of patients were treated (Levin AS et al . Clin . Infect . Dis . 28: 1008-1011, 1999), Stein et al. reported that three patients with osteomyelitis caused by Pseudomonas aerogenosa resistant to all antibiotics were treated with colistin (Stein A. et al.) al ., Clin . Infect. Dis . 35: 901-902, 2002), another group reported a successful treatment of meningitis caused by antibiotic-resistant acinetobacter (Jimenez-Mejias ME et al ., Eur. J. Clin . Microbiol . Infect . Dis . 21: 212-214, 2002). In addition, there is a report that polymyxin B was used to treat antibiotic-resistant Klebsiella pneumoniae-induced ventritis (Segal-Maurer S. et al.) al ., Clin . Infect . Dis . 28: 1134-1138, 1999).

상술한 바에 따라 최근 폴리믹신이 기존에 알려진 항생제에 저항성을 가진 그람음성 세균의 치료제로 다시 주목받게 됨에 따라 그 수요가 늘어날 것으로 예상된다. As described above, the demand for polymyxin is expected to increase as polymyxin has recently attracted attention again as a therapeutic agent for Gram-negative bacteria resistant to previously known antibiotics.

항생제 생산량 증대의 한 방법으로서 항생제 생합성 유전자를 산업적 대량배양이 용이한 균주에 도입한 예들이 보고되었으며(Eppelmann K. et al., J. Biol . Chem. 276: 34824-34831, 2001; Pfeifer B.A. et al., Microbiol . Mol . Biol . Rev. 65: 106-118, 2001) 항생제 생합성 유전자의 프로모터를 강한 것으로 치환함으로써 생산성 증대를 이룬 보고도 있었다(Tsuge K. et al., J. Bacteriol. 183: 6265-6273, 2001). 또한, 항생제 생합성 유전자의 모듈 또는 도메인의 재구성을 통하거나(Mootz H.D. et al., Proc . Natl . Acad . Sci . USA 97: 5848-5853, 2000; Ferra F.D. et al., J. Biol . Chem. 272: 25304-25309, 1997) 도메인 내의 특정 아미노산을 치환하는 방법으로(Eppelmann K. et al., Biochemistry 41: 9718-9726, 2002) 새로운 항생제의 개발도 시도되고 있다. 폴리믹신 생합성 유전자의 경우에는 분리 동정이 어려워 최근까지도 클로닝이 되지 않았으며, 본 발명자들에 의해 최초로 폴리믹신 A(또는 M)을 생합성할 수 있는 폴리믹신 생합성 유전자 클러스트가 클로닝되었고(대한민국 특허 제750658호), 상기 유전자 클러스트를 폴리믹신을 생산하지 않는 Bacillus subtilis 균주의 게놈에 도입할 경우, 폴리믹신 A(또는 M)이 생성됨을 입증하였다(Choi et al., J. Bacteriol . 191(10): 3350-3358, 2009). As a method of increasing the production of antibiotics, examples of introducing antibiotic biosynthetic genes into strains that are easy to mass-culture industrially have been reported (Eppelmann K. et al. al ., J. Biol . Chem . 276: 34824-34831, 2001; Pfeifer BA et al ., Microbiol . Mol . Biol . Rev. 65: 106-118, 2001) There was also a report that productivity was improved by replacing the promoter of the antibiotic biosynthetic gene with a strong one (Tsuge K. et al. al ., J. Bacteriol . 183: 6265-6273, 2001). In addition, through reconfiguration of the module or domain of the antibiotic biosynthetic gene (Mootz HD et al ., Proc . Natl . Acad . Sci . USA 97: 5848-5853, 2000; Ferra FD et al ., J. Biol . Chem . 272: 25304-25309, 1997) by substituting a specific amino acid in the domain (Eppelmann K. et al) al ., Biochemistry 41: 9718-9726, 2002) The development of new antibiotics is also being attempted. In the case of polymyxin biosynthetic genes, it was difficult to isolate and identify, and thus, cloning was not possible until recently, and the first polymyxin biosynthetic gene cluster capable of biosynthesizing polymyxin A (or M) was cloned by the present inventors (Korean Patent No. 750658 Ho), Bacillus that does not produce polymyxin in the gene cluster subtilis strain, when introduced into the genome, it was demonstrated that polymyxin A (or M) was produced (Choi et al ., J. Bacteriol . 191(10): 3350-3358, 2009).

그러나, 상기 폴리믹신 생합성 유전자에 의해 생산이 되는 폴리믹신은 폴리믹신 A(또는 M)으로서, 실제 FDA 승인을 받은 항생제인 폴리믹신 B 또는 E와는 달리 임상시험 절차를 거친 신약 승인절차를 거쳐야 하기 때문에, 상업적 이용가능성이 떨어지는 단점이 있다. 따라서, 산업상 이용가능성이 큰 폴리믹신 B 또는 E를 생합성할 수 있는 폴리믹신 생합성 유전자를 클로닝하는 것이 본 기술분야에서의 시급한 해결과제이다. 그러나, 상기 폴리믹신 A(또는 M) 생합성 유전자의 확보에도 불구하고, 폴리믹신 생합성 유전자의 경우 그 크기가 매우 크고, 상동성이 매우 높은 소단위 또는 모듈이 지속적으로 반복되는 구조를 가지고 있기 때문에, 다른 종류의 폴리믹신 생합성 유전자의 경우, 제대로 폴리믹신을 생합성할 수 있는 수준의 유전자 클러스터를 온전히 확보하는 것은 매우 어려운 일로 평가된다.
However, since polymyxin produced by the polymyxin biosynthetic gene is polymyxin A (or M), unlike polymyxin B or E, which are actually FDA-approved antibiotics, it must undergo a new drug approval procedure that has undergone clinical trials. However, there is a drawback of poor commercial availability. Therefore, cloning a polymyxin biosynthetic gene capable of biosynthesizing polymyxin B or E, which has high industrial applicability, is an urgent problem in the art. However, despite the securing of the polymyxin A (or M) biosynthetic gene, the polymyxin biosynthetic gene has a structure in which the size of the polymyxin biosynthetic gene is very large and has a very high homology subunit or module. In the case of a kind of polymyxin biosynthetic gene, it is considered to be very difficult to fully secure a gene cluster at a level that can properly biosynthesize polymyxin.

이에, 본 발명자들은 폴리믹신 B 또는 E의 생합성 유전자를 분리 동정하기 위해 예의 노력한 결과, 신규 패니바실러스 폴리믹사 균주인 Paenibacillus polymyxa F4 균주(수탁번호: KCTC11667BP) 및 Paenibacillus polymyxa ATCC23830 균주로부터 각각 폴리믹신 B 및 E를 생합성할 수 있는 폴리믹신 생합성 유전자를 분리동정하는데 성공하였고, 상기 균주들이 폴리믹신 B 및 E를 각각 생산할 수 있음을 입증함으로써, 본 발명을 완성하였다.
Accordingly, the present inventors made diligent efforts to isolate and identify the biosynthetic gene of polymyxin B or E, as a result of the novel Panibacillus polymyxa strain, Paenibacillus polymyxa F4 strain (accession number: KCTC11667BP) and Paenibacillus. ATCC23830 strain was from polymyxa successfully identify each polymyxin B and polymyxin remove the biosynthetic genes that can be the E biosynthesis, by proving that the strains are capable of producing polymyxin B and E, respectively, and completed the present invention.

대한민국 특허 제750658호Korean Patent No. 750658

Ansari et al., Nucleic acids Res. 32: W405-W413, 2004Ansari et al., Nucleic acids Res. 32: W405-W413, 2004 Cane et al., Chem. Biol. 6: 319-325, 1999Cane et al., Chem. Biol. 6: 319-325, 1999 Challis G.L. et al., Chem. Biol. 7: 211-224, 2000Challis G.L. et al., Chem. Biol. 7: 211-224, 2000 Choi et al., J. Bacteriol. 191(10): 3350-3358, 2009Choi et al., J. Bacteriol. 191(10): 3350-3358, 2009 Conti E. et al., EMBO J. 16: 4174-4183, 1997Conti E. et al., EMBO J. 16: 4174-4183, 1997 Doekel S. et al., Metab. Eng. 6: 64-77, 2001Doekel S. et al., Metab. Eng. 6: 64-77, 2001 Eppelmann K. et al., Biochemistry 41: 9718-9726, 2002Eppelmann K. et al., Biochemistry 41: 9718-9726, 2002 Eppelmann K. et al., J. Biol. Chem. 276: 34824-34831, 2001Eppelmann K. et al., J. Biol. Chem. 276: 34824-34831, 2001 Ferra F.D. et al., J. Biol. Chem. 272: 25304-25309, 1997Ferra F.D. et al., J. Biol. Chem. 272: 25304-25309, 1997 Hermsen E.D. et al., Infect. Dis. Clin. N. Am. 17: 545-562, 2003Hermsen E.D. et al., Infect. Dis. Clin. N. Am. 17: 545-562, 2003 Jimenez-Mejias et al., Eur. J. Clin. Microbiol. Infect. Dis. 21: 212-214, 2002Jimenez-Mejias et al., Eur. J. Clin. Microbiol. Infect. Dis. 21: 212-214, 2002 Koyama, Y. et al., J. Antibiot., 3: 457-458, 1950Koyama, Y. et al., J. Antibiot., 3: 457-458, 1950 Levin A.S. et al. Clin. Infect. Dis. 28: 1008-1011, 1999Levin A.S. et al. Clin. Infect. Dis. 28: 1008-1011, 1999 Marahiel M.A. et al., Chem. Rev. 97: 2651-2673, 1997Marahiel M.A. et al., Chem. Rev. 97: 2651-2673, 1997 Martin N.I. et al., J. Biol. Chem. 278: 13124-13132, 2003Martin N.I. et al., J. Biol. Chem. 278: 13124-13132, 2003 Mootz H.D. et al., Proc. Natl. Acad. Sci. USA 97: 5848-5853, 2000Mootz H.D. et al., Proc. Natl. Acad. Sci. USA 97: 5848-5853, 2000 Nord N.M. et al., N. Engl. J. Med. 270: 1030-1035, 1964Nord N.M. et al., N. Engl. J. Med. 270: 1030-1035, 1964 Pedersen M.F. et al., Invest. Urol. 9: 234-237, 1971Pedersen M.F. et al., Invest. Urol. 9: 234-237, 1971 Pfeifer B.A. et al., Microbiol. Mol. Biol. Rev. 65: 106-118, 2001Pfeifer B.A. et al., Microbiol. Mol. Biol. Rev. 65: 106-118, 2001 Schneider et al., Mol. Gen. Genet., 257: 308-318, 1998Schneider et al., Mol. Gen. Genet., 257: 308-318, 1998 Segal-Maurer S. et al., Clin. Infect. Dis. 28: 1134-1138, 1999Segal-Maurer S. et al., Clin. Infect. Dis. 28: 1134-1138, 1999 Silaev, A. B. et al., Zh. Obshch. Khim. 45: 2331-2337, 1975Silaev, A. B. et al., Zh. Obshch. Khim. 45: 2331-2337, 1975 Stein A. et al., Clin. Infect. Dis. 35: 901-902, 2002Stein A. et al., Clin. Infect. Dis. 35: 901-902, 2002 Storm D.R. et al., Ann. Rev. Biochem. 46: 723-763, 1977Storm D.R. et al., Ann. Rev. Biochem. 46: 723-763, 1977 Tsuge K. et al., J. Bacteriol. 183: 6265-6273, 2001Tsuge K. et al., J. Bacteriol. 183: 6265-6273, 2001

본 발명의 목적은 폴리믹신 B 또는 E를 생합성할 수 있는 폴리믹신 생합성 효소를 제공하는 것이다.An object of the present invention is to provide a polymyxin biosynthetic enzyme capable of biosynthesizing polymyxin B or E.

본 발명의 또다른 목적은 상기 폴리믹신 생합성 효소를 암호화하는 폴리뉴클레오티드를 제공하는 것이다.Another object of the present invention is to provide a polynucleotide encoding the polymyxin biosynthetic enzyme.

본 발명의 다른 목적은 상기 폴리뉴클레오티드의 전부 또는 일부를 포함하는 발현벡터를 제공하는 것이다.Another object of the present invention is to provide an expression vector containing all or part of the polynucleotide.

본 발명의 다른 목적은 상기 발현벡터가 비염색체성으로 형질도입되거나 상기 폴리뉴클레오티드가 게놈 내에 삽입되어 제조된 형질전환 숙주세포를 제공하는 것이다.Another object of the present invention is to provide a transformed host cell prepared by transducing the expression vector non-chromosomally or inserting the polynucleotide into a genome.

본 발명의 또 다른 목적은 상기 형질전환 숙주세포를 이용한 폴리믹신 B 또는 E의 제조방법에 관한 것이다.
Another object of the present invention relates to a method for preparing polymyxin B or E using the transformed host cell.

1. 용어의 정의1. Definition of terms

이하 본 발명에서 사용하는 용어를 설명한다:The terms used in the present invention will be described below:

비- 리보좀 펩티드 합성효소( NRPS , non - ribosomal peptide synthetase ): 펩티드 합성이 mRNA-tRNA 및 리보좀에 의한 번역과정을 거치지 않고 단위 아미노산을 부가하는 하나 또는 그 이상의 개방판독틀(ORF)에 의해 번역된 효소 또는 효소 소단위체의 복합체를 의미한다. 각 NRPS 또는 NPRS 소단위체는 하나 이상의 모듈을 포함한다. Non- ribosomal peptide synthase ( NRPS , non - ribosomal peptide synthetase ) : refers to a complex of enzymes or enzyme subunits translated by one or more open reading frames (ORFs) in which peptide synthesis does not undergo translation by mRNA-tRNA and ribosomes and adds unit amino acids. Each NRPS or NPRS subunit contains one or more modules.

모듈( module ): 하나의 구조 단위(예를 들어, 아미노산)을 신장하는 펩티드 사슬에 부가하는 기능을 수행하는 단위를 의미한다. 각 모듈은 서로 다른 도메인들로 구성된다. Module (module): means a unit that performs the functions to be added to the peptide chain extending to one of the structural unit (e. G., Amino acids). Each module consists of different domains.

도메인( domain ): 상기 모듈 내에서 사슬 신장의 계속을 위한 일련의 반응을 관장하는 단위로서, A(adenylation) 도메인, C(condensation) 도메인, T(thiolation) 도메인, E(epimerization) 도메인, TE(termination) 도메인 등으로 구성되는데, A 도메인은 아미노산 단량체를 선택하고 활성화하는 역할을 수행하고, C 도메인은 펩티드 결합을 매개하는 역할을 수행하며, 펩티딜기 운반 단백질(peptidyl carrier protein, PCP)로도 불리우는 T 도메인은 기질의 이동 및 중간체를 반응 센터로 신장시키는 역할을 수행하고, E 도메인은 L-형 아미노산을 D-형 아미노산으로 변환시키는 역할을 수행하며, TE 도메인은 아미노산의 부가반응을 종결시키는 역할을 수행한다. Domain (domain): a unit for managing the series of reactions for the continuation of the chain extender in said module, A (adenylation) domain, C (condensation) domain, T (thiolation) domain, E (epimerization) domain, TE ( termination) domain, etc., where the A domain plays a role of selecting and activating amino acid monomers, the C domain playing a role of mediating peptide bonds, and T, also called a peptidyl carrier protein (PCP). The domain plays the role of transferring the substrate and extending the intermediate to the reaction center, the E domain plays the role of converting the L-type amino acid into the D-type amino acid, and the TE domain plays the role of terminating the addition reaction of the amino acid. Carry out.

폴리믹신 ( polymyxin ): 바실러스속 또는 패니바실러스속 균으로부터 리보좀에 의해 합성되지 않고, NRPS에 의해 합성되는 항생제를 의미한다. 현재까지, 폴리믹신 A, B, E 및 M 등 총 15가지 이상 알려져 있다. Polymyxin (polymyxin): is not synthesized by the ribosome from Bacillus or Bacillus spp Companion, it refers to antibiotics that are synthesized by the NRPS. To date, polymyxins A, B, E, and M, and more than 15 in total are known.

폴리믹신 합성효소( polymyxin synthetase ): 각 아미노산 단량체를 단계적으로 결합시키고 상기 아미노산을 전체 아미노산 사슬을 완성하도록 변형시키고 최종적으로 펩티드 항생제를 형성하도록 환구조를 형성하는 NRPS의 일종을 의미한다. 본 발명자들은 상기 폴리믹신 합성효소가 PmxA, PmxB 및 PmxE로 각각 독립적으로 번역되어 복합체를 형성함을 최초로 규명한 바 있다(대한민국 특허 제750658호). Polymyxin synthase ( polymyxin synthetase ) : It refers to a kind of NRPS that combines each amino acid monomer in stages, transforms the amino acid to complete the entire amino acid chain, and finally forms a ring structure to form a peptide antibiotic. The present inventors have first identified that the polymyxin synthase is independently translated into PmxA, PmxB, and PmxE to form a complex (Korean Patent No. 750658).

PmxA 폴리펩티드 소단위체 ( PmxA polypeptide subunit ): 폴리믹신 합성효소의 소단위체중 하나이다. 상기 PmxA 폴리펩티드 소단위체는 폴리믹신의 여섯번째 내지 아홉번째 아미노산을 각각 부가하는 네 개의 모듈을 포함한다. PmxA polypeptide subunit ( PmxA polypeptide subunit ) : One of the subunits of polymyxin synthase. The PmxA polypeptide subunit includes four modules each adding the sixth to ninth amino acids of polymyxin.

PmxB 폴리펩티드 소단위체 ( PmxB polypeptide subunit ): 폴리믹신 합성효소의 소단위체 중 하나이다. 상기 PmxB 폴리펩티드 소단위체는 마지막 아미노산인 L-쓰레오닌(L-Thr)을 부가하는 모듈을 포함한다. 상기 PmxB 폴리펩티드 소단위체는 TE 도메인을 가지고 있기 때문에, 상기 TE 도메인에 의해 상기 마지막 아미노산의 카르복실기가 네번째 아미노산인 L-Dab의 두번째 아민에 연결된다. PmxB polypeptide subunit ( PmxB polypeptide subunit ) : One of the subunits of polymyxin synthase. The PmxB polypeptide subunit contains a module that adds the last amino acid, L-threonine (L-Thr). Since the PmxB polypeptide subunit has a TE domain, the carboxyl group of the last amino acid is linked to the second amine of L-Dab, which is the fourth amino acid by the TE domain.

PmxE 폴리펩티드 소단위체 ( PmxA polypeptide subunit ): 폴리믹신 합성효소의 소단위체 중 하나이다. 상기 PmxE 폴리펩티드 소단위체는 N-말단의 다섯 아미노산을 부가하는 다섯 개의 모듈을 가지고 있다. 상기 다섯 아미노산은 N-말단부터 L-Dab, L-Thr, L-Dab (또는D-Dab), L-Dab 및 L-Dab이다. PmxE polypeptide subunit ( PmxA polypeptide subunit ): One of the subunits of polymyxin synthase. The PmxE polypeptide subunit has five modules that add five amino acids at the N-terminus. The five amino acids are L-Dab, L-Thr, L-Dab (or D-Dab), L-Dab and L-Dab from the N-terminus.

유전자 클러스터( gene cluster ): 유전자 클러스터는 단독 ORF는 아니므로 하나의 전사체로부터 다수의 단백질이 번역되는 오페론과 구분되나, 특정 대사작용에 관련된 유전자들이 게놈 내에 연속적으로 존재하는 일종의 유전자 군을 의미한다. 본 발명의 폴리믹신 생합성 효소는 상기 유전자 클러스터 내에 존재하는 세 가지 유전자인 pmxA, pmxB 및 pmxE의 번역산물의 복합체이다. 폴리믹신 생합성 유전자 클러스터 내에는 폴리믹신의 생합성에는 직접 관여하지는 않으나, 폴리믹신의 분비에 관여할 것으로 추정되는 pmxC 및 pmxD도 존재한다.
Gene cluster (gene cluster ) : Since a gene cluster is not a single ORF, it is distinguished from an operon in which a number of proteins are translated from one transcript, but refers to a group of genes in which genes related to a specific metabolism are continuously present in the genome. The polymyxin biosynthetic enzyme of the present invention is a complex of translation products of three genes, pmxA , pmxB, and pmxE, present in the gene cluster. In the polymyxin biosynthesis gene cluster, although not directly involved in the biosynthesis of polymyxin , there are also pmxC and pmxD, which are estimated to be involved in the secretion of polymyxin.

2. 발명의 요약2. Summary of the invention

상기 목적을 달성하기 위해, 본 발명은In order to achieve the above object, the present invention

서열번호 1로 기재되는 아미노산 서열을 갖는 PmxA 폴리펩티드 소단위체, 서열번호 2로 기재되는 아미노산 서열을 갖는 PmxB 폴리펩티드 소단위체 및 서열번호 3으로 기재되는 아미노산 서열을 갖는 PmxE 폴리펩티드 소단위체로 구성되는 분리된 폴리믹신 B 생합성 효소를 제공한다.An isolated polymyxin consisting of a PmxA polypeptide subunit having an amino acid sequence set forth in SEQ ID NO: 1, a PmxB polypeptide subunit having an amino acid sequence set forth in SEQ ID NO: 2, and a PmxE polypeptide subunit having an amino acid sequence set forth in SEQ ID NO: 3 B biosynthetic enzymes are provided.

또한, 본 발명은 서열번호 4로 기재되는 아미노산 서열을 갖는 PmxA 폴리펩티드 소단위체, 서열번호 5로 기재되는 아미노산 서열을 갖는 PmxB 폴리펩티드 소단위체 및 서열번호 6으로 기재되는 아미노산 서열을 갖는 PmxE 폴리펩티드 소단위체로 구성되는 분리된 폴리믹신 E 생합성 효소를 제공한다.In addition, the present invention is composed of a PmxA polypeptide subunit having an amino acid sequence shown in SEQ ID NO: 4, a PmxB polypeptide subunit having an amino acid sequence shown in SEQ ID NO: 5, and a PmxE polypeptide subunit having an amino acid sequence shown in SEQ ID NO: 6. An isolated polymyxin E biosynthetic enzyme is provided.

다른 실시태양에서 본 발명은 상기 폴리믹신 B 생합성 효소를 암호화하는 폴리뉴클레오티드를 제공한다. 이때, 상기 폴리뉴클레오티드는 서열번호 7로 기재되는 핵산서열을 갖는 pmxA 유전자, 서열번호 8로 기재되는 핵산서열을 갖는 pmxB 유전자 및 서열번호 9로 기재되는 핵산서열을 갖는 pmxE 유전자를 포함하는 것이 바람직하나, 이에 제한되는 것은 아니다. 또한, 상기 각각의 유전자들은 하나의 유전자 클러스터 형태로 제공되거나, 서로 구분되는 별개의 벡터에 클로닝되어 제공될 수 있다. 유전자 클러스터의 형태로 제공되는 경우에는 서열번호 10으로 기재되는 핵산서열을 갖는 pmxC 유전자 및 서열번호 11로 기재되는 핵산서열을 갖는 pmxD 유전자를 추가적으로 포함하는 것이 바람직하나, 상기 pmxC 및 pmxD는 폴리믹신 생합성에 직접 관여하지 않고 단지 폴리믹신의 분비에 관련하는 것으로 추정되므로, 이들 유전자의 중요도는 상대적으로 덜한 것으로 판단된다. In another embodiment, the present invention provides a polynucleotide encoding the polymyxin B biosynthetic enzyme. In this case, the polynucleotide may include the pmxE gene having the nucleotide sequence represented by pmxB gene and SEQ ID NO: 9 having a nucleotide sequence represented by pmxA gene, SEQ ID NO: 8 with a nucleic acid sequence represented by SEQ ID NO: 7 one , But is not limited thereto. In addition, each of the genes may be provided in the form of one gene cluster, or may be provided by being cloned into separate vectors that are distinguished from each other. When provided in the form of a gene cluster, it is preferable to additionally include a pmxC gene having a nucleic acid sequence shown in SEQ ID NO: 10 and a pmxD gene having a nucleic acid sequence shown in SEQ ID NO: 11 , but the pmxC and pmxD are polymyxin biosynthesis. Since it is presumed to be related only to the secretion of polymyxin without being directly involved in, the importance of these genes is judged to be relatively less important.

또 다른 실시태양에서, 본 발명은 상기 폴리믹신 E 생합성 효소를 암호화하는 폴리뉴클레오티드를 제공한다. 이때, 상기 폴리뉴클레오티드는 서열번호 12로 기재되는 핵산서열을 갖는 pmxA 유전자, 서열번호 13으로 기재되는 핵산서열을 갖는 pmxB 유전자 및 서열번호 14로 기재되는 핵산서열을 갖는 pmxE 유전자를 포함하는 것이 바람직하나, 이에 제한되는 것은 아니다. 또한, 상기 각각의 유전자들은 하나의 유전자 클러스터 형태로 제공되거나, 서로 구분되는 별개의 벡터에 클로닝되어 제공될 수 있다. 유전자 클러스터의 형태로 제공되는 경우에는 서열번호 15으로 기재되는 핵산서열을 갖는 pmxC 유전자 및 서열번호 16로 기재되는 핵산서열을 갖는 pmxD 유전자를 추가적으로 포함하는 것이 바람직하나, 상기 pmxC 및 pmxD는 폴리믹신 생합성에 직접 관여하지 않고 단지 폴리믹신의 분비에 관련하는 것으로 추정되므로, 이들 유전자의 중요도는 상대적으로 덜한 것으로 판단된다. In another embodiment, the present invention provides a polynucleotide encoding the polymyxin E biosynthetic enzyme. In this case, the polynucleotide may include the pmxE gene having the nucleotide sequence represented by pmxB gene and SEQ ID NO: 14 has a nucleic acid sequence described in pmxA gene, SEQ ID NO: 13 has a nucleic acid sequence represented by SEQ ID NO: 12 one , But is not limited thereto. In addition, each of the genes may be provided in the form of one gene cluster, or may be provided by being cloned into separate vectors that are distinguished from each other. When provided in the form of a gene cluster pmxD with a nucleic acid sequence represented by SEQ ID NO: 16 pmxC gene and having the nucleic acid sequence described in SEQ ID NO: 15 It is preferable to additionally include genes, but the pmxC and pmxD are not directly involved in polymyxin biosynthesis, but are assumed to be only related to the secretion of polymyxin, so the importance of these genes is judged to be relatively less important.

본 발명은 아울러, 상기 폴리믹신 B 또는 E 생합성 효소를 암호화하는 폴리뉴클레오티드의 전부 또는 일부를 포함하는 발현벡터를 제공한다. 상기 폴리뉴클레오티드의 일부는 pmxA, pmxB 및 pmxE의 각각 유전자의 암호화 영역(coding region) 전체를 포함하는 폴리뉴클레오티드 단편을 의미한다.In addition, the present invention provides an expression vector comprising all or part of a polynucleotide encoding the polymyxin B or E biosynthetic enzyme. Part of the polynucleotide refers to a polynucleotide fragment including the entire coding region of each gene of pmxA , pmxB, and pmxE.

상기 발현벡터는 원핵세포 또는 진핵세포에서 외래단백질을 발현시키기 위해 사용되는 것이라면 어떤 것이라도 사용가능하다. 원핵세포용 발현벡터가 바람직하며, 진핵세포용 발현벡터의 경우 효모용, 곤충용 또는 포유동물 세포용 발현벡터의 사용의 사용이 가능하나 효모용 발현벡터가 더욱 바람직하다.The expression vector can be used as long as it is used to express foreign proteins in prokaryotic or eukaryotic cells. Expression vectors for prokaryotic cells are preferred. In the case of expression vectors for eukaryotic cells, expression vectors for yeast, insect, or mammalian cells can be used, but expression vectors for yeast are more preferred.

상업적으로 이용가능한 원핵세포용 발현벡터로는 pET 벡터(Novagen, Inc., USA), pQE 벡터(Qiagen, USA) 및 pGEX(Pharmacia Biotech Inc., USA) 등이 존재하나 이에 제한되는 것은 아니고, 상업적으로 이용가능한 진핵세포용 발현벡터로는 pCI Neo(Promega, USA), pMAMneo(Clontech, USA), pcDNA3(InVitrogen, USA), pMClneo(Stratagene, USA), pXT1(Stratagene, USA), pSG5(Stratagene, USA), EBO-pSV2-neo(ATCC 37593), pBPV-1(8-2)(ATCC 37110), pdBPV-MMTneo (342-12)(ATCC 37224), pRSVgpt(ATCC 37199), pRSVneo(ATCC 37198), pSV2-dhfr(ATCC 37146), pUCTag(ATCC 37460) 및 lZD35(ATCC 37565) 등을 사용할 수 있으나, 이에 제한되는 것은 아니다.Commercially available expression vectors for prokaryotic cells include pET vectors (Novagen, Inc., USA), pQE vectors (Qiagen, USA), and pGEX (Pharmacia Biotech Inc., USA), but are not limited thereto, and are commercially available. Expression vectors for eukaryotic cells that can be used include pCI Neo (Promega, USA), pMAMneo (Clontech, USA), pcDNA3 (InVitrogen, USA), pMClneo (Stratagene, USA), pXT1 (Stratagene, USA), pSG5 (Stratagene, USA). USA), EBO-pSV2-neo (ATCC 37593), pBPV-1 (8-2) (ATCC 37110), pdBPV-MMTneo (342-12) (ATCC 37224), pRSVgpt (ATCC 37199), pRSVneo (ATCC 37198) , pSV2-dhfr (ATCC 37146), pUCTag (ATCC 37460), and lZD35 (ATCC 37565) may be used, but are not limited thereto.

일 실시태양으로 본 발명은 상기 발현벡터가 숙주세포에 비염색체성으로(episomally) 형질도입되거나 상기 폴리뉴클레오티드가 숙주세포의 염색체 내로 삽입되어 제조된 형질전환 숙주세포를 제공한다.In one embodiment, the present invention provides a transformed host cell prepared by transducing the expression vector episomally into a host cell or inserting the polynucleotide into the chromosome of the host cell.

상기 숙주세포는 원핵세포 또는 진핵세포인 것이 바람직하며, 더 바람직하게는 원핵세포이나, 이에 제한되는 것은 아니다.
The host cell is preferably a prokaryotic cell or a eukaryotic cell, more preferably a prokaryotic cell, but is not limited thereto.

상기 원핵세포는 그람양성균, 그람음성균을 포함하는 진성 세균과 고세균을 모두 포함하며 그람양성균이 더 바람직하다. 상기 그람양성균으로는 바실러스속 세균, 포도상구균, 연쇄상구균, 폐렴균, 나병균, 디프테리아균, 파상풍균, 탄저균,방선균 등이 포함되나 이에 제한되는 것은 아니고, 바실러스속 세균 또는 방선균이 더 바람직하다. 상기 그람음성균은 살모넬라균, 이질균, 티푸스균, 대장균, 콜레라균, 페스트균, 임균, 수막염균, 스피로헤타 등이 포함되며, 대장균이 더 바람직하나 이에 제한되는 것은 아니다.The prokaryotic cells include both gram-positive bacteria and gram-negative bacteria, including true bacteria and archaeal bacteria, and gram-positive bacteria are more preferable. The gram-positive bacteria include, but are not limited to, Bacillus bacteria, staphylococcus, streptococcus, pneumococcus, leprosy, diphtheria, tetanus, anthrax, actinomycetes, etc., and Bacillus bacteria or actinomycetes are more preferred. The Gram-negative bacteria include Salmonella, Shigella, Typhoid, E. coli, cholera, plague, gonococcus, meningitis, spirochetta, and the like, and E. coli is more preferable, but is not limited thereto.

또 다른 일실시태양으로 본 발명은 상기 형질전환 숙주세포를 영양배지 내에서 접종하여 배양하는 단계를 포함하는 폴리믹신 B 또는 E를 생산하는 방법을 제공한다. 상기 영양배지는 해당 숙주세포의 배양에 적합한 것이라면 어느 것이라도 사용하는 것이 가능하며, 특별히 이에 제한되는 것이다. 상기 숙주세포가 Dab을 생산하지 못하는 세포주일 경우에는 상기 영양배지 내에 Dab을 추가시킬 수 있다.In another embodiment, the present invention provides a method for producing polymyxin B or E comprising the step of inoculating and culturing the transformed host cells in a nutrient medium. The nutrient medium may be used as long as it is suitable for culturing the host cell, and is particularly limited thereto. If the host cell is a cell line that cannot produce Dab, Dab may be added to the nutrient medium.

본 발명자들은 국내 토양시료에서 포자를 형성하며 대장균과 식물병원성 곰팡이인 Rhizoctonia solani 및 Fusarium oxysporom에 대해 우수한 길항력을 나타내는 균주를 탐색하여 이를 동정한 결과 Paenibacillus polymyxa종의 신규 균주인 것을 규명하였고, 상기 균주가 폴리믹신을 생산하는지 LC/MS 분석을 수행한 결과, 폴리믹신 B를 생산하는 것을 확인하였다(도 1 참조). 이에 따라, 본 발명자들은 상기 신규 균주를 Paebacillus polymyxa F4 균주(이하, 'F4 균주'로 약칭함)로 명명하고, 한국생명공학연구원 내 생물자원센터의 유전자은행에 2010년 3월 16일자로 기탁하여 수탁번호 KCTC11667BP를 부여받았다.The present inventors form spores in domestic soil samples, and E. coli and Rhizoctonia, a phytopathogenic fungus. solani and Fusarium Paenibacillus was identified as a result of searching for and identifying a strain that exhibits excellent antagonism against oxysporom. It was found that it is a new strain of polymyxa species, and as a result of performing LC/MS analysis to see if the strain produced polymyxin, it was confirmed that polymyxa produced B (see FIG. 1). Accordingly, the inventors of the new strain Paebacillus It was named polymyxa F4 strain (hereinafter, abbreviated as'F4 strain'), and was deposited with the gene bank of the Biological Resource Center in the Korea Research Institute of Bioscience and Biotechnology on March 16, 2010, and was given the accession number KCTC11667BP.

상기 F4 균주와 농촌진흥청 농업유전자원센터로부터 구매한 폴리믹신 E(colistin)을 이미 생산하는 것으로 보고된 Paenibacillus polymyxa ATCC21830 균주(이하, 'ATCC21830 균주'로 약칭함)로부터 폴리믹신 생합성 유전자 클러스터를 클로닝하는데 성공하였다. Paenibacillus reported to already produce the F4 strain and polymyxin E (colistin) purchased from the Agricultural Genetic Resource Center of the Rural Development Administration polymyxa ATCC21830 polymyxin succeeded in cloning of the biosynthetic gene cluster from the strain (hereinafter abbreviated as "ATCC21830 strain").

상기 폴리믹신 B 및 E 생합성 유전자 클러스터는 종래에 본 발명자들이 규명한 Paenibacillus polymyxa E681 균주 유래의 폴리믹신 생합성 유전자와 유사하게 pmxA, pmxB, pmxC, pmxD 및 pmxE의 순서로 구성되어 있었으며(도 2 참조), 상기 유전자에 의해 암호화되는 폴리펩티드의 아미노산 서열 분석 결과, 예측된 폴리믹신의 구조는 폴리믹신 B와 E임을 알 수 있었다(도 3 참조). 그러나, 폴리믹신 B를 생산하는 것으로 확인된 F4 균주의 PmxE의 모듈 3의 경우 Dab을 D 형으로 변환하는 광학이성질체화(epimerization, E) 도메인을 가지고 있었는데, 이는 기존 보고에 의해 폴리믹신 B의 구조중 3번째 사슬의 Dab이 L 형으로 알려져 있으나 F4 균주가 생산하는 폴리믹신 B의 3번째 Dab은 D 형일 가능성이 있음을 의미한다.
The polymyxin B and E biosynthetic gene cluster is Paenibacillus previously identified by the present inventors. Similar to the polymyxin biosynthesis gene derived from the polymyxa E681 strain, it was composed in the order of pmxA , pmxB , pmxC , pmxD and pmxE (see Fig. 2), and as a result of amino acid sequence analysis of the polypeptide encoded by the gene, predicted polymic It was found that the structures of the gods were polymyxins B and E (see FIG. 3). However, module 3 of PmxE of the F4 strain, which was confirmed to produce polymyxin B, had an optical isomerization (E) domain that converts Dab into D form, which is the structure of polymyxin B according to previous reports. Dab of the third chain is known as L-type, but the third Dab of polymyxin B produced by the F4 strain is likely to be D-type.

본 발명의 폴리믹신 생합성 효소 및 이를 암호화하는 폴리뉴클레오티드는 펩티드 항생물질인 폴리믹신 B 및 E를 모균 또는 이종 숙주균을 이용하여 효율적으로 생합성 하거나 유도체 등 새로운 항생물질을 개발하는데 사용할 수 있다는 유리한 효과를 가지고 있다.
The polymyxin biosynthetic enzyme of the present invention and the polynucleotide encoding the same have an advantageous effect of being able to efficiently biosynthesize polymyxins B and E, which are peptide antibiotics, using parental or heterologous host bacteria, or to develop new antibiotics such as derivatives. Have.

도 1은 상업적으로 이용가능한 폴리믹신 B 표준 시료(상부 판넬)와 F4 균주에서 생산된 폴리믹신(하부 판넬)에 대한 LC 및 MS 분석결과를 보여주는 일련의 그래프이다.
도 2는 ATCC21830 균주 및 F4 균주로부터 유래한 폴리믹신 합성효소를 암호화하는 유전자 클러스터의 구조 및 상기 폴리믹신 합성효소의 각 소단위체의 상세한 구조를 보여주는 개요도이다.
도 3은 ATCC21830 균주 및 F4 균주로부터 생산되는 폴리믹신 합성효소 각각의 도메인 구조로부터 예측된 폴리믹신의 구조를 보여주는 개요도이다.1 is a series of graphs showing LC and MS analysis results of commercially available polymyxin B standard samples (upper panel) and polymyxins (lower panel) produced in F4 strain.
2 is a schematic diagram showing the structure of a gene cluster encoding polymyxin synthase derived from ATCC21830 strain and F4 strain, and detailed structures of each subunit of the polymyxin synthase.
3 is a schematic diagram showing the structure of polymyxin predicted from the domain structure of each of the polymyxin synthase produced from the ATCC21830 strain and the F4 strain.

이하, 본 발명의 이해를 돕기 위하여 바람직한 실시예를 제시한다. 그러나, 하기의 실시예는 본 발명을 보다 쉽게 이해하기 위하여 제공되는 것일 뿐, 실시예에 의해 본 발명의 내용이 한정되는 것은 아니다.
Hereinafter, a preferred embodiment is presented to aid the understanding of the present invention. However, the following examples are provided for easier understanding of the present invention, and the contents of the present invention are not limited by the examples.

실시예Example 1: F4 1: F4 분리균Isolate 확보 및 동정 Secure and sympathize

<1-1> 균분리<1-1> bacterial separation

충남 공주지역에서 채집한 약 3g의 토양시료를 멸균수 20 ml에 현탁하여 30분간 200 rpm으로 진탕한 후 80℃에서 15분 열처리하였다. 10^-1~10^-3 희석액을 준비하여 Tryptic soy agar(TSA) 플레이트에 100 ul씩 도말하였다. 30℃에서 5일 배양 후 자란 콜로니 중 포자를 형성하며 대장균과 식물병원성 곰팡이인 Rhizoctonia solani 및 Fusarium oxysporom에 대해 우수한 길항력을 나타내는 균을 선발하여 동정하는 과정을 통해 F4 균주를 선발하였다.
About 3g of soil samples collected from Gongju, Chungcheongnam-do were suspended in 20 ml of sterile water, shaken at 200 rpm for 30 minutes, and heat treated at 80°C for 15 minutes. A 10 ^-1 to 10 ^-3 dilution was prepared, and 100 ul was plated on Tryptic soy agar (TSA) plates. It forms spores among colonies grown after 5 days incubation at 30℃, and Rhizoctonia solani and Fusarium, which are E. coli and phytopathogenic fungi. F4 strains were selected through the process of selecting and identifying bacteria showing excellent antagonism against oxysporom.

<1-2> 균동정<1-2> Identification of fungi

16S rDNA 염기서열 해독 결과 아래와 같은 염기서열(서열번호 17) 얻었으며 BLAST 분석을 통해 Paenibacillus polymyxa균으로 동정되었다. 근연균주와 비교한 결과 P. polymyxa E681균과는 99.6%(1360/1365) 상동성을 나타냈고 P. polymyxa ATCC21830 균과는 99.8%(1332/1335) 상동성을 나타냈다. 상기 균이 신규 균주로 판명됨에 따라, 본 발명자들은 상기 균주를 P. polymyxa F4로 명명하고, 상기 F4 균주를 2010년 3월 16일자로 한국생명공학연구원내 생물자원센터 유전자은행에 수탁번호 KCTC11667BP로 기탁하였다.
As a result of decoding 16S rDNA nucleotide sequence, the following nucleotide sequence (SEQ ID NO: 17) was obtained, and Paenibacillus was obtained through BLAST analysis. It was identified as polymyxa bacteria. As a result of comparison with the relative strain, P. polymyxa E681 showed 99.6% (1360/1365) homology and P. polymyxa ATCC21830 showed 99.8% (1332/1335) homology. As the bacterium was found to be a new strain, the present inventors named the strain P. polymyxa F4, and the F4 strain was designated as the accession number KCTC11667BP to the Gene Bank of the Biological Resource Center in the Korea Research Institute of Bioscience and Biotechnology on March 16, 2010. Deposited.

F4 균주 16S F4 strain 16S rDNArDNA 염기서열 (1365 Sequence (1365) bpbp ))

TAGCGGCGGACGGGTGAGTAACACGTAGGCAACCTGCCCACAAGACAGGGATAACTACCGGAAACGGTAGCTAATACCCGATACATCCTTTTCCTGCATGGGAGAAGGAGGAAAGACGGAGCAATCTGTCACTTGTGGATGGGCCTGCGGCGCATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGCCAGGGAAGAACGTCTTGTAGAGTAACTGCTACAAGAGTGACGGTACCTGAGAAGAAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGCTCTTTAAGTCTGGTGTTTAATCCCGAGGCTCAACTTCGGGTCGCACTGGAAACTGGGGAGCTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGGCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGCTAGGTGTTAGGGGTTTCGATACCCTTGGTGCCGAAGTTAACACATTAAGCATTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCTGACCGGTCTAGAGATAGACCTTTCCTTCGGGACAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATGCTTAGTTGCCAGCAGGTCAAGCTGGGCACTCTAAGCAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGGCCGGTACAACGGGAAGCGAAATCGCGAGGTGGAGCCAATCCTAGAAAAGCCGGTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGTCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCACGAGAGTTTACAACACCCGAAGTCGGTGGGGTAACCCGC
TAGCGGCGGACGGGTGAGTAACACGTAGGCAACCTGCCCACAAGACAGGGATAACTACCGGAAACGGTAGCTAATACCCGATACATCCTTTTCCTGCATGGGAGAAGGAGGAAAGACGGAGCAATCTGTCACTTGTGGATGGGCCTGCGGCGCATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGCCAGGGAAGAACGTCTTGTAGAGTAACTGCTACAAGAGTGACGGTACCTGAGAAGAAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGCTCTTTAAGTCTGGTGTTTAATCCCGAGGCTCAACTTCGGGTCGCACTGGAAACTGGGGAGCTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGGCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGCTAGGTGTTAGGGGTTTCGATACCCTTGGTGCCGAAGTTAACACATTAAGCATTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCTGACCGGTCTAGAGATAGACCTTTCCTTCGGGACAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATGCTTAGTTGCCAGCAGGTCAAGCTGGGCACTCTAAGCAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGGCCGGTACAACGGGAAGCGAAATCGCGAGGTGGAGCCAATCCTAGAAAAGCCGGTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGTCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCACGAGAGTTTACAACACCCGAAGTCGGTGGGGTAACCCGC

실시예Example 2: F4 균주로부터 2: from F4 strain 폴리믹신의Polymyxin 분리 및 동정 Separation and sympathy

Choi 등이 사용한 배지(J. Bacteriol. 191(10): 3350-3358, 2009)를 이용하여 F4 균주를 호기적 조건에서 30℃, 200 rpm으로 3일간 배양하여, 원심분리(7000 rpm, 10 min) 후 상등액을 얻었다.
Using the medium ( J. Bacteriol . 191(10): 3350-3358, 2009) used by Choi et al., the F4 strain was incubated for 3 days at 30° C. and 200 rpm under aerobic conditions, and centrifuged (7000 rpm, 10 min. ) To obtain the supernatant.

LC/MS 분석은 써모 일렉트론사(Thermo electron Co., USA)의 고압 액체 크로마토그래피 시스템과 이온 스펙트로미터(ion spectrometer) 장비를 이용하였으며 역상컬럼(YMC Hydrosphere C18 column)에서 0.1% 포름산(formic acid)이 첨가되어 있는 아세토니트릴(acetonitril)과 물을 용매로 하여 용출속도 0.2 ㎖/min의 조건으로 분석하였다. 분석 결과 F4 균주는 시그마사로부터 구입한 Polymyxin B(제품번호 P0972)의 경우와 동일한 (M+H)⁺이온분자량 1203.7, (M+2H)²⁺이온분자량 602.6 및 (M+3H)³⁺이온분자량 402.0의 값을 확인하였다(도1). 따라서 F4 균주는 폴리믹신 B를 생산함을 확인하였다. 농촌진흥청 농업유전자원센터에서 구매한 ATCC21830 균주는 이미 polymyxin E를 생산하는 균으로 이미 보고된 바 있어(Koyama, Y. et al., J. Antibiot ., 3: 457-458, 1950) 본 발명에서는 LC/MS 분석을 실시하지 않았다.
LC/MS analysis was performed using a high-pressure liquid chromatography system and ion spectrometer of Thermo electron Co., USA, and 0.1% formic acid in a YMC Hydrosphere C18 column. Using the added acetonitrile and water as solvents, the analysis was performed under conditions of an elution rate of 0.2 ml/min. As a result of analysis, the F4 strain was the same as that of Polymyxin B (product number P0972) purchased from Sigma (M+H) ⁺ ionic molecular weight 1203.7, (M+2H) ²⁺ ionic molecular weight 602.6 and (M+3H) ³⁺ ions The molecular weight of 402.0 was confirmed (Fig. 1). Therefore, it was confirmed that the F4 strain produced polymyxin B. The ATCC21830 strain purchased from the Agricultural Genetic Resource Center of the Rural Development Administration has already been reported as a fungus producing polymyxin E (Koyama, Y. et al ., J. Antibiot ., 3: 457-458, 1950) In the present invention, LC/MS analysis was not performed.

실시예Example 3: 3: 폴리믹신Polymyxin B와 B and 폴리믹신Polymyxin E 생합성 유전자의 염기서열 결정 E biosynthetic gene base sequence determination

<3-1> 라이브러리 제작<3-1> Library production

ATCC21830 균주 및 F4 균주를 tryptic soy broth(TSB) 배지에서 16시간 배양하여 Genome Analysis, A laboratory manual Vol. III Cloning systems(CSHL Press, Cold Spring Harbor, NY, USA)에 기술된 방법에 따라 염색체 DNA를 분리한 다음 삽입 DNA 절편의 크기가 약 40 kb인 포스미드 라이브러리를 키트(CopyControl^TM fosmid library production kit, Epicentre Biotechnologies, Madison, WI, USA)를 사용하여 제작하였다. 두 균주로부터 제작된 두 세트의 포스미드 라이브러리에서 임의로 선택된 각각 1500개씩의 클론으로부터 폴리믹신 생합성 유전자군을 함유하는 클론들을 콜로니 교잡(colony hybridization) 방법으로 선별하였다. 이때 프로브로는 pmxC의 3' 말단부위 약 300 bp와 pmxD의 5' 말단부위 약 400 bp를 포함하는 약 700 bp 크기의 PCR 산물을 pmxCC primer(서열번호: 18, 5'-gagcttttctgaaggatg-3'), pmxDN primer(서열번호: 19, 5'-ggtcgttacgtcgttgttc-3')와 Dig-labelling kit(Roche Diagnostics Corporation)를 이용하여 제작하여 사용하였다. 각 라이브러리로부터 1차로 양성반응을 보이는 32개씩의 클론을 선별하였고 이들로부터 포스미드 DNA를 추출한 후 앞에서 사용한 probe를 이용하여 DNA Dot blotting을 실시하였다. 양성반응을 보이는 클론을 13개씩 선정하여 양말단의 염기서열 결정하였고 이를 근거로 삽입된 DNA 단편이 폴리믹신 생합성 유전자군의 어느 부위를 포함하는지 확인하였다.
ATCC21830 strain and F4 strain were cultured in tryptic soy broth (TSB) medium for 16 hours to obtain Genome Analysis, A laboratory manual Vol. Isolation of chromosomal DNA according to the method described in III Cloning systems (CSHL Press, Cold Spring Harbor, NY, USA), and then a ^{fosmid library with a size of about 40 kb of the inserted DNA fragment was prepared as a kit (CopyControl TM} fosmid library production kit, Epicentre Biotechnologies, Madison, WI, USA). Clones containing the polymyxin biosynthetic gene group were selected from each of 1,500 clones randomly selected from two sets of fosmid libraries produced from two strains by a colony hybridization method. The probe is a PCR product of about 700 bp size pmxCC primer comprising a 3 'terminal region of about 300 bp and 5 pmxD' terminal region of about 400 bp of pmxC (SEQ ID NO: 18, 5'-gagcttttctgaaggatg-3 ') , pmxDN primer (SEQ ID NO: 19, 5'-ggtcgttacgtcgttgttc-3') and a Dig-labelling kit (Roche Diagnostics Corporation) were used to prepare and use. From each library, 32 clones showing a positive reaction in the first place were selected, fosmid DNA was extracted from them, and DNA dot blotting was performed using the probe previously used. Thirteen positive clones were selected and sequenced at both ends, and based on this, it was confirmed which part of the polymyxin biosynthetic gene group the inserted DNA fragment contains.

<3-2> 염기서열 분석<3-2> nucleotide sequence analysis

양 말단 염기서열 정보로부터 ATCC21830 균주와 F4 균주의 전체 폴리믹신 생합성 유전자군을 포함하는 클론을 각각 2개씩 선정하여 유전자군 전체 염기서열을 분석,확보하였다. 염기서열 해독은 산탄식 시퀀싱 방법(shotgun sequencing strategy)을 이용하여 수행하였다. 염색체 DNA 절편 준비는 VCX-500 초음파 처리기(Sonics, Newtown, CT, USA)로 19% 강도, 펄스(pulse) on/off 시간은 0.3/3초 조건으로 6회 반복하여 실시하였다. 라이브러리에 삽입될 DNA 절편은 1.5-2 kb 크기를 회수하여 평활말단화 작업을 거쳐 사용하였으며, pUC19 벡터의 Sma I 제한효소 위치에 삽입하였다. 플라스미드 라이브러리를 위한 반응물을 대장균 DH5α에 열충격 방법으로 도입한 다음 X-gal/IPTG/Amp(Ampicillin)이 함유된 LB 한천 배지에 도말하였다. 백색 재조합 콜로니를 LB(Amp) 액체 배지가 든 96 딥-웰 플레이트(deep-well plate)에 접종 후 37℃의 항온 배양기에서 250 rpm으로 16 시간 동안 진탕 배양하여 세포를 회수한 뒤 표준 방법으로 플라스미드 DNA를 분리 정제하였다. From the nucleotide sequence information at both ends, two clones each containing the entire polymyxin biosynthetic gene group of the ATCC21830 strain and the F4 strain were selected, and the entire nucleotide sequence of the gene group was analyzed and secured. The nucleotide sequence decoding was performed using a shotgun sequencing strategy. Preparation of chromosomal DNA fragments was performed 6 times with a VCX-500 sonicator (Sonics, Newtown, CT, USA) with a 19% intensity and a pulse on/off time of 0.3/3 seconds. The DNA fragment to be inserted into the library was recovered and used after smoothing the 1.5-2 kb size. Sma of the pUC19 vector Inserted at the I restriction enzyme site. The reactants for the plasmid library were introduced into E. coli DH5α by a heat shock method and then plated on LB agar medium containing X-gal/IPTG/Amp (Ampicillin). White recombinant colonies were inoculated into a 96 deep-well plate containing LB (Amp) liquid medium, and then incubated with shaking at 250 rpm for 16 hours in an incubator at 37°C to recover the cells and then plasmid using the standard method. DNA was separated and purified.

DNA 염기서열 결정 반응은 BigDye^TM terminator cycle sequencing kit(Applied Biosystems, CA, USA)를 이용하여 수행하였으며, 반응물은 ABI 3700 및 3730 DNA analyzer(Applied Biosystems, Foster City, CA, USA)에서 분석하였다. 서열분석 결과 파일 처리는 Phred/Phrap/Consed 프로그램을 이용하였다 (http://www.phrap.org). 모든 결과 파일은 Phred로 처리하여 염기서열을 할당하였으며, 양질의 결과만을 취한 뒤 벡터 서열을 차폐하였다. 서열 합체 작업에는 Phrap을 사용하였으며 콘티그 확인과 편집 및 프라이머 설계 작업에는 Consed를 사용하였다. 플라스미드 클론 약 200개의 염기서열을 양방향으로 분석하여 유전자군 전체 길이의 약 7 배수에 해당하는 염기서열을 확보하였고 이를 합체하여 약 41 kb 길이의 서열을 완성하였다(서열번호 20). 전체 염기서열에 대한 ORF 분석 결과 F4 균주의 경우 폴리믹신 B 생합성효소를 암호화하는 유전자는 서열번호 7의 핵산서열을 갖는 pmxA, 서열번호 8의 핵산서열을 갖는 pmxB, 서열번호 10의 핵산서열을 갖는 pmxC, 서열번호 11의 핵산서열을 갖는 pmxD 및 서열번호 9의 핵산서열을 갖는 pmxE로 구성된 유전자클러스터인 것으로 밝혀졌다. 또한, ATCC21830 균주의 경우, 폴리믹신 E 생합성효소를 암호화하는 유전자는 서열번호 12의 핵산서열을 갖는 pmxA, 서열번호 13의 핵산서열을 갖는 pmxB, 서열번호 15의 핵산서열을 갖는 pmxC, 서열번호 16의 핵산서열을 갖는 pmxD 및 서열번호 14의 핵산서열을 갖는 pmxE로 구성된 유전자클러스터인 것으로 밝혀졌다. 상기 구조는 기존에 규명된 P. polymyxa E681 균주 유래의 폴리믹신 생합성 유전자의 구조와 동일한 것이다.The DNA sequencing reaction was ^{performed using the BigDye TM} terminator cycle sequencing kit (Applied Biosystems, CA, USA), and the reactions were analyzed by ABI 3700 and 3730 DNA analyzers (Applied Biosystems, Foster City, CA, USA). The sequence analysis result file was processed using the Phred/Phrap/Consed program (http://www.phrap.org). All result files were treated with Phred to assign nucleotide sequences, and only good results were taken, and vector sequences were masked. Phrap was used for sequence consolidation, and Consed was used for contig identification and editing and primer design. About 200 nucleotide sequences of about 200 plasmid clones were analyzed in both directions to obtain a nucleotide sequence corresponding to about 7 times the total length of the gene group, and merged to complete a sequence of about 41 kb in length (SEQ ID NO: 20). In the case of ORF analysis F4 strain of the entire base sequence polymyxin B gene coding for biosynthetic enzyme SEQ ID NO: having pmxB, the nucleic acid sequence of SEQ ID NO: 10 having a pmxA, the nucleic acid sequence of SEQ ID NO: 8 with a nucleic acid sequence of the seven It was found to be a gene cluster consisting of pmxC , pmxD having the nucleic acid sequence of SEQ ID NO: 11 and pmxE having the nucleic acid sequence of SEQ ID NO: 9. In the case of the ATCC21830 strain, polymyxin E gene coding for biosynthetic enzyme pmxA having a nucleic acid sequence of SEQ ID NO: 12, SEQ ID NO: 13 pmxC, SEQ ID NO: having pmxB, SEQ ID NO: 15 nucleic acid sequence having a nucleic acid sequence of 16 It was found to be a gene cluster consisting of pmxD having the nucleic acid sequence of and pmxE having the nucleic acid sequence of SEQ ID NO: 14. The structure is the same as the structure of the polymyxin biosynthesis gene derived from the previously identified P. polymyxa E681 strain.

이렇게 완성한 F4 균주와 ATCC21830 균주의 폴리믹신 생합성 유전자의 염기서열을 변환하여 PmxA, PmxB, PmxE의 아미노산 서열을 확보한 후 이를 분석한 결과 두 균의 PmxA는 각각 4959, 4966개(각각 서열번호 1 및 4), PmxB는 두 균 모두 1102개(각각 서열번호 2 및 5), PmxE는 각각 6282, 5789개(각각 서열번호 3 및 6)의 아미노산 서열로 이루어진 것으로 분석되었다.
As a result of analyzing the amino acid sequences of PmxA, PmxB, and PmxE by converting the base sequence of the polymyxin biosynthesis gene of the F4 strain and ATCC21830 strain thus completed, PmxA of the two strains was 4959 and 4966, respectively (SEQ ID NO: 1 and 4), PmxB was analyzed to consist of 1102 amino acid sequences in both bacteria (SEQ ID NOs: 2 and 5, respectively), and PmxE consists of 6282 and 5789 amino acid sequences (SEQ ID NOs: 3 and 6, respectively).

실시예Example 4: 4: 폴리믹신Polymyxin 생합성 유전자의 염기서열로부터 From the nucleotide sequence of a biosynthetic gene 폴리믹신Polymyxin 구조 예측 Structure prediction

Ansari 등의 방법(Nucleic acids Res. 32: W405-W413, 2004)으로 패니바실러스 ATCC21830 균주와 F4 균주의 폴리믹신 생합성 유전자군을 분석한 결과 모두 도 2과 같이 pmxA , pmxB , pmxC , pmxD 및 pmxE 유전자 구조로 구성되어 있음을 알 수 있었고, PmxA, PmxB 및 PmxE의 각 모듈의 A 도메인은 도 2에 기술된 바와 같이 2,4-diaminobutyric acid(Dab), 류신(Leu), 트레오닌(Thr) 및 페닐알라닌(Phe)의 아미노산을 인식하고 있음을 알 수 있었다. 이러한 분석을 통해 예측된 폴리믹신의 구조는 도 3에 나타난 것과 같이 폴리믹신 B와 E임을 알 수 있었고, 이는 실시예 2에서 보여준 결과와 일치하였다. 그런데 폴리믹신 B를 생산하는 것으로 확인된 F4균의 PmxE의 모듈 3의 경우 DAB을 D 형으로 변환하는 광학이성질체화(epimerization, E) 도메인을 가지고 있다. 이는 기존 보고에 의해 폴리믹신 B의 구조중 3번째 사슬의 Dab이 L 형으로 알려져 있으나 F4 균주가 생산하는 폴리믹신 B의 3번째 Dab은 D 형일 가능성이 있음을 의미한다. The method of Ansari et al. ( Nucleic acids Res . 32: W405-W413, 2004) as a result of analyzing the polymyxin biosynthetic gene group of Panibacillus ATCC21830 strain and F4 strain, as shown in FIG. 2, all of them pmxA , pmxB , pmxC , pmxD and pmxE It was found that it was composed of a gene structure, and the A domain of each module of PmxA, PmxB and PmxE was 2,4-diaminobutyric acid (Dab), leucine (Leu), threonine (Thr), and It was found that it recognizes the amino acid of phenylalanine (Phe). The structure of the predicted polymyxin through this analysis was found to be polymyxin B and E as shown in FIG. 3, which is consistent with the results shown in Example 2. However, module 3 of PmxE of F4 bacteria, which has been confirmed to produce polymyxin B, has an optical isomerization (E) domain that converts DAB into D form. This means that although the Dab of the 3rd chain of the structure of polymyxin B is known as L-type by the previous report, the 3rd Dab of polymyxin B produced by the F4 strain is likely to be D-type.

이상의 결과에서 폴리믹신 B와 E를 생산하는 생합성 효소 유전자군은 폴리믹신 A 생합성 효소 유전자군(Choi et al ., J. Bacteriol. 191(10): 3350-3358, 2009)의 경우와 마찬가지로 pmxA , pmxB , pmxC , pmxD 및 pmxE의 5개의 유전자로 이루어져 있고 이중 생합성 효소를 코딩하는 유전자는 pmxA, pmxB 및 pmxE임을 알 수 있었다. 또한 폴리믹신 생합성 과정에 참여하는 모듈의 순서도 PmxE, PmxA 및 PmxB 순서로서 서로 동일하다. 따라서 이상에서 밝혀진 3종 폴리믹신 생합성 효소의 경우로 미루어 볼 때 다양한 종류의 폴리믹신을 생산하는 생합성 효소는 대부분 이와 같은 구조를 가지고 있을 것으로 예상된다.
From the above results, the biosynthetic enzyme gene group that produces polymyxin B and E is the polymyxin A biosynthetic enzyme gene group (Choi et al ., J. Bacteriol . As in the case of 191(10): 3350-3358, 2009), it was found that the genes were composed of five genes: pmxA , pmxB , pmxC , pmxD and pmxE , and the genes encoding double biosynthetic enzymes were pmxA , pmxB and pmxE . In addition, the order of modules participating in the polymyxin biosynthesis process is the same as PmxE, PmxA, and PmxB. Therefore, considering the three polymyxin biosynthetic enzymes identified above, most biosynthetic enzymes that produce various types of polymyxin are expected to have such a structure.

본 발명의 폴리믹신 생합성 효소 및 이를 암호화하는 폴리뉴클레오티드는 종래에 이를 생산하는 균주 자체로부터 분리하거나 화학합성에만 의존하던 폴리믹신 B 및 E의 생산을 유전공학 기술을 활용하여 외래 숙주세포로부터 더욱 경제적으로 수행할 수 있게 함으로써, 다중 항생제 내성균에 의한 감염증 치료 분야에 매우 효율적으로 사용될 수 있으며, 독성이 더 적은 폴리믹신 유도체의 제조에 활용될 수 있다.
The polymyxin biosynthetic enzyme of the present invention and the polynucleotide encoding the same are more economical from foreign host cells by utilizing genetic engineering technology to produce polymyxins B and E, which were conventionally isolated from the strain itself producing it or depend only on chemical synthesis. By allowing it to be performed, it can be very efficiently used in the field of treatment of infectious diseases caused by multiple antibiotic-resistant bacteria, and can be utilized in the production of polymyxin derivatives with less toxicity.

한국생명공학연구원Korea Research Institute of Bioscience and Biotechnology KCTC11667KCTC11667 2010031620100316

<110> Korea Research Institute of Bioscience and Biotechnolgy <120> Polymyxin B or E synthetase and gene cluster thereof <130> 11p-12-54 <160> 20 <170> PatentIn version 3.5 <210> 1 <211> 4959 <212> PRT <213> Paenibacillus polymyxa F4 <400> 1 Met Ala Phe Glu Lys Glu Thr Leu Phe Trp Asn Glu Lys Phe Gly Ser 1 5 10 15 Asp Asp Tyr Thr Leu Thr Arg Leu Pro Tyr Ser Lys Ala Pro Ser Ser 20 25 30 Leu Ala Pro Ile Met Thr Thr Val Gly Gly Thr Leu Ser Glu Glu Val 35 40 45 Ala Gln Arg Val Leu Gln Met Ser Lys Gly Ala Pro Leu Ala Thr Phe 50 55 60 Met Ile Leu Leu Ala Gly Val Gln Ser Leu Leu His Lys Tyr Thr Gly 65 70 75 80 Ala Ser Asp Ile Leu Val Gly Met Pro Val Val Arg Lys Pro Thr Glu 85 90 95 Thr Arg Arg Ser Val Asn His Thr Ile Ile Leu Lys Asn Ser Leu Ser 100 105 110 Ala Gly Ala Thr Phe Lys Thr Leu Leu Ser Glu Leu Arg Thr Ser Leu 115 120 125 Pro Glu Ala Ile Gln His Gln His Ile Pro Phe Leu Lys Met Thr Glu 130 135 140 Lys Leu Asp Leu Gln Tyr Ala Gly Gly Ile Pro Val Val His Thr Leu 145 150 155 160 Val Ser Leu Lys Glu Leu His Leu Asp Glu Ile Gly Gln Asn Val Val 165 170 175 Thr Asp Cys Ser Phe Glu Phe Ser Leu Thr Gly Gly Thr Ile Gln Leu 180 185 190 Ala Leu Ser Tyr Asn Glu His Leu Tyr Asp Ser Glu Phe Met Thr Arg 195 200 205 Val Val Gly His Leu Asn Arg Leu Leu Ala Val Gly Leu His Glu Leu 210 215 220 Glu Leu Asp Ile Val Arg Ala Asp Met Leu Ser Glu Asp Glu Lys Phe 225 230 235 240 Gln Leu Leu Gln Ser Phe Asn Asp Thr Glu Lys Asp Tyr Pro Arg Asp 245 250 255 Arg Thr Ile His Gln Leu Val Glu Glu Gln Ala Lys Arg Val Pro Glu 260 265 270 Ala Thr Ala Val Val Phe Glu Gly Arg Arg Leu Ser Tyr Ala Glu Leu 275 280 285 Asn Glu Arg Ala Asn Arg Leu Ala Arg Thr Leu Arg Ser Val Gly Val 290 295 300 Leu Pro Asn Gln Leu Val Gly Met Met Ala Arg Arg Ser Leu Glu Thr 305 310 315 320 Val Val Gly Ile Leu Ala Val Leu Lys Ala Gly Gly Ala Tyr Val Pro 325 330 335 Ile Asp Pro Glu Tyr Pro Glu Glu Arg Ile Arg Tyr Ile Leu Glu Asn 340 345 350 Ser Asn Ala Gln Leu Leu Leu Thr Gln Arg Glu Leu Leu Gln Gln Val 355 360 365 Pro Phe Glu Gly Thr Val Val Ala Leu Asp Asp Glu Gln Ala Tyr Ser 370 375 380 Asp Asp Gly Ser Asn Leu Glu Pro Ala Ser Gly Pro Asn Asp Leu Ala 385 390 395 400 Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly Lys Pro Lys Gly Val Met 405 410 415 Leu Glu His Arg Gly Leu Val Ser Leu Lys Leu Thr Phe Ala His Thr 420 425 430 Leu His Thr Thr Glu Gln Asp Arg Val Leu Gln Phe Ala Ser Leu Ser 435 440 445 Phe Asp Ala Ser Cys Trp Glu Met Phe Asn Ala Leu Tyr Phe Gly Ala 450 455 460 Thr Leu Tyr Ile Pro Ser Thr Glu Thr Ile Leu Asp Asp Gln Leu Phe 465 470 475 480 Glu Arg Phe Met Asn Glu His Ala Ile Thr Ile Ala Thr Leu Pro Pro 485 490 495 Thr Tyr Ala Ala Tyr Leu Asn Ser Asp Arg Leu Pro Ser Leu Ser Arg 500 505 510 Leu Ile Thr Ala Gly Ser Ala Val Ser Ala Glu Phe Val Gln Gln Trp 515 520 525 Lys Asp Lys Val Gln Tyr Tyr Asn Ala Tyr Gly Pro Thr Glu Ala Ser 530 535 540 Ile Ala Thr Ser Val Trp Ala Ala Ser Thr His Asp Thr Glu Arg Arg 545 550 555 560 Ala Ile Pro Ile Gly Arg Pro Ile Trp Asn His Arg Leu Tyr Ile Leu 565 570 575 Gly Ala Gln Asn Gln Leu Ala Pro Ile Gly Val Glu Gly Glu Leu Cys 580 585 590 Ile Ala Gly Val Gly Leu Ala Arg Gly Tyr Trp Asn Arg Pro Asp Leu 595 600 605 Thr Ala Glu Lys Phe Ile Glu His Pro Phe Glu Pro Gly Glu Arg Leu 610 615 620 Tyr Arg Thr Gly Asp Leu Ala Arg Trp Leu Pro Asp Gly Asn Ile Glu 625 630 635 640 Tyr Leu Gly Arg Ile Asp His Gln Val Lys Ile Arg Gly Phe Arg Ile 645 650 655 Glu Ile Gly Glu Ile Glu Glu Gln Leu Leu Lys Ile Asp Ser Val Gln 660 665 670 Glu Thr Ile Val Ile Ala Arg Glu Gly Lys Ser Gly Gln Glu Leu Cys 675 680 685 Ala Tyr Leu Val Ala Gly Arg Pro Leu Thr Leu Gly Glu Leu Arg Ser 690 695 700 Ala Leu Ala Gln Lys Leu Pro Asn Tyr Met Ile Pro Ala His Phe Val 705 710 715 720 Gln Leu Pro Gln Met Pro Leu Thr Pro Asn Asp Lys Ile Asp Arg Lys 725 730 735 Ala Leu Pro Ala Pro Glu Gly Asn Ala Leu Thr Gly Gly Ala Tyr Val 740 745 750 Ala Pro Arg Asn Glu Ala Glu Arg Thr Leu Ala Asp Val Trp Gln Ala 755 760 765 Val Leu Asn Ala Asp Arg Val Gly Val Thr Asp His Phe Phe Glu Leu 770 775 780 Gly Gly Asp Ser Ile Lys Ser Ile Gln Val Ser Ser Arg Leu His Gln 785 790 795 800 Ala Gly Tyr Lys Leu Glu Ile Arg Asp Leu Phe Lys Tyr Pro Thr Ile 805 810 815 Ser Gln Leu Ser Leu His Val Lys Pro Ile Gly Arg Thr Ile Asp Gln 820 825 830 Gly Glu Ile Thr Gly Glu Thr Ala Leu Met Pro Ile Gln His Trp Phe 835 840 845 Phe Glu Ser Ser Phe Ala Asp Pro His His Phe Asn Gln Ser Val Met 850 855 860 Leu Tyr Arg Lys Glu Arg Phe Asp Glu Glu Thr Val Arg Gln Val Leu 865 870 875 880 Gln Lys Leu Ala Glu His His Asp Ala Leu Arg Met Val Phe Arg Lys 885 890 895 Thr Glu Gln Gly Phe Ser Ala Arg Asn Arg Ala Ile Gln Glu Gly Gly 900 905 910 Leu Phe Thr Leu Asp Val Phe Asp Phe Lys Asp Ala Glu Asn Thr Ala 915 920 925 Gln Ala Val Glu Ala Lys Ala Thr Asp Ile Gln Ala Gly Ile Asp Leu 930 935 940 Glu Asn Gly Pro Leu Ala Lys Ala Gly Leu Phe Arg Cys Ala Asp Gly 945 950 955 960 Asp His Leu Leu Leu Ala Val His His Ala Val Val Asp Gly Val Ser 965 970 975 Trp Arg Ile Leu Met Glu Asp Phe Ala Leu Gly Tyr Glu Gln Ala Gly 980 985 990 Lys Ser Glu Glu Ile Arg Phe Pro Ala Lys Thr Asp Ala Tyr Arg Thr 995 1000 1005 Trp Ser Glu Gln Leu Ala Ala Tyr Ala Gln Ser Pro Glu Met Ala Lys 1010 1015 1020 Glu Arg Ala Tyr Trp Gln Ala Val Glu Gln Ile Ala Val Pro Ala Val 1025 1030 1035 1040 Pro Lys Asp Leu Glu Ala Asp Val Thr Thr Gln Gln Asp Ser Glu Ser 1045 1050 1055 Leu Phe Val Arg Leu Thr Pro Glu Glu Thr Glu Leu Leu Leu Lys Arg 1060 1065 1070 Val His Arg Ala Tyr Asn Thr Glu Met Asn Asp Ile Leu Val Thr Ala 1075 1080 1085 Leu Gly Ile Ala Val Arg Lys Trp Thr Gly His Glu Arg Val Arg Ile 1090 1095 1100 Asn Leu Glu Gly His Gly Arg Glu Ser Ile Gly Thr Asp Ile Asp Ile 1105 1110 1115 1120 Thr Arg Thr Val Gly Trp Phe Thr Thr Lys Phe Pro Val Val Leu Glu 1125 1130 1135 Pro Glu Thr Asp Arg Asp Leu Ala Tyr Gln Ile Lys Gln Val Lys Glu 1140 1145 1150 Ser Leu Arg Arg Ile Pro Asn Lys Gly Leu Gly Tyr Gly Val Cys Arg 1155 1160 1165 Tyr Leu Ser Lys Ser Glu Asp Gly Phe Val Trp Gly Ala Glu Pro Glu 1170 1175 1180 Ile Asn Phe Asn Tyr Leu Gly Gln Phe Gly Asp Asp Val Asn Gln Asp 1185 1190 1195 1200 Glu Ile Gly Ile Ser Ser Tyr Ser Ser Gly Ser Pro Ala Ser Asp Arg 1205 1210 1215 Gln Ala Arg Ser Phe Val Leu Asp Ile Asn Gly Met Val Leu Asp Gly 1220 1225 1230 Ala Leu Ser Leu Asp Leu Ser Tyr Ser Arg Lys Gln Tyr Arg Lys Glu 1235 1240 1245 Thr Met Glu Ala Phe Ala Gln Arg Leu Glu Gln Ser Leu Arg Glu Leu 1250 1255 1260 Ile Thr His Cys Ala Gly Lys Glu Asn Thr Glu Leu Thr Pro Ser Asp 1265 1270 1275 1280 Val Gln Phe Lys Gly Leu Thr Ile Ala Glu Leu Glu Gln Ile Ala Gln 1285 1290 1295 Arg Ser Gly His Leu Gly Glu Ile Glu Asn Ile Tyr Ser Leu Thr Pro 1300 1305 1310 Met Gln Lys Gly Met Trp Phe His Ser Ala Leu Asp Arg Gln Thr Ala 1315 1320 1325 Ala Tyr Phe Glu Gln Thr Arg Phe Thr Met Arg Gly Ala Leu Asp Val 1330 1335 1340 Gln Leu Phe Glu Arg Ser Trp Met Glu Leu Ala Lys Arg His Leu Val 1345 1350 1355 1360 Leu Arg Ala Asn Phe Val Lys Gly Pro Ala Gly Glu Pro Leu Gln Ile 1365 1370 1375 Ile Tyr Arg Asp Lys Pro Val Gly Phe Glu Tyr Glu Glu Leu Leu His 1380 1385 1390 Leu Gln Ala Asp Glu Lys Gln Ala Tyr Leu Asp Lys Lys Ala Glu Asp 1395 1400 1405 Asp Lys Leu Arg Gly Phe Asp Met Glu His Asp Ala Leu Val Arg Val 1410 1415 1420 Thr Ile Leu Arg Thr Glu Glu Gln Ser Tyr His Val Leu Trp Ser Phe 1425 1430 1435 1440 Gln His Ile Leu Met Asp Gly Trp Cys Leu Pro Gln Leu Thr Gln Glu 1445 1450 1455 Leu Phe Glu Thr Tyr Ser Ala Leu Ala Ser Gly Lys Gln Pro Ala Gly 1460 1465 1470 Asp Lys Gly Ser Asp Tyr Gly Ala Tyr Ile Glu Trp Leu Glu Lys Gln 1475 1480 1485 Asp Asp Gln Ala Ala Ser Gly Tyr Trp Thr Ala Phe Leu Ala Gly Tyr 1490 1495 1500 Glu Gly Gln Thr Val Leu Pro Gly Gln Lys Glu Pro Ala Pro Asn Gly 1505 1510 1515 1520 Arg Phe Thr Ala Asp His Val Thr Ala Glu Leu Gly Lys Asp Leu Ser 1525 1530 1535 Glu Arg Met Asp Arg Val Ala Lys Gln Arg Leu Val Thr Val Asn Thr 1540 1545 1550 Leu Leu Gln Ala Ala Trp Gly Val Met Leu Gln Lys Tyr Asn Gly Thr 1555 1560 1565 Asn Asp Ala Val Phe Gly Ser Val Val Ala Gly Arg Pro Ala Glu Ile 1570 1575 1580 Pro Gly Ile Glu Ser Met Ile Gly Leu Phe Ile Asn Thr Val Pro Val 1585 1590 1595 1600 Arg Val Thr Ser Glu Ala Asp Thr Val Phe Ala Asp Leu Met Ala Lys 1605 1610 1615 Leu Gln Glu Arg Ala Leu Glu Ser Gly Arg Tyr Asp Tyr Tyr Pro Leu 1620 1625 1630 Tyr Glu Ile Gln Ala Arg Ser Val Gln Lys Gln Asn Leu Ile Asn His 1635 1640 1645 Ile Ile Ala Phe Glu Asn Tyr Pro Val Asp Glu Gln Met Glu Gln Ala 1650 1655 1660 Gly Asp Gln Gln His Gly Asp Leu Thr Ile Ala Asp Val Gln Met Glu 1665 1670 1675 1680 Glu Gln Thr Asn Tyr Asn Phe Asn Val Thr Val Val Pro Gly Val Glu 1685 1690 1695 Ile Glu Ile Arg Phe Asp Phe Asn Ala Glu Val Phe Asp Lys Asp Ser 1700 1705 1710 Ile Glu Arg Leu Lys Gly His Leu Val His Leu Leu Glu Gln Val Thr 1715 1720 1725 Asp Asn Pro Glu Ile Thr Val Gly Glu Leu Glu Leu Val Thr Glu Ala 1730 1735 1740 Glu Lys Ala Asp Leu Leu Gly Arg Phe Asn Asp Thr Thr Thr Glu Phe 1745 1750 1755 1760 Pro Arg Gly Lys Thr Leu Ile Gln Leu Phe Glu Glu Gln Ala Glu Arg 1765 1770 1775 Ile Pro Asp Ala Ala Ala Ile Ser Leu Asn Glu Gln Glu Leu Thr Tyr 1780 1785 1790 Arg Glu Leu Asn Glu Arg Val Asn Arg Leu Ala Arg Thr Leu Arg Ser 1795 1800 1805 His Gly Ile Ser Lys Gly Arg Leu Val Ala Ile Met Ala Glu Arg Ser 1810 1815 1820 Ile Glu Met Val Val Gly Met Leu Ala Ala His Lys Ala Gly Ala Ala 1825 1830 1835 1840 Tyr Val Pro Ile Asp Pro Glu Tyr Pro Glu Glu Arg Ile Arg Phe Leu 1845 1850 1855 Ile Glu Asp Ser Gly Ala Gln Val Met Leu Thr Gln Ser Arg Leu Arg 1860 1865 1870 Glu Arg Leu Ala Gly Ser Asp Ser Val Ile Leu Leu Asp Asp Glu Ser 1875 1880 1885 Phe Tyr His Glu Asp Gly Thr Asn Leu Asn Pro Gly Thr Glu Ala Thr 1890 1895 1900 Asp Leu Ala Cys Val Ile Tyr Thr Ser Gly Thr Thr Gly Lys Pro Lys 1905 1910 1915 1920 Gly Asn Leu Val Ser His Arg Asn Ile Val Arg Ile Val Arg Asn Thr 1925 1930 1935 Asn Tyr Ile Asp Ile Thr Glu Arg Asp His Val Leu Gln Leu Ser Ser 1940 1945 1950 Tyr Ser Phe Asp Gly Ala Ile Phe Asp Ile Phe Gly Ala Leu Thr Asn 1955 1960 1965 Gly Ala Arg Leu Val Leu Val Pro Arg Glu Thr Leu Leu Glu Ile Val 1970 1975 1980 Arg Leu Ala Asp Leu Ile Gln Arg Glu Arg Ile Ser Val Met Leu Ile 1985 1990 1995 2000 Thr Thr Ala Phe Phe Asn Val Leu Val Asp Val Asn Val Asp Cys Leu 2005 2010 2015 Arg Asp Val Arg Ala Ile Leu Phe Gly Gly Glu Arg Val Ser Val Gly 2020 2025 2030 His Val Arg Lys Ala Leu Ala His Ile Gly Pro Gly Arg Leu Asn His 2035 2040 2045 Leu Tyr Gly Pro Ser Glu Ser Thr Val Tyr Thr Thr Tyr Leu Pro Val 2050 2055 2060 Asp Phe Val Asp Glu Ser Ala Val Thr Val Pro Ile Gly Arg Pro Ile 2065 2070 2075 2080 Ser Asn Thr Lys Val Tyr Ile Val Asp Ser Arg Asn Lys Leu Leu Pro 2085 2090 2095 Ile Gly Val Ala Gly Glu Leu Cys Val Gly Gly Glu Gly Leu Val Arg 2100 2105 2110 Gly Tyr Asn Asn Arg Pro Glu Leu Thr Ala Glu Lys Phe Val Asp Asn 2115 2120 2125 Pro Phe Val Leu Gly Glu Arg Met Tyr Arg Thr Gly Asp Leu Ala Lys 2130 2135 2140 Trp Leu Pro Asp Gly Thr Ile Glu Tyr Val Gly Arg Thr Asp Asp Gln 2145 2150 2155 2160 Val Lys Ile Arg Gly Phe Arg Ile Glu Leu Gly Glu Ile Glu Ala Gln 2165 2170 2175 Leu Gln Lys Val Glu Gly Ile Arg Lys Ala Thr Val Phe Ala Arg Glu 2180 2185 2190 Asp Ala Ser Gly Glu Lys Gln Leu Cys Ala Tyr Tyr Glu Ala Asp Arg 2195 2200 2205 Glu Leu Pro Ala Ala Glu Leu Lys Ser Val Leu Ser Gln Glu Leu Pro 2210 2215 2220 Ala Tyr Met Ile Pro Ala Tyr Leu Ile Gln Leu Glu Gln Leu Pro Leu 2225 2230 2235 2240 Thr Thr Asn Gly Lys Val Asp Arg Arg Ser Leu Pro Ala Pro Glu Glu 2245 2250 2255 Ser Leu Gln Pro Gly Glu Glu Arg Thr Pro Pro Arg Thr Pro Leu Glu 2260 2265 2270 Ala Ser Leu Ala Gly Ile Trp Lys Ser Val Leu Gly Leu Glu His Ile 2275 2280 2285 Gly Val His Asp Asn Phe Phe Asp Leu Gly Gly His Ser Leu Arg Ala 2290 2295 2300 Thr Thr Leu Val Ser Lys Val His Gln Glu Leu Asn Val Glu Leu Pro 2305 2310 2315 2320 Leu Arg Asp Val Phe Arg Tyr Ser Thr Ile Glu Glu Met Ala Leu Ala 2325 2330 2335 Ile Ser Arg Ile Gly Glu Gln Ser Phe Ser Ser Ile Pro Leu Ala Gly 2340 2345 2350 Ala Arg Ala Tyr Tyr Pro Leu Ser Ser Ala Gln Lys Arg Leu Phe Ile 2355 2360 2365 Leu Asn Gln Leu Glu Gly Ala Asp Gln Ser Tyr Asn Met Pro Gly Val 2370 2375 2380 Leu Leu Leu Glu Gly Ser Ile Asp Arg Ser Leu Leu Glu Lys Ala Phe 2385 2390 2395 2400 Arg Gly Leu Ile Ala Arg His Glu Thr Leu Arg Thr Gly Phe Glu Ile 2405 2410 2415 Val Gln Gly Glu Ala Val Gln Arg Ile Tyr Glu Ser Val Asp Phe Ala 2420 2425 2430 Val Gly Tyr Arg His Val Ser Glu Glu Glu Ala Pro Glu Val Val Gln 2435 2440 2445 Ala Phe Ile Arg Pro Phe Asp Leu Ala Lys Pro Pro Leu Leu Arg Ala 2450 2455 2460 Glu Leu Val Glu Leu Ala Ala Glu Arg Tyr Leu Leu Met Phe Asp Met 2465 2470 2475 2480 His His Ile Val Ser Asp Gly Val Ser Met Asp Val Leu Val Glu Glu 2485 2490 2495 Leu Val Arg Leu Tyr Gly Gly Glu Ser Leu Glu Pro Leu Arg Ile Gln 2500 2505 2510 Tyr Lys Asp Tyr Ala Val Trp Gln Gln Ser Asp Glu Gln Lys Val Gln 2515 2520 2525 Leu Lys Arg Glu Glu Ala Tyr Trp Leu Asp Arg Tyr Arg Gly Glu Leu 2530 2535 2540 Pro Val Leu Glu Met Pro Thr Asp Tyr Pro Arg Pro Ala Val Gln Ser 2545 2550 2555 2560 Phe Glu Gly Gln Thr Leu Thr Ser Phe Val Asp Glu Ala Thr Asn Glu 2565 2570 2575 Gly Leu Lys Gln Leu Ala Ala Gln Arg Gly Thr Thr Leu Tyr Met Val 2580 2585 2590 Leu Leu Ala Ala Tyr Thr Val Leu Leu His Lys Tyr Thr Gly Gln Asp 2595 2600 2605 Asp Leu Ile Val Gly Thr Ser Ile Ala Gly Arg Thr His Gly Glu Thr 2610 2615 2620 Gln Pro Leu Ile Gly Met Phe Val Asn Thr Leu Ala Leu Arg Asn Tyr 2625 2630 2635 2640 Pro Ala Ser Glu Lys Thr Phe Leu Ser Tyr Leu Glu Glu Val Lys Glu 2645 2650 2655 Thr Thr Leu Gly Ala Tyr Glu His Gln Asn Tyr Pro Phe Glu Glu Leu 2660 2665 2670 Val Asp Lys Val Gln Val Ser Arg Asp Leu Ser Arg Asn Pro Leu Phe 2675 2680 2685 Asp Thr Met Phe Ser Leu Gln Asn Leu Glu Asp Lys Glu Phe Glu Leu 2690 2695 2700 Glu Gly Leu Lys Leu Ser Pro Tyr Pro Ser Glu Tyr Gly Thr Ala Lys 2705 2710 2715 2720 Phe Asp Leu Ser Val Asp Val Thr Glu Glu Asn Gly Gly Leu Glu Cys 2725 2730 2735 Ser Phe Glu Phe Ala Thr Ala Leu Tyr Lys Glu Ser Thr Ile Arg Arg 2740 2745 2750 Leu Ser Thr His Phe Gly His Leu Leu Val Thr Ile Val Ser Arg Pro 2755 2760 2765 Asp Ala Lys Ile Ala Glu Leu Asn Leu Leu Thr Ala Glu Glu Lys Glu 2770 2775 2780 Gln Ile Leu Gly Ala Phe Asn Pro Ala Gln Pro Glu Ala Ala Pro Ala 2785 2790 2795 2800 Ser Ala Phe His Arg Leu Phe Glu Glu Gln Ala Glu Arg Thr Pro Glu 2805 2810 2815 Ala Glu Ala Val Val Tyr Glu Asn Asp Arg Leu Thr Tyr Ala Glu Leu 2820 2825 2830 Asn Glu Arg Ala Asn Arg Leu Ala Ala Thr Leu Arg Ala Ser Gly Ile 2835 2840 2845 Gly Arg Glu Ala Ile Val Gly Ile Leu Ala Glu Arg Ser Val Asp Leu 2850 2855 2860 Leu Val Ala Val Leu Ala Val Trp Lys Val Gly Gly Ala Tyr Val Pro 2865 2870 2875 2880 Leu Asp Pro Asp Tyr Pro Ala Glu Arg Val Arg Phe Met Leu Glu Asp 2885 2890 2895 Ser Gly Ala Lys Val Leu Leu Thr Gln Thr Pro Leu Arg Glu Arg Ala 2900 2905 2910 Glu Ala Trp Leu Gly Glu Glu Glu Leu Ala Leu Ala Ala Val Leu Tyr 2915 2920 2925 Leu Asp Asp Glu Ala Ser Tyr Ser Asp Glu Arg Ala Asn Ala Ser Val 2930 2935 2940 Val Ser Gly Lys Leu Thr Asp Ala Val Asp Asp Gly Asp Glu Ser His 2945 2950 2955 2960 Gln Asn Val Gly Thr Asp Ser Phe His Glu Ala Arg Pro Glu Asp Leu 2965 2970 2975 Ala Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly Lys Pro Lys Gly Val 2980 2985 2990 Met Ile Glu His Arg Ser Leu Val Asn Thr Ala Ala Gly Tyr Arg Arg 2995 3000 3005 Glu Tyr Arg Leu Asp Gln Phe Ala Val Arg Leu Leu Gln Leu Ala Ser 3010 3015 3020 Phe Ser Phe Asp Val Phe Val Gly Asp Ile Ala Arg Thr Leu Tyr Asn 3025 3030 3035 3040 Gly Gly Thr Met Val Ile Val Pro Lys Asp Asp Arg Ile Asp Pro Ser 3045 3050 3055 Arg Leu His His Trp Ile Glu Arg Glu Arg Val Thr Ile Phe Glu Ser 3060 3065 3070 Thr Pro Ala Leu Ile Val Pro Phe Leu Glu Tyr Val Tyr Glu Gln Gln 3075 3080 3085 Leu Asp Met Ser Arg Leu Glu Leu Leu Ile Thr Ser Ser Asp Ser Cys 3090 3095 3100 Ser Val Ala Asp Tyr Arg Thr Leu Gln Glu Arg Phe Gly Ser Leu Phe 3105 3110 3115 3120 Arg Ile Ile Asn Ala Tyr Gly Val Thr Glu Ala Ala Ile Asp Ser Ser 3125 3130 3135 Phe Tyr Asp Glu Glu Leu Ala Lys Leu Pro Gln Thr Gly Asn Val Pro 3140 3145 3150 Ile Gly Lys Ala Trp Leu Asn Ala Lys Phe Tyr Ile Val Asp Ala His 3155 3160 3165 Leu Asn Pro Val Pro Val Gly Val Leu Gly Glu Leu Val Ile Gly Gly 3170 3175 3180 Val Gly Val Ala Arg Gly Tyr Leu Asn Arg Pro Glu Leu Thr Glu Glu 3185 3190 3195 3200 Lys Phe Val Asp Ser Pro Phe Ala Glu Gly Glu Arg Leu Tyr Arg Thr 3205 3210 3215 Gly Asp Leu Ala Arg Trp Met Glu Asp Gly Asn Val Asp Phe Ile Gly 3220 3225 3230 Arg Ile Asp Asn Gln Ala Lys Ile Arg Gly Tyr Arg Ile Glu Thr Gly 3235 3240 3245 Glu Ile Glu Ser Gln Leu Leu Arg Val Glu Gly Val Arg Glu Ala Val 3250 3255 3260 Val Leu Val Arg Ser Asp Ala Asn Gly Gln Lys Ala Leu Cys Ala Tyr 3265 3270 3275 3280 Tyr Thr Pro Asp Thr Gly Ala Glu Leu Ala Val Asn Asp Leu Arg Gly 3285 3290 3295 Ala Leu Ala Gln Glu Leu Pro Gly Tyr Met Ile Pro Ser Tyr Phe Val 3300 3305 3310 Glu Leu Glu Arg Leu Pro Leu Thr Pro Asn Gly Lys Ile Asp Arg Lys 3315 3320 3325 Ala Leu Pro Ala Pro Glu Gly Glu Ala Gly Ser Gly Thr Glu Tyr Val 3330 3335 3340 Ala Pro Arg Asn Glu Leu Glu Thr Lys Leu Ala Ala Ile Trp Gln Glu 3345 3350 3355 3360 Val Leu Gly Leu Ala Lys Glu Ile Gly Val His Asp Asn Phe Phe Asp 3365 3370 3375 Ile Gly Gly His Ser Leu Arg Ala Thr Thr Leu Val Ser Lys Ile His 3380 3385 3390 Lys Glu Leu Asn Val Asp Leu Pro Leu Arg Asp Val Phe Arg His Ser 3395 3400 3405 Thr Ile Glu Ser Met Ala Ala Ala Ile Ser Arg Leu Asp Glu Gln Thr 3410 3415 3420 Phe Val Ala Ile Pro Val Ala Asp Asp Arg Glu Val Tyr Pro Gln Ser 3425 3430 3435 3440 Phe Ala Gln Lys Arg Leu Phe Ile Leu Asn Gln Leu Glu Gly Ala Glu 3445 3450 3455 Leu Ser Tyr Asn Met Pro Glu Ala Met Leu Leu Glu Gly Ala Leu Asp 3460 3465 3470 Arg Ala Arg Phe Glu Glu Ala Phe Arg Lys Leu Val Ala Arg His Glu 3475 3480 3485 Thr Leu Arg Thr Gly Phe Glu Met Val Asp Gly Glu Ala Ser Gln Arg 3490 3495 3500 Ile Tyr Gln Asp Val Asn Phe Ala Val Glu Phe Tyr Arg Ala Asp Glu 3505 3510 3515 3520 Gln Glu Ala Glu Glu Thr Val Arg Arg Phe Val Arg Pro Phe Asp Leu 3525 3530 3535 Ala Lys Pro Pro Leu Leu Arg Val Gly Leu Val Glu Leu Ala Pro Glu 3540 3545 3550 Arg His Ile Leu Met Tyr Asp Met His His Ile Ile Ser Asp Gly Val 3555 3560 3565 Ser Met Glu Ile Phe Val Glu Glu Phe Val Arg Leu Tyr Gly Gly Glu 3570 3575 3580 Gln Leu Glu Pro Leu Arg Ile Gln Tyr Lys Asp Tyr Thr Val Trp Gln 3585 3590 3595 3600 His Ser Gln Glu Gln Lys Glu Arg Leu Gln Arg Gln Glu Ala Tyr Trp 3605 3610 3615 Leu Asp Met Phe Gln Gly Glu Leu Pro Val Leu Glu Met Pro Thr Asp 3620 3625 3630 Tyr Pro Arg Pro Ala Val Gln Ser Tyr Glu Gly Gln Thr Leu Glu Phe 3635 3640 3645 Phe Phe Asp Ala Ser Lys Thr Asp Gly Leu Lys Gln Leu Ala Ser Glu 3650 3655 3660 Thr Gly Thr Thr Leu Phe Met Val Leu Leu Ala Ala Tyr Asn Val Leu 3665 3670 3675 3680 Leu His Lys Tyr Ser Gly Gln Glu Asp Val Ile Val Gly Thr Pro Ile 3685 3690 3695 Ala Gly Arg Asn His Gly Asp Val Gln Pro Leu Ile Gly Ile Phe Leu 3700 3705 3710 Asn Thr Leu Ala Ile Arg Ser Tyr Pro Ala Ser Glu Lys Thr Phe Leu 3715 3720 3725 Ser Tyr Leu Asn Glu Val Lys Glu Thr Thr Leu His Ala Phe Glu His 3730 3735 3740 Gln Asn Tyr Pro Phe Glu Glu Leu Val Asp Lys Val Gln Val Thr Arg 3745 3750 3755 3760 Asp Leu Ser Arg Asn Pro Leu Phe Asp Thr Leu Phe Thr Met Gln Asn 3765 3770 3775 Thr Glu Asn Glu Glu Phe Glu Leu Glu Gly Leu Arg Leu Ile Pro Tyr 3780 3785 3790 Pro Ser Ala Leu Asp Thr Ala Lys Phe Asp Ile Ser Leu Asp Val Gly 3795 3800 3805 Glu Glu Asn Gly Gly Leu Asp Tyr Ser Phe Glu Tyr Ala Thr Ala Leu 3810 3815 3820 Tyr Lys Arg Glu Thr Ile Glu Arg Leu Ala Lys His Tyr Glu Gln Leu 3825 3830 3835 3840 Leu Val Thr Ile Val Ser Arg Pro Asp Ala Lys Ile Ala Glu Leu Asn 3845 3850 3855 Leu Leu Thr Ala Glu Glu Lys Glu Gln Ile Leu Gly Ala Phe Asn Pro 3860 3865 3870 Ala Gln Pro Glu Ala Ala Pro Ala Ala Ala Phe His Arg Leu Phe Glu 3875 3880 3885 Glu Gln Ala Glu Arg Thr Pro Glu Ala Glu Ala Val Val Tyr Glu Asn 3890 3895 3900 Asp Arg Leu Thr Tyr Ala Glu Leu Asn Glu Arg Ala Asn Arg Leu Ala 3905 3910 3915 3920 Ala Thr Leu Arg Ala Ser Gly Ile Gly Arg Glu Thr Ile Val Gly Ile 3925 3930 3935 Leu Ala Glu Arg Ser Val Asp Leu Leu Val Ala Val Leu Ala Val Trp 3940 3945 3950 Lys Ala Gly Gly Ala Tyr Val Pro Leu Asp Pro Asp Tyr Pro Ala Glu 3955 3960 3965 Arg Val Arg Phe Met Leu Glu Asp Ser Gly Ala Lys Val Leu Leu Thr 3970 3975 3980 Gln Thr Pro Leu Arg Glu Arg Ala Glu Ala Trp Leu Gly Glu Glu Glu 3985 3990 3995 4000 Leu Ala Leu Ala Ala Val Leu Tyr Leu Asp Asp Glu Ala Ser Tyr Ser 4005 4010 4015 Glu Glu Arg Ala Asn Ala Ser Val Val Ser Gly Lys Leu Thr Asp Ala 4020 4025 4030 Val Asp Asp Gly Asp Glu Ser His Gln Asn Val Gly Thr Asp Ser Phe 4035 4040 4045 His Glu Ala Arg Pro Glu Asp Leu Ala Tyr Val Ile Tyr Thr Ser Gly 4050 4055 4060 Thr Thr Gly Lys Pro Lys Gly Val Met Ile Glu His Arg Ser Leu Val 4065 4070 4075 4080 Asn Thr Ala Ala Gly Tyr Arg Arg Glu Tyr Arg Leu Asp Gln Phe Pro 4085 4090 4095 Val Arg Leu Leu Gln Leu Ala Ser Phe Ser Phe Asp Val Phe Val Gly 4100 4105 4110 Asp Ile Ala Arg Thr Leu Tyr Asn Gly Gly Thr Met Val Ile Val Pro 4115 4120 4125 Lys Asp Asp Arg Ile Asp Pro Ser Arg Leu His His Trp Ile Glu Arg 4130 4135 4140 Glu Arg Val Thr Ile Phe Glu Ser Thr Pro Ala Leu Ile Val Pro Phe 4145 4150 4155 4160 Leu Asp Tyr Val His Glu Gln Gln Leu Asp Met Ser Trp Met Glu Leu 4165 4170 4175 Leu Ile Thr Ser Ser Asp Ser Cys Ser Val Ala Asp Tyr Arg Thr Leu 4180 4185 4190 Gln Glu Arg Phe Gly Ser Leu Phe Arg Ile Ile Asn Ala Tyr Gly Val 4195 4200 4205 Thr Glu Ala Ala Ile Asp Ser Ser Phe Tyr Asp Glu Pro Leu Thr Lys 4210 4215 4220 Leu Pro Gln Thr Gly Asn Val Pro Ile Gly Lys Ala Trp Leu Asn Ala 4225 4230 4235 4240 Lys Phe Tyr Ile Val Asp Ala His Leu Asn Pro Val Pro Val Gly Val 4245 4250 4255 Leu Gly Glu Leu Val Ile Gly Gly Val Gly Val Ala Arg Gly Tyr Leu 4260 4265 4270 Asn Arg Pro Glu Leu Thr Glu Glu Lys Phe Val Asp Ser Pro Phe Ala 4275 4280 4285 Ala Gly Glu Arg Leu Tyr Arg Thr Gly Asp Leu Ala Arg Trp Met Glu 4290 4295 4300 Asp Gly Asn Val Asp Phe Ile Gly Arg Ile Asp Asn Gln Thr Lys Ile 4305 4310 4315 4320 Arg Gly Tyr Arg Ile Glu Thr Gly Glu Ile Glu Ser Gln Leu Leu Arg 4325 4330 4335 Val Glu Gly Val Arg Glu Ala Val Val Leu Val Arg Ser Asp Ala Asn 4340 4345 4350 Gly Gln Lys Ala Leu Cys Ala Tyr Tyr Thr Pro Asp Thr Gly Ala Glu 4355 4360 4365 Leu Ala Val Asn Asp Leu Arg Ser Ala Leu Ala Gln Glu Leu Pro Gly 4370 4375 4380 Tyr Met Ile Pro Ser His Phe Val Glu Thr Glu Arg Leu Pro Leu Thr 4385 4390 4395 4400 Pro Asn Gly Lys Ile Asp Arg Lys Ala Leu Pro Ala Pro Glu Gly Glu 4405 4410 4415 Ala Gly Ser Gly Thr Glu Tyr Val Ala Pro Arg Asn Glu Leu Glu Thr 4420 4425 4430 Lys Leu Ala Ala Ile Trp Gln Glu Val Leu Gly Leu Ala Lys Glu Ile 4435 4440 4445 Gly Val His Asp Asn Phe Phe Asp Ile Gly Gly His Ser Leu Arg Ala 4450 4455 4460 Thr Thr Leu Ala Gly Lys Val Phe Lys Glu Leu Asn Val Asn Leu Pro 4465 4470 4475 4480 Leu Arg Asp Val Phe Arg His Ser Thr Ile Ala Ala Met Ala Glu Ala 4485 4490 4495 Ile Ala Arg Met Glu Arg Arg Glu His Glu Ala Ile Pro Gln Ala Glu 4500 4505 4510 Glu Arg Glu Tyr Tyr Pro Leu Ser Ser Ala Gln Lys Arg Leu Phe Ile 4515 4520 4525 Gln His Thr Leu Asp Gly Ala Asp Gln Leu Tyr Asn Met Pro Glu Leu 4530 4535 4540 Val Gln Val Glu Gly Glu Phe Asp Leu Asp Arg Leu Glu Ala Ala Leu 4545 4550 4555 4560 Arg Lys Leu Ile Thr Arg His Glu Ser Leu Arg Thr Gly Phe Glu Ile 4565 4570 4575 Val Lys Ala Glu Ala Val Gln Arg Ile Tyr Pro Gln Val Asp Phe Ala 4580 4585 4590 Ile Glu His His Gln Ala Asp Lys Glu Asp Ala Ala Gln Ile Glu Gln 4595 4600 4605 Ile Val Arg Ser Phe Val Arg Pro Phe Asp Leu Gly Lys Pro Pro Leu 4610 4615 4620 Leu Arg Ala Gly Val Ile Glu Leu Glu Pro Asn Leu His Ile Leu Leu 4625 4630 4635 4640 Phe Asp Met His His Met Val Ser Asp Gly Val Ser Met Ala Ile Val 4645 4650 4655 Ile Asp Glu Phe Ser Ser Phe Tyr Ala Gly Glu Glu Leu Pro Pro Leu 4660 4665 4670 Arg Ile Gln Tyr Lys Asp Tyr Ala Val Trp Gln Gln Ser Lys Ala His 4675 4680 4685 Arg Glu Arg Ile Gly Arg Gln Glu Ala Tyr Trp Leu Gln Thr Phe Glu 4690 4695 4700 Gly Glu Leu Pro Thr Ala Asp Leu Pro Met Asp Tyr Glu Arg Ser Ala 4705 4710 4715 4720 Ala Arg Ser Tyr Glu Gly Ala His Leu Glu Phe Asp Val Glu Ala Ser 4725 4730 4735 Leu Ser Ala Arg Leu Arg Glu Leu Ala Ala Glu Arg Glu Ser Thr Leu 4740 4745 4750 Phe Met Val Leu Leu Ala Ala Tyr Thr Val Leu Leu Ser Lys Tyr Ser 4755 4760 4765 Gly Gln Glu Asp Leu Val Val Gly Thr Pro Val Ala Gly Arg Thr Asn 4770 4775 4780 Ala Asp Leu Glu Pro Val Ile Gly Met Phe Val Asn Thr Leu Ala Ile 4785 4790 4795 4800 Arg Asn Arg Pro Ser Gly Asp Lys Thr Phe Leu Ser Tyr Leu Glu Glu 4805 4810 4815 Val Lys Glu Thr Ala Leu Gly Ala Phe Glu Asn Gln Asp Tyr Pro Phe 4820 4825 4830 Glu Glu Leu Val Glu Arg Leu Asn Val Lys Arg Glu Pro Gly Arg Phe 4835 4840 4845 Pro Leu Phe Asp Ala Val Phe Asp Leu Gln Asn Ile Glu Glu Arg Asp 4850 4855 4860 Ala Glu Leu Glu Gly Val Ser Leu Lys Thr Tyr Glu Leu Asp His Leu 4865 4870 4875 4880 Glu Glu Ala Lys Phe Asp Leu Thr Leu Phe Met Tyr Glu Asn Asn Gly 4885 4890 4895 Ala Leu Ser Gly Gly Phe Phe Tyr Ala Thr Lys Leu Phe Lys Glu Ala 4900 4905 4910 Met Ile Arg Thr Leu Thr Glu Asp Tyr Leu Arg Val Leu Ser Gln Ile 4915 4920 4925 Ala Glu Asn Pro Gln Leu Glu Leu Ser Arg Ile Glu Cys His Lys Pro 4930 4935 4940 Ala Ala Gly Ala Lys Ser Ala Val Asp Thr Ile Glu Phe Ala Phe 4945 4950 4955 <210> 2 <211> 1102 <212> PRT <213> Paenibacillus polymyxa F4 <400> 2 Met Lys Ser Leu Phe Glu Lys Glu Glu Arg Tyr Trp Ser Gly Lys Phe 1 5 10 15 Asp Ala Asp Asp Ser Leu Ser Phe Leu Pro Tyr Ser Gln Ser Ser Lys 20 25 30 Leu Ser Ala Asp Gly Glu Ala Ala Ala Glu Pro Gly Leu Leu His Arg 35 40 45 Thr Leu Pro Ser Glu Leu Ser Glu Arg Ile Ile Cys Leu Ala Asn Gly 50 55 60 Ser Asp Leu Ala Leu Tyr Met Ile Val Leu Ala Gly Val Lys Ser Leu 65 70 75 80 Leu Phe Lys Tyr Thr Gly Arg Asp Gln Val Leu Val Gly Met Pro Ser 85 90 95 Tyr Ser Ala Asp Pro Asp Gly Thr Pro Pro Pro His Asp Ile Leu Val 100 105 110 Ile Lys Thr Ser Val Ser Arg Gln Thr Thr Leu Lys Thr Leu Leu Gly 115 120 125 Gly Ile Lys Ala Ser Ile Gly Glu Ala Leu Glu His Gln His Leu Pro 130 135 140 Phe Arg Lys Met Val Glu Pro Leu His Leu Asp Tyr Thr Gly Asp Gly 145 150 155 160 Leu Pro Val Val Asn Thr Val Val Ser Phe Ala Pro Ile His Pro Glu 165 170 175 Pro Leu Gly Asn Arg Val Ala Ala Asp Thr Val Phe Arg Phe Asp Arg 180 185 190 Gln Asn His Ser Ile Glu Leu Glu Ile Ser Phe Asp Gly Gln Arg Tyr 195 200 205 Glu Arg Ala Phe Val Glu Gln Ala Ala Asp His Leu Val Arg Leu Leu 210 215 220 Ser Val Leu Leu Phe Gln Pro Asp Leu Glu Leu Gly Gln Ala Asp Val 225 230 235 240 Leu Ser Pro Asp Glu Arg Glu Thr Leu Leu Lys Arg Phe Asn Asp Thr 245 250 255 Glu Thr Glu Phe Glu Arg Gly Lys Thr Ile Tyr Gly Leu Phe Glu Glu 260 265 270 Gln Ala Glu Leu Tyr Pro Asp Asn Val Ala Ala Val Met Asn Glu Arg 275 280 285 Gln Leu Thr Tyr Arg Glu Leu Asn Glu Arg Ser Asn Arg Leu Ala Arg 290 295 300 Lys Leu Arg Glu Thr Gly Val Glu Ala Asp Gln Leu Val Ala Ile Leu 305 310 315 320 Ala Glu Arg Ser Leu Asp Met Val Val Gly Ile Leu Ala Ile Leu Lys 325 330 335 Ala Gly Gly Ala Tyr Val Pro Val Asp Pro Asp Tyr Pro Glu Glu Arg 340 345 350 Ile Arg Phe Met Ile Glu Asp Ser Gly Ala Pro Leu Leu Leu Ile Gln 355 360 365 Lys His Leu His Glu Lys Thr Asp Phe Ala Gly Thr Arg Leu Glu Leu 370 375 380 Asp Asp Phe Val Trp Gly Asp Arg Gly Ala Asp Ser Ala Asp Val Arg 385 390 395 400 Asp Ala Ser Asn Leu Glu Pro Ile Ser Gly Pro Gly Asn Leu Ala Tyr 405 410 415 Val Ile Tyr Thr Ser Gly Thr Thr Gly Arg Pro Lys Gly Thr Leu Ile 420 425 430 Glu His Lys Asn Val Val Arg Leu Leu Phe Asn Asp Lys Asn Leu Phe 435 440 445 Asp Phe Gly Pro Ser Asp Thr Trp Thr Leu Phe His Ser Phe Cys Phe 450 455 460 Asp Phe Ser Val Trp Glu Met Tyr Gly Ala Leu Leu Tyr Gly Gly Lys 465 470 475 480 Leu Val Ile Val Pro Pro Leu Thr Ala Lys Asn Pro Ala Asp Phe Leu 485 490 495 Ala Leu Leu Gly Arg Glu Gln Val Thr Ile Leu Asn Gln Thr Pro Thr 500 505 510 Tyr Phe Tyr Gln Leu Leu Arg Lys Val Leu Ala Asp His Pro Tyr Asp 515 520 525 Leu Arg Ile Arg Asn Val Ile Phe Gly Gly Glu Ala Leu Ser Pro Leu 530 535 540 Leu Leu Lys Gly Phe Lys Thr Lys Tyr Pro Glu Thr Lys Leu Ile Asn 545 550 555 560 Met Tyr Gly Ile Thr Glu Thr Thr Val His Val Thr Tyr Lys Glu Ile 565 570 575 Thr Trp Val Glu Met Glu Ala Ala Lys Ser Asn Ile Gly Lys Pro Ile 580 585 590 Pro Thr Leu Ser Val Tyr Val Leu Asp Glu Asn Arg Ser Pro Val Pro 595 600 605 Ile Gly Val Ala Gly Glu Met Tyr Val Ala Gly Glu Gly Leu Ala Arg 610 615 620 Gly Tyr Leu Asn Arg Pro Asp Leu Thr Ala Glu Lys Phe Val Asp Ser 625 630 635 640 Pro Phe Ala Glu Gly Glu Lys Leu Tyr Arg Ser Gly Asp Leu Ala Ala 645 650 655 Trp Leu Pro Asp Gly Asn Ile Glu Tyr Leu Gly Arg Ile Asp His Gln 660 665 670 Val Lys Ile Arg Gly Tyr Arg Ile Glu Leu Asp Glu Ile Glu Thr Gln 675 680 685 Leu Leu Lys Ile Ala Ala Val Gln Glu Ala Lys Val Leu Asp Arg Asp 690 695 700 Asp Ala Asn Gly His Lys Gln Leu Val Ala Tyr Tyr Val Ala Glu Thr 705 710 715 720 Arg Leu Ala Ala His Glu Leu Lys Glu Glu Leu Ala Lys Gln Leu Pro 725 730 735 Gly Tyr Met Ile Pro Ser His Leu Val Gln Leu Ser Arg Met Pro Leu 740 745 750 Thr Pro Asn Gly Lys Ile Asp Arg Lys Ala Leu Pro Ala Pro Glu Glu 755 760 765 Ala Ala Ala Gly Gly Ala Glu Tyr Val Ala Pro Arg Thr Leu Leu Glu 770 775 780 Met Lys Ile Ala Arg Val Trp Gln Asp Thr Leu Gly Val Pro Gln Val 785 790 795 800 Gly Val Lys Asp Asn Phe Phe Glu Leu Gly Gly Asn Ser Leu Ser Leu 805 810 815 Met Arg Leu Val Gln Ala Val Tyr Asp Glu Thr Asp Ile Glu Ile Pro 820 825 830 Leu Asn Arg Gln Phe His Asn Leu Thr Val Glu Ala Met Ala Phe Gly 835 840 845 Glu Gly Asp Leu Gly Leu Asp Lys Gly Gly Asp Ser Phe Ile Lys Leu 850 855 860 Asn Lys Ala Gly Asp Leu Asn Val Phe Cys Phe Pro Pro Gly Ser Gly 865 870 875 880 Phe Gly Ile Gly Tyr Arg Glu Leu Ala Ser Arg Leu Asp Gly Arg Phe 885 890 895 Val Leu Tyr Gly Ile Asp Phe Ile Asp Asp Ala Val Asp Tyr Glu Ala 900 905 910 Met Leu Asn Arg Tyr Val Asp Glu Ile Val Arg Ile Gln Pro Glu Gly 915 920 925 Pro Tyr Val Leu Leu Gly Tyr Cys Phe Gly Gly Asn Leu Thr Phe Glu 930 935 940 Val Ala Lys Thr Met Glu Lys Arg Gly Tyr Ser Val Thr Asp Val Leu 945 950 955 960 Met Leu Asp Ser Trp Ile Lys Asp Thr Leu Thr Pro Ser Glu Thr Ser 965 970 975 Glu Lys Glu Leu Glu Glu Thr Leu Ala Asp Phe Asp Glu Glu Glu Lys 980 985 990 Glu Leu Met Ser Asn Pro Leu Val Arg Glu Arg Val His Arg Lys Val 995 1000 1005 Lys Ala Thr Leu Ala Tyr Glu Ala Gln Leu Ile Asn Ser Gly Thr Ile 1010 1015 1020 Pro Ala Arg Ile Tyr Glu Leu Ile Ala Lys Asp Ser Glu Ala Phe Arg 1025 1030 1035 1040 Leu Glu His Gln Leu Pro Ser Trp Arg Gly Ala Thr Thr Gln Ala Tyr 1045 1050 1055 Ala Asp Tyr Arg Leu Glu Gly Ala His Glu Glu Leu Leu Glu Leu Ala 1060 1065 1070 Arg Val Glu Glu Thr Ala Val Val Ile Arg Asp Ile Leu Glu Gln Val 1075 1080 1085 Lys Arg Arg Ile Glu Ala Glu Ala Gly Val Leu His Gly Ser 1090 1095 1100 <210> 3 <211> 6282 <212> PRT <213> Paenibacillus polymyxa F4 <400> 3 Met Arg Glu Asn Thr Asn Glu Gln Tyr Gly Leu Thr Gln Ala Gln Arg 1 5 10 15 Arg Ile Trp Phe Met Glu Ile Met Asn Pro Gly Thr Ser Ile Thr Met 20 25 30 Leu Ser Ala Thr Tyr Gln Ile Thr Gly Glu Ile Asn Thr Gln Leu Leu 35 40 45 Glu Gln Ala Ala Ala Glu Ile Val Lys Thr Tyr Asp Val Phe Arg Ile 50 55 60 Arg Ile Ser Gly Asp Leu Gln Asn Pro Thr Gln Trp Phe Glu Glu Pro 65 70 75 80 Glu Asn Val Gln Ala Arg Ile Ser Arg Leu Glu Ile Gly Thr Thr Glu 85 90 95 Gln Phe Tyr Ala Trp Val Lys Glu Val Ser Glu Lys Pro Ala Ser Val 100 105 110 Phe Asp Glu His Leu Tyr Gln Phe Thr Ile Ile His Phe Ala Asn Gly 115 120 125 Gln Val Trp Leu Asn Leu Thr Val Asn His Ile Ile Ala Asp Gly Leu 130 135 140 Ser Val Asn Ala Leu Leu His Ala Val Met Glu Lys Tyr Leu Glu Leu 145 150 155 160 Arg Lys Gly Ile Ser Ser Ser Tyr Gln Ala Pro Ser Tyr Leu Asp Tyr 165 170 175 Ile Ser Ala Glu Arg Glu Tyr Glu Gln Ser Gln Arg Tyr Gln Lys Gly 180 185 190 Lys Glu Tyr Trp Leu Thr Lys Tyr Asn Thr Leu Pro Glu Thr Thr Gly 195 200 205 Ile Lys Ser Tyr Pro Pro Phe Ser Ile Gly Ser Glu Ser Asn Lys Leu 210 215 220 Ala Ile Thr Leu Asp Gly Ser Arg Tyr Glu Arg Ile Leu Ala Phe Ser 225 230 235 240 Glu Gln Tyr Gln Val Ser Leu Tyr Thr Leu Phe Leu Ser Ala Met Tyr 245 250 255 Ala Leu Leu Tyr Lys Leu Thr Asp Ser Thr Asp Val Pro Val Gly Thr 260 265 270 Val Phe Ala Asn Arg Thr Ser Lys Lys Glu Lys Glu Thr Ile Gly Met 275 280 285 Phe Val Ser Thr Val Ala Thr Arg Ile Arg Leu Asn Pro Asp Arg His 290 295 300 Val Leu Ser Leu Ile Gln Thr Val Ser Lys Glu Asn Thr Ala Asp Leu 305 310 315 320 Arg Tyr Gln Lys Tyr Pro Tyr Asn Gln Leu Ile Gln Asp Leu Arg Glu 325 330 335 Gln His Gly Arg Asn Asp Leu Ser Gly Leu Phe Arg Thr Ser Leu Glu 340 345 350 Tyr Leu Pro Leu Lys Ile Val Glu Tyr Glu Glu Ile Lys Val Arg Leu 355 360 365 Glu Ala His Phe Ala Arg His Glu Met Asp Asp Leu Leu Leu Arg Phe 370 375 380 Asp His Met Leu Asn Glu Gly His Val Ile Leu His Ala Ser Tyr Arg 385 390 395 400 Thr Gly Leu Phe Glu Thr Ala Glu Ile Asp Arg Ile Met Glu Gln Tyr 405 410 415 Val Thr Val Leu Asp Gln Phe Leu Gln Thr Pro Glu Leu Pro Val Arg 420 425 430 Glu Ile Ser Leu Leu Ser Asp Glu Glu Arg His Arg Ile Leu Asn Val 435 440 445 Phe Asn Pro Pro Val Ala Gly Leu Ser Glu Gly Glu Ala Phe His Arg 450 455 460 Tyr Val Glu Lys Phe Ala Leu Glu Ile Pro Asp His Pro Ala Val Val 465 470 475 480 Tyr Met Asp Lys Gln Leu Thr Tyr Gly Glu Leu Asn Glu Arg Ala Glu 485 490 495 Arg Leu Ala Ser Leu Leu Arg Glu Gln Gly Val Gly Arg Glu Thr Ile 500 505 510 Thr Gly Ile Trp Ala Glu Arg Ser Val Glu Leu Leu Val Gly Val Leu 515 520 525 Ala Val Trp Lys Ala Gly Gly Ala Tyr Val Pro Leu Asp Pro Asp Tyr 530 535 540 Pro Ala Glu Arg Ile Glu Tyr Met Leu Ser Asp Ser Glu Ala Thr Val 545 550 555 560 Leu Leu Thr Gln Arg His Leu Leu Glu Arg Ala Gly Val Trp Leu Ala 565 570 575 Asp Asp Arg Leu Lys Leu Gln Ala Val Tyr Ala Met Asp Asp Glu Gln 580 585 590 Ile Tyr Asn Gly Asp Ala Leu Ala Val Glu Phe Glu Ser Ala Gly Ser 595 600 605 Ala Pro Gln Asp Leu Ala Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly 610 615 620 Arg Pro Lys Gly Val Met Ile Glu His Gly Ser Leu Val Asn Thr Ala 625 630 635 640 Asp Ala Tyr Arg Arg Glu Tyr Arg Leu Asp Gln Phe Pro Val Arg Leu 645 650 655 Leu Gln Leu Ala Ser Phe Ser Phe Asp Val Phe Val Gly Asp Ile Ala 660 665 670 Arg Thr Leu Tyr Asn Gly Gly Thr Met Val Ile Val Pro Lys Asp Asp 675 680 685 Arg Ile Asp Pro Asn Leu Leu Tyr Gly Trp Ile Arg Asp Gln Asn Ile 690 695 700 Thr Val Phe Glu Ser Thr Pro Ala Leu Ile Leu Pro Phe Met Gln His 705 710 715 720 Ile Tyr Glu Glu Gly Leu Asp Val Ser Ser Ile Gln Leu Leu Ile Thr 725 730 735 Ser Ser Asp Ala Cys Ser Val Thr Asp Tyr Arg Leu Leu Leu Glu Arg 740 745 750 Phe Gly Gly Gln Phe Arg Ile Ile Asn Ser Tyr Gly Val Thr Glu Ala 755 760 765 Ala Ile Asp Ser Ser Phe Tyr Gly Glu Pro Leu Asp Lys Leu Pro Pro 770 775 780 Ser Gly His Val Pro Ile Gly Lys Ala Trp Leu Asn Ala Arg Phe Tyr 785 790 795 800 Ile Val Asp Ala Ala Leu Lys Pro Val Pro Val Gly Val Pro Gly Glu 805 810 815 Leu Val Ile Gly Gly Ala Gly Val Ala Arg Gly Tyr Trp Asn Arg Leu 820 825 830 Asp Leu Thr Ala Glu Lys Phe Val Asp Ser Pro Phe Val Pro Gly Glu 835 840 845 Arg Val Tyr Arg Thr Gly Asp Leu Ala Arg Trp Leu Glu Asp Gly Asn 850 855 860 Val Asp Phe Ile Gly Arg Ile Asp Tyr Gln Val Lys Ile Arg Gly Phe 865 870 875 880 Arg Ile Glu Leu Gly Glu Ile Glu Thr Ala Leu Leu Arg Phe Pro Gly 885 890 895 Val Lys Gln Ala Val Val Thr Asp Arg Thr Asp Glu Gln Gly Gln Lys 900 905 910 Tyr Leu Cys Gly Tyr Val Ala Ala Asp Ala Ser Leu Gln Leu Ser Asp 915 920 925 Leu Leu Ser Gln Leu Lys Gln Glu Leu Pro Ala His Met Val Pro Ala 930 935 940 Arg Leu Val Ser Leu Asp Lys Leu Pro Leu Thr Pro Asn Gly Lys Ile 945 950 955 960 Asp Arg Lys Ala Leu Pro Glu Pro Thr Gly Glu Val Glu Thr Gly Gly 965 970 975 Glu Tyr Val Ala Pro Arg Thr Thr Leu Glu Thr Arg Leu Ala Leu Ile 980 985 990 Trp Gln Gln Val Leu Gly Ile Ala Arg Val Gly Val Gln Asp Asp Phe 995 1000 1005 Phe Asp Leu Gly Gly His Ser Leu Arg Ala Ser Thr Leu Val Ser Lys 1010 1015 1020 Ile Arg Lys Glu Leu Gln Val Glu Val Pro Leu Arg Glu Val Phe Arg 1025 1030 1035 1040 Tyr Thr Thr Ile Glu Gln Leu Ala Gln Arg Ile Gly Gly Leu Arg Gln 1045 1050 1055 Gln Glu Thr Tyr Glu Ile Thr Lys Ala Ala Glu Ala Glu Tyr Tyr Pro 1060 1065 1070 Val Ser Ser Glu Gln Lys Arg Leu Tyr Val Leu Arg Gln Leu Asp Gly 1075 1080 1085 Ala Glu Arg Ser Tyr Asn Met Ser Ala Ala Leu Leu Leu Glu Gly Lys 1090 1095 1100 Leu Asp Arg Thr Arg Val Glu His Ala Phe Arg Ala Leu Ile Gln Arg 1105 1110 1115 1120 His Glu Thr Leu Arg Thr Gly Ile Glu Gln Val Gln Gly Glu Leu Val 1125 1130 1135 Gln Arg Ile Tyr Asp Glu Val Glu Phe Ala Val Asp Tyr Phe Gln Ala 1140 1145 1150 Ser Glu Trp Glu Val Asp Gln Val Val Glu Ala Tyr Tyr His Pro Phe 1155 1160 1165 Asp Leu Thr Lys Pro Pro Leu Leu Arg Ile Gly Leu Ile Glu Val Asp 1170 1175 1180 Glu Asp Arg His Ile Leu Leu Phe Asp Met His His Ile Val Ser Asp 1185 1190 1195 1200 Gly Ile Ser Thr Ala Leu Leu Phe Asp Glu Phe Ser Arg Leu Tyr Arg 1205 1210 1215 Gly Glu Glu Leu Ala Pro Leu Arg Ile Gln Tyr Lys Asp Tyr Ala Val 1220 1225 1230 Trp Gln His Ser Glu Ala Tyr Gly Gln Met Leu Gln Pro Gln Lys Glu 1235 1240 1245 Tyr Trp Leu Lys Gln Leu Ser Gly Glu Leu Pro Val Leu Glu Leu Pro 1250 1255 1260 Thr Asp Phe Pro Arg Pro Ala Val Gln Ser Phe Asp Gly Arg Thr Val 1265 1270 1275 1280 Lys Phe Tyr Ile Glu Lys Asp Arg Thr Glu Lys Leu Lys Glu Leu Ala 1285 1290 1295 Ala Arg Thr Gly Thr Thr Leu Tyr Met Val Leu Leu Ser Ala Tyr Thr 1300 1305 1310 Ile Leu Met His Lys Tyr Ser Gly Gln Glu Asp Leu Ile Val Gly Thr 1315 1320 1325 Pro Ile Ala Gly Arg Thr Gln Glu Glu Val Gln Pro Ile Val Gly Met 1330 1335 1340 Phe Ile Asn Thr Leu Ala Ile Arg Ser Arg Pro Glu Arg Ser Lys Pro 1345 1350 1355 1360 Tyr Leu Ser Tyr Leu Glu Glu Ile Lys Asp Ile Thr Leu Gly Ala Phe 1365 1370 1375 Glu His Gln Asn Tyr Leu Phe Glu Asp Leu Val Glu Ser Leu His Ile 1380 1385 1390 Pro Arg Ala Thr Gly Arg Asn Pro Leu Phe Asp Thr Phe Phe Ser Leu 1395 1400 1405 Gln Asn Thr Glu Asn Glu Gln Ile Val Ile Glu Gly Leu Glu Gln Ser 1410 1415 1420 Phe Tyr Pro Leu Glu Asn Gln Thr Ser Lys Phe Glu Leu Leu Leu Asp 1425 1430 1435 1440 Ile Ser Glu Leu Asp Gly Gln Leu Glu Cys Arg Leu Glu Tyr Ala Thr 1445 1450 1455 Ala Leu Tyr Lys Gln Glu Thr Ala Glu Arg Phe Ala Arg His Tyr Asp 1460 1465 1470 Lys Leu Leu Glu Thr Ile Ala Ala Ala Pro Asp Gly Asp Ile Ala Ser 1475 1480 1485 Leu Glu Met Leu Thr Glu Glu Glu Ile Arg Glu Leu Val Arg Gly Phe 1490 1495 1500 Asn Asp Ser Glu Ala Asp Tyr Pro Arg Gln Gln Thr Ile His Gly Leu 1505 1510 1515 1520 Phe Glu Glu Gln Ala Glu Leu Tyr Pro Asp Asn Val Ala Ala Val Met 1525 1530 1535 Asn Glu Arg Gln Leu Thr Tyr Arg Glu Leu Asn Glu Arg Ser Asn Arg 1540 1545 1550 Leu Ala Arg Lys Leu Arg Glu Thr Gly Val Glu Ala Asp Gln Leu Val 1555 1560 1565 Ala Ile Leu Ala Glu Arg Ser Leu Asp Met Val Val Gly Ile Leu Ala 1570 1575 1580 Ile Leu Lys Ala Gly Gly Ala Tyr Val Pro Val Asp Pro Asp Tyr Pro 1585 1590 1595 1600 Glu Glu Arg Ile Arg Phe Met Ile Glu Asp Ser Gly Ala Pro Leu Leu 1605 1610 1615 Leu Ile Gln Lys His Leu His Glu Lys Thr Asp Phe Ala Gly Thr Arg 1620 1625 1630 Leu Glu Leu Asp Asp Phe Val Gly Gly Asp Arg Gly Ala Asn Ser Ala 1635 1640 1645 Asp Val Arg Asp Ala Ser Asn Leu Glu Pro Ile Ser Gly Pro Gly Asn 1650 1655 1660 Leu Ala Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly Arg Pro Lys Gly 1665 1670 1675 1680 Thr Leu Ile Glu His Lys Asn Val Val Arg Leu Leu Phe Asn Asp Lys 1685 1690 1695 Asn Leu Phe Asp Phe Gly Pro Ser Asp Thr Trp Thr Leu Phe His Ser 1700 1705 1710 Phe Cys Phe Asp Phe Ser Val Trp Glu Met Tyr Gly Ala Leu Leu Tyr 1715 1720 1725 Gly Gly Lys Leu Val Ile Val Pro Pro Leu Thr Ala Lys Ser Pro Ala 1730 1735 1740 Asp Phe Leu Ala Leu Leu Gly Arg Glu Gln Val Thr Ile Leu Asn Gln 1745 1750 1755 1760 Thr Pro Thr Tyr Phe Tyr Gln Leu Leu Arg Glu Val Leu Thr Asp His 1765 1770 1775 Pro Tyr Asp Leu Arg Ile Arg Asn Val Ile Phe Gly Gly Glu Ala Leu 1780 1785 1790 Ser Pro Leu Leu Leu Lys Gly Phe Lys Thr Lys Tyr Pro Glu Thr Lys 1795 1800 1805 Leu Ile Asn Met Tyr Gly Ile Thr Glu Thr Thr Val His Val Thr Tyr 1810 1815 1820 Lys Glu Ile Thr Trp Val Glu Met Glu Ala Ala Lys Ser Asn Ile Gly 1825 1830 1835 1840 Lys Pro Ile Pro Thr Leu Ser Val Tyr Val Leu Asp Glu Asn Arg Arg 1845 1850 1855 Pro Val Pro Ile Gly Val Ala Gly Glu Met Tyr Val Ala Gly Glu Gly 1860 1865 1870 Leu Ala Arg Gly Tyr Leu Asn Arg Pro Asp Leu Thr Ala Glu Lys Phe 1875 1880 1885 Val Asp Ser Pro Phe Ala Glu Gly Glu Lys Leu Tyr Arg Ser Gly Asp 1890 1895 1900 Leu Ala Ala Trp Leu Pro Asp Gly Asn Ile Glu Tyr Leu Gly Arg Ile 1905 1910 1915 1920 Asp His Gln Val Lys Ile Arg Gly Tyr Arg Ile Glu Leu Asp Glu Ile 1925 1930 1935 Glu Thr Gln Leu Leu Asn Val Glu Gly Val Glu Glu Ala Val Val Leu 1940 1945 1950 Ala Arg Gln Asp Gly Gly Gly Glu Lys Ala Leu Val Ala Tyr Phe Val 1955 1960 1965 Ala Asp Arg Thr Leu Thr Val Ser Glu Met Arg Thr Ser Leu Ala Gln 1970 1975 1980 Gly Met Pro Gly Tyr Met Ile Pro Ser Tyr Phe Leu Gln Leu Glu Arg 1985 1990 1995 2000 Met Pro Leu Thr Ser Asn Gly Lys Val Asp Arg Lys Ala Leu Pro Glu 2005 2010 2015 Pro Gln Gly Gly Leu Gln Thr Gly Val Glu Tyr Val Ala Pro Arg Asn 2020 2025 2030 Arg Thr Glu Ser Gln Leu Val Lys Ile Trp Glu Glu Val Leu Gly Tyr 2035 2040 2045 Ser Gly Ile Gly Val Leu Asp Asn Phe Phe Glu Leu Gly Gly His Ser 2050 2055 2060 Leu Arg Ala Thr Asn Leu Val Ser Lys Ile Arg Lys Glu Met Asn Val 2065 2070 2075 2080 Glu Leu Pro Leu Arg Asp Val Phe Arg Tyr Thr Thr Val Glu Ser Met 2085 2090 2095 Ala Gly Ala Ile Ala Ser Leu Glu Glu Thr Gln His Ser Ser Ile Pro 2100 2105 2110 Lys Ala Glu Glu Lys Ala Tyr Tyr Pro Val Ser Ser Ala Gln Lys Arg 2115 2120 2125 Leu Tyr Val Leu His Gln Leu Asp Ser Ser Glu Leu Asn Tyr Asn Leu 2130 2135 2140 Pro Ser Ala Leu Gln Leu Glu Gly Ala Leu Asn Glu Ala Lys Val Glu 2145 2150 2155 2160 Lys Ala Leu Thr Thr Leu Val Ala Arg His Asp Met Leu Arg Thr Gly 2165 2170 2175 Phe Glu Ile Val Asn Gly Glu Pro Val Gln Arg Ile His Pro Leu Val 2180 2185 2190 Ala Phe Lys Val Glu Lys Leu Gln Ala Ser Glu Asp Gln Val Ala Ala 2195 2200 2205 Ile Leu Glu Gly Phe Ile Gln Pro Phe Asp Leu Thr Gln Pro Pro Leu 2210 2215 2220 Leu Arg Ala Leu Leu Ile Glu Leu Glu Lys Glu Lys Phe Leu Leu Ala 2225 2230 2235 2240 Leu Asp Ile His His Ile Gly Ser Asp Gly Leu Ser Met Asp Val Leu 2245 2250 2255 Leu Arg Glu Phe Val Arg Leu Tyr Asn Gly Glu Glu Leu Pro Glu Leu 2260 2265 2270 Arg Ile Gln Tyr Lys Asp Tyr Ala Val Trp Gln Gln Ser Glu Glu Gln 2275 2280 2285 Arg Gln Arg Ile Lys Arg Gln Glu Glu Tyr Trp Arg Gly Val Phe Ser 2290 2295 2300 Ser Glu Leu Pro Val Leu Glu Leu Pro Leu Asp Phe Ser Arg Pro Ala 2305 2310 2315 2320 Val Gln Gln Phe Asp Gly Gln Thr Leu Thr Phe Thr Leu Asp Ala Glu 2325 2330 2335 Lys Ser Glu Ala Leu Lys Arg Leu Ala Gly Asp Ser Gly Ala Thr Leu 2340 2345 2350 Tyr Met Leu Leu Leu Ala Ala Tyr Ser Val Leu Leu His Lys Tyr Ala 2355 2360 2365 Gly Gln Glu Asp Ile Val Val Gly Thr Pro Ile Ala Ala Arg Ser His 2370 2375 2380 Ala Asp Leu Gln Pro Ile Ile Gly Met Phe Val Asn Thr Leu Ala Leu 2385 2390 2395 2400 Arg Leu Gly Pro Val Ala Glu Arg Thr Phe Leu Asp Tyr Leu Gln Glu 2405 2410 2415 Val Lys Glu Thr Thr Leu Gly Ala Tyr Glu His Gln Asp Tyr Pro Phe 2420 2425 2430 Glu Glu Leu Val Glu Ala Leu Gln Val Ser Arg Asp Leu Ser Arg Asn 2435 2440 2445 Pro Leu Phe Asp Thr Met Phe Ser Leu Gln Lys His Glu Ser Leu Asp 2450 2455 2460 Leu Thr Leu Glu Gly Leu Gln Trp Ser Leu Phe Asp Ile Glu Glu Lys 2465 2470 2475 2480 Thr Ala Lys Phe Asp Leu Ser Phe Asp Ile Val Glu Ala Asp Asn Glu 2485 2490 2495 Leu Ile Cys Lys Ile Glu Tyr Ala Thr Ser Leu Phe Arg Gln Glu Thr 2500 2505 2510 Met Val Arg Leu Ala Gly His Tyr Glu Gln Leu Leu Ala Ser Ile Leu 2515 2520 2525 Ala Gln Pro Gly Ala Arg Ile Ser Asp Leu Asp Ile Leu Thr Asp Ser 2530 2535 2540 Glu Lys His Asp Leu Leu Val Gly Phe Asp Val Ser Ser Ser Ala Leu 2545 2550 2555 2560 Ala Lys Gln Pro Ala Ala Glu Gly Thr Gly Leu Glu Ala Asp Glu Ser 2565 2570 2575 Trp Arg Glu Arg Thr Phe His Glu Leu Phe Glu Glu Gln Ala Glu Arg 2580 2585 2590 Thr Pro Gly Ala Leu Ala Val Val Tyr Glu Asp Ser Lys Leu Thr Tyr 2595 2600 2605 Ala Glu Leu Asn Ala Lys Ala Asn Arg Leu Ala Tyr Ala Leu Arg Ala 2610 2615 2620 Arg Gly Val Lys Pro Glu Gln Val Val Gly Ile Leu Ala Gly Arg Ser 2625 2630 2635 2640 Ala Glu Leu Leu Ile Gly Val Leu Ala Val Trp Lys Ala Gly Gly Ala 2645 2650 2655 Tyr Val Pro Leu Asp Pro Asp Tyr Pro Ala Glu Arg Ile Glu Tyr Met 2660 2665 2670 Leu Ala Asp Ser Gly Ala Ser Val Leu Leu Thr Gln Thr Arg Leu Leu 2675 2680 2685 Glu Gln Ala Glu Ala Trp Arg Ser Asp Gly Ala Leu Val Leu Gln Thr 2690 2695 2700 Val Leu Ala Leu Asp Asp Ala Ala Thr Tyr Ser Leu Gly Ala Ala Glu 2705 2710 2715 2720 Ala Ala Val Gly Glu Ala Gly Ala Glu Ala Glu Ala Leu Ala Gln Ala 2725 2730 2735 Ala Ala Ala Glu Ser Ser Ala Thr Ala Glu Ala Glu Lys Asn Val Leu 2740 2745 2750 Ala Ala Asp Leu Ala Ser Asn Pro Ala Asn Val Asn Lys Pro Arg Asp 2755 2760 2765 Leu Ala Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly Arg Pro Lys Gly 2770 2775 2780 Val Ala Val Glu Asn Arg Ser Leu Val Asn Thr Ala Ala Gly Tyr Arg 2785 2790 2795 2800 Arg Glu Tyr Arg Leu Asp Gln Phe Pro Val Arg Leu Leu Gln Leu Ala 2805 2810 2815 Ser Phe Ser Phe Asp Val Phe Val Gly Asp Ile Ala Arg Thr Leu Tyr 2820 2825 2830 Asn Gly Gly Thr Met Val Ile Val Pro Lys Asp Asp Arg Ile Asp Pro 2835 2840 2845 Thr Arg Leu Tyr Gly Trp Ile Arg Asp Tyr Ala Val Thr Val Phe Glu 2850 2855 2860 Ser Thr Pro Ala Leu Ile Val Pro Phe Met Glu His Val His Ala Glu 2865 2870 2875 2880 Gly Leu Asp Leu Ser Ser Met Gln Leu Leu Ile Thr Ser Ser Asp Ala 2885 2890 2895 Cys Ser Val Ala Asp Tyr Arg Thr Leu Gln Glu Arg Phe Gly Ser Gln 2900 2905 2910 Phe Arg Ile Ile Asn Ser Tyr Gly Val Thr Glu Ala Ala Ile Asp Ser 2915 2920 2925 Ser Phe Tyr Asp Glu Pro Leu Glu Lys Leu Pro Lys Thr Gly Ser Val 2930 2935 2940 Pro Ile Gly Lys Ala Trp Leu Asn Ala Lys Phe Tyr Ile Val Asp Ala 2945 2950 2955 2960 Asn Leu Lys Pro Val Pro Ile Gly Val Leu Gly Glu Leu Val Ile Gly 2965 2970 2975 Gly Ala Gly Val Ala Arg Gly Tyr Leu Asn Arg Pro Asp Leu Thr Ala 2980 2985 2990 Glu Lys Phe Val Asp Ser Pro Phe Ala Ala Gly Glu Arg Leu Tyr Arg 2995 3000 3005 Thr Gly Asp Leu Ala Arg Trp Met Pro Asp Gly Asn Val Asp Phe Ile 3010 3015 3020 Gly Arg Ile Asp Asn Gln Val Lys Ile Arg Gly Tyr Arg Ile Glu Leu 3025 3030 3035 3040 Gly Glu Ile Glu Ala Ala Met Lys Asn Phe Ala Gly Val Arg Gln Ala 3045 3050 3055 Leu Val Ile Asp Arg Thr Asp Glu Arg Gly Gln Lys Tyr Leu Cys Gly 3060 3065 3070 Tyr Val Val Ala Asp Ser Ser Phe Asp Leu Glu Gly Leu Val Ala His 3075 3080 3085 Leu Asp Ala Ala Leu Pro Ser His Met Val Pro Ser Arg Ile Met Arg 3090 3095 3100 Leu Asp Gln Met Pro Leu Thr Pro Asn Gly Lys Ile Asp Arg Lys Ala 3105 3110 3115 3120 Leu Pro Val Pro Glu Gly Ser Ile Arg Ala Glu Ala Ala Tyr Thr Ala 3125 3130 3135 Pro Arg Thr Pro Ala Glu Gln Ala Leu Ala Ser Val Trp Gln Ser Val 3140 3145 3150 Leu Gly Val Asp Gln Val Gly Thr Met Asp Asn Phe Phe Ala Leu Gly 3155 3160 3165 Gly Asp Ser Ile Lys Ala Leu Gln Val Ser Ser Arg Leu Leu Gln Thr 3170 3175 3180 Gly Tyr Lys Leu Val Met Lys Asp Leu Phe His Tyr Pro Thr Ile Ser 3185 3190 3195 3200 Ala Leu Ser Leu Gln Leu Gln Thr Ala Glu Arg Thr Ala Ser Gln Ala 3205 3210 3215 Glu Val Thr Gly Glu Val Ile Leu Thr Pro Ile Gln Arg Trp Phe Phe 3220 3225 3230 Glu Gln Asn Pro Ala Asp Val His His Ser Asn Gln Ala Phe Met Gln 3235 3240 3245 Phe Ser Lys Gln Gly Phe Asp Glu Glu Ala Leu Arg Gln Ala Val Arg 3250 3255 3260 Gln Leu Val Val His His Asp Ala Leu Arg Thr Val Tyr Arg Gln Thr 3265 3270 3275 3280 Glu Asn Gly Tyr Thr Ala Trp Asn Arg Gly Ala Gly Glu Asn Glu Ala 3285 3290 3295 Leu Phe Asp Leu Glu Val Val Asp Phe Lys Gly Val Gly Asp Val Lys 3300 3305 3310 Glu Ala Val Glu Ala Lys Ala Asn Asp Ile Gln Ala Ser Ile Asp Leu 3315 3320 3325 Glu Asn Gly Pro Leu Val Lys Leu Gly Leu Phe Arg Cys Asp Asp Gly 3330 3335 3340 Asp His Leu Leu Ile Ala Ile His His Leu Val Val Asp Gly Val Ser 3345 3350 3355 3360 Trp Arg Ile Leu Leu Glu Asp Phe Ala Ala Cys Tyr Glu Gln Ala Leu 3365 3370 3375 Gln Gly Gln Pro Ile Arg Leu Pro Leu Lys Thr Asp Ser Phe Gln Thr 3380 3385 3390 Trp Ala Lys Gln Leu Ala Asp Tyr Ala Asn Gly Pro Ala Met Glu Ser 3395 3400 3405 Glu Arg Glu Tyr Trp Gln His Ile Glu Gln Leu Thr Tyr Glu Pro Leu 3410 3415 3420 Pro Lys Asp Phe Glu Gln Gly Arg Ser Lys Leu Lys Asp Ser Gly Leu 3425 3430 3435 3440 Val Thr Val Arg Trp Thr Ala Glu Glu Thr Glu Gln Leu Leu Lys Gln 3445 3450 3455 Ala His Cys Ala Tyr His Thr Glu Met Asn Asp Leu Leu Leu Ala Ala 3460 3465 3470 Leu Gly Leu Ala Val Gln Ala Trp Ser Gly Arg Glu Arg Val Leu Val 3475 3480 3485 Asn Leu Glu Gly His Gly Arg Glu Asp Ile Leu Pro Asn Val Asp Ile 3490 3495 3500 Thr Arg Thr Val Gly Trp Phe Thr Ser Gln Phe Pro Val Val Leu Glu 3505 3510 3515 3520 Pro Gly His Ala Gln Ala Leu Gly His Gln Val Lys Gln Val Lys Glu 3525 3530 3535 Ser Leu Arg Arg Ile Pro Asn Lys Gly Ile Gly Tyr Gly Ile Leu Arg 3540 3545 3550 Tyr Leu Ser Ala Pro Arg Asp Gly Glu Arg Phe Ala Leu Glu Pro Glu 3555 3560 3565 Ile Ser Phe Asn Tyr Leu Gly Gln Phe Asp Gln Asp Tyr Glu Ser Ser 3570 3575 3580 Gly Ser Arg Pro Ser Pro Phe Ser Pro Gly Ser Asp Ser Ser Pro Asp 3585 3590 3595 3600 Ala Val Met Asp Tyr Val Leu Asp Ile Asn Gly Met Val Ser Glu Gly 3605 3610 3615 Val Gln Glu Leu Thr Ile Arg Tyr Gly Glu Thr Gln Tyr Lys Arg Glu 3620 3625 3630 Thr Val Glu Arg Leu Gly Thr Leu Leu His Ser Ser Leu Arg Glu Val 3635 3640 3645 Ile Ser His Cys Val Ser Lys Glu Arg Pro Glu Leu Thr Pro Ser Asp 3650 3655 3660 Val Leu Leu Gln Asp Val Thr Leu Glu Glu Leu Glu Arg Leu Ala Glu 3665 3670 3675 3680 His Thr Ala Ala Leu Gly Glu Leu Glu Asn Val Tyr Thr Leu Thr Pro 3685 3690 3695 Leu Gln Lys Gly Met Leu Phe His Ser Leu Leu Asp Ala Asp Ser Glu 3700 3705 3710 Ala Tyr Phe Glu Gln Val Thr Phe Asp Leu Tyr Gly Ser Leu Asn Val 3715 3720 3725 Glu Ala Phe Thr Gln Gly Leu Asp Thr Leu Val Gln Arg Asn Glu Ala 3730 3735 3740 Leu Arg Thr Asn Phe Ile Thr Gly Trp Arg Asp Glu Pro Ile Gln Val 3745 3750 3755 3760 Val Phe Arg Glu Arg Lys Cys Glu Val Tyr Phe Glu Asp Ile Arg Ser 3765 3770 3775 Val Ser Asp Glu Asp Pro Glu Lys Thr Ile Ala Asp Phe Val Ser Ala 3780 3785 3790 Asp Lys Ala Asn Lys Phe Asp Leu Ala Arg Gly Ser Leu Met Arg Val 3795 3800 3805 Thr Val Leu Arg Thr Gly Asp Glu Ser Tyr His Val Ile Trp Ser His 3810 3815 3820 His His Ile Leu Met Asp Gly Trp Cys Met Ser Phe Met Ile Lys Glu 3825 3830 3835 3840 Val Phe Asp Thr Tyr Phe Ala Phe Gln Glu Lys Arg Thr Leu Glu Leu 3845 3850 3855 Pro Pro Val Thr Ser Tyr Ser Arg Tyr Ile Glu Trp Leu Glu Ala Gln 3860 3865 3870 Asp Ala Ala Lys Ala Ser Arg Tyr Trp Ser Glu Tyr Leu Ala Gly Tyr 3875 3880 3885 Asp Gln Gln Thr Lys Leu Pro Gln Glu Lys Thr Gln Leu Lys Gln Gly 3890 3895 3900 Ala Phe Glu Ala Ala Glu Ile Asp Val Glu Leu Ser Lys Glu Leu Thr 3905 3910 3915 3920 Gly Gln Ile Glu Arg Val Ala Arg Gln Gln Gln Val Thr Leu Asn Thr 3925 3930 3935 Phe Met Gln Thr Val Trp Gly Leu Val Leu Gln Val Tyr Asn Asn Ser 3940 3945 3950 Glu Asp Val Val Phe Gly Ser Val Val Ser Gly Arg Pro Ala Glu Ile 3955 3960 3965 Pro Gly Ile Glu Ser Met Ile Gly Leu Phe Ile Asn Thr Ile Pro Val 3970 3975 3980 Arg Ile Gln Gly Lys Ala Glu Glu Thr Val Ala Asp Ile Leu Arg Lys 3985 3990 3995 4000 Thr Gln Asp Gln Ala Leu Ala Ser Gly Ala Tyr Glu Thr Phe Pro Leu 4005 4010 4015 Phe Glu Ile Gln Ser Leu Ser Glu His Lys Arg Asp Leu Ile Asn His 4020 4025 4030 Ile Met Val Phe Glu Asn Tyr Pro Met Glu Glu Gln Ile Glu Gln Val 4035 4040 4045 Val Gly Gly Asp Lys Glu Ala Leu Lys Ile Ala Asn Ile Gln Ser Pro 4050 4055 4060 Glu Gln Thr Asn Tyr Asp Leu Asp Ile Thr Val Ile Pro Glu Glu His 4065 4070 4075 4080 Ile Leu Leu Arg Phe Thr Tyr Asn Ala Leu Thr Phe Arg Glu Glu Asp 4085 4090 4095 Ile Arg Leu Ile His Gly His Phe Ala Arg Val Leu Glu Lys Val Ala 4100 4105 4110 Ala Asn Pro Ser Ile Arg Val Asn Gln Leu Glu Leu Leu Thr Ala Ala 4115 4120 4125 Glu Lys Glu Gln Ile Leu Gly Ala Phe Asn Pro Ala Gln Pro Glu Ala 4130 4135 4140 Ala Pro Ala Ala Ala Phe His Arg Leu Phe Glu Glu Gln Ala Glu Arg 4145 4150 4155 4160 Thr Pro Glu Ala Ala Ala Val Val Tyr Glu Asn Asp Arg Leu Thr Tyr 4165 4170 4175 Glu Glu Leu Asn Glu Arg Ala Asn Arg Leu Ala Ala Thr Leu Arg Ala 4180 4185 4190 Ser Gly Ile Gly Arg Glu Thr Ile Val Gly Ile Leu Ala Glu Arg Ser 4195 4200 4205 Val Asp Leu Leu Val Ala Val Leu Ala Val Trp Lys Ala Gly Gly Ala 4210 4215 4220 Tyr Val Pro Leu Asp Pro Asp Tyr Pro Ala Glu Arg Val Arg Phe Met 4225 4230 4235 4240 Leu Glu Asp Ser Gly Ala Lys Val Leu Leu Thr Gln Thr Pro Leu Arg 4245 4250 4255 Glu Arg Ala Glu Ala Trp Leu Gly Glu Glu Glu Leu Ala Leu Ala Ala 4260 4265 4270 Val Leu Tyr Leu Asp Asp Glu Thr Ser Tyr Ser Glu Glu Arg Thr Asn 4275 4280 4285 Ala Asp Ser Phe His Glu Ala Arg Pro Glu Asp Leu Ala Tyr Val Ile 4290 4295 4300 Tyr Thr Ser Gly Thr Thr Gly Lys Pro Lys Gly Val Met Ile Glu His 4305 4310 4315 4320 Arg Ser Leu Val Asn Thr Ala Ala Gly Tyr Arg Arg Glu Tyr Arg Leu 4325 4330 4335 Asp Gln Phe Pro Val Arg Leu Leu Gln Leu Ala Ser Phe Ser Phe Asp 4340 4345 4350 Val Phe Val Gly Asp Ile Ala Arg Thr Leu Tyr Asn Gly Gly Thr Met 4355 4360 4365 Val Ile Val Pro Lys Asp Asp Arg Ile Asp Pro Ser Arg Leu His His 4370 4375 4380 Trp Met Glu Arg Glu Arg Val Thr Ile Phe Glu Ser Thr Pro Ala Leu 4385 4390 4395 4400 Ile Val Pro Phe Leu Glu Tyr Val His Asp Gln Arg Leu Asp Met Ser 4405 4410 4415 Trp Met Glu Leu Leu Ile Thr Ser Ser Asp Ser Cys Ser Val Ala Asp 4420 4425 4430 Tyr Arg Thr Leu Gln Glu Arg Phe Gly Ser Leu Phe Arg Ile Ile Asn 4435 4440 4445 Ala Tyr Gly Val Thr Glu Ala Ala Ile Asp Ser Ser Phe Tyr Asp Glu 4450 4455 4460 Glu Leu Ala Lys Leu Pro Gln Thr Gly Asn Val Pro Ile Gly Lys Ala 4465 4470 4475 4480 Trp Leu Asn Ala Lys Phe Tyr Ile Val Asp Ala His Leu Asn Pro Val 4485 4490 4495 Pro Val Gly Val Leu Gly Glu Leu Val Ile Gly Gly Val Gly Val Ala 4500 4505 4510 Arg Gly Tyr Leu Asn Arg Pro Glu Leu Thr Glu Glu Lys Phe Val Asp 4515 4520 4525 Ser Pro Phe Ala Ala Gly Glu Arg Leu Tyr Arg Thr Gly Asp Leu Ala 4530 4535 4540 Arg Trp Met Glu Asp Gly Asn Val Asp Phe Ile Gly Arg Ile Asp Asn 4545 4550 4555 4560 Gln Ala Lys Ile Arg Gly Tyr Arg Ile Glu Thr Gly Glu Val Glu Ala 4565 4570 4575 Lys Leu Leu Ser Val Gly Gly Val Lys Glu Ala Val Val Val Val Arg 4580 4585 4590 Glu Asp Gln Glu Gly Gln Lys Ala Leu Cys Ala Tyr Tyr Thr Ala Glu 4595 4600 4605 Glu Gly Leu Thr Ala Ala Asp Leu Lys Arg Ala Ile Ala Asn Glu Leu 4610 4615 4620 Pro Gly Tyr Met Ile Pro Ser Tyr Phe Val Glu Leu Glu His Leu Pro 4625 4630 4635 4640 Leu Thr Pro Asn Gly Lys Ile Asp Arg Lys Ala Leu Pro Ala Pro Glu 4645 4650 4655 Gly Gly Ala Ser Ala Gly Arg Glu Tyr Val Ala Pro Arg Thr Glu Leu 4660 4665 4670 Glu Ala Lys Leu Ala Ala Ile Trp Gln Asp Val Leu Val Arg Glu Lys 4675 4680 4685 Ala Val Gly Val Thr Asp Asn Phe Phe Asp Leu Gly Gly His Ser Leu 4690 4695 4700 Arg Ala Thr Thr Leu Val Ser Lys Met His Lys Glu Leu Gly Val Glu 4705 4710 4715 4720 Phe Pro Leu Arg Asp Val Phe Arg Tyr Ser Thr Val Glu Glu Met Ala 4725 4730 4735 Ala Ala Met Glu Arg Leu Glu Ile Gly Ser Phe Thr Ala Ile Pro Ala 4740 4745 4750 Ala Glu Pro Ser Glu Tyr Tyr Pro Leu Ser Ser Ala Gln Lys Arg Leu 4755 4760 4765 Tyr Ile Leu Asn Gln Leu Glu Gly Ala Glu Leu Ser Tyr Asn Ile Pro 4770 4775 4780 Gly Ala Met Leu Leu Glu Gly Glu Leu Asp Arg Gln Arg Phe Glu Glu 4785 4790 4795 4800 Ala Phe Arg Gly Leu Val Ala Arg His Glu Thr Leu Arg Thr Gly Phe 4805 4810 4815 Glu Met Val Lys Gly Glu Ala Val Gln Arg Ile Tyr Glu Glu Ala Ala 4820 4825 4830 Phe Gln Val Glu Tyr Val Gln Ile Ser Ala Glu Gln Ala Glu Glu Thr 4835 4840 4845 Val Arg Gln Phe Val Arg Pro Phe Asp Leu Ala Lys Pro Pro Leu Leu 4850 4855 4860 Arg Val Gly Leu Ala Glu Leu Ala Pro Asp Arg His Ile Leu Met Phe 4865 4870 4875 4880 Asp Thr His His Ile Val Ser Asp Gly Val Ser Ile Asp Val Leu Ile 4885 4890 4895 Glu Glu Phe Val Arg Leu Tyr Ser Gly Glu Gln Leu Glu Pro Leu Arg 4900 4905 4910 Ile Gln Tyr Lys Asp Tyr Ala Val Trp Gln Gln Ser Asp Glu Gln Lys 4915 4920 4925 Ala Gln Leu Ala Lys Gln Glu Ala Tyr Trp Leu Asp Met Phe Arg Gly 4930 4935 4940 Glu Leu Pro Val Leu Glu Leu Pro Met Asp Tyr Pro Arg Pro Ala Met 4945 4950 4955 4960 Gln Ser Tyr Glu Gly Arg Thr Leu Gln Leu Phe Met His Ser Glu Lys 4965 4970 4975 Ser Glu Gly Leu Lys Arg Leu Ala Ala Glu Asn Gly Ala Thr Leu Tyr 4980 4985 4990 Met Val Leu Leu Ala Gly Tyr Thr Ile Leu Leu His Lys Tyr Thr Gly 4995 5000 5005 Gln Glu Asp Val Val Val Gly Thr Pro Ile Ala Gly Arg Asn His Ser 5010 5015 5020 Asp Val Gln Pro Leu Ile Gly Met Phe Val Asn Thr Leu Ala Ile Arg 5025 5030 5035 5040 Ser Tyr Pro Ala Ala Gly Lys Thr Phe Leu Asp Tyr Leu Lys Glu Ile 5045 5050 5055 Lys Glu Thr Thr Leu Gly Ala Phe Glu His Gln Asn Tyr Pro Phe Glu 5060 5065 5070 Glu Leu Val Asp Lys Val Asn Val Ala Arg Asp Leu Ser Arg Asn Pro 5075 5080 5085 Leu Phe Asp Thr Met Phe Ala Leu Gln Asn Thr Glu Asn Leu Glu Ile 5090 5095 5100 Gln Leu Pro Gly Leu His Leu Ser Thr Tyr Ala Ser Glu Glu Ile Val 5105 5110 5115 5120 Ser Lys Phe Asp Leu Ser Leu Asp Val Thr Glu Ile Glu Glu Gly Leu 5125 5130 5135 Glu Tyr Leu Phe Glu Tyr Ala Thr Ala Leu Tyr Lys Thr Glu Thr Val 5140 5145 5150 Glu Lys Leu Ala Ala His Tyr Leu Gln Leu Leu Glu Ser Ile Leu Arg 5155 5160 5165 Asn Pro Ser Ala Thr Ile Ala Glu Leu Gly Ile Leu Thr Pro Ala Glu 5170 5175 5180 Lys Glu Gln Ile Leu Gly Ala Phe Asn Pro Ala Gln Pro Gly Ala Ala 5185 5190 5195 5200 Pro Ala Ala Ala Phe His Arg Leu Phe Glu Glu Gln Ala Glu Arg Thr 5205 5210 5215 Pro Glu Glu Ala Ala Val Val Tyr Glu Asn Asp Arg Leu Thr Tyr Ala 5220 5225 5230 Glu Leu Asn Glu Arg Ala Asn Arg Leu Ala Ala Thr Leu Arg Ala Ser 5235 5240 5245 Gly Ile Gly Arg Glu Thr Ile Val Gly Ile Leu Ala Glu Arg Ser Val 5250 5255 5260 Asp Leu Leu Val Ala Val Leu Ala Val Trp Lys Ala Gly Gly Ala Tyr 5265 5270 5275 5280 Val Pro Leu Asp Pro Asp Tyr Pro Ala Asp Arg Val Arg Phe Met Leu 5285 5290 5295 Glu Asp Ser Gly Ala Lys Val Leu Leu Thr Gln Thr Pro Leu Arg Glu 5300 5305 5310 Arg Ala Glu Ala Trp Leu Asp Glu Glu Glu Leu Ala Leu Ala Ala Val 5315 5320 5325 Leu Tyr Leu Asp Asp Glu Ala Ser Tyr Ser Glu Glu Arg Ala Asn Ala 5330 5335 5340 Ser Val Val Ser Gly Ile Val Ser Gly Ile Val Ser Gly Lys Leu Thr 5345 5350 5355 5360 Asp Ala Val Asp Asp Gly Asp Glu Ser His Gln Asn Val Gly Thr Asp 5365 5370 5375 Ser Phe His Glu Ala Arg Pro Glu Asp Leu Ala Tyr Val Ile Tyr Thr 5380 5385 5390 Ser Gly Thr Thr Gly Lys Pro Lys Gly Val Met Ile Glu His Arg Ser 5395 5400 5405 Leu Val Asn Thr Ala Ala Gly Tyr Arg Arg Glu Tyr Arg Leu Asp Gln 5410 5415 5420 Phe Pro Val Arg Leu Leu Gln Leu Ala Ser Phe Ser Phe Asp Val Phe 5425 5430 5435 5440 Val Gly Asp Ile Ala Arg Thr Leu Tyr Asn Gly Gly Thr Met Val Ile 5445 5450 5455 Val Pro Lys Asp Asp Arg Ile Asp Pro Ser Arg Leu His His Trp Met 5460 5465 5470 Glu Arg Glu Arg Val Thr Ile Phe Glu Ser Thr Pro Ala Leu Ile Val 5475 5480 5485 Pro Phe Leu Glu Tyr Val His Glu Gln Gln Leu Asp Met Ser Trp Met 5490 5495 5500 Glu Leu Leu Ile Thr Ser Ser Asp Ser Cys Ser Val Ala Asp Tyr Arg 5505 5510 5515 5520 Thr Leu Gln Glu Arg Phe Gly Ser Leu Phe Arg Ile Ile Asn Ala Tyr 5525 5530 5535 Gly Val Thr Glu Ala Ala Ile Asp Ser Ser Phe Tyr Asp Glu Glu Leu 5540 5545 5550 Ala Lys Leu Pro Gln Thr Gly Asn Val Pro Ile Gly Lys Ala Trp Leu 5555 5560 5565 Asn Ala Lys Phe Tyr Ile Val Asp Ala His Leu Asn Pro Val Pro Val 5570 5575 5580 Gly Val Leu Gly Glu Leu Ala Ile Gly Gly Val Gly Val Ala Arg Gly 5585 5590 5595 5600 Tyr Leu Asn Arg Pro Glu Leu Thr Glu Glu Lys Phe Val Asp Ser Pro 5605 5610 5615 Phe Ala Ala Gly Glu Arg Leu Tyr Arg Thr Gly Asp Leu Ala Arg Trp 5620 5625 5630 Met Glu Asp Gly Asn Val Asp Phe Ile Gly Arg Ile Asp Asn Gln Ala 5635 5640 5645 Lys Ile Arg Gly Tyr Arg Ile Glu Thr Gly Glu Val Glu Ala Lys Leu 5650 5655 5660 Leu Ser Val Gly Gly Val Lys Glu Ala Val Val Val Val Arg Glu Asp 5665 5670 5675 5680 Gln Glu Gly Gln Lys Ala Leu Cys Ala Tyr Tyr Thr Ala Glu Glu Gly 5685 5690 5695 Leu Thr Ala Ala Asp Leu Lys Arg Ala Ile Ala Ser Glu Leu Pro Gly 5700 5705 5710 Tyr Met Ile Pro Ser Tyr Phe Val Glu Leu Glu Arg Leu Pro Leu Thr 5715 5720 5725 Pro Asn Gly Lys Ile Asp Arg Lys Ala Leu Pro Ala Pro Glu Gly Gly 5730 5735 5740 Ala Ser Ala Gly Arg Glu Tyr Val Ala Pro Arg Thr Glu Leu Glu Ala 5745 5750 5755 5760 Lys Leu Val Ala Ile Trp Gln Asp Val Leu Gly Pro Val Thr Ile Gly 5765 5770 5775 Val Thr Asp Asn Phe Phe Asp Leu Gly Gly His Ser Leu Arg Ala Thr 5780 5785 5790 Thr Leu Val Ser Lys Val His Lys Glu Leu Ser Val Asp Leu Pro Leu 5795 5800 5805 Arg Asp Val Phe Arg His Ser Thr Ile Glu Ala Met Ala Glu Ala Ile 5810 5815 5820 Ser Gln Leu Glu Arg Gln Glu His Leu Ser Ile Pro Val Leu Asp Lys 5825 5830 5835 5840 Arg Asp Tyr Tyr Pro Leu Ser Ser Val Gln Lys Arg Leu Tyr Ile Gln 5845 5850 5855 Gln Gln Met Glu Gly Ala Glu Leu Ser Tyr Asn Met Ser Gly Met Thr 5860 5865 5870 Val Leu Val Gly Arg Leu Glu Arg Asn Gln Phe Glu Ala Ala Leu Lys 5875 5880 5885 Gly Leu Ile Ala Arg His Glu Ile Leu Arg Thr Gly Phe Glu Met Val 5890 5895 5900 Asp Gly Glu Pro Val Gln Arg Ile Tyr Pro Asp Leu Lys Phe Ala Val 5905 5910 5915 5920 Glu Tyr Thr Lys Ala Thr Glu Ser Glu Thr Lys Ser Ile Val Asp Gly 5925 5930 5935 Phe Val Arg Val Phe Asp Leu Glu Arg Pro Pro Leu Leu Arg Val Gly 5940 5945 5950 Leu Val Glu Trp Glu Ala Glu Arg His Leu Leu Met Leu Asp Ile His 5955 5960 5965 His Ile Val Thr Asp Gly Met Ser Met Gly Ile Phe Val Glu Glu Leu 5970 5975 5980 Leu Arg Leu Tyr Asn Gly Glu Thr Met Glu Pro Leu Arg Ile Gln Tyr 5985 5990 5995 6000 Lys Glu Phe Ala Ala Trp Gln Gln Ser Glu Pro Val Lys Glu Arg Leu 6005 6010 6015 Lys Arg Gln Glu Ala Tyr Trp Leu Asp Val Leu Glu Gly Glu Leu Pro 6020 6025 6030 Thr Leu Glu Leu Pro Thr Asp Phe Val Arg Pro Ala Ala Arg Ser Phe 6035 6040 6045 Glu Gly Asp Val Leu Pro Phe Ser Ile Asp Lys Gln Met Thr Asp Ser 6050 6055 6060 Leu Gln Arg Ile Ala Asp Glu Asn Gly Ala Thr Leu Tyr Met Val Leu 6065 6070 6075 6080 Leu Ala Ala Tyr Ser Ile Leu Leu Ser Lys Tyr Ser Gly Gln Glu Asp 6085 6090 6095 Phe Ile Val Gly Thr Pro Val Ser Gly Arg Thr His Ala Asp Leu Glu 6100 6105 6110 Pro Leu Ile Gly Met Phe Val Asn Thr Leu Ala Ile Arg His Tyr Pro 6115 6120 6125 Ser Gly Glu Lys Thr Phe Leu Ala Tyr Leu Asn Glu Val Lys Glu Thr 6130 6135 6140 Met Leu Gly Ala Tyr Asp His Gln Asp Tyr Pro Phe Glu Glu Leu Val 6145 6150 6155 6160 Lys Lys Leu Gln Ala Pro Arg Asp Gln Gly Arg Asn Pro Val Phe Asp 6165 6170 6175 Val Met Phe Ala Leu Glu Thr Lys Glu Asp Asn Val Gln Ser Phe Gly 6180 6185 6190 Asp Ile Lys Ile Glu Ser Tyr Pro Glu Thr His Thr Val Ser Gln Phe 6195 6200 6205 Asp Leu Thr Leu Val Ile Ser Leu Leu Asp Glu Gly Met Asn Gly Gln 6210 6215 6220 Phe Glu Tyr Ala Thr Lys Leu Phe Thr Arg Asn Leu Ile Asp Asn Phe 6225 6230 6235 6240 Ala Gln Asp Leu Leu Val Ile Ile Thr Gln Ile Cys Glu Gln Pro Ser 6245 6250 6255 Val Leu Leu Lys Asp Ile Ser Leu Asn Gly Gln Ser Glu Gln Glu Gln 6260 6265 6270 Asp Val Leu Glu Thr Ile Asp Ile Ile Phe 6275 6280 <210> 4 <211> 4966 <212> PRT <213> Paenibacillus polymyxa ATCC21830 <400> 4 Met Ala Phe Glu Lys Glu Thr Leu Phe Trp Asn Glu Lys Phe Gly Ser 1 5 10 15 Asp Asp Tyr Thr Leu Thr Arg Leu Pro Tyr Ser Lys Ala Pro Ser Ser 20 25 30 Leu Thr Pro Ile Met Thr Thr Val Gly Gly Thr Leu Ser Glu Glu Val 35 40 45 Ala Gln Arg Val Leu Gln Met Ser Lys Gly Ala Pro Leu Ala Thr Phe 50 55 60 Met Ile Leu Leu Ala Gly Val Gln Ser Leu Leu Tyr Lys Tyr Thr Gly 65 70 75 80 Ala Ser Asp Ile Leu Val Gly Met Pro Val Val Arg Lys Pro Thr Glu 85 90 95 Thr Arg Arg Ser Val Asn His Thr Ile Ile Leu Lys Asn Ser Leu Ser 100 105 110 Ala Gly Ala Thr Phe Lys Thr Leu Leu Ser Glu Leu Arg Thr Ser Leu 115 120 125 Pro Glu Ala Ile Gln His Gln His Ile Pro Phe Leu Lys Met Thr Glu 130 135 140 Lys Leu Asp Leu Gln Tyr Ala Asp Gly Ile Pro Val Val His Thr Leu 145 150 155 160 Val Ser Leu Lys Glu Leu His Leu Asp Glu Ile Gly Gln Asn Val Val 165 170 175 Thr Asp Cys Ser Phe Glu Phe Ser Leu Thr Gly Gly Thr Ile Gln Leu 180 185 190 Ala Leu Ser Tyr Asn Glu His Leu Tyr Asp Ser Glu Phe Met Thr Arg 195 200 205 Val Val Gly His Leu Asn Arg Leu Leu Ala Val Gly Leu His Glu Leu 210 215 220 Glu Leu Glu Ile Val Arg Ala Asp Met Leu Ser Glu Asp Glu Lys Phe 225 230 235 240 Gln Leu Leu Gln Ser Phe Asn Asp Thr Glu Lys Asp Tyr Pro Arg Asp 245 250 255 Arg Thr Ile His Gln Leu Val Glu Glu Gln Ala Lys Arg Val Pro Glu 260 265 270 Ala Thr Ala Val Val Phe Glu Gly Arg Arg Leu Ser Tyr Ala Glu Leu 275 280 285 Asn Glu Arg Ala Asn Arg Leu Ala Arg Thr Leu Arg Ser Val Gly Val 290 295 300 Leu Pro Asn Gln Leu Val Gly Leu Met Ala Arg Arg Ser Leu Glu Thr 305 310 315 320 Val Val Gly Ile Leu Ala Val Leu Lys Ala Gly Gly Ala Tyr Val Pro 325 330 335 Ile Asp Pro Glu Tyr Pro Glu Glu Arg Ile Arg Tyr Ile Leu Glu Asn 340 345 350 Ser Asn Ala Gln Leu Leu Leu Thr Gln Arg Glu Leu Leu Gln Gln Val 355 360 365 Pro Phe Glu Gly Thr Val Leu Ala Leu Asp Asp Glu Gln Thr Tyr Ser 370 375 380 Asp Asp Gly Ser Asn Leu Glu Pro Ala Ser Gly Pro Asn Asp Leu Ala 385 390 395 400 Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly Lys Pro Lys Gly Val Met 405 410 415 Leu Glu His Arg Gly Leu Val Ser Leu Lys Leu Met Phe Ala Asp Arg 420 425 430 Leu Gly Ile Thr Glu His Asp Arg Ile Val Gln Phe Ala Ser Leu Ser 435 440 445 Phe Asp Ala Ser Cys Trp Glu Met Phe Lys Ala Leu Tyr Phe Gly Ala 450 455 460 Ala Leu Tyr Ile Pro Thr Ala Glu Thr Ile Leu Asp Thr Arg Leu Phe 465 470 475 480 Glu Ser Tyr Met Asn Glu His Ala Ile Thr Ala Ala Ile Leu Pro Pro 485 490 495 Thr Tyr Ser Ala Tyr Leu Asn Pro Asp Arg Leu Pro Ser Leu Thr Lys 500 505 510 Leu Val Thr Gly Gly Ser Ala Val Ser Ala Glu Phe Val Gln Gln Trp 515 520 525 Lys Pro Lys Val His Tyr Phe Asn Ala Tyr Gly Pro Thr Glu Ala Ser 530 535 540 Ile Val Thr Thr Leu Trp Asp Ala Asp Glu Glu Gln Ser Glu Arg Arg 545 550 555 560 Val Ile Pro Ile Gly Arg Pro Leu Ala Asn His Arg Ile Phe Ile Leu 565 570 575 Asp Ala His Leu Gln Leu Val Pro Pro Gly Val Asp Gly Glu Leu Cys 580 585 590 Val Ala Gly Val Gly Leu Ala Arg Gly Tyr Leu Asn His Pro Glu Leu 595 600 605 Thr Ala Glu Lys Phe Val Glu His Pro Phe Ala Pro Gly Glu Arg Leu 610 615 620 Tyr Arg Thr Gly Asp Leu Ala Arg Trp Leu Pro Asp Gly Asn Ile Glu 625 630 635 640 Tyr Leu Gly Arg Ile Asp His Gln Val Lys Ile Arg Gly Phe Arg Ile 645 650 655 Glu Ile Gly Glu Ile Glu Glu Gln Leu Leu Lys Ile Asp Ser Val Gln 660 665 670 Glu Thr Ile Val Ile Ala Arg Glu Gly Lys Ser Gly Gln Glu Leu Cys 675 680 685 Ala Tyr Leu Val Ala Gly His Pro Leu Thr Leu Gly Glu Leu Arg Ser 690 695 700 Ala Leu Ala Gln Lys Leu Pro Asn Tyr Met Ile Pro Ala His Phe Val 705 710 715 720 Gln Leu Pro Arg Met Pro Leu Thr Pro Asn Asp Lys Ile Asp Arg Lys 725 730 735 Ala Leu Pro Val Pro Glu Gly Asn Ala Leu Thr Gly Gly Ala Tyr Val 740 745 750 Ala Pro Arg Asn Glu Ala Glu Arg Thr Leu Ala Asp Val Trp Gln Ala 755 760 765 Val Leu Asn Ala Asp Arg Val Gly Val Thr Asp His Phe Phe Glu Leu 770 775 780 Gly Gly Asp Ser Ile Lys Ser Ile Gln Val Ser Ser Arg Leu His Gln 785 790 795 800 Ala Gly Tyr Lys Leu Glu Ile Arg Asp Leu Phe Lys Tyr Pro Thr Ile 805 810 815 Ser Gln Leu Ser Leu His Val Lys Pro Ile Gly Arg Thr Ile Asp Gln 820 825 830 Gly Glu Ile Thr Gly Glu Thr Ala Leu Thr Pro Ile Gln His Trp Phe 835 840 845 Phe Glu Ser Ser Phe Ala Asp Pro His His Phe Asn Gln Ser Val Ile 850 855 860 Leu Tyr Arg Lys Glu Arg Phe Asp Glu Glu Thr Val Arg Gln Val Leu 865 870 875 880 Gln Lys Leu Ala Glu His His Asp Ala Leu Arg Met Val Phe Arg Lys 885 890 895 Thr Glu Gln Gly Phe Ser Ala Arg Asn Arg Ala Ile Gln Glu Gly Gly 900 905 910 Leu Phe Thr Leu Asp Val Phe Asp Phe Lys Asp Ala Lys Asn Thr Ala 915 920 925 Gln Ala Val Glu Ala Lys Ala Thr Asp Ile Gln Ala Gly Ile Asp Leu 930 935 940 Glu Asn Gly Pro Leu Val Lys Ala Gly Leu Phe Arg Cys Ala Asp Gly 945 950 955 960 Asp His Leu Leu Leu Ala Val His His Ala Val Val Asp Gly Val Ser 965 970 975 Trp Arg Ile Leu Met Glu Asp Phe Ala Leu Gly Tyr Glu Gln Ala Gly 980 985 990 Lys Ser Glu Glu Ile Arg Phe Pro Ala Lys Thr Asp Ala Tyr Arg Thr 995 1000 1005 Trp Ser Glu Gln Leu Ala Ala Tyr Val Gln Ser Pro Glu Met Ala Lys 1010 1015 1020 Glu Arg Ala Tyr Trp Gln Ala Val Glu Gln Ile Ala Val Pro Ala Val 1025 1030 1035 1040 Pro Lys Asp Leu Glu Ala Asp Val Thr Thr Gln Gln Asp Ser Glu Ser 1045 1050 1055 Leu Phe Val Cys Leu Thr Pro Glu Glu Thr Glu Leu Leu Leu Lys Arg 1060 1065 1070 Val His Arg Ala Tyr Asn Thr Glu Met Asn Asp Ile Leu Val Thr Ala 1075 1080 1085 Leu Gly Ile Ala Ile Arg Lys Trp Thr Gly His Glu Arg Val Arg Ile 1090 1095 1100 Asn Leu Glu Gly His Gly Arg Glu Ser Ile Gly Thr Asp Ile Asp Ile 1105 1110 1115 1120 Thr Arg Thr Val Gly Trp Phe Thr Thr Lys Phe Pro Val Val Leu Glu 1125 1130 1135 Pro Glu Thr Asp Arg Asp Leu Ala Tyr Gln Ile Lys Gln Val Lys Glu 1140 1145 1150 Ser Leu Arg Arg Ile Pro Asn Lys Gly Leu Gly Tyr Gly Val Cys Arg 1155 1160 1165 Tyr Leu Ser Lys Ser Glu Asp Gly Phe Val Trp Gly Ala Glu Pro Glu 1170 1175 1180 Ile Asn Phe Asn Tyr Leu Gly Gln Phe Asp Asp Asp Val Asn Gln Asp 1185 1190 1195 1200 Glu Ile Gly Ile Ser Ser Tyr Ser Ser Gly Ser Pro Ala Ser Asp Arg 1205 1210 1215 Gln Ala Arg Ser Phe Val Leu Asp Ile Asn Gly Met Val Leu Asp Gly 1220 1225 1230 Ala Leu Ser Leu Asp Leu Ser Tyr Ser Arg Lys Gln Tyr Arg Lys Glu 1235 1240 1245 Thr Ile Glu Ala Phe Ala Gln Arg Leu Glu Gln Ser Leu Arg Glu Leu 1250 1255 1260 Ile Thr His Cys Ala Gly Lys Glu Asn Thr Glu Leu Thr Pro Ser Asp 1265 1270 1275 1280 Val Gln Phe Lys Gly Leu Thr Ile Ala Glu Leu Glu Gln Ile Ala Gln 1285 1290 1295 Arg Ser Gly His Leu Gly Glu Ile Glu Asn Ile Tyr Ser Leu Thr Pro 1300 1305 1310 Met Gln Lys Gly Met Trp Phe His Ser Ala Leu Asp Arg Gln Thr Ala 1315 1320 1325 Ala Tyr Phe Glu Gln Thr Arg Phe Thr Met Arg Gly Ala Leu Asp Val 1330 1335 1340 Gln Leu Phe Glu Arg Ser Trp Met Glu Leu Ala Lys Arg His Leu Val 1345 1350 1355 1360 Leu Arg Ala Asn Phe Val Lys Gly Pro Ala Gly Glu Pro Leu Gln Ile 1365 1370 1375 Ile Tyr Arg Asp Lys Pro Val Gly Phe Glu Tyr Glu Glu Leu Leu His 1380 1385 1390 Leu Gln Ala Asp Glu Lys Gln Thr Tyr Leu Asp Lys Lys Ala Glu Asp 1395 1400 1405 Asp Lys Leu Arg Gly Phe Asp Leu Glu His Asp Ala Leu Val Arg Val 1410 1415 1420 Thr Ile Leu Arg Thr Glu Glu Gln Ser Tyr His Val Leu Trp Ser Phe 1425 1430 1435 1440 Gln His Ile Leu Met Asp Gly Trp Cys Leu Pro Gln Leu Thr Gln Glu 1445 1450 1455 Leu Phe Glu Thr Tyr Ser Ala Leu Ala Ser Gly Lys Gln Pro Ala Gly 1460 1465 1470 Asp Lys Gly Ser Asp Tyr Gly Ala Tyr Ile Glu Trp Leu Glu Lys Gln 1475 1480 1485 Asp Asp Gln Ala Ala Ser Gly Tyr Trp Thr Ala Phe Leu Ala Gly Tyr 1490 1495 1500 Glu Gly Gln Thr Val Leu Pro Gly Gln Lys Glu Pro Ala Pro Asn Gly 1505 1510 1515 1520 Arg Phe Thr Ala Asp His Val Thr Ala Glu Leu Gly Lys Asp Leu Ser 1525 1530 1535 Glu Arg Met Asp Arg Val Ala Lys Gln Arg Leu Val Thr Val Asn Thr 1540 1545 1550 Leu Leu Gln Ala Ala Trp Gly Val Met Leu Gln Lys Tyr Asn Gly Thr 1555 1560 1565 Asn Asp Ala Val Phe Gly Ser Val Val Ala Gly Arg Pro Ala Glu Ile 1570 1575 1580 Pro Gly Ile Glu Ser Met Ile Gly Leu Phe Ile Asn Thr Val Pro Val 1585 1590 1595 1600 Arg Val Thr Ser Glu Ala Asp Thr Val Phe Ala Asp Leu Met Ala Lys 1605 1610 1615 Leu Gln Glu Arg Ala Leu Glu Ser Gly Arg Tyr Asp Tyr Tyr Pro Leu 1620 1625 1630 Tyr Glu Ile Gln Ala His Ser Val Gln Lys Gln Asn Leu Ile Asn His 1635 1640 1645 Ile Ile Ala Phe Glu Asn Tyr Pro Val Asp Glu Gln Met Glu Gln Ala 1650 1655 1660 Gly Asp Gln Gln His Gly Asp Leu Thr Ile Ala Asp Val Gln Met Glu 1665 1670 1675 1680 Glu Gln Thr Asn Tyr Asn Phe Asn Val Thr Val Val Pro Gly Asp Glu 1685 1690 1695 Ile Glu Ile Arg Phe Asp Phe Asn Ala Glu Val Phe Asp Lys Asp Ser 1700 1705 1710 Ile Glu Arg Leu Lys Gly His Leu Val His Leu Leu Glu Gln Val Thr 1715 1720 1725 Asp Asn Pro Glu Ile Thr Val Gly Glu Leu Glu Leu Val Thr Glu Ala 1730 1735 1740 Glu Lys Ala Asp Leu Leu Gly Arg Phe Asn Asp Thr Thr Thr Glu Phe 1745 1750 1755 1760 Pro Arg Gly Lys Thr Leu Ile Gln Leu Phe Glu Glu Gln Ala Glu Arg 1765 1770 1775 Ile Pro Asp Ala Ala Ala Ile Ser Leu Asn Glu Gln Glu Leu Thr Tyr 1780 1785 1790 Arg Glu Leu Asn Glu Arg Val Asn Arg Leu Ala Arg Thr Leu Arg Ser 1795 1800 1805 His Gly Ile Ser Lys Gly Arg Leu Val Ala Ile Met Ala Glu Arg Ser 1810 1815 1820 Ile Glu Met Val Val Gly Met Leu Ala Ala His Lys Ala Gly Ala Ala 1825 1830 1835 1840 Tyr Val Pro Ile Asp Pro Glu Tyr Pro Glu Glu Arg Ile Arg Phe Leu 1845 1850 1855 Ile Glu Asp Ser Gly Ala Gln Val Met Leu Thr Gln Ser Arg Leu Arg 1860 1865 1870 Glu Arg Leu Ala Gly Ser Asp Ser Val Ile Leu Leu Asp Asp Glu Ser 1875 1880 1885 Phe Tyr His Glu Asp Gly Thr Asn Leu Asn Pro Gly Thr Glu Ala Thr 1890 1895 1900 Asp Leu Ala Cys Val Ile Tyr Thr Ser Gly Thr Thr Gly Lys Pro Lys 1905 1910 1915 1920 Gly Asn Leu Val Ser His Arg Asn Ile Val Arg Ile Val Arg Asn Thr 1925 1930 1935 Asn Tyr Ile Asp Ile Thr Glu Arg Asp His Val Leu Gln Leu Ser Ser 1940 1945 1950 Tyr Ser Phe Asp Gly Ala Ile Phe Asp Val Phe Gly Ala Leu Thr Asn 1955 1960 1965 Gly Ala Arg Leu Val Leu Val Pro Arg Glu Thr Leu Leu Glu Ile Gly 1970 1975 1980 Arg Leu Ala Asp Leu Ile Gln His Glu Arg Ile Ser Val Met Leu Ile 1985 1990 1995 2000 Thr Thr Ala Tyr Phe Asn Val Leu Val Asp Val Asn Val Asp Cys Leu 2005 2010 2015 Arg Asp Val Arg Ala Ile Leu Phe Gly Gly Glu Arg Val Ser Val Gly 2020 2025 2030 His Val Arg Arg Ala Leu Ala His Ile Gly Pro Gly Arg Leu Asn His 2035 2040 2045 Leu Tyr Gly Pro Ser Glu Ser Thr Val Tyr Thr Thr Tyr Leu Pro Val 2050 2055 2060 Asp Phe Val Asp Glu Ser Ala Val Thr Val Pro Ile Gly Arg Pro Ile 2065 2070 2075 2080 Ser Asn Thr Thr Val Tyr Ile Val Asp Ser Arg Asn Lys Leu Leu Pro 2085 2090 2095 Ile Gly Val Ala Gly Glu Leu Cys Val Gly Gly Glu Gly Leu Val Arg 2100 2105 2110 Gly Tyr Asn Asn Arg Pro Glu Leu Thr Ala Glu Lys Phe Val Asp Asn 2115 2120 2125 Pro Phe Val Pro Gly Glu Arg Met Tyr Arg Thr Gly Asp Leu Ala Lys 2130 2135 2140 Trp Leu Pro Asp Gly Thr Ile Glu Tyr Val Gly Arg Thr Asp Asp Gln 2145 2150 2155 2160 Val Lys Ile Arg Gly Phe Arg Ile Glu Leu Gly Glu Ile Glu Ala Gln 2165 2170 2175 Leu Gln Lys Val Glu Gly Ile Arg Lys Thr Thr Val Phe Ala Arg Glu 2180 2185 2190 Asn Ala Ser Gly Glu Lys Gln Leu Cys Ala Tyr Tyr Glu Ala Asp Arg 2195 2200 2205 Glu Leu Ser Ala Ala Glu Leu Lys Asn Val Ile Ser Gln Glu Leu Pro 2210 2215 2220 Ala Tyr Met Ile Pro Ala Tyr Leu Ile Gln Leu Glu Arg Leu Pro Leu 2225 2230 2235 2240 Thr Ala Asn Gly Lys Val Asp Arg Arg Ser Leu Pro Thr Pro Glu Glu 2245 2250 2255 Ser Leu Gln Pro Gly Glu Gly Arg Thr Pro Pro Arg Thr Pro Leu Glu 2260 2265 2270 Ala Ser Leu Ala Gly Ile Trp Lys Ser Val Leu Ala Leu Glu His Ile 2275 2280 2285 Gly Val His Asp Asn Phe Phe Asp Leu Gly Gly His Ser Leu Arg Ala 2290 2295 2300 Thr Thr Leu Val Ser Lys Val His Gln Glu Leu Asn Val Glu Leu Pro 2305 2310 2315 2320 Leu Arg Asp Val Phe Arg Tyr Ser Thr Ile Glu Glu Met Ala Leu Ala 2325 2330 2335 Ile Thr Arg Ile Gly Glu Gln Ser Phe Ser Ser Ile Pro Leu Ala Gly 2340 2345 2350 Val Arg Glu Tyr Tyr Pro Leu Ser Ser Ala Gln Lys Arg Leu Phe Ile 2355 2360 2365 Leu Asn Gln Leu Glu Gly Ser Asp Gln Ser Tyr Asn Met Pro Gly Val 2370 2375 2380 Leu Leu Leu Glu Gly Ser Ile Asp Arg Ser Leu Leu Glu Lys Ala Phe 2385 2390 2395 2400 Arg Gly Leu Ile Val Arg His Glu Thr Leu Arg Thr Gly Phe Glu Ile 2405 2410 2415 Val Gln Gly Glu Ala Val Gln Arg Ile Tyr Glu Ser Val Asp Phe Ala 2420 2425 2430 Val Glu Tyr Arg His Ala Ser Glu Glu Glu Ala Pro Glu Val Val Gln 2435 2440 2445 Ala Phe Ile Arg Pro Phe Asp Leu Ala Lys Pro Pro Leu Leu Arg Val 2450 2455 2460 Glu Leu Val Glu Leu Ala Thr Glu Arg Tyr Leu Leu Met Phe Asp Met 2465 2470 2475 2480 His His Ile Val Ser Asp Gly Val Ser Met Asp Val Leu Val Glu Glu 2485 2490 2495 Leu Val Arg Leu Tyr Gly Gly Glu Ser Leu Glu Pro Leu Arg Ile Gln 2500 2505 2510 Tyr Lys Asp Tyr Ala Val Trp Gln Gln Ser Asp Glu Gln Lys Ala Gln 2515 2520 2525 Leu Lys Arg Glu Glu Ala Tyr Trp Leu Asp Arg Tyr Arg Gly Glu Leu 2530 2535 2540 Pro Val Leu Glu Met Pro Thr Asp Tyr Pro Arg Pro Ala Val Gln Ser 2545 2550 2555 2560 Phe Glu Gly Gln Thr Leu Thr Ser Phe Val Asp Glu Ala Thr Asn Glu 2565 2570 2575 Gly Leu Lys Gln Leu Ala Ala Gln Arg Gly Thr Thr Leu Tyr Met Val 2580 2585 2590 Leu Leu Ala Ala Tyr Thr Val Leu Leu His Lys Tyr Thr Gly Gln Asp 2595 2600 2605 Asp Leu Ile Val Gly Thr Ser Ile Ala Gly Arg Thr His Gly Asp Thr 2610 2615 2620 Gln Pro Leu Ile Gly Met Phe Val Asn Thr Leu Ala Leu Arg Asn Tyr 2625 2630 2635 2640 Pro Ala Ser Glu Lys Thr Phe Leu Ser Tyr Leu Glu Glu Val Lys Glu 2645 2650 2655 Thr Thr Leu Gly Ala Tyr Glu His Gln Asn Tyr Pro Phe Glu Glu Leu 2660 2665 2670 Val Asp Lys Val Gln Val Ser Arg Asp Leu Ser Arg Asn Pro Leu Phe 2675 2680 2685 Asp Thr Met Phe Ser Leu Gln Asn Leu Glu Asp Lys Glu Phe Glu Leu 2690 2695 2700 Glu Gly Leu Lys Leu Ser Pro Tyr Pro Ser Glu Tyr Gly Thr Ala Lys 2705 2710 2715 2720 Phe Asp Leu Ser Val Asp Val Thr Glu Glu Asn Gly Gly Leu Glu Cys 2725 2730 2735 Ser Phe Glu Phe Ala Thr Ala Leu Tyr Lys Glu Ser Thr Ile Arg Arg 2740 2745 2750 Leu Ser Thr His Phe Gly His Leu Leu Ala Glu Ile Val Ser Cys Pro 2755 2760 2765 Asp Ala Lys Ile Ala Glu Leu Asn Leu Leu Thr Ala Glu Glu Lys Glu 2770 2775 2780 Gln Ile Leu Gly Ala Phe Asn Pro Ala Gln Pro Glu Val Ala Pro Ala 2785 2790 2795 2800 Ala Ala Phe His Arg Leu Phe Glu Glu Gln Ala Glu Arg Thr Pro Glu 2805 2810 2815 Ala Ala Ala Val Val Tyr Glu Asn Asp Arg Leu Thr Tyr Ala Glu Leu 2820 2825 2830 Asn Glu Arg Ala Asn Arg Leu Ala Ala Thr Leu Arg Ala Ser Gly Ile 2835 2840 2845 Gly Arg Glu Thr Ile Val Gly Ile Leu Ala Glu Arg Ser Val Asp Leu 2850 2855 2860 Leu Val Ala Val Leu Ala Val Trp Lys Ala Gly Gly Ala Tyr Val Pro 2865 2870 2875 2880 Leu Asp Pro Asp Tyr Pro Ala Glu Arg Val Arg Phe Met Leu Glu Asp 2885 2890 2895 Ser Gly Ala Lys Val Leu Leu Thr Gln Thr Ala Leu Arg Glu Arg Ala 2900 2905 2910 Glu Ala Trp Leu Gly Glu Glu Glu Leu Ala Leu Ala Ala Val Leu Tyr 2915 2920 2925 Leu Asp Asp Glu Ala Ser Tyr Ser Glu Glu Arg Ala Asn Ala Pro Ile 2930 2935 2940 Val Phe Gly Ile Ser Ser Lys Leu Thr Asp Ala Val Asp Asp Gly Asp 2945 2950 2955 2960 Glu Ser Pro Gln Asn Val Gly Thr Asp Ser Phe His Glu Ala Arg Pro 2965 2970 2975 Glu Asp Leu Ala Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly Lys Pro 2980 2985 2990 Lys Gly Val Met Ile Glu His Arg Ser Leu Val Asn Thr Ala Ala Gly 2995 3000 3005 Tyr Arg Gln Glu Tyr Arg Leu Asp Gln Phe Pro Val Arg Leu Leu Gln 3010 3015 3020 Leu Ala Ser Phe Ser Phe Asp Val Phe Val Gly Asp Ile Ala Arg Thr 3025 3030 3035 3040 Leu Tyr Asn Gly Gly Thr Met Val Ile Val Pro Lys Asp Asp Arg Ile 3045 3050 3055 Asp Pro Ser Arg Leu His His Trp Ile Glu Gln Glu Arg Val Thr Ile 3060 3065 3070 Phe Glu Ser Thr Pro Ala Leu Ile Val Pro Phe Leu Glu Tyr Val His 3075 3080 3085 Glu His Arg Leu Asp Met Ser Trp Met Glu Leu Leu Ile Thr Ser Ser 3090 3095 3100 Asp Ser Cys Ser Val Ala Asp Tyr Arg Thr Leu Gln Glu Arg Phe Gly 3105 3110 3115 3120 Ser Leu Phe Arg Ile Val Asn Ala Tyr Gly Val Thr Glu Ala Ala Ile 3125 3130 3135 Asp Ser Ser Phe Tyr Asp Glu Pro Leu Ala Lys Leu Pro Gln Thr Gly 3140 3145 3150 His Val Pro Ile Gly Lys Ala Trp Leu Asn Ala Lys Phe Tyr Ile Val 3155 3160 3165 Asp Ala His Leu Asn Pro Val Pro Val Gly Val Leu Gly Glu Leu Val 3170 3175 3180 Ile Gly Gly Val Gly Val Ala Arg Gly Tyr Leu Asn Arg Pro Glu Leu 3185 3190 3195 3200 Thr Glu Glu Lys Phe Val Asp Ser Pro Phe Ala Ala Gly Glu Arg Leu 3205 3210 3215 Tyr Arg Thr Gly Asp Leu Ala Arg Trp Met Glu Asp Gly Asn Val Asp 3220 3225 3230 Phe Ile Gly Arg Ile Asp Asn Gln Ala Lys Ile Arg Gly Tyr Arg Ile 3235 3240 3245 Glu Thr Gly Glu Ile Glu Ser Gln Leu Leu Arg Val Lys Gly Val Arg 3250 3255 3260 Glu Ala Val Val Leu Val Leu Ser Asp Ala Asn Gly Gln Lys Ala Leu 3265 3270 3275 3280 Cys Ala Tyr Tyr Thr Pro Asp Thr Gly Ala Glu Leu Ala Val Asn Asp 3285 3290 3295 Leu Arg Ser Thr Leu Ala Gln Glu Leu Pro Gly Tyr Met Ile Pro Ser 3300 3305 3310 Tyr Phe Val Glu Leu Glu His Leu Pro Leu Thr Pro Asn Gly Lys Ile 3315 3320 3325 Asp Arg Lys Ala Leu Pro Ala Pro Glu Gly Glu Ala Gly Asn Gly Thr 3330 3335 3340 Glu Tyr Val Ala Pro Arg Asn Glu Leu Glu Thr Lys Leu Ala Ala Ile 3345 3350 3355 3360 Trp Gln Glu Val Leu Gly Leu Ala Lys Glu Ile Gly Val His Asp Asn 3365 3370 3375 Phe Phe Asp Ile Gly Gly His Ser Leu Arg Ala Thr Thr Leu Val Ser 3380 3385 3390 Lys Ile His Lys Glu Leu Asn Val Asp Leu Pro Leu Arg Asp Val Phe 3395 3400 3405 Arg His Ser Thr Ile Glu Ser Met Ala Ala Ala Ile Ser Arg Leu Asp 3410 3415 3420 Glu Gln Thr Phe Val Ser Ile Pro Val Ala Asp Asp Arg Glu Val Tyr 3425 3430 3435 3440 Pro Gln Ser Phe Ala Gln Lys Arg Leu Phe Ile Leu Asn Gln Leu Glu 3445 3450 3455 Gly Ala Glu Leu Ser Tyr Asn Met Pro Glu Ala Met Leu Leu Glu Gly 3460 3465 3470 Ala Leu Asp Arg Ala Arg Phe Glu Glu Ala Phe Arg Lys Leu Val Thr 3475 3480 3485 Arg His Glu Thr Leu Arg Thr Gly Phe Glu Met Val Asp Gly Glu Ala 3490 3495 3500 Ser Gln Arg Ile Tyr Gln Asp Val Asn Val Ala Val Glu Phe Tyr Arg 3505 3510 3515 3520 Ala Asp Lys Gln Glu Ala Glu Glu Thr Val Arg Arg Phe Val Arg Pro 3525 3530 3535 Phe Asp Leu Ala Lys Pro Pro Leu Leu Arg Val Gly Leu Val Glu Leu 3540 3545 3550 Ala Pro Glu Arg His Ile Leu Met Tyr Asp Met His His Ile Ile Ser 3555 3560 3565 Asp Gly Val Ser Met Glu Ile Phe Val Glu Glu Phe Val Arg Leu Tyr 3570 3575 3580 Gly Gly Glu Gln Leu Asp Pro Leu Arg Ile Gln Tyr Lys Asp Tyr Thr 3585 3590 3595 3600 Val Trp Gln His Ser Gln Glu Gln Lys Glu Arg Leu Gln Arg Gln Glu 3605 3610 3615 Ala Tyr Trp Leu Asp Met Phe Gln Gly Glu Leu Pro Val Leu Glu Met 3620 3625 3630 Pro Thr Asp Tyr Pro Arg Pro Ala Val Gln Ser Tyr Glu Gly Gln Thr 3635 3640 3645 Leu Glu Phe Phe Phe Asp Ala Ser Lys Thr Asp Gly Leu Lys Gln Leu 3650 3655 3660 Ala Ser Glu Thr Gly Thr Thr Leu Phe Met Val Leu Leu Ala Ala Tyr 3665 3670 3675 3680 Asn Val Leu Leu His Lys Tyr Ser Gly Gln Glu Asp Val Ile Val Gly 3685 3690 3695 Thr Pro Ile Ala Gly Arg Asn His Gly Asp Val Gln Pro Leu Ile Gly 3700 3705 3710 Ile Phe Leu Asn Thr Leu Ala Ile Arg Ser Tyr Pro Ala Ser Glu Lys 3715 3720 3725 Thr Phe Leu Ser Tyr Leu Asn Glu Val Lys Glu Thr Thr Leu His Ala 3730 3735 3740 Phe Glu His Gln Asn Tyr Pro Phe Glu Glu Leu Val Asp Lys Val Gln 3745 3750 3755 3760 Val Thr Arg Asp Leu Ser Arg Asn Pro Leu Phe Asp Thr Leu Phe Thr 3765 3770 3775 Met Gln Asn Thr Glu Asn Glu Glu Phe Glu Leu Glu Gly Leu Arg Leu 3780 3785 3790 Ile Pro Tyr Pro Ser Ala Leu Asp Thr Ala Lys Phe Asp Ile Ser Leu 3795 3800 3805 Asp Val Gly Glu Glu Asn Gly Gly Leu Asp Tyr Ser Phe Glu Tyr Ala 3810 3815 3820 Thr Ala Leu Tyr Lys Arg Glu Thr Ile Glu Arg Leu Ala Lys His Tyr 3825 3830 3835 3840 Glu Gln Leu Leu Val Thr Ile Val Ser Arg Pro Asp Ala Lys Ile Ala 3845 3850 3855 Glu Leu Asn Leu Leu Thr Ala Glu Glu Lys Glu Gln Ile Leu Gly Ala 3860 3865 3870 Phe Asn Pro Ala Gln Pro Glu Ala Thr Ser Ala Ala Ala Phe His Arg 3875 3880 3885 Leu Phe Glu Glu Gln Ala Glu Arg Thr Pro Glu Thr Ala Ala Val Val 3890 3895 3900 Tyr Glu Asn Asp Arg Leu Thr Tyr Ala Glu Leu Asn Glu Arg Ala Asn 3905 3910 3915 3920 Arg Leu Ala Ala Thr Leu Arg Thr Ser Gly Ile Gly Arg Glu Thr Ile 3925 3930 3935 Val Gly Ile Leu Ala Glu Arg Ser Val Asp Leu Leu Val Ala Val Leu 3940 3945 3950 Ala Val Trp Lys Ala Gly Gly Ala Tyr Val Pro Leu Asp Pro Asp Tyr 3955 3960 3965 Pro Ala Glu Arg Val Arg Phe Met Leu Glu Asp Ser Gly Ala Lys Val 3970 3975 3980 Leu Leu Thr Gln Thr Ala Leu Arg Glu Arg Ala Glu Ala Trp Leu Gly 3985 3990 3995 4000 Glu Glu Glu Leu Ala Leu Ala Ala Val Leu Tyr Leu Asp Asp Glu Thr 4005 4010 4015 Ser Tyr Ser Glu Glu Arg Ala Asn Ala Pro Ile Val Ser Gly Met Val 4020 4025 4030 Ser Gly Lys Leu Thr Asp Ala Val Asp Asp Gly Asp Glu Ser His Gln 4035 4040 4045 Asn Val Gly Thr Asp Ser Phe His Glu Ala Arg Pro Glu Asp Leu Ala 4050 4055 4060 Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly Lys Pro Lys Gly Val Met 4065 4070 4075 4080 Ile Glu His Arg Ser Leu Val Asn Thr Ala Ala Gly Tyr Arg Arg Lys 4085 4090 4095 Tyr Arg Leu Asp Gln Phe Pro Val Arg Leu Leu Gln Leu Ala Ser Phe 4100 4105 4110 Ser Phe Asp Val Phe Val Gly Asp Ile Ala Arg Thr Leu Tyr Asn Gly 4115 4120 4125 Gly Thr Met Val Ile Val Pro Lys Asp Asp Arg Ile Asp Pro Ser Arg 4130 4135 4140 Leu His His Trp Ile Glu Gln Glu Arg Val Thr Ile Phe Glu Ser Thr 4145 4150 4155 4160 Pro Ala Leu Ile Val Pro Phe Leu Glu Tyr Val His Glu His Arg Leu 4165 4170 4175 Asp Met Ser Trp Met Glu Leu Leu Ile Thr Asn Ser Asp Ser Cys Ser 4180 4185 4190 Val Ala Asp Tyr Arg Thr Leu Gln Glu Arg Phe Gly Ser Leu Phe Arg 4195 4200 4205 Ile Val Asn Ala Tyr Gly Val Thr Glu Ala Ala Ile Asp Ser Ser Phe 4210 4215 4220 Tyr Asp Glu Glu Leu Ala Lys Leu Pro Gln Thr Gly Asn Val Pro Ile 4225 4230 4235 4240 Gly Lys Ala Trp Leu Asn Ala Lys Phe Tyr Ile Val Asp Ala His Leu 4245 4250 4255 Asn Pro Val Pro Val Gly Val Leu Gly Glu Leu Val Ile Gly Gly Ile 4260 4265 4270 Gly Val Ala Arg Gly Tyr Leu Asn Arg Pro Glu Leu Thr Glu Glu Lys 4275 4280 4285 Phe Val Asp Ser Pro Phe Ala Ala Gly Glu Arg Leu Tyr Arg Thr Gly 4290 4295 4300 Asp Leu Ala Arg Trp Met Glu Asp Gly Asn Val Asp Phe Ile Gly Arg 4305 4310 4315 4320 Ile Asp Asn Gln Ala Lys Ile Arg Gly Tyr Arg Ile Glu Thr Gly Glu 4325 4330 4335 Ile Glu Ser Gln Leu Leu Arg Val Glu Gly Val Arg Glu Ala Val Val 4340 4345 4350 Leu Val Arg Ser Asp Ala Asn Gly Gln Lys Ala Leu Cys Ala Tyr Tyr 4355 4360 4365 Thr Leu Asp Thr Gly Ala Glu Leu Ala Val Asn Asp Leu Arg Ser Ala 4370 4375 4380 Leu Ala Gln Glu Leu Pro Gly Tyr Met Ile Pro Ser Tyr Phe Val Glu 4385 4390 4395 4400 Leu Gly Arg Leu Pro Leu Thr Pro Asn Gly Lys Ile Asp Arg Lys Ala 4405 4410 4415 Leu Pro Ala Pro Glu Gly Glu Ala Gly Ser Gly Thr Glu Tyr Val Ala 4420 4425 4430 Pro Arg Asn Glu Leu Glu Thr Lys Leu Ala Ala Ile Trp Gln Glu Val 4435 4440 4445 Leu Gly Leu Ala Lys Glu Ile Gly Val His Asp Asn Phe Phe Asp Ile 4450 4455 4460 Gly Gly His Ser Leu Arg Ala Thr Thr Leu Ala Gly Lys Val Phe Lys 4465 4470 4475 4480 Glu Leu Asn Val Asn Leu Pro Leu Arg Asp Val Phe Arg His Ser Thr 4485 4490 4495 Ile Ala Ala Met Ala Glu Ala Ile Ala Arg Met Glu Arg Arg Glu His 4500 4505 4510 Glu Ala Ile Pro Gln Ala Glu Glu Arg Glu Tyr Tyr Pro Leu Ser Ser 4515 4520 4525 Ala Gln Lys Arg Leu Phe Ile Gln His Thr Leu Asp Gly Ala Asp Gln 4530 4535 4540 Leu Tyr Asn Met Pro Glu Leu Val Gln Val Glu Gly Glu Phe Asp Leu 4545 4550 4555 4560 Asp Arg Leu Glu Ala Ala Leu Arg Lys Leu Ile Thr Arg His Glu Ser 4565 4570 4575 Leu Arg Thr Gly Phe Glu Ile Val Lys Gly Glu Ala Val Gln Arg Val 4580 4585 4590 Tyr Pro Gln Val Asp Phe Ala Ile Glu His His Gln Ala Asp Lys Glu 4595 4600 4605 Asp Ala Ala Gln Ile Glu Gln Ile Val Arg Ser Phe Ile Arg Pro Phe 4610 4615 4620 Asp Leu Gly Lys Pro Pro Leu Leu Arg Ala Gly Val Ile Glu Leu Glu 4625 4630 4635 4640 Pro Asn Leu His Ile Leu Leu Phe Asp Met His His Met Val Ser Asp 4645 4650 4655 Gly Val Ser Met Ala Ile Val Ile Asp Glu Phe Ser Ser Phe Tyr Ala 4660 4665 4670 Gly Glu Glu Leu Pro Pro Leu Arg Ile Gln Tyr Lys Asp Tyr Ala Val 4675 4680 4685 Trp Gln Gln Ser Lys Ala His Arg Glu Arg Ile Gly Arg Gln Glu Ala 4690 4695 4700 Tyr Trp Leu Gln Thr Phe Glu Gly Glu Leu Pro Thr Ala Asp Leu Pro 4705 4710 4715 4720 Ile Asp Tyr Glu Arg Ser Ala Ala Arg Ser Tyr Glu Gly Ala His Leu 4725 4730 4735 Glu Phe Asn Val Glu Ala Ser Leu Ser Ala Arg Leu Arg Glu Leu Ala 4740 4745 4750 Ala Glu Arg Glu Ser Thr Leu Phe Met Val Leu Leu Ala Ala Tyr Thr 4755 4760 4765 Val Leu Leu Ser Lys Tyr Ser Gly Gln Glu Asp Leu Ile Val Gly Thr 4770 4775 4780 Pro Val Ala Gly Arg Thr Asn Ala Asp Leu Glu Ser Val Ile Gly Met 4785 4790 4795 4800 Phe Val Asn Thr Leu Ala Ile Arg Asn Arg Pro Ser Gly Asp Lys Thr 4805 4810 4815 Phe Leu Ser Tyr Leu Glu Glu Val Lys Glu Thr Ala Leu Gly Ala Phe 4820 4825 4830 Glu Asn Gln Asp Tyr Pro Phe Glu Glu Leu Val Glu Arg Leu Asn Val 4835 4840 4845 Lys Arg Glu Pro Gly Arg Phe Pro Leu Phe Asp Ala Val Phe Asp Leu 4850 4855 4860 Gln Asn Ile Glu Glu Arg Asp Ala Glu Leu Glu Gly Val Ser Leu Lys 4865 4870 4875 4880 Thr Tyr Glu Leu Asp His Leu Glu Glu Ala Lys Phe Asp Leu Thr Leu 4885 4890 4895 Phe Met Tyr Glu Asn Asn Gly Ala Leu Ser Gly Gly Phe Phe Tyr Ala 4900 4905 4910 Thr Lys Leu Phe Lys Glu Ala Met Ile Arg Thr Leu Thr Glu Asp Tyr 4915 4920 4925 Leu Arg Val Leu Ser Gln Ile Ala Glu Asn Pro Gln Leu Glu Leu Ser 4930 4935 4940 Arg Ile Glu Cys His Lys Pro Ala Ala Gly Ala Lys Ser Ala Val Asp 4945 4950 4955 4960 Thr Ile Glu Phe Ala Phe 4965 <210> 5 <211> 1102 <212> PRT <213> Paenibacillus polymyxa ATCC21830 <400> 5 Met Lys Ser Leu Phe Glu Lys Glu Glu Arg Tyr Trp Ser Gly Lys Phe 1 5 10 15 Asp Ala Asp Asp Ser Leu Ser Phe Leu Pro Tyr Ser Gln Ser Ser Lys 20 25 30 Leu Ser Ala Asp Gly Glu Ala Ala Ala Glu Pro Gly Leu Leu His Arg 35 40 45 Thr Leu Pro Ser Glu Ile Ser Glu Arg Ile Ile Cys Leu Ala Asn Gly 50 55 60 Ser Asp Leu Ala Leu Tyr Met Ile Val Leu Ala Gly Val Lys Ser Leu 65 70 75 80 Leu Phe Lys Tyr Thr Gly Arg Asp Gln Val Leu Val Gly Met Pro Ser 85 90 95 Tyr Ser Ala Asp Pro Asp Gly Thr Pro Pro Pro His Asp Ile Leu Val 100 105 110 Ile Lys Thr Ser Val Ser Arg Glu Thr Thr Leu Arg Thr Leu Leu Gly 115 120 125 Gly Ile Lys Ala Ser Ile Gly Glu Ala Leu Glu His Gln His Leu Pro 130 135 140 Phe Arg Lys Met Val Glu Pro Leu His Leu Asp Tyr Thr Gly Asp Gly 145 150 155 160 Leu Pro Val Val Asn Thr Val Ala Ser Phe Ala Pro Ile His Pro Glu 165 170 175 Pro Leu Gly Asn Arg Val Ala Ala Asp Thr Val Phe Arg Phe Asp Arg 180 185 190 Gln Asn His Ser Ile Glu Leu Glu Ile Ser Phe Asp Gly Gln Arg Tyr 195 200 205 Glu Arg Ala Phe Val Glu Gln Ala Ala Asp His Leu Val Gln Leu Leu 210 215 220 Ser Val Leu Leu Phe Gln Pro Asp Leu Glu Leu Gly Gln Ala Asp Val 225 230 235 240 Leu Ser Pro Asp Glu Arg Glu Thr Leu Leu Lys Arg Phe Asn Asp Thr 245 250 255 Glu Thr Gly Phe Glu Arg Gly Lys Thr Ile Tyr Gly Leu Phe Glu Glu 260 265 270 Gln Ala Glu Leu Tyr Pro Asp Asn Val Ala Ala Val Met Asn Glu Arg 275 280 285 Gln Leu Thr Tyr Arg Glu Leu Asn Glu Arg Ser Asn Arg Leu Ala Arg 290 295 300 Lys Leu Arg Glu Thr Gly Val Glu Ala Asp Gln Leu Val Ala Ile Leu 305 310 315 320 Ala Glu Arg Ser Leu Asp Met Val Val Gly Ile Leu Ala Ile Leu Lys 325 330 335 Ala Gly Gly Ala Tyr Val Pro Val Asp Pro Asp Tyr Pro Glu Glu Arg 340 345 350 Ile Arg Phe Met Ile Glu Asp Ser Gly Ala Pro Leu Leu Leu Ile Gln 355 360 365 Lys His Leu His Glu Lys Thr Asp Phe Ala Gly Thr Arg Leu Glu Leu 370 375 380 Asp Asp Phe Val Trp Gly Asp Arg Gly Ala Asp Ser Glu Gly Ala Leu 385 390 395 400 Asp Ala Ser Asn Leu Glu Pro Ile Ser Gly Pro Val Asn Leu Ala Tyr 405 410 415 Val Ile Tyr Thr Ser Gly Thr Thr Gly Arg Pro Lys Gly Thr Leu Ile 420 425 430 Glu His Lys Asn Val Val Arg Leu Leu Phe Asn Asp Lys Asn Leu Phe 435 440 445 Asp Phe Gly Pro Ser Asp Thr Trp Thr Leu Phe His Ser Phe Cys Phe 450 455 460 Asp Phe Ser Val Trp Glu Met Tyr Gly Ala Leu Leu Asn Gly Gly Lys 465 470 475 480 Leu Val Ile Val Pro Pro Leu Thr Ala Lys Asn Pro Ala Asp Phe Leu 485 490 495 Ala Leu Leu Gly Arg Glu Gln Val Thr Ile Leu Asn Gln Thr Pro Thr 500 505 510 Tyr Phe Tyr Gln Leu Leu Arg Lys Val Leu Ala Asp His Pro Tyr Asp 515 520 525 Leu Arg Ile Arg Asn Val Ile Phe Gly Gly Glu Ala Leu Ser Pro Leu 530 535 540 Leu Leu Lys Gly Phe Lys Thr Lys Tyr Pro Glu Thr Lys Leu Ile Asn 545 550 555 560 Met Tyr Gly Ile Thr Glu Thr Thr Val His Val Thr Tyr Lys Glu Ile 565 570 575 Thr Trp Val Glu Met Glu Ala Ala Lys Ser Asn Ile Gly Lys Pro Ile 580 585 590 Pro Thr Leu Arg Val Tyr Val Leu Asp Glu Asn Arg Arg Pro Val Pro 595 600 605 Ile Gly Val Ala Gly Glu Met Tyr Val Ala Gly Ala Gly Leu Ala Arg 610 615 620 Gly Tyr Leu Asn Arg Pro Asp Leu Thr Ala Glu Lys Phe Val Asp Ser 625 630 635 640 Pro Phe Ala Glu Gly Glu Lys Leu Tyr Arg Ser Gly Asp Leu Ala Ala 645 650 655 Trp Leu Pro Asp Gly Asn Ile Glu Tyr Leu Gly Arg Ile Asp His Gln 660 665 670 Val Lys Ile Arg Gly Tyr Arg Ile Glu Leu Asp Glu Ile Glu Thr Gln 675 680 685 Leu Leu Lys Ile Ala Ala Val Gln Glu Ala Lys Val Leu Asp Arg Asp 690 695 700 Asp Ala Asn Gly Gln Lys Gln Leu Val Ala Tyr Tyr Val Ala Glu Thr 705 710 715 720 Arg Leu Ala Val His Glu Leu Lys Glu Glu Leu Ala Lys Gln Leu Pro 725 730 735 Gly Tyr Met Ile Pro Ser His Leu Val Gln Leu Pro Arg Met Pro Leu 740 745 750 Thr Pro Asn Gly Lys Ile Asp Arg Lys Ala Leu Pro Ala Pro Glu Glu 755 760 765 Ala Ala Ala Arg Gly Ala Glu Tyr Val Ala Pro Arg Thr Leu Leu Glu 770 775 780 Met Lys Ile Ala Arg Val Trp Gln Asp Thr Leu Gly Val Pro Gln Val 785 790 795 800 Gly Val Lys Asp Asn Phe Phe Glu Leu Gly Gly Asn Ser Leu Ser Leu 805 810 815 Met Arg Leu Val Gln Ala Val Tyr Asp Glu Thr Gly Ile Glu Ile Pro 820 825 830 Leu Asn Arg Gln Phe His Asn Val Thr Val Glu Ala Met Ala Phe Gly 835 840 845 Glu Gly Asp Leu Gly Leu Asp Lys Gly Gly Asp Ser Phe Ile Lys Leu 850 855 860 Asn Lys Val Gly Asn Leu Asn Val Phe Cys Phe Pro Pro Gly Ser Gly 865 870 875 880 Phe Gly Ile Gly Tyr Arg Glu Leu Ala Ser Arg Leu Asp Gly Arg Ser 885 890 895 Val Leu Tyr Ser Ile Asp Phe Ile Asp Asp Ala Val Asp Tyr Glu Ala 900 905 910 Met Leu Asn Arg Tyr Val Asp Glu Ile Val Arg Ile Gln Pro Glu Gly 915 920 925 Pro Tyr Val Leu Leu Gly Tyr Cys Phe Gly Gly Asn Leu Thr Phe Glu 930 935 940 Val Ala Lys Thr Met Glu Lys Arg Gly Tyr Ser Val Thr Asp Val Leu 945 950 955 960 Met Val Asp Ser Trp Ile Lys His Thr Leu Thr Pro Ser Glu Thr Ser 965 970 975 Glu Lys Glu Leu Glu Glu Thr Leu Ala Asp Phe Asp Glu Glu Glu Lys 980 985 990 Glu Leu Met Ser Asn Pro Leu Val Arg Glu Arg Val His Arg Lys Val 995 1000 1005 Lys Ala Thr Leu Ala Tyr Glu Ala Gln Leu Ile Asn Ser Gly Thr Ile 1010 1015 1020 Pro Ala Arg Ile Tyr Glu Leu Ile Ala Lys Asp Ser Glu Ala Phe Arg 1025 1030 1035 1040 Leu Glu His Gln Leu Pro Ser Trp Arg Gly Ala Thr Thr Gln Ala Tyr 1045 1050 1055 Ala Asp Tyr Arg Leu Glu Gly Ala His Glu Glu Leu Leu Glu Leu Ala 1060 1065 1070 Arg Val Asp Glu Thr Ala Val Val Ile Arg Asp Ile Leu Glu Gln Val 1075 1080 1085 Lys Arg Gln Ile Glu Ala Glu Ala Gly Val Leu His Gly Ser 1090 1095 1100 <210> 6 <211> 5789 <212> PRT <213> Paenibacillus polymyxa ATCC21830 <400> 6 Met Arg Glu Asp Thr Asn Glu Gln Tyr Gly Leu Thr Gln Ala Gln Arg 1 5 10 15 Arg Ile Trp Phe Met Glu Ile Met Asn Pro Gly Thr Ser Ile Thr Met 20 25 30 Leu Ser Ala Thr Tyr Gln Ile Thr Gly Glu Ile Asn Thr Gln Leu Leu 35 40 45 Lys Gln Ala Ala Ala Glu Ile Val Lys Thr Tyr Asp Val Phe Arg Ile 50 55 60 Arg Ile Ser Gly Asp Leu Gln Asn Pro Thr Gln Trp Phe Glu Glu Pro 65 70 75 80 Glu Asn Val Gln Ala Arg Ile Ser Arg Leu Glu Ile Gly Thr Thr Glu 85 90 95 Gln Phe Tyr Ala Trp Val Lys Glu Val Ser Glu Lys Pro Ala Ser Val 100 105 110 Phe Asp Glu His Leu Tyr Gln Phe Thr Ile Ile His Phe Ala Asn Gly 115 120 125 Gln Val Trp Leu Asn Leu Thr Val Asn His Ile Ile Ala Asp Gly Leu 130 135 140 Ser Val Asn Ala Leu Leu His Ala Val Met Glu Lys Tyr Leu Glu Leu 145 150 155 160 Arg Lys Gly Ile Ser Ser Ser Tyr Gln Pro Pro Ser Tyr Leu Asp Tyr 165 170 175 Ile Ser Ser Glu Arg Glu Tyr Glu Gln Ser Gln Arg Tyr Gln Lys Gly 180 185 190 Lys Glu Tyr Trp Leu Thr Lys Tyr Asn Thr Leu Pro Glu Thr Thr Gly 195 200 205 Ile Lys Ser Tyr Pro Pro Phe Ser Ile Gly Ser Glu Ser Asn Lys Leu 210 215 220 Ser Ile Thr Leu Asp Gly Ser Arg Tyr Glu Arg Ile Leu Ala Phe Cys 225 230 235 240 Glu Gln Tyr Gln Val Ser Leu Tyr Thr Leu Phe Leu Ser Ala Met Tyr 245 250 255 Ala Leu Leu Tyr Lys Leu Thr Asp Ser Thr Asp Val Pro Val Gly Thr 260 265 270 Val Phe Ala Asn Arg Thr Ser Lys Lys Glu Lys Glu Thr Ile Gly Met 275 280 285 Phe Val Ser Thr Val Ala Thr Arg Ile Cys Leu Asn Pro Asp Gly His 290 295 300 Val Leu Ser Leu Ile Gln Thr Val Ser Lys Glu Asn Thr Ala Asp Leu 305 310 315 320 Arg Tyr Gln Lys Tyr Pro Tyr Asn Gln Leu Ile Gln Asp Leu Arg Glu 325 330 335 Gln His Gly Arg Asn Asp Leu Ser Gly Leu Phe Arg Thr Ser Leu Glu 340 345 350 Tyr Leu Pro Leu Lys Ile Val Glu Tyr Glu Glu Ile Lys Val Arg Leu 355 360 365 Glu Ala His Phe Ala Arg His Glu Met Asp Asp Leu Leu Leu Arg Phe 370 375 380 Asp His Met Leu Asn Glu Ser His Val Ile Leu His Ala Ser Tyr Arg 385 390 395 400 Thr Gly Leu Phe Glu Lys Ala Glu Ile Asp Arg Ile Met Glu Gln Tyr 405 410 415 Val Thr Val Leu Asp Gln Phe Leu Gln Thr Pro Glu Leu Pro Val Arg 420 425 430 Glu Ile Ser Leu Leu Ser Asp Asp Glu Lys His Arg Ile Leu Asn Val 435 440 445 Phe Asn Pro Pro Val Ala Gly Leu Ser Glu Gly Glu Ala Phe His Arg 450 455 460 Tyr Val Glu Lys Phe Ala Arg Glu Ile Pro Asp His Pro Ala Val Val 465 470 475 480 Tyr Met Asp Lys Gln Leu Thr Tyr Ser Glu Leu Asn Glu Arg Ala Glu 485 490 495 Arg Leu Ala Ser Leu Leu Arg Glu Gln Gly Val Gly Arg Glu Thr Ile 500 505 510 Thr Gly Ile Trp Ala Glu Arg Ser Val Glu Leu Leu Val Gly Val Leu 515 520 525 Ala Val Trp Lys Ala Gly Gly Ala Tyr Val Pro Leu Asp Pro Asp Tyr 530 535 540 Pro Ala Glu Arg Ile Glu Tyr Met Leu Ser Asp Ser Asp Ala Ser Val 545 550 555 560 Leu Leu Thr Gln Arg His Leu Leu Glu Arg Ala Gly Gly Trp Leu Ala 565 570 575 Asp Asp Arg Leu Lys Leu Gln Ala Val Tyr Ala Met Asp Asp Glu Gln 580 585 590 Ile Tyr Asn Gly Asp Ala Leu Ala Val Glu Phe Glu Ser Ala Asp Ser 595 600 605 Ala Pro Gln Asp Leu Ala Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly 610 615 620 Arg Pro Lys Gly Val Met Ile Glu His Gly Ser Leu Val Asn Thr Ala 625 630 635 640 Asp Ala Tyr Arg Arg Glu Tyr Arg Leu Asp Gln Phe Pro Val Arg Leu 645 650 655 Leu Gln Leu Ala Ser Phe Ser Phe Asp Val Phe Val Gly Asp Ile Ala 660 665 670 Arg Thr Leu Tyr Asn Gly Gly Thr Met Val Ile Val Pro Lys Asp Asp 675 680 685 Arg Ile Asp Pro Asn Leu Leu Tyr Gly Trp Ile Arg Asp Gln Asn Ile 690 695 700 Thr Val Phe Glu Ser Thr Pro Ala Leu Ile Leu Pro Phe Met Gln His 705 710 715 720 Ile Tyr Glu Glu Gly Leu Asp Val Ser Ser Met Gln Leu Leu Ile Thr 725 730 735 Ser Ser Asp Ala Cys Ser Val Thr Asp Tyr Arg Leu Leu Gln Glu Arg 740 745 750 Phe Gly Gly Gln Phe Arg Ile Ile Asn Ser Tyr Gly Val Thr Glu Ala 755 760 765 Ala Ile Asp Ser Ser Phe Tyr Gly Glu Pro Leu Asp Lys Leu Pro Pro 770 775 780 Ser Gly His Val Pro Ile Gly Lys Ala Trp Leu Asn Ala Arg Phe Tyr 785 790 795 800 Ile Val Asp Ala Ala Leu Lys Pro Val Pro Val Gly Val Pro Gly Glu 805 810 815 Leu Val Ile Gly Gly Ala Gly Val Ala Arg Gly Tyr Trp Asn Arg Pro 820 825 830 Asp Leu Thr Ala Glu Lys Phe Ala Asp Ser Pro Phe Val Pro Gly Glu 835 840 845 Arg Leu Tyr Arg Thr Gly Asp Leu Ala Arg Trp Leu Glu Asp Gly Asn 850 855 860 Val Asp Phe Ile Gly Arg Ile Asp Tyr Gln Val Lys Ile Arg Gly Phe 865 870 875 880 Arg Ile Glu Leu Gly Glu Ile Glu Thr Ala Leu Leu Arg Phe Pro Gly 885 890 895 Val Lys Gln Ala Val Val Thr Asp Arg Thr Asp Glu Gln Gly Gln Lys 900 905 910 Tyr Leu Cys Gly Tyr Val Val Glu Asp Ala Ser Val Gln Leu Ser Asp 915 920 925 Leu Leu Ser Gln Leu Lys Gln Glu Leu Pro Ala His Met Val Pro Ala 930 935 940 Arg Leu Val Ser Leu Asp Lys Leu Pro Leu Thr Pro Asn Gly Lys Ile 945 950 955 960 Asp Arg Lys Ala Leu Pro Glu Pro Thr Gly Glu Val Glu Ala Gly His 965 970 975 Glu Tyr Val Ala Pro Arg Thr Thr Leu Glu Thr Arg Leu Ala Leu Ile 980 985 990 Trp Gln Gln Val Leu Gly Ile Ala Arg Val Gly Val Gln Asp Asp Phe 995 1000 1005 Phe Asp Leu Gly Gly His Ser Leu Arg Ala Ser Thr Leu Val Ser Lys 1010 1015 1020 Ile Arg Lys Glu Leu Gln Val Glu Val Pro Leu Arg Glu Val Phe Arg 1025 1030 1035 1040 Tyr Thr Thr Ile Glu Gln Leu Ala Gln Arg Ile Gly Gly Leu Arg Gln 1045 1050 1055 Gln Glu Thr Tyr Glu Ile Thr Lys Ala Ala Glu Ala Glu Tyr Tyr Pro 1060 1065 1070 Val Ser Ser Glu Gln Lys Arg Leu Tyr Val Leu Arg Gln Leu Asp Gly 1075 1080 1085 Ala Glu Arg Ser Tyr Asn Met Ser Ala Ala Leu Leu Leu Glu Gly Lys 1090 1095 1100 Leu Asp Arg Thr Arg Val Glu His Ala Phe Arg Ala Leu Ile Gln Arg 1105 1110 1115 1120 His Glu Thr Leu Arg Thr Gly Ile Glu Gln Val Gln Gly Glu Leu Val 1125 1130 1135 Gln Arg Ile Tyr Asp Glu Leu Asp Phe Ala Val Asp Tyr Phe Gln Ala 1140 1145 1150 Ser Glu Arg Glu Val Glu Gln Val Val Glu Ala Tyr Tyr His Pro Phe 1155 1160 1165 Asp Leu Thr Lys Pro Pro Leu Leu Arg Ile Gly Leu Ile Glu Val Ala 1170 1175 1180 Glu Asp Arg His Ile Leu Leu Phe Asp Met His His Ile Val Ser Asp 1185 1190 1195 1200 Gly Ile Ser Thr Ala Leu Leu Phe Asp Glu Phe Ser Arg Leu Tyr Arg 1205 1210 1215 Gly Glu Glu Leu Ala Pro Leu Arg Ile Gln Tyr Lys Asp Tyr Ala Val 1220 1225 1230 Trp Gln His Ser Glu Ala Tyr Gly Gln Met Leu Gln Pro Gln Lys Glu 1235 1240 1245 Tyr Trp Leu Glu Gln Leu Ser Gly Glu Leu Pro Val Leu Glu Leu Pro 1250 1255 1260 Thr Asp Phe Pro Arg Pro Ala Val Gln Ser Phe Asp Gly Arg Thr Val 1265 1270 1275 1280 Lys Phe Tyr Ile Glu Lys Asp Arg Thr Glu Lys Leu Lys Glu Leu Ala 1285 1290 1295 Ala Arg Thr Gly Thr Thr Leu Tyr Met Val Leu Leu Ser Ala Tyr Thr 1300 1305 1310 Ile Leu Met His Lys Tyr Ser Gly Gln Glu Asp Leu Ile Val Gly Thr 1315 1320 1325 Pro Ile Ala Gly Arg Thr Gln Glu Glu Val Gln Pro Ile Val Gly Met 1330 1335 1340 Phe Ile Asn Thr Leu Ala Ile Arg Ser Arg Pro Glu Arg Ser Lys Pro 1345 1350 1355 1360 Tyr Leu Ser Tyr Leu Glu Glu Ile Lys Asp Ile Thr Leu Gly Ala Phe 1365 1370 1375 Glu His Gln Asn Tyr Leu Phe Glu Asp Leu Val Glu Ser Leu His Ile 1380 1385 1390 Pro Arg Ala Thr Gly Arg Asn Pro Leu Phe Asp Thr Phe Phe Ser Leu 1395 1400 1405 Gln Asn Thr Glu Asn Glu Gln Ile Val Ile Glu Gly Leu Glu Gln Ser 1410 1415 1420 Phe Tyr Pro Leu Glu Asn Gln Thr Ser Lys Phe Glu Leu Leu Leu Asp 1425 1430 1435 1440 Ile Ser Glu Leu Asp Gly Gln Leu Glu Cys Arg Leu Glu Tyr Ala Thr 1445 1450 1455 Ala Leu Tyr Lys Gln Glu Thr Ala Glu Arg Phe Ala Arg His Tyr Asp 1460 1465 1470 Lys Leu Leu Glu Thr Ile Ala Ala Ala Pro Asp Gly Asp Ile Ala Ser 1475 1480 1485 Leu Glu Met Leu Thr Glu Glu Glu Ile Arg Glu Leu Met Arg Gly Phe 1490 1495 1500 Asn Asp Ser Glu Ala Asp Tyr Pro Arg Gln Gln Thr Ile His Gly Leu 1505 1510 1515 1520 Phe Glu Glu Gln Ala Glu Leu Tyr Pro Asp Asn Val Ala Ala Val Met 1525 1530 1535 Asn Glu Arg Gln Leu Thr Tyr Arg Glu Leu Asn Glu Arg Ser Asn Arg 1540 1545 1550 Leu Ala Arg Lys Leu Arg Glu Thr Gly Val Glu Ala Asp Gln Leu Val 1555 1560 1565 Ala Ile Leu Ala Glu Arg Ser Leu Asp Met Val Val Gly Ile Leu Ala 1570 1575 1580 Ile Leu Lys Ala Gly Gly Ala Tyr Val Pro Val Asp Pro Asp Tyr Pro 1585 1590 1595 1600 Glu Glu Cys Ile Arg Phe Met Ile Glu Asp Ser Gly Ala Pro Leu Leu 1605 1610 1615 Leu Ile Gln Lys His Leu His Glu Lys Thr Asp Phe Val Gly Thr Arg 1620 1625 1630 Leu Glu Leu Asp Asp Phe Val Trp Gly Asp Arg Gly Ala Asp Ser Glu 1635 1640 1645 Gly Ala Leu Asp Ala Ser Asn Leu Glu Pro Ile Ser Gly Pro Leu Asn 1650 1655 1660 Leu Ala Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly Arg Pro Lys Gly 1665 1670 1675 1680 Thr Leu Ile Glu His Lys Asn Val Val Arg Leu Leu Phe Asn Asp Lys 1685 1690 1695 Asn Leu Phe Asp Phe Gly Pro Ser Asp Thr Trp Thr Leu Phe His Ser 1700 1705 1710 Phe Cys Phe Asp Phe Ser Val Trp Glu Met Tyr Gly Ala Leu Leu Tyr 1715 1720 1725 Gly Gly Lys Leu Val Ile Val Pro Pro Phe Thr Ala Lys Asn Pro Ala 1730 1735 1740 Asp Phe Leu Ala Leu Leu Gly Arg Glu Gln Val Thr Ile Leu Asn Gln 1745 1750 1755 1760 Thr Pro Thr Tyr Phe Tyr Gln Leu Leu Arg Glu Val Leu Thr Asp His 1765 1770 1775 Pro Tyr Asp Leu Arg Ile Arg Asn Val Ile Phe Gly Gly Glu Ala Leu 1780 1785 1790 Ser Pro Leu Leu Leu Lys Gly Phe Lys Thr Lys Tyr Pro Glu Thr Lys 1795 1800 1805 Leu Ile Asn Met Tyr Gly Ile Thr Glu Thr Thr Val His Val Thr Tyr 1810 1815 1820 Lys Glu Ile Thr Trp Val Glu Met Glu Ala Ala Lys Ser Asn Ile Gly 1825 1830 1835 1840 Lys Pro Ile Pro Thr Leu Arg Val Tyr Val Leu Asp Glu Asn Arg Arg 1845 1850 1855 Pro Val Pro Ile Gly Val Ala Gly Glu Met Tyr Val Ala Gly Glu Gly 1860 1865 1870 Leu Ala Arg Gly Tyr Leu Asn Arg Pro Asp Leu Thr Ala Glu Lys Phe 1875 1880 1885 Val Asp Ser Pro Phe Ala Glu Gly Glu Lys Leu Tyr Arg Ser Gly Asp 1890 1895 1900 Leu Ala Ala Trp Leu Pro Asp Gly Asn Ile Glu Tyr Leu Gly Arg Ile 1905 1910 1915 1920 Asp His Gln Val Lys Ile Arg Gly Tyr Arg Ile Glu Leu Asp Glu Ile 1925 1930 1935 Glu Thr Gln Leu Leu Asn Val Glu Gly Val Glu Glu Ala Val Val Leu 1940 1945 1950 Ala Arg Gln Asp Gly Gly Gly Glu Lys Ala Leu Val Ala Tyr Phe Val 1955 1960 1965 Ala Asp Arg Thr Leu Thr Val Ser Glu Met Arg Thr Ser Leu Ala Gln 1970 1975 1980 Gly Met Pro Gly Tyr Met Ile Pro Ser Tyr Phe Val Gln Leu Glu Arg 1985 1990 1995 2000 Met Pro Leu Thr Ala Asn Gly Lys Val Asp Arg Lys Ala Leu Pro Glu 2005 2010 2015 Pro Gln Gly Ser Leu Gln Thr Gly Val Glu Tyr Val Ala Pro Arg Asn 2020 2025 2030 Arg Met Glu Ser Gln Leu Val Lys Ile Trp Glu Glu Val Leu Gly Tyr 2035 2040 2045 Ser Gly Ile Gly Val Leu Asp Asn Phe Phe Glu Leu Gly Gly His Ser 2050 2055 2060 Leu Arg Ala Thr Asn Leu Val Ser Lys Ile Gln Lys Glu Met Asn Val 2065 2070 2075 2080 Glu Leu Pro Leu Arg Asp Val Phe Arg Tyr Thr Thr Val Glu Ser Met 2085 2090 2095 Ala Gly Ala Ile Ala Ser Leu Glu Glu Thr Arg His Asn Ser Ile Pro 2100 2105 2110 Lys Ala Glu Glu Lys Ala Tyr Tyr Pro Val Ser Ser Ala Gln Lys Arg 2115 2120 2125 Leu Tyr Val Leu His Gln Leu Asp Gly Ser Glu Leu Asn Tyr Asn Leu 2130 2135 2140 Pro Ser Ala Leu Gln Leu Glu Gly Ala Leu Asn Glu Ala Lys Val Glu 2145 2150 2155 2160 Lys Ala Leu Thr Thr Leu Val Ala Arg His Asp Met Leu Arg Thr Gly 2165 2170 2175 Phe Glu Ile Val Asn Gly Glu Pro Val Gln Arg Ile His Pro Ile Val 2180 2185 2190 Ala Phe Lys Val Glu Lys Leu Gln Ala Ser Glu Asp Gln Val Ala Ala 2195 2200 2205 Ile Leu Glu Gly Phe Ile Gln Pro Phe Asp Leu Thr Gln Pro Pro Leu 2210 2215 2220 Leu Arg Ala Leu Leu Ile Glu Leu Glu Lys Glu Lys Phe Leu Leu Ala 2225 2230 2235 2240 Leu Asp Ile His His Ile Gly Ser Asp Gly Leu Ser Met Asp Val Leu 2245 2250 2255 Leu Arg Glu Phe Val Arg Leu Tyr Asn Gly Glu Glu Leu Pro Glu Leu 2260 2265 2270 Arg Ile Gln Tyr Lys Asp Tyr Ala Val Trp Gln Gln Ser Glu Glu Gln 2275 2280 2285 Arg Gln Arg Ile Lys Arg Gln Glu Glu Tyr Trp Arg Gly Val Phe Ser 2290 2295 2300 Ser Glu Leu Pro Val Leu Glu Leu Pro Leu Asp Phe Ser Arg Pro Ala 2305 2310 2315 2320 Val Gln Gln Phe Asp Gly Gln Thr Leu Thr Phe Thr Leu Asp Ala Glu 2325 2330 2335 Lys Ser Glu Ala Leu Lys Arg Leu Ala Gly Asp Ser Gly Ala Thr Leu 2340 2345 2350 Tyr Met Leu Leu Leu Ala Ala Tyr Ser Val Leu Leu His Lys Tyr Ala 2355 2360 2365 Gly Gln Glu Asp Ile Val Val Gly Thr Pro Ile Ala Ala Arg Ser His 2370 2375 2380 Ala Asp Leu Gln Pro Ile Ile Gly Met Phe Val Asn Thr Leu Ala Leu 2385 2390 2395 2400 Arg Leu Gly Pro Ala Ala Glu Arg Thr Phe Leu Asp Tyr Leu Gln Glu 2405 2410 2415 Val Lys Glu Thr Thr Leu Gly Ala Tyr Glu His Gln Asp Tyr Pro Phe 2420 2425 2430 Glu Glu Leu Val Glu Ala Leu Gln Val Ser Arg Asp Leu Ser Arg Asn 2435 2440 2445 Pro Leu Phe Asp Thr Met Phe Ser Leu Gln Lys His Glu Ser Leu Asp 2450 2455 2460 Leu Thr Leu Glu Gly Leu Gln Trp Ser Leu Phe Asp Ile Glu Glu Lys 2465 2470 2475 2480 Thr Ala Lys Phe Asp Leu Ser Phe Asp Ile Val Glu Ala Asp Asn Glu 2485 2490 2495 Leu Val Cys Lys Ile Glu Tyr Ala Thr Ser Leu Phe Arg Gln Glu Thr 2500 2505 2510 Val Val Arg Leu Ala Gly His Tyr Glu Gln Leu Leu Thr Ser Ile Leu 2515 2520 2525 Ala Gln Pro Gly Ala Arg Ile Ser Asp Leu Asp Val Leu Thr Asp Ser 2530 2535 2540 Glu Lys His Asp Leu Leu Val Gly Phe Asp Val Ser Ser Ser Ala Leu 2545 2550 2555 2560 Ala Lys Gln Pro Ala Ala Glu Gly Thr Gly Leu Glu Ala Asp Glu Ser 2565 2570 2575 Trp Arg Glu Arg Thr Phe His Glu Leu Phe Glu Glu Gln Ala Glu Arg 2580 2585 2590 Thr Pro Glu Ala Leu Ala Val Val Tyr Glu Asp Ser Lys Leu Thr Tyr 2595 2600 2605 Ala Glu Leu Asn Ala Lys Ala Asn Arg Leu Ala Tyr Ala Leu Arg Ala 2610 2615 2620 Arg Gly Val Lys Pro Glu Gln Val Val Gly Ile Leu Ala Asp Arg Ser 2625 2630 2635 2640 Ala Glu Leu Leu Ile Gly Val Leu Ala Val Trp Lys Ala Gly Gly Ala 2645 2650 2655 Tyr Val Pro Leu Asp Pro Asp Tyr Pro Ala Glu Arg Ile Glu Tyr Met 2660 2665 2670 Leu Ala Asp Ser Gly Ala Ser Val Leu Leu Thr Gln Thr Arg Leu Leu 2675 2680 2685 Glu Gln Ala Glu Ala Trp Arg Ser Asp Gly Ala Leu Val Leu Gln Thr 2690 2695 2700 Val Leu Ala Leu Asp Asp Val Ala Thr Tyr Ser Leu Gly Ala Ala Glu 2705 2710 2715 2720 Ala Ala Val Gly Val Gln Ala Leu Gly Glu Ala Gly Ala Glu Ala Glu 2725 2730 2735 Ala Leu Ala Gln Ala Ala Ala Ala Glu Thr Ser Ala Thr Ala Glu Ala 2740 2745 2750 Glu Lys Asn Val Leu Ala Ala Asp Leu Ala Ser Asn Pro Ala Asn Val 2755 2760 2765 Asn Lys Pro Arg Asp Leu Ala Tyr Val Ile Tyr Thr Ser Gly Thr Thr 2770 2775 2780 Gly Lys Pro Lys Gly Val Met Ile Glu His Arg Ser Leu Val Asn Thr 2785 2790 2795 2800 Ala Ala Gly Tyr Arg Arg Glu Tyr Arg Leu Asp Gln Phe Pro Val Arg 2805 2810 2815 Leu Leu Gln Leu Ala Ser Phe Ser Phe Asp Val Phe Val Gly Asp Ile 2820 2825 2830 Ala Arg Thr Leu Tyr Asn Gly Gly Thr Met Val Ile Val Pro Lys Asp 2835 2840 2845 Asp Arg Ile Asp Pro Ser Arg Leu His His Trp Met Glu Arg Glu Arg 2850 2855 2860 Val Thr Ile Phe Glu Ser Thr Pro Ala Leu Ile Val Pro Phe Leu Glu 2865 2870 2875 2880 Tyr Val His Glu Gln Arg Leu Asp Met Ser Gly Met Glu Leu Leu Ile 2885 2890 2895 Thr Ser Ser Asp Ser Cys Ser Val Ala Asp Tyr Arg Thr Leu Gln Glu 2900 2905 2910 Arg Phe Gly Ser Gln Phe Arg Ile Ile Asn Ser Tyr Gly Val Thr Glu 2915 2920 2925 Ala Ala Ile Asp Ser Ser Phe Tyr Asp Glu Pro Leu Glu Lys Leu Pro 2930 2935 2940 Lys Thr Gly Ser Val Pro Ile Gly Lys Ala Trp Leu Asn Ala Lys Phe 2945 2950 2955 2960 Tyr Ile Val Asp Ala Asn Leu Lys Pro Val Pro Ile Gly Val Leu Gly 2965 2970 2975 Glu Leu Val Ile Gly Gly Val Gly Val Ala Arg Gly Tyr Leu Asn Arg 2980 2985 2990 Pro Asp Leu Thr Ala Glu Lys Phe Val Asp Ser Pro Phe Ala Thr Gly 2995 3000 3005 Glu Arg Leu Tyr Arg Thr Gly Asp Leu Ala Arg Trp Met Ser Asp Gly 3010 3015 3020 Asn Val Asp Phe Ile Gly Arg Ile Asp Asn Gln Val Lys Ile Arg Gly 3025 3030 3035 3040 Tyr Arg Ile Glu Thr Gly Glu Val Glu Ala Lys Leu Leu Ser Val Gly 3045 3050 3055 Gly Val Lys Glu Ala Val Val Val Val Arg Glu Asp Gln Glu Gly Gln 3060 3065 3070 Lys Ala Leu Cys Ala Tyr Tyr Thr Ala Glu Glu Gly Leu Thr Ala Ala 3075 3080 3085 Asp Leu Lys Arg Ala Ile Ala Ser Glu Leu Pro Gly Tyr Met Ile Pro 3090 3095 3100 Ser Tyr Phe Val Glu Leu Glu Arg Leu Pro Leu Thr Pro Asn Gly Lys 3105 3110 3115 3120 Ile Asp Arg Lys Ala Leu Pro Ala Pro Glu Gly Gly Ala Gly Gly Arg 3125 3130 3135 Glu Tyr Val Ala Pro Arg Thr Glu Leu Glu Ala Lys Leu Ala Ala Ile 3140 3145 3150 Trp Gln Asp Val Leu Val Arg Glu Lys Ala Val Gly Val Thr Asp Asn 3155 3160 3165 Phe Phe Asp Leu Gly Gly His Ser Leu Arg Ala Thr Thr Leu Val Ser 3170 3175 3180 Lys Met His Lys Glu Leu Gly Val Glu Phe Pro Leu Arg Asp Val Phe 3185 3190 3195 3200 Arg Tyr Ser Thr Val Glu Glu Met Ala Ala Ala Met Glu Arg Leu Glu 3205 3210 3215 Ile Gly Ser Phe Thr Ala Ile Pro Ala Ala Glu Ser Ser Glu Tyr Tyr 3220 3225 3230 Pro Leu Ser Ser Ala Gln Lys Arg Leu Tyr Ile Leu Asn Gln Leu Glu 3235 3240 3245 Gly Ala Glu Leu Ser Tyr Asn Ile Pro Gly Ala Met Leu Leu Glu Gly 3250 3255 3260 Glu Leu Asp Arg Pro Arg Phe Glu Glu Ala Phe Arg Gly Leu Val Ala 3265 3270 3275 3280 Arg His Glu Thr Leu Arg Thr Gly Phe Glu Met Val Lys Gly Glu Ala 3285 3290 3295 Val Gln Arg Ile Tyr Glu Glu Ala Ala Phe Gln Val Glu Tyr Val Gln 3300 3305 3310 Ile Ser Glu Glu Gln Ala Glu Glu Thr Val Arg Gln Phe Val Arg Pro 3315 3320 3325 Phe Asp Leu Ala Lys Pro Pro Leu Leu Arg Val Gly Leu Ala Glu Leu 3330 3335 3340 Ala Pro Asp Arg His Ile Leu Met Phe Asp Thr His His Ile Val Ser 3345 3350 3355 3360 Asp Gly Val Ser Ile Asp Val Leu Ile Glu Glu Leu Val Arg Leu Tyr 3365 3370 3375 Ser Gly Glu Gln Leu Glu Pro Leu Arg Ile Gln Tyr Lys Asp Tyr Ala 3380 3385 3390 Val Trp Gln Gln Ser Asp Glu Gln Lys Ala Gln Leu Ala Lys Gln Glu 3395 3400 3405 Ala Tyr Trp Leu Asp Met Phe Arg Gly Glu Leu Pro Val Leu Glu Leu 3410 3415 3420 Pro Thr Asp Tyr Pro Arg Pro Ala Met Gln Ser Tyr Glu Gly Arg Thr 3425 3430 3435 3440 Leu Gln Leu Phe Met Asp Ser Glu Lys Ser Glu Gly Leu Lys Arg Leu 3445 3450 3455 Ala Ala Glu Asn Gly Ala Thr Leu Tyr Met Val Leu Leu Ala Gly Tyr 3460 3465 3470 Thr Ile Leu Leu His Lys Tyr Thr Gly Gln Glu Asp Val Val Val Gly 3475 3480 3485 Thr Pro Ile Ala Gly Arg Asn His Ser Asp Val Gln Pro Leu Ile Gly 3490 3495 3500 Met Phe Val Asn Thr Leu Ala Ile Arg Ser Tyr Pro Ala Ala Gly Lys 3505 3510 3515 3520 Thr Phe Leu Asp Tyr Leu Lys Glu Ile Lys Glu Thr Thr Leu Gly Ala 3525 3530 3535 Phe Glu His Gln Asn Tyr Pro Phe Glu Glu Leu Val Asp Lys Val Asn 3540 3545 3550 Val Ala Arg Asp Leu Ser Arg Asn Pro Leu Phe Asp Thr Met Phe Ala 3555 3560 3565 Leu Gln Asn Thr Glu Asn Leu Glu Ile Gln Leu Pro Gly Leu His Leu 3570 3575 3580 Ser Thr Tyr Ala Ser Glu Glu Ile Val Ser Lys Phe Asp Leu Ser Leu 3585 3590 3595 3600 Asp Val Thr Glu Ile Glu Glu Gly Leu Glu Tyr Leu Phe Glu Tyr Ala 3605 3610 3615 Thr Ala Leu Tyr Lys Thr Glu Thr Val Glu Lys Leu Ala Ala His Tyr 3620 3625 3630 Leu Gln Leu Leu Glu Ser Ile Leu Arg Asn Pro Ser Ala Thr Ile Ala 3635 3640 3645 Glu Leu Gly Ile Leu Thr Pro Ala Glu Lys Glu Gln Ile Leu Gly Ala 3650 3655 3660 Phe Asn Pro Ala Gln Pro Glu Ala Ala Pro Ala Ala Ala Phe His Arg 3665 3670 3675 3680 Leu Phe Glu Glu Gln Ala Glu Arg Thr Pro Glu Ala Ala Ala Val Val 3685 3690 3695 Tyr Glu Ser Asp Arg Leu Thr Tyr Ala Glu Leu Asn Glu Arg Ala Asn 3700 3705 3710 Arg Leu Ala Ala Thr Val Arg Ala Ser Gly Ile Gly Arg Asp Thr Ile 3715 3720 3725 Val Gly Ile Leu Ala Glu Arg Ser Val Asp Leu Leu Val Ala Val Leu 3730 3735 3740 Ala Val Trp Lys Ala Gly Gly Ala Tyr Val Pro Leu Asp Pro Asp Tyr 3745 3750 3755 3760 Pro Ala Glu Arg Val Arg Phe Met Leu Glu Asp Ser Gly Ala Lys Val 3765 3770 3775 Leu Leu Thr Gln Thr Leu Leu Arg Glu Arg Ala Glu Ala Trp Leu Gly 3780 3785 3790 Glu Glu Glu Leu Ala Leu Ala Ala Val Leu Tyr Leu Asp Asp Glu Ala 3795 3800 3805 Ser Tyr Ser Glu Glu Arg Ala Asn Ala Pro Leu Glu Ala Asp Asp His 3810 3815 3820 Leu His Glu Cys Arg Pro Glu Asp Leu Ala Tyr Val Ile Tyr Thr Ser 3825 3830 3835 3840 Gly Thr Thr Gly Lys Pro Lys Gly Val Met Ile Glu His Arg Ser Leu 3845 3850 3855 Val Asn Thr Ala Ala Gly Tyr Arg Arg Glu Tyr Gln Leu Asn Gln Phe 3860 3865 3870 Pro Val Arg Leu Leu Gln Leu Ala Ser Phe Ser Phe Asp Val Phe Val 3875 3880 3885 Gly Asp Ile Ala Arg Thr Leu Tyr Asn Gly Gly Thr Met Val Ile Val 3890 3895 3900 Pro Lys Asp Asp Arg Ile Asn Pro Ser Arg Leu His Tyr Trp Ile Ser 3905 3910 3915 3920 Gln Gln Gln Val Thr Ile Phe Glu Ser Thr Pro Ala Leu Ile Val Pro 3925 3930 3935 Phe Leu Glu Tyr Val His Glu His Gln Leu Asp Met Ser Gly Met Glu 3940 3945 3950 Leu Leu Ile Thr Ser Ser Asp Ser Cys Ser Val Ala Asp Tyr Arg Thr 3955 3960 3965 Leu Gln Glu Arg Phe Gly Ser Leu Phe Arg Ile Ile Asn Ala Tyr Gly 3970 3975 3980 Val Thr Glu Ala Ala Ile Asp Ser Ser Phe Tyr Asp Glu Glu Leu Ala 3985 3990 3995 4000 Lys Leu Pro Gln Thr Gly Asn Val Pro Ile Gly Lys Ala Trp Leu Asn 4005 4010 4015 Ala Lys Phe Tyr Ile Val Asp Ala His Leu Asn Pro Val Pro Val Gly 4020 4025 4030 Val Leu Gly Glu Leu Val Ile Gly Gly Val Gly Val Ala Arg Gly Tyr 4035 4040 4045 Leu Asn Arg Pro Glu Leu Thr Glu Glu Lys Phe Val Asp Ser Pro Phe 4050 4055 4060 Ala Ala Gly Glu Arg Leu Tyr Arg Thr Gly Asp Leu Ala Arg Trp Met 4065 4070 4075 4080 Glu Asp Gly Asn Val Asp Phe Ile Gly Arg Ile Asp Asn Gln Val Lys 4085 4090 4095 Ile Arg Gly Tyr Arg Ile Glu Thr Gly Glu Val Glu Ala Lys Leu Leu 4100 4105 4110 Ser Val Gly Gly Val Lys Glu Ala Val Val Val Val Arg Glu Asp Gln 4115 4120 4125 Glu Gly Gln Lys Ala Leu Cys Ala Tyr Tyr Thr Ala Glu Glu Asp Leu 4130 4135 4140 Thr Ala Ala Asp Leu Lys Arg Ala Ile Ala Ser Glu Leu Pro Gly Tyr 4145 4150 4155 4160 Met Ile Pro Ser Tyr Phe Val Glu Leu Glu Arg Leu Pro Leu Thr Pro 4165 4170 4175 Asn Gly Lys Ile Asp Arg Lys Ala Leu Pro Ala Pro Glu Gly Gly Ala 4180 4185 4190 Gly Gly Gly Arg Glu Tyr Val Ala Pro Arg Thr Glu Leu Glu Ala Lys 4195 4200 4205 Leu Ala Ala Ile Trp Gln Asp Val Leu Val Arg Glu Lys Ala Val Gly 4210 4215 4220 Val Thr Asp Asn Phe Phe Asp Leu Gly Gly His Ser Leu Arg Ala Thr 4225 4230 4235 4240 Thr Leu Val Ser Lys Met His Lys Glu Leu Gly Val Glu Phe Pro Leu 4245 4250 4255 Arg Asp Val Phe Arg Tyr Ser Thr Val Glu Glu Met Ala Thr Ala Met 4260 4265 4270 Glu Arg Leu Glu Ile Gly Ser Phe Thr Ala Ile Pro Ala Ala Glu Pro 4275 4280 4285 Ser Glu Tyr Tyr Pro Leu Ser Ser Ala Gln Lys Arg Leu Tyr Ile Leu 4290 4295 4300 Asn Gln Leu Glu Gly Ala Glu Leu Ser Tyr Asn Ile Pro Gly Ala Met 4305 4310 4315 4320 Leu Leu Glu Gly Glu Leu Asp Arg Gln Arg Phe Glu Glu Ala Phe His 4325 4330 4335 Gly Leu Val Ala Arg His Glu Thr Leu Arg Thr Gly Phe Glu Met Val 4340 4345 4350 Lys Gly Glu Ala Val Gln Arg Ile Tyr Glu Glu Ala Ala Phe Gln Val 4355 4360 4365 Glu Tyr Val Gln Ile Ser Gly Glu Gln Ala Glu Glu Thr Val Arg Gln 4370 4375 4380 Phe Val Arg Pro Phe Asp Leu Ala Lys Pro Pro Leu Leu Arg Val Gly 4385 4390 4395 4400 Leu Ala Glu Leu Ala Pro Asp Arg His Ile Leu Met Phe Asp Thr His 4405 4410 4415 His Ile Val Ser Asp Gly Val Ser Ile Asp Val Leu Ile Glu Glu Phe 4420 4425 4430 Val Arg Leu Tyr Ser Gly Glu Gln Leu Glu Pro Leu Arg Ile Gln Tyr 4435 4440 4445 Lys Asp Tyr Ala Val Trp Gln Gln Ser Asp Glu Gln Lys Ala Gln Leu 4450 4455 4460 Ala Lys Gln Glu Ala Tyr Trp Leu Asp Met Phe Arg Gly Glu Leu Pro 4465 4470 4475 4480 Val Leu Glu Leu Pro Thr Asp Tyr Pro Arg Pro Ala Met Gln Ser Tyr 4485 4490 4495 Glu Gly Arg Thr Leu Gln Leu Phe Met Asp Ser Glu Lys Ser Glu Gly 4500 4505 4510 Leu Lys Arg Leu Ala Ala Glu Asn Gly Ala Thr Leu Tyr Met Val Leu 4515 4520 4525 Leu Ala Gly Tyr Thr Met Leu Leu His Lys Tyr Thr Gly Gln Glu Asp 4530 4535 4540 Val Val Val Gly Thr Pro Ile Ala Gly Arg Asn His Ser Asp Val Gln 4545 4550 4555 4560 Pro Leu Ile Gly Met Phe Val Asn Thr Leu Ala Ile Arg Ser Tyr Pro 4565 4570 4575 Ala Ala Gly Lys Thr Phe Leu Asp Tyr Leu Lys Glu Ile Lys Glu Thr 4580 4585 4590 Thr Leu Gly Ala Phe Glu His Gln Asn Tyr Pro Phe Glu Glu Leu Val 4595 4600 4605 Asp Lys Val Asn Val Ala Arg Asp Leu Ser Arg Asn Pro Leu Phe Asp 4610 4615 4620 Thr Met Phe Ala Leu Gln Asn Thr Glu Asn Leu Glu Ile Gln Leu Pro 4625 4630 4635 4640 Gly Leu His Leu Ser Thr Tyr Ala Ser Glu Glu Ile Val Ser Lys Phe 4645 4650 4655 Asp Leu Ser Leu Asp Val Thr Glu Ile Glu Glu Gly Leu Glu Tyr Leu 4660 4665 4670 Phe Glu Tyr Ala Thr Ala Leu Tyr Lys Thr Glu Thr Val Glu Lys Leu 4675 4680 4685 Ala Ala His Tyr Leu Gln Leu Leu Glu Ser Ile Leu Arg Asn Pro Ser 4690 4695 4700 Ala Thr Ile Ala Glu Leu Gly Ile Leu Thr Pro Ser Glu Lys Glu Gln 4705 4710 4715 4720 Ile Leu Gly Ala Phe Asn Pro Ala Gln Pro Glu Ala Ala Pro Ala Ala 4725 4730 4735 Ala Phe His Arg Leu Phe Glu Glu Gln Ala Glu Arg Thr Pro Glu Ala 4740 4745 4750 Ala Ala Val Val Tyr Glu Ser Asp Arg Leu Thr Tyr Ala Glu Leu Asn 4755 4760 4765 Glu Arg Ala Asn Arg Leu Ala Val Thr Leu Arg Thr Ser Gly Ile Gly 4770 4775 4780 Arg Asp Thr Ile Val Gly Ile Leu Ala Glu Arg Ser Val Asp Leu Leu 4785 4790 4795 4800 Val Ala Val Leu Ala Val Trp Lys Ala Gly Gly Ala Tyr Val Pro Leu 4805 4810 4815 Asp Pro Asp Tyr Pro Ala Glu Arg Val Arg Phe Met Leu Glu Asp Ser 4820 4825 4830 Gly Ala Lys Val Leu Leu Thr Gln Thr Leu Leu Arg Glu Arg Ala Glu 4835 4840 4845 Ala Trp Leu Gly Glu Glu Glu Leu Ala Leu Ala Ala Val Leu Tyr Leu 4850 4855 4860 Asp Asp Glu Ala Ser Tyr Ser Glu Glu Arg Ala Asn Ala Pro Leu Glu 4865 4870 4875 4880 Ala Asp Asp His Leu His Glu Cys Arg Pro Glu Asp Leu Ala Tyr Val 4885 4890 4895 Ile Tyr Thr Ser Gly Thr Thr Gly Lys Pro Lys Gly Val Met Ile Glu 4900 4905 4910 His Arg Ser Leu Val Asn Thr Ala Ala Gly Tyr Arg Arg Glu Tyr Gln 4915 4920 4925 Leu Asn Gln Phe Pro Val Arg Leu Leu Gln Leu Ala Ser Phe Ser Phe 4930 4935 4940 Asp Val Phe Val Gly Asp Ile Ala Arg Thr Leu Tyr Asn Gly Gly Thr 4945 4950 4955 4960 Met Val Ile Val Pro Lys Asp Asp Arg Ile Asp Pro Ser Arg Leu His 4965 4970 4975 His Trp Ile Glu Gln Glu Arg Val Thr Ile Phe Glu Ser Thr Pro Ala 4980 4985 4990 Leu Ile Val Pro Phe Leu Glu Tyr Val His Glu His Arg Leu Asp Met 4995 5000 5005 Ser Trp Met Glu Leu Leu Ile Thr Ser Ser Asp Ser Cys Ser Val Ala 5010 5015 5020 Asp Tyr Arg Thr Leu Gln Glu Arg Phe Gly Ser Leu Phe Arg Ile Ile 5025 5030 5035 5040 Asn Ala Tyr Gly Val Thr Glu Ala Ala Ile Asp Ser Ser Phe Tyr Asp 5045 5050 5055 Glu Glu Leu Ala Lys Leu Pro Gln Thr Gly Asn Val Pro Ile Gly Lys 5060 5065 5070 Ala Trp Leu Asn Ala Lys Phe Tyr Ile Val Asp Ala His Leu Asn Pro 5075 5080 5085 Val Pro Val Gly Val Leu Gly Glu Leu Val Ile Gly Gly Val Gly Val 5090 5095 5100 Ala Arg Gly Tyr Leu Asn Arg Pro Glu Leu Thr Glu Glu Lys Phe Val 5105 5110 5115 5120 Asp Ser Pro Phe Ala Ala Gly Glu Arg Leu Tyr Arg Thr Gly Asp Leu 5125 5130 5135 Ala Arg Trp Met Glu Asp Gly Asn Val Asp Phe Ile Gly Arg Ile Asp 5140 5145 5150 Asn Gln Ala Lys Ile Arg Gly Tyr Arg Ile Glu Thr Gly Glu Ile Glu 5155 5160 5165 Ser Gln Leu Leu Arg Val Glu Gly Val Arg Glu Ala Val Val Leu Val 5170 5175 5180 Arg Ser Asp Ala Asn Gly Gln Lys Ala Leu Cys Ala Tyr Tyr Thr Thr 5185 5190 5195 5200 Asp Gly Glu Leu Thr Val Ala Asp Leu Lys Arg Ala Ile Ser Ser Glu 5205 5210 5215 Leu Pro Gly Tyr Met Ile Pro Ser Tyr Phe Val Glu Leu Glu Arg Leu 5220 5225 5230 Pro Leu Thr Pro Asn Gly Lys Ile Asp Arg Lys Ala Leu Pro Ala Pro 5235 5240 5245 Glu Gly Gly Ala Ser Ala Gly Arg Glu Tyr Met Ala Pro Arg Thr Glu 5250 5255 5260 Leu Glu Ala Lys Leu Val Ala Ile Trp Gln Asp Val Leu Gly Pro Val 5265 5270 5275 5280 Thr Ile Gly Val Thr Asp Asn Phe Phe Asp Leu Gly Gly His Ser Leu 5285 5290 5295 Arg Ala Thr Thr Leu Val Ser Lys Val His Lys Glu Leu Ser Val Asp 5300 5305 5310 Leu Pro Leu Arg Glu Val Phe Arg His Ser Thr Ile Glu Ala Met Ala 5315 5320 5325 Glu Ala Ile Ser Gln Leu Glu Arg Gln Glu His Leu Ser Ile Pro Val 5330 5335 5340 Leu Asp Lys Arg Asp Tyr Tyr Pro Leu Ser Ser Val Gln Lys Arg Leu 5345 5350 5355 5360 Tyr Ile Gln Gln Gln Met Glu Gly Ala Glu Leu Ser Tyr Asn Met Ser 5365 5370 5375 Gly Met Thr Val Leu Val Gly Arg Leu Glu Arg Asn Gln Phe Glu Ala 5380 5385 5390 Ala Leu Lys Gly Leu Ile Ala Arg His Glu Ile Leu Arg Thr Gly Phe 5395 5400 5405 Glu Met Val Asp Gly Glu Pro Val Gln Arg Ile Tyr Pro Asn Leu Lys 5410 5415 5420 Phe Ala Val Glu Tyr Thr Lys Ala Thr Glu Ser Glu Thr Lys Ser Ile 5425 5430 5435 5440 Val Asp Gly Phe Val Arg Val Phe Asp Leu Glu Arg Pro Pro Leu Leu 5445 5450 5455 Arg Val Gly Leu Val Glu Trp Glu Ala Glu Arg His Leu Leu Met Leu 5460 5465 5470 Asp Ile His His Ile Val Thr Asp Gly Met Ser Met Gly Ile Phe Val 5475 5480 5485 Glu Glu Leu Leu Arg Leu Tyr Asn Gly Glu Thr Leu Glu Pro Leu Arg 5490 5495 5500 Ile Gln Tyr Lys Glu Phe Ala Ala Trp Gln Gln Ser Glu Pro Val Lys 5505 5510 5515 5520 Glu Arg Leu Lys Arg Gln Glu Ala Tyr Trp Leu Asp Val Leu Glu Gly 5525 5530 5535 Glu Leu Pro Thr Leu Glu Leu Pro Thr Asp Phe Val Arg Pro Ala Ala 5540 5545 5550 Arg Ser Phe Glu Gly Asp Val Leu Pro Phe Ser Ile Asp Lys Gln Met 5555 5560 5565 Thr Asp Ser Leu Gln Arg Ile Ala Asp Lys Asn Gly Ala Thr Leu Tyr 5570 5575 5580 Met Val Leu Leu Ala Ala Tyr Ser Ile Leu Leu Ser Lys Tyr Ser Gly 5585 5590 5595 5600 Gln Glu Asp Phe Ile Val Gly Met Pro Val Ser Gly Arg Thr His Ala 5605 5610 5615 Asp Leu Glu Pro Leu Ile Gly Met Phe Val Asn Thr Leu Ala Ile Arg 5620 5625 5630 His Tyr Pro Ser Gly Glu Lys Ala Phe Leu Ala Tyr Leu Asn Glu Val 5635 5640 5645 Lys Glu Thr Met Leu Gly Ala Tyr Asp His Gln Asp Tyr Pro Phe Glu 5650 5655 5660 Glu Leu Val Lys Lys Leu Gln Ala Pro Arg Asp Gln Ser Arg Asn Pro 5665 5670 5675 5680 Val Phe Asp Val Met Phe Ala Leu Glu Thr Lys Glu Asp Asn Val Gln 5685 5690 5695 Ser Phe Gly Asp Ile Lys Ile Glu Ser Tyr Pro Glu Thr His Thr Val 5700 5705 5710 Ser Gln Phe Asp Leu Thr Leu Val Ile Ser Leu Leu Asp Glu Gly Met 5715 5720 5725 Asn Gly Gln Phe Glu Tyr Ala Thr Lys Leu Phe Thr Arg Asn Leu Ile 5730 5735 5740 Asp Asn Phe Ala Gln Asp Leu Leu Met Ile Ile Thr Gln Ile Cys Glu 5745 5750 5755 5760 Gln Pro Ser Val Leu Leu Lys Asp Ile Ser Leu Asn Gly Gln Ser Glu 5765 5770 5775 Gln Glu Gln Asp Val Leu Glu Ala Ile Asp Ile Ile Phe 5780 5785 <210> 7 <211> 14880 <212> DNA <213> Paenibacillus polymyxa F4 <400> 7 atggcttttg aaaaagaaac gttgttttgg aacgaaaaat tcggtagcga cgattatacc 60 ttgacgcggc tgccttacag caaagctcca agctccctgg cgcccattat gacaaccgtc 120 ggcgggacgc tttcggagga agtggcgcag cgcgtccttc aaatgagcaa gggcgctccg 180 ctggccactt ttatgatttt gctcgctggt gttcagtcgc tcttgcataa atatacaggc 240 gcttctgaca ttctggtcgg catgccggtt gtacggaaac cgacggagac gcgccgatcc 300 gttaatcata cgatcatttt gaaaaactcg ctttcggcgg gtgcgacttt taaaacgctt 360 ctgagcgagc tgaggacttc cttgccggaa gcgattcagc atcaacatat tccgttcttg 420 aaaatgacgg agaagctgga cctgcaatat gcaggcggga tacccgtcgt ccatacgcta 480 gtatccctta aggagctgca tctggacgaa atcgggcaaa acgtggtcac agattgttcc 540 tttgaattca gcttaaccgg cgggacgata cagctagcgc tgtcatataa cgagcactta 600 tatgactccg agttcatgac tcgggtcgtc ggccatctga atcgcctgct ggccgtgggg 660 cttcacgagt tggagctgga catcgtgcgg gcggacatgc tgtcggagga cgagaaattt 720 caattgctgc aaagctttaa cgataccgag aaggactatc cccgtgatcg gacgattcat 780 cagctcgtgg aggagcaggc gaagcgggtg cccgaagcga cggcggttgt ctttgagggg 840 cggcggcttt cgtacgcgga gctgaacgaa cgggcgaacc ggctggcgcg gacgctgcga 900 tcggtcggcg tgctgcccaa tcagctggta ggcatgatgg ccaggagatc gctggagacg 960 gtcgttggca ttctagcggt tttgaaagct ggcggtgcct acgtgccgat cgacccggaa 1020 tacccggaag aacgcatccg ctacattctg gagaactcga acgcgcagct gctgctgact 1080 caaagggagt tgctgcagca ggtgccgttc gaagggaccg tggtggcgct ggatgacgag 1140 caggcctaca gcgacgatgg ctcgaacctg gagccggcca gcggtccgaa cgatctggct 1200 tatgtcatct atacatcagg tacgacgggc aaacccaaag gggtcatgct ggagcatcgc 1260 gggctggtca gcctgaaact gacgtttgcc catacgcttc acacgaccga acaggatcga 1320 gtgctgcaat tcgccagcct atcgttcgat gcgtcgtgct gggagatgtt caacgctctg 1380 tattttgggg cgaccttata tataccgtca accgagacga ttttggatga tcagctgttc 1440 gaacgtttca tgaacgagca tgcaattacg atcgccactt tgccgccaac ttatgcggct 1500 tatttgaatt cagaccgcct gccgagtctg agcaggctca tcacggcggg ctccgccgtt 1560 tcagccgaat tcgtgcagca gtggaaagac aaggtacagt actataatgc gtacggtccg 1620 acggaagcct cgatcgccac atccgtgtgg gcggcgtcta cgcatgacac cgaacgcaga 1680 gcgataccga tcggacgtcc gatctggaac catcgcttgt atatactggg cgcacagaat 1740 cagctggcgc ctatcggagt ggagggagaa ttatgcatcg cgggcgtcgg cctcgcccgg 1800 ggatattgga accggccgga tctgacggcg gagaaattca tcgaacaccc gtttgagccc 1860 ggagagagac tgtaccggac gggcgacttg gcccgctggc tgccggacgg aaatattgag 1920 tacttgggcc ggattgacca tcaggtgaaa atccgtggat tccggatcga gatcggcgag 1980 attgaagagc agcttctgaa gatcgactcc gtgcaggaga cgatcgtaat cgcgcgggaa 2040 ggcaaaagcg ggcaagaact gtgcgcttac ctggtcgcgg ggcgcccgct tacgctcggc 2100 gagctaagaa gcgcgctggc gcaaaaattg ccgaattaca tgattccggc gcattttgtt 2160 cagcttccgc agatgccgct cacgccgaac gacaaaatcg accgcaaggc tttgcccgct 2220 ccggaaggaa acgcgctgac cggcggcgcg tacgtagctc cccgcaatga agccgagcgg 2280 acgcttgccg atgtgtggca ggcggtattg aacgccgatc gcgttggggt aacggatcat 2340 ttcttcgagc tgggcggaga ctcgatcaag tccattcaag tatcttcgcg gcttcatcaa 2400 gccgggtaca agctggaaat ccgggatttg ttcaaatatc cgactatttc acagctcagc 2460 ctgcatgtga aaccgatcgg acgtaccatt gatcaaggcg aaataacggg tgaaacggcg 2520 ctgatgccga ttcagcattg gtttttcgag agctcctttg cggacccgca tcatttcaac 2580 cagtcggtga tgctgtaccg gaaggaacgc ttcgacgaag agacggtgcg tcaggtgctg 2640 caaaagctgg ccgagcatca tgacgccttg cggatggtgt tccgcaagac ggaacaaggg 2700 tttagcgcgc ggaaccgcgc gattcaggaa ggcgggctgt tcacgctgga cgtgttcgac 2760 ttcaaggatg cggagaatac cgcgcaggct gtggaagcga aggcaacgga cattcaagcg 2820 ggcatcgatc tggagaacgg gcccctcgcg aaggcgggac tgttccggtg cgcggacggc 2880 gatcatttgc tgctcgcggt tcatcatgcc gtggtggacg gcgtgtcttg gcgcattttg 2940 atggaggatt tcgctctggg ttacgagcag gccggcaaaa gcgaggaaat tcgtttcccg 3000 gcgaaaacgg atgcgtaccg cacttggtcc gagcagttgg ccgcttacgc gcaaagcccg 3060 gagatggcga aggaacgggc ctattggcag gccgtggaac aaattgcggt tccggccgtg 3120 ccgaaggatc tggaggcgga cgttacgacg cagcaggaca gcgaatcgct gttcgtccgt 3180 ttgactcccg aagaaacgga gctgctgctg aagcgggttc accgggccta caacaccgaa 3240 atgaacgata ttttggtaac ggcgctcggc atagccgttc gcaagtggac gggacacgaa 3300 cgggtgcgga tcaatctcga aggacacgga cgcgaatcga tcggaacgga tatcgacatc 3360 acgcgcacag tcggctggtt tacgaccaag tttccggtcg tcctggagcc ggaaaccgac 3420 cgggatttgg cctatcagat taaacaggtc aaggaaagct tgcgccgcat tccgaacaag 3480 gggcttgggt acggcgtatg ccgctatctc tccaaatcgg aggatggctt tgtttggggc 3540 gcagagccgg aaattaattt taactacctc ggccagttcg gcgatgatgt caatcaggac 3600 gagatcggca tatcttctta ttccagcggc agcccggcca gcgaccggca ggcccgcagc 3660 tttgtgctgg atatcaacgg catggtgctg gacggcgctt tatcgctcga tctcagctac 3720 agccggaagc agtatcgcaa ggaaacgatg gaagccttcg ctcagcggct tgagcaaagt 3780 ctccgagagc tcattaccca ctgcgcaggc aaagaaaaca ccgaattgac gccgagcgac 3840 gtgcaattta aaggcttgac catcgcggaa ttggagcaaa tcgcccagcg ctcgggccat 3900 ctcggggaaa tcgaaaatat ttactcgctt acgccgatgc agaagggcat gtggttccac 3960 agcgcgcttg accggcaaac ggccgcttac ttcgagcaga cgcggtttac gatgcgggga 4020 gcgctcgacg tccagctttt cgagaggagc tggatggagc ttgcgaaacg tcatctggtg 4080 ctgcgggcga attttgtgaa aggaccggcg ggcgagccgc tgcaaatcat ataccgcgac 4140 aagccggtcg gctttgaata tgaagagctg cttcatttgc aggcggacga gaaacaagcg 4200 tatttggata aaaaggcgga ggatgacaag cttcgcggct tcgacatgga acatgacgcg 4260 ctcgttcggg ttacgatcct acgcaccgaa gagcaaagct atcatgtgct gtggagtttc 4320 cagcatattt tgatggacgg ctggtgcctg ccgcagctga cgcaggagct gtttgagacg 4380 tactcggcct tggcatccgg caagcagcca gcgggagata agggatcgga ttatggcgct 4440 tatatcgaat ggttggagaa acaggacgat caggcggcat ccggctattg gacggcattc 4500 ctggcaggtt atgaagggca aaccgtactc ccgggacaaa aggaaccggc gccaaacggc 4560 agatttacgg ctgatcacgt caccgccgag ctgggcaagg acttgagcga gcggatggac 4620 cgggtggcga aacagcgcct ggttacggtc aatacgctgc tgcaagccgc ttggggcgtg 4680 atgctgcaaa aatataacgg aacaaacgat gccgtattcg gcagcgtcgt ggccggaaga 4740 ccggcggaaa tcccgggcat cgagtccatg atcgggctgt tcatcaatac ggtgccggtc 4800 cgcgtcacga gcgaagcgga caccgtgttc gccgacctga tggcgaagct ccaagagcgg 4860 gcgctggagt ccgggcgtta tgattactat ccgctgtatg aaattcaagc ccgcagcgtg 4920 caaaagcaga acctgatcaa ccatatcatc gctttcgaga actatccggt ggacgagcag 4980 atggagcagg cgggcgacca gcagcacggc gacctgacga tcgctgacgt tcagatggag 5040 gagcagacga actataactt caacgtgacc gtggtgccgg gagtcgagat cgaaattcga 5100 ttcgacttta acgccgaagt gttcgataaa gacagcatcg agcggctcaa ggggcatctc 5160 gtccatctgc tggagcaggt gacggataac ccggaaatta ccgtgggcga gctggaactt 5220 gtgacggagg cggaaaaggc cgaccttctc ggacgtttta acgacaccac cacggaattt 5280 ccgcgcggga agacgctcat tcaattgttc gaagagcagg cggagcgcat cccggatgca 5340 gccgctatct ccttgaatga gcaagagctg acctaccgcg agctgaacga acgcgtcaac 5400 cgccttgccc gtaccttgcg tagccacggg atatccaaag gccgtctggt cgccattatg 5460 gctgagcgtt ccatcgaaat ggtggtgggc atgctggcgg cacacaaagc cggagcggct 5520 tacgtaccga ttgacccgga atatcccgag gagcgtatcc gtttcttgat tgaggattcg 5580 ggagcgcagg tcatgctgac gcaaagccgc ttgcgcgagc gcctggcggg ttcggactcc 5640 gtgatcttac tggatgacga gtccttctat cacgaggacg gcacaaatct aaatccgggc 5700 accgaagcga cggatctggc ctgcgtcatc tatacgtcag gcacgacggg caagccgaaa 5760 ggcaaccttg tttcgcaccg caacatcgtg cggatcgtgc gaaatacgaa ttatatcgac 5820 atcaccgaac gggatcatgt cctccagctt tcgagctatt cgttcgacgg agcgattttc 5880 gatattttcg gcgctttgac caacggggcg cggctggtgc tggttccccg cgagactttg 5940 ctggaaatcg tccggctggc ggatctcatc cagcgtgagc gcatctcggt catgctgatt 6000 acgacggctt tcttcaacgt ccttgtagat gtgaacgtcg actgcctgcg ggatgtccgg 6060 gcgattttgt tcggaggaga gcgagtgtcg gtcggacatg tgcgtaaagc gctcgcccat 6120 atcggaccgg gcaggctcaa ccacctgtac ggcccgtcgg aaagcacggt ttataccacg 6180 taccttccgg tcgacttcgt cgatgagtcg gcggttactg tacccatcgg acggccgatc 6240 agtaatacga aggtgtatat cgtcgacagc cggaataagc ttctgccgat cggcgtggcc 6300 ggggagcttt gcgtcggcgg agaaggcttg gtacggggct acaataaccg gccggagctg 6360 acggcggaga aatttgtgga caatccgttt gtgctgggag agcgcatgta ccggacgggg 6420 gatttggcga aatggctgcc ggacggcacg atcgaatacg tgggacggac ggacgaccaa 6480 gtgaaaatcc gcggcttccg tattgagctg ggcgagatcg aagctcagct tcagaaagtg 6540 gagggaattc ggaaagcgac ggtattcgcg agggaagacg cctccggcga gaagcagctt 6600 tgcgcttatt atgaagcgga ccgcgagctt ccggcggccg agctgaagag cgtgctttcc 6660 caggaactgc cggcctatat gattccggcg tacctgatcc agttggagca gctcccgctg 6720 acgacgaacg gcaaggtcga ccgccgatca ctcccggcgc cggaggagag cttgcagccg 6780 ggcgaagaac gtactccgcc tcggactccg ctggaagcca gcttggcggg aatttggaaa 6840 agcgtgctcg gactggagca catcggggtt catgacaact tcttcgacct gggcggccat 6900 tccctgcggg cgacgacact ggtgagcaag gtgcatcagg agctgaacgt cgagctgcct 6960 ctgcgcgacg tattccgcta ctcgacgatc gaagagatgg ctctcgccat ctcccggatc 7020 ggagagcagt cgttctcgtc gattccgctg gcgggcgcaa gagcatatta tccgctttcc 7080 tcagctcaga agcggctgtt tatcctgaat cagctggaag gggccgatca gagctacaac 7140 atgccgggcg tgctgctgct ggaaggatcg attgaccgga gcctgctgga gaaggctttc 7200 cgcggactga tcgcacggca cgaaacgctg cgaaccggct ttgagatcgt acaaggcgaa 7260 gctgtacagc gcatttacga gagcgtcgac tttgccgtcg ggtaccgtca tgtgagcgag 7320 gaagaagcgc ccgaagtcgt gcaggccttc atccggcctt tcgacttggc gaagcctccg 7380 ctgctgcggg cggagctcgt agagctggca gccgaacgtt atttgctgat gttcgacatg 7440 caccatatcg tctccgacgg ggtttcgatg gacgtgttag tcgaggaact cgttcgtctg 7500 tacggcggcg agtcattaga gcctttgcgc attcaataca aggactatgc ggtatggcag 7560 cagtcggacg agcaaaaagt gcagttgaaa cgcgaggaag cttactggct ggaccgttac 7620 cggggcgagc tgccggttct ggaaatgccg acggactatc cgcgtcctgc cgtgcagagc 7680 tttgagggac aaacgctgac gtccttcgtg gacgaggcaa cgaacgaagg cttgaagcag 7740 ctggccgctc aaagaggaac gacgctgtat atggtgctgc ttgcggcata taccgtgctt 7800 ttgcataaat acacaggtca ggacgatttg atcgtcggaa cgtcgattgc gggtagaacg 7860 cacggagaaa cgcagccttt gatcggaatg ttcgtcaata cgctggcgct ccgcaattat 7920 ccggcttcgg agaagacctt cctgtcgtat ctggaagaag tgaaagaaac gaccttaggc 7980 gcttacgagc atcagaatta tccgttcgaa gagctcgtcg ataaagtgca ggtcagccgg 8040 gatttgagcc gcaacccgct gtttgacacg atgttctccc tgcaaaactt ggaggataaa 8100 gagtttgagc tggaagggct gaaattgtcc ccgtacccta gcgaatacgg cacggccaag 8160 ttcgacctga gtgtggatgt tacggaagaa aacggcggcc tggagtgcag ctttgaattc 8220 gcaacggctc tttataaaga aagcacgatc cggcggctgt cgactcattt cggacatttg 8280 ctcgtaacga tcgtaagccg tccggatgcg aagatcgccg agctgaactt gctgacggca 8340 gaggaaaaag aacaaattct cggcgcgttc aacccggcgc agccggaagc ggctcctgcg 8400 tcggcgttcc accggctgtt cgaggaacag gcggagcgca cgccggaagc ggaggccgtc 8460 gtgtacgaga acgaccggct tacgtatgcg gagctgaacg agcgggcgaa ccgcttggcg 8520 gctacgctgc gtgcaagcgg catcggccgg gaggcgatcg tcggcattct cgccgagcgt 8580 tcggtggact tgctggtggc cgtgctggcc gtctggaaag tgggcggggc gtatgtgccg 8640 ctcgacccgg attacccggc ggagcgcgtg cggttcatgc tcgaagacag cggagcgaag 8700 gtgctgctga cgcaaacgcc gctgcgagag cgtgccgaag catggctcgg cgaagaggag 8760 ctggcgctgg cggcggtgct gtacctcgac gacgaagcgt cgtacagcga cgagcgggcg 8820 aatgcgtcgg ttgtctccgg caagctgacg gatgctgtag atgacggcga tgagagccat 8880 cagaatgttg gcaccgacag cttccatgaa gcccgtccgg aggatctggc gtacgtgatc 8940 tatacgtcgg gaacgacggg caagccgaag ggcgtgatga tcgagcaccg cagcctggtg 9000 aatacggcgg cgggctaccg gcgggaatac cggttggatc agttcgcggt gcggctgctg 9060 cagctcgcca gcttctcgtt cgacgtgttc gtgggagata tcgcccggac gctgtacaac 9120 ggaggcacga tggtgattgt gccgaaggac gaccggatcg atccgtctcg tctgcaccac 9180 tggattgagc gggagcgggt caccatcttc gaatcgacgc cggcgctgat cgtgccgttc 9240 ctagagtacg tgtacgagca gcaactggat atgagccggt tggagctgtt gatcacgagc 9300 tcggacagct gcagcgtggc ggattaccgg accttgcagg aacgcttcgg ctcgttgttc 9360 cggatcatca acgcatacgg cgtgacggaa gcggcgatcg actcaagctt ctacgacgag 9420 gagctggcga agctgccgca gacaggcaat gtgccgatcg gcaaagcgtg gctgaatgcg 9480 aaattctaca tcgtggatgc gcatctgaac ccggtgccgg tcggggtgct gggcgagctg 9540 gtcatcggcg gagtcggagt ggcgcgcggg tacttgaacc gtccggagct gacagaagag 9600 aagttcgtag acagcccgtt cgccgagggc gagcggctgt accgcacggg agacttggcg 9660 cggtggatgg aagacggcaa cgtggacttc atcggccgga tcgacaacca ggcgaaaatc 9720 cggggctacc ggatcgagac gggcgagatc gagtcgcagc tgctgcgggt ggaaggcgtg 9780 cgcgaagcgg tggtgctggt tcgaagtgac gcgaacgggc agaaggcgct atgcgcgtat 9840 tacacgccgg ataccggagc ggaactggcg gtgaacgatc tgcgcggcgc gctggcgcag 9900 gagctgccgg gctacatgat cccgtcgtac ttcgtggagc tggagcgcct gcctctgacg 9960 ccgaacggaa agattgaccg gaaggcgctg ccagcgccgg aaggggaagc gggaagcgga 10020 acggagtacg tcgcaccgcg caatgagctg gaaacgaagc tggcggcgat ttggcaggag 10080 gtgctggggc ttgcgaagga gattggcgtt cacgacaact tcttcgacat cggcggccac 10140 tccctgcggg cgacgacgct ggtcagcaag attcataaag agttgaacgt ggatctgccg 10200 ctgcgcgacg tgttccgcca ttccacgatc gagagcatgg cggccgccat ttcccggctg 10260 gatgagcaaa cattcgttgc cattccggtg gcggatgacc gagaggtgta cccgcaatct 10320 tttgctcaaa aacgtctctt tatcctgaat caactggaag gcgcggagct tagctacaac 10380 atgccggaag cgatgctgct ggagggtgct ttagaccggg caaggttcga agaagcgttc 10440 cgtaagctcg tggcgcggca tgaaacactg cgcaccgggt tcgaaatggt ggatggcgaa 10500 gcatcgcagc ggatttacca ggacgtgaat tttgccgtgg agttctatcg agcggatgag 10560 caagaggccg aagagacggt tcgccgtttc gtccgtccgt ttgacttggc gaagcctccg 10620 ctgctgaggg taggccttgt cgagctggct ccggaacgcc atattctaat gtacgacatg 10680 catcatatta tttccgacgg cgtctcgatg gaaatctttg ttgaagaatt cgtccgcttg 10740 tacggcggcg agcaattgga gcctctgcgc attcagtaca aagactacac cgtttggcag 10800 cattcgcagg agcagaagga acggcttcag cgtcaggagg cgtactggct ggacatgttc 10860 caaggcgagc ttccggtgct ggaaatgccg accgactatc cgcgtccggc cgtgcagagc 10920 tacgaaggcc aaacgctgga gtttttcttt gacgcttcga aaaccgacgg cctgaagcag 10980 ctggcctcgg aaacgggcac gacgctgttt atggtgctgc ttgcggcgta taacgtcctt 11040 ctgcataaat attcaggtca ggaagacgtg atcgtcggta cgccgattgc cggaaggaat 11100 catggagatg tgcagccgtt gatcgggatt ttcttaaaca cgctggctat ccgcagttat 11160 ccggcttcgg agaagacatt cctgtcatac ctgaacgaag tcaaagaaac gaccctccac 11220 gccttcgagc atcaaaacta tccgttcgaa gaattggtgg acaaggtgca agtcacccgt 11280 gatttaagcc gcaatccgct cttcgacacg ctgtttacga tgcagaatac ggagaacgag 11340 gaatttgagc tggaagggct tcgcctgatt ccttatccga gcgcactgga taccgcgaag 11400 tttgatatca gcttggatgt gggcgaggag aacggcggct tggattacag cttcgaatat 11460 gcgacggctc tctacaaaag ggagacgatc gaacggctgg caaaacatta cgagcagctg 11520 ctcgtaacga tcgtaagccg tccggatgcg aagatcgccg agctgaactt gctgacggca 11580 gaggaaaaag aacaaattct cggcgcgttc aacccggcgc agccggaagc ggctcctgcg 11640 gccgcgttcc accggctgtt cgaggaacag gcggagcgca cgccggaagc ggaggccgtc 11700 gtgtacgaga acgaccggct gacgtatgcg gagctgaacg agcgggcgaa ccgcttggcg 11760 gctacgctgc gtgcaagcgg catcggccgg gagacgatcg tcggcattct cgccgagcgt 11820 tcggtggact tgctggtggc cgtgctggcc gtctggaaag cgggcggggc gtatgtgccg 11880 ctcgacccgg attacccggc ggagcgcgtg cggttcatgc tcgaagacag cggagcgaag 11940 gtgctgctga cgcaaacgcc gctgcgagag cgtgccgaag cctggctcgg cgaagaggag 12000 ctggcactgg cggcggtgct gtacctggac gacgaagcgt cgtacagcga ggagcgagcg 12060 aatgcgtcgg ttgtctccgg caagctgacg gatgctgtgg acgacggcga tgagagccat 12120 cagaatgttg gcaccgacag cttccatgaa gcccgtccgg aggatctggc gtacgtgatc 12180 tatacgtcgg gaacgacggg caagccgaag ggcgtgatga tcgagcaccg cagcctggtg 12240 aacacggcgg cgggctaccg gcgggaatac cggttggatc agttcccggt gcggctgctg 12300 cagctcgcaa gcttctcgtt cgacgtgttc gtgggagata tcgcccggac gctgtataac 12360 ggaggcacga tggtgattgt gccgaaggac gaccggatcg atccgtctcg tctgcaccac 12420 tggattgagc gggagcgggt caccatcttc gaatcgacgc cggcgctgat cgtgccgttc 12480 ctggattacg tgcacgagca gcaactggac atgagctgga tggagctgtt gatcacgagc 12540 tcggacagct gcagcgtggc ggattaccgg accttgcagg aacgcttcgg ctcgttgttc 12600 cggatcatca acgcatacgg cgtgacggaa gcggcgatcg actcaagctt ctacgacgag 12660 ccgctgacga agctaccgca gacaggcaat gtgccgatcg gcaaagcgtg gctgaatgcg 12720 aaattctaca tcgtggatgc gcatctgaac ccggtgccgg tcggggtgct gggcgagctg 12780 gtcatcggcg gagtcggagt ggcgcgcggg tacttgaacc gtccggagct gacggaagag 12840 aagttcgtag acagcccgtt cgccgcgggc gaacggctgt accgcacagg agacttggcg 12900 cggtggatgg aggacggcaa cgtggacttc atcggccgga tcgacaacca gacgaaaatc 12960 cgggggtacc ggatcgagac gggcgagatc gagtcgcagc tgctgcgggt ggaaggcgtg 13020 cgcgaagcgg tggtgctggt tcgaagtgac gcgaacgggc agaaggcgct atgcgcgtat 13080 tacacgccgg ataccggagc ggagctggcg gtgaacgatt tgcgcagcgc gctggcgcag 13140 gagctgccgg gctacatgat cccgtcgcac ttcgtggaga cggaacgcct gcctctgacg 13200 ccgaacggaa agattgaccg gaaggcgctg ccagcgccgg aaggggaagc gggaagcgga 13260 acggagtacg tcgcaccgcg caatgagctg gaaacgaagc tggcggcgat ttggcaggag 13320 gtgctggggc ttgcgaagga gattggcgtt cacgacaact tcttcgacat cggcggtcac 13380 tccctgcgag cgacgacgct ggcgggcaag gtatttaagg aattaaacgt caacctgccg 13440 ctgcgcgacg tattccgtca ctcgacgatt gcggcgatgg ccgaggcgat cgcccggatg 13500 gaacggcggg agcatgaggc cattcctcaa gcggaggaga gagagtacta ccctctgtcc 13560 tccgcgcaga aacggctgtt cattcagcac acgctggatg gagcggatca gctttacaac 13620 atgccggaac tggtgcaggt ggaaggcgag tttgatttag accggttgga agccgccttg 13680 cggaaattga taacacggca tgaatcgctg cgcaccggtt ttgaaatcgt gaaggccgaa 13740 gcggttcagc ggatttaccc gcaggtcgat tttgctatcg agcatcatca agcggataaa 13800 gaggatgcgg ctcaaatcga gcagatcgtc cgcagcttcg ttcgtccgtt tgatctcggc 13860 aagccgccgc tgctgcgcgc cggggtcatc gagctggagc cgaacctgca tattctcctt 13920 ttcgacatgc accatatggt gtccgacggc gtatcgatgg cgattgtgat cgatgagttc 13980 tcgagtttct acgccgggga agaactgccg ccactgcgca ttcaatacaa ggattatgcc 14040 gtttggcagc agtcgaaggc ccaccgagag cggatcgggc ggcaggaagc gtactggctg 14100 caaaccttcg aaggcgagct gccgacggcg gacctgccga tggactacga acggtccgcg 14160 gctcgcagct acgaaggcgc gcatctggag ttcgacgtcg aagcttctct ctctgcgcgg 14220 ctgcgcgaat tggcggccga gcgtgaaagc acgctgttca tggtgctgct tgcggcttat 14280 accgtgctgc tgtccaagta cagcgggcag gaggacttgg tcgtgggcac cccggtggcg 14340 ggaagaacga acgccgattt ggaaccggtc atcgggatgt ttgtcaatac gctggcgatc 14400 cgcaatcgtc cgtcgggcga caaaacgttc ttgtcctacc tggaagaagt gaaggaaacg 14460 gctttgggtg ctttcgagaa ccaggattat ccattcgagg agctcgtgga gcgtttgaat 14520 gtgaagcggg agccgggccg cttcccgctg ttcgatgccg ttttcgactt gcaaaatatc 14580 gaagaacgag acgccgagct ggaaggggtc agcctgaaga cttacgagct tgaccatttg 14640 gaagaagcga agttcgatct gacgctgttt atgtatgaaa acaacggggc gctgagcggg 14700 ggcttcttct acgccaccaa gcttttcaaa gaagcgatga tccgcacctt gaccgaggat 14760 tacctgcggg tactgtctca aattgcggaa aatccacaac tcgagctaag ccggattgaa 14820 tgtcataaac cggcggcagg cgcaaagagt gccgtcgata cgatcgaatt cgcgttctaa 14880 14880 <210> 8 <211> 3309 <212> DNA <213> Paenibacillus polymyxa F4 <400> 8 atgaaatctt tgtttgaaaa ggaagaacgg tactggagcg gcaagtttga cgccgatgac 60 agcctgagct tccttcccta cagtcaatcc tccaaattat ccgccgacgg ggaagctgcg 120 gccgagccgg gcttgcttca ccgtactctg ccgagcgaac tctcggagag aatcatttgc 180 ctcgccaacg gttcggattt ggctttgtac atgattgttt tggcaggagt aaaaagcctg 240 ctgttcaagt ataccgggcg ggaccaagtg ctggtcggca tgccttctta tagcgcagac 300 cccgacggga ctccgccgcc gcatgacatc ttggtgatca agacgtccgt aagccgccag 360 actacgctga aaacgctgct cgggggcatc aaagcctcca tcggcgaggc gctggagcat 420 cagcacctgc cttttcggaa aatggtggag ccgctccatc tggactatac gggggacggc 480 ctcccggtcg tcaacaccgt cgtatccttc gccccgattc atccggaacc gctgggtaac 540 cgggtggcgg ccgatacggt ttttcgcttc gaccgccaaa accactccat cgagctggaa 600 ataagctttg acgggcagcg ttacgagcgg gcatttgtgg aacaggcggc cgaccatctt 660 gttaggctgc tgtccgtgct tttatttcag ccggatctgg agcttggaca agccgatgtg 720 ctgtccccag acgagaggga gacgctgctg aagcgattta atgacaccga aaccgagttc 780 gagcggggga aaacgattta cggcttgttc gaagaacagg cggagcttta cccggacaac 840 gtggccgccg tcatgaacga gcggcagctg acctaccgcg agctgaacga gcgatccaac 900 cgccttgcgc ggaagctgcg agagacggga gtagaagcgg accagctggt agcgattctg 960 gccgaacgct cgctcgatat ggttgtcggc attctggcga ttctcaaagc gggcggagca 1020 tacgtgcctg tcgatcccga ctacccggag gagcgcatcc gcttcatgat cgaggattcg 1080 ggcgcgccgt tattgctgat tcaaaagcat ctgcacgaga agaccgactt cgcaggaacg 1140 cgccttgaat tggacgattt cgtgtggggc gacagagggg cggactccgc cgatgtgcgg 1200 gacgcttcga acctggagcc gatttccggg ccgggcaacc tggcttatgt catctacacg 1260 tcgggaacga ccggcagacc gaaaggaacg ctgatcgagc ataagaacgt cgtgcgcctc 1320 ctgttcaacg acaagaacct gttcgacttc gggccgtccg acacgtggac gctgttccac 1380 tcgttctgct tcgatttctc cgtctgggaa atgtacggag cgctgctgta cggaggcaag 1440 ctggtcatcg taccgccgct cacggcgaaa aatccggccg atttcctggc gctgctgggc 1500 cgcgaacagg tcacgatttt gaaccagacg ccaacgtact tctaccagct gctgcgtaag 1560 gtcttggcgg accatccgta cgatctgcgg attcgcaacg tcatcttcgg gggcgaagcg 1620 ctgagtccgc tgctgctcaa gggattcaag acgaagtacc cggagacgaa gctgatcaat 1680 atgtacggca ttaccgagac gacggttcac gttacgtata aggaaataac gtgggtcgaa 1740 atggaggcgg cgaagagcaa tatcggcaag ccgatcccga cgctgagcgt gtacgtcctt 1800 gatgaaaacc gctcccctgt gccgatcggt gtagcgggcg aaatgtacgt ggccggggaa 1860 ggccttgcga gaggatacct gaaccgtccg gatctgacgg cggagaagtt cgtcgattcc 1920 ccgtttgcgg agggggagaa actgtaccgc tcgggcgact tggcggcttg gctgccggac 1980 ggcaacatcg aatacctggg ccggatcgat catcaggtga aaatccgtgg gtaccggatc 2040 gagctggacg aaatcgagac gcagctgctg aagatcgccg ccgtgcaaga agccaaggtg 2100 ctcgaccgcg acgacgcgaa cggccataag cagcttgtcg cttattacgt cgcggaaacg 2160 aggctggcgg cgcatgaact caaggaggag ctcgccaagc agcttccggg gtatatgatt 2220 ccttcgcacc tcgtgcagct ttcgcggatg ccgctgaccc cgaacgggaa aatcgaccgc 2280 aaagcgctgc ccgcgccgga ggaagccgcg gccggaggag cggaatatgt cgcgccgaga 2340 acgctgctcg aaatgaagat cgcccgcgtc tggcaggata cgcttggcgt tccgcaggtc 2400 ggcgtaaagg ataacttttt tgagttgggt ggcaattcgt taagtctgat gaggctcgtt 2460 caagccgttt acgatgaaac ggacatcgag ataccgctga accgccaatt ccacaatcta 2520 acagttgaag ccatggcttt cggcgagggg gatctgggcc tggataaagg gggagactcc 2580 ttcattaagc tgaataaagc aggagatctg aacgtgttct gcttccctcc gggcagcggc 2640 ttcggcatcg gttaccgaga gctcgcaagc aggctcgacg gccggttcgt gctctacggc 2700 attgatttta tcgacgatgc cgtcgattac gaggccatgc tgaaccgtta tgtggacgag 2760 atcgtccgca tccagccgga aggaccttac gtgctgctcg gctactgctt cggaggcaac 2820 ctgacgttcg aggtagccaa aacgatggag aaaagagggt attccgtaac ggacgtgctc 2880 atgttggact cgtggattaa ggacacgctg acgccttccg aaacgtcgga gaaagagctt 2940 gaagaaacgc ttgccgattt cgacgaagaa gagaaggaat taatgagcaa cccgctcgtg 3000 cgggagcgag ttcatcggaa ggtcaaagcg accttggcgt acgaagcgca gcttattaat 3060 tccggcacga tcccggccag gatttacgaa ctgattgcga aggacagcga agcgttccgc 3120 ctggagcacc aattgccttc ctggcggggg gcaacgacgc aagcttacgc cgattaccgg 3180 ctggagggcg cgcacgagga attgctggaa ctcgcgcgcg tggaagaaac ggccgtcgtc 3240 atccgggaca tcttagagca agtcaagcgg cggatcgaag cggaggccgg ggtactgcat 3300 ggaagctga 3309 <210> 9 <211> 18849 <212> DNA <213> Paenibacillus polymyxa F4 <400> 9 gtgagagaga ataccaacga gcaatatgga ttaacgcaag cccagcgccg aatatggttc 60 atggaaatta tgaatccggg aacgtccatc acgatgcttt ccgcgaccta ccagattacg 120 ggcgagatca acacacagct tctggagcaa gcggcggcag agatcgtcaa aacctatgac 180 gttttccgaa tccgcattag cggggatttg caaaatccaa cgcagtggtt cgaagaaccg 240 gagaatgtcc aagctaggat aagccgcctc gaaataggca caaccgaaca attctatgct 300 tgggtgaaag aagtaagcga aaaaccggcc agcgtgttcg acgaacacct ctaccaattt 360 acgattatcc attttgcgaa cggccaagta tggctcaatt tgacggtaaa tcatattatc 420 gccgacggct tgtccgtcaa tgctttgctg catgcggtga tggaaaaata cctggaactg 480 cgcaaaggca tctccagcag ttaccaggcc ccttcctatc tggattatat ttctgcggag 540 cgtgaatatg agcaatcgca gcgttatcaa aaaggcaagg aatactggct gacgaagtac 600 aacactttgc ctgaaacgac cggcattaaa tcgtatccgc cattctcgat cggcagcgaa 660 tccaataaac tggccatcac tttggacggt tcccggtatg aacgcattct ggccttcagc 720 gaacaatatc aggtcagctt atatacgtta tttctgtccg ccatgtacgc cttattgtac 780 aagctgaccg acagcaccga tgttccggtc ggcacggttt tcgccaatcg caccagcaag 840 aaggaaaaag aaacgatcgg catgttcgtc agcaccgtgg ctacgcggat ccgtctgaat 900 ccagacaggc acgtgctttc cttgatccaa acggtttcca aggaaaatac ggcggatctg 960 cggtatcaga aataccctta taaccaattg atccaggatt tacgcgaaca acacggccgc 1020 aacgatcttt cggggctgtt ccgcacgtct ctggaatatc tgcctttgaa aatcgtggag 1080 tacgaagaaa tcaaggtacg cctggaggct cacttcgcta ggcacgagat ggacgatttg 1140 ctgctgcgct tcgaccatat gctgaatgaa ggccatgtaa ttctccatgc ttcctatcgt 1200 accggcctgt tcgagacggc cgagattgat cggattatgg aacagtatgt aaccgttctg 1260 gaccagtttc ttcagactcc cgaactgccg gtacgcgaga tttctctgct gagcgatgag 1320 gagagacacc gcattctcaa cgtttttaac ccgccggtgg cagggctgag cgagggagaa 1380 gcgtttcatc ggtatgttga aaagtttgcc ctggaaattc ctgatcatcc ggcagtcgtc 1440 tacatggaca aacagctgac ctacggcgaa ttgaacgaac gcgccgagcg gctggcttct 1500 ctccttcgcg aacagggcgt gggaagggag acgattacgg ggatctgggc ggagcgttcg 1560 gtggaactgc tggtcggggt gctcgccgtt tggaaagccg gcggagccta tgtaccgctg 1620 gaccccgatt atccggcgga gcggattgag tacatgctca gcgatagcga agcaacggtg 1680 ctgcttacgc agcgtcatct gttggagcgg gccggagttt ggttggccga tgaccggctg 1740 aagcttcaag ctgtctatgc catggacgat gaacagattt ataacgggga tgccttagcc 1800 gtggaatttg aatctgccgg cagcgccccg caagacttgg cttatgtgat ttacacctcg 1860 ggtacgacgg gacgcccgaa aggcgtcatg atcgagcacg gtagtctcgt gaatacggcg 1920 gatgcgtacc gtcgcgagta ccggttggat cagtttccgg tgcggctgct gcagctggcc 1980 agcttctcgt ttgacgtgtt cgtcggagac atcgcgcgga cgctgtataa cggaggcacg 2040 atggtgattg tgccgaagga tgaccggatt gatccgaacc tcttatacgg ctggattcgg 2100 gaccaaaata ttacggtatt cgaatcgacg cctgcgctca tcctgccgtt catgcagcat 2160 atttatgaag aagggctgga cgtcagctcc atacagttgc tgattaccag ctcggatgct 2220 tgcagtgtca ccgattaccg attgctgctg gaaagattcg gcggacaatt ccgcatcatt 2280 aacagctatg gcgttaccga agcggccatt gacagcagct tttacggtga gccgctggat 2340 aagctgccgc cgtcgggtca tgtgccgatc ggcaaagctt ggctgaacgc ccggttttac 2400 attgtcgatg ccgcgttaaa gccagttcct gtaggggttc cgggcgagct tgtcataggc 2460 ggcgccgggg tggcgcgcgg gtactggaac cgtctggacc taaccgccga gaagtttgtg 2520 gacagcccgt ttgtgccggg cgaacgtgtg taccggacag gcgatttggc ccgctggttg 2580 gaagacggca acgtcgactt catcggccgg atcgactatc aggtgaaaat tcgcgggttc 2640 cgtatcgaac ttggcgaaat tgaaacggcc ctgctgcgtt tcccgggcgt caagcaggct 2700 gtggtgaccg accgcacgga tgagcagggg caaaagtatt tgtgcggcta cgtggcggcg 2760 gatgcttcct tgcagctgag cgatctgctg tcccaattga agcaagagct gccggcccat 2820 atggtcccgg cccggctggt gtctcttgat aagcttccgc tcacgccgaa cggcaaaatt 2880 gaccgtaaag cgctgcctga accgaccgga gaggtagaaa caggcggtga gtatgtggct 2940 cctcgcacaa cgctggaaac aagacttgct ctcatttggc agcaggtgct gggtattgcg 3000 cgagttggag tccaagacga tttctttgac ttgggtggtc attccttgcg ggcctccacg 3060 ctggtttcca agattcggaa agagctgcaa gtcgaggttc cgctgcggga agttttccgc 3120 tacaccacga tcgaacagct ggcccaaaga atcggcggtt taaggcagca ggagacgtat 3180 gagattacaa aggcggctga ggccgagtac tatccggttt catccgagca aaagcgtctg 3240 tacgtcctgc gccagcttga cggggccgag cgcagctaca atatgtcggc ggcgcttctt 3300 ctcgaaggca agctggaccg cacgcgcgtc gagcacgcgt tccgggcgct gattcagcgc 3360 catgagacgc tgcgtaccgg gatcgagcag gttcaaggcg agcttgtcca gcgcatctat 3420 gacgaggtgg agtttgctgt agattatttc caagcgagcg agtgggaagt ggaccaagtg 3480 gtggaagcat actatcaccc gtttgatctg accaagccgc cacttctccg catcggcctg 3540 atcgaagtcg acgaggatcg ccacattctg ctgttcgata tgcaccatat cgtctcggac 3600 ggcatttcga cagcgctgct cttcgacgag ttcagccgcc tgtatcgggg cgaggagctg 3660 gccccgctgc gcattcaata caaagattat gccgtttggc agcattccga agcttacggg 3720 cagatgctcc agccgcagaa ggagtattgg ctgaaacagc tgtcgggcga gctgccggtc 3780 ttggagctgc cgacggactt cccgcggcct gcggtgcaaa gctttgacgg ccgaaccgtg 3840 aagttttata tcgagaaaga tcggacggag aagctgaaag agctggcggc acggacaggg 3900 acgaccctgt acatggtact gctgtcggct tacaccatcc ttatgcataa atattcgggt 3960 caggaagatc tgatcgtagg aacgccgatt gccggaagaa cgcaggaaga agtgcagccg 4020 atcgtaggga tgtttatcaa cacgctggcc attcgcagcc gtccggagcg ttccaagccg 4080 tacctttcgt acctggaaga aatcaaggac atcacgctcg gggctttcga acaccaaaat 4140 tatttgttcg aagacttggt agaaagtctt cacattccgc gcgcgaccgg ccggaatccg 4200 ctctttgaca cgttcttctc cctacaaaat acggagaacg agcaaattgt catcgagggg 4260 ctggagcaat cgttttatcc actggaaaac caaacatcca agttcgagct gctcctggat 4320 atttcggagc tggacggtca gctcgaatgc cggttggagt acgctacggc tttgtataaa 4380 caagagaccg cggagcggtt cgccagacat tatgacaagc tgctcgaaac catcgcagca 4440 gcgccggacg gggatattgc ctcgctggaa atgctcacgg aggaggaaat ccgcgagttg 4500 gtgcgcggtt tcaacgattc ggaggcggac tacccgcggc agcagacgat tcacggcttg 4560 tttgaagagc aggcggagct ttatccggac aacgtggccg ccgtcatgaa cgagcggcag 4620 ctgacctacc gcgagctgaa cgagcgatcc aaccgccttg cgcggaagct gcgagagacg 4680 ggagtagaag cggaccagct ggtagcgatt ctggccgaac gctcgctcga tatggttgtc 4740 ggcattctgg cgattctcaa agcgggcgga gcatacgtgc ctgtcgatcc cgactacccg 4800 gaggagcgca tccgcttcat gatcgaggat tcgggcgcgc cgttattgct gattcaaaag 4860 catctgcacg agaagaccga cttcgcagga acgcgcctcg aattggacga tttcgttggg 4920 ggcgacagag gggcgaactc cgccgatgtg cgggacgctt cgaacctgga gccgatttcc 4980 gggccgggca acctggctta tgtcatctac acgtcgggaa cgactggcag accgaaagga 5040 acgctgatcg agcataagaa cgtcgtgcgc ctcctgttca acgacaagaa cctgttcgac 5100 ttcgggccgt ccgacacgtg gacgctgttc cactcgttct gcttcgattt ctccgtctgg 5160 gaaatgtacg gagcgctgct gtacggaggc aagctggtca tcgtaccgcc gctcacggcg 5220 aaaagtccag ccgatttcct ggcgctgctg ggccgcgaac aggttaccat tttgaaccag 5280 acaccaacgt acttctacca gctgctgcgt gaggtcttga cggatcatcc atacgatttg 5340 cggattcgca atgtgatctt tgggggcgaa gcgctgagtc cgctgctgct caagggcttc 5400 aagacgaagt acccggagac gaagctgatc aatatgtacg gcattaccga gacgacggtt 5460 cacgttacgt ataaggaaat aacgtgggtc gaaatggagg cggcgaagag caatatcggc 5520 aagccgatcc cgacgctgag cgtgtacgtc cttgatgaaa accgccgccc tgtgccgatc 5580 ggcgtagcgg gcgaaatgta cgtggccggg gaaggccttg cgagaggata cctgaaccgc 5640 ccggatctga cggcggagaa gttcgtcgat tccccgtttg cggaagggga gaaactgtac 5700 cgctcgggcg acttggcggc ttggctgccg gacggcaaca tcgaatacct gggccgaatc 5760 gatcaccagg tcaaaatccg cgggtaccgg atcgagctgg acgaaatcga gacgcagctg 5820 ctaaacgtcg agggcgtgga agaagcggtg gtgcttgccc gtcaggacgg cgggggcgag 5880 aaggcgcttg tcgcctactt tgtggcggac cggacactga cggtcagcga aatgagaacc 5940 tcgctggccc agggaatgcc ggggtacatg atcccgtcgt acttcttgca gcttgagcgc 6000 atgccgctga cgtccaacgg taaagtggac cgcaaagcgc tgccggagcc gcaaggcggc 6060 ttgcaaacgg gcgtcgaata cgtagcgccg cgtaaccgga cggagtccca gcttgtgaag 6120 atctgggagg aagtgctggg ttactccggc attggagtcc tggacaattt cttcgagctc 6180 ggaggccact ccttgcgggc gacgaacctt gtcagcaaga ttcggaagga aatgaacgtc 6240 gaacttccac tgcgcgatgt gttccgctat acgacggtag agtcgatggc cggggctatt 6300 gccagcttgg aagaaacaca gcacagctcg attccgaaag cggaagagaa agcgtactat 6360 ccggtttcct ccgcgcaaaa aaggctgtac gtcctgcacc agctggatag ctcggagctg 6420 aattacaacc tcccaagcgc cttgcaattg gaaggggctt tgaacgaggc caaagtggaa 6480 aaggcgctga ctactttggt ggcccggcac gatatgctgc gcaccggttt tgaaatcgtg 6540 aatggggagc cggttcagcg tattcatccg ctcgtagctt tcaaggtcga gaagcttcaa 6600 gcaagcgaag atcaggttgc ggccattctt gaaggcttca ttcagccttt tgacttgacc 6660 cagccgcctt tgctgcgcgc cctactgatc gaactggaga aagagaaatt cctgctcgcg 6720 ctggatattc atcatattgg ttccgacggc ctttccatgg acgtgctgct gcgcgaattc 6780 gtgcggcttt acaatgggga agaattgccg gagctgcgga ttcaatacaa ggattacgcc 6840 gtttggcagc aatccgagga acagcgccag cgcatcaaac ggcaggagga atactggcgc 6900 ggggtattca gctccgagct tccggttctt gagctgcctc tcgacttctc ccgcccggcc 6960 gtccagcagt ttgacggtca aacgctcacg tttacgctgg atgcggagaa aagcgaagct 7020 ctcaaacggc ttgccggcga ttcgggggcg acgctgtaca tgcttctgct ggctgcttac 7080 tccgtattgc ttcataaata cgcgggacag gaagatatag tggtcggcac tccgattgcc 7140 gcccgttctc acgccgacct gcagccgatt atcggcatgt tcgtcaatac gctggccctt 7200 cgcttgggtc cggtggcgga gcggacgttc ctggattact tgcaggaagt gaaggaaacg 7260 acgcttggag cctacgagca ccaggactat ccgttcgagg agctggtgga agctcttcag 7320 gtgagccggg atttaagccg gaatcctctg tttgacacca tgttttcttt gcaaaagcac 7380 gaaagcttgg atttaaccct ggaaggcttg caatggtcgc tgttcgacat cgaggaaaag 7440 acggcaaagt ttgatcttag ctttgatatc gtggaagccg ataacgagct gatttgcaag 7500 atcgagtacg ctacctcgtt gtttagacag gaaacgatgg tacggctggc gggtcattac 7560 gagcagcttt tggcgtcgat cctggctcag ccgggtgcgc ggatttcgga tttggatatc 7620 ttgacggaca gcgaaaagca tgatttgctg gtcgggtttg acgtgtcgtc ttcggctctt 7680 gcgaagcaac ccgccgcaga gggtacaggt ttggaagcgg atgagtcgtg gagagagaga 7740 acgttccacg agctgttcga ggagcaggcg gagcgcactc cgggagcgct ggcggttgtc 7800 tacgaagaca gcaagctgac gtatgcggag ctgaacgcca aagcgaatcg tctggcgtat 7860 gcactgcggg cgcgcggggt gaagccggag caggtggtcg gcattctggc cggccgttcg 7920 gcggagctgt tgatcggggt gctcgccgta tggaaagcgg gcggcgctta tgtgccgctc 7980 gacccggact atccggcgga gcggatcgag tatatgctcg cggacagcgg ggcgtcggtg 8040 ctgctcacgc agacccgcct gctggagcag gcggaagctt ggcgcagcga cggagctcta 8100 gtgctgcaaa cggtgcttgc gcttgacgac gccgcgacgt acagcctcgg agcggcggaa 8160 gcggctgtgg gcgaagcagg cgcagaggcg gaggctttgg cgcaagcggc tgctgccgaa 8220 tcgtccgcca cggcagaagc cgagaagaac gtactagcgg cggatctcgc atcgaatccg 8280 gcgaatgtga acaagccgcg cgatttggct tacgtcatct acacctccgg tacgacgggc 8340 cgtccgaagg gcgtggcggt ggaaaaccgc agcctggtga acacggcggc gggctaccgg 8400 cgggaatacc ggttggatca gttcccggtg cggctgctgc agctcgcaag cttctcgttc 8460 gacgtgttcg tgggagatat cgcgcggacg ctgtacaacg gcggcaccat ggtcatcgtg 8520 ccgaaggacg accggattga tccaacccgc ctctacggct ggattcgcga ctacgccgtg 8580 acggtgttcg aatcgactcc ggcgctgatc gtgccgttca tggagcatgt gcatgccgag 8640 ggtctggacc tcagctcgat gcagctgctg atcacaagct cggatgcgtg cagcgtagcg 8700 gattaccgca ccttgcagga gcgcttcggc tcgcagttcc gtattattaa cagctacggc 8760 gtaacggaag cggcgattga ctccagcttc tatgacgagc cgctggagaa gctgccgaag 8820 acgggcagcg tgccgatcgg gaaagcgtgg ctgaacgcca agttttacat cgtggatgcg 8880 aatctgaagc cggtgccgat cggggtgttg ggcgagctgg ttatcggcgg agcgggtgtg 8940 gcccgaggtt acttgaaccg cccggatttg acggcggaga aattcgtaga cagcccgttc 9000 gccgctgggg agcggctgta ccggacgggc gacctggcgc gctggatgcc ggacggcaac 9060 gtggacttca tcggccggat cgacaaccag gtgaaaattc gcggctatcg gatcgagctt 9120 ggtgaaattg aagcggccat gaaaaatttt gccggcgtgc gtcaagcgct tgtcatcgac 9180 cggacggacg agcgggggca gaaatatttg tgcgggtatg tcgtagcgga ttccagcttc 9240 gatctggaag ggcttgtggc ccatctggac gccgcgctgc cttcccatat ggtgccttcg 9300 cgcatcatgc gcctggatca aatgccgctt acgccgaacg ggaagatcga ccgtaaagcg 9360 ctgcctgtac cggaaggaag cattcgtgcc gaggctgcat acacggcgcc tcgtactcct 9420 gccgagcaag cacttgcgtc ggtctggcag tcagtgctgg gcgtggatca ggtcggcacg 9480 atggacaatt tctttgcgct cggcggcgat tcgatcaagg ccttgcaggt atcgtcccgt 9540 cttttgcaaa cggggtacaa gctggtcatg aaagatttgt tccattaccc gacgatttcc 9600 gcccttagtt tgcagctgca aacggcggag agaacggcaa gccaggccga agtgacggga 9660 gaggtcatct tgaccccgat tcagcgttgg ttctttgaac aaaatccggc cgacgtgcat 9720 cacagcaacc aggcgttcat gcagttctcc aagcaaggct ttgacgaaga agctttacgc 9780 caagcggtgc gtcagcttgt cgtgcatcac gatgctctcc gtacggttta ccgccaaact 9840 gagaacggct ataccgcctg gaatcgcggc gccggggaga acgaagcgct gttcgatctg 9900 gaagttgtcg atttcaaggg agtcggcgac gtgaaagaag cggtagaggc taaggcgaat 9960 gacattcaag cgagcatcga tctggaaaac ggtccgctgg tgaagctcgg cttgttccgc 10020 tgcgacgacg gcgaccacct gctcatcgcg atccatcact tggtcgtaga cggcgtatcc 10080 tggcggattc tgcttgagga ttttgctgcc tgctatgagc aggcgctgca agggcagccg 10140 atccgtctgc cgctcaaaac ggattcattc caaacgtggg cgaaacagct cgctgattat 10200 gcgaacggtc cggcgatgga aagcgagaga gagtattggc agcatatcga gcaattgacc 10260 tatgagccgc ttccgaaaga ttttgaacaa ggcagatcca agctgaagga cagcgggctc 10320 gtgaccgttc gctggacggc ggaggaaacc gaacagctgc tgaagcaggc acactgtgct 10380 taccatacgg aaatgaacga tttgctgctt gccgcgcttg gcctcgcggt acaagcttgg 10440 agcggccggg aacgcgtgct ggtgaatctc gaaggccacg gccgggaaga tattttgccg 10500 aatgtggaca ttacgcgcac ggtaggctgg tttacaagcc aattccctgt cgttctggag 10560 ccgggtcacg cccaggcgct cggtcatcag gtaaaacagg ttaaagaaag cttgcgccgc 10620 attccgaaca aaggaatcgg ctacggcatc ctgcgttatt tgtcggcgcc gcgtgacggc 10680 gagcgcttcg ctttggagcc ggagatcagc tttaactatt tgggtcagtt cgaccaggat 10740 tacgaaagca gcggctcgag gccgtctccg ttcagcccgg gctccgattc aagcccggat 10800 gcagtgatgg attatgtcct cgatatcaac ggtatggtgt cggaaggagt gcaggaactc 10860 acgatccgtt atggggaaac ccagtataaa cgggaaacgg tagagcgcct gggcaccctg 10920 cttcattcga gcttgcgtga agtcatcagc cattgcgtat cgaaagagcg gccggagctt 10980 acgcctagcg acgtactgct tcaagatgtg acgttggagg aactggagcg gctggccgaa 11040 catacggcgg cgctcggcga actggagaat gtatacaccc tgactccgct gcaaaaaggg 11100 atgttgttcc acagcctgct ggatgccgat tcggaagctt acttcgaaca ggtgaccttc 11160 gatctgtacg gaagcctgaa tgtcgaagcc ttcacccaag gattggatac gctggtgcag 11220 cggaatgagg cgctgcgtac caactttatt accggctgga gagacgagcc gattcaagtg 11280 gtattccgcg agcggaagtg tgaagtgtac ttcgaagata ttcgctcggt aagcgatgaa 11340 gacccggaga agacgatagc cgatttcgtc agcgcggata aagcgaataa gttcgatttg 11400 gcccgaggct ctcttatgcg cgtgacagtt ttgcgcacgg gcgacgagtc ctaccatgtg 11460 atctggagtc accatcacat tttgatggac ggctggtgca tgtccttcat gatcaaggaa 11520 gtgttcgaca cctacttcgc tttccaggag aagcggacgc tggagcttcc tccggttacc 11580 tcgtactccc ggtatatcga atggctggaa gctcaagatg ccgcgaaagc ttcgcgttac 11640 tggtccgagt atttggcggg ttacgatcag cagaccaagc tgccccagga gaaaacgcag 11700 ctgaagcagg gcgcttttga agcggctgaa atcgatgtgg aactcagcaa ggaactgacc 11760 gggcaaatcg agcgggtggc gcgccagcag caggtgacgc tcaatacgtt catgcagacc 11820 gtatggggac tggttctgca ggtatacaac aacagcgagg atgtcgtatt cggctccgtc 11880 gtatccgggc gtccggcgga aattccgggc atcgaaagca tgatcggcct gtttattaac 11940 acgatcccgg tccgtattca aggcaaagcc gaggagacgg tagccgatat cttgagaaaa 12000 acccaggatc aagcgctggc atcgggagct tacgaaacgt tcccgctgtt cgaaattcag 12060 tcgctgagcg agcacaagcg cgacttgatc aaccatatca tggtctttga aaattatccg 12120 atggaagaac agattgagca ggtcgtcggc ggagacaaag aagcgctgaa aatcgctaat 12180 atccagtcgc cggagcaaac gaactacgac ctggacatta ccgtcattcc ggaagagcat 12240 attttgctgc ggtttacgta caatgcgctg acgttcagag aggaagacat caggctgatc 12300 cacggtcatt ttgcccgggt actggagaag gttgcggcta acccgagcat ccgcgtgaat 12360 cagttggagc ttttgacggc ggcggaaaaa gagcaaattc ttggcgcgtt taacccggcg 12420 cagccggaag cggctccggc ggccgcgttc caccggctgt tcgaggaaca ggcggagcgc 12480 acgccggaag cggcagctgt cgtgtacgag aatgaccggc tgacgtatga ggagctgaac 12540 gagcgggcga atcgcttggc ggccacgctg cgcgcaagcg gcatcggccg ggagacgatc 12600 gtcggcattc tcgccgagcg ttcggtggac ttgctggtgg ccgtgctggc cgtctggaaa 12660 gcgggcgggg cgtatgtgcc gctcgacccg gattatccgg cggagcgggt gcggttcatg 12720 ctcgaagaca gcggagcgaa ggtgctgctg acgcaaacgc cgctgcgaga gcgcgccgaa 12780 gcctggctcg gcgaagagga gctggcgctg gcggcggtgc tgtacctcga cgacgaaact 12840 tcgtacagcg aggagcggac gaatgccgac agcttccatg aagcccgtcc agaggacctg 12900 gcgtacgtga tctacacgtc gggaacgacg ggcaagccga agggcgtgat gatcgagcac 12960 cgcagcctgg tgaacacggc ggcgggctac cggcgggaat accggttgga tcagttcccg 13020 gtgcggctgc tgcagctcgc aagcttctcg ttcgacgtgt tcgtgggaga tatcgcgcgg 13080 acgctgtaca acggaggtac gatggtgatt gtgccgaagg acgaccggat cgatccgtct 13140 cgtctgcacc actggatgga gcgggagcgg gtcaccatct tcgaatcgac gccggcgctg 13200 atcgtgccgt tcctggagta cgtgcacgat cagcggctgg atatgagctg gatggagctg 13260 ttgatcacga gctcggacag ctgcagcgtg gcggattacc ggaccttgca ggaacgcttc 13320 ggctcgttgt tccggatcat caacgcatac ggcgtgacgg aagcggcgat cgactcaagc 13380 ttctatgacg aggagctggc gaagctgccg cagacaggca atgtgccgat cggcaaagcg 13440 tggctgaatg cgaaattcta catcgtggac gcgcatctga acccggtgcc ggtcggggtg 13500 ctgggcgagc tggttatcgg cggagtcggg gtagcgcgcg ggtacttgaa ccgtccggag 13560 ctgacagaag agaagttcgt agacagcccg ttcgccgcgg gcgagcggct gtaccgcacg 13620 ggagacttgg cgcggtggat ggaggacggg aacgtggact tcatcggccg gatcgacaac 13680 caggcgaaaa tccgggggta ccggattgag acgggcgaag tcgaagcgaa gctgctgagt 13740 gtaggtggcg tgaaggaagc ggtcgtcgtc gtcagggaag atcaagaagg tcagaaagct 13800 ttgtgcgctt attatacagc ggaagaaggc ttgacggcgg cggacctgaa gcgggcgatt 13860 gccaacgagc tgccggggta catgatcccg tcgtacttcg tggagctgga gcacctgcct 13920 ctgacgccga acgggaaaat cgaccggaag gcgctgccgg cgccggaagg gggagcaagc 13980 gcaggccgcg aatacgtggc gccgcgcacc gaacttgagg cgaagctggc cgccatttgg 14040 caggatgtgc ttgttcggga gaaggcggta ggcgtaacgg acaacttctt cgacctcggc 14100 ggacactccc tgcgggcgac gacgcttgtc agcaaaatgc ataaggagct aggcgttgaa 14160 tttccgcttc gcgacgtatt ccgctactcg acggttgagg aaatggccgc ggctatggag 14220 cggctggaga tcggctcgtt cacggctatt ccggctgcgg aacctagcga gtattatccg 14280 ctctcttccg ctcagaaacg tctctatatc ttgaaccagc tggaaggagc cgagctgagc 14340 tacaacatac cgggagctat gctgctcgaa ggggagctcg accggcagcg gtttgaagaa 14400 gcgttccgcg ggctcgtagc tcgtcatgaa acgctgcgta ccggctttga gatggtaaaa 14460 ggcgaagcgg ttcagcggat ttacgaagaa gccgccttcc aggtggaata tgtgcagatc 14520 agcgcggagc aggcggaaga aacggtgcgc caattcgttc gtccgtttga tctagcgaag 14580 ccgccacttc tgcgggtggg ccttgccgaa ctggcgccgg accggcacat tctaatgttc 14640 gatacgcatc atatcgtatc tgacggcgtt tcgattgacg tactgatcga agagttcgtc 14700 cgcttgtaca gcggggagca gttggagccg ctccgcattc agtacaaaga ttatgcggta 14760 tggcagcaat cggacgagca gaaagctcag cttgccaagc aggaagccta ctggctcgac 14820 atgttccgcg gagagctgcc ggttttggaa ttgccgatgg actatccgcg cccggctatg 14880 cagagctacg agggccgcac gctgcaattg tttatgcata gcgagaaaag cgagggtctg 14940 aaacggcttg cagccgagaa cggcgcaacg ctttacatgg ttctgcttgc tggttataca 15000 atattattgc ataaatatac agggcaagaa gacgtggtgg tcggtacgcc gattgcggga 15060 agaaatcaca gcgacgttca gccgctgatc gggatgttcg tcaataccct ggccatccgc 15120 agttatccgg ctgccggtaa gacgttcctt gactacttga aggaaatcaa ggagacgacg 15180 ctgggcgctt ttgaacatca gaattatccg tttgaggaac tggtggataa ggtgaacgtg 15240 gctcgcgatt taagccgcaa tccgctgttc gatacgatgt ttgctttgca gaatacagag 15300 aatttggaaa tccagcttcc cggactccat ttgtcgacgt atgccagcga agaaattgtt 15360 tctaaattcg atctcagctt ggacgtcacg gagatcgagg aaggcttgga atatctgttt 15420 gaatacgcca ctgctcttta taaaaccgaa acggtggaga aattggccgc tcactacttg 15480 cagctgcttg aatccattct ccgcaacccg tcggcgacta ttgccgagct gggcattttg 15540 acaccggcgg aaaaagagca aattctcggc gcgtttaacc cggcgcagcc gggagcggct 15600 cctgcggctg cgttccaccg gctgtttgag gaacaggcgg agcgcacgcc ggaagaggcg 15660 gccgtcgtgt acgagaacga ccggctgacg tatgcggagc tgaacgagcg ggcgaaccgc 15720 ttggcggcta cgctgcgcgc aagcggcatc ggccgggaga cgatcgtcgg cattctcgcc 15780 gagcgttcgg tggacttgct ggtggccgtg ctggccgtct ggaaagcggg cggggcgtat 15840 gtaccgctcg acccggatta tccggcagac cgcgtgcggt tcatgcttga agacagtgga 15900 gcgaaggtgc tgctgacgca aacgccgctg cgagagcgtg ccgaagcctg gctcgacgaa 15960 gaggagctgg cgctggcggc ggtgctgtac ctggacgacg aagcgtcgta cagcgaggag 16020 cgggcgaatg cgtcggttgt ctccggcatt gtctccggca ttgtctccgg caagctgacg 16080 gatgctgtgg acgacggcga tgagagccat cagaatgttg gcaccgacag cttccatgaa 16140 gcccgtccgg aggatctggc gtacgtgatc tatacgtcgg gaacgacggg caagccgaag 16200 ggcgtgatga tcgagcaccg cagcctggtg aacacggcgg cgggctaccg tcgggaatac 16260 cggttggatc agttcccagt gcgcctgctg cagctcgcaa gcttctcgtt tgacgtgttc 16320 gtgggagata tcgcccggac gctgtacaac ggaggtacga tggtgatcgt gccgaaggac 16380 gaccggatcg atccgtctcg tctgcaccac tggatggagc gggagcgggt caccatcttc 16440 gaatcgacgc cggcgctgat cgtgccgttc ctggagtacg tgcacgagca gcaactggat 16500 atgagctgga tggagctgtt gatcacgagc tcggacagct gcagcgtggc ggattaccgg 16560 accttgcagg aacgcttcgg ctcgttgttc cggatcatca acgcatacgg tgtgacggaa 16620 gctgcgatcg actcaagctt ctatgacgag gagctggcga agctgccgca gacaggcaat 16680 gtgccgatcg gcaaagcgtg gctgaatgcg aaattctaca tcgtggacgc gcatctgaac 16740 ccggtgccgg tcggagtgct gggcgagctg gccatcggcg gagtcggagt ggcgcgcggg 16800 tacttgaacc gtccggagct gacggaagag aagttcgtag acagcccgtt cgccgcgggc 16860 gagcggctgt accgcacggg agacttggcg cggtggatgg aggacgggaa cgtggacttc 16920 atcggccgga tcgacaacca ggcgaaaatc cgggggtacc ggattgagac gggcgaagtc 16980 gaagcgaagc tgctgagtgt aggtggcgtg aaggaagcgg tcgtcgtcgt cagggaagat 17040 caagaaggtc agaaagcttt gtgcgcttat tatacagcgg aagaaggcct gacggcggcg 17100 gacctgaaac gggcgattgc cagcgagctg ccggggtaca tgatcccgtc gtacttcgtc 17160 gagctggagc gcctgcctct gacgccgaac gggaaaatcg accggaaggc gctgccggcg 17220 ccggaagggg gagcaagcgc aggccgcgaa tacgtggcgc cgcgcaccga actggaggcg 17280 aaactggtcg ccatctggca ggacgtgctc gggccggtca cgattggcgt gacggacaac 17340 ttcttcgacc tcggcgggca ctccctgcgg gcgacgacgc tggtcagcaa ggtgcacaag 17400 gagctgagcg tggacctgcc gctgcgcgat gtgttccggc actcgaccat cgaagcgatg 17460 gccgaagcga tcagccaatt ggagcggcag gaacacctct ccattccggt tctggataag 17520 agggattact atccgctttc ctccgtgcaa aaacggctgt acatccagca gcagatggaa 17580 ggcgccgagc ttagctacaa catgtccggc atgacggttc tcgtcgggcg cttggaacgg 17640 aatcaattcg aggcggcgct caaaggattg atagctcgtc acgaaatttt gcgaaccggc 17700 ttcgaaatgg tcgacggcga accggtacaa cggatttatc cggacttgaa gtttgccgtc 17760 gagtatacga aagcgacgga aagtgaaacg aagagcatcg tagacggctt tgtgcgcgtc 17820 tttgatttgg agcggccgcc gctgctgcgt gtgggcttag tcgaatggga agcggaacgg 17880 catctgctca tgctggacat tcatcatatc gtcacggatg gcatgtcgat gggcatcttc 17940 gtcgaagagc tgctgcgcct gtataacggc gagactatgg aaccgcttcg gattcaatac 18000 aaggaattcg ccgcttggca gcagtccgaa cctgtaaaag agcggctgaa acgtcaggaa 18060 gcctactggc tggacgtgct ggaaggcgaa ctgccgacgc ttgaactgcc tacggacttt 18120 gtcagacctg ccgctcgcag ctttgaggga gatgtgctgc ctttcagcat cgacaagcag 18180 atgaccgaca gcttgcagcg catcgccgat gagaacggtg ccacccttta tatggtgtta 18240 ttggcggcct attcaatcct gctcagcaag tactcgggac aagaagactt cattgtaggc 18300 acgccggttt cgggccgcac acatgccgac ctggagccgc tcatcggaat gtttgtcaac 18360 actttggcga ttcgccatta tccgtccggg gagaagacgt tcctcgctta cttgaacgaa 18420 gtcaaagaaa cgatgctggg ggcctacgac caccaggatt atccgttcga ggagcttgtg 18480 aaaaagctgc aggcgccgcg agatcaaggc cgcaatcctg tattcgatgt catgtttgct 18540 ctggaaacca aggaagataa cgttcaaagc ttcggggata tcaagatcga atcttatccg 18600 gaaactcata cggtttccca atttgatctt accttggtca tttcgttgct ggatgaggga 18660 atgaacgggc agtttgaata tgccaccaag ttgtttacac gcaatctgat cgacaatttc 18720 gctcaggacc tgctcgtgat catcacccaa atttgcgaac agccttcggt gctgttgaag 18780 gatatttccc tgaacgggca atccgaacag gagcaagatg tgctagagac cattgatatt 18840 attttctaa 18849 <210> 10 <211> 1827 <212> DNA <213> Paenibacillus polymyxa F4 <400> 10 atggaagctg accgccagcc gtctgttcta gaacaaggca cagcggcgcc ttccaagcgc 60 caaaccgctt acgccgcctg gaaagcgttc cgctggctga tgtcctatgt aagccgtcac 120 aaaggctgga tgatcgtcgg tatcttgtcc gcgattgccg ccgccgttat tgagatatgg 180 acgggaagtt tgatcgagca gctgaccacc caggccgaga agggggcggg gccaatcgtt 240 ctgcaaatcg tgtacacggt ctttgtggtc atcttgatcg gtgtgccggc gaagtttttc 300 atgagcttcg gcgtggagcg aagcagcgcc tctgcggtgc aggatatccg caaccatgtc 360 atgcgtcata tcggcaaact cccggtctcc tatttggaaa agcagcactc cggcgacgta 420 ttgtcgcgga tcaacaacga cctgcagctc atccagcagt ttatgattcg ggacctcgcc 480 cagtggtttt atcatccgct gttattcatc ggctgtttcg cttatttgat ctacctccaa 540 tgggagctga tgctgtacag cctgctgtta tttcccgtag cgctgctggt ttcccaatgg 600 atcggcaagc agttggagcg gttgacggag gaagcccagg cgaacatggg ccgaatgaac 660 gtcaacctcc aggatacgct tgggggtatg cctattgtaa aaagctacct gctatccggc 720 atgttatctc gctcctacca agtgctgctg caattgacgg cccaaaaaaa gctggccgtg 780 aaaaagcggg aagcctgggt caacccgctg ctttccacgc tgatgatcag cccgatcatt 840 ttcgccgtca gttacggaag ctatttgatc tacaaagggc agctaggcgc gggagagctg 900 atcgccttcc tgtacttgct gaatctgtgt ctggagccgc tggagcatat tcccgagctc 960 atcacgcgga cgttcgaaat ggccggtgcc ctgagaaggg tctccgaaat cgtcgagcag 1020 ccgaccgaaa cggaaaatgg ccgttcgctt ccgaaagcga gcgccgcccc catcgagttt 1080 cagaacgtaa ccttcgggta tgaggagagc tccccgatcc tgcggaatgt tagcttctcg 1140 gtgccggaag ggaagacgat cgcgcttgtc ggagcgagcg gcggagggaa gagcacggtg 1200 tttaagcttg tatgcggctt ttatccgctt ccggaggacc agggcgagat ccgcgtgttc 1260 ggcagcctga tccacggcgc cgatccggag cagcttcggt cgcatttttc cgtggtaacc 1320 caggattcat atttgtttag cggcacgatc gctgaaaata tcggctacgg gcgggaagta 1380 gcgtcgatgg acgagattat cgaagccgcc aaagccgctc aggcgcattc cttcattatg 1440 cagcttcccg acggctacca gacgtatgtc ggagagcgcg gaggcttttt gtccggcggg 1500 cagcgccagc gcattgcaat ggcccgggct tttctgaagg atgctcccgt tctgctgctg 1560 gacgagccaa cgtcggctct tgatccggag tcggaaagcg cggttcagga agcgctgggc 1620 gtattgatga agcagagaac gacgatggtt attgcccacc ggctttctac agtacaaaac 1680 gccgacgaaa tttgggtcat ggaacagggg agtattgttg aaaagggcac tcatgagcaa 1740 ttgctgaaga tgaagggact gtacgcccag tcatactatc aggaatttac cgagtctgcc 1800 gaacgcaggg aggtggcgta cacatga 1827 <210> 11 <211> 1734 <212> DNA <213> Paenibacillus polymyxa F4 <400> 11 atgaaaaagg gcggatggct ctcgcaagtg aaagaactcg gctacttgct gatgtttatg 60 aaccgcaagc gcaaaaccca gtacgccatt ggcctggccg tgacggcgct cacccagaca 120 ttgttcctga tcgccttcag cttggtcgta cataacttgg ttgatttcgc cgtgtcccga 180 gatacgtccc tgatggtgga agcctttatt attttgggcg cggccttgtt cctggaaaat 240 gtgatttctc cctggttcat ttacttatac cagcgcagtg tcgagctgac tgtgctgaat 300 atccgcgaac gtctttatga caagctgtgc cgggtgcgac cgagattcct ggagcagacg 360 caccatgggg acttgctgtc gcgggtgaac aacgacgtaa cgaccgttga gtttacattc 420 tcgcaggttt acttcgtttt gctgcttcaa gttgtctttt gcatcggctc cattgtctcc 480 atggtgttga tcgattggcg gtttgccggc gtatccttcg tcattctgct gctgtcctcc 540 gtggtcagcc tgaaatttgc gcgggatatt cgcgccttgt ccgaacaagg cttgcaaacg 600 ctcggtaaaa tgaccgaaaa attcaaagat tttatgggcg gcattcaaat tgtgaagctg 660 ttccgcatcc gcacgattta cggccagtac gaggcgttga acgaacaaat gacgcagacg 720 cttcggcaaa ccgcgcagaa gaacggcacg caggctgcgg tgaatcattt tatcagctac 780 gtcacgttct gcggcatcat cgtcatcggc agtcttcttt atgcctacgg actgatggga 840 atgggaagcg tcgccgccct ggcggttctg caagtgaatc tgacgcacgc cttgttgaac 900 ttggggatgg ttctgtcgat gacccaaaat tcgctcgcgg gcgctcaccg gattcaagag 960 gtactaagag aggaagagga accggagcgt ttgggatctc ctcacagcga gctcgtgtcg 1020 gaggctgcgg tggagtttcg cgatgtggta ttttcctatc aggcggataa aaaggtgctt 1080 atcgacatct ccatgcaggt gtttcccggc caggtcgccg ctatcgtggg ggccagcggc 1140 agcggcaaaa gtacgctgat caagctgctg ctcggctttt atcctgtgga cagcggagaa 1200 atcctgctcc aaggcaaacc gttcggccat tacacgctgg acgaaatccg caggcagatt 1260 gcatacgttc cgcaggagcc gttcttgttt accggcacga ttgaggaaaa catccgctac 1320 ggcaacccgg atgcaacgga tgaagaagtg atcgaagcgg ccaaagcggc gtacgctcac 1380 catttcattc aggaacttcc tgaacagtat aaaacgccgg tgggagagag aggagcgtcg 1440 ttgtcgggcg gacaaaggca gcgaattgcg atcgcccggg cgattctcaa aaacgccccg 1500 attctgctgc tggacgaagc gacttccgcg ctggataacg aatcccagca ttgggtacag 1560 caggccctga acgaattgat gaaggggcgc accaccattc tgatcgccca ccgtctcagc 1620 accgtggaac atgcggattt gattaccgtc atgaaccaag ggacggtcgt cgagcggggc 1680 cgccatcagg acctgctggc gctcgggggg tattacgccc ggctgtacgg ctag 1734 <210> 12 <211> 14994 <212> DNA <213> Paenibacillus polymyxa ATCC21830 <400> 12 atgtttgaaa aggcggagag cgaggctgga gcgttgctgc tgcatatgct ccgccggatt 60 gcttttaccg atgaagagag gggaacgatc cagatggctt ttgaaaaaga aacgttgttt 120 tggaacgaaa aattcggtag cgacgattat accttgacgc ggctgcctta cagcaaagct 180 ccaagctccc tgacgcccat tatgacaacc gtcggcggga cgctttcgga ggaagtggcg 240 cagcgcgtcc ttcaaatgag caagggcgct ccgctggcca cttttatgat tttgctcgca 300 ggtgttcagt ccctcttgta taaatataca ggcgcttctg acattctggt cggcatgccg 360 gttgtacgga aaccgacgga gacgcgccga tccgttaatc atacgatcat tttgaaaaac 420 tcgctttcgg cgggtgcgac ttttaaaacg cttctgagcg agttgaggac ttccttgccg 480 gaagcgattc agcatcaaca tattccgttc ttgaaaatga cggagaagct ggatctgcaa 540 tatgcagacg ggatacccgt cgtccatacg ctagtatccc ttaaggagct gcacctggac 600 gaaatcggac aaaacgtggt cacggattgt tcctttgaat tcagcttaac gggcgggacg 660 atacagctag cgttgtcata taacgagcac ttatatgact ccgagttcat gactcgggtc 720 gttggccatc tgaatcgact gttggccgtg gggcttcacg agttggagct ggagatcgtg 780 cgggcggaca tgctgtcgga ggacgagaaa tttcaattgt tgcaaagctt taacgatacc 840 gagaaggact atccccgtga tcggacgatt catcaactcg tggaggagca ggcgaagcgg 900 gtgcccgaag cgacggcggt tgtctttgag ggtcggcggc tttcgtacgc ggagctgaac 960 gaacgggcga accggctggc gcggacgctg cgatcggtcg gcgtgctgcc caatcagctg 1020 gtaggcttga tggccaggag atcactggag acggtcgttg gcatactggc ggttctgaaa 1080 gctggcggtg cctacgtgcc gatcgacccg gaatacccgg aagaacgcat ccgctacatt 1140 ctggagaact cgaacgcgca gctgctgctg actcaaaggg agctgctgca gcaggtgccg 1200 ttcgaaggaa ccgtgttggc gctggatgac gaacagacct acagcgacga tggctcgaac 1260 ttggagccgg ccagcggtcc gaatgatctt gcttatgtca tctatacgtc aggtacgacg 1320 ggcaaaccca aaggggtcat gctggagcat cgcggtttgg tcagcttgaa gctgatgttc 1380 gcggataggc taggcatcac ggagcatgac cggatcgttc aattcgccag cctgtcgttc 1440 gacgcgtcct gctgggaaat gttcaaagcg ctctattttg gcgcggcttt gtatatcccg 1500 acggccgaga cgattctcga cacccgcttg ttcgagagtt atatgaacga gcatgcgatt 1560 acggcggcga ttttgcctcc aacgtacagc gcttatttga acccggaccg ccttcccagc 1620 ttaacgaagc tcgtaacggg aggctctgcg gtatcggccg aattcgtgca gcaatggaaa 1680 ccgaaggtcc actatttcaa tgcttacggc cctaccgaag cttcgattgt tacgacgctt 1740 tgggatgcgg atgaggagca gtcggagcgc agagtcattc cgatcgggcg cccgctggcc 1800 aatcaccgga tttttatttt ggatgcccac ctgcagcttg tgcctccggg agtggacggc 1860 gagctgtgcg tggccggcgt ggggcttgcg agaggttacc tgaaccatcc ggagctgacg 1920 gcagagaagt tcgtggaaca tccgtttgcg ccgggagaac gcctttaccg gacgggagat 1980 ctcgcccgct ggctgccgga cggaaatatt gagtacttgg gccggatcga tcatcaggtg 2040 aaaatccgtg gattccggat cgagatcggc gagattgaag agcaacttct gaagatcgac 2100 tccgtgcagg agacgatcgt aatcgcgcgg gaaggcaaaa gtggacaaga actgtgcgct 2160 tacctggtcg cgggccaccc gcttacgctc ggcgagttga gaagcgcgct ggcgcaaaaa 2220 ttgccgaatt acatgattcc ggcgcatttt gttcagcttc cgcggatgcc gctcacgccg 2280 aacgacaaaa tcgaccgcaa ggctttgccc gtcccggaag gaaacgcact gaccggcggc 2340 gcgtacgtag ctccccgcaa tgaagccgag cggacgcttg ccgatgtgtg gcaggcggta 2400 ttgaacgccg atcgcgttgg ggtaacggat catttcttcg agctgggcgg agactcgatc 2460 aagtccattc aagtatcttc gcggcttcat caagccgggt acaagctgga aatccgggat 2520 ttgttcaaat atccgactat ctcacagctc agtctgcatg tgaaaccgat cggacgtacc 2580 atcgatcaag gcgaaataac gggtgaaacg gcgctgacgc cgattcagca ttggtttttc 2640 gagagctcct ttgcggaccc gcatcatttc aaccagtcgg tgatactgta ccggaaggaa 2700 cgcttcgacg aagagacggt gcgtcaggtg ctgcaaaagc tggccgagca tcatgatgcc 2760 ttgcggatgg tgttccgcaa aacggaacaa gggtttagcg cgcggaaccg cgcgattcaa 2820 gaaggcgggc tgttcacgct ggacgtgttc gacttcaagg atgcaaagaa taccgcgcag 2880 gctgtggaag cgaaggcaac ggacattcaa gcgggcatcg atctggagaa cgggcccctc 2940 gtgaaggcag gactgttccg atgcgcggac ggcgatcatt tgctgctcgc ggttcatcat 3000 gccgtggtgg acggcgtgtc ttggcgtatt ttgatggagg atttcgccct gggttacgag 3060 caggccggca aaagtgagga aattcgtttc ccggcgaaaa cggatgcgta ccgcacttgg 3120 tccgagcagt tggccgctta cgtgcaaagc ccggagatgg cgaaggaacg ggcctattgg 3180 caggccgtgg aacaaattgc ggttccggcc gtgccgaagg atctggaggc ggacgttacg 3240 acgcagcagg acagcgaatc gctgttcgtc tgtttgactc ccgaagaaac ggaactgctg 3300 ctgaagcggg ttcaccgggc ctacaacacc gaaatgaacg atattttggt aacggcgctc 3360 ggcatagcca ttcgcaagtg gacgggacac gaacgggtgc ggatcaatct cgaaggacac 3420 ggacgcgaat cgatcggaac ggatatcgac atcacgcgca cagtcggctg gtttacgacc 3480 aagtttccgg tcgtcctgga gccggaaacc gaccgggatt tggcctatca gattaaacag 3540 gtcaaggaaa gcttgcgccg cattccgaac aaagggcttg ggtacggcgt atgccgctat 3600 ctctccaaat cggaggatgg ctttgtttgg ggtgcagagc cggaaattaa ttttaactac 3660 ctcggccagt tcgacgatga tgtcaaccag gacgagatcg gcatatcttc ttattccagc 3720 ggcagcccgg ccagcgaccg gcaggcccgc agctttgtgc tggatatcaa cggcatggtg 3780 ctggacggcg ctttatcgct cgatctcagc tacagccgga agcagtatcg caaggaaacg 3840 atagaagcct tcgctcagcg gcttgagcaa agtctccgag agctcattac ccactgcgca 3900 ggcaaagaaa acaccgaatt gacgccgagc gacgtgcaat ttaaaggctt gaccatcgcg 3960 gaattggagc aaatcgccca gcgctcgggc catctcgggg aaatcgaaaa tatttactcg 4020 cttacgccga tgcagaaggg catgtggttc cacagcgcgc ttgaccggca gacggccgct 4080 tacttcgagc aaacgcggtt tacgatgcgg ggagcactcg acgtccagct tttcgagagg 4140 agctggatgg agcttgcgaa acgtcatctg gtgctgcggg caaattttgt gaaaggaccg 4200 gcgggcgagc cgctgcaaat catataccgc gacaagccgg tcggctttga atatgaagag 4260 ctgctccatt tgcaggcgga cgagaaacaa acgtacttgg ataaaaaggc cgaggatgac 4320 aagcttcgcg gcttcgacct ggaacatgac gcgctcgttc gggttacgat cctgcgcacc 4380 gaagagcaaa gctatcatgt gctgtggagt ttccagcata ttttgatgga cggctggtgc 4440 ctgccgcagc tgacgcagga gctgtttgag acgtattcgg ccttggcatc cggcaagcag 4500 ccagcgggag ataagggatc ggattatggc gcttatatcg aatggttgga gaaacaggac 4560 gatcaggcgg catccggcta ttggacggca ttcctggcag gttatgaagg gcaaaccgta 4620 ctcccaggac aaaaggaacc ggcgccaaac ggcagattta cggctgatca cgtcaccgcc 4680 gagctcggca aggacttgag cgagcgaatg gaccgggtgg cgaaacagcg cctggttacg 4740 gtcaatacgc tgctgcaagc cgcttggggc gtgatgctgc aaaaatataa cgggacaaac 4800 gatgccgtat tcggcagcgt cgtagccgga agaccggcgg aaatcccggg catcgagtcc 4860 atgatcgggc tgttcatcaa tacggtgccg gtccgcgtca cgagcgaagc ggacaccgtg 4920 ttcgccgacc tgatggcgaa gctccaagag cgggcgctgg agtctgggcg ttatgattac 4980 tatccgctgt atgaaattca agcccacagc gtgcaaaagc agaacctgat caaccatatc 5040 atcgctttcg agaactatcc ggtggacgag cagatggagc aggcgggcga ccagcagcac 5100 ggcgacctga cgatcgctga cgttcagatg gaggagcaga cgaactataa cttcaacgtg 5160 accgtggtgc cgggagacga gatcgaaatt cggttcgact ttaacgcaga ggtgttcgat 5220 aaagacagca tcgagcggct caaggggcat ctcgtccatc tgctggagca ggtgacggat 5280 aacccggaaa ttacagtagg cgagctggaa cttgtgacgg aggcggaaaa ggccgacctt 5340 ctcggacgtt ttaacgacac taccacggaa tttccgcgcg ggaagacgct cattcaattg 5400 ttcgaagagc aggcggagcg catcccggat gcggctgcca tctccttgaa tgagcaagag 5460 ctgacctacc gcgagctgaa cgaacgcgtc aatcgccttg cccgtacctt gcgtagccac 5520 gggatatcca aaggccgtct ggtcgccatt atggctgagc gttccatcga aatggtggtg 5580 ggtatgctgg cggcacacaa agccggagcg gcttacgtac cgattgaccc ggaatatccc 5640 gaggagcgta tccgtttctt gatcgaggat tcgggagcgc aggtcatgct gacgcaaagc 5700 cgcttgcgcg agcgcctggc gggttcggac tccgtgatct tactggatga cgagtccttc 5760 tatcacgagg acggcacaaa tcttaatccg ggcaccgaag cgacagatct ggcctgcgtc 5820 atctatacgt caggtacgac gggcaagccg aaaggcaacc ttgtttcgca ccgcaacatc 5880 gtgcggatcg tgcgaaatac gaattatatc gacatcaccg agcgggatca tgtcctccag 5940 ctttcaagct attcgttcga cggagcgatt ttcgatgttt tcggcgcttt gaccaatggg 6000 gcgcggctgg tgctggttcc ccgcgagact ttgctggaaa tcggccggct ggcggatctc 6060 atccagcacg agcgcatctc ggtcatgctg attacgacgg cttacttcaa cgtccttgta 6120 gatgtgaacg tcgactgcct gcgggatgtc cgggcgattt tgtttggagg agagcgcgtg 6180 tcggtcggcc atgtgcgtcg agcgctcgcc catatcggac cgggcaggct taaccacctg 6240 tacggcccgt cggaaagcac ggtttatacc acgtatcttc cggtcgactt tgtcgatgag 6300 tcggcggtta ccgtacccat cggacggccg atcagcaata caacggtgta tatcgtcgac 6360 agccggaata agcttctgcc gatcggcgtg gctggagagc tttgcgtcgg cggagaaggc 6420 ttggtacggg gctacaacaa ccggccggag ctgacggcgg agaaatttgt cgacaatccg 6480 tttgtgccgg gagagcgcat gtaccgaacg ggggatttgg ccaaatggct gccggacggc 6540 acgatcgaat acgtgggacg gacggacgac caagtgaaaa tccgcggctt ccgcattgaa 6600 ctgggcgaga tcgaagctca gcttcagaaa gtggagggaa ttcggaaaac gacggtattc 6660 gcgagggaaa acgcctccgg cgagaagcag ctttgcgcct attatgaagc ggaccgcgag 6720 ctttcagcgg ccgagttgaa gaacgtgatt tcccaggaac tgccggccta catgatcccg 6780 gcgtacctga tccagttgga gcggctcccg ctgacggcga acggcaaggt cgaccgccga 6840 tcccttccga cgccggagga gagcttgcag ccgggcgaag gacgtactcc gcctcgtact 6900 ccgctggaag ccagcttggc cggaatttgg aaaagcgtac tcgcattgga gcacatcggc 6960 gttcatgaca acttcttcga cctgggcggc cattccctgc gggcgacgac actggtgagc 7020 aaggtgcatc aggagctgaa cgtcgagctg cctctgcgcg acgtattccg ctactcgacg 7080 atcgaagaga tggctctcgc catcacccgg atcggagagc agtcattctc gtcgattccg 7140 ctggcaggcg taagagaata ttatccgctt tcctcagctc agaagcggct gtttatcctg 7200 aatcagctag aagggtccga tcagagctac aacatgccgg gcgtgctgct gctggaagga 7260 tcgattgacc ggagcctgct ggagaaggct ttccgcggac tgatcgtacg gcacgaaacg 7320 ctacgaaccg gctttgagat cgtacaaggc gaagcagtac agcgcattta cgagagcgtc 7380 gactttgccg tcgagtaccg tcatgcgagc gaggaagaag cgcctgaagt cgtgcaggcc 7440 ttcatccgac ctttcgactt ggcgaagcct ccgctgctgc gggtggagct cgtagagctc 7500 gcaaccgaac gttatttgct gatgttcgac atgcaccata tcgtctccga cggggtttcg 7560 atggacgtgt tagtcgagga actcgttcgt ctgtacggtg gcgagtcatt agagcctttg 7620 cgcattcaat acaaggacta tgcggtatgg cagcagtcgg acgagcaaaa agcgcagttg 7680 aaacgcgagg aagcttactg gctggaccgt taccggggtg aactgccggt tctggaaatg 7740 ccgacggact atccgcgtcc tgccgtgcag agctttgagg gacaaacgct gacgtccttc 7800 gtggacgagg caacgaatga aggcttgaag caactggccg ctcaaagagg aacgacgctg 7860 tatatggtgc tgcttgcggc atatacagtg cttttgcata aatacacagg tcaggacgat 7920 ttgatcgtcg gaacgtcgat tgcgggcaga acgcacggag acacgcagcc tttgatcgga 7980 atgttcgtca atacgctggc gctccgcaat tatccggctt cggagaagac cttcctgtcg 8040 tatctggaag aagtgaaaga aacgaccttg ggtgcttacg agcatcagaa ttatccgttc 8100 gaagagcttg tcgataaagt gcaggtcagc cgggatttga gccgcaatcc gctgtttgac 8160 acgatgttct ccctgcaaaa cttggaggat aaagagtttg agctggaagg gctgaaattg 8220 tccccgtacc ctagcgaata cggtacggcc aagttcgacc tgagtgtgga tgttacggaa 8280 gaaaacggcg gcctggagtg cagctttgaa ttcgcaacgg ctctttataa agaaagcacg 8340 atccggcggt tgtcgactca tttcggacat ttgcttgcgg agatcgtaag ctgtccggat 8400 gcgaagattg ccgagctgaa cttgctgacg gcagaggaaa aagaacaaat tctcggcgcg 8460 tttaacccgg cgcagccgga agtggctcct gcggccgcgt tccaccggct gtttgaggaa 8520 caggcggagc gcacgccgga agcggcggcc gtcgtgtacg agaacgaccg gctgacgtat 8580 gcggagctga acgagcgggc gaaccgcttg gcggccacgc tgcgcgcaag cggcatcggc 8640 cgggagacga tcgtcggcat tctcgccgag cgttcggtgg acttgctggt ggccgtgctg 8700 gccgtctgga aagcgggcgg ggcgtatgtg ccgctcgacc cggattaccc ggcggagcgc 8760 gtgcggttca tgcttgaaga cagcggagcg aaggtactgc tgacgcaaac ggcgctgcga 8820 gagcgtgccg aagcctggct cggcgaagag gaactggcgc tggcggcggt gctgtacctg 8880 gacgacgaag cgtcgtacag cgaggagcgg gcgaatgcgc cgattgtctt cggcatttcc 8940 agcaagctga cggatgctgt ggacgacggt gatgagagcc ctcagaatgt tggcaccgac 9000 agcttccatg aagcccgtcc ggaggatctg gcttacgtaa tctacacgtc gggaacgacg 9060 ggcaagccga agggcgtgat gatcgagcac cgcagcctgg tgaacacggc ggcgggctac 9120 cggcaggaat accggttgga tcagttccca gtgcggctgc tgcagctcgc aagcttctcg 9180 tttgacgtgt tcgtgggaga tatcgcgcgg acgctgtata acggaggcac gatggtgatt 9240 gtgccgaagg acgaccggat cgatccgtct cgtctgcacc attggattga gcaggagcgg 9300 gtcaccatct tcgaatcgac gccggcgctg atcgtgccgt tcctggagta cgtgcacgaa 9360 catcggctgg atatgagctg gatggagctg ttgatcacga gctcagacag ctgcagcgtg 9420 gcggattacc ggaccttgca ggaacgcttc ggctcgttgt tccggatcgt caacgcatac 9480 ggcgtgacgg aagcggcgat cgactccagc ttctacgacg agccgctggc gaagctgccg 9540 cagacaggcc atgtgccgat cggcaaagcg tggctgaatg cgaaattcta catcgtggat 9600 gcgcatctga atccggtgcc ggtcggggtg ctgggcgagc tggtcatcgg cggagtcgga 9660 gtggcgcgcg ggtacttgaa ccgtccggag ctgacggaag agaagttcgt agacagtccg 9720 ttcgccgcgg gcgagcggtt gtaccgcacg ggagacttgg cgcggtggat ggaagacggc 9780 aacgtggact tcatcggccg gatcgacaac caggcgaaaa tccgggggta ccggattgag 9840 acgggcgaga tcgagtcgca gctgctgcgg gtgaaaggcg tgcgcgaagc ggtggtactg 9900 gttctaagtg acgcgaacgg gcagaaggcg ctgtgcgcgt attacacgcc ggataccgga 9960 gcggagctgg cagtgaacga tctgcgcagc acgctggcgc aggagttgcc ggggtacatg 10020 atcccgtcgt acttcgtgga gctggagcac ctgcctctga cgccgaacgg aaagattgac 10080 cggaaggcgc tgccggcgcc ggaaggggaa gcgggaaacg gaacggagta cgtcgcaccg 10140 cgcaatgagc tggaaacaaa gctggcggcg atttggcagg aggtactggg gcttgcgaag 10200 gagattggcg ttcacgacaa cttcttcgac atcggcggcc actccctgcg ggcgacgacg 10260 ctggtcagca agattcataa agagttgaac gtggatctgc cgctgcgcga cgtgttccgc 10320 cattccacca tcgagagcat ggcggccgcc atttcccggc tggatgagca gacattcgtt 10380 tccataccgg tggcggatga ccgagaggtg tacccgcaat cttttgctca aaaacgtctc 10440 tttatcctga atcaactgga aggcgcggag cttagctaca acatgccgga agcgatgctg 10500 ctggagggtg ctttggaccg ggcaaggttc gaagaagcgt tccgtaagct cgtgacgcgg 10560 catgaaacgc tgcgcaccgg gttcgaaatg gtggatggcg aagcatcgca gcggatttat 10620 caggacgtga atgttgccgt ggagttctat cgagcggata agcaagaggc cgaagagacg 10680 gttcgtcgtt tcgtccgtcc gtttgacttg gcgaagcctc cgctgctgag ggtaggcctt 10740 gtcgagctgg ctccggaacg ccatattcta atgtacgaca tgcatcatat tatttccgac 10800 ggcgtctcga tggaaatctt tgttgaagaa ttcgtccgct tgtacggcgg cgagcaattg 10860 gatcctctgc gcattcagta caaagactac accgtttggc agcattcgca ggagcagaag 10920 gaacggcttc agcgccagga ggcatactgg ctggacatgt tccaaggcga gcttccggtg 10980 ctggaaatgc cgaccgacta tccgcgtccg gccgtgcaga gctacgaagg ccaaacgctg 11040 gagtttttct tcgacgcttc gaaaaccgac ggcctgaagc agctggcctc ggaaacgggc 11100 acgacgctgt ttatggtgct gcttgcggcg tataacgtcc ttttgcataa atattcaggt 11160 caggaagacg tgatcgtcgg tacaccgatt gctggaagga atcatggaga tgtacagccg 11220 ttgatcggga ttttcttaaa cacgctggct atccgcagtt atccggcttc ggagaagaca 11280 ttcctgtcat acctgaacga agtcaaagaa acgaccctcc acgccttcga gcatcaaaac 11340 tatccgttcg aagaattggt ggacaaggtg caagtcaccc gtgatttaag ccgcaatccg 11400 ctcttcgaca cgctgtttac gatgcagaat acggagaacg aggaatttga gctggaaggg 11460 cttcgcctga ttccttatcc gagcgcactg gataccgcga agtttgatat cagcttggat 11520 gtgggcgagg agaacggcgg cttggattac agcttcgaat atgcgacggc tctctacaaa 11580 agggagacga tcgaacggct ggcgaaacat tacgaacagc tgctcgtaac gatcgtaagc 11640 cgtccggatg cgaagatcgc cgagctgaat ttgctgacgg cagaggaaaa agaacaaatt 11700 ctgggcgcgt tcaacccggc gcagccggaa gcgacttctg cggctgcgtt ccaccggctg 11760 ttcgaagaac aagcggagcg cacgccggaa acggcagctg tcgtgtacga gaatgaccgg 11820 ctgacgtatg cagagctgaa cgagcgggcg aaccgcttgg cggccacgct gcgcacaagc 11880 ggcatcggcc gggagacgat cgtcggcatt ctcgccgagc gttcggtgga cttgctggtg 11940 gccgtgctgg ccgtctggaa agcgggcggg gcgtatgtgc cgctcgaccc ggattacccg 12000 gctgagcgcg tgcggttcat gctcgaagac agcggagcga aggtgctgct gacgcaaacg 12060 gcgctgcgag agcgcgccga agcctggctc ggcgaagagg agctggcgct ggcggcggtg 12120 ctgtacctgg acgacgaaac ttcgtacagc gaggagcggg cgaatgcgcc gattgtctcc 12180 ggcatggtct ccggcaagct gacggatgct gtggacgacg gcgatgagag ccatcagaat 12240 gttggcaccg acagcttcca tgaagcccgt ccggaggatc tggcatacgt gatctatacg 12300 tcgggaacga cgggcaagcc gaagggcgtg atgatcgagc accgcagcct ggtgaacacg 12360 gcggcgggct accggcggaa ataccggttg gatcagttcc cagtgcggct gctgcagctc 12420 gccagcttct cgtttgacgt gttcgtggga gatatcgcgc ggacgctgta taacggaggc 12480 acgatggtga ttgtgccgaa ggacgaccgg attgatccgt ctcgtctgca ccattggatt 12540 gagcaggagc gggtcaccat cttcgaatcg acgccggcgc tgatcgtgcc gttcctggag 12600 tacgtgcacg aacatcggct ggatatgagc tggatggagc tgttgatcac gaactcagac 12660 agctgcagcg tggcggatta ccggaccttg caggaacgct tcggctcgtt gttccggatc 12720 gtcaacgcat acggcgtgac agaagcggcg atcgactcca gtttctacga cgaggaactg 12780 gcgaagctgc cacagacagg caatgtgccg atcggcaaag cgtggctgaa tgcgaaattc 12840 tacatcgtgg acgcgcatct gaacccggtg ccggtcgggg tgctgggcga actggtcatc 12900 ggcggaatcg gagtggcgcg cgggtacttg aaccgtccgg agctgacgga agagaagttc 12960 gtagacagcc cgttcgccgc gggcgagcgg ctgtaccgca cgggagactt ggcgcggtgg 13020 atggaagacg gcaacgtgga cttcatcggc cggatcgaca accaggcgaa aatccggggg 13080 taccggattg agacgggcga gatcgagtcg cagctgctgc gggtggaagg cgtgcgcgaa 13140 gcggtggtgc tggttcgaag tgatgcgaac gggcagaagg cgctatgcgc gtattacacg 13200 ctggataccg gagcggagct ggcggtgaac gatttgcgca gcgcgctggc gcaggagctg 13260 ccggggtaca tgatcccatc gtacttcgtg gagctggggc gcctgcctct gacgccgaac 13320 ggaaagattg accggaaggc gctgccggcg ccggaagggg aagcgggaag cggaacggag 13380 tacgtcgcac cgcgcaatga gctggaaacg aagttggcgg cgatttggca ggaggtgctg 13440 gggcttgcga aggagattgg cgttcacgac aacttcttcg acatcggcgg ccactccctg 13500 cgggcgacga cgctggcggg caaggtattt aaggaattaa acgtcaacct gccgctgcgc 13560 gacgtattcc gtcactcgac gattgcggcg atggctgagg cgatcgcccg gatggaacgg 13620 cgggagcatg aggctattcc tcaagcggag gagagagagt actaccctct gtcctccgcg 13680 cagaaacggc tgttcattca gcatacgctg gatggagcgg atcagcttta caacatgccg 13740 gagttggtgc aggtggaagg cgagtttgat ttagaccggt tggaagccgc cttgcggaaa 13800 ttgataacac ggcatgaatc gctgcgcacc ggttttgaaa tcgtgaaggg cgaagcggtt 13860 cagcgggttt acccgcaggt cgattttgct atcgagcatc atcaagcgga taaagaggat 13920 gcggctcaaa tcgagcagat cgtccgcagc ttcattcgtc cgtttgatct cggcaagccg 13980 ccgctgctgc gcgccggggt catcgagctg gagccgaacc tgcatattct ccttttcgac 14040 atgcaccata tggtgtctga cggcgtatcg atggcgattg tgatcgatga gttctcaagt 14100 ttctacgccg gggaagaact gccgccactg cgcattcaat acaaggatta tgccgtttgg 14160 cagcagtcga aggcccaccg agagcggatc gggcggcagg aagcgtactg gctgcaaacc 14220 ttcgaaggcg agctgccgac ggcggacctg ccgatcgact acgaacggtc cgcggctcgc 14280 agctacgaag gcgcgcatct ggagttcaac gtcgaagctt ctctctctgc gcggctgcgc 14340 gaattggcgg ccgagcgtga aagcacactg ttcatggtgc tgcttgcggc ttataccgtg 14400 ctgctgtcca agtacagcgg gcaggaggac ttgatcgtgg gtaccccggt ggcgggaaga 14460 acgaacgccg atttggaatc ggtcatcggg atgtttgtca atacgctggc gatccgcaat 14520 cgtccttcgg gcgacaagac gttcttgtcc tacctggaag aagtgaagga aacggctttg 14580 ggtgctttcg agaaccagga ctatccattc gaggagctcg tggagcgttt gaatgtgaag 14640 cgggagccgg gccgcttccc gctgttcgat gccgttttcg acttgcaaaa tatcgaagaa 14700 cgagacgccg agctggaagg ggtcagcctg aagacttacg agcttgacca tttagaagaa 14760 gcgaagttcg atctgacact gtttatgtat gaaaacaacg gggcgctgag cgggggcttc 14820 ttctacgcca ccaagctgtt caaagaagcg atgatccgca ccttgaccga ggattacctg 14880 cgggtactgt ctcaaattgc ggaaaatcca caactcgagc taagccggat tgaatgccat 14940 aaaccggcgg caggcgcaaa gagtgccgtc gatacgatcg aattcgcgtt ctaa 14994 <210> 13 <211> 3309 <212> DNA <213> Paenibacillus polymyxa ATCC21830 <400> 13 atgaaatctt tgtttgaaaa ggaagaacgg tactggagcg gcaagtttga cgccgatgac 60 agcctgagct tccttcccta cagtcaatcc tccaaattat cggccgacgg ggaagctgcg 120 gccgagccgg gtttgcttca ccgtactctg ccgagcgaaa tctcggagag aatcatttgc 180 cttgccaacg gttcggattt ggctttgtac atgattgttt tagcaggagt aaaaagcctg 240 ctgttcaaat ataccgggcg ggaccaagtg ctggtcggga tgccctctta tagcgcagac 300 cccgacggga ctccgccgcc gcatgacatc ttggtgatca agacgtccgt aagccgcgag 360 actacgctta gaacgctgct cgggggcatc aaagcctcca tcggcgaggc gctggagcat 420 cagcacctgc cttttcggaa aatggtggag ccgctccatc tggactatac gggggacggt 480 ctcccggtcg tcaacaccgt cgcatccttc gccccgattc atccggaacc gctgggtaac 540 cgggtggcgg ccgatacggt ttttcgcttc gaccgccaaa accactccat cgagctggaa 600 ataagctttg acgggcagcg gtacgagcgg gcatttgtag aacaggcggc cgaccatctt 660 gttcagctgc tgtccgtgct tttatttcag ccggatctgg agcttggaca agccgatgtg 720 ctgtccccag acgagaggga gacgctgctg aagcgattta atgacaccga aaccgggttc 780 gagcggggga aaacgattta cggcttgttc gaagaacagg cggagcttta cccggacaac 840 gtggccgccg tcatgaacga gcggcagctg acctaccgcg agctgaacga gcgatccaac 900 cgccttgcgc ggaagctgcg ggagacggga gtagaagcgg accagctggt agcgattctg 960 gccgaacgct cgctcgatat ggtcgtcggc attctggcga ttctcaaagc gggcggagca 1020 tacgtgcctg tcgatcccga ctacccggag gagcgcatcc gcttcatgat cgaggattcg 1080 ggtgcgccgt tattgctgat tcaaaagcat ctgcacgaga agaccgactt cgcaggaacg 1140 cgcctcgaat tggacgattt cgtttggggt gacagagggg cggactccga aggagcgctg 1200 gacgcttcga acctggagcc gatttctggg ccagtcaacc tggcttatgt catctacacg 1260 tcgggaacga ccggcagacc aaaaggaacg ctgatcgagc ataagaacgt cgtgcgcctc 1320 ctgttcaacg acaagaacct attcgacttc gggccgtccg acacgtggac gctgttccac 1380 tcgttctgct tcgatttctc cgtctgggaa atgtacggag cgctgctgaa cggaggcaag 1440 ctggtcatcg taccgccgct cacagcgaaa aatccggccg atttcctggc gctgctgggc 1500 cgcgaacagg tcacgatttt gaaccagacg ccaacgtact tctaccagct gctgcgtaag 1560 gtcttggcgg accatccgta cgatttgcgg attcgcaacg tcatcttcgg gggcgaagcg 1620 ctgagtccgc tgctgctcaa gggcttcaag acgaagtacc cggagacgaa gctgatcaat 1680 atgtacggca ttaccgagac gacggttcac gttacgtata aggaaatcac gtgggtcgaa 1740 atggaggcgg cgaagagcaa tatcggcaag ccgatcccta cgctgagggt gtacgtcctt 1800 gatgaaaacc gccggcctgt gccgatcggc gtagcgggcg aaatgtacgt ggccggggca 1860 ggccttgcga gaggatacct gaaccgtccg gatctgacgg cggagaagtt cgtcgattcc 1920 ccgtttgcgg agggggagaa actgtaccgc tcgggtgact tggcggcttg gctgccggac 1980 ggcaacatcg aatacctggg ccggatcgat catcaggtga aaatccgcgg gtaccggatc 2040 gagctggacg aaatcgagac gcagctgctg aagatcgccg ccgttcaaga agccaaggtg 2100 ctcgaccgcg acgacgcaaa cggccaaaag cagcttgtcg cttactacgt cgcggaaacg 2160 aggctggcgg tgcatgaact caaggaggag ctcgccaagc agcttccggg gtatatgatt 2220 ccttcgcacc tcgtgcagct tccgcggatg ccgctgaccc cgaacgggaa aatcgaccgc 2280 aaagcgctgc ccgcgccgga ggaagccgcg gcccgaggag cggaatatgt cgcgccgaga 2340 acgctgctcg aaatgaagat cgcccgcgtc tggcaggata cgcttggcgt tccgcaggtc 2400 ggcgtaaagg ataacttttt tgagttgggt ggcaattcgt taagtctgat gaggctcgtt 2460 caagccgttt acgatgaaac gggcattgag ataccgctaa accgccaatt ccataatgta 2520 acagttgaag ccatggcttt cggagagggg gacctgggcc tggataaagg gggagactcc 2580 ttcattaagc tgaataaagt aggaaatctg aatgtgttct gcttccctcc gggcagcggc 2640 ttcggcatcg gttaccgaga gctcgcaagc aggctcgacg gtcggtccgt gctctacagc 2700 attgatttta tcgacgatgc cgtcgattac gaggccatgc tgaaccgtta tgtggacgag 2760 atcgtccgca tccagccgga aggaccttac gtgctgctcg gctactgctt cggaggaaac 2820 ctgacgttcg aggtagccaa aacgatggag aaaagagggt attccgtaac agacgtgctt 2880 atggtggact cgtggattaa gcacacgctg acgccttccg aaacgtcgga gaaagagctt 2940 gaagaaacgc ttgccgattt cgacgaagaa gagaaggaat taatgagcaa cccgctcgta 3000 cgggagcggg ttcatcggaa ggtcaaagcg accttggcgt acgaagcgca gctcattaat 3060 tccggcacga tcccggcccg gatttacgaa ctgattgcga aggacagcga agcgttccgc 3120 ctggagcacc aattgccttc ctggcggggg gcaacgacgc aagcttacgc cgattaccgg 3180 ctggagggcg cgcacgagga attgctggaa ctcgcgcgcg tggacgaaac ggccgtcgtc 3240 atccgggaca tcttagagca agtcaagcgg cagattgaag cggaggccgg tgtactgcat 3300 ggaagctga 3309 <210> 14 <211> 17370 <212> DNA <213> Paenibacillus polymyxa ATCC21830 <400> 14 gtgagagagg ataccaacga gcaatatgga ttaacgcaag cccagcgccg aatatggttc 60 atggaaatta tgaatccggg aacgtccatc acgatgcttt ccgcgaccta ccagattacg 120 ggcgagatca acacacagct tctgaagcaa gcggcggcag agatcgtcaa aacctatgac 180 gttttccgaa tccgcattag cggggatttg caaaatccaa cgcagtggtt cgaagagccg 240 gagaatgtcc aagctaggat aagccgcctc gaaataggca caaccgaaca attctatgct 300 tgggtgaaag aagtaagcga aaaaccggcc agcgtgttcg acgaacacct ctaccaattt 360 acgattatcc attttgcgaa cggccaagta tggctcaatt tgacggtaaa tcatattatc 420 gccgacggct tgtccgtcaa tgctttgctg catgcggtga tggaaaagta cctggaactg 480 cgcaaaggca tctccagcag ttaccagccc ccttcctatc tagattatat ttcctcggag 540 cgtgaatatg agcaatcgca gcgttatcaa aaaggcaagg aatactggct gacaaagtac 600 aacactttgc ctgaaacgac cggcattaaa tcgtatccgc cattctcgat cggcagcgaa 660 tccaataaac tgtccatcac tttggacggt tcccggtatg aacgcattct ggccttctgc 720 gaacaatatc aggtcagctt atatacgtta tttctgtccg ccatgtacgc cttattgtac 780 aaactgaccg acagcaccga tgttccggtc ggcacggttt tcgccaatcg caccagcaag 840 aaggaaaaag aaacgatcgg catgttcgtc agcaccgtgg ctacgcggat ttgtctgaat 900 ccagacgggc acgtgctttc cttgatccaa acggtttcca aggaaaatac ggcggatctg 960 cggtatcaga aatatcctta taaccaattg atccaggatt tacgcgaaca acacggccgc 1020 aacgatcttt cggggctgtt ccgcacgtct ctggaatatc tgcctttgaa aatcgtggag 1080 tacgaagaaa tcaaggtacg cttggaggct cactttgcta ggcacgagat ggacgatttg 1140 ctgctgcgct tcgaccatat gctgaatgaa agccatgtaa ttctccatgc ttcctatcgt 1200 accggcctgt tcgagaaggc cgagattgat cggattatgg aacagtacgt aaccgttctg 1260 gaccagtttc ttcagactcc cgaactgccg gtacgcgaga tttctctgct gagcgatgat 1320 gagaaacacc gcattctcaa cgtttttaac ccgccggtgg cagggctgag cgagggagaa 1380 gcgtttcatc ggtatgttga aaagtttgcc cgggaaattc cggatcatcc ggcagtcgtc 1440 tacatggaca aacagctgac ctacagcgaa ttgaacgaac gcgccgagcg gctggcttct 1500 ctccttcgcg aacagggcgt gggaagggag acgattacgg gtatctgggc ggagcgttcg 1560 gtggaactgc tggtcggggt gctcgccgtt tggaaagccg gcggagccta tgtaccgctg 1620 gaccccgatt atccagcgga gcggattgag tatatgctca gcgatagcga tgcatcggtg 1680 ctgcttacgc agcgtcatct gttggagcgg gccggaggtt ggttggccga tgaccggctg 1740 aagcttcaag ctgtctatgc catggacgat gaacagattt ataacgggga tgccttagcc 1800 gtggaatttg aatctgccga cagcgccccg caagacttgg cttatgtgat ttacacctcg 1860 ggtacgacgg gacgcccgaa aggcgtcatg attgagcacg gtagtctcgt gaatacggcg 1920 gatgcgtacc gtcgcgagta ccggttggat cagtttccgg tgcggctgct gcagctggcc 1980 agcttctcgt ttgacgtgtt tgtcggagac atcgcgcgga cgctatataa cggaggcacg 2040 atggtgattg tgccgaagga tgaccggatt gatccgaacc tcttatacgg ctggattcgg 2100 gaccaaaaca ttacggtatt cgaatcgacg cctgcgctca tcctgccgtt catgcagcat 2160 atttatgaag aagggctgga cgttagctcc atgcagttgc tgattaccag ctcggatgct 2220 tgcagtgtca ccgattaccg attgctgcag gaaagattcg gcggacaatt ccgcatcatc 2280 aacagctatg gcgttaccga agcggccatt gacagcagct tttacggtga gccgctggat 2340 aagttgccgc cgtcgggtca tgtgccgatc ggcaaagctt ggctgaacgc ccggttttac 2400 attgtcgatg ctgcgttaaa gccggttcct gtaggggttc cgggcgagct tgtcatcggc 2460 ggcgccgggg tggcgcgcgg gtactggaac cgtccggatt taaccgccga gaagtttgcg 2520 gacagcccgt ttgtgccggg cgaacgtctg taccggacag gcgatttggc ccgctggcta 2580 gaagacggta acgtcgactt catcggccgg atcgactatc aggtgaaaat tcgcgggttc 2640 cggatcgaac tcggcgaaat tgaaacggcc ctgctgcgtt tcccgggcgt caagcaggct 2700 gtggtgaccg accgtacgga tgagcagggg caaaagtatt tgtgcggcta cgtggtagag 2760 gatgcttccg tgcagctgag cgatctgctg tcccaattga agcaagagct gccggcccat 2820 atggtcccgg cccggctggt gtctcttgat aagcttccgc tcacgccgaa cggcaaaatt 2880 gaccgtaaag cgctgcctga accgaccgga gaggtagaag caggccatga gtatgtggct 2940 cctcgcacaa cgctggaaac aagacttgct ctcatttggc agcaggtgct gggtattgcg 3000 cgagttggag tccaagacga tttctttgac ttgggtggtc attccttgcg ggcctccacg 3060 ctggtttcaa agattcggaa agagctgcaa gtcgaggttc cgctgcggga agttttccgc 3120 tacaccacga tcgaacagct ggcccaaaga atcggcggtt taaggcagca ggagacgtat 3180 gagattacaa aggcggctga ggccgagtac tatccggttt catccgagca aaagcgtctg 3240 tacgtcctgc gccagcttga cggggccgag cgcagctaca atatgtcggc ggcgcttctt 3300 ctcgaaggca agctggaccg cacgcgcgtc gagcacgcgt tccgggcgct gattcagcgc 3360 catgagacgc tgcgtaccgg gattgagcag gttcaaggcg agcttgtcca gcgcatctat 3420 gacgagttgg attttgctgt agattatttc caagcgagcg agcgggaagt ggagcaagtg 3480 gtggaagctt actatcaccc gtttgatctg actaagccgc cacttctccg catcggtctg 3540 atcgaagtcg ccgaggatcg ccacattctg ctgttcgata tgcaccatat cgtctcggac 3600 ggcatttcga cagcgctgct cttcgacgag ttcagccgcc tgtatcgggg cgaggagctg 3660 gccccgctgc gcattcaata caaagattat gccgtttggc agcattccga agcttacggg 3720 cagatgctcc agccgcagaa ggagtattgg ctagaacagc tgtcgggcga gctaccggtc 3780 ttggagctac cgacggactt cccgcgacct gcggtgcaaa gctttgacgg ccgaaccgtg 3840 aagttttata tcgagaaaga tcggacggag aagctgaaag aactagcggc acggacaggg 3900 acgaccctgt acatggtgct gctgtcggct tacaccatcc ttatgcataa atattcgggt 3960 caggaagatc tgatcgtagg aacgccgatt gccggaagaa cgcaggaaga agtgcagccg 4020 atcgtaggga tgtttatcaa cacgctggcc attcgcagcc gtccggagcg ttccaagccg 4080 tacctttcgt acctggaaga aatcaaggac atcacgctcg gggctttcga acaccaaaat 4140 tatttgttcg aagacttggt agaaagtctt cacattccgc gcgcgaccgg ccgtaatccg 4200 ctctttgaca cgttcttctc cctacaaaat acggagaacg agcaaattgt catcgagggg 4260 ctggagcaat cgttttatcc actggaaaat caaacatcca agttcgagct gctcctggat 4320 atttcggagc tggacggtca gctcgaatgc cggttggagt acgctacggc tttgtataaa 4380 caagagaccg cggagcggtt cgccagacat tatgacaagc tgctcgaaac catcgcagca 4440 gcgccggacg gggatattgc ctcgctggaa atgctcacgg aggaggaaat ccgcgagctg 4500 atgcgcggtt tcaacgattc agaggcggac tacccgcggc agcagacgat tcacggcttg 4560 ttcgaagagc aggcggagct ttacccggac aacgtggccg ccgtcatgaa cgagcggcag 4620 ctgacctacc gcgagctgaa cgagcgatcc aaccgccttg cgcggaagct gcgggagacg 4680 ggagtagaag cggaccagct ggtagcgatt ctggccgaac gctcgctcga tatggtcgtc 4740 ggcattctgg cgattctcaa agcgggcgga gcatacgtgc ctgtcgatcc cgactacccg 4800 gaggagtgca tccgcttcat gatcgaggat tcgggtgcgc cgttattgct gattcaaaag 4860 catctgcacg agaagaccga cttcgtagga acgcgcctcg aattggacga tttcgtttgg 4920 ggcgacagag gggcggactc cgaaggtgcg ctggacgctt cgaacctgga gccgatttcc 4980 gggccgctca acctggctta tgtcatctac acgtcgggaa cgaccggcag accaaaagga 5040 acgctgatcg agcataagaa cgtcgtgcgc ctcctgttca atgacaaaaa cctgttcgac 5100 ttcgggccgt ccgacacgtg gacgctgttc cactcgttct gcttcgattt ctccgtctgg 5160 gaaatgtacg gagcgctgct gtacggaggc aagctggtca tcgtaccgcc gttcacggcg 5220 aaaaatccgg ccgatttcct ggcgctgctg ggccgcgaac aggttaccat tttgaaccag 5280 acgccaacgt acttctacca gctgctgcgc gaggtcttga cggaccatcc gtacgatttg 5340 cggattcgca acgtcatctt cgggggcgaa gcgctgagtc cgctgctgct caagggcttc 5400 aagacgaagt acccggagac gaagctgatt aatatgtacg gcattaccga gacgacggtt 5460 cacgttacgt ataaggaaat cacgtgggtc gaaatggagg cggcgaagag caatatcggc 5520 aagccgatcc cgacgctgag ggtgtacgtc cttgatgaaa accgccggcc tgtgccgatc 5580 ggcgtagcgg gcgaaatgta cgtggccggg gaaggccttg cgagaggata cctgaaccgc 5640 ccggatctga cggcggagaa gttcgtcgat tccccgtttg cggaggggga gaaactgtac 5700 cgctcgggcg acttggcggc ttggctgccg gacggcaaca tcgaatacct gggccggatc 5760 gatcaccagg tcaaaatccg cgggtaccgg atcgagctgg acgaaatcga gacgcagctg 5820 ctgaacgtcg agggcgtgga agaagcggtg gtgcttgccc gtcaggatgg cgggggcgag 5880 aaggcgcttg tcgcctactt tgtggcggat cggacactga cggtcagcga aatgagaacc 5940 tcgctggccc agggaatgcc ggggtacatg atcccgtcgt acttcgtgca gctggagcgc 6000 atgccgctga cggccaacgg caaagtagac cgcaaagcgc tgccggagcc gcaaggcagc 6060 ttgcagacgg gcgtcgaata tgtagcaccg cgtaaccgga tggagtccca gcttgtgaag 6120 atctgggagg aagtgctggg ctactccggc attggagtcc tggacaattt cttcgagctc 6180 ggaggccact ccttgcgggc gacgaacctt gtcagcaaga ttcagaagga aatgaacgtc 6240 gaacttccgc tgcgcgatgt gttccgctat acgacggtag agtcgatggc cggggctatt 6300 gccagcttgg aagaaacgcg gcacaactca attccgaaag cggaagagaa agcgtactat 6360 ccggtttcct ccgcgcaaaa aaggctgtac gtcctgcacc agctggatgg ctcggagctg 6420 aattacaacc tcccaagtgc cttgcagttg gaaggggctt tgaacgaagc caaagtggaa 6480 aaggcgctga ctactttggt ggcccggcat gatatgctgc gcaccggttt tgaaattgtg 6540 aatggggagc cggttcagcg tattcatccg atcgtagctt tcaaggtcga aaagcttcaa 6600 gcaagcgaag atcaagttgc ggccattctt gaaggcttca ttcagccttt tgacttgacc 6660 cagccgcctt tgctgcgcgc cctgctgatc gaactggaga aagagaaatt cctgctcgcg 6720 ctggatattc atcatattgg ttccgacggc ctttccatgg acgtgctgct gcgcgaattc 6780 gtgcggcttt acaatgggga agaattgccg gagctgcgga ttcaatacaa ggattacgcc 6840 gtttggcagc aatccgagga gcagcgccag cgcatcaaac ggcaggagga atactggcgt 6900 ggggtattca gctccgagct tccggttctt gagctgcctc tcgacttctc ccgcccggcc 6960 gtccagcagt ttgacggtca aacgctcacg tttacgctgg atgcggagaa aagcgaagct 7020 ctcaaacggc ttgccggcga ttcgggggca acgctgtaca tgcttctgct ggctgcgtac 7080 tccgtattgc ttcataaata cgcgggacag gaagatatag tggtcggcac tccgattgcc 7140 gcccgttctc acgccgacct gcagccgatt atcggtatgt tcgtcaatac gctggccctt 7200 cgcttgggtc cggcggcgga gcggacgttc ctggattact tgcaggaagt gaaggaaaca 7260 acgcttggag cctacgagca ccaagactat ccgttcgagg agctggtgga agctcttcag 7320 gtgagccggg atttaagccg gaatcctctg tttgacacca tgttttcttt gcaaaagcac 7380 gaaagcttgg atttaaccct ggaaggcttg caatggtcgc tgttcgacat cgaggaaaag 7440 acggcaaagt ttgaccttag ctttgatatc gtggaagccg ataacgaact ggtttgcaag 7500 atcgagtacg ctacctcgtt gtttagacag gaaacggtgg tacggctggc aggtcattac 7560 gagcagcttt tgacgtcgat cctggctcag ccgggtgcgc ggatttcgga tttggatgtc 7620 ttgacggaca gcgaaaaaca tgatttgctg gtcgggtttg acgtgtcgtc ttcggctctt 7680 gcgaagcaac ccgccgcaga gggtacaggt ttggaagcgg atgagtcgtg gagagagaga 7740 acgttccacg agctgttcga ggagcaggcg gagcgcactc cggaagcgct ggcggttgtc 7800 tacgaagaca gcaagttgac gtatgcggag ctgaacgcca aagcgaatcg tctggcgtat 7860 gcactgcggg cgcgcggggt gaagccggag caggtggtcg gcattctggc cgatcgttcg 7920 gcggagctgt tgatcggggt gctcgccgta tggaaagcgg gcggcgctta tgtgccgctc 7980 gacccggact atccggcgga gcggatcgag tatatgctcg cggacagcgg ggcgtcggtg 8040 ctgcttacgc agacccgcct gctggagcag gcggaagctt ggcgcagcga cggagctcta 8100 gtgctgcaaa cggtgcttgc gcttgacgac gtcgcgacgt acagcctcgg agcggcggaa 8160 gcggctgtgg gtgtacaagc tttgggcgaa gcaggcgcag aggcggaggc tttggcgcaa 8220 gcggctgctg ccgaaacgtc cgccacggca gaagccgaga agaacgtact agcggcagat 8280 ctcgcatcga atccggcgaa tgtgaacaag ccgcgcgatt tggcttacgt catctacacc 8340 tccggtacga cgggcaagcc gaagggcgtg atgatcgagc accgcagcct ggtgaacaca 8400 gcggcgggct accgtcggga ataccggttg gatcagttcc cggtgcggct gctgcagctc 8460 gcaagcttct cgttcgacgt gttcgtggga gatatcgcgc ggacgctgta caacggaggc 8520 acgatggtaa ttgtgccgaa ggacgaccgg atcgatccgt ctcgtttgca ccactggatg 8580 gagcgggagc gggtcaccat cttcgaatcg acgccagcgc tgatcgtgcc gttcctagag 8640 tatgtgcacg agcagcggct cgatatgagc gggatggagc tgttgatcac gagctcggac 8700 agctgcagcg tagcggatta ccgcaccttg caggagcgct tcggctcgca gttccgtatt 8760 attaacagct acggcgtaac ggaagcggcg attgactcca gcttctatga cgagcctctg 8820 gaaaagctgc cgaagacggg cagcgtgccg atcgggaaag cgtggctgaa cgccaagttc 8880 tacatcgtgg atgcgaatct gaagccggtg ccgatcgggg tgttgggcga gctggttatc 8940 ggcggagtcg gagtggcgcg cgggtacttg aaccgcccgg atttgacggc ggagaaattc 9000 gtagacagcc cgttcgccac tggggagcgg ctgtaccgga caggcgacct ggcgcgctgg 9060 atgtcggacg gcaacgtgga ctttatcggc cggatcgaca accaggtgaa aatccggggg 9120 taccggattg agacaggcga agtcgaagcg aagctgctga gtgtaggtgg cgtgaaggaa 9180 gcggtcgtcg tcgtcaggga agatcaagaa ggtcagaaag ctttgtgcgc ttattataca 9240 gcggaagaag gcttgacagc ggcggatctg aagcgggcga ttgccagcga gctgccgggg 9300 tacatgatcc cgtcgtactt cgtggagctg gagcgcctgc ctctgacgcc gaacggaaag 9360 atcgaccgga aggcgctgcc ggcaccggaa gggggggcag gaggccgcga atacgtggcg 9420 ccgcgcaccg aactggaggc gaagctggcc gccatttggc aggatgtgct tgttcgggag 9480 aaggcggtag gcgtaacgga caacttcttc gacctcggcg ggcactccct gcgggcgacg 9540 acgctggtca gcaaaatgca taaggagcta ggcgttgaat tcccgcttcg cgacgtattc 9600 cgctactcaa cggttgagga aatggccgcg gctatggagc ggctggagat cggctcgttc 9660 acggctattc cggctgcaga atctagcgag tattatccgc tctcttctgc tcagaaacgt 9720 ctctatatct tgaaccagct ggaaggagcc gagctgagct acaacatacc aggagctatg 9780 ctgctcgaag gggagctcga ccggccgcgg tttgaagaag cgttccgcgg gctcgtagct 9840 cgtcatgaaa cgctgcgtac cggctttgag atggtaaaag gcgaagcggt tcagcggatt 9900 tacgaagaag ccgccttcca ggtggaatat gtgcagatca gcgaggagca ggcagaagaa 9960 acggtgcgcc aattcgttcg tccgtttgat ctagcgaagc cgccccttct gcgggtaggc 10020 cttgctgaac tggcgccgga ccggcacatt ctaatgttcg atacgcatca tatcgtatct 10080 gacggcgttt cgattgacgt actgatcgaa gagctcgtcc gcttgtacag cggggagcag 10140 ttggagccgc tccgcattca gtacaaagat tatgcggtat ggcagcaatc ggacgagcag 10200 aaagctcagc ttgccaagca ggaagcctac tggctcgaca tgttccgcgg agaactgccg 10260 gttttggaat tgccgacgga ctatccgcgc ccggctatgc agagctacga gggccgcacg 10320 ctgcaattgt ttatggatag cgagaaaagt gagggtctga aacggcttgc agccgagaac 10380 ggcgcaacgc tttacatggt tctgcttgct ggttatacaa tattattgca taagtataca 10440 gggcaagaag acgtggtggt cggtacgccg attgcaggaa gaaatcacag cgacgttcag 10500 ccgctgatcg ggatgttcgt caataccctg gccatccgca gttatccggc tgctggtaaa 10560 acgttccttg actacttgaa ggaaatcaag gagacgacgc tgggcgcttt tgaacatcag 10620 aattatccgt ttgaggaact ggtggataag gtgaatgtgg ctcgcgattt aagccgcaat 10680 ccgctgttcg atacgatgtt tgctttgcag aatacagaga atttggaaat ccagctcccc 10740 ggactccatt tgtcgacgta tgccagcgaa gaaattgttt ctaaattcga tctcagcttg 10800 gacgtcacag agatcgagga aggcttggaa tatctgtttg aatacgccac tgctctttat 10860 aaaaccgaaa cagtggagaa attggccgct cactacttgc agctgcttga atccattctc 10920 cgcaacccgt cggcgacgat tgccgagctg ggcattttga caccggcgga aaaagagcaa 10980 attcttggcg cgtttaaccc ggcgcagccg gaagcggctc ctgcggcggc gttccaccgg 11040 ctgttcgagg aacaggcgga gcgcacgccg gaagcggcag ctgtcgtgta cgagagcgac 11100 cggctgacgt atgcagagct gaacgagcgg gcgaaccgct tggcggccac ggtgcgcgca 11160 agtggcatcg gccgggatac gatcgtcggc attctcgccg agcgttcggt ggacttgctg 11220 gtggccgtgc tggccgtctg gaaagcgggc ggggcgtatg tgccgctcga cccggattat 11280 ccggcggagc gcgtgcggtt catgctcgaa gacagcggag cgaaggtgct gctgacgcaa 11340 acgctgctgc gagagcgcgc cgaagcctgg ctcggcgaag aggagctggc gctggcggcg 11400 gtgctgtacc tggacgacga agcgtcgtac agcgaggagc gggcgaatgc gccgctggaa 11460 gcggatgatc acttgcacga atgccgtccg gaggacctgg cgtacgtgat ctacacgtcg 11520 ggaacgacgg gcaagccaaa gggcgtgatg atcgagcacc gcagcctggt gaacacggcg 11580 gcgggctacc ggcgggaata ccagttgaat cagttcccgg tgcggctgct gcagctcgcc 11640 agcttctcgt tcgacgtgtt cgtgggagat atcgcgcgga cgctgtacaa cggaggcacg 11700 atggtgattg tgccgaagga cgaccggatc aatccgtctc gtctgcacta ctggatcagc 11760 cagcagcaag tgacgatctt cgaatcgacg ccggcgctga tcgtgccgtt cctggagtac 11820 gtgcacgagc atcaactgga tatgagcggg atggagctgt tgatcacgag ctcggacagc 11880 tgcagcgtgg cggattaccg caccttgcag gaacgcttcg gctcgttgtt ccggatcatc 11940 aacgcatacg gcgtgacgga agcggcgatc gactccagct tctacgacga ggagctggcg 12000 aagctgccac aaacaggcaa tgtgccgatc ggcaaagcgt ggctgaatgc gaaattctac 12060 atcgtggatg cgcatctgaa tccggtgccg gtcggggtgc tgggcgagct ggtcatcggc 12120 ggagtcggag tggcgcgcgg gtacttgaac cgtccggagc tgacggaaga gaagttcgta 12180 gacagcccgt tcgccgcggg cgagcggctg taccgcacgg gagacttggc gcggtggatg 12240 gaagacggga acgtggactt catcggccgg atcgacaacc aggtgaaaat ccgggggtac 12300 cggattgaga caggcgaagt cgaagcgaag ctgctgagtg taggtggcgt gaaggaagcg 12360 gtcgttgtcg tcagggaaga tcaagaaggt cagaaagctt tgtgcgctta ttatacagcg 12420 gaagaagact tgacggcggc ggacctgaag cgggcgattg ccagcgagct gccggggtac 12480 atgatcccgt cgtacttcgt ggagctggag cgcctgcctc tgacgccgaa cggaaagatc 12540 gaccggaagg cgctgccggc accggaaggg ggagcaggcg gaggccgcga atacgtggcg 12600 ccgcgcaccg aactggaggc gaagctggcc gccatttggc aggatgtgct tgttcgggag 12660 aaggcggtag gcgtaacgga taacttcttc gacctcggcg ggcactccct gcgggcgacg 12720 acgctggtca gcaaaatgca taaggagcta ggcgtggaat tcccgcttcg cgacgtattc 12780 cgctactcga cggttgaaga aatggccacg gctatggagc ggctggagat cggctcgttc 12840 acggctattc cggctgcgga acctagcgag tattatccgc tctcttccgc tcagaaacgt 12900 ctctatatct tgaaccagct ggaaggagcc gagctgagct acaacatacc aggagctatg 12960 ctgctcgaag gggagctcga ccggcagcgg tttgaagaag cgttccacgg gctcgtagct 13020 cgtcatgaaa cgctgcgtac cggctttgag atggtaaaag gcgaagcggt tcagcggatt 13080 tacgaagaag ccgccttcca ggtggaatat gtgcagatca gcggggagca ggcggaagaa 13140 acggtgcgcc aattcgttcg tccgtttgat ctagcgaagc cgccccttct gcgggtaggc 13200 cttgctgaac tggcgccgga ccggcacatt ctaatgttcg atacgcatca tattgtatct 13260 gacggcgttt cgattgacgt actgatcgaa gagttcgtcc gcttgtacag cggggagcag 13320 ttggagccgc tccgcattca gtacaaagat tatgcggtat ggcagcaatc ggacgagcag 13380 aaagctcagc ttgccaagca ggaagcctac tggctcgaca tgttccgcgg agaactgccg 13440 gttttggaat tgccgacgga ctatccgcgc ccggctatgc agagctacga gggccgcacg 13500 ctgcaattgt ttatggatag tgagaaaagt gagggtctga aacggcttgc agccgagaac 13560 ggcgcaacgc tttacatggt tctgcttgct ggttatacaa tgttactgca taagtataca 13620 gggcaagaag acgtggtggt cggtacgccg attgcaggaa gaaatcacag cgacgttcag 13680 ccgctgatcg ggatgttcgt caataccctg gccatccgca gttatccggc tgccggtaaa 13740 acgttccttg actacttgaa ggaaatcaag gagacgacgc tgggcgcttt tgaacatcag 13800 aattatccgt ttgaggaact ggtggataag gtgaatgtgg ctcgcgattt aagccgcaat 13860 ccgctgttcg atacgatgtt tgctttgcag aatacagaga atttggaaat ccagctcccc 13920 ggactccatt tgtcgacgta tgccagcgaa gaaattgttt ctaaattcga tctcagcttg 13980 gacgtcacag agatcgagga aggcttggaa tatctgtttg aatacgccac tgctctttat 14040 aaaaccgaaa cagtggaaaa attggccgct cactacttgc agctgcttga atccattctc 14100 cgcaacccgt cggcgacgat tgccgagctg ggcattttga caccgtcgga aaaagagcaa 14160 attcttggcg cgtttaaccc ggcgcagccg gaagcggctc ctgcggcggc gttccaccgg 14220 ctgttcgagg aacaggcgga gcgcacgccg gaagcggcag ctgtcgtgta cgagagcgac 14280 cggctgacgt atgcggagct gaacgagcgg gcgaaccgct tggcggtcac gctgcgcaca 14340 agcggcatcg gccgggatac gatcgtcggc attctcgccg agcgttcggt ggacttgctg 14400 gtggccgtgc tggccgtctg gaaagcgggc ggggcgtatg tgccgctcga cccggattat 14460 ccggcggagc gcgtgcggtt catgctcgaa gacagcggag cgaaggtgct gctgacgcaa 14520 acgctgctgc gagagcgcgc cgaagcctgg ctcggcgaag aggagctggc gctggcggcg 14580 gtgctgtacc tggacgacga agcgtcgtac agcgaggagc gggcgaatgc gccgctggaa 14640 gcggatgatc acttgcacga atgccgtccg gaggacctgg cgtacgtgat ctacacgtcg 14700 ggaacgacgg gcaagccaaa gggcgtgatg atcgagcacc gcagcctggt gaacacggcg 14760 gcgggctacc ggcgggaata ccagttgaat cagttcccgg tgcggctgct gcagctcgcc 14820 agcttctcgt tcgacgtgtt cgtgggagat atcgcgcgga cgctgtacaa cggaggcacg 14880 atggtgattg tgccgaagga cgaccggatc gatccgtctc gtctgcacca ttggattgag 14940 caggagcggg tcaccatctt cgaatcgacg ccggcgctga tcgtgccgtt cctggagtac 15000 gtgcacgaac ataggctgga tatgagctgg atggagctgt tgatcacgag ctcggacagc 15060 tgcagcgtgg cggattaccg caccttgcag gaacgcttcg gctcattgtt ccggatcatc 15120 aacgcttacg gcgtgacgga agcggcgatc gactccagct tctacgacga ggagctggcg 15180 aagctgccgc agacaggcaa tgtgccgatc ggcaaagcgt ggctgaatgc gaaattctac 15240 atcgtggacg cgcatctgaa cccggtgccg gtcggagtgc tgggcgagct ggtcatcggc 15300 ggagtcggag tggcgcgcgg gtacttgaac cgtccggagc tgacagaaga gaagttcgta 15360 gacagcccgt tcgccgcggg cgagcggctg taccgcacgg gagacttggc gcggtggatg 15420 gaagacggca acgtggactt catcggccgg atcgacaacc aggcgaaaat ccgggggtac 15480 cggattgaga cgggcgagat cgagtcgcag ctgctgcggg tggaaggcgt gcgcgaagcg 15540 gtggtgctgg ttcgaagtga tgcgaacggg cagaaagcgc tgtgcgcgta ctacacaacg 15600 gatggggaac tgacggtggc agacctgaaa cgggcgatct ccagcgagct gccgggttac 15660 atgatcccgt cgtacttcgt cgagctggag cgcctgcctc tgacgccgaa cgggaaaatc 15720 gaccggaagg cgctgccggc gccggaaggg ggagcaagcg caggccgcga atacatggcc 15780 ccgcgcaccg aactggaggc gaaactggtc gccatctggc aggatgtgct cggaccggtc 15840 acgattggcg tgacggacaa cttcttcgac ctcggcgggc actccctgcg ggcgacgacg 15900 ctagtcagca aggtgcacaa ggaactgagt gtggacttgc cactgcgcga agtgttccgg 15960 cactcgacca tcgaagcgat ggccgaggcg atcagccaat tggagcggca ggaacacctc 16020 tccattccgg ttctggataa gagggattac tatccgcttt cctccgtgca aaaacggctg 16080 tacatccagc agcagatgga aggcgccgag cttagctaca acatgtccgg catgacggtt 16140 ctcgtcgggc gattggaacg gaatcaattc gaggcggcgc tcaaaggatt gatagctcgt 16200 cacgaaattt tgcgaaccgg cttcgaaatg gtcgacggcg aaccagtaca acggatttat 16260 ccgaacttga agtttgccgt cgaatatacg aaagcgacgg aaagtgaaac gaagagcatc 16320 gtagacggct ttgtgcgcgt ctttgatttg gagcggccgc cgctgctgcg tgtgggctta 16380 gtcgaatggg aagcggaacg gcatctgctc atgctggaca ttcatcatat cgtcacggat 16440 ggcatgtcga tgggcatctt cgtcgaagag ctgctgcgcc tgtataacgg tgagactctg 16500 gagccgcttc ggattcaata caaggaattc gccgcttggc agcagtcgga acctgtaaaa 16560 gagcggctga aacgtcagga agcctactgg ctggacgtgc tggaaggcga actgccgacg 16620 cttgaactgc ctacggactt tgtcagacct gccgctcgca gctttgaggg agatgtgctg 16680 cccttcagca tcgacaagca gatgaccgac agcttgcagc gcatcgccga taagaacggt 16740 gctacccttt atatggtgtt attggcggcc tattcaatcc tgctcagcaa gtactcggga 16800 caagaagact tcattgtagg catgccggtt tcgggccgca cacatgccga cctggagccg 16860 ctcatcggaa tgtttgtcaa cactttggcg attcgccatt atccgtccgg ggagaaggcg 16920 ttcctcgctt acttgaacga agtcaaagaa acgatgctgg gggcctacga ccaccaggat 16980 tatccgttcg aggagcttgt gaaaaagctg caggcgccgc gagatcaaag ccgcaatcct 17040 gtattcgatg tcatgtttgc tctggaaacc aaggaagata acgttcaaag cttcggggat 17100 atcaagatcg aatcttatcc ggaaactcat acggtttccc aatttgatct taccttggtc 17160 atttcgttgc tggatgaggg aatgaacggg cagtttgaat atgccaccaa gttgtttacg 17220 cgcaatctga tcgacaattt cgctcaggac ctgctcatga tcatcaccca aatttgcgaa 17280 cagccttcgg tgctgttgaa ggatatttcc ctgaacgggc aatccgaaca ggagcaagat 17340 gtgctagagg ccattgatat tattttctaa 17370 <210> 15 <211> 1836 <212> DNA <213> Paenibacillus polymyxa ATCC21830 <400> 15 gtgtactgca tggaagctga ccgacagccg tctgttcaag aacaaggcac agcggcgcct 60 ttcaagcgcc aaaccgctta cgccgcctgg aaagcgttcc gctggctgat gtcctatgta 120 agccgtcaca aaggctggat gatcgtcggt gtcttgtccg cgattgccgc cgccgttatt 180 gagatatgga cgggaagttt gatcgagcag ctgaccaccc aggccgagaa gggggcgggg 240 ccaatcgttc tgcaaatcgt gtatgcggtc tttgtggtca tcttgatcgg tgtgccggcg 300 aagtttttca tgagcttcgg cgtggagcga agcagtgcct ctgcggtgca ggatatccgc 360 aaccatgtca tgcgtcatat cggtaaactc ccggtctcct atttagaaaa gcagcactcc 420 ggcgacgtat tgtcgcggat caacaacgac ctgcagctca tccagcagtt tatgattcgg 480 gacctcgccc agtggtttta tcatccgctg ttgttcatcg gctgtttcgc ttatttgctc 540 tacctccaat gggagctgat gttgtacagc ctgctgttat ttcccgtagc gctgctgatt 600 tcccaatgga tcggcaagca gttggagcgg ttgacggagg aagcccaggc gaacatgggc 660 cgaatgaacg tcaacctcca ggatacgctt gggggtatgc ctattgtaaa aagctacctg 720 ctatccggca tgttatctcg ctcctaccaa gtgctgctgc aattgacggc ccaaaaaaag 780 ctggccgtga aaaagcggga agcctgggtc aacccgctgc tttccacgtt gatgatcagc 840 ccgatcattt tcgccgtcag ttacggaagc tatttgatct acaaagggca gctaggcgcg 900 ggagagctga tcgccttcct gtacttgctg aatctgtgtc tggaaccgct ggagcatatt 960 cccgagctca tcacgcggac gttcgaaatg accggtgccc tgagaagggt ctccgaaatc 1020 gtcgagcagc cgaccgaaat ggaaaatggt cgttcgcttc cgaaaacgag cgccgccccc 1080 atcaagtttc agaacgtaac cttcgggtat gaggagagat ccccgatcct gcggaatgtt 1140 agcttctcgg tgccggaagg gaagacgatc gcgcttgtcg gagcgagcgg cggagggaag 1200 agcacggtgt ttaagcttgt atgcggcttt tatccgcttc cgaaggacca gggcgagatc 1260 cgcgtgttcg gcagcctgat ccacggcgcc gatccggagc agcttcggtc gcatttttct 1320 gtggtaaccc aggattcata tttgtttagc ggcacgatcg ctgaaaatat cggctacggg 1380 cgggaagtag cgtcgatgga cgagattatc gaagccgcca aagccgctca ggcgcattcc 1440 ttcattatgc agcttgaggg cggctaccag acgtatgtcg gagagcgcgg aggctttttg 1500 tccggcgggc agcgccagcg cattgcaatg gcccgggctt ttctgaagga tgctcccgtt 1560 ctgctgctgg acgagccaac gtcggctctt gatccggagt cggaaagcgc ggttcaggaa 1620 gcgctgggcg tattgatgaa gcagagaacg acgatgatta ttgcccaccg gctttctaca 1680 gtacaaaacg ccgacgaaat ttgggtcatg gaacagggga gtattgttga aaagggcact 1740 catgaacaat tgctgaagat gaagggactg tacgcccagt cgtactatca ggaatttacc 1800 gagtctgccg aacgcaggga ggtggcgtac acatga 1836 <210> 16 <211> 1734 <212> DNA <213> Paenibacillus polymyxa ATCC21830 <400> 16 atgaaaaagg gcggatggct ctcgcaagtg aaagaactaa gctacttgct gatgtttatg 60 aaccgcaagc gcaaaaccca gtacgccatt ggcctggcag tgacggcgct cacccagaca 120 ttgttcctga tcgccttcag cttggtcgta cataacttgg ttgatttcgc cgtgttccga 180 gatacgtccc tgatagtgga agcctttatt attttgggcg cggccttgtt cctggaaaat 240 gtgatttctc cctggttcat ttacttatac cagcgcagtg tcgagctgac tgtgctgaat 300 atccgcaaac gtctttatga aaagctgtgc cgggtgcggc cgagattcct ggagcagacg 360 caccatgggg acttgctgtc gcgggtgaac aatgacgtaa cgaccgttga gtttacattc 420 tcgcaggttt actttgtttt gctgcttcaa gttgtctttt gcatcggctc cattgtctcc 480 atggtgttga tcgattggcg gtttgccggt gtatccttcg tcattctgct gctgtcctcc 540 gtggtcagcc tgaaatttgc gcgggatatt cgcgccttgt ccgaacaagg cttgcaaacg 600 ctcggtaaaa tgaccgaaaa attcaaagat tttatgggcg gcattcaaat tgtgaagctg 660 ttccgcatcc gcacgattta cggccagtac gaggcgttga acgaacaaat gacgcagacg 720 cttcggcaaa ccgcgcagaa gaacggcatg caggctgcgg tgaatcattt tatcagctac 780 gtcacgttct gcggcatcat cgtcatcggc agtcttcttt atgcctacgg actgatggga 840 atgggaagcg tcgccgccct ggcggttttg caagtgaatc tgacgcacgc cttgttgaac 900 ttggggacgg ttctgtcgat gacccaaaat tcgctcgcgg gcgctcaccg gattcaagaa 960 gtactaagag aggaagagga accggagcgt ttgggatctc ctcacagcga gctcgtgtcg 1020 gaggctgcgg tggagtttcg cgatgtggaa ttttcctatc aggcggataa aaaggtgctt 1080 gtcgacatct ccatgcaggt gtttcccgga caggtcgccg ctatcgtggg ggccagcggc 1140 agcggcaaaa gtacgctgat caagctgctg ctcggctttt atcctgtgga cagcggagaa 1200 atcctgctcc aaggcaaacc gttcggccat tacacgctgg acgaaatccg caggcagatt 1260 gcatacgttc cgcaggagcc gttcttgttt accggcacaa ttgaggaaaa catccgctac 1320 ggcaacctgg atgcaacgga tgaagaagtg atcgaagcgg ccaaagcggc gtacgctcac 1380 catttcattc aggaacttcc tgaacagtat aaaacgccgg tgggagagag aggagcttcg 1440 ttgtcgggcg gacaaaggca gcgaattgcg atcgcacggg ctattctcaa aaatgccccg 1500 attctgctgc tggacgaagc gacttccgcg ctggataacg aatcccagca ttgggtacag 1560 caggccctga acgtattgat gaaagggcgc accaccatcc tgatcgccca ccgtctcagc 1620 accgtggaac atgcggattt gattaccgtc atgaaccaag ggacggtcgt cgagcggggc 1680 tgccatcagg acctgattgc gctcggaggt tattacgccc ggctgtacgg ctaa 1734 <210> 17 <211> 1365 <212> DNA <213> Paenibacillus polymyxa F4 <400> 17 tagcggcgga cgggtgagta acacgtaggc aacctgccca caagacaggg ataactaccg 60 gaaacggtag ctaatacccg atacatcctt ttcctgcatg ggagaaggag gaaagacgga 120 gcaatctgtc acttgtggat gggcctgcgg cgcattagct agttggtggg gtaaaggcct 180 accaaggcga cgatgcgtag ccgacctgag agggtgatcg gccacactgg gactgagaca 240 cggcccagac tcctacggga ggcagcagta gggaatcttc cgcaatgggc gaaagcctga 300 cggagcaacg ccgcgtgagt gatgaaggtt ttcggatcgt aaagctctgt tgccagggaa 360 gaacgtcttg tagagtaact gctacaagag tgacggtacc tgagaagaaa gccccggcta 420 actacgtgcc agcagccgcg gtaatacgta gggggcaagc gttgtccgga attattgggc 480 gtaaagcgcg cgcaggcggc tctttaagtc tggtgtttaa tcccgaggct caacttcggg 540 tcgcactgga aactggggag cttgagtgca gaagaggaga gtggaattcc acgtgtagcg 600 gtgaaatgcg tagagatgtg gaggaacacc agtggcgaag gcgactctct gggctgtaac 660 tgacgctgag gcgcgaaagc gtggggagca aacaggatta gataccctgg tagtccacgc 720 cgtaaacgat gaatgctagg tgttaggggt ttcgataccc ttggtgccga agttaacaca 780 ttaagcattc cgcctgggga gtacggtcgc aagactgaaa ctcaaaggaa ttgacgggga 840 cccgcacaag cagtggagta tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt 900 cttgacatcc ctctgaccgg tctagagata gacctttcct tcgggacaga ggagacaggt 960 ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc 1020 aacccttatg cttagttgcc agcaggtcaa gctgggcact ctaagcagac tgccggtgac 1080 aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc tgggctacac 1140 acgtactaca atggccggta caacgggaag cgaaatcgcg aggtggagcc aatcctagaa 1200 aagccggtct cagttcggat tgtaggctgc aactcgccta catgaagtcg gaattgctag 1260 taatcgcgga tcagcatgcc gcggtgaata cgttcccggg tcttgtacac accgcccgtc 1320 acaccacgag agtttacaac acccgaagtc ggtggggtaa cccgc 1365 <210> 18 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> pmxCC primer <400> 18 gagcttttct gaaggatg 18 <210> 19 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> pmxDN primer <400> 19 ggtcgttacg tcgttgttc 19 <210> 20 <211> 40965 <212> DNA <213> Paenibacillus polymyxa F4 <400> 20 atggcttttg aaaaagaaac gttgttttgg aacgaaaaat tcggtagcga cgattatacc 60 ttgacgcggc tgccttacag caaagctcca agctccctgg cgcccattat gacaaccgtc 120 ggcgggacgc tttcggagga agtggcgcag cgcgtccttc aaatgagcaa gggcgctccg 180 ctggccactt ttatgatttt gctcgctggt gttcagtcgc tcttgcataa atatacaggc 240 gcttctgaca ttctggtcgg catgccggtt gtacggaaac cgacggagac gcgccgatcc 300 gttaatcata cgatcatttt gaaaaactcg ctttcggcgg gtgcgacttt taaaacgctt 360 ctgagcgagc tgaggacttc cttgccggaa gcgattcagc atcaacatat tccgttcttg 420 aaaatgacgg agaagctgga cctgcaatat gcaggcggga tacccgtcgt ccatacgcta 480 gtatccctta aggagctgca tctggacgaa atcgggcaaa acgtggtcac agattgttcc 540 tttgaattca gcttaaccgg cgggacgata cagctagcgc tgtcatataa cgagcactta 600 tatgactccg agttcatgac tcgggtcgtc ggccatctga atcgcctgct ggccgtgggg 660 cttcacgagt tggagctgga catcgtgcgg gcggacatgc tgtcggagga cgagaaattt 720 caattgctgc aaagctttaa cgataccgag aaggactatc cccgtgatcg gacgattcat 780 cagctcgtgg aggagcaggc gaagcgggtg cccgaagcga cggcggttgt ctttgagggg 840 cggcggcttt cgtacgcgga gctgaacgaa cgggcgaacc ggctggcgcg gacgctgcga 900 tcggtcggcg tgctgcccaa tcagctggta ggcatgatgg ccaggagatc gctggagacg 960 gtcgttggca ttctagcggt tttgaaagct ggcggtgcct acgtgccgat cgacccggaa 1020 tacccggaag aacgcatccg ctacattctg gagaactcga acgcgcagct gctgctgact 1080 caaagggagt tgctgcagca ggtgccgttc gaagggaccg tggtggcgct ggatgacgag 1140 caggcctaca gcgacgatgg ctcgaacctg gagccggcca gcggtccgaa cgatctggct 1200 tatgtcatct atacatcagg tacgacgggc aaacccaaag gggtcatgct ggagcatcgc 1260 gggctggtca gcctgaaact gacgtttgcc catacgcttc acacgaccga acaggatcga 1320 gtgctgcaat tcgccagcct atcgttcgat gcgtcgtgct gggagatgtt caacgctctg 1380 tattttgggg cgaccttata tataccgtca accgagacga ttttggatga tcagctgttc 1440 gaacgtttca tgaacgagca tgcaattacg atcgccactt tgccgccaac ttatgcggct 1500 tatttgaatt cagaccgcct gccgagtctg agcaggctca tcacggcggg ctccgccgtt 1560 tcagccgaat tcgtgcagca gtggaaagac aaggtacagt actataatgc gtacggtccg 1620 acggaagcct cgatcgccac atccgtgtgg gcggcgtcta cgcatgacac cgaacgcaga 1680 gcgataccga tcggacgtcc gatctggaac catcgcttgt atatactggg cgcacagaat 1740 cagctggcgc ctatcggagt ggagggagaa ttatgcatcg cgggcgtcgg cctcgcccgg 1800 ggatattgga accggccgga tctgacggcg gagaaattca tcgaacaccc gtttgagccc 1860 ggagagagac tgtaccggac gggcgacttg gcccgctggc tgccggacgg aaatattgag 1920 tacttgggcc ggattgacca tcaggtgaaa atccgtggat tccggatcga gatcggcgag 1980 attgaagagc agcttctgaa gatcgactcc gtgcaggaga cgatcgtaat cgcgcgggaa 2040 ggcaaaagcg ggcaagaact gtgcgcttac ctggtcgcgg ggcgcccgct tacgctcggc 2100 gagctaagaa gcgcgctggc gcaaaaattg ccgaattaca tgattccggc gcattttgtt 2160 cagcttccgc agatgccgct cacgccgaac gacaaaatcg accgcaaggc tttgcccgct 2220 ccggaaggaa acgcgctgac cggcggcgcg tacgtagctc cccgcaatga agccgagcgg 2280 acgcttgccg atgtgtggca ggcggtattg aacgccgatc gcgttggggt aacggatcat 2340 ttcttcgagc tgggcggaga ctcgatcaag tccattcaag tatcttcgcg gcttcatcaa 2400 gccgggtaca agctggaaat ccgggatttg ttcaaatatc cgactatttc acagctcagc 2460 ctgcatgtga aaccgatcgg acgtaccatt gatcaaggcg aaataacggg tgaaacggcg 2520 ctgatgccga ttcagcattg gtttttcgag agctcctttg cggacccgca tcatttcaac 2580 cagtcggtga tgctgtaccg gaaggaacgc ttcgacgaag agacggtgcg tcaggtgctg 2640 caaaagctgg ccgagcatca tgacgccttg cggatggtgt tccgcaagac ggaacaaggg 2700 tttagcgcgc ggaaccgcgc gattcaggaa ggcgggctgt tcacgctgga cgtgttcgac 2760 ttcaaggatg cggagaatac cgcgcaggct gtggaagcga aggcaacgga cattcaagcg 2820 ggcatcgatc tggagaacgg gcccctcgcg aaggcgggac tgttccggtg cgcggacggc 2880 gatcatttgc tgctcgcggt tcatcatgcc gtggtggacg gcgtgtcttg gcgcattttg 2940 atggaggatt tcgctctggg ttacgagcag gccggcaaaa gcgaggaaat tcgtttcccg 3000 gcgaaaacgg atgcgtaccg cacttggtcc gagcagttgg ccgcttacgc gcaaagcccg 3060 gagatggcga aggaacgggc ctattggcag gccgtggaac aaattgcggt tccggccgtg 3120 ccgaaggatc tggaggcgga cgttacgacg cagcaggaca gcgaatcgct gttcgtccgt 3180 ttgactcccg aagaaacgga gctgctgctg aagcgggttc accgggccta caacaccgaa 3240 atgaacgata ttttggtaac ggcgctcggc atagccgttc gcaagtggac gggacacgaa 3300 cgggtgcgga tcaatctcga aggacacgga cgcgaatcga tcggaacgga tatcgacatc 3360 acgcgcacag tcggctggtt tacgaccaag tttccggtcg tcctggagcc ggaaaccgac 3420 cgggatttgg cctatcagat taaacaggtc aaggaaagct tgcgccgcat tccgaacaag 3480 gggcttgggt acggcgtatg ccgctatctc tccaaatcgg aggatggctt tgtttggggc 3540 gcagagccgg aaattaattt taactacctc ggccagttcg gcgatgatgt caatcaggac 3600 gagatcggca tatcttctta ttccagcggc agcccggcca gcgaccggca ggcccgcagc 3660 tttgtgctgg atatcaacgg catggtgctg gacggcgctt tatcgctcga tctcagctac 3720 agccggaagc agtatcgcaa ggaaacgatg gaagccttcg ctcagcggct tgagcaaagt 3780 ctccgagagc tcattaccca ctgcgcaggc aaagaaaaca ccgaattgac gccgagcgac 3840 gtgcaattta aaggcttgac catcgcggaa ttggagcaaa tcgcccagcg ctcgggccat 3900 ctcggggaaa tcgaaaatat ttactcgctt acgccgatgc agaagggcat gtggttccac 3960 agcgcgcttg accggcaaac ggccgcttac ttcgagcaga cgcggtttac gatgcgggga 4020 gcgctcgacg tccagctttt cgagaggagc tggatggagc ttgcgaaacg tcatctggtg 4080 ctgcgggcga attttgtgaa aggaccggcg ggcgagccgc tgcaaatcat ataccgcgac 4140 aagccggtcg gctttgaata tgaagagctg cttcatttgc aggcggacga gaaacaagcg 4200 tatttggata aaaaggcgga ggatgacaag cttcgcggct tcgacatgga acatgacgcg 4260 ctcgttcggg ttacgatcct acgcaccgaa gagcaaagct atcatgtgct gtggagtttc 4320 cagcatattt tgatggacgg ctggtgcctg ccgcagctga cgcaggagct gtttgagacg 4380 tactcggcct tggcatccgg caagcagcca gcgggagata agggatcgga ttatggcgct 4440 tatatcgaat ggttggagaa acaggacgat caggcggcat ccggctattg gacggcattc 4500 ctggcaggtt atgaagggca aaccgtactc ccgggacaaa aggaaccggc gccaaacggc 4560 agatttacgg ctgatcacgt caccgccgag ctgggcaagg acttgagcga gcggatggac 4620 cgggtggcga aacagcgcct ggttacggtc aatacgctgc tgcaagccgc ttggggcgtg 4680 atgctgcaaa aatataacgg aacaaacgat gccgtattcg gcagcgtcgt ggccggaaga 4740 ccggcggaaa tcccgggcat cgagtccatg atcgggctgt tcatcaatac ggtgccggtc 4800 cgcgtcacga gcgaagcgga caccgtgttc gccgacctga tggcgaagct ccaagagcgg 4860 gcgctggagt ccgggcgtta tgattactat ccgctgtatg aaattcaagc ccgcagcgtg 4920 caaaagcaga acctgatcaa ccatatcatc gctttcgaga actatccggt ggacgagcag 4980 atggagcagg cgggcgacca gcagcacggc gacctgacga tcgctgacgt tcagatggag 5040 gagcagacga actataactt caacgtgacc gtggtgccgg gagtcgagat cgaaattcga 5100 ttcgacttta acgccgaagt gttcgataaa gacagcatcg agcggctcaa ggggcatctc 5160 gtccatctgc tggagcaggt gacggataac ccggaaatta ccgtgggcga gctggaactt 5220 gtgacggagg cggaaaaggc cgaccttctc ggacgtttta acgacaccac cacggaattt 5280 ccgcgcggga agacgctcat tcaattgttc gaagagcagg cggagcgcat cccggatgca 5340 gccgctatct ccttgaatga gcaagagctg acctaccgcg agctgaacga acgcgtcaac 5400 cgccttgccc gtaccttgcg tagccacggg atatccaaag gccgtctggt cgccattatg 5460 gctgagcgtt ccatcgaaat ggtggtgggc atgctggcgg cacacaaagc cggagcggct 5520 tacgtaccga ttgacccgga atatcccgag gagcgtatcc gtttcttgat tgaggattcg 5580 ggagcgcagg tcatgctgac gcaaagccgc ttgcgcgagc gcctggcggg ttcggactcc 5640 gtgatcttac tggatgacga gtccttctat cacgaggacg gcacaaatct aaatccgggc 5700 accgaagcga cggatctggc ctgcgtcatc tatacgtcag gcacgacggg caagccgaaa 5760 ggcaaccttg tttcgcaccg caacatcgtg cggatcgtgc gaaatacgaa ttatatcgac 5820 atcaccgaac gggatcatgt cctccagctt tcgagctatt cgttcgacgg agcgattttc 5880 gatattttcg gcgctttgac caacggggcg cggctggtgc tggttccccg cgagactttg 5940 ctggaaatcg tccggctggc ggatctcatc cagcgtgagc gcatctcggt catgctgatt 6000 acgacggctt tcttcaacgt ccttgtagat gtgaacgtcg actgcctgcg ggatgtccgg 6060 gcgattttgt tcggaggaga gcgagtgtcg gtcggacatg tgcgtaaagc gctcgcccat 6120 atcggaccgg gcaggctcaa ccacctgtac ggcccgtcgg aaagcacggt ttataccacg 6180 taccttccgg tcgacttcgt cgatgagtcg gcggttactg tacccatcgg acggccgatc 6240 agtaatacga aggtgtatat cgtcgacagc cggaataagc ttctgccgat cggcgtggcc 6300 ggggagcttt gcgtcggcgg agaaggcttg gtacggggct acaataaccg gccggagctg 6360 acggcggaga aatttgtgga caatccgttt gtgctgggag agcgcatgta ccggacgggg 6420 gatttggcga aatggctgcc ggacggcacg atcgaatacg tgggacggac ggacgaccaa 6480 gtgaaaatcc gcggcttccg tattgagctg ggcgagatcg aagctcagct tcagaaagtg 6540 gagggaattc ggaaagcgac ggtattcgcg agggaagacg cctccggcga gaagcagctt 6600 tgcgcttatt atgaagcgga ccgcgagctt ccggcggccg agctgaagag cgtgctttcc 6660 caggaactgc cggcctatat gattccggcg tacctgatcc agttggagca gctcccgctg 6720 acgacgaacg gcaaggtcga ccgccgatca ctcccggcgc cggaggagag cttgcagccg 6780 ggcgaagaac gtactccgcc tcggactccg ctggaagcca gcttggcggg aatttggaaa 6840 agcgtgctcg gactggagca catcggggtt catgacaact tcttcgacct gggcggccat 6900 tccctgcggg cgacgacact ggtgagcaag gtgcatcagg agctgaacgt cgagctgcct 6960 ctgcgcgacg tattccgcta ctcgacgatc gaagagatgg ctctcgccat ctcccggatc 7020 ggagagcagt cgttctcgtc gattccgctg gcgggcgcaa gagcatatta tccgctttcc 7080 tcagctcaga agcggctgtt tatcctgaat cagctggaag gggccgatca gagctacaac 7140 atgccgggcg tgctgctgct ggaaggatcg attgaccgga gcctgctgga gaaggctttc 7200 cgcggactga tcgcacggca cgaaacgctg cgaaccggct ttgagatcgt acaaggcgaa 7260 gctgtacagc gcatttacga gagcgtcgac tttgccgtcg ggtaccgtca tgtgagcgag 7320 gaagaagcgc ccgaagtcgt gcaggccttc atccggcctt tcgacttggc gaagcctccg 7380 ctgctgcggg cggagctcgt agagctggca gccgaacgtt atttgctgat gttcgacatg 7440 caccatatcg tctccgacgg ggtttcgatg gacgtgttag tcgaggaact cgttcgtctg 7500 tacggcggcg agtcattaga gcctttgcgc attcaataca aggactatgc ggtatggcag 7560 cagtcggacg agcaaaaagt gcagttgaaa cgcgaggaag cttactggct ggaccgttac 7620 cggggcgagc tgccggttct ggaaatgccg acggactatc cgcgtcctgc cgtgcagagc 7680 tttgagggac aaacgctgac gtccttcgtg gacgaggcaa cgaacgaagg cttgaagcag 7740 ctggccgctc aaagaggaac gacgctgtat atggtgctgc ttgcggcata taccgtgctt 7800 ttgcataaat acacaggtca ggacgatttg atcgtcggaa cgtcgattgc gggtagaacg 7860 cacggagaaa cgcagccttt gatcggaatg ttcgtcaata cgctggcgct ccgcaattat 7920 ccggcttcgg agaagacctt cctgtcgtat ctggaagaag tgaaagaaac gaccttaggc 7980 gcttacgagc atcagaatta tccgttcgaa gagctcgtcg ataaagtgca ggtcagccgg 8040 gatttgagcc gcaacccgct gtttgacacg atgttctccc tgcaaaactt ggaggataaa 8100 gagtttgagc tggaagggct gaaattgtcc ccgtacccta gcgaatacgg cacggccaag 8160 ttcgacctga gtgtggatgt tacggaagaa aacggcggcc tggagtgcag ctttgaattc 8220 gcaacggctc tttataaaga aagcacgatc cggcggctgt cgactcattt cggacatttg 8280 ctcgtaacga tcgtaagccg tccggatgcg aagatcgccg agctgaactt gctgacggca 8340 gaggaaaaag aacaaattct cggcgcgttc aacccggcgc agccggaagc ggctcctgcg 8400 tcggcgttcc accggctgtt cgaggaacag gcggagcgca cgccggaagc ggaggccgtc 8460 gtgtacgaga acgaccggct tacgtatgcg gagctgaacg agcgggcgaa ccgcttggcg 8520 gctacgctgc gtgcaagcgg catcggccgg gaggcgatcg tcggcattct cgccgagcgt 8580 tcggtggact tgctggtggc cgtgctggcc gtctggaaag tgggcggggc gtatgtgccg 8640 ctcgacccgg attacccggc ggagcgcgtg cggttcatgc tcgaagacag cggagcgaag 8700 gtgctgctga cgcaaacgcc gctgcgagag cgtgccgaag catggctcgg cgaagaggag 8760 ctggcgctgg cggcggtgct gtacctcgac gacgaagcgt cgtacagcga cgagcgggcg 8820 aatgcgtcgg ttgtctccgg caagctgacg gatgctgtag atgacggcga tgagagccat 8880 cagaatgttg gcaccgacag cttccatgaa gcccgtccgg aggatctggc gtacgtgatc 8940 tatacgtcgg gaacgacggg caagccgaag ggcgtgatga tcgagcaccg cagcctggtg 9000 aatacggcgg cgggctaccg gcgggaatac cggttggatc agttcgcggt gcggctgctg 9060 cagctcgcca gcttctcgtt cgacgtgttc gtgggagata tcgcccggac gctgtacaac 9120 ggaggcacga tggtgattgt gccgaaggac gaccggatcg atccgtctcg tctgcaccac 9180 tggattgagc gggagcgggt caccatcttc gaatcgacgc cggcgctgat cgtgccgttc 9240 ctagagtacg tgtacgagca gcaactggat atgagccggt tggagctgtt gatcacgagc 9300 tcggacagct gcagcgtggc ggattaccgg accttgcagg aacgcttcgg ctcgttgttc 9360 cggatcatca acgcatacgg cgtgacggaa gcggcgatcg actcaagctt ctacgacgag 9420 gagctggcga agctgccgca gacaggcaat gtgccgatcg gcaaagcgtg gctgaatgcg 9480 aaattctaca tcgtggatgc gcatctgaac ccggtgccgg tcggggtgct gggcgagctg 9540 gtcatcggcg gagtcggagt ggcgcgcggg tacttgaacc gtccggagct gacagaagag 9600 aagttcgtag acagcccgtt cgccgagggc gagcggctgt accgcacggg agacttggcg 9660 cggtggatgg aagacggcaa cgtggacttc atcggccgga tcgacaacca ggcgaaaatc 9720 cggggctacc ggatcgagac gggcgagatc gagtcgcagc tgctgcgggt ggaaggcgtg 9780 cgcgaagcgg tggtgctggt tcgaagtgac gcgaacgggc agaaggcgct atgcgcgtat 9840 tacacgccgg ataccggagc ggaactggcg gtgaacgatc tgcgcggcgc gctggcgcag 9900 gagctgccgg gctacatgat cccgtcgtac ttcgtggagc tggagcgcct gcctctgacg 9960 ccgaacggaa agattgaccg gaaggcgctg ccagcgccgg aaggggaagc gggaagcgga 10020 acggagtacg tcgcaccgcg caatgagctg gaaacgaagc tggcggcgat ttggcaggag 10080 gtgctggggc ttgcgaagga gattggcgtt cacgacaact tcttcgacat cggcggccac 10140 tccctgcggg cgacgacgct ggtcagcaag attcataaag agttgaacgt ggatctgccg 10200 ctgcgcgacg tgttccgcca ttccacgatc gagagcatgg cggccgccat ttcccggctg 10260 gatgagcaaa cattcgttgc cattccggtg gcggatgacc gagaggtgta cccgcaatct 10320 tttgctcaaa aacgtctctt tatcctgaat caactggaag gcgcggagct tagctacaac 10380 atgccggaag cgatgctgct ggagggtgct ttagaccggg caaggttcga agaagcgttc 10440 cgtaagctcg tggcgcggca tgaaacactg cgcaccgggt tcgaaatggt ggatggcgaa 10500 gcatcgcagc ggatttacca ggacgtgaat tttgccgtgg agttctatcg agcggatgag 10560 caagaggccg aagagacggt tcgccgtttc gtccgtccgt ttgacttggc gaagcctccg 10620 ctgctgaggg taggccttgt cgagctggct ccggaacgcc atattctaat gtacgacatg 10680 catcatatta tttccgacgg cgtctcgatg gaaatctttg ttgaagaatt cgtccgcttg 10740 tacggcggcg agcaattgga gcctctgcgc attcagtaca aagactacac cgtttggcag 10800 cattcgcagg agcagaagga acggcttcag cgtcaggagg cgtactggct ggacatgttc 10860 caaggcgagc ttccggtgct ggaaatgccg accgactatc cgcgtccggc cgtgcagagc 10920 tacgaaggcc aaacgctgga gtttttcttt gacgcttcga aaaccgacgg cctgaagcag 10980 ctggcctcgg aaacgggcac gacgctgttt atggtgctgc ttgcggcgta taacgtcctt 11040 ctgcataaat attcaggtca ggaagacgtg atcgtcggta cgccgattgc cggaaggaat 11100 catggagatg tgcagccgtt gatcgggatt ttcttaaaca cgctggctat ccgcagttat 11160 ccggcttcgg agaagacatt cctgtcatac ctgaacgaag tcaaagaaac gaccctccac 11220 gccttcgagc atcaaaacta tccgttcgaa gaattggtgg acaaggtgca agtcacccgt 11280 gatttaagcc gcaatccgct cttcgacacg ctgtttacga tgcagaatac ggagaacgag 11340 gaatttgagc tggaagggct tcgcctgatt ccttatccga gcgcactgga taccgcgaag 11400 tttgatatca gcttggatgt gggcgaggag aacggcggct tggattacag cttcgaatat 11460 gcgacggctc tctacaaaag ggagacgatc gaacggctgg caaaacatta cgagcagctg 11520 ctcgtaacga tcgtaagccg tccggatgcg aagatcgccg agctgaactt gctgacggca 11580 gaggaaaaag aacaaattct cggcgcgttc aacccggcgc agccggaagc ggctcctgcg 11640 gccgcgttcc accggctgtt cgaggaacag gcggagcgca cgccggaagc ggaggccgtc 11700 gtgtacgaga acgaccggct gacgtatgcg gagctgaacg agcgggcgaa ccgcttggcg 11760 gctacgctgc gtgcaagcgg catcggccgg gagacgatcg tcggcattct cgccgagcgt 11820 tcggtggact tgctggtggc cgtgctggcc gtctggaaag cgggcggggc gtatgtgccg 11880 ctcgacccgg attacccggc ggagcgcgtg cggttcatgc tcgaagacag cggagcgaag 11940 gtgctgctga cgcaaacgcc gctgcgagag cgtgccgaag cctggctcgg cgaagaggag 12000 ctggcactgg cggcggtgct gtacctggac gacgaagcgt cgtacagcga ggagcgagcg 12060 aatgcgtcgg ttgtctccgg caagctgacg gatgctgtgg acgacggcga tgagagccat 12120 cagaatgttg gcaccgacag cttccatgaa gcccgtccgg aggatctggc gtacgtgatc 12180 tatacgtcgg gaacgacggg caagccgaag ggcgtgatga tcgagcaccg cagcctggtg 12240 aacacggcgg cgggctaccg gcgggaatac cggttggatc agttcccggt gcggctgctg 12300 cagctcgcaa gcttctcgtt cgacgtgttc gtgggagata tcgcccggac gctgtataac 12360 ggaggcacga tggtgattgt gccgaaggac gaccggatcg atccgtctcg tctgcaccac 12420 tggattgagc gggagcgggt caccatcttc gaatcgacgc cggcgctgat cgtgccgttc 12480 ctggattacg tgcacgagca gcaactggac atgagctgga tggagctgtt gatcacgagc 12540 tcggacagct gcagcgtggc ggattaccgg accttgcagg aacgcttcgg ctcgttgttc 12600 cggatcatca acgcatacgg cgtgacggaa gcggcgatcg actcaagctt ctacgacgag 12660 ccgctgacga agctaccgca gacaggcaat gtgccgatcg gcaaagcgtg gctgaatgcg 12720 aaattctaca tcgtggatgc gcatctgaac ccggtgccgg tcggggtgct gggcgagctg 12780 gtcatcggcg gagtcggagt ggcgcgcggg tacttgaacc gtccggagct gacggaagag 12840 aagttcgtag acagcccgtt cgccgcgggc gaacggctgt accgcacagg agacttggcg 12900 cggtggatgg aggacggcaa cgtggacttc atcggccgga tcgacaacca gacgaaaatc 12960 cgggggtacc ggatcgagac gggcgagatc gagtcgcagc tgctgcgggt ggaaggcgtg 13020 cgcgaagcgg tggtgctggt tcgaagtgac gcgaacgggc agaaggcgct atgcgcgtat 13080 tacacgccgg ataccggagc ggagctggcg gtgaacgatt tgcgcagcgc gctggcgcag 13140 gagctgccgg gctacatgat cccgtcgcac ttcgtggaga cggaacgcct gcctctgacg 13200 ccgaacggaa agattgaccg gaaggcgctg ccagcgccgg aaggggaagc gggaagcgga 13260 acggagtacg tcgcaccgcg caatgagctg gaaacgaagc tggcggcgat ttggcaggag 13320 gtgctggggc ttgcgaagga gattggcgtt cacgacaact tcttcgacat cggcggtcac 13380 tccctgcgag cgacgacgct ggcgggcaag gtatttaagg aattaaacgt caacctgccg 13440 ctgcgcgacg tattccgtca ctcgacgatt gcggcgatgg ccgaggcgat cgcccggatg 13500 gaacggcggg agcatgaggc cattcctcaa gcggaggaga gagagtacta ccctctgtcc 13560 tccgcgcaga aacggctgtt cattcagcac acgctggatg gagcggatca gctttacaac 13620 atgccggaac tggtgcaggt ggaaggcgag tttgatttag accggttgga agccgccttg 13680 cggaaattga taacacggca tgaatcgctg cgcaccggtt ttgaaatcgt gaaggccgaa 13740 gcggttcagc ggatttaccc gcaggtcgat tttgctatcg agcatcatca agcggataaa 13800 gaggatgcgg ctcaaatcga gcagatcgtc cgcagcttcg ttcgtccgtt tgatctcggc 13860 aagccgccgc tgctgcgcgc cggggtcatc gagctggagc cgaacctgca tattctcctt 13920 ttcgacatgc accatatggt gtccgacggc gtatcgatgg cgattgtgat cgatgagttc 13980 tcgagtttct acgccgggga agaactgccg ccactgcgca ttcaatacaa ggattatgcc 14040 gtttggcagc agtcgaaggc ccaccgagag cggatcgggc ggcaggaagc gtactggctg 14100 caaaccttcg aaggcgagct gccgacggcg gacctgccga tggactacga acggtccgcg 14160 gctcgcagct acgaaggcgc gcatctggag ttcgacgtcg aagcttctct ctctgcgcgg 14220 ctgcgcgaat tggcggccga gcgtgaaagc acgctgttca tggtgctgct tgcggcttat 14280 accgtgctgc tgtccaagta cagcgggcag gaggacttgg tcgtgggcac cccggtggcg 14340 ggaagaacga acgccgattt ggaaccggtc atcgggatgt ttgtcaatac gctggcgatc 14400 cgcaatcgtc cgtcgggcga caaaacgttc ttgtcctacc tggaagaagt gaaggaaacg 14460 gctttgggtg ctttcgagaa ccaggattat ccattcgagg agctcgtgga gcgtttgaat 14520 gtgaagcggg agccgggccg cttcccgctg ttcgatgccg ttttcgactt gcaaaatatc 14580 gaagaacgag acgccgagct ggaaggggtc agcctgaaga cttacgagct tgaccatttg 14640 gaagaagcga agttcgatct gacgctgttt atgtatgaaa acaacggggc gctgagcggg 14700 ggcttcttct acgccaccaa gcttttcaaa gaagcgatga tccgcacctt gaccgaggat 14760 tacctgcggg tactgtctca aattgcggaa aatccacaac tcgagctaag ccggattgaa 14820 tgtcataaac cggcggcagg cgcaaagagt gccgtcgata cgatcgaatt cgcgttctaa 14880 tcctacaagc gcgcctccgc cgtcaagtga aagcggcgcg catcccaagg aagacggcga 14940 acaacgtcca agcccgtaaa cgcgcaggcc gaaagcaagc tttttcggac ctgcgccggg 15000 gcatgcggtc acttcatttt taggggaggt acaaatgaaa tctttgtttg aaaaggaaga 15060 acggtactgg agcggcaagt ttgacgccga tgacagcctg agcttccttc cctacagtca 15120 atcctccaaa ttatccgccg acggggaagc tgcggccgag ccgggcttgc ttcaccgtac 15180 tctgccgagc gaactctcgg agagaatcat ttgcctcgcc aacggttcgg atttggcttt 15240 gtacatgatt gttttggcag gagtaaaaag cctgctgttc aagtataccg ggcgggacca 15300 agtgctggtc ggcatgcctt cttatagcgc agaccccgac gggactccgc cgccgcatga 15360 catcttggtg atcaagacgt ccgtaagccg ccagactacg ctgaaaacgc tgctcggggg 15420 catcaaagcc tccatcggcg aggcgctgga gcatcagcac ctgccttttc ggaaaatggt 15480 ggagccgctc catctggact atacggggga cggcctcccg gtcgtcaaca ccgtcgtatc 15540 cttcgccccg attcatccgg aaccgctggg taaccgggtg gcggccgata cggtttttcg 15600 cttcgaccgc caaaaccact ccatcgagct ggaaataagc tttgacgggc agcgttacga 15660 gcgggcattt gtggaacagg cggccgacca tcttgttagg ctgctgtccg tgcttttatt 15720 tcagccggat ctggagcttg gacaagccga tgtgctgtcc ccagacgaga gggagacgct 15780 gctgaagcga tttaatgaca ccgaaaccga gttcgagcgg gggaaaacga tttacggctt 15840 gttcgaagaa caggcggagc tttacccgga caacgtggcc gccgtcatga acgagcggca 15900 gctgacctac cgcgagctga acgagcgatc caaccgcctt gcgcggaagc tgcgagagac 15960 gggagtagaa gcggaccagc tggtagcgat tctggccgaa cgctcgctcg atatggttgt 16020 cggcattctg gcgattctca aagcgggcgg agcatacgtg cctgtcgatc ccgactaccc 16080 ggaggagcgc atccgcttca tgatcgagga ttcgggcgcg ccgttattgc tgattcaaaa 16140 gcatctgcac gagaagaccg acttcgcagg aacgcgcctt gaattggacg atttcgtgtg 16200 gggcgacaga ggggcggact ccgccgatgt gcgggacgct tcgaacctgg agccgatttc 16260 cgggccgggc aacctggctt atgtcatcta cacgtcggga acgaccggca gaccgaaagg 16320 aacgctgatc gagcataaga acgtcgtgcg cctcctgttc aacgacaaga acctgttcga 16380 cttcgggccg tccgacacgt ggacgctgtt ccactcgttc tgcttcgatt tctccgtctg 16440 ggaaatgtac ggagcgctgc tgtacggagg caagctggtc atcgtaccgc cgctcacggc 16500 gaaaaatccg gccgatttcc tggcgctgct gggccgcgaa caggtcacga ttttgaacca 16560 gacgccaacg tacttctacc agctgctgcg taaggtcttg gcggaccatc cgtacgatct 16620 gcggattcgc aacgtcatct tcgggggcga agcgctgagt ccgctgctgc tcaagggatt 16680 caagacgaag tacccggaga cgaagctgat caatatgtac ggcattaccg agacgacggt 16740 tcacgttacg tataaggaaa taacgtgggt cgaaatggag gcggcgaaga gcaatatcgg 16800 caagccgatc ccgacgctga gcgtgtacgt ccttgatgaa aaccgctccc ctgtgccgat 16860 cggtgtagcg ggcgaaatgt acgtggccgg ggaaggcctt gcgagaggat acctgaaccg 16920 tccggatctg acggcggaga agttcgtcga ttccccgttt gcggaggggg agaaactgta 16980 ccgctcgggc gacttggcgg cttggctgcc ggacggcaac atcgaatacc tgggccggat 17040 cgatcatcag gtgaaaatcc gtgggtaccg gatcgagctg gacgaaatcg agacgcagct 17100 gctgaagatc gccgccgtgc aagaagccaa ggtgctcgac cgcgacgacg cgaacggcca 17160 taagcagctt gtcgcttatt acgtcgcgga aacgaggctg gcggcgcatg aactcaagga 17220 ggagctcgcc aagcagcttc cggggtatat gattccttcg cacctcgtgc agctttcgcg 17280 gatgccgctg accccgaacg ggaaaatcga ccgcaaagcg ctgcccgcgc cggaggaagc 17340 cgcggccgga ggagcggaat atgtcgcgcc gagaacgctg ctcgaaatga agatcgcccg 17400 cgtctggcag gatacgcttg gcgttccgca ggtcggcgta aaggataact tttttgagtt 17460 gggtggcaat tcgttaagtc tgatgaggct cgttcaagcc gtttacgatg aaacggacat 17520 cgagataccg ctgaaccgcc aattccacaa tctaacagtt gaagccatgg ctttcggcga 17580 gggggatctg ggcctggata aagggggaga ctccttcatt aagctgaata aagcaggaga 17640 tctgaacgtg ttctgcttcc ctccgggcag cggcttcggc atcggttacc gagagctcgc 17700 aagcaggctc gacggccggt tcgtgctcta cggcattgat tttatcgacg atgccgtcga 17760 ttacgaggcc atgctgaacc gttatgtgga cgagatcgtc cgcatccagc cggaaggacc 17820 ttacgtgctg ctcggctact gcttcggagg caacctgacg ttcgaggtag ccaaaacgat 17880 ggagaaaaga gggtattccg taacggacgt gctcatgttg gactcgtgga ttaaggacac 17940 gctgacgcct tccgaaacgt cggagaaaga gcttgaagaa acgcttgccg atttcgacga 18000 agaagagaag gaattaatga gcaacccgct cgtgcgggag cgagttcatc ggaaggtcaa 18060 agcgaccttg gcgtacgaag cgcagcttat taattccggc acgatcccgg ccaggattta 18120 cgaactgatt gcgaaggaca gcgaagcgtt ccgcctggag caccaattgc cttcctggcg 18180 gggggcaacg acgcaagctt acgccgatta ccggctggag ggcgcgcacg aggaattgct 18240 ggaactcgcg cgcgtggaag aaacggccgt cgtcatccgg gacatcttag agcaagtcaa 18300 gcggcggatc gaagcggagg ccggggtact gcatggaagc tgaccgccag ccgtctgttc 18360 tagaacaagg cacagcggcg ccttccaagc gccaaaccgc ttacgccgcc tggaaagcgt 18420 tccgctggct gatgtcctat gtaagccgtc acaaaggctg gatgatcgtc ggtatcttgt 18480 ccgcgattgc cgccgccgtt attgagatat ggacgggaag tttgatcgag cagctgacca 18540 cccaggccga gaagggggcg gggccaatcg ttctgcaaat cgtgtacacg gtctttgtgg 18600 tcatcttgat cggtgtgccg gcgaagtttt tcatgagctt cggcgtggag cgaagcagcg 18660 cctctgcggt gcaggatatc cgcaaccatg tcatgcgtca tatcggcaaa ctcccggtct 18720 cctatttgga aaagcagcac tccggcgacg tattgtcgcg gatcaacaac gacctgcagc 18780 tcatccagca gtttatgatt cgggacctcg cccagtggtt ttatcatccg ctgttattca 18840 tcggctgttt cgcttatttg atctacctcc aatgggagct gatgctgtac agcctgctgt 18900 tatttcccgt agcgctgctg gtttcccaat ggatcggcaa gcagttggag cggttgacgg 18960 aggaagccca ggcgaacatg ggccgaatga acgtcaacct ccaggatacg cttgggggta 19020 tgcctattgt aaaaagctac ctgctatccg gcatgttatc tcgctcctac caagtgctgc 19080 tgcaattgac ggcccaaaaa aagctggccg tgaaaaagcg ggaagcctgg gtcaacccgc 19140 tgctttccac gctgatgatc agcccgatca ttttcgccgt cagttacgga agctatttga 19200 tctacaaagg gcagctaggc gcgggagagc tgatcgcctt cctgtacttg ctgaatctgt 19260 gtctggagcc gctggagcat attcccgagc tcatcacgcg gacgttcgaa atggccggtg 19320 ccctgagaag ggtctccgaa atcgtcgagc agccgaccga aacggaaaat ggccgttcgc 19380 ttccgaaagc gagcgccgcc cccatcgagt ttcagaacgt aaccttcggg tatgaggaga 19440 gctccccgat cctgcggaat gttagcttct cggtgccgga agggaagacg atcgcgcttg 19500 tcggagcgag cggcggaggg aagagcacgg tgtttaagct tgtatgcggc ttttatccgc 19560 ttccggagga ccagggcgag atccgcgtgt tcggcagcct gatccacggc gccgatccgg 19620 agcagcttcg gtcgcatttt tccgtggtaa cccaggattc atatttgttt agcggcacga 19680 tcgctgaaaa tatcggctac gggcgggaag tagcgtcgat ggacgagatt atcgaagccg 19740 ccaaagccgc tcaggcgcat tccttcatta tgcagcttcc cgacggctac cagacgtatg 19800 tcggagagcg cggaggcttt ttgtccggcg ggcagcgcca gcgcattgca atggcccggg 19860 cttttctgaa ggatgctccc gttctgctgc tggacgagcc aacgtcggct cttgatccgg 19920 agtcggaaag cgcggttcag gaagcgctgg gcgtattgat gaagcagaga acgacgatgg 19980 ttattgccca ccggctttct acagtacaaa acgccgacga aatttgggtc atggaacagg 20040 ggagtattgt tgaaaagggc actcatgagc aattgctgaa gatgaaggga ctgtacgccc 20100 agtcatacta tcaggaattt accgagtctg ccgaacgcag ggaggtggcg tacacatgaa 20160 aaagggcgga tggctctcgc aagtgaaaga actcggctac ttgctgatgt ttatgaaccg 20220 caagcgcaaa acccagtacg ccattggcct ggccgtgacg gcgctcaccc agacattgtt 20280 cctgatcgcc ttcagcttgg tcgtacataa cttggttgat ttcgccgtgt cccgagatac 20340 gtccctgatg gtggaagcct ttattatttt gggcgcggcc ttgttcctgg aaaatgtgat 20400 ttctccctgg ttcatttact tataccagcg cagtgtcgag ctgactgtgc tgaatatccg 20460 cgaacgtctt tatgacaagc tgtgccgggt gcgaccgaga ttcctggagc agacgcacca 20520 tggggacttg ctgtcgcggg tgaacaacga cgtaacgacc gttgagttta cattctcgca 20580 ggtttacttc gttttgctgc ttcaagttgt cttttgcatc ggctccattg tctccatggt 20640 gttgatcgat tggcggtttg ccggcgtatc cttcgtcatt ctgctgctgt cctccgtggt 20700 cagcctgaaa tttgcgcggg atattcgcgc cttgtccgaa caaggcttgc aaacgctcgg 20760 taaaatgacc gaaaaattca aagattttat gggcggcatt caaattgtga agctgttccg 20820 catccgcacg atttacggcc agtacgaggc gttgaacgaa caaatgacgc agacgcttcg 20880 gcaaaccgcg cagaagaacg gcacgcaggc tgcggtgaat cattttatca gctacgtcac 20940 gttctgcggc atcatcgtca tcggcagtct tctttatgcc tacggactga tgggaatggg 21000 aagcgtcgcc gccctggcgg ttctgcaagt gaatctgacg cacgccttgt tgaacttggg 21060 gatggttctg tcgatgaccc aaaattcgct cgcgggcgct caccggattc aagaggtact 21120 aagagaggaa gaggaaccgg agcgtttggg atctcctcac agcgagctcg tgtcggaggc 21180 tgcggtggag tttcgcgatg tggtattttc ctatcaggcg gataaaaagg tgcttatcga 21240 catctccatg caggtgtttc ccggccaggt cgccgctatc gtgggggcca gcggcagcgg 21300 caaaagtacg ctgatcaagc tgctgctcgg cttttatcct gtggacagcg gagaaatcct 21360 gctccaaggc aaaccgttcg gccattacac gctggacgaa atccgcaggc agattgcata 21420 cgttccgcag gagccgttct tgtttaccgg cacgattgag gaaaacatcc gctacggcaa 21480 cccggatgca acggatgaag aagtgatcga agcggccaaa gcggcgtacg ctcaccattt 21540 cattcaggaa cttcctgaac agtataaaac gccggtggga gagagaggag cgtcgttgtc 21600 gggcggacaa aggcagcgaa ttgcgatcgc ccgggcgatt ctcaaaaacg ccccgattct 21660 gctgctggac gaagcgactt ccgcgctgga taacgaatcc cagcattggg tacagcaggc 21720 cctgaacgaa ttgatgaagg ggcgcaccac cattctgatc gcccaccgtc tcagcaccgt 21780 ggaacatgcg gatttgatta ccgtcatgaa ccaagggacg gtcgtcgagc ggggccgcca 21840 tcaggacctg ctggcgctcg gggggtatta cgcccggctg tacggctaga cccgctttac 21900 ggcgggcggc ttcctgcttg cgaatgcgac agagtctttt agtaatccaa ggtatgactt 21960 tataaccggt aattaccaga aaacgtagac acgaaaaagg aggaagagtc cgaaggagcc 22020 gttccgggaa tggcctgcac ggttcttcta agagtgacta agtgacatct aggtacaaag 22080 cctatctata aagaatctct aaaccgggag tgtgtcgatg tgagagagaa taccaacgag 22140 caatatggat taacgcaagc ccagcgccga atatggttca tggaaattat gaatccggga 22200 acgtccatca cgatgctttc cgcgacctac cagattacgg gcgagatcaa cacacagctt 22260 ctggagcaag cggcggcaga gatcgtcaaa acctatgacg ttttccgaat ccgcattagc 22320 ggggatttgc aaaatccaac gcagtggttc gaagaaccgg agaatgtcca agctaggata 22380 agccgcctcg aaataggcac aaccgaacaa ttctatgctt gggtgaaaga agtaagcgaa 22440 aaaccggcca gcgtgttcga cgaacacctc taccaattta cgattatcca ttttgcgaac 22500 ggccaagtat ggctcaattt gacggtaaat catattatcg ccgacggctt gtccgtcaat 22560 gctttgctgc atgcggtgat ggaaaaatac ctggaactgc gcaaaggcat ctccagcagt 22620 taccaggccc cttcctatct ggattatatt tctgcggagc gtgaatatga gcaatcgcag 22680 cgttatcaaa aaggcaagga atactggctg acgaagtaca acactttgcc tgaaacgacc 22740 ggcattaaat cgtatccgcc attctcgatc ggcagcgaat ccaataaact ggccatcact 22800 ttggacggtt cccggtatga acgcattctg gccttcagcg aacaatatca ggtcagctta 22860 tatacgttat ttctgtccgc catgtacgcc ttattgtaca agctgaccga cagcaccgat 22920 gttccggtcg gcacggtttt cgccaatcgc accagcaaga aggaaaaaga aacgatcggc 22980 atgttcgtca gcaccgtggc tacgcggatc cgtctgaatc cagacaggca cgtgctttcc 23040 ttgatccaaa cggtttccaa ggaaaatacg gcggatctgc ggtatcagaa atacccttat 23100 aaccaattga tccaggattt acgcgaacaa cacggccgca acgatctttc ggggctgttc 23160 cgcacgtctc tggaatatct gcctttgaaa atcgtggagt acgaagaaat caaggtacgc 23220 ctggaggctc acttcgctag gcacgagatg gacgatttgc tgctgcgctt cgaccatatg 23280 ctgaatgaag gccatgtaat tctccatgct tcctatcgta ccggcctgtt cgagacggcc 23340 gagattgatc ggattatgga acagtatgta accgttctgg accagtttct tcagactccc 23400 gaactgccgg tacgcgagat ttctctgctg agcgatgagg agagacaccg cattctcaac 23460 gtttttaacc cgccggtggc agggctgagc gagggagaag cgtttcatcg gtatgttgaa 23520 aagtttgccc tggaaattcc tgatcatccg gcagtcgtct acatggacaa acagctgacc 23580 tacggcgaat tgaacgaacg cgccgagcgg ctggcttctc tccttcgcga acagggcgtg 23640 ggaagggaga cgattacggg gatctgggcg gagcgttcgg tggaactgct ggtcggggtg 23700 ctcgccgttt ggaaagccgg cggagcctat gtaccgctgg accccgatta tccggcggag 23760 cggattgagt acatgctcag cgatagcgaa gcaacggtgc tgcttacgca gcgtcatctg 23820 ttggagcggg ccggagtttg gttggccgat gaccggctga agcttcaagc tgtctatgcc 23880 atggacgatg aacagattta taacggggat gccttagccg tggaatttga atctgccggc 23940 agcgccccgc aagacttggc ttatgtgatt tacacctcgg gtacgacggg acgcccgaaa 24000 ggcgtcatga tcgagcacgg tagtctcgtg aatacggcgg atgcgtaccg tcgcgagtac 24060 cggttggatc agtttccggt gcggctgctg cagctggcca gcttctcgtt tgacgtgttc 24120 gtcggagaca tcgcgcggac gctgtataac ggaggcacga tggtgattgt gccgaaggat 24180 gaccggattg atccgaacct cttatacggc tggattcggg accaaaatat tacggtattc 24240 gaatcgacgc ctgcgctcat cctgccgttc atgcagcata tttatgaaga agggctggac 24300 gtcagctcca tacagttgct gattaccagc tcggatgctt gcagtgtcac cgattaccga 24360 ttgctgctgg aaagattcgg cggacaattc cgcatcatta acagctatgg cgttaccgaa 24420 gcggccattg acagcagctt ttacggtgag ccgctggata agctgccgcc gtcgggtcat 24480 gtgccgatcg gcaaagcttg gctgaacgcc cggttttaca ttgtcgatgc cgcgttaaag 24540 ccagttcctg taggggttcc gggcgagctt gtcataggcg gcgccggggt ggcgcgcggg 24600 tactggaacc gtctggacct aaccgccgag aagtttgtgg acagcccgtt tgtgccgggc 24660 gaacgtgtgt accggacagg cgatttggcc cgctggttgg aagacggcaa cgtcgacttc 24720 atcggccgga tcgactatca ggtgaaaatt cgcgggttcc gtatcgaact tggcgaaatt 24780 gaaacggccc tgctgcgttt cccgggcgtc aagcaggctg tggtgaccga ccgcacggat 24840 gagcaggggc aaaagtattt gtgcggctac gtggcggcgg atgcttcctt gcagctgagc 24900 gatctgctgt cccaattgaa gcaagagctg ccggcccata tggtcccggc ccggctggtg 24960 tctcttgata agcttccgct cacgccgaac ggcaaaattg accgtaaagc gctgcctgaa 25020 ccgaccggag aggtagaaac aggcggtgag tatgtggctc ctcgcacaac gctggaaaca 25080 agacttgctc tcatttggca gcaggtgctg ggtattgcgc gagttggagt ccaagacgat 25140 ttctttgact tgggtggtca ttccttgcgg gcctccacgc tggtttccaa gattcggaaa 25200 gagctgcaag tcgaggttcc gctgcgggaa gttttccgct acaccacgat cgaacagctg 25260 gcccaaagaa tcggcggttt aaggcagcag gagacgtatg agattacaaa ggcggctgag 25320 gccgagtact atccggtttc atccgagcaa aagcgtctgt acgtcctgcg ccagcttgac 25380 ggggccgagc gcagctacaa tatgtcggcg gcgcttcttc tcgaaggcaa gctggaccgc 25440 acgcgcgtcg agcacgcgtt ccgggcgctg attcagcgcc atgagacgct gcgtaccggg 25500 atcgagcagg ttcaaggcga gcttgtccag cgcatctatg acgaggtgga gtttgctgta 25560 gattatttcc aagcgagcga gtgggaagtg gaccaagtgg tggaagcata ctatcacccg 25620 tttgatctga ccaagccgcc acttctccgc atcggcctga tcgaagtcga cgaggatcgc 25680 cacattctgc tgttcgatat gcaccatatc gtctcggacg gcatttcgac agcgctgctc 25740 ttcgacgagt tcagccgcct gtatcggggc gaggagctgg ccccgctgcg cattcaatac 25800 aaagattatg ccgtttggca gcattccgaa gcttacgggc agatgctcca gccgcagaag 25860 gagtattggc tgaaacagct gtcgggcgag ctgccggtct tggagctgcc gacggacttc 25920 ccgcggcctg cggtgcaaag ctttgacggc cgaaccgtga agttttatat cgagaaagat 25980 cggacggaga agctgaaaga gctggcggca cggacaggga cgaccctgta catggtactg 26040 ctgtcggctt acaccatcct tatgcataaa tattcgggtc aggaagatct gatcgtagga 26100 acgccgattg ccggaagaac gcaggaagaa gtgcagccga tcgtagggat gtttatcaac 26160 acgctggcca ttcgcagccg tccggagcgt tccaagccgt acctttcgta cctggaagaa 26220 atcaaggaca tcacgctcgg ggctttcgaa caccaaaatt atttgttcga agacttggta 26280 gaaagtcttc acattccgcg cgcgaccggc cggaatccgc tctttgacac gttcttctcc 26340 ctacaaaata cggagaacga gcaaattgtc atcgaggggc tggagcaatc gttttatcca 26400 ctggaaaacc aaacatccaa gttcgagctg ctcctggata tttcggagct ggacggtcag 26460 ctcgaatgcc ggttggagta cgctacggct ttgtataaac aagagaccgc ggagcggttc 26520 gccagacatt atgacaagct gctcgaaacc atcgcagcag cgccggacgg ggatattgcc 26580 tcgctggaaa tgctcacgga ggaggaaatc cgcgagttgg tgcgcggttt caacgattcg 26640 gaggcggact acccgcggca gcagacgatt cacggcttgt ttgaagagca ggcggagctt 26700 tatccggaca acgtggccgc cgtcatgaac gagcggcagc tgacctaccg cgagctgaac 26760 gagcgatcca accgccttgc gcggaagctg cgagagacgg gagtagaagc ggaccagctg 26820 gtagcgattc tggccgaacg ctcgctcgat atggttgtcg gcattctggc gattctcaaa 26880 gcgggcggag catacgtgcc tgtcgatccc gactacccgg aggagcgcat ccgcttcatg 26940 atcgaggatt cgggcgcgcc gttattgctg attcaaaagc atctgcacga gaagaccgac 27000 ttcgcaggaa cgcgcctcga attggacgat ttcgttgggg gcgacagagg ggcgaactcc 27060 gccgatgtgc gggacgcttc gaacctggag ccgatttccg ggccgggcaa cctggcttat 27120 gtcatctaca cgtcgggaac gactggcaga ccgaaaggaa cgctgatcga gcataagaac 27180 gtcgtgcgcc tcctgttcaa cgacaagaac ctgttcgact tcgggccgtc cgacacgtgg 27240 acgctgttcc actcgttctg cttcgatttc tccgtctggg aaatgtacgg agcgctgctg 27300 tacggaggca agctggtcat cgtaccgccg ctcacggcga aaagtccagc cgatttcctg 27360 gcgctgctgg gccgcgaaca ggttaccatt ttgaaccaga caccaacgta cttctaccag 27420 ctgctgcgtg aggtcttgac ggatcatcca tacgatttgc ggattcgcaa tgtgatcttt 27480 gggggcgaag cgctgagtcc gctgctgctc aagggcttca agacgaagta cccggagacg 27540 aagctgatca atatgtacgg cattaccgag acgacggttc acgttacgta taaggaaata 27600 acgtgggtcg aaatggaggc ggcgaagagc aatatcggca agccgatccc gacgctgagc 27660 gtgtacgtcc ttgatgaaaa ccgccgccct gtgccgatcg gcgtagcggg cgaaatgtac 27720 gtggccgggg aaggccttgc gagaggatac ctgaaccgcc cggatctgac ggcggagaag 27780 ttcgtcgatt ccccgtttgc ggaaggggag aaactgtacc gctcgggcga cttggcggct 27840 tggctgccgg acggcaacat cgaatacctg ggccgaatcg atcaccaggt caaaatccgc 27900 gggtaccgga tcgagctgga cgaaatcgag acgcagctgc taaacgtcga gggcgtggaa 27960 gaagcggtgg tgcttgcccg tcaggacggc gggggcgaga aggcgcttgt cgcctacttt 28020 gtggcggacc ggacactgac ggtcagcgaa atgagaacct cgctggccca gggaatgccg 28080 gggtacatga tcccgtcgta cttcttgcag cttgagcgca tgccgctgac gtccaacggt 28140 aaagtggacc gcaaagcgct gccggagccg caaggcggct tgcaaacggg cgtcgaatac 28200 gtagcgccgc gtaaccggac ggagtcccag cttgtgaaga tctgggagga agtgctgggt 28260 tactccggca ttggagtcct ggacaatttc ttcgagctcg gaggccactc cttgcgggcg 28320 acgaaccttg tcagcaagat tcggaaggaa atgaacgtcg aacttccact gcgcgatgtg 28380 ttccgctata cgacggtaga gtcgatggcc ggggctattg ccagcttgga agaaacacag 28440 cacagctcga ttccgaaagc ggaagagaaa gcgtactatc cggtttcctc cgcgcaaaaa 28500 aggctgtacg tcctgcacca gctggatagc tcggagctga attacaacct cccaagcgcc 28560 ttgcaattgg aaggggcttt gaacgaggcc aaagtggaaa aggcgctgac tactttggtg 28620 gcccggcacg atatgctgcg caccggtttt gaaatcgtga atggggagcc ggttcagcgt 28680 attcatccgc tcgtagcttt caaggtcgag aagcttcaag caagcgaaga tcaggttgcg 28740 gccattcttg aaggcttcat tcagcctttt gacttgaccc agccgccttt gctgcgcgcc 28800 ctactgatcg aactggagaa agagaaattc ctgctcgcgc tggatattca tcatattggt 28860 tccgacggcc tttccatgga cgtgctgctg cgcgaattcg tgcggcttta caatggggaa 28920 gaattgccgg agctgcggat tcaatacaag gattacgccg tttggcagca atccgaggaa 28980 cagcgccagc gcatcaaacg gcaggaggaa tactggcgcg gggtattcag ctccgagctt 29040 ccggttcttg agctgcctct cgacttctcc cgcccggccg tccagcagtt tgacggtcaa 29100 acgctcacgt ttacgctgga tgcggagaaa agcgaagctc tcaaacggct tgccggcgat 29160 tcgggggcga cgctgtacat gcttctgctg gctgcttact ccgtattgct tcataaatac 29220 gcgggacagg aagatatagt ggtcggcact ccgattgccg cccgttctca cgccgacctg 29280 cagccgatta tcggcatgtt cgtcaatacg ctggcccttc gcttgggtcc ggtggcggag 29340 cggacgttcc tggattactt gcaggaagtg aaggaaacga cgcttggagc ctacgagcac 29400 caggactatc cgttcgagga gctggtggaa gctcttcagg tgagccggga tttaagccgg 29460 aatcctctgt ttgacaccat gttttctttg caaaagcacg aaagcttgga tttaaccctg 29520 gaaggcttgc aatggtcgct gttcgacatc gaggaaaaga cggcaaagtt tgatcttagc 29580 tttgatatcg tggaagccga taacgagctg atttgcaaga tcgagtacgc tacctcgttg 29640 tttagacagg aaacgatggt acggctggcg ggtcattacg agcagctttt ggcgtcgatc 29700 ctggctcagc cgggtgcgcg gatttcggat ttggatatct tgacggacag cgaaaagcat 29760 gatttgctgg tcgggtttga cgtgtcgtct tcggctcttg cgaagcaacc cgccgcagag 29820 ggtacaggtt tggaagcgga tgagtcgtgg agagagagaa cgttccacga gctgttcgag 29880 gagcaggcgg agcgcactcc gggagcgctg gcggttgtct acgaagacag caagctgacg 29940 tatgcggagc tgaacgccaa agcgaatcgt ctggcgtatg cactgcgggc gcgcggggtg 30000 aagccggagc aggtggtcgg cattctggcc ggccgttcgg cggagctgtt gatcggggtg 30060 ctcgccgtat ggaaagcggg cggcgcttat gtgccgctcg acccggacta tccggcggag 30120 cggatcgagt atatgctcgc ggacagcggg gcgtcggtgc tgctcacgca gacccgcctg 30180 ctggagcagg cggaagcttg gcgcagcgac ggagctctag tgctgcaaac ggtgcttgcg 30240 cttgacgacg ccgcgacgta cagcctcgga gcggcggaag cggctgtggg cgaagcaggc 30300 gcagaggcgg aggctttggc gcaagcggct gctgccgaat cgtccgccac ggcagaagcc 30360 gagaagaacg tactagcggc ggatctcgca tcgaatccgg cgaatgtgaa caagccgcgc 30420 gatttggctt acgtcatcta cacctccggt acgacgggcc gtccgaaggg cgtggcggtg 30480 gaaaaccgca gcctggtgaa cacggcggcg ggctaccggc gggaataccg gttggatcag 30540 ttcccggtgc ggctgctgca gctcgcaagc ttctcgttcg acgtgttcgt gggagatatc 30600 gcgcggacgc tgtacaacgg cggcaccatg gtcatcgtgc cgaaggacga ccggattgat 30660 ccaacccgcc tctacggctg gattcgcgac tacgccgtga cggtgttcga atcgactccg 30720 gcgctgatcg tgccgttcat ggagcatgtg catgccgagg gtctggacct cagctcgatg 30780 cagctgctga tcacaagctc ggatgcgtgc agcgtagcgg attaccgcac cttgcaggag 30840 cgcttcggct cgcagttccg tattattaac agctacggcg taacggaagc ggcgattgac 30900 tccagcttct atgacgagcc gctggagaag ctgccgaaga cgggcagcgt gccgatcggg 30960 aaagcgtggc tgaacgccaa gttttacatc gtggatgcga atctgaagcc ggtgccgatc 31020 ggggtgttgg gcgagctggt tatcggcgga gcgggtgtgg cccgaggtta cttgaaccgc 31080 ccggatttga cggcggagaa attcgtagac agcccgttcg ccgctgggga gcggctgtac 31140 cggacgggcg acctggcgcg ctggatgccg gacggcaacg tggacttcat cggccggatc 31200 gacaaccagg tgaaaattcg cggctatcgg atcgagcttg gtgaaattga agcggccatg 31260 aaaaattttg ccggcgtgcg tcaagcgctt gtcatcgacc ggacggacga gcgggggcag 31320 aaatatttgt gcgggtatgt cgtagcggat tccagcttcg atctggaagg gcttgtggcc 31380 catctggacg ccgcgctgcc ttcccatatg gtgccttcgc gcatcatgcg cctggatcaa 31440 atgccgctta cgccgaacgg gaagatcgac cgtaaagcgc tgcctgtacc ggaaggaagc 31500 attcgtgccg aggctgcata cacggcgcct cgtactcctg ccgagcaagc acttgcgtcg 31560 gtctggcagt cagtgctggg cgtggatcag gtcggcacga tggacaattt ctttgcgctc 31620 ggcggcgatt cgatcaaggc cttgcaggta tcgtcccgtc ttttgcaaac ggggtacaag 31680 ctggtcatga aagatttgtt ccattacccg acgatttccg cccttagttt gcagctgcaa 31740 acggcggaga gaacggcaag ccaggccgaa gtgacgggag aggtcatctt gaccccgatt 31800 cagcgttggt tctttgaaca aaatccggcc gacgtgcatc acagcaacca ggcgttcatg 31860 cagttctcca agcaaggctt tgacgaagaa gctttacgcc aagcggtgcg tcagcttgtc 31920 gtgcatcacg atgctctccg tacggtttac cgccaaactg agaacggcta taccgcctgg 31980 aatcgcggcg ccggggagaa cgaagcgctg ttcgatctgg aagttgtcga tttcaaggga 32040 gtcggcgacg tgaaagaagc ggtagaggct aaggcgaatg acattcaagc gagcatcgat 32100 ctggaaaacg gtccgctggt gaagctcggc ttgttccgct gcgacgacgg cgaccacctg 32160 ctcatcgcga tccatcactt ggtcgtagac ggcgtatcct ggcggattct gcttgaggat 32220 tttgctgcct gctatgagca ggcgctgcaa gggcagccga tccgtctgcc gctcaaaacg 32280 gattcattcc aaacgtgggc gaaacagctc gctgattatg cgaacggtcc ggcgatggaa 32340 agcgagagag agtattggca gcatatcgag caattgacct atgagccgct tccgaaagat 32400 tttgaacaag gcagatccaa gctgaaggac agcgggctcg tgaccgttcg ctggacggcg 32460 gaggaaaccg aacagctgct gaagcaggca cactgtgctt accatacgga aatgaacgat 32520 ttgctgcttg ccgcgcttgg cctcgcggta caagcttgga gcggccggga acgcgtgctg 32580 gtgaatctcg aaggccacgg ccgggaagat attttgccga atgtggacat tacgcgcacg 32640 gtaggctggt ttacaagcca attccctgtc gttctggagc cgggtcacgc ccaggcgctc 32700 ggtcatcagg taaaacaggt taaagaaagc ttgcgccgca ttccgaacaa aggaatcggc 32760 tacggcatcc tgcgttattt gtcggcgccg cgtgacggcg agcgcttcgc tttggagccg 32820 gagatcagct ttaactattt gggtcagttc gaccaggatt acgaaagcag cggctcgagg 32880 ccgtctccgt tcagcccggg ctccgattca agcccggatg cagtgatgga ttatgtcctc 32940 gatatcaacg gtatggtgtc ggaaggagtg caggaactca cgatccgtta tggggaaacc 33000 cagtataaac gggaaacggt agagcgcctg ggcaccctgc ttcattcgag cttgcgtgaa 33060 gtcatcagcc attgcgtatc gaaagagcgg ccggagctta cgcctagcga cgtactgctt 33120 caagatgtga cgttggagga actggagcgg ctggccgaac atacggcggc gctcggcgaa 33180 ctggagaatg tatacaccct gactccgctg caaaaaggga tgttgttcca cagcctgctg 33240 gatgccgatt cggaagctta cttcgaacag gtgaccttcg atctgtacgg aagcctgaat 33300 gtcgaagcct tcacccaagg attggatacg ctggtgcagc ggaatgaggc gctgcgtacc 33360 aactttatta ccggctggag agacgagccg attcaagtgg tattccgcga gcggaagtgt 33420 gaagtgtact tcgaagatat tcgctcggta agcgatgaag acccggagaa gacgatagcc 33480 gatttcgtca gcgcggataa agcgaataag ttcgatttgg cccgaggctc tcttatgcgc 33540 gtgacagttt tgcgcacggg cgacgagtcc taccatgtga tctggagtca ccatcacatt 33600 ttgatggacg gctggtgcat gtccttcatg atcaaggaag tgttcgacac ctacttcgct 33660 ttccaggaga agcggacgct ggagcttcct ccggttacct cgtactcccg gtatatcgaa 33720 tggctggaag ctcaagatgc cgcgaaagct tcgcgttact ggtccgagta tttggcgggt 33780 tacgatcagc agaccaagct gccccaggag aaaacgcagc tgaagcaggg cgcttttgaa 33840 gcggctgaaa tcgatgtgga actcagcaag gaactgaccg ggcaaatcga gcgggtggcg 33900 cgccagcagc aggtgacgct caatacgttc atgcagaccg tatggggact ggttctgcag 33960 gtatacaaca acagcgagga tgtcgtattc ggctccgtcg tatccgggcg tccggcggaa 34020 attccgggca tcgaaagcat gatcggcctg tttattaaca cgatcccggt ccgtattcaa 34080 ggcaaagccg aggagacggt agccgatatc ttgagaaaaa cccaggatca agcgctggca 34140 tcgggagctt acgaaacgtt cccgctgttc gaaattcagt cgctgagcga gcacaagcgc 34200 gacttgatca accatatcat ggtctttgaa aattatccga tggaagaaca gattgagcag 34260 gtcgtcggcg gagacaaaga agcgctgaaa atcgctaata tccagtcgcc ggagcaaacg 34320 aactacgacc tggacattac cgtcattccg gaagagcata ttttgctgcg gtttacgtac 34380 aatgcgctga cgttcagaga ggaagacatc aggctgatcc acggtcattt tgcccgggta 34440 ctggagaagg ttgcggctaa cccgagcatc cgcgtgaatc agttggagct tttgacggcg 34500 gcggaaaaag agcaaattct tggcgcgttt aacccggcgc agccggaagc ggctccggcg 34560 gccgcgttcc accggctgtt cgaggaacag gcggagcgca cgccggaagc ggcagctgtc 34620 gtgtacgaga atgaccggct gacgtatgag gagctgaacg agcgggcgaa tcgcttggcg 34680 gccacgctgc gcgcaagcgg catcggccgg gagacgatcg tcggcattct cgccgagcgt 34740 tcggtggact tgctggtggc cgtgctggcc gtctggaaag cgggcggggc gtatgtgccg 34800 ctcgacccgg attatccggc ggagcgggtg cggttcatgc tcgaagacag cggagcgaag 34860 gtgctgctga cgcaaacgcc gctgcgagag cgcgccgaag cctggctcgg cgaagaggag 34920 ctggcgctgg cggcggtgct gtacctcgac gacgaaactt cgtacagcga ggagcggacg 34980 aatgccgaca gcttccatga agcccgtcca gaggacctgg cgtacgtgat ctacacgtcg 35040 ggaacgacgg gcaagccgaa gggcgtgatg atcgagcacc gcagcctggt gaacacggcg 35100 gcgggctacc ggcgggaata ccggttggat cagttcccgg tgcggctgct gcagctcgca 35160 agcttctcgt tcgacgtgtt cgtgggagat atcgcgcgga cgctgtacaa cggaggtacg 35220 atggtgattg tgccgaagga cgaccggatc gatccgtctc gtctgcacca ctggatggag 35280 cgggagcggg tcaccatctt cgaatcgacg ccggcgctga tcgtgccgtt cctggagtac 35340 gtgcacgatc agcggctgga tatgagctgg atggagctgt tgatcacgag ctcggacagc 35400 tgcagcgtgg cggattaccg gaccttgcag gaacgcttcg gctcgttgtt ccggatcatc 35460 aacgcatacg gcgtgacgga agcggcgatc gactcaagct tctatgacga ggagctggcg 35520 aagctgccgc agacaggcaa tgtgccgatc ggcaaagcgt ggctgaatgc gaaattctac 35580 atcgtggacg cgcatctgaa cccggtgccg gtcggggtgc tgggcgagct ggttatcggc 35640 ggagtcgggg tagcgcgcgg gtacttgaac cgtccggagc tgacagaaga gaagttcgta 35700 gacagcccgt tcgccgcggg cgagcggctg taccgcacgg gagacttggc gcggtggatg 35760 gaggacggga acgtggactt catcggccgg atcgacaacc aggcgaaaat ccgggggtac 35820 cggattgaga cgggcgaagt cgaagcgaag ctgctgagtg taggtggcgt gaaggaagcg 35880 gtcgtcgtcg tcagggaaga tcaagaaggt cagaaagctt tgtgcgctta ttatacagcg 35940 gaagaaggct tgacggcggc ggacctgaag cgggcgattg ccaacgagct gccggggtac 36000 atgatcccgt cgtacttcgt ggagctggag cacctgcctc tgacgccgaa cgggaaaatc 36060 gaccggaagg cgctgccggc gccggaaggg ggagcaagcg caggccgcga atacgtggcg 36120 ccgcgcaccg aacttgaggc gaagctggcc gccatttggc aggatgtgct tgttcgggag 36180 aaggcggtag gcgtaacgga caacttcttc gacctcggcg gacactccct gcgggcgacg 36240 acgcttgtca gcaaaatgca taaggagcta ggcgttgaat ttccgcttcg cgacgtattc 36300 cgctactcga cggttgagga aatggccgcg gctatggagc ggctggagat cggctcgttc 36360 acggctattc cggctgcgga acctagcgag tattatccgc tctcttccgc tcagaaacgt 36420 ctctatatct tgaaccagct ggaaggagcc gagctgagct acaacatacc gggagctatg 36480 ctgctcgaag gggagctcga ccggcagcgg tttgaagaag cgttccgcgg gctcgtagct 36540 cgtcatgaaa cgctgcgtac cggctttgag atggtaaaag gcgaagcggt tcagcggatt 36600 tacgaagaag ccgccttcca ggtggaatat gtgcagatca gcgcggagca ggcggaagaa 36660 acggtgcgcc aattcgttcg tccgtttgat ctagcgaagc cgccacttct gcgggtgggc 36720 cttgccgaac tggcgccgga ccggcacatt ctaatgttcg atacgcatca tatcgtatct 36780 gacggcgttt cgattgacgt actgatcgaa gagttcgtcc gcttgtacag cggggagcag 36840 ttggagccgc tccgcattca gtacaaagat tatgcggtat ggcagcaatc ggacgagcag 36900 aaagctcagc ttgccaagca ggaagcctac tggctcgaca tgttccgcgg agagctgccg 36960 gttttggaat tgccgatgga ctatccgcgc ccggctatgc agagctacga gggccgcacg 37020 ctgcaattgt ttatgcatag cgagaaaagc gagggtctga aacggcttgc agccgagaac 37080 ggcgcaacgc tttacatggt tctgcttgct ggttatacaa tattattgca taaatataca 37140 gggcaagaag acgtggtggt cggtacgccg attgcgggaa gaaatcacag cgacgttcag 37200 ccgctgatcg ggatgttcgt caataccctg gccatccgca gttatccggc tgccggtaag 37260 acgttccttg actacttgaa ggaaatcaag gagacgacgc tgggcgcttt tgaacatcag 37320 aattatccgt ttgaggaact ggtggataag gtgaacgtgg ctcgcgattt aagccgcaat 37380 ccgctgttcg atacgatgtt tgctttgcag aatacagaga atttggaaat ccagcttccc 37440 ggactccatt tgtcgacgta tgccagcgaa gaaattgttt ctaaattcga tctcagcttg 37500 gacgtcacgg agatcgagga aggcttggaa tatctgtttg aatacgccac tgctctttat 37560 aaaaccgaaa cggtggagaa attggccgct cactacttgc agctgcttga atccattctc 37620 cgcaacccgt cggcgactat tgccgagctg ggcattttga caccggcgga aaaagagcaa 37680 attctcggcg cgtttaaccc ggcgcagccg ggagcggctc ctgcggctgc gttccaccgg 37740 ctgtttgagg aacaggcgga gcgcacgccg gaagaggcgg ccgtcgtgta cgagaacgac 37800 cggctgacgt atgcggagct gaacgagcgg gcgaaccgct tggcggctac gctgcgcgca 37860 agcggcatcg gccgggagac gatcgtcggc attctcgccg agcgttcggt ggacttgctg 37920 gtggccgtgc tggccgtctg gaaagcgggc ggggcgtatg taccgctcga cccggattat 37980 ccggcagacc gcgtgcggtt catgcttgaa gacagtggag cgaaggtgct gctgacgcaa 38040 acgccgctgc gagagcgtgc cgaagcctgg ctcgacgaag aggagctggc gctggcggcg 38100 gtgctgtacc tggacgacga agcgtcgtac agcgaggagc gggcgaatgc gtcggttgtc 38160 tccggcattg tctccggcat tgtctccggc aagctgacgg atgctgtgga cgacggcgat 38220 gagagccatc agaatgttgg caccgacagc ttccatgaag cccgtccgga ggatctggcg 38280 tacgtgatct atacgtcggg aacgacgggc aagccgaagg gcgtgatgat cgagcaccgc 38340 agcctggtga acacggcggc gggctaccgt cgggaatacc ggttggatca gttcccagtg 38400 cgcctgctgc agctcgcaag cttctcgttt gacgtgttcg tgggagatat cgcccggacg 38460 ctgtacaacg gaggtacgat ggtgatcgtg ccgaaggacg accggatcga tccgtctcgt 38520 ctgcaccact ggatggagcg ggagcgggtc accatcttcg aatcgacgcc ggcgctgatc 38580 gtgccgttcc tggagtacgt gcacgagcag caactggata tgagctggat ggagctgttg 38640 atcacgagct cggacagctg cagcgtggcg gattaccgga ccttgcagga acgcttcggc 38700 tcgttgttcc ggatcatcaa cgcatacggt gtgacggaag ctgcgatcga ctcaagcttc 38760 tatgacgagg agctggcgaa gctgccgcag acaggcaatg tgccgatcgg caaagcgtgg 38820 ctgaatgcga aattctacat cgtggacgcg catctgaacc cggtgccggt cggagtgctg 38880 ggcgagctgg ccatcggcgg agtcggagtg gcgcgcgggt acttgaaccg tccggagctg 38940 acggaagaga agttcgtaga cagcccgttc gccgcgggcg agcggctgta ccgcacggga 39000 gacttggcgc ggtggatgga ggacgggaac gtggacttca tcggccggat cgacaaccag 39060 gcgaaaatcc gggggtaccg gattgagacg ggcgaagtcg aagcgaagct gctgagtgta 39120 ggtggcgtga aggaagcggt cgtcgtcgtc agggaagatc aagaaggtca gaaagctttg 39180 tgcgcttatt atacagcgga agaaggcctg acggcggcgg acctgaaacg ggcgattgcc 39240 agcgagctgc cggggtacat gatcccgtcg tacttcgtcg agctggagcg cctgcctctg 39300 acgccgaacg ggaaaatcga ccggaaggcg ctgccggcgc cggaaggggg agcaagcgca 39360 ggccgcgaat acgtggcgcc gcgcaccgaa ctggaggcga aactggtcgc catctggcag 39420 gacgtgctcg ggccggtcac gattggcgtg acggacaact tcttcgacct cggcgggcac 39480 tccctgcggg cgacgacgct ggtcagcaag gtgcacaagg agctgagcgt ggacctgccg 39540 ctgcgcgatg tgttccggca ctcgaccatc gaagcgatgg ccgaagcgat cagccaattg 39600 gagcggcagg aacacctctc cattccggtt ctggataaga gggattacta tccgctttcc 39660 tccgtgcaaa aacggctgta catccagcag cagatggaag gcgccgagct tagctacaac 39720 atgtccggca tgacggttct cgtcgggcgc ttggaacgga atcaattcga ggcggcgctc 39780 aaaggattga tagctcgtca cgaaattttg cgaaccggct tcgaaatggt cgacggcgaa 39840 ccggtacaac ggatttatcc ggacttgaag tttgccgtcg agtatacgaa agcgacggaa 39900 agtgaaacga agagcatcgt agacggcttt gtgcgcgtct ttgatttgga gcggccgccg 39960 ctgctgcgtg tgggcttagt cgaatgggaa gcggaacggc atctgctcat gctggacatt 40020 catcatatcg tcacggatgg catgtcgatg ggcatcttcg tcgaagagct gctgcgcctg 40080 tataacggcg agactatgga accgcttcgg attcaataca aggaattcgc cgcttggcag 40140 cagtccgaac ctgtaaaaga gcggctgaaa cgtcaggaag cctactggct ggacgtgctg 40200 gaaggcgaac tgccgacgct tgaactgcct acggactttg tcagacctgc cgctcgcagc 40260 tttgagggag atgtgctgcc tttcagcatc gacaagcaga tgaccgacag cttgcagcgc 40320 atcgccgatg agaacggtgc caccctttat atggtgttat tggcggccta ttcaatcctg 40380 ctcagcaagt actcgggaca agaagacttc attgtaggca cgccggtttc gggccgcaca 40440 catgccgacc tggagccgct catcggaatg tttgtcaaca ctttggcgat tcgccattat 40500 ccgtccgggg agaagacgtt cctcgcttac ttgaacgaag tcaaagaaac gatgctgggg 40560 gcctacgacc accaggatta tccgttcgag gagcttgtga aaaagctgca ggcgccgcga 40620 gatcaaggcc gcaatcctgt attcgatgtc atgtttgctc tggaaaccaa ggaagataac 40680 gttcaaagct tcggggatat caagatcgaa tcttatccgg aaactcatac ggtttcccaa 40740 tttgatctta ccttggtcat ttcgttgctg gatgagggaa tgaacgggca gtttgaatat 40800 gccaccaagt tgtttacacg caatctgatc gacaatttcg ctcaggacct gctcgtgatc 40860 atcacccaaa tttgcgaaca gccttcggtg ctgttgaagg atatttccct gaacgggcaa 40920 tccgaacagg agcaagatgt gctagagacc attgatatta ttttc 40965 <110> Korea Research Institute of Bioscience and Biotechnolgy <120> Polymyxin B or E synthetase and gene cluster thereof <130> 11p-12-54 <160> 20 <170> PatentIn version 3.5 <210> 1 <211> 4959 <212> PRT <213> Paenibacillus polymyxa F4 <400> 1 Met Ala Phe Glu Lys Glu Thr Leu Phe Trp Asn Glu Lys Phe Gly Ser 1 5 10 15 Asp Asp Tyr Thr Leu Thr Arg Leu Pro Tyr Ser Lys Ala Pro Ser Ser 20 25 30 Leu Ala Pro Ile Met Thr Thr Val Gly Gly Thr Leu Ser Glu Glu Val 35 40 45 Ala Gln Arg Val Leu Gln Met Ser Lys Gly Ala Pro Leu Ala Thr Phe 50 55 60 Met Ile Leu Leu Ala Gly Val Gln Ser Leu Leu His Lys Tyr Thr Gly 65 70 75 80 Ala Ser Asp Ile Leu Val Gly Met Pro Val Val Arg Lys Pro Thr Glu 85 90 95 Thr Arg Arg Ser Val Asn His Thr Ile Ile Leu Lys Asn Ser Leu Ser 100 105 110 Ala Gly Ala Thr Phe Lys Thr Leu Leu Ser Glu Leu Arg Thr Ser Leu 115 120 125 Pro Glu Ala Ile Gln His Gln His Ile Pro Phe Leu Lys Met Thr Glu 130 135 140 Lys Leu Asp Leu Gln Tyr Ala Gly Gly Ile Pro Val Val His Thr Leu 145 150 155 160 Val Ser Leu Lys Glu Leu His Leu Asp Glu Ile Gly Gln Asn Val Val 165 170 175 Thr Asp Cys Ser Phe Glu Phe Ser Leu Thr Gly Gly Thr Ile Gln Leu 180 185 190 Ala Leu Ser Tyr Asn Glu His Leu Tyr Asp Ser Glu Phe Met Thr Arg 195 200 205 Val Val Gly His Leu Asn Arg Leu Leu Ala Val Gly Leu His Glu Leu 210 215 220 Glu Leu Asp Ile Val Arg Ala Asp Met Leu Ser Glu Asp Glu Lys Phe 225 230 235 240 Gln Leu Leu Gln Ser Phe Asn Asp Thr Glu Lys Asp Tyr Pro Arg Asp 245 250 255 Arg Thr Ile His Gln Leu Val Glu Glu Gln Ala Lys Arg Val Pro Glu 260 265 270 Ala Thr Ala Val Val Phe Glu Gly Arg Arg Leu Ser Tyr Ala Glu Leu 275 280 285 Asn Glu Arg Ala Asn Arg Leu Ala Arg Thr Leu Arg Ser Val Gly Val 290 295 300 Leu Pro Asn Gln Leu Val Gly Met Met Ala Arg Arg Ser Leu Glu Thr 305 310 315 320 Val Val Gly Ile Leu Ala Val Leu Lys Ala Gly Gly Ala Tyr Val Pro 325 330 335 Ile Asp Pro Glu Tyr Pro Glu Glu Arg Ile Arg Tyr Ile Leu Glu Asn 340 345 350 Ser Asn Ala Gln Leu Leu Leu Thr Gln Arg Glu Leu Leu Gln Gln Val 355 360 365 Pro Phe Glu Gly Thr Val Val Ala Leu Asp Asp Glu Gln Ala Tyr Ser 370 375 380 Asp Asp Gly Ser Asn Leu Glu Pro Ala Ser Gly Pro Asn Asp Leu Ala 385 390 395 400 Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly Lys Pro Lys Gly Val Met 405 410 415 Leu Glu His Arg Gly Leu Val Ser Leu Lys Leu Thr Phe Ala His Thr 420 425 430 Leu His Thr Thr Glu Gln Asp Arg Val Leu Gln Phe Ala Ser Leu Ser 435 440 445 Phe Asp Ala Ser Cys Trp Glu Met Phe Asn Ala Leu Tyr Phe Gly Ala 450 455 460 Thr Leu Tyr Ile Pro Ser Thr Glu Thr Ile Leu Asp Asp Gln Leu Phe 465 470 475 480 Glu Arg Phe Met Asn Glu His Ala Ile Thr Ile Ala Thr Leu Pro Pro 485 490 495 Thr Tyr Ala Ala Tyr Leu Asn Ser Asp Arg Leu Pro Ser Leu Ser Arg 500 505 510 Leu Ile Thr Ala Gly Ser Ala Val Ser Ala Glu Phe Val Gln Gln Trp 515 520 525 Lys Asp Lys Val Gln Tyr Tyr Asn Ala Tyr Gly Pro Thr Glu Ala Ser 530 535 540 Ile Ala Thr Ser Val Trp Ala Ala Ser Thr His Asp Thr Glu Arg Arg 545 550 555 560 Ala Ile Pro Ile Gly Arg Pro Ile Trp Asn His Arg Leu Tyr Ile Leu 565 570 575 Gly Ala Gln Asn Gln Leu Ala Pro Ile Gly Val Glu Gly Glu Leu Cys 580 585 590 Ile Ala Gly Val Gly Leu Ala Arg Gly Tyr Trp Asn Arg Pro Asp Leu 595 600 605 Thr Ala Glu Lys Phe Ile Glu His Pro Phe Glu Pro Gly Glu Arg Leu 610 615 620 Tyr Arg Thr Gly Asp Leu Ala Arg Trp Leu Pro Asp Gly Asn Ile Glu 625 630 635 640 Tyr Leu Gly Arg Ile Asp His Gln Val Lys Ile Arg Gly Phe Arg Ile 645 650 655 Glu Ile Gly Glu Ile Glu Glu Gln Leu Leu Lys Ile Asp Ser Val Gln 660 665 670 Glu Thr Ile Val Ile Ala Arg Glu Gly Lys Ser Gly Gln Glu Leu Cys 675 680 685 Ala Tyr Leu Val Ala Gly Arg Pro Leu Thr Leu Gly Glu Leu Arg Ser 690 695 700 Ala Leu Ala Gln Lys Leu Pro Asn Tyr Met Ile Pro Ala His Phe Val 705 710 715 720 Gln Leu Pro Gln Met Pro Leu Thr Pro Asn Asp Lys Ile Asp Arg Lys 725 730 735 Ala Leu Pro Ala Pro Glu Gly Asn Ala Leu Thr Gly Gly Ala Tyr Val 740 745 750 Ala Pro Arg Asn Glu Ala Glu Arg Thr Leu Ala Asp Val Trp Gln Ala 755 760 765 Val Leu Asn Ala Asp Arg Val Gly Val Thr Asp His Phe Phe Glu Leu 770 775 780 Gly Gly Asp Ser Ile Lys Ser Ile Gln Val Ser Ser Arg Leu His Gln 785 790 795 800 Ala Gly Tyr Lys Leu Glu Ile Arg Asp Leu Phe Lys Tyr Pro Thr Ile 805 810 815 Ser Gln Leu Ser Leu His Val Lys Pro Ile Gly Arg Thr Ile Asp Gln 820 825 830 Gly Glu Ile Thr Gly Glu Thr Ala Leu Met Pro Ile Gln His Trp Phe 835 840 845 Phe Glu Ser Ser Phe Ala Asp Pro His His Phe Asn Gln Ser Val Met 850 855 860 Leu Tyr Arg Lys Glu Arg Phe Asp Glu Glu Thr Val Arg Gln Val Leu 865 870 875 880 Gln Lys Leu Ala Glu His His Asp Ala Leu Arg Met Val Phe Arg Lys 885 890 895 Thr Glu Gln Gly Phe Ser Ala Arg Asn Arg Ala Ile Gln Glu Gly Gly 900 905 910 Leu Phe Thr Leu Asp Val Phe Asp Phe Lys Asp Ala Glu Asn Thr Ala 915 920 925 Gln Ala Val Glu Ala Lys Ala Thr Asp Ile Gln Ala Gly Ile Asp Leu 930 935 940 Glu Asn Gly Pro Leu Ala Lys Ala Gly Leu Phe Arg Cys Ala Asp Gly 945 950 955 960 Asp His Leu Leu Leu Ala Val His His Ala Val Val Asp Gly Val Ser 965 970 975 Trp Arg Ile Leu Met Glu Asp Phe Ala Leu Gly Tyr Glu Gln Ala Gly 980 985 990 Lys Ser Glu Glu Ile Arg Phe Pro Ala Lys Thr Asp Ala Tyr Arg Thr 995 1000 1005 Trp Ser Glu Gln Leu Ala Ala Tyr Ala Gln Ser Pro Glu Met Ala Lys 1010 1015 1020 Glu Arg Ala Tyr Trp Gln Ala Val Glu Gln Ile Ala Val Pro Ala Val 1025 1030 1035 1040 Pro Lys Asp Leu Glu Ala Asp Val Thr Thr Gln Gln Asp Ser Glu Ser 1045 1050 1055 Leu Phe Val Arg Leu Thr Pro Glu Glu Thr Glu Leu Leu Leu Lys Arg 1060 1065 1070 Val His Arg Ala Tyr Asn Thr Glu Met Asn Asp Ile Leu Val Thr Ala 1075 1080 1085 Leu Gly Ile Ala Val Arg Lys Trp Thr Gly His Glu Arg Val Arg Ile 1090 1095 1100 Asn Leu Glu Gly His Gly Arg Glu Ser Ile Gly Thr Asp Ile Asp Ile 1105 1110 1115 1120 Thr Arg Thr Val Gly Trp Phe Thr Thr Lys Phe Pro Val Val Leu Glu 1125 1130 1135 Pro Glu Thr Asp Arg Asp Leu Ala Tyr Gln Ile Lys Gln Val Lys Glu 1140 1145 1150 Ser Leu Arg Arg Ile Pro Asn Lys Gly Leu Gly Tyr Gly Val Cys Arg 1155 1160 1165 Tyr Leu Ser Lys Ser Glu Asp Gly Phe Val Trp Gly Ala Glu Pro Glu 1170 1175 1180 Ile Asn Phe Asn Tyr Leu Gly Gln Phe Gly Asp Asp Val Asn Gln Asp 1185 1190 1195 1200 Glu Ile Gly Ile Ser Ser Tyr Ser Ser Gly Ser Pro Ala Ser Asp Arg 1205 1210 1215 Gln Ala Arg Ser Phe Val Leu Asp Ile Asn Gly Met Val Leu Asp Gly 1220 1225 1230 Ala Leu Ser Leu Asp Leu Ser Tyr Ser Arg Lys Gln Tyr Arg Lys Glu 1235 1240 1245 Thr Met Glu Ala Phe Ala Gln Arg Leu Glu Gln Ser Leu Arg Glu Leu 1250 1255 1260 Ile Thr His Cys Ala Gly Lys Glu Asn Thr Glu Leu Thr Pro Ser Asp 1265 1270 1275 1280 Val Gln Phe Lys Gly Leu Thr Ile Ala Glu Leu Glu Gln Ile Ala Gln 1285 1290 1295 Arg Ser Gly His Leu Gly Glu Ile Glu Asn Ile Tyr Ser Leu Thr Pro 1300 1305 1310 Met Gln Lys Gly Met Trp Phe His Ser Ala Leu Asp Arg Gln Thr Ala 1315 1320 1325 Ala Tyr Phe Glu Gln Thr Arg Phe Thr Met Arg Gly Ala Leu Asp Val 1330 1335 1340 Gln Leu Phe Glu Arg Ser Trp Met Glu Leu Ala Lys Arg His Leu Val 1345 1350 1355 1360 Leu Arg Ala Asn Phe Val Lys Gly Pro Ala Gly Glu Pro Leu Gln Ile 1365 1370 1375 Ile Tyr Arg Asp Lys Pro Val Gly Phe Glu Tyr Glu Glu Leu Leu His 1380 1385 1390 Leu Gln Ala Asp Glu Lys Gln Ala Tyr Leu Asp Lys Lys Ala Glu Asp 1395 1400 1405 Asp Lys Leu Arg Gly Phe Asp Met Glu His Asp Ala Leu Val Arg Val 1410 1415 1420 Thr Ile Leu Arg Thr Glu Glu Gln Ser Tyr His Val Leu Trp Ser Phe 1425 1430 1435 1440 Gln His Ile Leu Met Asp Gly Trp Cys Leu Pro Gln Leu Thr Gln Glu 1445 1450 1455 Leu Phe Glu Thr Tyr Ser Ala Leu Ala Ser Gly Lys Gln Pro Ala Gly 1460 1465 1470 Asp Lys Gly Ser Asp Tyr Gly Ala Tyr Ile Glu Trp Leu Glu Lys Gln 1475 1480 1485 Asp Asp Gln Ala Ala Ser Gly Tyr Trp Thr Ala Phe Leu Ala Gly Tyr 1490 1495 1500 Glu Gly Gln Thr Val Leu Pro Gly Gln Lys Glu Pro Ala Pro Asn Gly 1505 1510 1515 1520 Arg Phe Thr Ala Asp His Val Thr Ala Glu Leu Gly Lys Asp Leu Ser 1525 1530 1535 Glu Arg Met Asp Arg Val Ala Lys Gln Arg Leu Val Thr Val Asn Thr 1540 1545 1550 Leu Leu Gln Ala Ala Trp Gly Val Met Leu Gln Lys Tyr Asn Gly Thr 1555 1560 1565 Asn Asp Ala Val Phe Gly Ser Val Val Ala Gly Arg Pro Ala Glu Ile 1570 1575 1580 Pro Gly Ile Glu Ser Met Ile Gly Leu Phe Ile Asn Thr Val Pro Val 1585 1590 1595 1600 Arg Val Thr Ser Glu Ala Asp Thr Val Phe Ala Asp Leu Met Ala Lys 1605 1610 1615 Leu Gln Glu Arg Ala Leu Glu Ser Gly Arg Tyr Asp Tyr Tyr Pro Leu 1620 1625 1630 Tyr Glu Ile Gln Ala Arg Ser Val Gln Lys Gln Asn Leu Ile Asn His 1635 1640 1645 Ile Ile Ala Phe Glu Asn Tyr Pro Val Asp Glu Gln Met Glu Gln Ala 1650 1655 1660 Gly Asp Gln Gln His Gly Asp Leu Thr Ile Ala Asp Val Gln Met Glu 1665 1670 1675 1680 Glu Gln Thr Asn Tyr Asn Phe Asn Val Thr Val Val Pro Gly Val Glu 1685 1690 1695 Ile Glu Ile Arg Phe Asp Phe Asn Ala Glu Val Phe Asp Lys Asp Ser 1700 1705 1710 Ile Glu Arg Leu Lys Gly His Leu Val His Leu Leu Glu Gln Val Thr 1715 1720 1725 Asp Asn Pro Glu Ile Thr Val Gly Glu Leu Glu Leu Val Thr Glu Ala 1730 1735 1740 Glu Lys Ala Asp Leu Leu Gly Arg Phe Asn Asp Thr Thr Thr Glu Phe 1745 1750 1755 1760 Pro Arg Gly Lys Thr Leu Ile Gln Leu Phe Glu Glu Gln Ala Glu Arg 1765 1770 1775 Ile Pro Asp Ala Ala Ala Ile Ser Leu Asn Glu Gln Glu Leu Thr Tyr 1780 1785 1790 Arg Glu Leu Asn Glu Arg Val Asn Arg Leu Ala Arg Thr Leu Arg Ser 1795 1800 1805 His Gly Ile Ser Lys Gly Arg Leu Val Ala Ile Met Ala Glu Arg Ser 1810 1815 1820 Ile Glu Met Val Val Gly Met Leu Ala Ala His Lys Ala Gly Ala Ala 1825 1830 1835 1840 Tyr Val Pro Ile Asp Pro Glu Tyr Pro Glu Glu Arg Ile Arg Phe Leu 1845 1850 1855 Ile Glu Asp Ser Gly Ala Gln Val Met Leu Thr Gln Ser Arg Leu Arg 1860 1865 1870 Glu Arg Leu Ala Gly Ser Asp Ser Val Ile Leu Leu Asp Asp Glu Ser 1875 1880 1885 Phe Tyr His Glu Asp Gly Thr Asn Leu Asn Pro Gly Thr Glu Ala Thr 1890 1895 1900 Asp Leu Ala Cys Val Ile Tyr Thr Ser Gly Thr Thr Gly Lys Pro Lys 1905 1910 1915 1920 Gly Asn Leu Val Ser His Arg Asn Ile Val Arg Ile Val Arg Asn Thr 1925 1930 1935 Asn Tyr Ile Asp Ile Thr Glu Arg Asp His Val Leu Gln Leu Ser Ser 1940 1945 1950 Tyr Ser Phe Asp Gly Ala Ile Phe Asp Ile Phe Gly Ala Leu Thr Asn 1955 1960 1965 Gly Ala Arg Leu Val Leu Val Pro Arg Glu Thr Leu Leu Glu Ile Val 1970 1975 1980 Arg Leu Ala Asp Leu Ile Gln Arg Glu Arg Ile Ser Val Met Leu Ile 1985 1990 1995 2000 Thr Thr Ala Phe Phe Asn Val Leu Val Asp Val Asn Val Asp Cys Leu 2005 2010 2015 Arg Asp Val Arg Ala Ile Leu Phe Gly Gly Glu Arg Val Ser Val Gly 2020 2025 2030 His Val Arg Lys Ala Leu Ala His Ile Gly Pro Gly Arg Leu Asn His 2035 2040 2045 Leu Tyr Gly Pro Ser Glu Ser Thr Val Tyr Thr Thr Tyr Leu Pro Val 2050 2055 2060 Asp Phe Val Asp Glu Ser Ala Val Thr Val Pro Ile Gly Arg Pro Ile 2065 2070 2075 2080 Ser Asn Thr Lys Val Tyr Ile Val Asp Ser Arg Asn Lys Leu Leu Pro 2085 2090 2095 Ile Gly Val Ala Gly Glu Leu Cys Val Gly Gly Glu Gly Leu Val Arg 2100 2105 2110 Gly Tyr Asn Asn Arg Pro Glu Leu Thr Ala Glu Lys Phe Val Asp Asn 2115 2120 2125 Pro Phe Val Leu Gly Glu Arg Met Tyr Arg Thr Gly Asp Leu Ala Lys 2130 2135 2140 Trp Leu Pro Asp Gly Thr Ile Glu Tyr Val Gly Arg Thr Asp Asp Gln 2145 2150 2155 2160 Val Lys Ile Arg Gly Phe Arg Ile Glu Leu Gly Glu Ile Glu Ala Gln 2165 2170 2175 Leu Gln Lys Val Glu Gly Ile Arg Lys Ala Thr Val Phe Ala Arg Glu 2180 2185 2190 Asp Ala Ser Gly Glu Lys Gln Leu Cys Ala Tyr Tyr Glu Ala Asp Arg 2195 2200 2205 Glu Leu Pro Ala Ala Glu Leu Lys Ser Val Leu Ser Gln Glu Leu Pro 2210 2215 2220 Ala Tyr Met Ile Pro Ala Tyr Leu Ile Gln Leu Glu Gln Leu Pro Leu 2225 2230 2235 2240 Thr Thr Asn Gly Lys Val Asp Arg Arg Ser Leu Pro Ala Pro Glu Glu 2245 2250 2255 Ser Leu Gln Pro Gly Glu Glu Arg Thr Pro Pro Arg Thr Pro Leu Glu 2260 2265 2270 Ala Ser Leu Ala Gly Ile Trp Lys Ser Val Leu Gly Leu Glu His Ile 2275 2280 2285 Gly Val His Asp Asn Phe Phe Asp Leu Gly Gly His Ser Leu Arg Ala 2290 2295 2300 Thr Thr Leu Val Ser Lys Val His Gln Glu Leu Asn Val Glu Leu Pro 2305 2310 2315 2320 Leu Arg Asp Val Phe Arg Tyr Ser Thr Ile Glu Glu Met Ala Leu Ala 2325 2330 2335 Ile Ser Arg Ile Gly Glu Gln Ser Phe Ser Ser Ile Pro Leu Ala Gly 2340 2345 2350 Ala Arg Ala Tyr Tyr Pro Leu Ser Ser Ala Gln Lys Arg Leu Phe Ile 2355 2360 2365 Leu Asn Gln Leu Glu Gly Ala Asp Gln Ser Tyr Asn Met Pro Gly Val 2370 2375 2380 Leu Leu Leu Glu Gly Ser Ile Asp Arg Ser Leu Leu Glu Lys Ala Phe 2385 2390 2395 2400 Arg Gly Leu Ile Ala Arg His Glu Thr Leu Arg Thr Gly Phe Glu Ile 2405 2410 2415 Val Gln Gly Glu Ala Val Gln Arg Ile Tyr Glu Ser Val Asp Phe Ala 2420 2425 2430 Val Gly Tyr Arg His Val Ser Glu Glu Glu Ala Pro Glu Val Val Gln 2435 2440 2445 Ala Phe Ile Arg Pro Phe Asp Leu Ala Lys Pro Pro Leu Leu Arg Ala 2450 2455 2460 Glu Leu Val Glu Leu Ala Ala Glu Arg Tyr Leu Leu Met Phe Asp Met 2465 2470 2475 2480 His His Ile Val Ser Asp Gly Val Ser Met Asp Val Leu Val Glu Glu 2485 2490 2495 Leu Val Arg Leu Tyr Gly Gly Glu Ser Leu Glu Pro Leu Arg Ile Gln 2500 2505 2510 Tyr Lys Asp Tyr Ala Val Trp Gln Gln Ser Asp Glu Gln Lys Val Gln 2515 2520 2525 Leu Lys Arg Glu Glu Ala Tyr Trp Leu Asp Arg Tyr Arg Gly Glu Leu 2530 2535 2540 Pro Val Leu Glu Met Pro Thr Asp Tyr Pro Arg Pro Ala Val Gln Ser 2545 2550 2555 2560 Phe Glu Gly Gln Thr Leu Thr Ser Phe Val Asp Glu Ala Thr Asn Glu 2565 2570 2575 Gly Leu Lys Gln Leu Ala Ala Gln Arg Gly Thr Thr Leu Tyr Met Val 2580 2585 2590 Leu Leu Ala Ala Tyr Thr Val Leu Leu His Lys Tyr Thr Gly Gln Asp 2595 2600 2605 Asp Leu Ile Val Gly Thr Ser Ile Ala Gly Arg Thr His Gly Glu Thr 2610 2615 2620 Gln Pro Leu Ile Gly Met Phe Val Asn Thr Leu Ala Leu Arg Asn Tyr 2625 2630 2635 2640 Pro Ala Ser Glu Lys Thr Phe Leu Ser Tyr Leu Glu Glu Val Lys Glu 2645 2650 2655 Thr Thr Leu Gly Ala Tyr Glu His Gln Asn Tyr Pro Phe Glu Glu Leu 2660 2665 2670 Val Asp Lys Val Gln Val Ser Arg Asp Leu Ser Arg Asn Pro Leu Phe 2675 2680 2685 Asp Thr Met Phe Ser Leu Gln Asn Leu Glu Asp Lys Glu Phe Glu Leu 2690 2695 2700 Glu Gly Leu Lys Leu Ser Pro Tyr Pro Ser Glu Tyr Gly Thr Ala Lys 2705 2710 2715 2720 Phe Asp Leu Ser Val Asp Val Thr Glu Glu Asn Gly Gly Leu Glu Cys 2725 2730 2735 Ser Phe Glu Phe Ala Thr Ala Leu Tyr Lys Glu Ser Thr Ile Arg Arg 2740 2745 2750 Leu Ser Thr His Phe Gly His Leu Leu Val Thr Ile Val Ser Arg Pro 2755 2760 2765 Asp Ala Lys Ile Ala Glu Leu Asn Leu Leu Thr Ala Glu Glu Glu Lys Glu 2770 2775 2780 Gln Ile Leu Gly Ala Phe Asn Pro Ala Gln Pro Glu Ala Ala Pro Ala 2785 2790 2795 2800 Ser Ala Phe His Arg Leu Phe Glu Glu Gln Ala Glu Arg Thr Pro Glu 2805 2810 2815 Ala Glu Ala Val Val Tyr Glu Asn Asp Arg Leu Thr Tyr Ala Glu Leu 2820 2825 2830 Asn Glu Arg Ala Asn Arg Leu Ala Ala Thr Leu Arg Ala Ser Gly Ile 2835 2840 2845 Gly Arg Glu Ala Ile Val Gly Ile Leu Ala Glu Arg Ser Val Asp Leu 2850 2855 2860 Leu Val Ala Val Leu Ala Val Trp Lys Val Gly Gly Ala Tyr Val Pro 2865 2870 2875 2880 Leu Asp Pro Asp Tyr Pro Ala Glu Arg Val Arg Phe Met Leu Glu Asp 2885 2890 2895 Ser Gly Ala Lys Val Leu Leu Thr Gln Thr Pro Leu Arg Glu Arg Ala 2900 2905 2910 Glu Ala Trp Leu Gly Glu Glu Glu Leu Ala Leu Ala Ala Val Leu Tyr 2915 2920 2925 Leu Asp Asp Glu Ala Ser Tyr Ser Asp Glu Arg Ala Asn Ala Ser Val 2930 2935 2940 Val Ser Gly Lys Leu Thr Asp Ala Val Asp Asp Gly Asp Glu Ser His 2945 2950 2955 2960 Gln Asn Val Gly Thr Asp Ser Phe His Glu Ala Arg Pro Glu Asp Leu 2965 2970 2975 Ala Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly Lys Pro Lys Gly Val 2980 2985 2990 Met Ile Glu His Arg Ser Leu Val Asn Thr Ala Ala Gly Tyr Arg Arg 2995 3000 3005 Glu Tyr Arg Leu Asp Gln Phe Ala Val Arg Leu Leu Gln Leu Ala Ser 3010 3015 3020 Phe Ser Phe Asp Val Phe Val Gly Asp Ile Ala Arg Thr Leu Tyr Asn 3025 3030 3035 3040 Gly Gly Thr Met Val Ile Val Pro Lys Asp Asp Arg Ile Asp Pro Ser 3045 3050 3055 Arg Leu His His Trp Ile Glu Arg Glu Arg Val Thr Ile Phe Glu Ser 3060 3065 3070 Thr Pro Ala Leu Ile Val Pro Phe Leu Glu Tyr Val Tyr Glu Gln Gln 3075 3080 3085 Leu Asp Met Ser Arg Leu Glu Leu Leu Ile Thr Ser Ser Asp Ser Cys 3090 3095 3100 Ser Val Ala Asp Tyr Arg Thr Leu Gln Glu Arg Phe Gly Ser Leu Phe 3105 3110 3115 3120 Arg Ile Ile Asn Ala Tyr Gly Val Thr Glu Ala Ala Ile Asp Ser Ser 3125 3130 3135 Phe Tyr Asp Glu Glu Leu Ala Lys Leu Pro Gln Thr Gly Asn Val Pro 3140 3145 3150 Ile Gly Lys Ala Trp Leu Asn Ala Lys Phe Tyr Ile Val Asp Ala His 3155 3160 3165 Leu Asn Pro Val Pro Val Gly Val Leu Gly Glu Leu Val Ile Gly Gly 3170 3175 3180 Val Gly Val Ala Arg Gly Tyr Leu Asn Arg Pro Glu Leu Thr Glu Glu 3185 3190 3195 3200 Lys Phe Val Asp Ser Pro Phe Ala Glu Gly Glu Arg Leu Tyr Arg Thr 3205 3210 3215 Gly Asp Leu Ala Arg Trp Met Glu Asp Gly Asn Val Asp Phe Ile Gly 3220 3225 3230 Arg Ile Asp Asn Gln Ala Lys Ile Arg Gly Tyr Arg Ile Glu Thr Gly 3235 3240 3245 Glu Ile Glu Ser Gln Leu Leu Arg Val Glu Gly Val Arg Glu Ala Val 3250 3255 3260 Val Leu Val Arg Ser Asp Ala Asn Gly Gln Lys Ala Leu Cys Ala Tyr 3265 3270 3275 3280 Tyr Thr Pro Asp Thr Gly Ala Glu Leu Ala Val Asn Asp Leu Arg Gly 3285 3290 3295 Ala Leu Ala Gln Glu Leu Pro Gly Tyr Met Ile Pro Ser Tyr Phe Val 3300 3305 3310 Glu Leu Glu Arg Leu Pro Leu Thr Pro Asn Gly Lys Ile Asp Arg Lys 3315 3320 3325 Ala Leu Pro Ala Pro Glu Gly Glu Ala Gly Ser Gly Thr Glu Tyr Val 3330 3335 3340 Ala Pro Arg Asn Glu Leu Glu Thr Lys Leu Ala Ala Ile Trp Gln Glu 3345 3350 3355 3360 Val Leu Gly Leu Ala Lys Glu Ile Gly Val His Asp Asn Phe Phe Asp 3365 3370 3375 Ile Gly Gly His Ser Leu Arg Ala Thr Thr Leu Val Ser Lys Ile His 3380 3385 3390 Lys Glu Leu Asn Val Asp Leu Pro Leu Arg Asp Val Phe Arg His Ser 3395 3400 3405 Thr Ile Glu Ser Met Ala Ala Ala Ile Ser Arg Leu Asp Glu Gln Thr 3410 3415 3420 Phe Val Ala Ile Pro Val Ala Asp Asp Arg Glu Val Tyr Pro Gln Ser 3425 3430 3435 3440 Phe Ala Gln Lys Arg Leu Phe Ile Leu Asn Gln Leu Glu Gly Ala Glu 3445 3450 3455 Leu Ser Tyr Asn Met Pro Glu Ala Met Leu Leu Glu Gly Ala Leu Asp 3460 3465 3470 Arg Ala Arg Phe Glu Glu Ala Phe Arg Lys Leu Val Ala Arg His Glu 3475 3480 3485 Thr Leu Arg Thr Gly Phe Glu Met Val Asp Gly Glu Ala Ser Gln Arg 3490 3495 3500 Ile Tyr Gln Asp Val Asn Phe Ala Val Glu Phe Tyr Arg Ala Asp Glu 3505 3510 3515 3520 Gln Glu Ala Glu Glu Thr Val Arg Arg Phe Val Arg Pro Phe Asp Leu 3525 3530 3535 Ala Lys Pro Pro Leu Leu Arg Val Gly Leu Val Glu Leu Ala Pro Glu 3540 3545 3550 Arg His Ile Leu Met Tyr Asp Met His His Ile Ile Ser Asp Gly Val 3555 3560 3565 Ser Met Glu Ile Phe Val Glu Glu Phe Val Arg Leu Tyr Gly Gly Glu 3570 3575 3580 Gln Leu Glu Pro Leu Arg Ile Gln Tyr Lys Asp Tyr Thr Val Trp Gln 3585 3590 3595 3600 His Ser Gln Glu Gln Lys Glu Arg Leu Gln Arg Gln Glu Ala Tyr Trp 3605 3610 3615 Leu Asp Met Phe Gln Gly Glu Leu Pro Val Leu Glu Met Pro Thr Asp 3620 3625 3630 Tyr Pro Arg Pro Ala Val Gln Ser Tyr Glu Gly Gln Thr Leu Glu Phe 3635 3640 3645 Phe Phe Asp Ala Ser Lys Thr Asp Gly Leu Lys Gln Leu Ala Ser Glu 3650 3655 3660 Thr Gly Thr Thr Leu Phe Met Val Leu Leu Ala Ala Tyr Asn Val Leu 3665 3670 3675 3680 Leu His Lys Tyr Ser Gly Gln Glu Asp Val Ile Val Gly Thr Pro Ile 3685 3690 3695 Ala Gly Arg Asn His Gly Asp Val Gln Pro Leu Ile Gly Ile Phe Leu 3700 3705 3710 Asn Thr Leu Ala Ile Arg Ser Tyr Pro Ala Ser Glu Lys Thr Phe Leu 3715 3720 3725 Ser Tyr Leu Asn Glu Val Lys Glu Thr Thr Leu His Ala Phe Glu His 3730 3735 3740 Gln Asn Tyr Pro Phe Glu Glu Leu Val Asp Lys Val Gln Val Thr Arg 3745 3750 3755 3760 Asp Leu Ser Arg Asn Pro Leu Phe Asp Thr Leu Phe Thr Met Gln Asn 3765 3770 3775 Thr Glu Asn Glu Glu Phe Glu Leu Glu Gly Leu Arg Leu Ile Pro Tyr 3780 3785 3790 Pro Ser Ala Leu Asp Thr Ala Lys Phe Asp Ile Ser Leu Asp Val Gly 3795 3800 3805 Glu Glu Asn Gly Gly Leu Asp Tyr Ser Phe Glu Tyr Ala Thr Ala Leu 3810 3815 3820 Tyr Lys Arg Glu Thr Ile Glu Arg Leu Ala Lys His Tyr Glu Gln Leu 3825 3830 3835 3840 Leu Val Thr Ile Val Ser Arg Pro Asp Ala Lys Ile Ala Glu Leu Asn 3845 3850 3855 Leu Leu Thr Ala Glu Glu Lys Glu Gln Ile Leu Gly Ala Phe Asn Pro 3860 3865 3870 Ala Gln Pro Glu Ala Ala Pro Ala Ala Ala Phe His Arg Leu Phe Glu 3875 3880 3885 Glu Gln Ala Glu Arg Thr Pro Glu Ala Glu Ala Val Val Tyr Glu Asn 3890 3895 3900 Asp Arg Leu Thr Tyr Ala Glu Leu Asn Glu Arg Ala Asn Arg Leu Ala 3905 3910 3915 3920 Ala Thr Leu Arg Ala Ser Gly Ile Gly Arg Glu Thr Ile Val Gly Ile 3925 3930 3935 Leu Ala Glu Arg Ser Val Asp Leu Leu Val Ala Val Leu Ala Val Trp 3940 3945 3950 Lys Ala Gly Gly Ala Tyr Val Pro Leu Asp Pro Asp Tyr Pro Ala Glu 3955 3960 3965 Arg Val Arg Phe Met Leu Glu Asp Ser Gly Ala Lys Val Leu Leu Thr 3970 3975 3980 Gln Thr Pro Leu Arg Glu Arg Ala Glu Ala Trp Leu Gly Glu Glu Glu 3985 3990 3995 4000 Leu Ala Leu Ala Ala Val Leu Tyr Leu Asp Asp Glu Ala Ser Tyr Ser 4005 4010 4015 Glu Glu Arg Ala Asn Ala Ser Val Val Ser Gly Lys Leu Thr Asp Ala 4020 4025 4030 Val Asp Asp Gly Asp Glu Ser His Gln Asn Val Gly Thr Asp Ser Phe 4035 4040 4045 His Glu Ala Arg Pro Glu Asp Leu Ala Tyr Val Ile Tyr Thr Ser Gly 4050 4055 4060 Thr Thr Gly Lys Pro Lys Gly Val Met Ile Glu His Arg Ser Leu Val 4065 4070 4075 4080 Asn Thr Ala Ala Gly Tyr Arg Arg Glu Tyr Arg Leu Asp Gln Phe Pro 4085 4090 4095 Val Arg Leu Leu Gln Leu Ala Ser Phe Ser Phe Asp Val Phe Val Gly 4100 4105 4110 Asp Ile Ala Arg Thr Leu Tyr Asn Gly Gly Thr Met Val Ile Val Pro 4115 4120 4125 Lys Asp Asp Arg Ile Asp Pro Ser Arg Leu His His Trp Ile Glu Arg 4130 4135 4140 Glu Arg Val Thr Ile Phe Glu Ser Thr Pro Ala Leu Ile Val Pro Phe 4145 4150 4155 4160 Leu Asp Tyr Val His Glu Gln Gln Leu Asp Met Ser Trp Met Glu Leu 4165 4170 4175 Leu Ile Thr Ser Ser Asp Ser Cys Ser Val Ala Asp Tyr Arg Thr Leu 4180 4185 4190 Gln Glu Arg Phe Gly Ser Leu Phe Arg Ile Ile Asn Ala Tyr Gly Val 4195 4200 4205 Thr Glu Ala Ala Ile Asp Ser Ser Phe Tyr Asp Glu Pro Leu Thr Lys 4210 4215 4220 Leu Pro Gln Thr Gly Asn Val Pro Ile Gly Lys Ala Trp Leu Asn Ala 4225 4230 4235 4240 Lys Phe Tyr Ile Val Asp Ala His Leu Asn Pro Val Pro Val Gly Val 4245 4250 4255 Leu Gly Glu Leu Val Ile Gly Gly Val Gly Val Ala Arg Gly Tyr Leu 4260 4265 4270 Asn Arg Pro Glu Leu Thr Glu Glu Lys Phe Val Asp Ser Pro Phe Ala 4275 4280 4285 Ala Gly Glu Arg Leu Tyr Arg Thr Gly Asp Leu Ala Arg Trp Met Glu 4290 4295 4300 Asp Gly Asn Val Asp Phe Ile Gly Arg Ile Asp Asn Gln Thr Lys Ile 4305 4310 4315 4320 Arg Gly Tyr Arg Ile Glu Thr Gly Glu Ile Glu Ser Gln Leu Leu Arg 4325 4330 4335 Val Glu Gly Val Arg Glu Ala Val Val Leu Val Arg Ser Asp Ala Asn 4340 4345 4350 Gly Gln Lys Ala Leu Cys Ala Tyr Tyr Thr Pro Asp Thr Gly Ala Glu 4355 4360 4365 Leu Ala Val Asn Asp Leu Arg Ser Ala Leu Ala Gln Glu Leu Pro Gly 4370 4375 4380 Tyr Met Ile Pro Ser His Phe Val Glu Thr Glu Arg Leu Pro Leu Thr 4385 4390 4395 4400 Pro Asn Gly Lys Ile Asp Arg Lys Ala Leu Pro Ala Pro Glu Gly Glu 4405 4410 4415 Ala Gly Ser Gly Thr Glu Tyr Val Ala Pro Arg Asn Glu Leu Glu Thr 4420 4425 4430 Lys Leu Ala Ala Ile Trp Gln Glu Val Leu Gly Leu Ala Lys Glu Ile 4435 4440 4445 Gly Val His Asp Asn Phe Phe Asp Ile Gly Gly His Ser Leu Arg Ala 4450 4455 4460 Thr Thr Leu Ala Gly Lys Val Phe Lys Glu Leu Asn Val Asn Leu Pro 4465 4470 4475 4480 Leu Arg Asp Val Phe Arg His Ser Thr Ile Ala Ala Met Ala Glu Ala 4485 4490 4495 Ile Ala Arg Met Glu Arg Arg Glu His Glu Ala Ile Pro Gln Ala Glu 4500 4505 4510 Glu Arg Glu Tyr Tyr Pro Leu Ser Ser Ala Gln Lys Arg Leu Phe Ile 4515 4520 4525 Gln His Thr Leu Asp Gly Ala Asp Gln Leu Tyr Asn Met Pro Glu Leu 4530 4535 4540 Val Gln Val Glu Gly Glu Phe Asp Leu Asp Arg Leu Glu Ala Ala Leu 4545 4550 4555 4560 Arg Lys Leu Ile Thr Arg His Glu Ser Leu Arg Thr Gly Phe Glu Ile 4565 4570 4575 Val Lys Ala Glu Ala Val Gln Arg Ile Tyr Pro Gln Val Asp Phe Ala 4580 4585 4590 Ile Glu His His Gln Ala Asp Lys Glu Asp Ala Ala Gln Ile Glu Gln 4595 4600 4605 Ile Val Arg Ser Phe Val Arg Pro Phe Asp Leu Gly Lys Pro Pro Leu 4610 4615 4620 Leu Arg Ala Gly Val Ile Glu Leu Glu Pro Asn Leu His Ile Leu Leu 4625 4630 4635 4640 Phe Asp Met His His Met Val Ser Asp Gly Val Ser Met Ala Ile Val 4645 4650 4655 Ile Asp Glu Phe Ser Ser Phe Tyr Ala Gly Glu Glu Leu Pro Pro Leu 4660 4665 4670 Arg Ile Gln Tyr Lys Asp Tyr Ala Val Trp Gln Gln Ser Lys Ala His 4675 4680 4685 Arg Glu Arg Ile Gly Arg Gln Glu Ala Tyr Trp Leu Gln Thr Phe Glu 4690 4695 4700 Gly Glu Leu Pro Thr Ala Asp Leu Pro Met Asp Tyr Glu Arg Ser Ala 4705 4710 4715 4720 Ala Arg Ser Tyr Glu Gly Ala His Leu Glu Phe Asp Val Glu Ala Ser 4725 4730 4735 Leu Ser Ala Arg Leu Arg Glu Leu Ala Ala Glu Arg Glu Ser Thr Leu 4740 4745 4750 Phe Met Val Leu Leu Ala Ala Tyr Thr Val Leu Leu Ser Lys Tyr Ser 4755 4760 4765 Gly Gln Glu Asp Leu Val Val Gly Thr Pro Val Ala Gly Arg Thr Asn 4770 4775 4780 Ala Asp Leu Glu Pro Val Ile Gly Met Phe Val Asn Thr Leu Ala Ile 4785 4790 4795 4800 Arg Asn Arg Pro Ser Gly Asp Lys Thr Phe Leu Ser Tyr Leu Glu Glu 4805 4810 4815 Val Lys Glu Thr Ala Leu Gly Ala Phe Glu Asn Gln Asp Tyr Pro Phe 4820 4825 4830 Glu Glu Leu Val Glu Arg Leu Asn Val Lys Arg Glu Pro Gly Arg Phe 4835 4840 4845 Pro Leu Phe Asp Ala Val Phe Asp Leu Gln Asn Ile Glu Glu Arg Asp 4850 4855 4860 Ala Glu Leu Glu Gly Val Ser Leu Lys Thr Tyr Glu Leu Asp His Leu 4865 4870 4875 4880 Glu Glu Ala Lys Phe Asp Leu Thr Leu Phe Met Tyr Glu Asn Asn Gly 4885 4890 4895 Ala Leu Ser Gly Gly Phe Phe Tyr Ala Thr Lys Leu Phe Lys Glu Ala 4900 4905 4910 Met Ile Arg Thr Leu Thr Glu Asp Tyr Leu Arg Val Leu Ser Gln Ile 4915 4920 4925 Ala Glu Asn Pro Gln Leu Glu Leu Ser Arg Ile Glu Cys His Lys Pro 4930 4935 4940 Ala Ala Gly Ala Lys Ser Ala Val Asp Thr Ile Glu Phe Ala Phe 4945 4950 4955 <210> 2 <211> 1102 <212> PRT <213> Paenibacillus polymyxa F4 <400> 2 Met Lys Ser Leu Phe Glu Lys Glu Glu Arg Tyr Trp Ser Gly Lys Phe 1 5 10 15 Asp Ala Asp Asp Ser Leu Ser Phe Leu Pro Tyr Ser Gln Ser Ser Lys 20 25 30 Leu Ser Ala Asp Gly Glu Ala Ala Ala Glu Pro Gly Leu Leu His Arg 35 40 45 Thr Leu Pro Ser Glu Leu Ser Glu Arg Ile Ile Cys Leu Ala Asn Gly 50 55 60 Ser Asp Leu Ala Leu Tyr Met Ile Val Leu Ala Gly Val Lys Ser Leu 65 70 75 80 Leu Phe Lys Tyr Thr Gly Arg Asp Gln Val Leu Val Gly Met Pro Ser 85 90 95 Tyr Ser Ala Asp Pro Asp Gly Thr Pro Pro Pro His Asp Ile Leu Val 100 105 110 Ile Lys Thr Ser Val Ser Arg Gln Thr Thr Leu Lys Thr Leu Leu Gly 115 120 125 Gly Ile Lys Ala Ser Ile Gly Glu Ala Leu Glu His Gln His Leu Pro 130 135 140 Phe Arg Lys Met Val Glu Pro Leu His Leu Asp Tyr Thr Gly Asp Gly 145 150 155 160 Leu Pro Val Val Asn Thr Val Val Ser Phe Ala Pro Ile His Pro Glu 165 170 175 Pro Leu Gly Asn Arg Val Ala Ala Asp Thr Val Phe Arg Phe Asp Arg 180 185 190 Gln Asn His Ser Ile Glu Leu Glu Ile Ser Phe Asp Gly Gln Arg Tyr 195 200 205 Glu Arg Ala Phe Val Glu Gln Ala Ala Asp His Leu Val Arg Leu Leu 210 215 220 Ser Val Leu Leu Phe Gln Pro Asp Leu Glu Leu Gly Gln Ala Asp Val 225 230 235 240 Leu Ser Pro Asp Glu Arg Glu Thr Leu Leu Lys Arg Phe Asn Asp Thr 245 250 255 Glu Thr Glu Phe Glu Arg Gly Lys Thr Ile Tyr Gly Leu Phe Glu Glu 260 265 270 Gln Ala Glu Leu Tyr Pro Asp Asn Val Ala Ala Val Met Asn Glu Arg 275 280 285 Gln Leu Thr Tyr Arg Glu Leu Asn Glu Arg Ser Asn Arg Leu Ala Arg 290 295 300 Lys Leu Arg Glu Thr Gly Val Glu Ala Asp Gln Leu Val Ala Ile Leu 305 310 315 320 Ala Glu Arg Ser Leu Asp Met Val Val Gly Ile Leu Ala Ile Leu Lys 325 330 335 Ala Gly Gly Ala Tyr Val Pro Val Asp Pro Asp Tyr Pro Glu Glu Arg 340 345 350 Ile Arg Phe Met Ile Glu Asp Ser Gly Ala Pro Leu Leu Leu Ile Gln 355 360 365 Lys His Leu His Glu Lys Thr Asp Phe Ala Gly Thr Arg Leu Glu Leu 370 375 380 Asp Asp Phe Val Trp Gly Asp Arg Gly Ala Asp Ser Ala Asp Val Arg 385 390 395 400 Asp Ala Ser Asn Leu Glu Pro Ile Ser Gly Pro Gly Asn Leu Ala Tyr 405 410 415 Val Ile Tyr Thr Ser Gly Thr Thr Gly Arg Pro Lys Gly Thr Leu Ile 420 425 430 Glu His Lys Asn Val Val Arg Leu Leu Phe Asn Asp Lys Asn Leu Phe 435 440 445 Asp Phe Gly Pro Ser Asp Thr Trp Thr Leu Phe His Ser Phe Cys Phe 450 455 460 Asp Phe Ser Val Trp Glu Met Tyr Gly Ala Leu Leu Tyr Gly Gly Lys 465 470 475 480 Leu Val Ile Val Pro Pro Leu Thr Ala Lys Asn Pro Ala Asp Phe Leu 485 490 495 Ala Leu Leu Gly Arg Glu Gln Val Thr Ile Leu Asn Gln Thr Pro Thr 500 505 510 Tyr Phe Tyr Gln Leu Leu Arg Lys Val Leu Ala Asp His Pro Tyr Asp 515 520 525 Leu Arg Ile Arg Asn Val Ile Phe Gly Gly Glu Ala Leu Ser Pro Leu 530 535 540 Leu Leu Lys Gly Phe Lys Thr Lys Tyr Pro Glu Thr Lys Leu Ile Asn 545 550 555 560 Met Tyr Gly Ile Thr Glu Thr Thr Val His Val Thr Tyr Lys Glu Ile 565 570 575 Thr Trp Val Glu Met Glu Ala Ala Lys Ser Asn Ile Gly Lys Pro Ile 580 585 590 Pro Thr Leu Ser Val Tyr Val Leu Asp Glu Asn Arg Ser Pro Val Pro 595 600 605 Ile Gly Val Ala Gly Glu Met Tyr Val Ala Gly Glu Gly Leu Ala Arg 610 615 620 Gly Tyr Leu Asn Arg Pro Asp Leu Thr Ala Glu Lys Phe Val Asp Ser 625 630 635 640 Pro Phe Ala Glu Gly Glu Lys Leu Tyr Arg Ser Gly Asp Leu Ala Ala 645 650 655 Trp Leu Pro Asp Gly Asn Ile Glu Tyr Leu Gly Arg Ile Asp His Gln 660 665 670 Val Lys Ile Arg Gly Tyr Arg Ile Glu Leu Asp Glu Ile Glu Thr Gln 675 680 685 Leu Leu Lys Ile Ala Ala Val Gln Glu Ala Lys Val Leu Asp Arg Asp 690 695 700 Asp Ala Asn Gly His Lys Gln Leu Val Ala Tyr Tyr Val Ala Glu Thr 705 710 715 720 Arg Leu Ala Ala His Glu Leu Lys Glu Glu Leu Ala Lys Gln Leu Pro 725 730 735 Gly Tyr Met Ile Pro Ser His Leu Val Gln Leu Ser Arg Met Pro Leu 740 745 750 Thr Pro Asn Gly Lys Ile Asp Arg Lys Ala Leu Pro Ala Pro Glu Glu 755 760 765 Ala Ala Ala Gly Gly Ala Glu Tyr Val Ala Pro Arg Thr Leu Leu Glu 770 775 780 Met Lys Ile Ala Arg Val Trp Gln Asp Thr Leu Gly Val Pro Gln Val 785 790 795 800 Gly Val Lys Asp Asn Phe Phe Glu Leu Gly Gly Asn Ser Leu Ser Leu 805 810 815 Met Arg Leu Val Gln Ala Val Tyr Asp Glu Thr Asp Ile Glu Ile Pro 820 825 830 Leu Asn Arg Gln Phe His Asn Leu Thr Val Glu Ala Met Ala Phe Gly 835 840 845 Glu Gly Asp Leu Gly Leu Asp Lys Gly Gly Asp Ser Phe Ile Lys Leu 850 855 860 Asn Lys Ala Gly Asp Leu Asn Val Phe Cys Phe Pro Pro Gly Ser Gly 865 870 875 880 Phe Gly Ile Gly Tyr Arg Glu Leu Ala Ser Arg Leu Asp Gly Arg Phe 885 890 895 Val Leu Tyr Gly Ile Asp Phe Ile Asp Asp Ala Val Asp Tyr Glu Ala 900 905 910 Met Leu Asn Arg Tyr Val Asp Glu Ile Val Arg Ile Gln Pro Glu Gly 915 920 925 Pro Tyr Val Leu Leu Gly Tyr Cys Phe Gly Gly Asn Leu Thr Phe Glu 930 935 940 Val Ala Lys Thr Met Glu Lys Arg Gly Tyr Ser Val Thr Asp Val Leu 945 950 955 960 Met Leu Asp Ser Trp Ile Lys Asp Thr Leu Thr Pro Ser Glu Thr Ser 965 970 975 Glu Lys Glu Leu Glu Glu Thr Leu Ala Asp Phe Asp Glu Glu Glu Lys 980 985 990 Glu Leu Met Ser Asn Pro Leu Val Arg Glu Arg Val His Arg Lys Val 995 1000 1005 Lys Ala Thr Leu Ala Tyr Glu Ala Gln Leu Ile Asn Ser Gly Thr Ile 1010 1015 1020 Pro Ala Arg Ile Tyr Glu Leu Ile Ala Lys Asp Ser Glu Ala Phe Arg 1025 1030 1035 1040 Leu Glu His Gln Leu Pro Ser Trp Arg Gly Ala Thr Thr Gln Ala Tyr 1045 1050 1055 Ala Asp Tyr Arg Leu Glu Gly Ala His Glu Glu Leu Leu Glu Leu Ala 1060 1065 1070 Arg Val Glu Glu Thr Ala Val Val Ile Arg Asp Ile Leu Glu Gln Val 1075 1080 1085 Lys Arg Arg Ile Glu Ala Glu Ala Gly Val Leu His Gly Ser 1090 1095 1100 <210> 3 <211> 6282 <212> PRT <213> Paenibacillus polymyxa F4 <400> 3 Met Arg Glu Asn Thr Asn Glu Gln Tyr Gly Leu Thr Gln Ala Gln Arg 1 5 10 15 Arg Ile Trp Phe Met Glu Ile Met Asn Pro Gly Thr Ser Ile Thr Met 20 25 30 Leu Ser Ala Thr Tyr Gln Ile Thr Gly Glu Ile Asn Thr Gln Leu Leu 35 40 45 Glu Gln Ala Ala Ala Glu Ile Val Lys Thr Tyr Asp Val Phe Arg Ile 50 55 60 Arg Ile Ser Gly Asp Leu Gln Asn Pro Thr Gln Trp Phe Glu Glu Pro 65 70 75 80 Glu Asn Val Gln Ala Arg Ile Ser Arg Leu Glu Ile Gly Thr Thr Glu 85 90 95 Gln Phe Tyr Ala Trp Val Lys Glu Val Ser Glu Lys Pro Ala Ser Val 100 105 110 Phe Asp Glu His Leu Tyr Gln Phe Thr Ile Ile His Phe Ala Asn Gly 115 120 125 Gln Val Trp Leu Asn Leu Thr Val Asn His Ile Ile Ala Asp Gly Leu 130 135 140 Ser Val Asn Ala Leu Leu His Ala Val Met Glu Lys Tyr Leu Glu Leu 145 150 155 160 Arg Lys Gly Ile Ser Ser Ser Tyr Gln Ala Pro Ser Tyr Leu Asp Tyr 165 170 175 Ile Ser Ala Glu Arg Glu Tyr Glu Gln Ser Gln Arg Tyr Gln Lys Gly 180 185 190 Lys Glu Tyr Trp Leu Thr Lys Tyr Asn Thr Leu Pro Glu Thr Thr Gly 195 200 205 Ile Lys Ser Tyr Pro Pro Phe Ser Ile Gly Ser Glu Ser Asn Lys Leu 210 215 220 Ala Ile Thr Leu Asp Gly Ser Arg Tyr Glu Arg Ile Leu Ala Phe Ser 225 230 235 240 Glu Gln Tyr Gln Val Ser Leu Tyr Thr Leu Phe Leu Ser Ala Met Tyr 245 250 255 Ala Leu Leu Tyr Lys Leu Thr Asp Ser Thr Asp Val Pro Val Gly Thr 260 265 270 Val Phe Ala Asn Arg Thr Ser Lys Lys Glu Lys Glu Thr Ile Gly Met 275 280 285 Phe Val Ser Thr Val Ala Thr Arg Ile Arg Leu Asn Pro Asp Arg His 290 295 300 Val Leu Ser Leu Ile Gln Thr Val Ser Lys Glu Asn Thr Ala Asp Leu 305 310 315 320 Arg Tyr Gln Lys Tyr Pro Tyr Asn Gln Leu Ile Gln Asp Leu Arg Glu 325 330 335 Gln His Gly Arg Asn Asp Leu Ser Gly Leu Phe Arg Thr Ser Leu Glu 340 345 350 Tyr Leu Pro Leu Lys Ile Val Glu Tyr Glu Glu Ile Lys Val Arg Leu 355 360 365 Glu Ala His Phe Ala Arg His Glu Met Asp Asp Leu Leu Leu Arg Phe 370 375 380 Asp His Met Leu Asn Glu Gly His Val Ile Leu His Ala Ser Tyr Arg 385 390 395 400 Thr Gly Leu Phe Glu Thr Ala Glu Ile Asp Arg Ile Met Glu Gln Tyr 405 410 415 Val Thr Val Leu Asp Gln Phe Leu Gln Thr Pro Glu Leu Pro Val Arg 420 425 430 Glu Ile Ser Leu Leu Ser Asp Glu Glu Arg His Arg Ile Leu Asn Val 435 440 445 Phe Asn Pro Pro Val Ala Gly Leu Ser Glu Gly Glu Ala Phe His Arg 450 455 460 Tyr Val Glu Lys Phe Ala Leu Glu Ile Pro Asp His Pro Ala Val Val 465 470 475 480 Tyr Met Asp Lys Gln Leu Thr Tyr Gly Glu Leu Asn Glu Arg Ala Glu 485 490 495 Arg Leu Ala Ser Leu Leu Arg Glu Gln Gly Val Gly Arg Glu Thr Ile 500 505 510 Thr Gly Ile Trp Ala Glu Arg Ser Val Glu Leu Leu Val Gly Val Leu 515 520 525 Ala Val Trp Lys Ala Gly Gly Ala Tyr Val Pro Leu Asp Pro Asp Tyr 530 535 540 Pro Ala Glu Arg Ile Glu Tyr Met Leu Ser Asp Ser Glu Ala Thr Val 545 550 555 560 Leu Leu Thr Gln Arg His Leu Leu Glu Arg Ala Gly Val Trp Leu Ala 565 570 575 Asp Asp Arg Leu Lys Leu Gln Ala Val Tyr Ala Met Asp Asp Glu Gln 580 585 590 Ile Tyr Asn Gly Asp Ala Leu Ala Val Glu Phe Glu Ser Ala Gly Ser 595 600 605 Ala Pro Gln Asp Leu Ala Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly 610 615 620 Arg Pro Lys Gly Val Met Ile Glu His Gly Ser Leu Val Asn Thr Ala 625 630 635 640 Asp Ala Tyr Arg Arg Glu Tyr Arg Leu Asp Gln Phe Pro Val Arg Leu 645 650 655 Leu Gln Leu Ala Ser Phe Ser Phe Asp Val Phe Val Gly Asp Ile Ala 660 665 670 Arg Thr Leu Tyr Asn Gly Gly Thr Met Val Ile Val Pro Lys Asp Asp 675 680 685 Arg Ile Asp Pro Asn Leu Leu Tyr Gly Trp Ile Arg Asp Gln Asn Ile 690 695 700 Thr Val Phe Glu Ser Thr Pro Ala Leu Ile Leu Pro Phe Met Gln His 705 710 715 720 Ile Tyr Glu Glu Gly Leu Asp Val Ser Ser Ile Gln Leu Leu Ile Thr 725 730 735 Ser Ser Asp Ala Cys Ser Val Thr Asp Tyr Arg Leu Leu Leu Glu Arg 740 745 750 Phe Gly Gly Gln Phe Arg Ile Ile Asn Ser Tyr Gly Val Thr Glu Ala 755 760 765 Ala Ile Asp Ser Ser Phe Tyr Gly Glu Pro Leu Asp Lys Leu Pro Pro 770 775 780 Ser Gly His Val Pro Ile Gly Lys Ala Trp Leu Asn Ala Arg Phe Tyr 785 790 795 800 Ile Val Asp Ala Ala Leu Lys Pro Val Pro Val Gly Val Pro Gly Glu 805 810 815 Leu Val Ile Gly Gly Ala Gly Val Ala Arg Gly Tyr Trp Asn Arg Leu 820 825 830 Asp Leu Thr Ala Glu Lys Phe Val Asp Ser Pro Phe Val Pro Gly Glu 835 840 845 Arg Val Tyr Arg Thr Gly Asp Leu Ala Arg Trp Leu Glu Asp Gly Asn 850 855 860 Val Asp Phe Ile Gly Arg Ile Asp Tyr Gln Val Lys Ile Arg Gly Phe 865 870 875 880 Arg Ile Glu Leu Gly Glu Ile Glu Thr Ala Leu Leu Arg Phe Pro Gly 885 890 895 Val Lys Gln Ala Val Val Thr Asp Arg Thr Asp Glu Gln Gly Gln Lys 900 905 910 Tyr Leu Cys Gly Tyr Val Ala Ala Asp Ala Ser Leu Gln Leu Ser Asp 915 920 925 Leu Leu Ser Gln Leu Lys Gln Glu Leu Pro Ala His Met Val Pro Ala 930 935 940 Arg Leu Val Ser Leu Asp Lys Leu Pro Leu Thr Pro Asn Gly Lys Ile 945 950 955 960 Asp Arg Lys Ala Leu Pro Glu Pro Thr Gly Glu Val Glu Thr Gly Gly 965 970 975 Glu Tyr Val Ala Pro Arg Thr Thr Leu Glu Thr Arg Leu Ala Leu Ile 980 985 990 Trp Gln Gln Val Leu Gly Ile Ala Arg Val Gly Val Gln Asp Asp Phe 995 1000 1005 Phe Asp Leu Gly Gly His Ser Leu Arg Ala Ser Thr Leu Val Ser Lys 1010 1015 1020 Ile Arg Lys Glu Leu Gln Val Glu Val Pro Leu Arg Glu Val Phe Arg 1025 1030 1035 1040 Tyr Thr Thr Ile Glu Gln Leu Ala Gln Arg Ile Gly Gly Leu Arg Gln 1045 1050 1055 Gln Glu Thr Tyr Glu Ile Thr Lys Ala Ala Glu Ala Glu Tyr Tyr Pro 1060 1065 1070 Val Ser Ser Glu Gln Lys Arg Leu Tyr Val Leu Arg Gln Leu Asp Gly 1075 1080 1085 Ala Glu Arg Ser Tyr Asn Met Ser Ala Ala Leu Leu Leu Glu Gly Lys 1090 1095 1100 Leu Asp Arg Thr Arg Val Glu His Ala Phe Arg Ala Leu Ile Gln Arg 1105 1110 1115 1120 His Glu Thr Leu Arg Thr Gly Ile Glu Gln Val Gln Gly Glu Leu Val 1125 1130 1135 Gln Arg Ile Tyr Asp Glu Val Glu Phe Ala Val Asp Tyr Phe Gln Ala 1140 1145 1150 Ser Glu Trp Glu Val Asp Gln Val Val Glu Ala Tyr Tyr His Pro Phe 1155 1160 1165 Asp Leu Thr Lys Pro Pro Leu Leu Arg Ile Gly Leu Ile Glu Val Asp 1170 1175 1180 Glu Asp Arg His Ile Leu Leu Phe Asp Met His His Ile Val Ser Asp 1185 1190 1195 1200 Gly Ile Ser Thr Ala Leu Leu Phe Asp Glu Phe Ser Arg Leu Tyr Arg 1205 1210 1215 Gly Glu Glu Leu Ala Pro Leu Arg Ile Gln Tyr Lys Asp Tyr Ala Val 1220 1225 1230 Trp Gln His Ser Glu Ala Tyr Gly Gln Met Leu Gln Pro Gln Lys Glu 1235 1240 1245 Tyr Trp Leu Lys Gln Leu Ser Gly Glu Leu Pro Val Leu Glu Leu Pro 1250 1255 1260 Thr Asp Phe Pro Arg Pro Ala Val Gln Ser Phe Asp Gly Arg Thr Val 1265 1270 1275 1280 Lys Phe Tyr Ile Glu Lys Asp Arg Thr Glu Lys Leu Lys Glu Leu Ala 1285 1290 1295 Ala Arg Thr Gly Thr Thr Leu Tyr Met Val Leu Leu Ser Ala Tyr Thr 1300 1305 1310 Ile Leu Met His Lys Tyr Ser Gly Gln Glu Asp Leu Ile Val Gly Thr 1315 1320 1325 Pro Ile Ala Gly Arg Thr Gln Glu Glu Val Gln Pro Ile Val Gly Met 1330 1335 1340 Phe Ile Asn Thr Leu Ala Ile Arg Ser Arg Pro Glu Arg Ser Lys Pro 1345 1350 1355 1360 Tyr Leu Ser Tyr Leu Glu Glu Ile Lys Asp Ile Thr Leu Gly Ala Phe 1365 1370 1375 Glu His Gln Asn Tyr Leu Phe Glu Asp Leu Val Glu Ser Leu His Ile 1380 1385 1390 Pro Arg Ala Thr Gly Arg Asn Pro Leu Phe Asp Thr Phe Phe Ser Leu 1395 1400 1405 Gln Asn Thr Glu Asn Glu Gln Ile Val Ile Glu Gly Leu Glu Gln Ser 1410 1415 1420 Phe Tyr Pro Leu Glu Asn Gln Thr Ser Lys Phe Glu Leu Leu Leu Asp 1425 1430 1435 1440 Ile Ser Glu Leu Asp Gly Gln Leu Glu Cys Arg Leu Glu Tyr Ala Thr 1445 1450 1455 Ala Leu Tyr Lys Gln Glu Thr Ala Glu Arg Phe Ala Arg His Tyr Asp 1460 1465 1470 Lys Leu Leu Glu Thr Ile Ala Ala Ala Pro Asp Gly Asp Ile Ala Ser 1475 1480 1485 Leu Glu Met Leu Thr Glu Glu Glu Ile Arg Glu Leu Val Arg Gly Phe 1490 1495 1500 Asn Asp Ser Glu Ala Asp Tyr Pro Arg Gln Gln Thr Ile His Gly Leu 1505 1510 1515 1520 Phe Glu Glu Gln Ala Glu Leu Tyr Pro Asp Asn Val Ala Ala Val Met 1525 1530 1535 Asn Glu Arg Gln Leu Thr Tyr Arg Glu Leu Asn Glu Arg Ser Asn Arg 1540 1545 1550 Leu Ala Arg Lys Leu Arg Glu Thr Gly Val Glu Ala Asp Gln Leu Val 1555 1560 1565 Ala Ile Leu Ala Glu Arg Ser Leu Asp Met Val Val Gly Ile Leu Ala 1570 1575 1580 Ile Leu Lys Ala Gly Gly Ala Tyr Val Pro Val Asp Pro Asp Tyr Pro 1585 1590 1595 1600 Glu Glu Arg Ile Arg Phe Met Ile Glu Asp Ser Gly Ala Pro Leu Leu 1605 1610 1615 Leu Ile Gln Lys His Leu His Glu Lys Thr Asp Phe Ala Gly Thr Arg 1620 1625 1630 Leu Glu Leu Asp Asp Phe Val Gly Gly Asp Arg Gly Ala Asn Ser Ala 1635 1640 1645 Asp Val Arg Asp Ala Ser Asn Leu Glu Pro Ile Ser Gly Pro Gly Asn 1650 1655 1660 Leu Ala Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly Arg Pro Lys Gly 1665 1670 1675 1680 Thr Leu Ile Glu His Lys Asn Val Val Arg Leu Leu Phe Asn Asp Lys 1685 1690 1695 Asn Leu Phe Asp Phe Gly Pro Ser Asp Thr Trp Thr Leu Phe His Ser 1700 1705 1710 Phe Cys Phe Asp Phe Ser Val Trp Glu Met Tyr Gly Ala Leu Leu Tyr 1715 1720 1725 Gly Gly Lys Leu Val Ile Val Pro Pro Leu Thr Ala Lys Ser Pro Ala 1730 1735 1740 Asp Phe Leu Ala Leu Leu Gly Arg Glu Gln Val Thr Ile Leu Asn Gln 1745 1750 1755 1760 Thr Pro Thr Tyr Phe Tyr Gln Leu Leu Arg Glu Val Leu Thr Asp His 1765 1770 1775 Pro Tyr Asp Leu Arg Ile Arg Asn Val Ile Phe Gly Gly Glu Ala Leu 1780 1785 1790 Ser Pro Leu Leu Leu Lys Gly Phe Lys Thr Lys Tyr Pro Glu Thr Lys 1795 1800 1805 Leu Ile Asn Met Tyr Gly Ile Thr Glu Thr Thr Val His Val Thr Tyr 1810 1815 1820 Lys Glu Ile Thr Trp Val Glu Met Glu Ala Ala Lys Ser Asn Ile Gly 1825 1830 1835 1840 Lys Pro Ile Pro Thr Leu Ser Val Tyr Val Leu Asp Glu Asn Arg Arg 1845 1850 1855 Pro Val Pro Ile Gly Val Ala Gly Glu Met Tyr Val Ala Gly Glu Gly 1860 1865 1870 Leu Ala Arg Gly Tyr Leu Asn Arg Pro Asp Leu Thr Ala Glu Lys Phe 1875 1880 1885 Val Asp Ser Pro Phe Ala Glu Gly Glu Lys Leu Tyr Arg Ser Gly Asp 1890 1895 1900 Leu Ala Ala Trp Leu Pro Asp Gly Asn Ile Glu Tyr Leu Gly Arg Ile 1905 1910 1915 1920 Asp His Gln Val Lys Ile Arg Gly Tyr Arg Ile Glu Leu Asp Glu Ile 1925 1930 1935 Glu Thr Gln Leu Leu Asn Val Glu Gly Val Glu Glu Ala Val Val Leu 1940 1945 1950 Ala Arg Gln Asp Gly Gly Gly Glu Lys Ala Leu Val Ala Tyr Phe Val 1955 1960 1965 Ala Asp Arg Thr Leu Thr Val Ser Glu Met Arg Thr Ser Leu Ala Gln 1970 1975 1980 Gly Met Pro Gly Tyr Met Ile Pro Ser Tyr Phe Leu Gln Leu Glu Arg 1985 1990 1995 2000 Met Pro Leu Thr Ser Asn Gly Lys Val Asp Arg Lys Ala Leu Pro Glu 2005 2010 2015 Pro Gln Gly Gly Leu Gln Thr Gly Val Glu Tyr Val Ala Pro Arg Asn 2020 2025 2030 Arg Thr Glu Ser Gln Leu Val Lys Ile Trp Glu Glu Val Leu Gly Tyr 2035 2040 2045 Ser Gly Ile Gly Val Leu Asp Asn Phe Phe Glu Leu Gly Gly His Ser 2050 2055 2060 Leu Arg Ala Thr Asn Leu Val Ser Lys Ile Arg Lys Glu Met Asn Val 2065 2070 2075 2080 Glu Leu Pro Leu Arg Asp Val Phe Arg Tyr Thr Thr Val Glu Ser Met 2085 2090 2095 Ala Gly Ala Ile Ala Ser Leu Glu Glu Thr Gln His Ser Ser Ile Pro 2100 2105 2110 Lys Ala Glu Glu Lys Ala Tyr Tyr Pro Val Ser Ser Ala Gln Lys Arg 2115 2120 2125 Leu Tyr Val Leu His Gln Leu Asp Ser Ser Glu Leu Asn Tyr Asn Leu 2130 2135 2140 Pro Ser Ala Leu Gln Leu Glu Gly Ala Leu Asn Glu Ala Lys Val Glu 2145 2150 2155 2160 Lys Ala Leu Thr Thr Leu Val Ala Arg His Asp Met Leu Arg Thr Gly 2165 2170 2175 Phe Glu Ile Val Asn Gly Glu Pro Val Gln Arg Ile His Pro Leu Val 2180 2185 2190 Ala Phe Lys Val Glu Lys Leu Gln Ala Ser Glu Asp Gln Val Ala Ala 2195 2200 2205 Ile Leu Glu Gly Phe Ile Gln Pro Phe Asp Leu Thr Gln Pro Pro Leu 2210 2215 2220 Leu Arg Ala Leu Leu Ile Glu Leu Glu Lys Glu Lys Phe Leu Leu Ala 2225 2230 2235 2240 Leu Asp Ile His His Ile Gly Ser Asp Gly Leu Ser Met Asp Val Leu 2245 2250 2255 Leu Arg Glu Phe Val Arg Leu Tyr Asn Gly Glu Glu Leu Pro Glu Leu 2260 2265 2270 Arg Ile Gln Tyr Lys Asp Tyr Ala Val Trp Gln Gln Ser Glu Glu Gln 2275 2280 2285 Arg Gln Arg Ile Lys Arg Gln Glu Glu Tyr Trp Arg Gly Val Phe Ser 2290 2295 2300 Ser Glu Leu Pro Val Leu Glu Leu Pro Leu Asp Phe Ser Arg Pro Ala 2305 2310 2315 2320 Val Gln Gln Phe Asp Gly Gln Thr Leu Thr Phe Thr Leu Asp Ala Glu 2325 2330 2335 Lys Ser Glu Ala Leu Lys Arg Leu Ala Gly Asp Ser Gly Ala Thr Leu 2340 2345 2350 Tyr Met Leu Leu Leu Ala Ala Tyr Ser Val Leu Leu His Lys Tyr Ala 2355 2360 2365 Gly Gln Glu Asp Ile Val Val Gly Thr Pro Ile Ala Ala Arg Ser His 2370 2375 2380 Ala Asp Leu Gln Pro Ile Ile Gly Met Phe Val Asn Thr Leu Ala Leu 2385 2390 2395 2400 Arg Leu Gly Pro Val Ala Glu Arg Thr Phe Leu Asp Tyr Leu Gln Glu 2405 2410 2415 Val Lys Glu Thr Thr Leu Gly Ala Tyr Glu His Gln Asp Tyr Pro Phe 2420 2425 2430 Glu Glu Leu Val Glu Ala Leu Gln Val Ser Arg Asp Leu Ser Arg Asn 2435 2440 2445 Pro Leu Phe Asp Thr Met Phe Ser Leu Gln Lys His Glu Ser Leu Asp 2450 2455 2460 Leu Thr Leu Glu Gly Leu Gln Trp Ser Leu Phe Asp Ile Glu Glu Lys 2465 2470 2475 2480 Thr Ala Lys Phe Asp Leu Ser Phe Asp Ile Val Glu Ala Asp Asn Glu 2485 2490 2495 Leu Ile Cys Lys Ile Glu Tyr Ala Thr Ser Leu Phe Arg Gln Glu Thr 2500 2505 2510 Met Val Arg Leu Ala Gly His Tyr Glu Gln Leu Leu Ala Ser Ile Leu 2515 2520 2525 Ala Gln Pro Gly Ala Arg Ile Ser Asp Leu Asp Ile Leu Thr Asp Ser 2530 2535 2540 Glu Lys His Asp Leu Leu Val Gly Phe Asp Val Ser Ser Ser Ala Leu 2545 2550 2555 2560 Ala Lys Gln Pro Ala Ala Glu Gly Thr Gly Leu Glu Ala Asp Glu Ser 2565 2570 2575 Trp Arg Glu Arg Thr Phe His Glu Leu Phe Glu Glu Gln Ala Glu Arg 2580 2585 2590 Thr Pro Gly Ala Leu Ala Val Val Tyr Glu Asp Ser Lys Leu Thr Tyr 2595 2600 2605 Ala Glu Leu Asn Ala Lys Ala Asn Arg Leu Ala Tyr Ala Leu Arg Ala 2610 2615 2620 Arg Gly Val Lys Pro Glu Gln Val Val Gly Ile Leu Ala Gly Arg Ser 2625 2630 2635 2640 Ala Glu Leu Leu Ile Gly Val Leu Ala Val Trp Lys Ala Gly Gly Ala 2645 2650 2655 Tyr Val Pro Leu Asp Pro Asp Tyr Pro Ala Glu Arg Ile Glu Tyr Met 2660 2665 2670 Leu Ala Asp Ser Gly Ala Ser Val Leu Leu Thr Gln Thr Arg Leu Leu 2675 2680 2685 Glu Gln Ala Glu Ala Trp Arg Ser Asp Gly Ala Leu Val Leu Gln Thr 2690 2695 2700 Val Leu Ala Leu Asp Asp Ala Ala Thr Tyr Ser Leu Gly Ala Ala Glu 2705 2710 2715 2720 Ala Ala Val Gly Glu Ala Gly Ala Glu Ala Glu Ala Leu Ala Gln Ala 2725 2730 2735 Ala Ala Ala Glu Ser Ser Ala Thr Ala Glu Ala Glu Lys Asn Val Leu 2740 2745 2750 Ala Ala Asp Leu Ala Ser Asn Pro Ala Asn Val Asn Lys Pro Arg Asp 2755 2760 2765 Leu Ala Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly Arg Pro Lys Gly 2770 2775 2780 Val Ala Val Glu Asn Arg Ser Leu Val Asn Thr Ala Ala Gly Tyr Arg 2785 2790 2795 2800 Arg Glu Tyr Arg Leu Asp Gln Phe Pro Val Arg Leu Leu Gln Leu Ala 2805 2810 2815 Ser Phe Ser Phe Asp Val Phe Val Gly Asp Ile Ala Arg Thr Leu Tyr 2820 2825 2830 Asn Gly Gly Thr Met Val Ile Val Pro Lys Asp Asp Arg Ile Asp Pro 2835 2840 2845 Thr Arg Leu Tyr Gly Trp Ile Arg Asp Tyr Ala Val Thr Val Phe Glu 2850 2855 2860 Ser Thr Pro Ala Leu Ile Val Pro Phe Met Glu His Val His Ala Glu 2865 2870 2875 2880 Gly Leu Asp Leu Ser Ser Met Gln Leu Leu Ile Thr Ser Ser Asp Ala 2885 2890 2895 Cys Ser Val Ala Asp Tyr Arg Thr Leu Gln Glu Arg Phe Gly Ser Gln 2900 2905 2910 Phe Arg Ile Ile Asn Ser Tyr Gly Val Thr Glu Ala Ala Ile Asp Ser 2915 2920 2925 Ser Phe Tyr Asp Glu Pro Leu Glu Lys Leu Pro Lys Thr Gly Ser Val 2930 2935 2940 Pro Ile Gly Lys Ala Trp Leu Asn Ala Lys Phe Tyr Ile Val Asp Ala 2945 2950 2955 2960 Asn Leu Lys Pro Val Pro Ile Gly Val Leu Gly Glu Leu Val Ile Gly 2965 2970 2975 Gly Ala Gly Val Ala Arg Gly Tyr Leu Asn Arg Pro Asp Leu Thr Ala 2980 2985 2990 Glu Lys Phe Val Asp Ser Pro Phe Ala Ala Gly Glu Arg Leu Tyr Arg 2995 3000 3005 Thr Gly Asp Leu Ala Arg Trp Met Pro Asp Gly Asn Val Asp Phe Ile 3010 3015 3020 Gly Arg Ile Asp Asn Gln Val Lys Ile Arg Gly Tyr Arg Ile Glu Leu 3025 3030 3035 3040 Gly Glu Ile Glu Ala Ala Met Lys Asn Phe Ala Gly Val Arg Gln Ala 3045 3050 3055 Leu Val Ile Asp Arg Thr Asp Glu Arg Gly Gln Lys Tyr Leu Cys Gly 3060 3065 3070 Tyr Val Val Ala Asp Ser Ser Phe Asp Leu Glu Gly Leu Val Ala His 3075 3080 3085 Leu Asp Ala Ala Leu Pro Ser His Met Val Pro Ser Arg Ile Met Arg 3090 3095 3100 Leu Asp Gln Met Pro Leu Thr Pro Asn Gly Lys Ile Asp Arg Lys Ala 3105 3110 3115 3120 Leu Pro Val Pro Glu Gly Ser Ile Arg Ala Glu Ala Ala Tyr Thr Ala 3125 3130 3135 Pro Arg Thr Pro Ala Glu Gln Ala Leu Ala Ser Val Trp Gln Ser Val 3140 3145 3150 Leu Gly Val Asp Gln Val Gly Thr Met Asp Asn Phe Phe Ala Leu Gly 3155 3160 3165 Gly Asp Ser Ile Lys Ala Leu Gln Val Ser Ser Arg Leu Leu Gln Thr 3170 3175 3180 Gly Tyr Lys Leu Val Met Lys Asp Leu Phe His Tyr Pro Thr Ile Ser 3185 3190 3195 3200 Ala Leu Ser Leu Gln Leu Gln Thr Ala Glu Arg Thr Ala Ser Gln Ala 3205 3210 3215 Glu Val Thr Gly Glu Val Ile Leu Thr Pro Ile Gln Arg Trp Phe Phe 3220 3225 3230 Glu Gln Asn Pro Ala Asp Val His His Ser Asn Gln Ala Phe Met Gln 3235 3240 3245 Phe Ser Lys Gln Gly Phe Asp Glu Glu Ala Leu Arg Gln Ala Val Arg 3250 3255 3260 Gln Leu Val Val His His Asp Ala Leu Arg Thr Val Tyr Arg Gln Thr 3265 3270 3275 3280 Glu Asn Gly Tyr Thr Ala Trp Asn Arg Gly Ala Gly Glu Asn Glu Ala 3285 3290 3295 Leu Phe Asp Leu Glu Val Val Asp Phe Lys Gly Val Gly Asp Val Lys 3300 3305 3310 Glu Ala Val Glu Ala Lys Ala Asn Asp Ile Gln Ala Ser Ile Asp Leu 3315 3320 3325 Glu Asn Gly Pro Leu Val Lys Leu Gly Leu Phe Arg Cys Asp Asp Gly 3330 3335 3340 Asp His Leu Leu Ile Ala Ile His His Leu Val Val Asp Gly Val Ser 3345 3350 3355 3360 Trp Arg Ile Leu Leu Glu Asp Phe Ala Ala Cys Tyr Glu Gln Ala Leu 3365 3370 3375 Gln Gly Gln Pro Ile Arg Leu Pro Leu Lys Thr Asp Ser Phe Gln Thr 3380 3385 3390 Trp Ala Lys Gln Leu Ala Asp Tyr Ala Asn Gly Pro Ala Met Glu Ser 3395 3400 3405 Glu Arg Glu Tyr Trp Gln His Ile Glu Gln Leu Thr Tyr Glu Pro Leu 3410 3415 3420 Pro Lys Asp Phe Glu Gln Gly Arg Ser Lys Leu Lys Asp Ser Gly Leu 3425 3430 3435 3440 Val Thr Val Arg Trp Thr Ala Glu Glu Thr Glu Gln Leu Leu Lys Gln 3445 3450 3455 Ala His Cys Ala Tyr His Thr Glu Met Asn Asp Leu Leu Leu Ala Ala 3460 3465 3470 Leu Gly Leu Ala Val Gln Ala Trp Ser Gly Arg Glu Arg Val Leu Val 3475 3480 3485 Asn Leu Glu Gly His Gly Arg Glu Asp Ile Leu Pro Asn Val Asp Ile 3490 3495 3500 Thr Arg Thr Val Gly Trp Phe Thr Ser Gln Phe Pro Val Val Leu Glu 3505 3510 3515 3520 Pro Gly His Ala Gln Ala Leu Gly His Gln Val Lys Gln Val Lys Glu 3525 3530 3535 Ser Leu Arg Arg Ile Pro Asn Lys Gly Ile Gly Tyr Gly Ile Leu Arg 3540 3545 3550 Tyr Leu Ser Ala Pro Arg Asp Gly Glu Arg Phe Ala Leu Glu Pro Glu 3555 3560 3565 Ile Ser Phe Asn Tyr Leu Gly Gln Phe Asp Gln Asp Tyr Glu Ser Ser 3570 3575 3580 Gly Ser Arg Pro Ser Pro Phe Ser Pro Gly Ser Asp Ser Ser Pro Asp 3585 3590 3595 3600 Ala Val Met Asp Tyr Val Leu Asp Ile Asn Gly Met Val Ser Glu Gly 3605 3610 3615 Val Gln Glu Leu Thr Ile Arg Tyr Gly Glu Thr Gln Tyr Lys Arg Glu 3620 3625 3630 Thr Val Glu Arg Leu Gly Thr Leu Leu His Ser Ser Leu Arg Glu Val 3635 3640 3645 Ile Ser His Cys Val Ser Lys Glu Arg Pro Glu Leu Thr Pro Ser Asp 3650 3655 3660 Val Leu Leu Gln Asp Val Thr Leu Glu Glu Leu Glu Arg Leu Ala Glu 3665 3670 3675 3680 His Thr Ala Ala Leu Gly Glu Leu Glu Asn Val Tyr Thr Leu Thr Pro 3685 3690 3695 Leu Gln Lys Gly Met Leu Phe His Ser Leu Leu Asp Ala Asp Ser Glu 3700 3705 3710 Ala Tyr Phe Glu Gln Val Thr Phe Asp Leu Tyr Gly Ser Leu Asn Val 3715 3720 3725 Glu Ala Phe Thr Gln Gly Leu Asp Thr Leu Val Gln Arg Asn Glu Ala 3730 3735 3740 Leu Arg Thr Asn Phe Ile Thr Gly Trp Arg Asp Glu Pro Ile Gln Val 3745 3750 3755 3760 Val Phe Arg Glu Arg Lys Cys Glu Val Tyr Phe Glu Asp Ile Arg Ser 3765 3770 3775 Val Ser Asp Glu Asp Pro Glu Lys Thr Ile Ala Asp Phe Val Ser Ala 3780 3785 3790 Asp Lys Ala Asn Lys Phe Asp Leu Ala Arg Gly Ser Leu Met Arg Val 3795 3800 3805 Thr Val Leu Arg Thr Gly Asp Glu Ser Tyr His Val Ile Trp Ser His 3810 3815 3820 His His Ile Leu Met Asp Gly Trp Cys Met Ser Phe Met Ile Lys Glu 3825 3830 3835 3840 Val Phe Asp Thr Tyr Phe Ala Phe Gln Glu Lys Arg Thr Leu Glu Leu 3845 3850 3855 Pro Pro Val Thr Ser Tyr Ser Arg Tyr Ile Glu Trp Leu Glu Ala Gln 3860 3865 3870 Asp Ala Ala Lys Ala Ser Arg Tyr Trp Ser Glu Tyr Leu Ala Gly Tyr 3875 3880 3885 Asp Gln Gln Thr Lys Leu Pro Gln Glu Lys Thr Gln Leu Lys Gln Gly 3890 3895 3900 Ala Phe Glu Ala Ala Glu Ile Asp Val Glu Leu Ser Lys Glu Leu Thr 3905 3910 3915 3920 Gly Gln Ile Glu Arg Val Ala Arg Gln Gln Gln Val Thr Leu Asn Thr 3925 3930 3935 Phe Met Gln Thr Val Trp Gly Leu Val Leu Gln Val Tyr Asn Asn Ser 3940 3945 3950 Glu Asp Val Val Phe Gly Ser Val Val Ser Gly Arg Pro Ala Glu Ile 3955 3960 3965 Pro Gly Ile Glu Ser Met Ile Gly Leu Phe Ile Asn Thr Ile Pro Val 3970 3975 3980 Arg Ile Gln Gly Lys Ala Glu Glu Thr Val Ala Asp Ile Leu Arg Lys 3985 3990 3995 4000 Thr Gln Asp Gln Ala Leu Ala Ser Gly Ala Tyr Glu Thr Phe Pro Leu 4005 4010 4015 Phe Glu Ile Gln Ser Leu Ser Glu His Lys Arg Asp Leu Ile Asn His 4020 4025 4030 Ile Met Val Phe Glu Asn Tyr Pro Met Glu Glu Gln Ile Glu Gln Val 4035 4040 4045 Val Gly Gly Asp Lys Glu Ala Leu Lys Ile Ala Asn Ile Gln Ser Pro 4050 4055 4060 Glu Gln Thr Asn Tyr Asp Leu Asp Ile Thr Val Ile Pro Glu Glu His 4065 4070 4075 4080 Ile Leu Leu Arg Phe Thr Tyr Asn Ala Leu Thr Phe Arg Glu Glu Asp 4085 4090 4095 Ile Arg Leu Ile His Gly His Phe Ala Arg Val Leu Glu Lys Val Ala 4100 4105 4110 Ala Asn Pro Ser Ile Arg Val Asn Gln Leu Glu Leu Leu Thr Ala Ala 4115 4120 4125 Glu Lys Glu Gln Ile Leu Gly Ala Phe Asn Pro Ala Gln Pro Glu Ala 4130 4135 4140 Ala Pro Ala Ala Ala Phe His Arg Leu Phe Glu Glu Gln Ala Glu Arg 4145 4150 4155 4160 Thr Pro Glu Ala Ala Ala Val Val Tyr Glu Asn Asp Arg Leu Thr Tyr 4165 4170 4175 Glu Glu Leu Asn Glu Arg Ala Asn Arg Leu Ala Ala Thr Leu Arg Ala 4180 4185 4190 Ser Gly Ile Gly Arg Glu Thr Ile Val Gly Ile Leu Ala Glu Arg Ser 4195 4200 4205 Val Asp Leu Leu Val Ala Val Leu Ala Val Trp Lys Ala Gly Gly Ala 4210 4215 4220 Tyr Val Pro Leu Asp Pro Asp Tyr Pro Ala Glu Arg Val Arg Phe Met 4225 4230 4235 4240 Leu Glu Asp Ser Gly Ala Lys Val Leu Leu Thr Gln Thr Pro Leu Arg 4245 4250 4255 Glu Arg Ala Glu Ala Trp Leu Gly Glu Glu Glu Leu Ala Leu Ala Ala 4260 4265 4270 Val Leu Tyr Leu Asp Asp Glu Thr Ser Tyr Ser Glu Glu Arg Thr Asn 4275 4280 4285 Ala Asp Ser Phe His Glu Ala Arg Pro Glu Asp Leu Ala Tyr Val Ile 4290 4295 4300 Tyr Thr Ser Gly Thr Thr Gly Lys Pro Lys Gly Val Met Ile Glu His 4305 4310 4315 4320 Arg Ser Leu Val Asn Thr Ala Ala Gly Tyr Arg Arg Glu Tyr Arg Leu 4325 4330 4335 Asp Gln Phe Pro Val Arg Leu Leu Gln Leu Ala Ser Phe Ser Phe Asp 4340 4345 4350 Val Phe Val Gly Asp Ile Ala Arg Thr Leu Tyr Asn Gly Gly Thr Met 4355 4360 4365 Val Ile Val Pro Lys Asp Asp Arg Ile Asp Pro Ser Arg Leu His His 4370 4375 4380 Trp Met Glu Arg Glu Arg Val Thr Ile Phe Glu Ser Thr Pro Ala Leu 4385 4390 4395 4400 Ile Val Pro Phe Leu Glu Tyr Val His Asp Gln Arg Leu Asp Met Ser 4405 4410 4415 Trp Met Glu Leu Leu Ile Thr Ser Ser Asp Ser Cys Ser Val Ala Asp 4420 4425 4430 Tyr Arg Thr Leu Gln Glu Arg Phe Gly Ser Leu Phe Arg Ile Ile Asn 4435 4440 4445 Ala Tyr Gly Val Thr Glu Ala Ala Ile Asp Ser Ser Phe Tyr Asp Glu 4450 4455 4460 Glu Leu Ala Lys Leu Pro Gln Thr Gly Asn Val Pro Ile Gly Lys Ala 4465 4470 4475 4480 Trp Leu Asn Ala Lys Phe Tyr Ile Val Asp Ala His Leu Asn Pro Val 4485 4490 4495 Pro Val Gly Val Leu Gly Glu Leu Val Ile Gly Gly Val Gly Val Ala 4500 4505 4510 Arg Gly Tyr Leu Asn Arg Pro Glu Leu Thr Glu Glu Lys Phe Val Asp 4515 4520 4525 Ser Pro Phe Ala Ala Gly Glu Arg Leu Tyr Arg Thr Gly Asp Leu Ala 4530 4535 4540 Arg Trp Met Glu Asp Gly Asn Val Asp Phe Ile Gly Arg Ile Asp Asn 4545 4550 4555 4560 Gln Ala Lys Ile Arg Gly Tyr Arg Ile Glu Thr Gly Glu Val Glu Ala 4565 4570 4575 Lys Leu Leu Ser Val Gly Gly Val Lys Glu Ala Val Val Val Val Arg 4580 4585 4590 Glu Asp Gln Glu Gly Gln Lys Ala Leu Cys Ala Tyr Tyr Thr Ala Glu 4595 4600 4605 Glu Gly Leu Thr Ala Ala Asp Leu Lys Arg Ala Ile Ala Asn Glu Leu 4610 4615 4620 Pro Gly Tyr Met Ile Pro Ser Tyr Phe Val Glu Leu Glu His Leu Pro 4625 4630 4635 4640 Leu Thr Pro Asn Gly Lys Ile Asp Arg Lys Ala Leu Pro Ala Pro Glu 4645 4650 4655 Gly Gly Ala Ser Ala Gly Arg Glu Tyr Val Ala Pro Arg Thr Glu Leu 4660 4665 4670 Glu Ala Lys Leu Ala Ala Ile Trp Gln Asp Val Leu Val Arg Glu Lys 4675 4680 4685 Ala Val Gly Val Thr Asp Asn Phe Phe Asp Leu Gly Gly His Ser Leu 4690 4695 4700 Arg Ala Thr Thr Leu Val Ser Lys Met His Lys Glu Leu Gly Val Glu 4705 4710 4715 4720 Phe Pro Leu Arg Asp Val Phe Arg Tyr Ser Thr Val Glu Glu Met Ala 4725 4730 4735 Ala Ala Met Glu Arg Leu Glu Ile Gly Ser Phe Thr Ala Ile Pro Ala 4740 4745 4750 Ala Glu Pro Ser Glu Tyr Tyr Pro Leu Ser Ser Ala Gln Lys Arg Leu 4755 4760 4765 Tyr Ile Leu Asn Gln Leu Glu Gly Ala Glu Leu Ser Tyr Asn Ile Pro 4770 4775 4780 Gly Ala Met Leu Leu Glu Gly Glu Leu Asp Arg Gln Arg Phe Glu Glu 4785 4790 4795 4800 Ala Phe Arg Gly Leu Val Ala Arg His Glu Thr Leu Arg Thr Gly Phe 4805 4810 4815 Glu Met Val Lys Gly Glu Ala Val Gln Arg Ile Tyr Glu Glu Ala Ala 4820 4825 4830 Phe Gln Val Glu Tyr Val Gln Ile Ser Ala Glu Gln Ala Glu Glu Thr 4835 4840 4845 Val Arg Gln Phe Val Arg Pro Phe Asp Leu Ala Lys Pro Pro Leu Leu 4850 4855 4860 Arg Val Gly Leu Ala Glu Leu Ala Pro Asp Arg His Ile Leu Met Phe 4865 4870 4875 4880 Asp Thr His His Ile Val Ser Asp Gly Val Ser Ile Asp Val Leu Ile 4885 4890 4895 Glu Glu Phe Val Arg Leu Tyr Ser Gly Glu Gln Leu Glu Pro Leu Arg 4900 4905 4910 Ile Gln Tyr Lys Asp Tyr Ala Val Trp Gln Gln Ser Asp Glu Gln Lys 4915 4920 4925 Ala Gln Leu Ala Lys Gln Glu Ala Tyr Trp Leu Asp Met Phe Arg Gly 4930 4935 4940 Glu Leu Pro Val Leu Glu Leu Pro Met Asp Tyr Pro Arg Pro Ala Met 4945 4950 4955 4960 Gln Ser Tyr Glu Gly Arg Thr Leu Gln Leu Phe Met His Ser Glu Lys 4965 4970 4975 Ser Glu Gly Leu Lys Arg Leu Ala Ala Glu Asn Gly Ala Thr Leu Tyr 4980 4985 4990 Met Val Leu Leu Ala Gly Tyr Thr Ile Leu Leu His Lys Tyr Thr Gly 4995 5000 5005 Gln Glu Asp Val Val Val Gly Thr Pro Ile Ala Gly Arg Asn His Ser 5010 5015 5020 Asp Val Gln Pro Leu Ile Gly Met Phe Val Asn Thr Leu Ala Ile Arg 5025 5030 5035 5040 Ser Tyr Pro Ala Ala Gly Lys Thr Phe Leu Asp Tyr Leu Lys Glu Ile 5045 5050 5055 Lys Glu Thr Thr Leu Gly Ala Phe Glu His Gln Asn Tyr Pro Phe Glu 5060 5065 5070 Glu Leu Val Asp Lys Val Asn Val Ala Arg Asp Leu Ser Arg Asn Pro 5075 5080 5085 Leu Phe Asp Thr Met Phe Ala Leu Gln Asn Thr Glu Asn Leu Glu Ile 5090 5095 5100 Gln Leu Pro Gly Leu His Leu Ser Thr Tyr Ala Ser Glu Glu Ile Val 5105 5110 5115 5120 Ser Lys Phe Asp Leu Ser Leu Asp Val Thr Glu Ile Glu Glu Gly Leu 5125 5130 5135 Glu Tyr Leu Phe Glu Tyr Ala Thr Ala Leu Tyr Lys Thr Glu Thr Val 5140 5145 5150 Glu Lys Leu Ala Ala His Tyr Leu Gln Leu Leu Glu Ser Ile Leu Arg 5155 5160 5165 Asn Pro Ser Ala Thr Ile Ala Glu Leu Gly Ile Leu Thr Pro Ala Glu 5170 5175 5180 Lys Glu Gln Ile Leu Gly Ala Phe Asn Pro Ala Gln Pro Gly Ala Ala 5185 5190 5195 5200 Pro Ala Ala Ala Phe His Arg Leu Phe Glu Glu Gln Ala Glu Arg Thr 5205 5210 5215 Pro Glu Glu Ala Ala Val Val Tyr Glu Asn Asp Arg Leu Thr Tyr Ala 5220 5225 5230 Glu Leu Asn Glu Arg Ala Asn Arg Leu Ala Ala Thr Leu Arg Ala Ser 5235 5240 5245 Gly Ile Gly Arg Glu Thr Ile Val Gly Ile Leu Ala Glu Arg Ser Val 5250 5255 5260 Asp Leu Leu Val Ala Val Leu Ala Val Trp Lys Ala Gly Gly Ala Tyr 5265 5270 5275 5280 Val Pro Leu Asp Pro Asp Tyr Pro Ala Asp Arg Val Arg Phe Met Leu 5285 5290 5295 Glu Asp Ser Gly Ala Lys Val Leu Leu Thr Gln Thr Pro Leu Arg Glu 5300 5305 5310 Arg Ala Glu Ala Trp Leu Asp Glu Glu Glu Leu Ala Leu Ala Ala Val 5315 5320 5325 Leu Tyr Leu Asp Asp Glu Ala Ser Tyr Ser Glu Glu Arg Ala Asn Ala 5330 5335 5340 Ser Val Val Ser Gly Ile Val Ser Gly Ile Val Ser Gly Lys Leu Thr 5345 5350 5355 5360 Asp Ala Val Asp Asp Gly Asp Glu Ser His Gln Asn Val Gly Thr Asp 5365 5370 5375 Ser Phe His Glu Ala Arg Pro Glu Asp Leu Ala Tyr Val Ile Tyr Thr 5380 5385 5390 Ser Gly Thr Thr Gly Lys Pro Lys Gly Val Met Ile Glu His Arg Ser 5395 5400 5405 Leu Val Asn Thr Ala Ala Gly Tyr Arg Arg Glu Tyr Arg Leu Asp Gln 5410 5415 5420 Phe Pro Val Arg Leu Leu Gln Leu Ala Ser Phe Ser Phe Asp Val Phe 5425 5430 5435 5440 Val Gly Asp Ile Ala Arg Thr Leu Tyr Asn Gly Gly Thr Met Val Ile 5445 5450 5455 Val Pro Lys Asp Asp Arg Ile Asp Pro Ser Arg Leu His His Trp Met 5460 5465 5470 Glu Arg Glu Arg Val Thr Ile Phe Glu Ser Thr Pro Ala Leu Ile Val 5475 5480 5485 Pro Phe Leu Glu Tyr Val His Glu Gln Gln Leu Asp Met Ser Trp Met 5490 5495 5500 Glu Leu Leu Ile Thr Ser Ser Asp Ser Cys Ser Val Ala Asp Tyr Arg 5505 5510 5515 5520 Thr Leu Gln Glu Arg Phe Gly Ser Leu Phe Arg Ile Ile Asn Ala Tyr 5525 5530 5535 Gly Val Thr Glu Ala Ala Ile Asp Ser Ser Phe Tyr Asp Glu Glu Leu 5540 5545 5550 Ala Lys Leu Pro Gln Thr Gly Asn Val Pro Ile Gly Lys Ala Trp Leu 5555 5560 5565 Asn Ala Lys Phe Tyr Ile Val Asp Ala His Leu Asn Pro Val Pro Val 5570 5575 5580 Gly Val Leu Gly Glu Leu Ala Ile Gly Gly Val Gly Val Ala Arg Gly 5585 5590 5595 5600 Tyr Leu Asn Arg Pro Glu Leu Thr Glu Glu Lys Phe Val Asp Ser Pro 5605 5610 5615 Phe Ala Ala Gly Glu Arg Leu Tyr Arg Thr Gly Asp Leu Ala Arg Trp 5620 5625 5630 Met Glu Asp Gly Asn Val Asp Phe Ile Gly Arg Ile Asp Asn Gln Ala 5635 5640 5645 Lys Ile Arg Gly Tyr Arg Ile Glu Thr Gly Glu Val Glu Ala Lys Leu 5650 5655 5660 Leu Ser Val Gly Gly Val Lys Glu Ala Val Val Val Val Arg Glu Asp 5665 5670 5675 5680 Gln Glu Gly Gln Lys Ala Leu Cys Ala Tyr Tyr Thr Ala Glu Glu Gly 5685 5690 5695 Leu Thr Ala Ala Asp Leu Lys Arg Ala Ile Ala Ser Glu Leu Pro Gly 5700 5705 5710 Tyr Met Ile Pro Ser Tyr Phe Val Glu Leu Glu Arg Leu Pro Leu Thr 5715 5720 5725 Pro Asn Gly Lys Ile Asp Arg Lys Ala Leu Pro Ala Pro Glu Gly Gly 5730 5735 5740 Ala Ser Ala Gly Arg Glu Tyr Val Ala Pro Arg Thr Glu Leu Glu Ala 5745 5750 5755 5760 Lys Leu Val Ala Ile Trp Gln Asp Val Leu Gly Pro Val Thr Ile Gly 5765 5770 5775 Val Thr Asp Asn Phe Phe Asp Leu Gly Gly His Ser Leu Arg Ala Thr 5780 5785 5790 Thr Leu Val Ser Lys Val His Lys Glu Leu Ser Val Asp Leu Pro Leu 5795 5800 5805 Arg Asp Val Phe Arg His Ser Thr Ile Glu Ala Met Ala Glu Ala Ile 5810 5815 5820 Ser Gln Leu Glu Arg Gln Glu His Leu Ser Ile Pro Val Leu Asp Lys 5825 5830 5835 5840 Arg Asp Tyr Tyr Pro Leu Ser Ser Val Gln Lys Arg Leu Tyr Ile Gln 5845 5850 5855 Gln Gln Met Glu Gly Ala Glu Leu Ser Tyr Asn Met Ser Gly Met Thr 5860 5865 5870 Val Leu Val Gly Arg Leu Glu Arg Asn Gln Phe Glu Ala Ala Leu Lys 5875 5880 5885 Gly Leu Ile Ala Arg His Glu Ile Leu Arg Thr Gly Phe Glu Met Val 5890 5895 5900 Asp Gly Glu Pro Val Gln Arg Ile Tyr Pro Asp Leu Lys Phe Ala Val 5905 5910 5915 5920 Glu Tyr Thr Lys Ala Thr Glu Ser Glu Thr Lys Ser Ile Val Asp Gly 5925 5930 5935 Phe Val Arg Val Phe Asp Leu Glu Arg Pro Pro Leu Leu Arg Val Gly 5940 5945 5950 Leu Val Glu Trp Glu Ala Glu Arg His Leu Leu Met Leu Asp Ile His 5955 5960 5965 His Ile Val Thr Asp Gly Met Ser Met Gly Ile Phe Val Glu Glu Leu 5970 5975 5980 Leu Arg Leu Tyr Asn Gly Glu Thr Met Glu Pro Leu Arg Ile Gln Tyr 5985 5990 5995 6000 Lys Glu Phe Ala Ala Trp Gln Gln Ser Glu Pro Val Lys Glu Arg Leu 6005 6010 6015 Lys Arg Gln Glu Ala Tyr Trp Leu Asp Val Leu Glu Gly Glu Leu Pro 6020 6025 6030 Thr Leu Glu Leu Pro Thr Asp Phe Val Arg Pro Ala Ala Arg Ser Phe 6035 6040 6045 Glu Gly Asp Val Leu Pro Phe Ser Ile Asp Lys Gln Met Thr Asp Ser 6050 6055 6060 Leu Gln Arg Ile Ala Asp Glu Asn Gly Ala Thr Leu Tyr Met Val Leu 6065 6070 6075 6080 Leu Ala Ala Tyr Ser Ile Leu Leu Ser Lys Tyr Ser Gly Gln Glu Asp 6085 6090 6095 Phe Ile Val Gly Thr Pro Val Ser Gly Arg Thr His Ala Asp Leu Glu 6100 6105 6110 Pro Leu Ile Gly Met Phe Val Asn Thr Leu Ala Ile Arg His Tyr Pro 6115 6120 6125 Ser Gly Glu Lys Thr Phe Leu Ala Tyr Leu Asn Glu Val Lys Glu Thr 6130 6135 6140 Met Leu Gly Ala Tyr Asp His Gln Asp Tyr Pro Phe Glu Glu Leu Val 6145 6150 6155 6160 Lys Lys Leu Gln Ala Pro Arg Asp Gln Gly Arg Asn Pro Val Phe Asp 6165 6170 6175 Val Met Phe Ala Leu Glu Thr Lys Glu Asp Asn Val Gln Ser Phe Gly 6180 6185 6190 Asp Ile Lys Ile Glu Ser Tyr Pro Glu Thr His Thr Val Ser Gln Phe 6195 6200 6205 Asp Leu Thr Leu Val Ile Ser Leu Leu Asp Glu Gly Met Asn Gly Gln 6210 6215 6220 Phe Glu Tyr Ala Thr Lys Leu Phe Thr Arg Asn Leu Ile Asp Asn Phe 6225 6230 6235 6240 Ala Gln Asp Leu Leu Val Ile Ile Thr Gln Ile Cys Glu Gln Pro Ser 6245 6250 6255 Val Leu Leu Lys Asp Ile Ser Leu Asn Gly Gln Ser Glu Gln Glu Gln 6260 6265 6270 Asp Val Leu Glu Thr Ile Asp Ile Ile Phe 6275 6280 <210> 4 <211> 4966 <212> PRT <213> Paenibacillus polymyxa ATCC21830 <400> 4 Met Ala Phe Glu Lys Glu Thr Leu Phe Trp Asn Glu Lys Phe Gly Ser 1 5 10 15 Asp Asp Tyr Thr Leu Thr Arg Leu Pro Tyr Ser Lys Ala Pro Ser Ser 20 25 30 Leu Thr Pro Ile Met Thr Thr Val Gly Gly Thr Leu Ser Glu Glu Val 35 40 45 Ala Gln Arg Val Leu Gln Met Ser Lys Gly Ala Pro Leu Ala Thr Phe 50 55 60 Met Ile Leu Leu Ala Gly Val Gln Ser Leu Leu Tyr Lys Tyr Thr Gly 65 70 75 80 Ala Ser Asp Ile Leu Val Gly Met Pro Val Val Arg Lys Pro Thr Glu 85 90 95 Thr Arg Arg Ser Val Asn His Thr Ile Ile Leu Lys Asn Ser Leu Ser 100 105 110 Ala Gly Ala Thr Phe Lys Thr Leu Leu Ser Glu Leu Arg Thr Ser Leu 115 120 125 Pro Glu Ala Ile Gln His Gln His Ile Pro Phe Leu Lys Met Thr Glu 130 135 140 Lys Leu Asp Leu Gln Tyr Ala Asp Gly Ile Pro Val Val His Thr Leu 145 150 155 160 Val Ser Leu Lys Glu Leu His Leu Asp Glu Ile Gly Gln Asn Val Val 165 170 175 Thr Asp Cys Ser Phe Glu Phe Ser Leu Thr Gly Gly Thr Ile Gln Leu 180 185 190 Ala Leu Ser Tyr Asn Glu His Leu Tyr Asp Ser Glu Phe Met Thr Arg 195 200 205 Val Val Gly His Leu Asn Arg Leu Leu Ala Val Gly Leu His Glu Leu 210 215 220 Glu Leu Glu Ile Val Arg Ala Asp Met Leu Ser Glu Asp Glu Lys Phe 225 230 235 240 Gln Leu Leu Gln Ser Phe Asn Asp Thr Glu Lys Asp Tyr Pro Arg Asp 245 250 255 Arg Thr Ile His Gln Leu Val Glu Glu Gln Ala Lys Arg Val Pro Glu 260 265 270 Ala Thr Ala Val Val Phe Glu Gly Arg Arg Leu Ser Tyr Ala Glu Leu 275 280 285 Asn Glu Arg Ala Asn Arg Leu Ala Arg Thr Leu Arg Ser Val Gly Val 290 295 300 Leu Pro Asn Gln Leu Val Gly Leu Met Ala Arg Arg Ser Leu Glu Thr 305 310 315 320 Val Val Gly Ile Leu Ala Val Leu Lys Ala Gly Gly Ala Tyr Val Pro 325 330 335 Ile Asp Pro Glu Tyr Pro Glu Glu Arg Ile Arg Tyr Ile Leu Glu Asn 340 345 350 Ser Asn Ala Gln Leu Leu Leu Thr Gln Arg Glu Leu Leu Gln Gln Val 355 360 365 Pro Phe Glu Gly Thr Val Leu Ala Leu Asp Asp Glu Gln Thr Tyr Ser 370 375 380 Asp Asp Gly Ser Asn Leu Glu Pro Ala Ser Gly Pro Asn Asp Leu Ala 385 390 395 400 Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly Lys Pro Lys Gly Val Met 405 410 415 Leu Glu His Arg Gly Leu Val Ser Leu Lys Leu Met Phe Ala Asp Arg 420 425 430 Leu Gly Ile Thr Glu His Asp Arg Ile Val Gln Phe Ala Ser Leu Ser 435 440 445 Phe Asp Ala Ser Cys Trp Glu Met Phe Lys Ala Leu Tyr Phe Gly Ala 450 455 460 Ala Leu Tyr Ile Pro Thr Ala Glu Thr Ile Leu Asp Thr Arg Leu Phe 465 470 475 480 Glu Ser Tyr Met Asn Glu His Ala Ile Thr Ala Ala Ile Leu Pro Pro 485 490 495 Thr Tyr Ser Ala Tyr Leu Asn Pro Asp Arg Leu Pro Ser Leu Thr Lys 500 505 510 Leu Val Thr Gly Gly Ser Ala Val Ser Ala Glu Phe Val Gln Gln Trp 515 520 525 Lys Pro Lys Val His Tyr Phe Asn Ala Tyr Gly Pro Thr Glu Ala Ser 530 535 540 Ile Val Thr Thr Leu Trp Asp Ala Asp Glu Glu Gln Ser Glu Arg Arg 545 550 555 560 Val Ile Pro Ile Gly Arg Pro Leu Ala Asn His Arg Ile Phe Ile Leu 565 570 575 Asp Ala His Leu Gln Leu Val Pro Pro Gly Val Asp Gly Glu Leu Cys 580 585 590 Val Ala Gly Val Gly Leu Ala Arg Gly Tyr Leu Asn His Pro Glu Leu 595 600 605 Thr Ala Glu Lys Phe Val Glu His Pro Phe Ala Pro Gly Glu Arg Leu 610 615 620 Tyr Arg Thr Gly Asp Leu Ala Arg Trp Leu Pro Asp Gly Asn Ile Glu 625 630 635 640 Tyr Leu Gly Arg Ile Asp His Gln Val Lys Ile Arg Gly Phe Arg Ile 645 650 655 Glu Ile Gly Glu Ile Glu Glu Gln Leu Leu Lys Ile Asp Ser Val Gln 660 665 670 Glu Thr Ile Val Ile Ala Arg Glu Gly Lys Ser Gly Gln Glu Leu Cys 675 680 685 Ala Tyr Leu Val Ala Gly His Pro Leu Thr Leu Gly Glu Leu Arg Ser 690 695 700 Ala Leu Ala Gln Lys Leu Pro Asn Tyr Met Ile Pro Ala His Phe Val 705 710 715 720 Gln Leu Pro Arg Met Pro Leu Thr Pro Asn Asp Lys Ile Asp Arg Lys 725 730 735 Ala Leu Pro Val Pro Glu Gly Asn Ala Leu Thr Gly Gly Ala Tyr Val 740 745 750 Ala Pro Arg Asn Glu Ala Glu Arg Thr Leu Ala Asp Val Trp Gln Ala 755 760 765 Val Leu Asn Ala Asp Arg Val Gly Val Thr Asp His Phe Phe Glu Leu 770 775 780 Gly Gly Asp Ser Ile Lys Ser Ile Gln Val Ser Ser Arg Leu His Gln 785 790 795 800 Ala Gly Tyr Lys Leu Glu Ile Arg Asp Leu Phe Lys Tyr Pro Thr Ile 805 810 815 Ser Gln Leu Ser Leu His Val Lys Pro Ile Gly Arg Thr Ile Asp Gln 820 825 830 Gly Glu Ile Thr Gly Glu Thr Ala Leu Thr Pro Ile Gln His Trp Phe 835 840 845 Phe Glu Ser Ser Phe Ala Asp Pro His His Phe Asn Gln Ser Val Ile 850 855 860 Leu Tyr Arg Lys Glu Arg Phe Asp Glu Glu Thr Val Arg Gln Val Leu 865 870 875 880 Gln Lys Leu Ala Glu His His Asp Ala Leu Arg Met Val Phe Arg Lys 885 890 895 Thr Glu Gln Gly Phe Ser Ala Arg Asn Arg Ala Ile Gln Glu Gly Gly 900 905 910 Leu Phe Thr Leu Asp Val Phe Asp Phe Lys Asp Ala Lys Asn Thr Ala 915 920 925 Gln Ala Val Glu Ala Lys Ala Thr Asp Ile Gln Ala Gly Ile Asp Leu 930 935 940 Glu Asn Gly Pro Leu Val Lys Ala Gly Leu Phe Arg Cys Ala Asp Gly 945 950 955 960 Asp His Leu Leu Leu Ala Val His His Ala Val Val Asp Gly Val Ser 965 970 975 Trp Arg Ile Leu Met Glu Asp Phe Ala Leu Gly Tyr Glu Gln Ala Gly 980 985 990 Lys Ser Glu Glu Ile Arg Phe Pro Ala Lys Thr Asp Ala Tyr Arg Thr 995 1000 1005 Trp Ser Glu Gln Leu Ala Ala Tyr Val Gln Ser Pro Glu Met Ala Lys 1010 1015 1020 Glu Arg Ala Tyr Trp Gln Ala Val Glu Gln Ile Ala Val Pro Ala Val 1025 1030 1035 1040 Pro Lys Asp Leu Glu Ala Asp Val Thr Thr Gln Gln Asp Ser Glu Ser 1045 1050 1055 Leu Phe Val Cys Leu Thr Pro Glu Glu Thr Glu Leu Leu Leu Lys Arg 1060 1065 1070 Val His Arg Ala Tyr Asn Thr Glu Met Asn Asp Ile Leu Val Thr Ala 1075 1080 1085 Leu Gly Ile Ala Ile Arg Lys Trp Thr Gly His Glu Arg Val Arg Ile 1090 1095 1100 Asn Leu Glu Gly His Gly Arg Glu Ser Ile Gly Thr Asp Ile Asp Ile 1105 1110 1115 1120 Thr Arg Thr Val Gly Trp Phe Thr Thr Lys Phe Pro Val Val Leu Glu 1125 1130 1135 Pro Glu Thr Asp Arg Asp Leu Ala Tyr Gln Ile Lys Gln Val Lys Glu 1140 1145 1150 Ser Leu Arg Arg Ile Pro Asn Lys Gly Leu Gly Tyr Gly Val Cys Arg 1155 1160 1165 Tyr Leu Ser Lys Ser Glu Asp Gly Phe Val Trp Gly Ala Glu Pro Glu 1170 1175 1180 Ile Asn Phe Asn Tyr Leu Gly Gln Phe Asp Asp Asp Val Asn Gln Asp 1185 1190 1195 1200 Glu Ile Gly Ile Ser Ser Tyr Ser Ser Gly Ser Pro Ala Ser Asp Arg 1205 1210 1215 Gln Ala Arg Ser Phe Val Leu Asp Ile Asn Gly Met Val Leu Asp Gly 1220 1225 1230 Ala Leu Ser Leu Asp Leu Ser Tyr Ser Arg Lys Gln Tyr Arg Lys Glu 1235 1240 1245 Thr Ile Glu Ala Phe Ala Gln Arg Leu Glu Gln Ser Leu Arg Glu Leu 1250 1255 1260 Ile Thr His Cys Ala Gly Lys Glu Asn Thr Glu Leu Thr Pro Ser Asp 1265 1270 1275 1280 Val Gln Phe Lys Gly Leu Thr Ile Ala Glu Leu Glu Gln Ile Ala Gln 1285 1290 1295 Arg Ser Gly His Leu Gly Glu Ile Glu Asn Ile Tyr Ser Leu Thr Pro 1300 1305 1310 Met Gln Lys Gly Met Trp Phe His Ser Ala Leu Asp Arg Gln Thr Ala 1315 1320 1325 Ala Tyr Phe Glu Gln Thr Arg Phe Thr Met Arg Gly Ala Leu Asp Val 1330 1335 1340 Gln Leu Phe Glu Arg Ser Trp Met Glu Leu Ala Lys Arg His Leu Val 1345 1350 1355 1360 Leu Arg Ala Asn Phe Val Lys Gly Pro Ala Gly Glu Pro Leu Gln Ile 1365 1370 1375 Ile Tyr Arg Asp Lys Pro Val Gly Phe Glu Tyr Glu Glu Leu Leu His 1380 1385 1390 Leu Gln Ala Asp Glu Lys Gln Thr Tyr Leu Asp Lys Lys Ala Glu Asp 1395 1400 1405 Asp Lys Leu Arg Gly Phe Asp Leu Glu His Asp Ala Leu Val Arg Val 1410 1415 1420 Thr Ile Leu Arg Thr Glu Glu Gln Ser Tyr His Val Leu Trp Ser Phe 1425 1430 1435 1440 Gln His Ile Leu Met Asp Gly Trp Cys Leu Pro Gln Leu Thr Gln Glu 1445 1450 1455 Leu Phe Glu Thr Tyr Ser Ala Leu Ala Ser Gly Lys Gln Pro Ala Gly 1460 1465 1470 Asp Lys Gly Ser Asp Tyr Gly Ala Tyr Ile Glu Trp Leu Glu Lys Gln 1475 1480 1485 Asp Asp Gln Ala Ala Ser Gly Tyr Trp Thr Ala Phe Leu Ala Gly Tyr 1490 1495 1500 Glu Gly Gln Thr Val Leu Pro Gly Gln Lys Glu Pro Ala Pro Asn Gly 1505 1510 1515 1520 Arg Phe Thr Ala Asp His Val Thr Ala Glu Leu Gly Lys Asp Leu Ser 1525 1530 1535 Glu Arg Met Asp Arg Val Ala Lys Gln Arg Leu Val Thr Val Asn Thr 1540 1545 1550 Leu Leu Gln Ala Ala Trp Gly Val Met Leu Gln Lys Tyr Asn Gly Thr 1555 1560 1565 Asn Asp Ala Val Phe Gly Ser Val Val Ala Gly Arg Pro Ala Glu Ile 1570 1575 1580 Pro Gly Ile Glu Ser Met Ile Gly Leu Phe Ile Asn Thr Val Pro Val 1585 1590 1595 1600 Arg Val Thr Ser Glu Ala Asp Thr Val Phe Ala Asp Leu Met Ala Lys 1605 1610 1615 Leu Gln Glu Arg Ala Leu Glu Ser Gly Arg Tyr Asp Tyr Tyr Pro Leu 1620 1625 1630 Tyr Glu Ile Gln Ala His Ser Val Gln Lys Gln Asn Leu Ile Asn His 1635 1640 1645 Ile Ile Ala Phe Glu Asn Tyr Pro Val Asp Glu Gln Met Glu Gln Ala 1650 1655 1660 Gly Asp Gln Gln His Gly Asp Leu Thr Ile Ala Asp Val Gln Met Glu 1665 1670 1675 1680 Glu Gln Thr Asn Tyr Asn Phe Asn Val Thr Val Val Pro Gly Asp Glu 1685 1690 1695 Ile Glu Ile Arg Phe Asp Phe Asn Ala Glu Val Phe Asp Lys Asp Ser 1700 1705 1710 Ile Glu Arg Leu Lys Gly His Leu Val His Leu Leu Glu Gln Val Thr 1715 1720 1725 Asp Asn Pro Glu Ile Thr Val Gly Glu Leu Glu Leu Val Thr Glu Ala 1730 1735 1740 Glu Lys Ala Asp Leu Leu Gly Arg Phe Asn Asp Thr Thr Thr Glu Phe 1745 1750 1755 1760 Pro Arg Gly Lys Thr Leu Ile Gln Leu Phe Glu Glu Gln Ala Glu Arg 1765 1770 1775 Ile Pro Asp Ala Ala Ala Ile Ser Leu Asn Glu Gln Glu Leu Thr Tyr 1780 1785 1790 Arg Glu Leu Asn Glu Arg Val Asn Arg Leu Ala Arg Thr Leu Arg Ser 1795 1800 1805 His Gly Ile Ser Lys Gly Arg Leu Val Ala Ile Met Ala Glu Arg Ser 1810 1815 1820 Ile Glu Met Val Val Gly Met Leu Ala Ala His Lys Ala Gly Ala Ala 1825 1830 1835 1840 Tyr Val Pro Ile Asp Pro Glu Tyr Pro Glu Glu Arg Ile Arg Phe Leu 1845 1850 1855 Ile Glu Asp Ser Gly Ala Gln Val Met Leu Thr Gln Ser Arg Leu Arg 1860 1865 1870 Glu Arg Leu Ala Gly Ser Asp Ser Val Ile Leu Leu Asp Asp Glu Ser 1875 1880 1885 Phe Tyr His Glu Asp Gly Thr Asn Leu Asn Pro Gly Thr Glu Ala Thr 1890 1895 1900 Asp Leu Ala Cys Val Ile Tyr Thr Ser Gly Thr Thr Gly Lys Pro Lys 1905 1910 1915 1920 Gly Asn Leu Val Ser His Arg Asn Ile Val Arg Ile Val Arg Asn Thr 1925 1930 1935 Asn Tyr Ile Asp Ile Thr Glu Arg Asp His Val Leu Gln Leu Ser Ser 1940 1945 1950 Tyr Ser Phe Asp Gly Ala Ile Phe Asp Val Phe Gly Ala Leu Thr Asn 1955 1960 1965 Gly Ala Arg Leu Val Leu Val Pro Arg Glu Thr Leu Leu Glu Ile Gly 1970 1975 1980 Arg Leu Ala Asp Leu Ile Gln His Glu Arg Ile Ser Val Met Leu Ile 1985 1990 1995 2000 Thr Thr Ala Tyr Phe Asn Val Leu Val Asp Val Asn Val Asp Cys Leu 2005 2010 2015 Arg Asp Val Arg Ala Ile Leu Phe Gly Gly Glu Arg Val Ser Val Gly 2020 2025 2030 His Val Arg Arg Ala Leu Ala His Ile Gly Pro Gly Arg Leu Asn His 2035 2040 2045 Leu Tyr Gly Pro Ser Glu Ser Thr Val Tyr Thr Thr Tyr Leu Pro Val 2050 2055 2060 Asp Phe Val Asp Glu Ser Ala Val Thr Val Pro Ile Gly Arg Pro Ile 2065 2070 2075 2080 Ser Asn Thr Thr Val Tyr Ile Val Asp Ser Arg Asn Lys Leu Leu Pro 2085 2090 2095 Ile Gly Val Ala Gly Glu Leu Cys Val Gly Gly Glu Gly Leu Val Arg 2100 2105 2110 Gly Tyr Asn Asn Arg Pro Glu Leu Thr Ala Glu Lys Phe Val Asp Asn 2115 2120 2125 Pro Phe Val Pro Gly Glu Arg Met Tyr Arg Thr Gly Asp Leu Ala Lys 2130 2135 2140 Trp Leu Pro Asp Gly Thr Ile Glu Tyr Val Gly Arg Thr Asp Asp Gln 2145 2150 2155 2160 Val Lys Ile Arg Gly Phe Arg Ile Glu Leu Gly Glu Ile Glu Ala Gln 2165 2170 2175 Leu Gln Lys Val Glu Gly Ile Arg Lys Thr Thr Val Phe Ala Arg Glu 2180 2185 2190 Asn Ala Ser Gly Glu Lys Gln Leu Cys Ala Tyr Tyr Glu Ala Asp Arg 2195 2200 2205 Glu Leu Ser Ala Ala Glu Leu Lys Asn Val Ile Ser Gln Glu Leu Pro 2210 2215 2220 Ala Tyr Met Ile Pro Ala Tyr Leu Ile Gln Leu Glu Arg Leu Pro Leu 2225 2230 2235 2240 Thr Ala Asn Gly Lys Val Asp Arg Arg Ser Leu Pro Thr Pro Glu Glu 2245 2250 2255 Ser Leu Gln Pro Gly Glu Gly Arg Thr Pro Pro Arg Thr Pro Leu Glu 2260 2265 2270 Ala Ser Leu Ala Gly Ile Trp Lys Ser Val Leu Ala Leu Glu His Ile 2275 2280 2285 Gly Val His Asp Asn Phe Phe Asp Leu Gly Gly His Ser Leu Arg Ala 2290 2295 2300 Thr Thr Leu Val Ser Lys Val His Gln Glu Leu Asn Val Glu Leu Pro 2305 2310 2315 2320 Leu Arg Asp Val Phe Arg Tyr Ser Thr Ile Glu Glu Met Ala Leu Ala 2325 2330 2335 Ile Thr Arg Ile Gly Glu Gln Ser Phe Ser Ser Ile Pro Leu Ala Gly 2340 2345 2350 Val Arg Glu Tyr Tyr Pro Leu Ser Ser Ala Gln Lys Arg Leu Phe Ile 2355 2360 2365 Leu Asn Gln Leu Glu Gly Ser Asp Gln Ser Tyr Asn Met Pro Gly Val 2370 2375 2380 Leu Leu Leu Glu Gly Ser Ile Asp Arg Ser Leu Leu Glu Lys Ala Phe 2385 2390 2395 2400 Arg Gly Leu Ile Val Arg His Glu Thr Leu Arg Thr Gly Phe Glu Ile 2405 2410 2415 Val Gln Gly Glu Ala Val Gln Arg Ile Tyr Glu Ser Val Asp Phe Ala 2420 2425 2430 Val Glu Tyr Arg His Ala Ser Glu Glu Glu Ala Pro Glu Val Val Gln 2435 2440 2445 Ala Phe Ile Arg Pro Phe Asp Leu Ala Lys Pro Pro Leu Leu Arg Val 2450 2455 2460 Glu Leu Val Glu Leu Ala Thr Glu Arg Tyr Leu Leu Met Phe Asp Met 2465 2470 2475 2480 His His Ile Val Ser Asp Gly Val Ser Met Asp Val Leu Val Glu Glu 2485 2490 2495 Leu Val Arg Leu Tyr Gly Gly Glu Ser Leu Glu Pro Leu Arg Ile Gln 2500 2505 2510 Tyr Lys Asp Tyr Ala Val Trp Gln Gln Ser Asp Glu Gln Lys Ala Gln 2515 2520 2525 Leu Lys Arg Glu Glu Ala Tyr Trp Leu Asp Arg Tyr Arg Gly Glu Leu 2530 2535 2540 Pro Val Leu Glu Met Pro Thr Asp Tyr Pro Arg Pro Ala Val Gln Ser 2545 2550 2555 2560 Phe Glu Gly Gln Thr Leu Thr Ser Phe Val Asp Glu Ala Thr Asn Glu 2565 2570 2575 Gly Leu Lys Gln Leu Ala Ala Gln Arg Gly Thr Thr Leu Tyr Met Val 2580 2585 2590 Leu Leu Ala Ala Tyr Thr Val Leu Leu His Lys Tyr Thr Gly Gln Asp 2595 2600 2605 Asp Leu Ile Val Gly Thr Ser Ile Ala Gly Arg Thr His Gly Asp Thr 2610 2615 2620 Gln Pro Leu Ile Gly Met Phe Val Asn Thr Leu Ala Leu Arg Asn Tyr 2625 2630 2635 2640 Pro Ala Ser Glu Lys Thr Phe Leu Ser Tyr Leu Glu Glu Val Lys Glu 2645 2650 2655 Thr Thr Leu Gly Ala Tyr Glu His Gln Asn Tyr Pro Phe Glu Glu Leu 2660 2665 2670 Val Asp Lys Val Gln Val Ser Arg Asp Leu Ser Arg Asn Pro Leu Phe 2675 2680 2685 Asp Thr Met Phe Ser Leu Gln Asn Leu Glu Asp Lys Glu Phe Glu Leu 2690 2695 2700 Glu Gly Leu Lys Leu Ser Pro Tyr Pro Ser Glu Tyr Gly Thr Ala Lys 2705 2710 2715 2720 Phe Asp Leu Ser Val Asp Val Thr Glu Glu Asn Gly Gly Leu Glu Cys 2725 2730 2735 Ser Phe Glu Phe Ala Thr Ala Leu Tyr Lys Glu Ser Thr Ile Arg Arg 2740 2745 2750 Leu Ser Thr His Phe Gly His Leu Leu Ala Glu Ile Val Ser Cys Pro 2755 2760 2765 Asp Ala Lys Ile Ala Glu Leu Asn Leu Leu Thr Ala Glu Glu Glu Lys Glu 2770 2775 2780 Gln Ile Leu Gly Ala Phe Asn Pro Ala Gln Pro Glu Val Ala Pro Ala 2785 2790 2795 2800 Ala Ala Phe His Arg Leu Phe Glu Glu Gln Ala Glu Arg Thr Pro Glu 2805 2810 2815 Ala Ala Ala Val Val Tyr Glu Asn Asp Arg Leu Thr Tyr Ala Glu Leu 2820 2825 2830 Asn Glu Arg Ala Asn Arg Leu Ala Ala Thr Leu Arg Ala Ser Gly Ile 2835 2840 2845 Gly Arg Glu Thr Ile Val Gly Ile Leu Ala Glu Arg Ser Val Asp Leu 2850 2855 2860 Leu Val Ala Val Leu Ala Val Trp Lys Ala Gly Gly Ala Tyr Val Pro 2865 2870 2875 2880 Leu Asp Pro Asp Tyr Pro Ala Glu Arg Val Arg Phe Met Leu Glu Asp 2885 2890 2895 Ser Gly Ala Lys Val Leu Leu Thr Gln Thr Ala Leu Arg Glu Arg Ala 2900 2905 2910 Glu Ala Trp Leu Gly Glu Glu Glu Leu Ala Leu Ala Ala Val Leu Tyr 2915 2920 2925 Leu Asp Asp Glu Ala Ser Tyr Ser Glu Glu Arg Ala Asn Ala Pro Ile 2930 2935 2940 Val Phe Gly Ile Ser Ser Lys Leu Thr Asp Ala Val Asp Asp Gly Asp 2945 2950 2955 2960 Glu Ser Pro Gln Asn Val Gly Thr Asp Ser Phe His Glu Ala Arg Pro 2965 2970 2975 Glu Asp Leu Ala Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly Lys Pro 2980 2985 2990 Lys Gly Val Met Ile Glu His Arg Ser Leu Val Asn Thr Ala Ala Gly 2995 3000 3005 Tyr Arg Gln Glu Tyr Arg Leu Asp Gln Phe Pro Val Arg Leu Leu Gln 3010 3015 3020 Leu Ala Ser Phe Ser Phe Asp Val Phe Val Gly Asp Ile Ala Arg Thr 3025 3030 3035 3040 Leu Tyr Asn Gly Gly Thr Met Val Ile Val Pro Lys Asp Asp Arg Ile 3045 3050 3055 Asp Pro Ser Arg Leu His His Trp Ile Glu Gln Glu Arg Val Thr Ile 3060 3065 3070 Phe Glu Ser Thr Pro Ala Leu Ile Val Pro Phe Leu Glu Tyr Val His 3075 3080 3085 Glu His Arg Leu Asp Met Ser Trp Met Glu Leu Leu Ile Thr Ser Ser 3090 3095 3100 Asp Ser Cys Ser Val Ala Asp Tyr Arg Thr Leu Gln Glu Arg Phe Gly 3105 3110 3115 3120 Ser Leu Phe Arg Ile Val Asn Ala Tyr Gly Val Thr Glu Ala Ala Ile 3125 3130 3135 Asp Ser Ser Phe Tyr Asp Glu Pro Leu Ala Lys Leu Pro Gln Thr Gly 3140 3145 3150 His Val Pro Ile Gly Lys Ala Trp Leu Asn Ala Lys Phe Tyr Ile Val 3155 3160 3165 Asp Ala His Leu Asn Pro Val Pro Val Gly Val Leu Gly Glu Leu Val 3170 3175 3180 Ile Gly Gly Val Gly Val Ala Arg Gly Tyr Leu Asn Arg Pro Glu Leu 3185 3190 3195 3200 Thr Glu Glu Lys Phe Val Asp Ser Pro Phe Ala Ala Gly Glu Arg Leu 3205 3210 3215 Tyr Arg Thr Gly Asp Leu Ala Arg Trp Met Glu Asp Gly Asn Val Asp 3220 3225 3230 Phe Ile Gly Arg Ile Asp Asn Gln Ala Lys Ile Arg Gly Tyr Arg Ile 3235 3240 3245 Glu Thr Gly Glu Ile Glu Ser Gln Leu Leu Arg Val Lys Gly Val Arg 3250 3255 3260 Glu Ala Val Val Leu Val Leu Ser Asp Ala Asn Gly Gln Lys Ala Leu 3265 3270 3275 3280 Cys Ala Tyr Tyr Thr Pro Asp Thr Gly Ala Glu Leu Ala Val Asn Asp 3285 3290 3295 Leu Arg Ser Thr Leu Ala Gln Glu Leu Pro Gly Tyr Met Ile Pro Ser 3300 3305 3310 Tyr Phe Val Glu Leu Glu His Leu Pro Leu Thr Pro Asn Gly Lys Ile 3315 3320 3325 Asp Arg Lys Ala Leu Pro Ala Pro Glu Gly Glu Ala Gly Asn Gly Thr 3330 3335 3340 Glu Tyr Val Ala Pro Arg Asn Glu Leu Glu Thr Lys Leu Ala Ala Ile 3345 3350 3355 3360 Trp Gln Glu Val Leu Gly Leu Ala Lys Glu Ile Gly Val His Asp Asn 3365 3370 3375 Phe Phe Asp Ile Gly Gly His Ser Leu Arg Ala Thr Thr Leu Val Ser 3380 3385 3390 Lys Ile His Lys Glu Leu Asn Val Asp Leu Pro Leu Arg Asp Val Phe 3395 3400 3405 Arg His Ser Thr Ile Glu Ser Met Ala Ala Ala Ile Ser Arg Leu Asp 3410 3415 3420 Glu Gln Thr Phe Val Ser Ile Pro Val Ala Asp Asp Arg Glu Val Tyr 3425 3430 3435 3440 Pro Gln Ser Phe Ala Gln Lys Arg Leu Phe Ile Leu Asn Gln Leu Glu 3445 3450 3455 Gly Ala Glu Leu Ser Tyr Asn Met Pro Glu Ala Met Leu Leu Glu Gly 3460 3465 3470 Ala Leu Asp Arg Ala Arg Phe Glu Glu Ala Phe Arg Lys Leu Val Thr 3475 3480 3485 Arg His Glu Thr Leu Arg Thr Gly Phe Glu Met Val Asp Gly Glu Ala 3490 3495 3500 Ser Gln Arg Ile Tyr Gln Asp Val Asn Val Ala Val Glu Phe Tyr Arg 3505 3510 3515 3520 Ala Asp Lys Gln Glu Ala Glu Glu Thr Val Arg Arg Phe Val Arg Pro 3525 3530 3535 Phe Asp Leu Ala Lys Pro Pro Leu Leu Arg Val Gly Leu Val Glu Leu 3540 3545 3550 Ala Pro Glu Arg His Ile Leu Met Tyr Asp Met His His Ile Ile Ser 3555 3560 3565 Asp Gly Val Ser Met Glu Ile Phe Val Glu Glu Phe Val Arg Leu Tyr 3570 3575 3580 Gly Gly Glu Gln Leu Asp Pro Leu Arg Ile Gln Tyr Lys Asp Tyr Thr 3585 3590 3595 3600 Val Trp Gln His Ser Gln Glu Gln Lys Glu Arg Leu Gln Arg Gln Glu 3605 3610 3615 Ala Tyr Trp Leu Asp Met Phe Gln Gly Glu Leu Pro Val Leu Glu Met 3620 3625 3630 Pro Thr Asp Tyr Pro Arg Pro Ala Val Gln Ser Tyr Glu Gly Gln Thr 3635 3640 3645 Leu Glu Phe Phe Phe Asp Ala Ser Lys Thr Asp Gly Leu Lys Gln Leu 3650 3655 3660 Ala Ser Glu Thr Gly Thr Thr Leu Phe Met Val Leu Leu Ala Ala Tyr 3665 3670 3675 3680 Asn Val Leu Leu His Lys Tyr Ser Gly Gln Glu Asp Val Ile Val Gly 3685 3690 3695 Thr Pro Ile Ala Gly Arg Asn His Gly Asp Val Gln Pro Leu Ile Gly 3700 3705 3710 Ile Phe Leu Asn Thr Leu Ala Ile Arg Ser Tyr Pro Ala Ser Glu Lys 3715 3720 3725 Thr Phe Leu Ser Tyr Leu Asn Glu Val Lys Glu Thr Thr Leu His Ala 3730 3735 3740 Phe Glu His Gln Asn Tyr Pro Phe Glu Glu Leu Val Asp Lys Val Gln 3745 3750 3755 3760 Val Thr Arg Asp Leu Ser Arg Asn Pro Leu Phe Asp Thr Leu Phe Thr 3765 3770 3775 Met Gln Asn Thr Glu Asn Glu Glu Phe Glu Leu Glu Gly Leu Arg Leu 3780 3785 3790 Ile Pro Tyr Pro Ser Ala Leu Asp Thr Ala Lys Phe Asp Ile Ser Leu 3795 3800 3805 Asp Val Gly Glu Glu Asn Gly Gly Leu Asp Tyr Ser Phe Glu Tyr Ala 3810 3815 3820 Thr Ala Leu Tyr Lys Arg Glu Thr Ile Glu Arg Leu Ala Lys His Tyr 3825 3830 3835 3840 Glu Gln Leu Leu Val Thr Ile Val Ser Arg Pro Asp Ala Lys Ile Ala 3845 3850 3855 Glu Leu Asn Leu Leu Thr Ala Glu Glu Lys Glu Gln Ile Leu Gly Ala 3860 3865 3870 Phe Asn Pro Ala Gln Pro Glu Ala Thr Ser Ala Ala Ala Phe His Arg 3875 3880 3885 Leu Phe Glu Glu Gln Ala Glu Arg Thr Pro Glu Thr Ala Ala Val Val 3890 3895 3900 Tyr Glu Asn Asp Arg Leu Thr Tyr Ala Glu Leu Asn Glu Arg Ala Asn 3905 3910 3915 3920 Arg Leu Ala Ala Thr Leu Arg Thr Ser Gly Ile Gly Arg Glu Thr Ile 3925 3930 3935 Val Gly Ile Leu Ala Glu Arg Ser Val Asp Leu Leu Val Ala Val Leu 3940 3945 3950 Ala Val Trp Lys Ala Gly Gly Ala Tyr Val Pro Leu Asp Pro Asp Tyr 3955 3960 3965 Pro Ala Glu Arg Val Arg Phe Met Leu Glu Asp Ser Gly Ala Lys Val 3970 3975 3980 Leu Leu Thr Gln Thr Ala Leu Arg Glu Arg Ala Glu Ala Trp Leu Gly 3985 3990 3995 4000 Glu Glu Glu Leu Ala Leu Ala Ala Val Leu Tyr Leu Asp Asp Glu Thr 4005 4010 4015 Ser Tyr Ser Glu Glu Arg Ala Asn Ala Pro Ile Val Ser Gly Met Val 4020 4025 4030 Ser Gly Lys Leu Thr Asp Ala Val Asp Asp Gly Asp Glu Ser His Gln 4035 4040 4045 Asn Val Gly Thr Asp Ser Phe His Glu Ala Arg Pro Glu Asp Leu Ala 4050 4055 4060 Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly Lys Pro Lys Gly Val Met 4065 4070 4075 4080 Ile Glu His Arg Ser Leu Val Asn Thr Ala Ala Gly Tyr Arg Arg Lys 4085 4090 4095 Tyr Arg Leu Asp Gln Phe Pro Val Arg Leu Leu Gln Leu Ala Ser Phe 4100 4105 4110 Ser Phe Asp Val Phe Val Gly Asp Ile Ala Arg Thr Leu Tyr Asn Gly 4115 4120 4125 Gly Thr Met Val Ile Val Pro Lys Asp Asp Arg Ile Asp Pro Ser Arg 4130 4135 4140 Leu His His Trp Ile Glu Gln Glu Arg Val Thr Ile Phe Glu Ser Thr 4145 4150 4155 4160 Pro Ala Leu Ile Val Pro Phe Leu Glu Tyr Val His Glu His Arg Leu 4165 4170 4175 Asp Met Ser Trp Met Glu Leu Leu Ile Thr Asn Ser Asp Ser Cys Ser 4180 4185 4190 Val Ala Asp Tyr Arg Thr Leu Gln Glu Arg Phe Gly Ser Leu Phe Arg 4195 4200 4205 Ile Val Asn Ala Tyr Gly Val Thr Glu Ala Ala Ile Asp Ser Ser Phe 4210 4215 4220 Tyr Asp Glu Glu Leu Ala Lys Leu Pro Gln Thr Gly Asn Val Pro Ile 4225 4230 4235 4240 Gly Lys Ala Trp Leu Asn Ala Lys Phe Tyr Ile Val Asp Ala His Leu 4245 4250 4255 Asn Pro Val Pro Val Gly Val Leu Gly Glu Leu Val Ile Gly Gly Ile 4260 4265 4270 Gly Val Ala Arg Gly Tyr Leu Asn Arg Pro Glu Leu Thr Glu Glu Lys 4275 4280 4285 Phe Val Asp Ser Pro Phe Ala Ala Gly Glu Arg Leu Tyr Arg Thr Gly 4290 4295 4300 Asp Leu Ala Arg Trp Met Glu Asp Gly Asn Val Asp Phe Ile Gly Arg 4305 4310 4315 4320 Ile Asp Asn Gln Ala Lys Ile Arg Gly Tyr Arg Ile Glu Thr Gly Glu 4325 4330 4335 Ile Glu Ser Gln Leu Leu Arg Val Glu Gly Val Arg Glu Ala Val Val 4340 4345 4350 Leu Val Arg Ser Asp Ala Asn Gly Gln Lys Ala Leu Cys Ala Tyr Tyr 4355 4360 4365 Thr Leu Asp Thr Gly Ala Glu Leu Ala Val Asn Asp Leu Arg Ser Ala 4370 4375 4380 Leu Ala Gln Glu Leu Pro Gly Tyr Met Ile Pro Ser Tyr Phe Val Glu 4385 4390 4395 4400 Leu Gly Arg Leu Pro Leu Thr Pro Asn Gly Lys Ile Asp Arg Lys Ala 4405 4410 4415 Leu Pro Ala Pro Glu Gly Glu Ala Gly Ser Gly Thr Glu Tyr Val Ala 4420 4425 4430 Pro Arg Asn Glu Leu Glu Thr Lys Leu Ala Ala Ile Trp Gln Glu Val 4435 4440 4445 Leu Gly Leu Ala Lys Glu Ile Gly Val His Asp Asn Phe Phe Asp Ile 4450 4455 4460 Gly Gly His Ser Leu Arg Ala Thr Thr Leu Ala Gly Lys Val Phe Lys 4465 4470 4475 4480 Glu Leu Asn Val Asn Leu Pro Leu Arg Asp Val Phe Arg His Ser Thr 4485 4490 4495 Ile Ala Ala Met Ala Glu Ala Ile Ala Arg Met Glu Arg Arg Glu His 4500 4505 4510 Glu Ala Ile Pro Gln Ala Glu Glu Arg Glu Tyr Tyr Pro Leu Ser Ser 4515 4520 4525 Ala Gln Lys Arg Leu Phe Ile Gln His Thr Leu Asp Gly Ala Asp Gln 4530 4535 4540 Leu Tyr Asn Met Pro Glu Leu Val Gln Val Glu Gly Glu Phe Asp Leu 4545 4550 4555 4560 Asp Arg Leu Glu Ala Ala Leu Arg Lys Leu Ile Thr Arg His Glu Ser 4565 4570 4575 Leu Arg Thr Gly Phe Glu Ile Val Lys Gly Glu Ala Val Gln Arg Val 4580 4585 4590 Tyr Pro Gln Val Asp Phe Ala Ile Glu His His Gln Ala Asp Lys Glu 4595 4600 4605 Asp Ala Ala Gln Ile Glu Gln Ile Val Arg Ser Phe Ile Arg Pro Phe 4610 4615 4620 Asp Leu Gly Lys Pro Pro Leu Leu Arg Ala Gly Val Ile Glu Leu Glu 4625 4630 4635 4640 Pro Asn Leu His Ile Leu Leu Phe Asp Met His His Met Val Ser Asp 4645 4650 4655 Gly Val Ser Met Ala Ile Val Ile Asp Glu Phe Ser Ser Phe Tyr Ala 4660 4665 4670 Gly Glu Glu Leu Pro Pro Leu Arg Ile Gln Tyr Lys Asp Tyr Ala Val 4675 4680 4685 Trp Gln Gln Ser Lys Ala His Arg Glu Arg Ile Gly Arg Gln Glu Ala 4690 4695 4700 Tyr Trp Leu Gln Thr Phe Glu Gly Glu Leu Pro Thr Ala Asp Leu Pro 4705 4710 4715 4720 Ile Asp Tyr Glu Arg Ser Ala Ala Arg Ser Tyr Glu Gly Ala His Leu 4725 4730 4735 Glu Phe Asn Val Glu Ala Ser Leu Ser Ala Arg Leu Arg Glu Leu Ala 4740 4745 4750 Ala Glu Arg Glu Ser Thr Leu Phe Met Val Leu Leu Ala Ala Tyr Thr 4755 4760 4765 Val Leu Leu Ser Lys Tyr Ser Gly Gln Glu Asp Leu Ile Val Gly Thr 4770 4775 4780 Pro Val Ala Gly Arg Thr Asn Ala Asp Leu Glu Ser Val Ile Gly Met 4785 4790 4795 4800 Phe Val Asn Thr Leu Ala Ile Arg Asn Arg Pro Ser Gly Asp Lys Thr 4805 4810 4815 Phe Leu Ser Tyr Leu Glu Glu Val Lys Glu Thr Ala Leu Gly Ala Phe 4820 4825 4830 Glu Asn Gln Asp Tyr Pro Phe Glu Glu Leu Val Glu Arg Leu Asn Val 4835 4840 4845 Lys Arg Glu Pro Gly Arg Phe Pro Leu Phe Asp Ala Val Phe Asp Leu 4850 4855 4860 Gln Asn Ile Glu Glu Arg Asp Ala Glu Leu Glu Gly Val Ser Leu Lys 4865 4870 4875 4880 Thr Tyr Glu Leu Asp His Leu Glu Glu Ala Lys Phe Asp Leu Thr Leu 4885 4890 4895 Phe Met Tyr Glu Asn Asn Gly Ala Leu Ser Gly Gly Phe Phe Tyr Ala 4900 4905 4910 Thr Lys Leu Phe Lys Glu Ala Met Ile Arg Thr Leu Thr Glu Asp Tyr 4915 4920 4925 Leu Arg Val Leu Ser Gln Ile Ala Glu Asn Pro Gln Leu Glu Leu Ser 4930 4935 4940 Arg Ile Glu Cys His Lys Pro Ala Ala Gly Ala Lys Ser Ala Val Asp 4945 4950 4955 4960 Thr Ile Glu Phe Ala Phe 4965 <210> 5 <211> 1102 <212> PRT <213> Paenibacillus polymyxa ATCC21830 <400> 5 Met Lys Ser Leu Phe Glu Lys Glu Glu Arg Tyr Trp Ser Gly Lys Phe 1 5 10 15 Asp Ala Asp Asp Ser Leu Ser Phe Leu Pro Tyr Ser Gln Ser Ser Lys 20 25 30 Leu Ser Ala Asp Gly Glu Ala Ala Ala Glu Pro Gly Leu Leu His Arg 35 40 45 Thr Leu Pro Ser Glu Ile Ser Glu Arg Ile Ile Cys Leu Ala Asn Gly 50 55 60 Ser Asp Leu Ala Leu Tyr Met Ile Val Leu Ala Gly Val Lys Ser Leu 65 70 75 80 Leu Phe Lys Tyr Thr Gly Arg Asp Gln Val Leu Val Gly Met Pro Ser 85 90 95 Tyr Ser Ala Asp Pro Asp Gly Thr Pro Pro Pro His Asp Ile Leu Val 100 105 110 Ile Lys Thr Ser Val Ser Arg Glu Thr Thr Leu Arg Thr Leu Leu Gly 115 120 125 Gly Ile Lys Ala Ser Ile Gly Glu Ala Leu Glu His Gln His Leu Pro 130 135 140 Phe Arg Lys Met Val Glu Pro Leu His Leu Asp Tyr Thr Gly Asp Gly 145 150 155 160 Leu Pro Val Val Asn Thr Val Ala Ser Phe Ala Pro Ile His Pro Glu 165 170 175 Pro Leu Gly Asn Arg Val Ala Ala Asp Thr Val Phe Arg Phe Asp Arg 180 185 190 Gln Asn His Ser Ile Glu Leu Glu Ile Ser Phe Asp Gly Gln Arg Tyr 195 200 205 Glu Arg Ala Phe Val Glu Gln Ala Ala Asp His Leu Val Gln Leu Leu 210 215 220 Ser Val Leu Leu Phe Gln Pro Asp Leu Glu Leu Gly Gln Ala Asp Val 225 230 235 240 Leu Ser Pro Asp Glu Arg Glu Thr Leu Leu Lys Arg Phe Asn Asp Thr 245 250 255 Glu Thr Gly Phe Glu Arg Gly Lys Thr Ile Tyr Gly Leu Phe Glu Glu 260 265 270 Gln Ala Glu Leu Tyr Pro Asp Asn Val Ala Ala Val Met Asn Glu Arg 275 280 285 Gln Leu Thr Tyr Arg Glu Leu Asn Glu Arg Ser Asn Arg Leu Ala Arg 290 295 300 Lys Leu Arg Glu Thr Gly Val Glu Ala Asp Gln Leu Val Ala Ile Leu 305 310 315 320 Ala Glu Arg Ser Leu Asp Met Val Val Gly Ile Leu Ala Ile Leu Lys 325 330 335 Ala Gly Gly Ala Tyr Val Pro Val Asp Pro Asp Tyr Pro Glu Glu Arg 340 345 350 Ile Arg Phe Met Ile Glu Asp Ser Gly Ala Pro Leu Leu Leu Ile Gln 355 360 365 Lys His Leu His Glu Lys Thr Asp Phe Ala Gly Thr Arg Leu Glu Leu 370 375 380 Asp Asp Phe Val Trp Gly Asp Arg Gly Ala Asp Ser Glu Gly Ala Leu 385 390 395 400 Asp Ala Ser Asn Leu Glu Pro Ile Ser Gly Pro Val Asn Leu Ala Tyr 405 410 415 Val Ile Tyr Thr Ser Gly Thr Thr Gly Arg Pro Lys Gly Thr Leu Ile 420 425 430 Glu His Lys Asn Val Val Arg Leu Leu Phe Asn Asp Lys Asn Leu Phe 435 440 445 Asp Phe Gly Pro Ser Asp Thr Trp Thr Leu Phe His Ser Phe Cys Phe 450 455 460 Asp Phe Ser Val Trp Glu Met Tyr Gly Ala Leu Leu Asn Gly Gly Lys 465 470 475 480 Leu Val Ile Val Pro Pro Leu Thr Ala Lys Asn Pro Ala Asp Phe Leu 485 490 495 Ala Leu Leu Gly Arg Glu Gln Val Thr Ile Leu Asn Gln Thr Pro Thr 500 505 510 Tyr Phe Tyr Gln Leu Leu Arg Lys Val Leu Ala Asp His Pro Tyr Asp 515 520 525 Leu Arg Ile Arg Asn Val Ile Phe Gly Gly Glu Ala Leu Ser Pro Leu 530 535 540 Leu Leu Lys Gly Phe Lys Thr Lys Tyr Pro Glu Thr Lys Leu Ile Asn 545 550 555 560 Met Tyr Gly Ile Thr Glu Thr Thr Val His Val Thr Tyr Lys Glu Ile 565 570 575 Thr Trp Val Glu Met Glu Ala Ala Lys Ser Asn Ile Gly Lys Pro Ile 580 585 590 Pro Thr Leu Arg Val Tyr Val Leu Asp Glu Asn Arg Arg Pro Val Pro 595 600 605 Ile Gly Val Ala Gly Glu Met Tyr Val Ala Gly Ala Gly Leu Ala Arg 610 615 620 Gly Tyr Leu Asn Arg Pro Asp Leu Thr Ala Glu Lys Phe Val Asp Ser 625 630 635 640 Pro Phe Ala Glu Gly Glu Lys Leu Tyr Arg Ser Gly Asp Leu Ala Ala 645 650 655 Trp Leu Pro Asp Gly Asn Ile Glu Tyr Leu Gly Arg Ile Asp His Gln 660 665 670 Val Lys Ile Arg Gly Tyr Arg Ile Glu Leu Asp Glu Ile Glu Thr Gln 675 680 685 Leu Leu Lys Ile Ala Ala Val Gln Glu Ala Lys Val Leu Asp Arg Asp 690 695 700 Asp Ala Asn Gly Gln Lys Gln Leu Val Ala Tyr Tyr Val Ala Glu Thr 705 710 715 720 Arg Leu Ala Val His Glu Leu Lys Glu Glu Leu Ala Lys Gln Leu Pro 725 730 735 Gly Tyr Met Ile Pro Ser His Leu Val Gln Leu Pro Arg Met Pro Leu 740 745 750 Thr Pro Asn Gly Lys Ile Asp Arg Lys Ala Leu Pro Ala Pro Glu Glu 755 760 765 Ala Ala Ala Arg Gly Ala Glu Tyr Val Ala Pro Arg Thr Leu Leu Glu 770 775 780 Met Lys Ile Ala Arg Val Trp Gln Asp Thr Leu Gly Val Pro Gln Val 785 790 795 800 Gly Val Lys Asp Asn Phe Phe Glu Leu Gly Gly Asn Ser Leu Ser Leu 805 810 815 Met Arg Leu Val Gln Ala Val Tyr Asp Glu Thr Gly Ile Glu Ile Pro 820 825 830 Leu Asn Arg Gln Phe His Asn Val Thr Val Glu Ala Met Ala Phe Gly 835 840 845 Glu Gly Asp Leu Gly Leu Asp Lys Gly Gly Asp Ser Phe Ile Lys Leu 850 855 860 Asn Lys Val Gly Asn Leu Asn Val Phe Cys Phe Pro Pro Gly Ser Gly 865 870 875 880 Phe Gly Ile Gly Tyr Arg Glu Leu Ala Ser Arg Leu Asp Gly Arg Ser 885 890 895 Val Leu Tyr Ser Ile Asp Phe Ile Asp Asp Ala Val Asp Tyr Glu Ala 900 905 910 Met Leu Asn Arg Tyr Val Asp Glu Ile Val Arg Ile Gln Pro Glu Gly 915 920 925 Pro Tyr Val Leu Leu Gly Tyr Cys Phe Gly Gly Asn Leu Thr Phe Glu 930 935 940 Val Ala Lys Thr Met Glu Lys Arg Gly Tyr Ser Val Thr Asp Val Leu 945 950 955 960 Met Val Asp Ser Trp Ile Lys His Thr Leu Thr Pro Ser Glu Thr Ser 965 970 975 Glu Lys Glu Leu Glu Glu Thr Leu Ala Asp Phe Asp Glu Glu Glu Lys 980 985 990 Glu Leu Met Ser Asn Pro Leu Val Arg Glu Arg Val His Arg Lys Val 995 1000 1005 Lys Ala Thr Leu Ala Tyr Glu Ala Gln Leu Ile Asn Ser Gly Thr Ile 1010 1015 1020 Pro Ala Arg Ile Tyr Glu Leu Ile Ala Lys Asp Ser Glu Ala Phe Arg 1025 1030 1035 1040 Leu Glu His Gln Leu Pro Ser Trp Arg Gly Ala Thr Thr Gln Ala Tyr 1045 1050 1055 Ala Asp Tyr Arg Leu Glu Gly Ala His Glu Glu Leu Leu Glu Leu Ala 1060 1065 1070 Arg Val Asp Glu Thr Ala Val Val Ile Arg Asp Ile Leu Glu Gln Val 1075 1080 1085 Lys Arg Gln Ile Glu Ala Glu Ala Gly Val Leu His Gly Ser 1090 1095 1100 <210> 6 <211> 5789 <212> PRT <213> Paenibacillus polymyxa ATCC21830 <400> 6 Met Arg Glu Asp Thr Asn Glu Gln Tyr Gly Leu Thr Gln Ala Gln Arg 1 5 10 15 Arg Ile Trp Phe Met Glu Ile Met Asn Pro Gly Thr Ser Ile Thr Met 20 25 30 Leu Ser Ala Thr Tyr Gln Ile Thr Gly Glu Ile Asn Thr Gln Leu Leu 35 40 45 Lys Gln Ala Ala Ala Glu Ile Val Lys Thr Tyr Asp Val Phe Arg Ile 50 55 60 Arg Ile Ser Gly Asp Leu Gln Asn Pro Thr Gln Trp Phe Glu Glu Pro 65 70 75 80 Glu Asn Val Gln Ala Arg Ile Ser Arg Leu Glu Ile Gly Thr Thr Glu 85 90 95 Gln Phe Tyr Ala Trp Val Lys Glu Val Ser Glu Lys Pro Ala Ser Val 100 105 110 Phe Asp Glu His Leu Tyr Gln Phe Thr Ile Ile His Phe Ala Asn Gly 115 120 125 Gln Val Trp Leu Asn Leu Thr Val Asn His Ile Ile Ala Asp Gly Leu 130 135 140 Ser Val Asn Ala Leu Leu His Ala Val Met Glu Lys Tyr Leu Glu Leu 145 150 155 160 Arg Lys Gly Ile Ser Ser Ser Tyr Gln Pro Pro Ser Tyr Leu Asp Tyr 165 170 175 Ile Ser Ser Glu Arg Glu Tyr Glu Gln Ser Gln Arg Tyr Gln Lys Gly 180 185 190 Lys Glu Tyr Trp Leu Thr Lys Tyr Asn Thr Leu Pro Glu Thr Thr Gly 195 200 205 Ile Lys Ser Tyr Pro Pro Phe Ser Ile Gly Ser Glu Ser Asn Lys Leu 210 215 220 Ser Ile Thr Leu Asp Gly Ser Arg Tyr Glu Arg Ile Leu Ala Phe Cys 225 230 235 240 Glu Gln Tyr Gln Val Ser Leu Tyr Thr Leu Phe Leu Ser Ala Met Tyr 245 250 255 Ala Leu Leu Tyr Lys Leu Thr Asp Ser Thr Asp Val Pro Val Gly Thr 260 265 270 Val Phe Ala Asn Arg Thr Ser Lys Lys Glu Lys Glu Thr Ile Gly Met 275 280 285 Phe Val Ser Thr Val Ala Thr Arg Ile Cys Leu Asn Pro Asp Gly His 290 295 300 Val Leu Ser Leu Ile Gln Thr Val Ser Lys Glu Asn Thr Ala Asp Leu 305 310 315 320 Arg Tyr Gln Lys Tyr Pro Tyr Asn Gln Leu Ile Gln Asp Leu Arg Glu 325 330 335 Gln His Gly Arg Asn Asp Leu Ser Gly Leu Phe Arg Thr Ser Leu Glu 340 345 350 Tyr Leu Pro Leu Lys Ile Val Glu Tyr Glu Glu Ile Lys Val Arg Leu 355 360 365 Glu Ala His Phe Ala Arg His Glu Met Asp Asp Leu Leu Leu Arg Phe 370 375 380 Asp His Met Leu Asn Glu Ser His Val Ile Leu His Ala Ser Tyr Arg 385 390 395 400 Thr Gly Leu Phe Glu Lys Ala Glu Ile Asp Arg Ile Met Glu Gln Tyr 405 410 415 Val Thr Val Leu Asp Gln Phe Leu Gln Thr Pro Glu Leu Pro Val Arg 420 425 430 Glu Ile Ser Leu Leu Ser Asp Asp Glu Lys His Arg Ile Leu Asn Val 435 440 445 Phe Asn Pro Pro Val Ala Gly Leu Ser Glu Gly Glu Ala Phe His Arg 450 455 460 Tyr Val Glu Lys Phe Ala Arg Glu Ile Pro Asp His Pro Ala Val Val 465 470 475 480 Tyr Met Asp Lys Gln Leu Thr Tyr Ser Glu Leu Asn Glu Arg Ala Glu 485 490 495 Arg Leu Ala Ser Leu Leu Arg Glu Gln Gly Val Gly Arg Glu Thr Ile 500 505 510 Thr Gly Ile Trp Ala Glu Arg Ser Val Glu Leu Leu Val Gly Val Leu 515 520 525 Ala Val Trp Lys Ala Gly Gly Ala Tyr Val Pro Leu Asp Pro Asp Tyr 530 535 540 Pro Ala Glu Arg Ile Glu Tyr Met Leu Ser Asp Ser Asp Ala Ser Val 545 550 555 560 Leu Leu Thr Gln Arg His Leu Leu Glu Arg Ala Gly Gly Trp Leu Ala 565 570 575 Asp Asp Arg Leu Lys Leu Gln Ala Val Tyr Ala Met Asp Asp Glu Gln 580 585 590 Ile Tyr Asn Gly Asp Ala Leu Ala Val Glu Phe Glu Ser Ala Asp Ser 595 600 605 Ala Pro Gln Asp Leu Ala Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly 610 615 620 Arg Pro Lys Gly Val Met Ile Glu His Gly Ser Leu Val Asn Thr Ala 625 630 635 640 Asp Ala Tyr Arg Arg Glu Tyr Arg Leu Asp Gln Phe Pro Val Arg Leu 645 650 655 Leu Gln Leu Ala Ser Phe Ser Phe Asp Val Phe Val Gly Asp Ile Ala 660 665 670 Arg Thr Leu Tyr Asn Gly Gly Thr Met Val Ile Val Pro Lys Asp Asp 675 680 685 Arg Ile Asp Pro Asn Leu Leu Tyr Gly Trp Ile Arg Asp Gln Asn Ile 690 695 700 Thr Val Phe Glu Ser Thr Pro Ala Leu Ile Leu Pro Phe Met Gln His 705 710 715 720 Ile Tyr Glu Glu Gly Leu Asp Val Ser Ser Met Gln Leu Leu Ile Thr 725 730 735 Ser Ser Asp Ala Cys Ser Val Thr Asp Tyr Arg Leu Leu Gln Glu Arg 740 745 750 Phe Gly Gly Gln Phe Arg Ile Ile Asn Ser Tyr Gly Val Thr Glu Ala 755 760 765 Ala Ile Asp Ser Ser Phe Tyr Gly Glu Pro Leu Asp Lys Leu Pro Pro 770 775 780 Ser Gly His Val Pro Ile Gly Lys Ala Trp Leu Asn Ala Arg Phe Tyr 785 790 795 800 Ile Val Asp Ala Ala Leu Lys Pro Val Pro Val Gly Val Pro Gly Glu 805 810 815 Leu Val Ile Gly Gly Ala Gly Val Ala Arg Gly Tyr Trp Asn Arg Pro 820 825 830 Asp Leu Thr Ala Glu Lys Phe Ala Asp Ser Pro Phe Val Pro Gly Glu 835 840 845 Arg Leu Tyr Arg Thr Gly Asp Leu Ala Arg Trp Leu Glu Asp Gly Asn 850 855 860 Val Asp Phe Ile Gly Arg Ile Asp Tyr Gln Val Lys Ile Arg Gly Phe 865 870 875 880 Arg Ile Glu Leu Gly Glu Ile Glu Thr Ala Leu Leu Arg Phe Pro Gly 885 890 895 Val Lys Gln Ala Val Val Thr Asp Arg Thr Asp Glu Gln Gly Gln Lys 900 905 910 Tyr Leu Cys Gly Tyr Val Val Glu Asp Ala Ser Val Gln Leu Ser Asp 915 920 925 Leu Leu Ser Gln Leu Lys Gln Glu Leu Pro Ala His Met Val Pro Ala 930 935 940 Arg Leu Val Ser Leu Asp Lys Leu Pro Leu Thr Pro Asn Gly Lys Ile 945 950 955 960 Asp Arg Lys Ala Leu Pro Glu Pro Thr Gly Glu Val Glu Ala Gly His 965 970 975 Glu Tyr Val Ala Pro Arg Thr Thr Leu Glu Thr Arg Leu Ala Leu Ile 980 985 990 Trp Gln Gln Val Leu Gly Ile Ala Arg Val Gly Val Gln Asp Asp Phe 995 1000 1005 Phe Asp Leu Gly Gly His Ser Leu Arg Ala Ser Thr Leu Val Ser Lys 1010 1015 1020 Ile Arg Lys Glu Leu Gln Val Glu Val Pro Leu Arg Glu Val Phe Arg 1025 1030 1035 1040 Tyr Thr Thr Ile Glu Gln Leu Ala Gln Arg Ile Gly Gly Leu Arg Gln 1045 1050 1055 Gln Glu Thr Tyr Glu Ile Thr Lys Ala Ala Glu Ala Glu Tyr Tyr Pro 1060 1065 1070 Val Ser Ser Glu Gln Lys Arg Leu Tyr Val Leu Arg Gln Leu Asp Gly 1075 1080 1085 Ala Glu Arg Ser Tyr Asn Met Ser Ala Ala Leu Leu Leu Glu Gly Lys 1090 1095 1100 Leu Asp Arg Thr Arg Val Glu His Ala Phe Arg Ala Leu Ile Gln Arg 1105 1110 1115 1120 His Glu Thr Leu Arg Thr Gly Ile Glu Gln Val Gln Gly Glu Leu Val 1125 1130 1135 Gln Arg Ile Tyr Asp Glu Leu Asp Phe Ala Val Asp Tyr Phe Gln Ala 1140 1145 1150 Ser Glu Arg Glu Val Glu Gln Val Val Glu Ala Tyr Tyr His Pro Phe 1155 1160 1165 Asp Leu Thr Lys Pro Pro Leu Leu Arg Ile Gly Leu Ile Glu Val Ala 1170 1175 1180 Glu Asp Arg His Ile Leu Leu Phe Asp Met His His Ile Val Ser Asp 1185 1190 1195 1200 Gly Ile Ser Thr Ala Leu Leu Phe Asp Glu Phe Ser Arg Leu Tyr Arg 1205 1210 1215 Gly Glu Glu Leu Ala Pro Leu Arg Ile Gln Tyr Lys Asp Tyr Ala Val 1220 1225 1230 Trp Gln His Ser Glu Ala Tyr Gly Gln Met Leu Gln Pro Gln Lys Glu 1235 1240 1245 Tyr Trp Leu Glu Gln Leu Ser Gly Glu Leu Pro Val Leu Glu Leu Pro 1250 1255 1260 Thr Asp Phe Pro Arg Pro Ala Val Gln Ser Phe Asp Gly Arg Thr Val 1265 1270 1275 1280 Lys Phe Tyr Ile Glu Lys Asp Arg Thr Glu Lys Leu Lys Glu Leu Ala 1285 1290 1295 Ala Arg Thr Gly Thr Thr Leu Tyr Met Val Leu Leu Ser Ala Tyr Thr 1300 1305 1310 Ile Leu Met His Lys Tyr Ser Gly Gln Glu Asp Leu Ile Val Gly Thr 1315 1320 1325 Pro Ile Ala Gly Arg Thr Gln Glu Glu Val Gln Pro Ile Val Gly Met 1330 1335 1340 Phe Ile Asn Thr Leu Ala Ile Arg Ser Arg Pro Glu Arg Ser Lys Pro 1345 1350 1355 1360 Tyr Leu Ser Tyr Leu Glu Glu Ile Lys Asp Ile Thr Leu Gly Ala Phe 1365 1370 1375 Glu His Gln Asn Tyr Leu Phe Glu Asp Leu Val Glu Ser Leu His Ile 1380 1385 1390 Pro Arg Ala Thr Gly Arg Asn Pro Leu Phe Asp Thr Phe Phe Ser Leu 1395 1400 1405 Gln Asn Thr Glu Asn Glu Gln Ile Val Ile Glu Gly Leu Glu Gln Ser 1410 1415 1420 Phe Tyr Pro Leu Glu Asn Gln Thr Ser Lys Phe Glu Leu Leu Leu Asp 1425 1430 1435 1440 Ile Ser Glu Leu Asp Gly Gln Leu Glu Cys Arg Leu Glu Tyr Ala Thr 1445 1450 1455 Ala Leu Tyr Lys Gln Glu Thr Ala Glu Arg Phe Ala Arg His Tyr Asp 1460 1465 1470 Lys Leu Leu Glu Thr Ile Ala Ala Ala Pro Asp Gly Asp Ile Ala Ser 1475 1480 1485 Leu Glu Met Leu Thr Glu Glu Glu Ile Arg Glu Leu Met Arg Gly Phe 1490 1495 1500 Asn Asp Ser Glu Ala Asp Tyr Pro Arg Gln Gln Thr Ile His Gly Leu 1505 1510 1515 1520 Phe Glu Glu Gln Ala Glu Leu Tyr Pro Asp Asn Val Ala Ala Val Met 1525 1530 1535 Asn Glu Arg Gln Leu Thr Tyr Arg Glu Leu Asn Glu Arg Ser Asn Arg 1540 1545 1550 Leu Ala Arg Lys Leu Arg Glu Thr Gly Val Glu Ala Asp Gln Leu Val 1555 1560 1565 Ala Ile Leu Ala Glu Arg Ser Leu Asp Met Val Val Gly Ile Leu Ala 1570 1575 1580 Ile Leu Lys Ala Gly Gly Ala Tyr Val Pro Val Asp Pro Asp Tyr Pro 1585 1590 1595 1600 Glu Glu Cys Ile Arg Phe Met Ile Glu Asp Ser Gly Ala Pro Leu Leu 1605 1610 1615 Leu Ile Gln Lys His Leu His Glu Lys Thr Asp Phe Val Gly Thr Arg 1620 1625 1630 Leu Glu Leu Asp Asp Phe Val Trp Gly Asp Arg Gly Ala Asp Ser Glu 1635 1640 1645 Gly Ala Leu Asp Ala Ser Asn Leu Glu Pro Ile Ser Gly Pro Leu Asn 1650 1655 1660 Leu Ala Tyr Val Ile Tyr Thr Ser Gly Thr Thr Gly Arg Pro Lys Gly 1665 1670 1675 1680 Thr Leu Ile Glu His Lys Asn Val Val Arg Leu Leu Phe Asn Asp Lys 1685 1690 1695 Asn Leu Phe Asp Phe Gly Pro Ser Asp Thr Trp Thr Leu Phe His Ser 1700 1705 1710 Phe Cys Phe Asp Phe Ser Val Trp Glu Met Tyr Gly Ala Leu Leu Tyr 1715 1720 1725 Gly Gly Lys Leu Val Ile Val Pro Pro Phe Thr Ala Lys Asn Pro Ala 1730 1735 1740 Asp Phe Leu Ala Leu Leu Gly Arg Glu Gln Val Thr Ile Leu Asn Gln 1745 1750 1755 1760 Thr Pro Thr Tyr Phe Tyr Gln Leu Leu Arg Glu Val Leu Thr Asp His 1765 1770 1775 Pro Tyr Asp Leu Arg Ile Arg Asn Val Ile Phe Gly Gly Glu Ala Leu 1780 1785 1790 Ser Pro Leu Leu Leu Lys Gly Phe Lys Thr Lys Tyr Pro Glu Thr Lys 1795 1800 1805 Leu Ile Asn Met Tyr Gly Ile Thr Glu Thr Thr Val His Val Thr Tyr 1810 1815 1820 Lys Glu Ile Thr Trp Val Glu Met Glu Ala Ala Lys Ser Asn Ile Gly 1825 1830 1835 1840 Lys Pro Ile Pro Thr Leu Arg Val Tyr Val Leu Asp Glu Asn Arg Arg 1845 1850 1855 Pro Val Pro Ile Gly Val Ala Gly Glu Met Tyr Val Ala Gly Glu Gly 1860 1865 1870 Leu Ala Arg Gly Tyr Leu Asn Arg Pro Asp Leu Thr Ala Glu Lys Phe 1875 1880 1885 Val Asp Ser Pro Phe Ala Glu Gly Glu Lys Leu Tyr Arg Ser Gly Asp 1890 1895 1900 Leu Ala Ala Trp Leu Pro Asp Gly Asn Ile Glu Tyr Leu Gly Arg Ile 1905 1910 1915 1920 Asp His Gln Val Lys Ile Arg Gly Tyr Arg Ile Glu Leu Asp Glu Ile 1925 1930 1935 Glu Thr Gln Leu Leu Asn Val Glu Gly Val Glu Glu Ala Val Val Leu 1940 1945 1950 Ala Arg Gln Asp Gly Gly Gly Glu Lys Ala Leu Val Ala Tyr Phe Val 1955 1960 1965 Ala Asp Arg Thr Leu Thr Val Ser Glu Met Arg Thr Ser Leu Ala Gln 1970 1975 1980 Gly Met Pro Gly Tyr Met Ile Pro Ser Tyr Phe Val Gln Leu Glu Arg 1985 1990 1995 2000 Met Pro Leu Thr Ala Asn Gly Lys Val Asp Arg Lys Ala Leu Pro Glu 2005 2010 2015 Pro Gln Gly Ser Leu Gln Thr Gly Val Glu Tyr Val Ala Pro Arg Asn 2020 2025 2030 Arg Met Glu Ser Gln Leu Val Lys Ile Trp Glu Glu Val Leu Gly Tyr 2035 2040 2045 Ser Gly Ile Gly Val Leu Asp Asn Phe Phe Glu Leu Gly Gly His Ser 2050 2055 2060 Leu Arg Ala Thr Asn Leu Val Ser Lys Ile Gln Lys Glu Met Asn Val 2065 2070 2075 2080 Glu Leu Pro Leu Arg Asp Val Phe Arg Tyr Thr Thr Val Glu Ser Met 2085 2090 2095 Ala Gly Ala Ile Ala Ser Leu Glu Glu Thr Arg His Asn Ser Ile Pro 2100 2105 2110 Lys Ala Glu Glu Lys Ala Tyr Tyr Pro Val Ser Ser Ala Gln Lys Arg 2115 2120 2125 Leu Tyr Val Leu His Gln Leu Asp Gly Ser Glu Leu Asn Tyr Asn Leu 2130 2135 2140 Pro Ser Ala Leu Gln Leu Glu Gly Ala Leu Asn Glu Ala Lys Val Glu 2145 2150 2155 2160 Lys Ala Leu Thr Thr Leu Val Ala Arg His Asp Met Leu Arg Thr Gly 2165 2170 2175 Phe Glu Ile Val Asn Gly Glu Pro Val Gln Arg Ile His Pro Ile Val 2180 2185 2190 Ala Phe Lys Val Glu Lys Leu Gln Ala Ser Glu Asp Gln Val Ala Ala 2195 2200 2205 Ile Leu Glu Gly Phe Ile Gln Pro Phe Asp Leu Thr Gln Pro Pro Leu 2210 2215 2220 Leu Arg Ala Leu Leu Ile Glu Leu Glu Lys Glu Lys Phe Leu Leu Ala 2225 2230 2235 2240 Leu Asp Ile His His Ile Gly Ser Asp Gly Leu Ser Met Asp Val Leu 2245 2250 2255 Leu Arg Glu Phe Val Arg Leu Tyr Asn Gly Glu Glu Leu Pro Glu Leu 2260 2265 2270 Arg Ile Gln Tyr Lys Asp Tyr Ala Val Trp Gln Gln Ser Glu Glu Gln 2275 2280 2285 Arg Gln Arg Ile Lys Arg Gln Glu Glu Tyr Trp Arg Gly Val Phe Ser 2290 2295 2300 Ser Glu Leu Pro Val Leu Glu Leu Pro Leu Asp Phe Ser Arg Pro Ala 2305 2310 2315 2320 Val Gln Gln Phe Asp Gly Gln Thr Leu Thr Phe Thr Leu Asp Ala Glu 2325 2330 2335 Lys Ser Glu Ala Leu Lys Arg Leu Ala Gly Asp Ser Gly Ala Thr Leu 2340 2345 2350 Tyr Met Leu Leu Leu Ala Ala Tyr Ser Val Leu Leu His Lys Tyr Ala 2355 2360 2365 Gly Gln Glu Asp Ile Val Val Gly Thr Pro Ile Ala Ala Arg Ser His 2370 2375 2380 Ala Asp Leu Gln Pro Ile Ile Gly Met Phe Val Asn Thr Leu Ala Leu 2385 2390 2395 2400 Arg Leu Gly Pro Ala Ala Glu Arg Thr Phe Leu Asp Tyr Leu Gln Glu 2405 2410 2415 Val Lys Glu Thr Thr Leu Gly Ala Tyr Glu His Gln Asp Tyr Pro Phe 2420 2425 2430 Glu Glu Leu Val Glu Ala Leu Gln Val Ser Arg Asp Leu Ser Arg Asn 2435 2440 2445 Pro Leu Phe Asp Thr Met Phe Ser Leu Gln Lys His Glu Ser Leu Asp 2450 2455 2460 Leu Thr Leu Glu Gly Leu Gln Trp Ser Leu Phe Asp Ile Glu Glu Lys 2465 2470 2475 2480 Thr Ala Lys Phe Asp Leu Ser Phe Asp Ile Val Glu Ala Asp Asn Glu 2485 2490 2495 Leu Val Cys Lys Ile Glu Tyr Ala Thr Ser Leu Phe Arg Gln Glu Thr 2500 2505 2510 Val Val Arg Leu Ala Gly His Tyr Glu Gln Leu Leu Thr Ser Ile Leu 2515 2520 2525 Ala Gln Pro Gly Ala Arg Ile Ser Asp Leu Asp Val Leu Thr Asp Ser 2530 2535 2540 Glu Lys His Asp Leu Leu Val Gly Phe Asp Val Ser Ser Ser Ala Leu 2545 2550 2555 2560 Ala Lys Gln Pro Ala Ala Glu Gly Thr Gly Leu Glu Ala Asp Glu Ser 2565 2570 2575 Trp Arg Glu Arg Thr Phe His Glu Leu Phe Glu Glu Gln Ala Glu Arg 2580 2585 2590 Thr Pro Glu Ala Leu Ala Val Val Tyr Glu Asp Ser Lys Leu Thr Tyr 2595 2600 2605 Ala Glu Leu Asn Ala Lys Ala Asn Arg Leu Ala Tyr Ala Leu Arg Ala 2610 2615 2620 Arg Gly Val Lys Pro Glu Gln Val Val Gly Ile Leu Ala Asp Arg Ser 2625 2630 2635 2640 Ala Glu Leu Leu Ile Gly Val Leu Ala Val Trp Lys Ala Gly Gly Ala 2645 2650 2655 Tyr Val Pro Leu Asp Pro Asp Tyr Pro Ala Glu Arg Ile Glu Tyr Met 2660 2665 2670 Leu Ala Asp Ser Gly Ala Ser Val Leu Leu Thr Gln Thr Arg Leu Leu 2675 2680 2685 Glu Gln Ala Glu Ala Trp Arg Ser Asp Gly Ala Leu Val Leu Gln Thr 2690 2695 2700 Val Leu Ala Leu Asp Asp Val Ala Thr Tyr Ser Leu Gly Ala Ala Glu 2705 2710 2715 2720 Ala Ala Val Gly Val Gln Ala Leu Gly Glu Ala Gly Ala Glu Ala Glu 2725 2730 2735 Ala Leu Ala Gln Ala Ala Ala Ala Glu Thr Ser Ala Thr Ala Glu Ala 2740 2745 2750 Glu Lys Asn Val Leu Ala Ala Asp Leu Ala Ser Asn Pro Ala Asn Val 2755 2760 2765 Asn Lys Pro Arg Asp Leu Ala Tyr Val Ile Tyr Thr Ser Gly Thr Thr 2770 2775 2780 Gly Lys Pro Lys Gly Val Met Ile Glu His Arg Ser Leu Val Asn Thr 2785 2790 2795 2800 Ala Ala Gly Tyr Arg Arg Glu Tyr Arg Leu Asp Gln Phe Pro Val Arg 2805 2810 2815 Leu Leu Gln Leu Ala Ser Phe Ser Phe Asp Val Phe Val Gly Asp Ile 2820 2825 2830 Ala Arg Thr Leu Tyr Asn Gly Gly Thr Met Val Ile Val Pro Lys Asp 2835 2840 2845 Asp Arg Ile Asp Pro Ser Arg Leu His His Trp Met Glu Arg Glu Arg 2850 2855 2860 Val Thr Ile Phe Glu Ser Thr Pro Ala Leu Ile Val Pro Phe Leu Glu 2865 2870 2875 2880 Tyr Val His Glu Gln Arg Leu Asp Met Ser Gly Met Glu Leu Leu Ile 2885 2890 2895 Thr Ser Ser Asp Ser Cys Ser Val Ala Asp Tyr Arg Thr Leu Gln Glu 2900 2905 2910 Arg Phe Gly Ser Gln Phe Arg Ile Ile Asn Ser Tyr Gly Val Thr Glu 2915 2920 2925 Ala Ala Ile Asp Ser Ser Phe Tyr Asp Glu Pro Leu Glu Lys Leu Pro 2930 2935 2940 Lys Thr Gly Ser Val Pro Ile Gly Lys Ala Trp Leu Asn Ala Lys Phe 2945 2950 2955 2960 Tyr Ile Val Asp Ala Asn Leu Lys Pro Val Pro Ile Gly Val Leu Gly 2965 2970 2975 Glu Leu Val Ile Gly Gly Val Gly Val Ala Arg Gly Tyr Leu Asn Arg 2980 2985 2990 Pro Asp Leu Thr Ala Glu Lys Phe Val Asp Ser Pro Phe Ala Thr Gly 2995 3000 3005 Glu Arg Leu Tyr Arg Thr Gly Asp Leu Ala Arg Trp Met Ser Asp Gly 3010 3015 3020 Asn Val Asp Phe Ile Gly Arg Ile Asp Asn Gln Val Lys Ile Arg Gly 3025 3030 3035 3040 Tyr Arg Ile Glu Thr Gly Glu Val Glu Ala Lys Leu Leu Ser Val Gly 3045 3050 3055 Gly Val Lys Glu Ala Val Val Val Val Arg Glu Asp Gln Glu Gly Gln 3060 3065 3070 Lys Ala Leu Cys Ala Tyr Tyr Thr Ala Glu Glu Gly Leu Thr Ala Ala 3075 3080 3085 Asp Leu Lys Arg Ala Ile Ala Ser Glu Leu Pro Gly Tyr Met Ile Pro 3090 3095 3100 Ser Tyr Phe Val Glu Leu Glu Arg Leu Pro Leu Thr Pro Asn Gly Lys 3105 3110 3115 3120 Ile Asp Arg Lys Ala Leu Pro Ala Pro Glu Gly Gly Ala Gly Gly Arg 3125 3130 3135 Glu Tyr Val Ala Pro Arg Thr Glu Leu Glu Ala Lys Leu Ala Ala Ile 3140 3145 3150 Trp Gln Asp Val Leu Val Arg Glu Lys Ala Val Gly Val Thr Asp Asn 3155 3160 3165 Phe Phe Asp Leu Gly Gly His Ser Leu Arg Ala Thr Thr Leu Val Ser 3170 3175 3180 Lys Met His Lys Glu Leu Gly Val Glu Phe Pro Leu Arg Asp Val Phe 3185 3190 3195 3200 Arg Tyr Ser Thr Val Glu Glu Met Ala Ala Ala Met Glu Arg Leu Glu 3205 3210 3215 Ile Gly Ser Phe Thr Ala Ile Pro Ala Ala Glu Ser Ser Glu Tyr Tyr 3220 3225 3230 Pro Leu Ser Ser Ala Gln Lys Arg Leu Tyr Ile Leu Asn Gln Leu Glu 3235 3240 3245 Gly Ala Glu Leu Ser Tyr Asn Ile Pro Gly Ala Met Leu Leu Glu Gly 3250 3255 3260 Glu Leu Asp Arg Pro Arg Phe Glu Glu Ala Phe Arg Gly Leu Val Ala 3265 3270 3275 3280 Arg His Glu Thr Leu Arg Thr Gly Phe Glu Met Val Lys Gly Glu Ala 3285 3290 3295 Val Gln Arg Ile Tyr Glu Glu Ala Ala Phe Gln Val Glu Tyr Val Gln 3300 3305 3310 Ile Ser Glu Glu Gln Ala Glu Glu Thr Val Arg Gln Phe Val Arg Pro 3315 3320 3325 Phe Asp Leu Ala Lys Pro Pro Leu Leu Arg Val Gly Leu Ala Glu Leu 3330 3335 3340 Ala Pro Asp Arg His Ile Leu Met Phe Asp Thr His His Ile Val Ser 3345 3350 3355 3360 Asp Gly Val Ser Ile Asp Val Leu Ile Glu Glu Leu Val Arg Leu Tyr 3365 3370 3375 Ser Gly Glu Gln Leu Glu Pro Leu Arg Ile Gln Tyr Lys Asp Tyr Ala 3380 3385 3390 Val Trp Gln Gln Ser Asp Glu Gln Lys Ala Gln Leu Ala Lys Gln Glu 3395 3400 3405 Ala Tyr Trp Leu Asp Met Phe Arg Gly Glu Leu Pro Val Leu Glu Leu 3410 3415 3420 Pro Thr Asp Tyr Pro Arg Pro Ala Met Gln Ser Tyr Glu Gly Arg Thr 3425 3430 3435 3440 Leu Gln Leu Phe Met Asp Ser Glu Lys Ser Glu Gly Leu Lys Arg Leu 3445 3450 3455 Ala Ala Glu Asn Gly Ala Thr Leu Tyr Met Val Leu Leu Ala Gly Tyr 3460 3465 3470 Thr Ile Leu Leu His Lys Tyr Thr Gly Gln Glu Asp Val Val Val Gly 3475 3480 3485 Thr Pro Ile Ala Gly Arg Asn His Ser Asp Val Gln Pro Leu Ile Gly 3490 3495 3500 Met Phe Val Asn Thr Leu Ala Ile Arg Ser Tyr Pro Ala Ala Gly Lys 3505 3510 3515 3520 Thr Phe Leu Asp Tyr Leu Lys Glu Ile Lys Glu Thr Thr Leu Gly Ala 3525 3530 3535 Phe Glu His Gln Asn Tyr Pro Phe Glu Glu Leu Val Asp Lys Val Asn 3540 3545 3550 Val Ala Arg Asp Leu Ser Arg Asn Pro Leu Phe Asp Thr Met Phe Ala 3555 3560 3565 Leu Gln Asn Thr Glu Asn Leu Glu Ile Gln Leu Pro Gly Leu His Leu 3570 3575 3580 Ser Thr Tyr Ala Ser Glu Glu Ile Val Ser Lys Phe Asp Leu Ser Leu 3585 3590 3595 3600 Asp Val Thr Glu Ile Glu Glu Gly Leu Glu Tyr Leu Phe Glu Tyr Ala 3605 3610 3615 Thr Ala Leu Tyr Lys Thr Glu Thr Val Glu Lys Leu Ala Ala His Tyr 3620 3625 3630 Leu Gln Leu Leu Glu Ser Ile Leu Arg Asn Pro Ser Ala Thr Ile Ala 3635 3640 3645 Glu Leu Gly Ile Leu Thr Pro Ala Glu Lys Glu Gln Ile Leu Gly Ala 3650 3655 3660 Phe Asn Pro Ala Gln Pro Glu Ala Ala Pro Ala Ala Ala Phe His Arg 3665 3670 3675 3680 Leu Phe Glu Glu Gln Ala Glu Arg Thr Pro Glu Ala Ala Ala Val Val 3685 3690 3695 Tyr Glu Ser Asp Arg Leu Thr Tyr Ala Glu Leu Asn Glu Arg Ala Asn 3700 3705 3710 Arg Leu Ala Ala Thr Val Arg Ala Ser Gly Ile Gly Arg Asp Thr Ile 3715 3720 3725 Val Gly Ile Leu Ala Glu Arg Ser Val Asp Leu Leu Val Ala Val Leu 3730 3735 3740 Ala Val Trp Lys Ala Gly Gly Ala Tyr Val Pro Leu Asp Pro Asp Tyr 3745 3750 3755 3760 Pro Ala Glu Arg Val Arg Phe Met Leu Glu Asp Ser Gly Ala Lys Val 3765 3770 3775 Leu Leu Thr Gln Thr Leu Leu Arg Glu Arg Ala Glu Ala Trp Leu Gly 3780 3785 3790 Glu Glu Glu Leu Ala Leu Ala Ala Val Leu Tyr Leu Asp Asp Glu Ala 3795 3800 3805 Ser Tyr Ser Glu Glu Arg Ala Asn Ala Pro Leu Glu Ala Asp Asp His 3810 3815 3820 Leu His Glu Cys Arg Pro Glu Asp Leu Ala Tyr Val Ile Tyr Thr Ser 3825 3830 3835 3840 Gly Thr Thr Gly Lys Pro Lys Gly Val Met Ile Glu His Arg Ser Leu 3845 3850 3855 Val Asn Thr Ala Ala Gly Tyr Arg Arg Glu Tyr Gln Leu Asn Gln Phe 3860 3865 3870 Pro Val Arg Leu Leu Gln Leu Ala Ser Phe Ser Phe Asp Val Phe Val 3875 3880 3885 Gly Asp Ile Ala Arg Thr Leu Tyr Asn Gly Gly Thr Met Val Ile Val 3890 3895 3900 Pro Lys Asp Asp Arg Ile Asn Pro Ser Arg Leu His Tyr Trp Ile Ser 3905 3910 3915 3920 Gln Gln Gln Val Thr Ile Phe Glu Ser Thr Pro Ala Leu Ile Val Pro 3925 3930 3935 Phe Leu Glu Tyr Val His Glu His Gln Leu Asp Met Ser Gly Met Glu 3940 3945 3950 Leu Leu Ile Thr Ser Ser Asp Ser Cys Ser Val Ala Asp Tyr Arg Thr 3955 3960 3965 Leu Gln Glu Arg Phe Gly Ser Leu Phe Arg Ile Ile Asn Ala Tyr Gly 3970 3975 3980 Val Thr Glu Ala Ala Ile Asp Ser Ser Phe Tyr Asp Glu Glu Leu Ala 3985 3990 3995 4000 Lys Leu Pro Gln Thr Gly Asn Val Pro Ile Gly Lys Ala Trp Leu Asn 4005 4010 4015 Ala Lys Phe Tyr Ile Val Asp Ala His Leu Asn Pro Val Pro Val Gly 4020 4025 4030 Val Leu Gly Glu Leu Val Ile Gly Gly Val Gly Val Ala Arg Gly Tyr 4035 4040 4045 Leu Asn Arg Pro Glu Leu Thr Glu Glu Lys Phe Val Asp Ser Pro Phe 4050 4055 4060 Ala Ala Gly Glu Arg Leu Tyr Arg Thr Gly Asp Leu Ala Arg Trp Met 4065 4070 4075 4080 Glu Asp Gly Asn Val Asp Phe Ile Gly Arg Ile Asp Asn Gln Val Lys 4085 4090 4095 Ile Arg Gly Tyr Arg Ile Glu Thr Gly Glu Val Glu Ala Lys Leu Leu 4100 4105 4110 Ser Val Gly Gly Val Lys Glu Ala Val Val Val Val Arg Glu Asp Gln 4115 4120 4125 Glu Gly Gln Lys Ala Leu Cys Ala Tyr Tyr Thr Ala Glu Glu Asp Leu 4130 4135 4140 Thr Ala Ala Asp Leu Lys Arg Ala Ile Ala Ser Glu Leu Pro Gly Tyr 4145 4150 4155 4160 Met Ile Pro Ser Tyr Phe Val Glu Leu Glu Arg Leu Pro Leu Thr Pro 4165 4170 4175 Asn Gly Lys Ile Asp Arg Lys Ala Leu Pro Ala Pro Glu Gly Gly Ala 4180 4185 4190 Gly Gly Gly Arg Glu Tyr Val Ala Pro Arg Thr Glu Leu Glu Ala Lys 4195 4200 4205 Leu Ala Ala Ile Trp Gln Asp Val Leu Val Arg Glu Lys Ala Val Gly 4210 4215 4220 Val Thr Asp Asn Phe Phe Asp Leu Gly Gly His Ser Leu Arg Ala Thr 4225 4230 4235 4240 Thr Leu Val Ser Lys Met His Lys Glu Leu Gly Val Glu Phe Pro Leu 4245 4250 4255 Arg Asp Val Phe Arg Tyr Ser Thr Val Glu Glu Met Ala Thr Ala Met 4260 4265 4270 Glu Arg Leu Glu Ile Gly Ser Phe Thr Ala Ile Pro Ala Ala Glu Pro 4275 4280 4285 Ser Glu Tyr Tyr Pro Leu Ser Ser Ala Gln Lys Arg Leu Tyr Ile Leu 4290 4295 4300 Asn Gln Leu Glu Gly Ala Glu Leu Ser Tyr Asn Ile Pro Gly Ala Met 4305 4310 4315 4320 Leu Leu Glu Gly Glu Leu Asp Arg Gln Arg Phe Glu Glu Ala Phe His 4325 4330 4335 Gly Leu Val Ala Arg His Glu Thr Leu Arg Thr Gly Phe Glu Met Val 4340 4345 4350 Lys Gly Glu Ala Val Gln Arg Ile Tyr Glu Glu Ala Ala Phe Gln Val 4355 4360 4365 Glu Tyr Val Gln Ile Ser Gly Glu Gln Ala Glu Glu Thr Val Arg Gln 4370 4375 4380 Phe Val Arg Pro Phe Asp Leu Ala Lys Pro Pro Leu Leu Arg Val Gly 4385 4390 4395 4400 Leu Ala Glu Leu Ala Pro Asp Arg His Ile Leu Met Phe Asp Thr His 4405 4410 4415 His Ile Val Ser Asp Gly Val Ser Ile Asp Val Leu Ile Glu Glu Phe 4420 4425 4430 Val Arg Leu Tyr Ser Gly Glu Gln Leu Glu Pro Leu Arg Ile Gln Tyr 4435 4440 4445 Lys Asp Tyr Ala Val Trp Gln Gln Ser Asp Glu Gln Lys Ala Gln Leu 4450 4455 4460 Ala Lys Gln Glu Ala Tyr Trp Leu Asp Met Phe Arg Gly Glu Leu Pro 4465 4470 4475 4480 Val Leu Glu Leu Pro Thr Asp Tyr Pro Arg Pro Ala Met Gln Ser Tyr 4485 4490 4495 Glu Gly Arg Thr Leu Gln Leu Phe Met Asp Ser Glu Lys Ser Glu Gly 4500 4505 4510 Leu Lys Arg Leu Ala Ala Glu Asn Gly Ala Thr Leu Tyr Met Val Leu 4515 4520 4525 Leu Ala Gly Tyr Thr Met Leu Leu His Lys Tyr Thr Gly Gln Glu Asp 4530 4535 4540 Val Val Val Gly Thr Pro Ile Ala Gly Arg Asn His Ser Asp Val Gln 4545 4550 4555 4560 Pro Leu Ile Gly Met Phe Val Asn Thr Leu Ala Ile Arg Ser Tyr Pro 4565 4570 4575 Ala Ala Gly Lys Thr Phe Leu Asp Tyr Leu Lys Glu Ile Lys Glu Thr 4580 4585 4590 Thr Leu Gly Ala Phe Glu His Gln Asn Tyr Pro Phe Glu Glu Leu Val 4595 4600 4605 Asp Lys Val Asn Val Ala Arg Asp Leu Ser Arg Asn Pro Leu Phe Asp 4610 4615 4620 Thr Met Phe Ala Leu Gln Asn Thr Glu Asn Leu Glu Ile Gln Leu Pro 4625 4630 4635 4640 Gly Leu His Leu Ser Thr Tyr Ala Ser Glu Glu Ile Val Ser Lys Phe 4645 4650 4655 Asp Leu Ser Leu Asp Val Thr Glu Ile Glu Glu Glu Gly Leu Glu Tyr Leu 4660 4665 4670 Phe Glu Tyr Ala Thr Ala Leu Tyr Lys Thr Glu Thr Val Glu Lys Leu 4675 4680 4685 Ala Ala His Tyr Leu Gln Leu Leu Glu Ser Ile Leu Arg Asn Pro Ser 4690 4695 4700 Ala Thr Ile Ala Glu Leu Gly Ile Leu Thr Pro Ser Glu Lys Glu Gln 4705 4710 4715 4720 Ile Leu Gly Ala Phe Asn Pro Ala Gln Pro Glu Ala Ala Pro Ala Ala 4725 4730 4735 Ala Phe His Arg Leu Phe Glu Glu Gln Ala Glu Arg Thr Pro Glu Ala 4740 4745 4750 Ala Ala Val Val Tyr Glu Ser Asp Arg Leu Thr Tyr Ala Glu Leu Asn 4755 4760 4765 Glu Arg Ala Asn Arg Leu Ala Val Thr Leu Arg Thr Ser Gly Ile Gly 4770 4775 4780 Arg Asp Thr Ile Val Gly Ile Leu Ala Glu Arg Ser Val Asp Leu Leu 4785 4790 4795 4800 Val Ala Val Leu Ala Val Trp Lys Ala Gly Gly Ala Tyr Val Pro Leu 4805 4810 4815 Asp Pro Asp Tyr Pro Ala Glu Arg Val Arg Phe Met Leu Glu Asp Ser 4820 4825 4830 Gly Ala Lys Val Leu Leu Thr Gln Thr Leu Leu Arg Glu Arg Ala Glu 4835 4840 4845 Ala Trp Leu Gly Glu Glu Glu Leu Ala Leu Ala Ala Val Leu Tyr Leu 4850 4855 4860 Asp Asp Glu Ala Ser Tyr Ser Glu Glu Arg Ala Asn Ala Pro Leu Glu 4865 4870 4875 4880 Ala Asp Asp His Leu His Glu Cys Arg Pro Glu Asp Leu Ala Tyr Val 4885 4890 4895 Ile Tyr Thr Ser Gly Thr Thr Gly Lys Pro Lys Gly Val Met Ile Glu 4900 4905 4910 His Arg Ser Leu Val Asn Thr Ala Ala Gly Tyr Arg Arg Glu Tyr Gln 4915 4920 4925 Leu Asn Gln Phe Pro Val Arg Leu Leu Gln Leu Ala Ser Phe Ser Phe 4930 4935 4940 Asp Val Phe Val Gly Asp Ile Ala Arg Thr Leu Tyr Asn Gly Gly Thr 4945 4950 4955 4960 Met Val Ile Val Pro Lys Asp Asp Arg Ile Asp Pro Ser Arg Leu His 4965 4970 4975 His Trp Ile Glu Gln Glu Arg Val Thr Ile Phe Glu Ser Thr Pro Ala 4980 4985 4990 Leu Ile Val Pro Phe Leu Glu Tyr Val His Glu His Arg Leu Asp Met 4995 5000 5005 Ser Trp Met Glu Leu Leu Ile Thr Ser Ser Asp Ser Cys Ser Val Ala 5010 5015 5020 Asp Tyr Arg Thr Leu Gln Glu Arg Phe Gly Ser Leu Phe Arg Ile Ile 5025 5030 5035 5040 Asn Ala Tyr Gly Val Thr Glu Ala Ala Ile Asp Ser Ser Phe Tyr Asp 5045 5050 5055 Glu Glu Leu Ala Lys Leu Pro Gln Thr Gly Asn Val Pro Ile Gly Lys 5060 5065 5070 Ala Trp Leu Asn Ala Lys Phe Tyr Ile Val Asp Ala His Leu Asn Pro 5075 5080 5085 Val Pro Val Gly Val Leu Gly Glu Leu Val Ile Gly Gly Val Gly Val 5090 5095 5100 Ala Arg Gly Tyr Leu Asn Arg Pro Glu Leu Thr Glu Glu Lys Phe Val 5105 5110 5115 5120 Asp Ser Pro Phe Ala Ala Gly Glu Arg Leu Tyr Arg Thr Gly Asp Leu 5125 5130 5135 Ala Arg Trp Met Glu Asp Gly Asn Val Asp Phe Ile Gly Arg Ile Asp 5140 5145 5150 Asn Gln Ala Lys Ile Arg Gly Tyr Arg Ile Glu Thr Gly Glu Ile Glu 5155 5160 5165 Ser Gln Leu Leu Arg Val Glu Gly Val Arg Glu Ala Val Val Leu Val 5170 5175 5180 Arg Ser Asp Ala Asn Gly Gln Lys Ala Leu Cys Ala Tyr Tyr Thr Thr 5185 5190 5195 5200 Asp Gly Glu Leu Thr Val Ala Asp Leu Lys Arg Ala Ile Ser Ser Glu 5205 5210 5215 Leu Pro Gly Tyr Met Ile Pro Ser Tyr Phe Val Glu Leu Glu Arg Leu 5220 5225 5230 Pro Leu Thr Pro Asn Gly Lys Ile Asp Arg Lys Ala Leu Pro Ala Pro 5235 5240 5245 Glu Gly Gly Ala Ser Ala Gly Arg Glu Tyr Met Ala Pro Arg Thr Glu 5250 5255 5260 Leu Glu Ala Lys Leu Val Ala Ile Trp Gln Asp Val Leu Gly Pro Val 5265 5270 5275 5280 Thr Ile Gly Val Thr Asp Asn Phe Phe Asp Leu Gly Gly His Ser Leu 5285 5290 5295 Arg Ala Thr Thr Leu Val Ser Lys Val His Lys Glu Leu Ser Val Asp 5300 5305 5310 Leu Pro Leu Arg Glu Val Phe Arg His Ser Thr Ile Glu Ala Met Ala 5315 5320 5325 Glu Ala Ile Ser Gln Leu Glu Arg Gln Glu His Leu Ser Ile Pro Val 5330 5335 5340 Leu Asp Lys Arg Asp Tyr Tyr Pro Leu Ser Ser Val Gln Lys Arg Leu 5345 5350 5355 5360 Tyr Ile Gln Gln Gln Met Glu Gly Ala Glu Leu Ser Tyr Asn Met Ser 5365 5370 5375 Gly Met Thr Val Leu Val Gly Arg Leu Glu Arg Asn Gln Phe Glu Ala 5380 5385 5390 Ala Leu Lys Gly Leu Ile Ala Arg His Glu Ile Leu Arg Thr Gly Phe 5395 5400 5405 Glu Met Val Asp Gly Glu Pro Val Gln Arg Ile Tyr Pro Asn Leu Lys 5410 5415 5420 Phe Ala Val Glu Tyr Thr Lys Ala Thr Glu Ser Glu Thr Lys Ser Ile 5425 5430 5435 5440 Val Asp Gly Phe Val Arg Val Phe Asp Leu Glu Arg Pro Leu Leu 5445 5450 5455 Arg Val Gly Leu Val Glu Trp Glu Ala Glu Arg His Leu Leu Met Leu 5460 5465 5470 Asp Ile His His Ile Val Thr Asp Gly Met Ser Met Gly Ile Phe Val 5475 5480 5485 Glu Glu Leu Leu Arg Leu Tyr Asn Gly Glu Thr Leu Glu Pro Leu Arg 5490 5495 5500 Ile Gln Tyr Lys Glu Phe Ala Ala Trp Gln Gln Ser Glu Pro Val Lys 5505 5510 5515 5520 Glu Arg Leu Lys Arg Gln Glu Ala Tyr Trp Leu Asp Val Leu Glu Gly 5525 5530 5535 Glu Leu Pro Thr Leu Glu Leu Pro Thr Asp Phe Val Arg Pro Ala Ala 5540 5545 5550 Arg Ser Phe Glu Gly Asp Val Leu Pro Phe Ser Ile Asp Lys Gln Met 5555 5560 5565 Thr Asp Ser Leu Gln Arg Ile Ala Asp Lys Asn Gly Ala Thr Leu Tyr 5570 5575 5580 Met Val Leu Leu Ala Ala Tyr Ser Ile Leu Leu Ser Lys Tyr Ser Gly 5585 5590 5595 5600 Gln Glu Asp Phe Ile Val Gly Met Pro Val Ser Gly Arg Thr His Ala 5605 5610 5615 Asp Leu Glu Pro Leu Ile Gly Met Phe Val Asn Thr Leu Ala Ile Arg 5620 5625 5630 His Tyr Pro Ser Gly Glu Lys Ala Phe Leu Ala Tyr Leu Asn Glu Val 5635 5640 5645 Lys Glu Thr Met Leu Gly Ala Tyr Asp His Gln Asp Tyr Pro Phe Glu 5650 5655 5660 Glu Leu Val Lys Lys Leu Gln Ala Pro Arg Asp Gln Ser Arg Asn Pro 5665 5670 5675 5680 Val Phe Asp Val Met Phe Ala Leu Glu Thr Lys Glu Asp Asn Val Gln 5685 5690 5695 Ser Phe Gly Asp Ile Lys Ile Glu Ser Tyr Pro Glu Thr His Thr Val 5700 5705 5710 Ser Gln Phe Asp Leu Thr Leu Val Ile Ser Leu Leu Asp Glu Gly Met 5715 5720 5725 Asn Gly Gln Phe Glu Tyr Ala Thr Lys Leu Phe Thr Arg Asn Leu Ile 5730 5735 5740 Asp Asn Phe Ala Gln Asp Leu Leu Met Ile Ile Thr Gln Ile Cys Glu 5745 5750 5755 5760 Gln Pro Ser Val Leu Leu Lys Asp Ile Ser Leu Asn Gly Gln Ser Glu 5765 5770 5775 Gln Glu Gln Asp Val Leu Glu Ala Ile Asp Ile Ile Phe 5780 5785 <210> 7 <211> 14880 <212> DNA <213> Paenibacillus polymyxa F4 <400> 7 atggcttttg aaaaagaaac gttgttttgg aacgaaaaat tcggtagcga cgattatacc 60 ttgacgcggc tgccttacag caaagctcca agctccctgg cgcccattat gacaaccgtc 120 ggcgggacgc tttcggagga agtggcgcag cgcgtccttc aaatgagcaa gggcgctccg 180 ctggccactt ttatgatttt gctcgctggt gttcagtcgc tcttgcataa atatacaggc 240 gcttctgaca ttctggtcgg catgccggtt gtacggaaac cgacggagac gcgccgatcc 300 gttaatcata cgatcatttt gaaaaactcg ctttcggcgg gtgcgacttt taaaacgctt 360 ctgagcgagc tgaggacttc cttgccggaa gcgattcagc atcaacatat tccgttcttg 420 aaaatgacgg agaagctgga cctgcaatat gcaggcggga tacccgtcgt ccatacgcta 480 gtatccctta aggagctgca tctggacgaa atcgggcaaa acgtggtcac agattgttcc 540 tttgaattca gcttaaccgg cgggacgata cagctagcgc tgtcatataa cgagcactta 600 tatgactccg agttcatgac tcgggtcgtc ggccatctga atcgcctgct ggccgtgggg 660 cttcacgagt tggagctgga catcgtgcgg gcggacatgc tgtcggagga cgagaaattt 720 caattgctgc aaagctttaa cgataccgag aaggactatc cccgtgatcg gacgattcat 780 cagctcgtgg aggagcaggc gaagcgggtg cccgaagcga cggcggttgt ctttgagggg 840 cggcggcttt cgtacgcgga gctgaacgaa cgggcgaacc ggctggcgcg gacgctgcga 900 tcggtcggcg tgctgcccaa tcagctggta ggcatgatgg ccaggagatc gctggagacg 960 gtcgttggca ttctagcggt tttgaaagct ggcggtgcct acgtgccgat cgacccggaa 1020 tacccggaag aacgcatccg ctacattctg gagaactcga acgcgcagct gctgctgact 1080 caaagggagt tgctgcagca ggtgccgttc gaagggaccg tggtggcgct ggatgacgag 1140 caggcctaca gcgacgatgg ctcgaacctg gagccggcca gcggtccgaa cgatctggct 1200 tatgtcatct atacatcagg tacgacgggc aaacccaaag gggtcatgct ggagcatcgc 1260 gggctggtca gcctgaaact gacgtttgcc catacgcttc acacgaccga acaggatcga 1320 gtgctgcaat tcgccagcct atcgttcgat gcgtcgtgct gggagatgtt caacgctctg 1380 tattttgggg cgaccttata tataccgtca accgagacga ttttggatga tcagctgttc 1440 gaacgtttca tgaacgagca tgcaattacg atcgccactt tgccgccaac ttatgcggct 1500 tatttgaatt cagaccgcct gccgagtctg agcaggctca tcacggcggg ctccgccgtt 1560 tcagccgaat tcgtgcagca gtggaaagac aaggtacagt actataatgc gtacggtccg 1620 acggaagcct cgatcgccac atccgtgtgg gcggcgtcta cgcatgacac cgaacgcaga 1680 gcgataccga tcggacgtcc gatctggaac catcgcttgt atatactggg cgcacagaat 1740 cagctggcgc ctatcggagt ggagggagaa ttatgcatcg cgggcgtcgg cctcgcccgg 1800 ggatattgga accggccgga tctgacggcg gagaaattca tcgaacaccc gtttgagccc 1860 ggagagagac tgtaccggac gggcgacttg gcccgctggc tgccggacgg aaatattgag 1920 tacttgggcc ggattgacca tcaggtgaaa atccgtggat tccggatcga gatcggcgag 1980 attgaagagc agcttctgaa gatcgactcc gtgcaggaga cgatcgtaat cgcgcgggaa 2040 ggcaaaagcg ggcaagaact gtgcgcttac ctggtcgcgg ggcgcccgct tacgctcggc 2100 gagctaagaa gcgcgctggc gcaaaaattg ccgaattaca tgattccggc gcattttgtt 2160 cagcttccgc agatgccgct cacgccgaac gacaaaatcg accgcaaggc tttgcccgct 2220 ccggaaggaa acgcgctgac cggcggcgcg tacgtagctc cccgcaatga agccgagcgg 2280 acgcttgccg atgtgtggca ggcggtattg aacgccgatc gcgttggggt aacggatcat 2340 ttcttcgagc tgggcggaga ctcgatcaag tccattcaag tatcttcgcg gcttcatcaa 2400 gccgggtaca agctggaaat ccgggatttg ttcaaatatc cgactatttc acagctcagc 2460 ctgcatgtga aaccgatcgg acgtaccatt gatcaaggcg aaataacggg tgaaacggcg 2520 ctgatgccga ttcagcattg gtttttcgag agctcctttg cggacccgca tcatttcaac 2580 cagtcggtga tgctgtaccg gaaggaacgc ttcgacgaag agacggtgcg tcaggtgctg 2640 caaaagctgg ccgagcatca tgacgccttg cggatggtgt tccgcaagac ggaacaaggg 2700 tttagcgcgc ggaaccgcgc gattcaggaa ggcgggctgt tcacgctgga cgtgttcgac 2760 ttcaaggatg cggagaatac cgcgcaggct gtggaagcga aggcaacgga cattcaagcg 2820 ggcatcgatc tggagaacgg gcccctcgcg aaggcgggac tgttccggtg cgcggacggc 2880 gatcatttgc tgctcgcggt tcatcatgcc gtggtggacg gcgtgtcttg gcgcattttg 2940 atggaggatt tcgctctggg ttacgagcag gccggcaaaa gcgaggaaat tcgtttcccg 3000 gcgaaaacgg atgcgtaccg cacttggtcc gagcagttgg ccgcttacgc gcaaagcccg 3060 gagatggcga aggaacgggc ctattggcag gccgtggaac aaattgcggt tccggccgtg 3120 ccgaaggatc tggaggcgga cgttacgacg cagcaggaca gcgaatcgct gttcgtccgt 3180 ttgactcccg aagaaacgga gctgctgctg aagcgggttc accgggccta caacaccgaa 3240 atgaacgata ttttggtaac ggcgctcggc atagccgttc gcaagtggac gggacacgaa 3300 cgggtgcgga tcaatctcga aggacacgga cgcgaatcga tcggaacgga tatcgacatc 3360 acgcgcacag tcggctggtt tacgaccaag tttccggtcg tcctggagcc ggaaaccgac 3420 cgggatttgg cctatcagat taaacaggtc aaggaaagct tgcgccgcat tccgaacaag 3480 gggcttgggt acggcgtatg ccgctatctc tccaaatcgg aggatggctt tgtttggggc 3540 gcagagccgg aaattaattt taactacctc ggccagttcg gcgatgatgt caatcaggac 3600 gagatcggca tatcttctta ttccagcggc agcccggcca gcgaccggca ggcccgcagc 3660 tttgtgctgg atatcaacgg catggtgctg gacggcgctt tatcgctcga tctcagctac 3720 agccggaagc agtatcgcaa ggaaacgatg gaagccttcg ctcagcggct tgagcaaagt 3780 ctccgagagc tcattaccca ctgcgcaggc aaagaaaaca ccgaattgac gccgagcgac 3840 gtgcaattta aaggcttgac catcgcggaa ttggagcaaa tcgcccagcg ctcgggccat 3900 ctcggggaaa tcgaaaatat ttactcgctt acgccgatgc agaagggcat gtggttccac 3960 agcgcgcttg accggcaaac ggccgcttac ttcgagcaga cgcggtttac gatgcgggga 4020 gcgctcgacg tccagctttt cgagaggagc tggatggagc ttgcgaaacg tcatctggtg 4080 ctgcgggcga attttgtgaa aggaccggcg ggcgagccgc tgcaaatcat ataccgcgac 4140 aagccggtcg gctttgaata tgaagagctg cttcatttgc aggcggacga gaaacaagcg 4200 tatttggata aaaaggcgga ggatgacaag cttcgcggct tcgacatgga acatgacgcg 4260 ctcgttcggg ttacgatcct acgcaccgaa gagcaaagct atcatgtgct gtggagtttc 4320 cagcatattt tgatggacgg ctggtgcctg ccgcagctga cgcaggagct gtttgagacg 4380 tactcggcct tggcatccgg caagcagcca gcgggagata agggatcgga ttatggcgct 4440 tatatcgaat ggttggagaa acaggacgat caggcggcat ccggctattg gacggcattc 4500 ctggcaggtt atgaagggca aaccgtactc ccgggacaaa aggaaccggc gccaaacggc 4560 agatttacgg ctgatcacgt caccgccgag ctgggcaagg acttgagcga gcggatggac 4620 cgggtggcga aacagcgcct ggttacggtc aatacgctgc tgcaagccgc ttggggcgtg 4680 atgctgcaaa aatataacgg aacaaacgat gccgtattcg gcagcgtcgt ggccggaaga 4740 ccggcggaaa tcccgggcat cgagtccatg atcgggctgt tcatcaatac ggtgccggtc 4800 cgcgtcacga gcgaagcgga caccgtgttc gccgacctga tggcgaagct ccaagagcgg 4860 gcgctggagt ccgggcgtta tgattactat ccgctgtatg aaattcaagc ccgcagcgtg 4920 caaaagcaga acctgatcaa ccatatcatc gctttcgaga actatccggt ggacgagcag 4980 atggagcagg cgggcgacca gcagcacggc gacctgacga tcgctgacgt tcagatggag 5040 gagcagacga actataactt caacgtgacc gtggtgccgg gagtcgagat cgaaattcga 5100 ttcgacttta acgccgaagt gttcgataaa gacagcatcg agcggctcaa ggggcatctc 5160 gtccatctgc tggagcaggt gacggataac ccggaaatta ccgtgggcga gctggaactt 5220 gtgacggagg cggaaaaggc cgaccttctc ggacgtttta acgacaccac cacggaattt 5280 ccgcgcggga agacgctcat tcaattgttc gaagagcagg cggagcgcat cccggatgca 5340 gccgctatct ccttgaatga gcaagagctg acctaccgcg agctgaacga acgcgtcaac 5400 cgccttgccc gtaccttgcg tagccacggg atatccaaag gccgtctggt cgccattatg 5460 gctgagcgtt ccatcgaaat ggtggtgggc atgctggcgg cacacaaagc cggagcggct 5520 tacgtaccga ttgacccgga atatcccgag gagcgtatcc gtttcttgat tgaggattcg 5580 ggagcgcagg tcatgctgac gcaaagccgc ttgcgcgagc gcctggcggg ttcggactcc 5640 gtgatcttac tggatgacga gtccttctat cacgaggacg gcacaaatct aaatccgggc 5700 accgaagcga cggatctggc ctgcgtcatc tatacgtcag gcacgacggg caagccgaaa 5760 ggcaaccttg tttcgcaccg caacatcgtg cggatcgtgc gaaatacgaa ttatatcgac 5820 atcaccgaac gggatcatgt cctccagctt tcgagctatt cgttcgacgg agcgattttc 5880 gatattttcg gcgctttgac caacggggcg cggctggtgc tggttccccg cgagactttg 5940 ctggaaatcg tccggctggc ggatctcatc cagcgtgagc gcatctcggt catgctgatt 6000 acgacggctt tcttcaacgt ccttgtagat gtgaacgtcg actgcctgcg ggatgtccgg 6060 gcgattttgt tcggaggaga gcgagtgtcg gtcggacatg tgcgtaaagc gctcgcccat 6120 atcggaccgg gcaggctcaa ccacctgtac ggcccgtcgg aaagcacggt ttataccacg 6180 taccttccgg tcgacttcgt cgatgagtcg gcggttactg tacccatcgg acggccgatc 6240 agtaatacga aggtgtatat cgtcgacagc cggaataagc ttctgccgat cggcgtggcc 6300 ggggagcttt gcgtcggcgg agaaggcttg gtacggggct acaataaccg gccggagctg 6360 acggcggaga aatttgtgga caatccgttt gtgctgggag agcgcatgta ccggacgggg 6420 gatttggcga aatggctgcc ggacggcacg atcgaatacg tgggacggac ggacgaccaa 6480 gtgaaaatcc gcggcttccg tattgagctg ggcgagatcg aagctcagct tcagaaagtg 6540 gagggaattc ggaaagcgac ggtattcgcg agggaagacg cctccggcga gaagcagctt 6600 tgcgcttatt atgaagcgga ccgcgagctt ccggcggccg agctgaagag cgtgctttcc 6660 caggaactgc cggcctatat gattccggcg tacctgatcc agttggagca gctcccgctg 6720 acgacgaacg gcaaggtcga ccgccgatca ctcccggcgc cggaggagag cttgcagccg 6780 ggcgaagaac gtactccgcc tcggactccg ctggaagcca gcttggcggg aatttggaaa 6840 agcgtgctcg gactggagca catcggggtt catgacaact tcttcgacct gggcggccat 6900 tccctgcggg cgacgacact ggtgagcaag gtgcatcagg agctgaacgt cgagctgcct 6960 ctgcgcgacg tattccgcta ctcgacgatc gaagagatgg ctctcgccat ctcccggatc 7020 ggagagcagt cgttctcgtc gattccgctg gcgggcgcaa gagcatatta tccgctttcc 7080 tcagctcaga agcggctgtt tatcctgaat cagctggaag gggccgatca gagctacaac 7140 atgccgggcg tgctgctgct ggaaggatcg attgaccgga gcctgctgga gaaggctttc 7200 cgcggactga tcgcacggca cgaaacgctg cgaaccggct ttgagatcgt acaaggcgaa 7260 gctgtacagc gcatttacga gagcgtcgac tttgccgtcg ggtaccgtca tgtgagcgag 7320 gaagaagcgc ccgaagtcgt gcaggccttc atccggcctt tcgacttggc gaagcctccg 7380 ctgctgcggg cggagctcgt agagctggca gccgaacgtt atttgctgat gttcgacatg 7440 caccatatcg tctccgacgg ggtttcgatg gacgtgttag tcgaggaact cgttcgtctg 7500 tacggcggcg agtcattaga gcctttgcgc attcaataca aggactatgc ggtatggcag 7560 cagtcggacg agcaaaaagt gcagttgaaa cgcgaggaag cttactggct ggaccgttac 7620 cggggcgagc tgccggttct ggaaatgccg acggactatc cgcgtcctgc cgtgcagagc 7680 tttgagggac aaacgctgac gtccttcgtg gacgaggcaa cgaacgaagg cttgaagcag 7740 ctggccgctc aaagaggaac gacgctgtat atggtgctgc ttgcggcata taccgtgctt 7800 ttgcataaat acacaggtca ggacgatttg atcgtcggaa cgtcgattgc gggtagaacg 7860 cacggagaaa cgcagccttt gatcggaatg ttcgtcaata cgctggcgct ccgcaattat 7920 ccggcttcgg agaagacctt cctgtcgtat ctggaagaag tgaaagaaac gaccttaggc 7980 gcttacgagc atcagaatta tccgttcgaa gagctcgtcg ataaagtgca ggtcagccgg 8040 gatttgagcc gcaacccgct gtttgacacg atgttctccc tgcaaaactt ggaggataaa 8100 gagtttgagc tggaagggct gaaattgtcc ccgtacccta gcgaatacgg cacggccaag 8160 ttcgacctga gtgtggatgt tacggaagaa aacggcggcc tggagtgcag ctttgaattc 8220 gcaacggctc tttataaaga aagcacgatc cggcggctgt cgactcattt cggacatttg 8280 ctcgtaacga tcgtaagccg tccggatgcg aagatcgccg agctgaactt gctgacggca 8340 gaggaaaaag aacaaattct cggcgcgttc aacccggcgc agccggaagc ggctcctgcg 8400 tcggcgttcc accggctgtt cgaggaacag gcggagcgca cgccggaagc ggaggccgtc 8460 gtgtacgaga acgaccggct tacgtatgcg gagctgaacg agcgggcgaa ccgcttggcg 8520 gctacgctgc gtgcaagcgg catcggccgg gaggcgatcg tcggcattct cgccgagcgt 8580 tcggtggact tgctggtggc cgtgctggcc gtctggaaag tgggcggggc gtatgtgccg 8640 ctcgacccgg attacccggc ggagcgcgtg cggttcatgc tcgaagacag cggagcgaag 8700 gtgctgctga cgcaaacgcc gctgcgagag cgtgccgaag catggctcgg cgaagaggag 8760 ctggcgctgg cggcggtgct gtacctcgac gacgaagcgt cgtacagcga cgagcgggcg 8820 aatgcgtcgg ttgtctccgg caagctgacg gatgctgtag atgacggcga tgagagccat 8880 cagaatgttg gcaccgacag cttccatgaa gcccgtccgg aggatctggc gtacgtgatc 8940 tatacgtcgg gaacgacggg caagccgaag ggcgtgatga tcgagcaccg cagcctggtg 9000 aatacggcgg cgggctaccg gcgggaatac cggttggatc agttcgcggt gcggctgctg 9060 cagctcgcca gcttctcgtt cgacgtgttc gtgggagata tcgcccggac gctgtacaac 9120 ggaggcacga tggtgattgt gccgaaggac gaccggatcg atccgtctcg tctgcaccac 9180 tggattgagc gggagcgggt caccatcttc gaatcgacgc cggcgctgat cgtgccgttc 9240 ctagagtacg tgtacgagca gcaactggat atgagccggt tggagctgtt gatcacgagc 9300 tcggacagct gcagcgtggc ggattaccgg accttgcagg aacgcttcgg ctcgttgttc 9360 cggatcatca acgcatacgg cgtgacggaa gcggcgatcg actcaagctt ctacgacgag 9420 gagctggcga agctgccgca gacaggcaat gtgccgatcg gcaaagcgtg gctgaatgcg 9480 aaattctaca tcgtggatgc gcatctgaac ccggtgccgg tcggggtgct gggcgagctg 9540 gtcatcggcg gagtcggagt ggcgcgcggg tacttgaacc gtccggagct gacagaagag 9600 aagttcgtag acagcccgtt cgccgagggc gagcggctgt accgcacggg agacttggcg 9660 cggtggatgg aagacggcaa cgtggacttc atcggccgga tcgacaacca ggcgaaaatc 9720 cggggctacc ggatcgagac gggcgagatc gagtcgcagc tgctgcgggt ggaaggcgtg 9780 cgcgaagcgg tggtgctggt tcgaagtgac gcgaacgggc agaaggcgct atgcgcgtat 9840 tacacgccgg ataccggagc ggaactggcg gtgaacgatc tgcgcggcgc gctggcgcag 9900 gagctgccgg gctacatgat cccgtcgtac ttcgtggagc tggagcgcct gcctctgacg 9960 ccgaacggaa agattgaccg gaaggcgctg ccagcgccgg aaggggaagc gggaagcgga 10020 acggagtacg tcgcaccgcg caatgagctg gaaacgaagc tggcggcgat ttggcaggag 10080 gtgctggggc ttgcgaagga gattggcgtt cacgacaact tcttcgacat cggcggccac 10140 tccctgcggg cgacgacgct ggtcagcaag attcataaag agttgaacgt ggatctgccg 10200 ctgcgcgacg tgttccgcca ttccacgatc gagagcatgg cggccgccat ttcccggctg 10260 gatgagcaaa cattcgttgc cattccggtg gcggatgacc gagaggtgta cccgcaatct 10320 tttgctcaaa aacgtctctt tatcctgaat caactggaag gcgcggagct tagctacaac 10380 atgccggaag cgatgctgct ggagggtgct ttagaccggg caaggttcga agaagcgttc 10440 cgtaagctcg tggcgcggca tgaaacactg cgcaccgggt tcgaaatggt ggatggcgaa 10500 gcatcgcagc ggatttacca ggacgtgaat tttgccgtgg agttctatcg agcggatgag 10560 caagaggccg aagagacggt tcgccgtttc gtccgtccgt ttgacttggc gaagcctccg 10620 ctgctgaggg taggccttgt cgagctggct ccggaacgcc atattctaat gtacgacatg 10680 catcatatta tttccgacgg cgtctcgatg gaaatctttg ttgaagaatt cgtccgcttg 10740 tacggcggcg agcaattgga gcctctgcgc attcagtaca aagactacac cgtttggcag 10800 cattcgcagg agcagaagga acggcttcag cgtcaggagg cgtactggct ggacatgttc 10860 caaggcgagc ttccggtgct ggaaatgccg accgactatc cgcgtccggc cgtgcagagc 10920 tacgaaggcc aaacgctgga gtttttcttt gacgcttcga aaaccgacgg cctgaagcag 10980 ctggcctcgg aaacgggcac gacgctgttt atggtgctgc ttgcggcgta taacgtcctt 11040 ctgcataaat attcaggtca ggaagacgtg atcgtcggta cgccgattgc cggaaggaat 11100 catggagatg tgcagccgtt gatcgggatt ttcttaaaca cgctggctat ccgcagttat 11160 ccggcttcgg agaagacatt cctgtcatac ctgaacgaag tcaaagaaac gaccctccac 11220 gccttcgagc atcaaaacta tccgttcgaa gaattggtgg acaaggtgca agtcacccgt 11280 gatttaagcc gcaatccgct cttcgacacg ctgtttacga tgcagaatac ggagaacgag 11340 gaatttgagc tggaagggct tcgcctgatt ccttatccga gcgcactgga taccgcgaag 11400 tttgatatca gcttggatgt gggcgaggag aacggcggct tggattacag cttcgaatat 11460 gcgacggctc tctacaaaag ggagacgatc gaacggctgg caaaacatta cgagcagctg 11520 ctcgtaacga tcgtaagccg tccggatgcg aagatcgccg agctgaactt gctgacggca 11580 gaggaaaaag aacaaattct cggcgcgttc aacccggcgc agccggaagc ggctcctgcg 11640 gccgcgttcc accggctgtt cgaggaacag gcggagcgca cgccggaagc ggaggccgtc 11700 gtgtacgaga acgaccggct gacgtatgcg gagctgaacg agcgggcgaa ccgcttggcg 11760 gctacgctgc gtgcaagcgg catcggccgg gagacgatcg tcggcattct cgccgagcgt 11820 tcggtggact tgctggtggc cgtgctggcc gtctggaaag cgggcggggc gtatgtgccg 11880 ctcgacccgg attacccggc ggagcgcgtg cggttcatgc tcgaagacag cggagcgaag 11940 gtgctgctga cgcaaacgcc gctgcgagag cgtgccgaag cctggctcgg cgaagaggag 12000 ctggcactgg cggcggtgct gtacctggac gacgaagcgt cgtacagcga ggagcgagcg 12060 aatgcgtcgg ttgtctccgg caagctgacg gatgctgtgg acgacggcga tgagagccat 12120 cagaatgttg gcaccgacag cttccatgaa gcccgtccgg aggatctggc gtacgtgatc 12180 tatacgtcgg gaacgacggg caagccgaag ggcgtgatga tcgagcaccg cagcctggtg 12240 aacacggcgg cgggctaccg gcgggaatac cggttggatc agttcccggt gcggctgctg 12300 cagctcgcaa gcttctcgtt cgacgtgttc gtgggagata tcgcccggac gctgtataac 12360 ggaggcacga tggtgattgt gccgaaggac gaccggatcg atccgtctcg tctgcaccac 12420 tggattgagc gggagcgggt caccatcttc gaatcgacgc cggcgctgat cgtgccgttc 12480 ctggattacg tgcacgagca gcaactggac atgagctgga tggagctgtt gatcacgagc 12540 tcggacagct gcagcgtggc ggattaccgg accttgcagg aacgcttcgg ctcgttgttc 12600 cggatcatca acgcatacgg cgtgacggaa gcggcgatcg actcaagctt ctacgacgag 12660 ccgctgacga agctaccgca gacaggcaat gtgccgatcg gcaaagcgtg gctgaatgcg 12720 aaattctaca tcgtggatgc gcatctgaac ccggtgccgg tcggggtgct gggcgagctg 12780 gtcatcggcg gagtcggagt ggcgcgcggg tacttgaacc gtccggagct gacggaagag 12840 aagttcgtag acagcccgtt cgccgcgggc gaacggctgt accgcacagg agacttggcg 12900 cggtggatgg aggacggcaa cgtggacttc atcggccgga tcgacaacca gacgaaaatc 12960 cgggggtacc ggatcgagac gggcgagatc gagtcgcagc tgctgcgggt ggaaggcgtg 13020 cgcgaagcgg tggtgctggt tcgaagtgac gcgaacgggc agaaggcgct atgcgcgtat 13080 tacacgccgg ataccggagc ggagctggcg gtgaacgatt tgcgcagcgc gctggcgcag 13140 gagctgccgg gctacatgat cccgtcgcac ttcgtggaga cggaacgcct gcctctgacg 13200 ccgaacggaa agattgaccg gaaggcgctg ccagcgccgg aaggggaagc gggaagcgga 13260 acggagtacg tcgcaccgcg caatgagctg gaaacgaagc tggcggcgat ttggcaggag 13320 gtgctggggc ttgcgaagga gattggcgtt cacgacaact tcttcgacat cggcggtcac 13380 tccctgcgag cgacgacgct ggcgggcaag gtatttaagg aattaaacgt caacctgccg 13440 ctgcgcgacg tattccgtca ctcgacgatt gcggcgatgg ccgaggcgat cgcccggatg 13500 gaacggcggg agcatgaggc cattcctcaa gcggaggaga gagagtacta ccctctgtcc 13560 tccgcgcaga aacggctgtt cattcagcac acgctggatg gagcggatca gctttacaac 13620 atgccggaac tggtgcaggt ggaaggcgag tttgatttag accggttgga agccgccttg 13680 cggaaattga taacacggca tgaatcgctg cgcaccggtt ttgaaatcgt gaaggccgaa 13740 gcggttcagc ggatttaccc gcaggtcgat tttgctatcg agcatcatca agcggataaa 13800 gaggatgcgg ctcaaatcga gcagatcgtc cgcagcttcg ttcgtccgtt tgatctcggc 13860 aagccgccgc tgctgcgcgc cggggtcatc gagctggagc cgaacctgca tattctcctt 13920 ttcgacatgc accatatggt gtccgacggc gtatcgatgg cgattgtgat cgatgagttc 13980 tcgagtttct acgccgggga agaactgccg ccactgcgca ttcaatacaa ggattatgcc 14040 gtttggcagc agtcgaaggc ccaccgagag cggatcgggc ggcaggaagc gtactggctg 14100 caaaccttcg aaggcgagct gccgacggcg gacctgccga tggactacga acggtccgcg 14160 gctcgcagct acgaaggcgc gcatctggag ttcgacgtcg aagcttctct ctctgcgcgg 14220 ctgcgcgaat tggcggccga gcgtgaaagc acgctgttca tggtgctgct tgcggcttat 14280 accgtgctgc tgtccaagta cagcgggcag gaggacttgg tcgtgggcac cccggtggcg 14340 ggaagaacga acgccgattt ggaaccggtc atcgggatgt ttgtcaatac gctggcgatc 14400 cgcaatcgtc cgtcgggcga caaaacgttc ttgtcctacc tggaagaagt gaaggaaacg 14460 gctttgggtg ctttcgagaa ccaggattat ccattcgagg agctcgtgga gcgtttgaat 14520 gtgaagcggg agccgggccg cttcccgctg ttcgatgccg ttttcgactt gcaaaatatc 14580 gaagaacgag acgccgagct ggaaggggtc agcctgaaga cttacgagct tgaccatttg 14640 gaagaagcga agttcgatct gacgctgttt atgtatgaaa acaacggggc gctgagcggg 14700 ggcttcttct acgccaccaa gcttttcaaa gaagcgatga tccgcacctt gaccgaggat 14760 tacctgcggg tactgtctca aattgcggaa aatccacaac tcgagctaag ccggattgaa 14820 tgtcataaac cggcggcagg cgcaaagagt gccgtcgata cgatcgaatt cgcgttctaa 14880 14880 <210> 8 <211> 3309 <212> DNA <213> Paenibacillus polymyxa F4 <400> 8 atgaaatctt tgtttgaaaa ggaagaacgg tactggagcg gcaagtttga cgccgatgac 60 agcctgagct tccttcccta cagtcaatcc tccaaattat ccgccgacgg ggaagctgcg 120 gccgagccgg gcttgcttca ccgtactctg ccgagcgaac tctcggagag aatcatttgc 180 ctcgccaacg gttcggattt ggctttgtac atgattgttt tggcaggagt aaaaagcctg 240 ctgttcaagt ataccgggcg ggaccaagtg ctggtcggca tgccttctta tagcgcagac 300 cccgacggga ctccgccgcc gcatgacatc ttggtgatca agacgtccgt aagccgccag 360 actacgctga aaacgctgct cgggggcatc aaagcctcca tcggcgaggc gctggagcat 420 cagcacctgc cttttcggaa aatggtggag ccgctccatc tggactatac gggggacggc 480 ctcccggtcg tcaacaccgt cgtatccttc gccccgattc atccggaacc gctgggtaac 540 cgggtggcgg ccgatacggt ttttcgcttc gaccgccaaa accactccat cgagctggaa 600 ataagctttg acgggcagcg ttacgagcgg gcatttgtgg aacaggcggc cgaccatctt 660 gttaggctgc tgtccgtgct tttatttcag ccggatctgg agcttggaca agccgatgtg 720 ctgtccccag acgagaggga gacgctgctg aagcgattta atgacaccga aaccgagttc 780 gagcggggga aaacgattta cggcttgttc gaagaacagg cggagcttta cccggacaac 840 gtggccgccg tcatgaacga gcggcagctg acctaccgcg agctgaacga gcgatccaac 900 cgccttgcgc ggaagctgcg agagacggga gtagaagcgg accagctggt agcgattctg 960 gccgaacgct cgctcgatat ggttgtcggc attctggcga ttctcaaagc gggcggagca 1020 tacgtgcctg tcgatcccga ctacccggag gagcgcatcc gcttcatgat cgaggattcg 1080 ggcgcgccgt tattgctgat tcaaaagcat ctgcacgaga agaccgactt cgcaggaacg 1140 cgccttgaat tggacgattt cgtgtggggc gacagagggg cggactccgc cgatgtgcgg 1200 gacgcttcga acctggagcc gatttccggg ccgggcaacc tggcttatgt catctacacg 1260 tcgggaacga ccggcagacc gaaaggaacg ctgatcgagc ataagaacgt cgtgcgcctc 1320 ctgttcaacg acaagaacct gttcgacttc gggccgtccg acacgtggac gctgttccac 1380 tcgttctgct tcgatttctc cgtctgggaa atgtacggag cgctgctgta cggaggcaag 1440 ctggtcatcg taccgccgct cacggcgaaa aatccggccg atttcctggc gctgctgggc 1500 cgcgaacagg tcacgatttt gaaccagacg ccaacgtact tctaccagct gctgcgtaag 1560 gtcttggcgg accatccgta cgatctgcgg attcgcaacg tcatcttcgg gggcgaagcg 1620 ctgagtccgc tgctgctcaa gggattcaag acgaagtacc cggagacgaa gctgatcaat 1680 atgtacggca ttaccgagac gacggttcac gttacgtata aggaaataac gtgggtcgaa 1740 atggaggcgg cgaagagcaa tatcggcaag ccgatcccga cgctgagcgt gtacgtcctt 1800 gatgaaaacc gctcccctgt gccgatcggt gtagcgggcg aaatgtacgt ggccggggaa 1860 ggccttgcga gaggatacct gaaccgtccg gatctgacgg cggagaagtt cgtcgattcc 1920 ccgtttgcgg agggggagaa actgtaccgc tcgggcgact tggcggcttg gctgccggac 1980 ggcaacatcg aatacctggg ccggatcgat catcaggtga aaatccgtgg gtaccggatc 2040 gagctggacg aaatcgagac gcagctgctg aagatcgccg ccgtgcaaga agccaaggtg 2100 ctcgaccgcg acgacgcgaa cggccataag cagcttgtcg cttattacgt cgcggaaacg 2160 aggctggcgg cgcatgaact caaggaggag ctcgccaagc agcttccggg gtatatgatt 2220 ccttcgcacc tcgtgcagct ttcgcggatg ccgctgaccc cgaacgggaa aatcgaccgc 2280 aaagcgctgc ccgcgccgga ggaagccgcg gccggaggag cggaatatgt cgcgccgaga 2340 acgctgctcg aaatgaagat cgcccgcgtc tggcaggata cgcttggcgt tccgcaggtc 2400 ggcgtaaagg ataacttttt tgagttgggt ggcaattcgt taagtctgat gaggctcgtt 2460 caagccgttt acgatgaaac ggacatcgag ataccgctga accgccaatt ccacaatcta 2520 acagttgaag ccatggcttt cggcgagggg gatctgggcc tggataaagg gggagactcc 2580 ttcattaagc tgaataaagc aggagatctg aacgtgttct gcttccctcc gggcagcggc 2640 ttcggcatcg gttaccgaga gctcgcaagc aggctcgacg gccggttcgt gctctacggc 2700 attgatttta tcgacgatgc cgtcgattac gaggccatgc tgaaccgtta tgtggacgag 2760 atcgtccgca tccagccgga aggaccttac gtgctgctcg gctactgctt cggaggcaac 2820 ctgacgttcg aggtagccaa aacgatggag aaaagagggt attccgtaac ggacgtgctc 2880 atgttggact cgtggattaa ggacacgctg acgccttccg aaacgtcgga gaaagagctt 2940 gaagaaacgc ttgccgattt cgacgaagaa gagaaggaat taatgagcaa cccgctcgtg 3000 cgggagcgag ttcatcggaa ggtcaaagcg accttggcgt acgaagcgca gcttattaat 3060 tccggcacga tcccggccag gatttacgaa ctgattgcga aggacagcga agcgttccgc 3120 ctggagcacc aattgccttc ctggcggggg gcaacgacgc aagcttacgc cgattaccgg 3180 ctggagggcg cgcacgagga attgctggaa ctcgcgcgcg tggaagaaac ggccgtcgtc 3240 atccgggaca tcttagagca agtcaagcgg cggatcgaag cggaggccgg ggtactgcat 3300 ggaagctga 3309 <210> 9 <211> 18849 <212> DNA <213> Paenibacillus polymyxa F4 <400> 9 gtgagagaga ataccaacga gcaatatgga ttaacgcaag cccagcgccg aatatggttc 60 atggaaatta tgaatccggg aacgtccatc acgatgcttt ccgcgaccta ccagattacg 120 ggcgagatca acacacagct tctggagcaa gcggcggcag agatcgtcaa aacctatgac 180 gttttccgaa tccgcattag cggggatttg caaaatccaa cgcagtggtt cgaagaaccg 240 gagaatgtcc aagctaggat aagccgcctc gaaataggca caaccgaaca attctatgct 300 tgggtgaaag aagtaagcga aaaaccggcc agcgtgttcg acgaacacct ctaccaattt 360 acgattatcc attttgcgaa cggccaagta tggctcaatt tgacggtaaa tcatattatc 420 gccgacggct tgtccgtcaa tgctttgctg catgcggtga tggaaaaata cctggaactg 480 cgcaaaggca tctccagcag ttaccaggcc ccttcctatc tggattatat ttctgcggag 540 cgtgaatatg agcaatcgca gcgttatcaa aaaggcaagg aatactggct gacgaagtac 600 aacactttgc ctgaaacgac cggcattaaa tcgtatccgc cattctcgat cggcagcgaa 660 tccaataaac tggccatcac tttggacggt tcccggtatg aacgcattct ggccttcagc 720 gaacaatatc aggtcagctt atatacgtta tttctgtccg ccatgtacgc cttattgtac 780 aagctgaccg acagcaccga tgttccggtc ggcacggttt tcgccaatcg caccagcaag 840 aaggaaaaag aaacgatcgg catgttcgtc agcaccgtgg ctacgcggat ccgtctgaat 900 ccagacaggc acgtgctttc cttgatccaa acggtttcca aggaaaatac ggcggatctg 960 cggtatcaga aataccctta taaccaattg atccaggatt tacgcgaaca acacggccgc 1020 aacgatcttt cggggctgtt ccgcacgtct ctggaatatc tgcctttgaa aatcgtggag 1080 tacgaagaaa tcaaggtacg cctggaggct cacttcgcta ggcacgagat ggacgatttg 1140 ctgctgcgct tcgaccatat gctgaatgaa ggccatgtaa ttctccatgc ttcctatcgt 1200 accggcctgt tcgagacggc cgagattgat cggattatgg aacagtatgt aaccgttctg 1260 gaccagtttc ttcagactcc cgaactgccg gtacgcgaga tttctctgct gagcgatgag 1320 gagagacacc gcattctcaa cgtttttaac ccgccggtgg cagggctgag cgagggagaa 1380 gcgtttcatc ggtatgttga aaagtttgcc ctggaaattc ctgatcatcc ggcagtcgtc 1440 tacatggaca aacagctgac ctacggcgaa ttgaacgaac gcgccgagcg gctggcttct 1500 ctccttcgcg aacagggcgt gggaagggag acgattacgg ggatctgggc ggagcgttcg 1560 gtggaactgc tggtcggggt gctcgccgtt tggaaagccg gcggagccta tgtaccgctg 1620 gaccccgatt atccggcgga gcggattgag tacatgctca gcgatagcga agcaacggtg 1680 ctgcttacgc agcgtcatct gttggagcgg gccggagttt ggttggccga tgaccggctg 1740 aagcttcaag ctgtctatgc catggacgat gaacagattt ataacgggga tgccttagcc 1800 gtggaatttg aatctgccgg cagcgccccg caagacttgg cttatgtgat ttacacctcg 1860 ggtacgacgg gacgcccgaa aggcgtcatg atcgagcacg gtagtctcgt gaatacggcg 1920 gatgcgtacc gtcgcgagta ccggttggat cagtttccgg tgcggctgct gcagctggcc 1980 agcttctcgt ttgacgtgtt cgtcggagac atcgcgcgga cgctgtataa cggaggcacg 2040 atggtgattg tgccgaagga tgaccggatt gatccgaacc tcttatacgg ctggattcgg 2100 gaccaaaata ttacggtatt cgaatcgacg cctgcgctca tcctgccgtt catgcagcat 2160 atttatgaag aagggctgga cgtcagctcc atacagttgc tgattaccag ctcggatgct 2220 tgcagtgtca ccgattaccg attgctgctg gaaagattcg gcggacaatt ccgcatcatt 2280 aacagctatg gcgttaccga agcggccatt gacagcagct tttacggtga gccgctggat 2340 aagctgccgc cgtcgggtca tgtgccgatc ggcaaagctt ggctgaacgc ccggttttac 2400 attgtcgatg ccgcgttaaa gccagttcct gtaggggttc cgggcgagct tgtcataggc 2460 ggcgccgggg tggcgcgcgg gtactggaac cgtctggacc taaccgccga gaagtttgtg 2520 gacagcccgt ttgtgccggg cgaacgtgtg taccggacag gcgatttggc ccgctggttg 2580 gaagacggca acgtcgactt catcggccgg atcgactatc aggtgaaaat tcgcgggttc 2640 cgtatcgaac ttggcgaaat tgaaacggcc ctgctgcgtt tcccgggcgt caagcaggct 2700 gtggtgaccg accgcacgga tgagcagggg caaaagtatt tgtgcggcta cgtggcggcg 2760 gatgcttcct tgcagctgag cgatctgctg tcccaattga agcaagagct gccggcccat 2820 atggtcccgg cccggctggt gtctcttgat aagcttccgc tcacgccgaa cggcaaaatt 2880 gaccgtaaag cgctgcctga accgaccgga gaggtagaaa caggcggtga gtatgtggct 2940 cctcgcacaa cgctggaaac aagacttgct ctcatttggc agcaggtgct gggtattgcg 3000 cgagttggag tccaagacga tttctttgac ttgggtggtc attccttgcg ggcctccacg 3060 ctggtttcca agattcggaa agagctgcaa gtcgaggttc cgctgcggga agttttccgc 3120 tacaccacga tcgaacagct ggcccaaaga atcggcggtt taaggcagca ggagacgtat 3180 gagattacaa aggcggctga ggccgagtac tatccggttt catccgagca aaagcgtctg 3240 tacgtcctgc gccagcttga cggggccgag cgcagctaca atatgtcggc ggcgcttctt 3300 ctcgaaggca agctggaccg cacgcgcgtc gagcacgcgt tccgggcgct gattcagcgc 3360 catgagacgc tgcgtaccgg gatcgagcag gttcaaggcg agcttgtcca gcgcatctat 3420 gacgaggtgg agtttgctgt agattatttc caagcgagcg agtgggaagt ggaccaagtg 3480 gtggaagcat actatcaccc gtttgatctg accaagccgc cacttctccg catcggcctg 3540 atcgaagtcg acgaggatcg ccacattctg ctgttcgata tgcaccatat cgtctcggac 3600 ggcatttcga cagcgctgct cttcgacgag ttcagccgcc tgtatcgggg cgaggagctg 3660 gccccgctgc gcattcaata caaagattat gccgtttggc agcattccga agcttacggg 3720 cagatgctcc agccgcagaa ggagtattgg ctgaaacagc tgtcgggcga gctgccggtc 3780 ttggagctgc cgacggactt cccgcggcct gcggtgcaaa gctttgacgg ccgaaccgtg 3840 aagttttata tcgagaaaga tcggacggag aagctgaaag agctggcggc acggacaggg 3900 acgaccctgt acatggtact gctgtcggct tacaccatcc ttatgcataa atattcgggt 3960 caggaagatc tgatcgtagg aacgccgatt gccggaagaa cgcaggaaga agtgcagccg 4020 atcgtaggga tgtttatcaa cacgctggcc attcgcagcc gtccggagcg ttccaagccg 4080 tacctttcgt acctggaaga aatcaaggac atcacgctcg gggctttcga acaccaaaat 4140 tatttgttcg aagacttggt agaaagtctt cacattccgc gcgcgaccgg ccggaatccg 4200 ctctttgaca cgttcttctc cctacaaaat acggagaacg agcaaattgt catcgagggg 4260 ctggagcaat cgttttatcc actggaaaac caaacatcca agttcgagct gctcctggat 4320 atttcggagc tggacggtca gctcgaatgc cggttggagt acgctacggc tttgtataaa 4380 caagagaccg cggagcggtt cgccagacat tatgacaagc tgctcgaaac catcgcagca 4440 gcgccggacg gggatattgc ctcgctggaa atgctcacgg aggaggaaat ccgcgagttg 4500 gtgcgcggtt tcaacgattc ggaggcggac tacccgcggc agcagacgat tcacggcttg 4560 tttgaagagc aggcggagct ttatccggac aacgtggccg ccgtcatgaa cgagcggcag 4620 ctgacctacc gcgagctgaa cgagcgatcc aaccgccttg cgcggaagct gcgagagacg 4680 ggagtagaag cggaccagct ggtagcgatt ctggccgaac gctcgctcga tatggttgtc 4740 ggcattctgg cgattctcaa agcgggcgga gcatacgtgc ctgtcgatcc cgactacccg 4800 gaggagcgca tccgcttcat gatcgaggat tcgggcgcgc cgttattgct gattcaaaag 4860 catctgcacg agaagaccga cttcgcagga acgcgcctcg aattggacga tttcgttggg 4920 ggcgacagag gggcgaactc cgccgatgtg cgggacgctt cgaacctgga gccgatttcc 4980 gggccgggca acctggctta tgtcatctac acgtcgggaa cgactggcag accgaaagga 5040 acgctgatcg agcataagaa cgtcgtgcgc ctcctgttca acgacaagaa cctgttcgac 5100 ttcgggccgt ccgacacgtg gacgctgttc cactcgttct gcttcgattt ctccgtctgg 5160 gaaatgtacg gagcgctgct gtacggaggc aagctggtca tcgtaccgcc gctcacggcg 5220 aaaagtccag ccgatttcct ggcgctgctg ggccgcgaac aggttaccat tttgaaccag 5280 acaccaacgt acttctacca gctgctgcgt gaggtcttga cggatcatcc atacgatttg 5340 cggattcgca atgtgatctt tgggggcgaa gcgctgagtc cgctgctgct caagggcttc 5400 aagacgaagt acccggagac gaagctgatc aatatgtacg gcattaccga gacgacggtt 5460 cacgttacgt ataaggaaat aacgtgggtc gaaatggagg cggcgaagag caatatcggc 5520 aagccgatcc cgacgctgag cgtgtacgtc cttgatgaaa accgccgccc tgtgccgatc 5580 ggcgtagcgg gcgaaatgta cgtggccggg gaaggccttg cgagaggata cctgaaccgc 5640 ccggatctga cggcggagaa gttcgtcgat tccccgtttg cggaagggga gaaactgtac 5700 cgctcgggcg acttggcggc ttggctgccg gacggcaaca tcgaatacct gggccgaatc 5760 gatcaccagg tcaaaatccg cgggtaccgg atcgagctgg acgaaatcga gacgcagctg 5820 ctaaacgtcg agggcgtgga agaagcggtg gtgcttgccc gtcaggacgg cgggggcgag 5880 aaggcgcttg tcgcctactt tgtggcggac cggacactga cggtcagcga aatgagaacc 5940 tcgctggccc agggaatgcc ggggtacatg atcccgtcgt acttcttgca gcttgagcgc 6000 atgccgctga cgtccaacgg taaagtggac cgcaaagcgc tgccggagcc gcaaggcggc 6060 ttgcaaacgg gcgtcgaata cgtagcgccg cgtaaccgga cggagtccca gcttgtgaag 6120 atctgggagg aagtgctggg ttactccggc attggagtcc tggacaattt cttcgagctc 6180 ggaggccact ccttgcgggc gacgaacctt gtcagcaaga ttcggaagga aatgaacgtc 6240 gaacttccac tgcgcgatgt gttccgctat acgacggtag agtcgatggc cggggctatt 6300 gccagcttgg aagaaacaca gcacagctcg attccgaaag cggaagagaa agcgtactat 6360 ccggtttcct ccgcgcaaaa aaggctgtac gtcctgcacc agctggatag ctcggagctg 6420 aattacaacc tcccaagcgc cttgcaattg gaaggggctt tgaacgaggc caaagtggaa 6480 aaggcgctga ctactttggt ggcccggcac gatatgctgc gcaccggttt tgaaatcgtg 6540 aatggggagc cggttcagcg tattcatccg ctcgtagctt tcaaggtcga gaagcttcaa 6600 gcaagcgaag atcaggttgc ggccattctt gaaggcttca ttcagccttt tgacttgacc 6660 cagccgcctt tgctgcgcgc cctactgatc gaactggaga aagagaaatt cctgctcgcg 6720 ctggatattc atcatattgg ttccgacggc ctttccatgg acgtgctgct gcgcgaattc 6780 gtgcggcttt acaatgggga agaattgccg gagctgcgga ttcaatacaa ggattacgcc 6840 gtttggcagc aatccgagga acagcgccag cgcatcaaac ggcaggagga atactggcgc 6900 ggggtattca gctccgagct tccggttctt gagctgcctc tcgacttctc ccgcccggcc 6960 gtccagcagt ttgacggtca aacgctcacg tttacgctgg atgcggagaa aagcgaagct 7020 ctcaaacggc ttgccggcga ttcgggggcg acgctgtaca tgcttctgct ggctgcttac 7080 tccgtattgc ttcataaata cgcgggacag gaagatatag tggtcggcac tccgattgcc 7140 gcccgttctc acgccgacct gcagccgatt atcggcatgt tcgtcaatac gctggccctt 7200 cgcttgggtc cggtggcgga gcggacgttc ctggattact tgcaggaagt gaaggaaacg 7260 acgcttggag cctacgagca ccaggactat ccgttcgagg agctggtgga agctcttcag 7320 gtgagccggg atttaagccg gaatcctctg tttgacacca tgttttcttt gcaaaagcac 7380 gaaagcttgg atttaaccct ggaaggcttg caatggtcgc tgttcgacat cgaggaaaag 7440 acggcaaagt ttgatcttag ctttgatatc gtggaagccg ataacgagct gatttgcaag 7500 atcgagtacg ctacctcgtt gtttagacag gaaacgatgg tacggctggc gggtcattac 7560 gagcagcttt tggcgtcgat cctggctcag ccgggtgcgc ggatttcgga tttggatatc 7620 ttgacggaca gcgaaaagca tgatttgctg gtcgggtttg acgtgtcgtc ttcggctctt 7680 gcgaagcaac ccgccgcaga gggtacaggt ttggaagcgg atgagtcgtg gagagagaga 7740 acgttccacg agctgttcga ggagcaggcg gagcgcactc cgggagcgct ggcggttgtc 7800 tacgaagaca gcaagctgac gtatgcggag ctgaacgcca aagcgaatcg tctggcgtat 7860 gcactgcggg cgcgcggggt gaagccggag caggtggtcg gcattctggc cggccgttcg 7920 gcggagctgt tgatcggggt gctcgccgta tggaaagcgg gcggcgctta tgtgccgctc 7980 gacccggact atccggcgga gcggatcgag tatatgctcg cggacagcgg ggcgtcggtg 8040 ctgctcacgc agacccgcct gctggagcag gcggaagctt ggcgcagcga cggagctcta 8100 gtgctgcaaa cggtgcttgc gcttgacgac gccgcgacgt acagcctcgg agcggcggaa 8160 gcggctgtgg gcgaagcagg cgcagaggcg gaggctttgg cgcaagcggc tgctgccgaa 8220 tcgtccgcca cggcagaagc cgagaagaac gtactagcgg cggatctcgc atcgaatccg 8280 gcgaatgtga acaagccgcg cgatttggct tacgtcatct acacctccgg tacgacgggc 8340 cgtccgaagg gcgtggcggt ggaaaaccgc agcctggtga acacggcggc gggctaccgg 8400 cgggaatacc ggttggatca gttcccggtg cggctgctgc agctcgcaag cttctcgttc 8460 gacgtgttcg tgggagatat cgcgcggacg ctgtacaacg gcggcaccat ggtcatcgtg 8520 ccgaaggacg accggattga tccaacccgc ctctacggct ggattcgcga ctacgccgtg 8580 acggtgttcg aatcgactcc ggcgctgatc gtgccgttca tggagcatgt gcatgccgag 8640 ggtctggacc tcagctcgat gcagctgctg atcacaagct cggatgcgtg cagcgtagcg 8700 gattaccgca ccttgcagga gcgcttcggc tcgcagttcc gtattattaa cagctacggc 8760 gtaacggaag cggcgattga ctccagcttc tatgacgagc cgctggagaa gctgccgaag 8820 acgggcagcg tgccgatcgg gaaagcgtgg ctgaacgcca agttttacat cgtggatgcg 8880 aatctgaagc cggtgccgat cggggtgttg ggcgagctgg ttatcggcgg agcgggtgtg 8940 gcccgaggtt acttgaaccg cccggatttg acggcggaga aattcgtaga cagcccgttc 9000 gccgctgggg agcggctgta ccggacgggc gacctggcgc gctggatgcc ggacggcaac 9060 gtggacttca tcggccggat cgacaaccag gtgaaaattc gcggctatcg gatcgagctt 9120 ggtgaaattg aagcggccat gaaaaatttt gccggcgtgc gtcaagcgct tgtcatcgac 9180 cggacggacg agcgggggca gaaatatttg tgcgggtatg tcgtagcgga ttccagcttc 9240 gatctggaag ggcttgtggc ccatctggac gccgcgctgc cttcccatat ggtgccttcg 9300 cgcatcatgc gcctggatca aatgccgctt acgccgaacg ggaagatcga ccgtaaagcg 9360 ctgcctgtac cggaaggaag cattcgtgcc gaggctgcat acacggcgcc tcgtactcct 9420 gccgagcaag cacttgcgtc ggtctggcag tcagtgctgg gcgtggatca ggtcggcacg 9480 atggacaatt tctttgcgct cggcggcgat tcgatcaagg ccttgcaggt atcgtcccgt 9540 cttttgcaaa cggggtacaa gctggtcatg aaagatttgt tccattaccc gacgatttcc 9600 gcccttagtt tgcagctgca aacggcggag agaacggcaa gccaggccga agtgacggga 9660 gaggtcatct tgaccccgat tcagcgttgg ttctttgaac aaaatccggc cgacgtgcat 9720 cacagcaacc aggcgttcat gcagttctcc aagcaaggct ttgacgaaga agctttacgc 9780 caagcggtgc gtcagcttgt cgtgcatcac gatgctctcc gtacggttta ccgccaaact 9840 gagaacggct ataccgcctg gaatcgcggc gccggggaga acgaagcgct gttcgatctg 9900 gaagttgtcg atttcaaggg agtcggcgac gtgaaagaag cggtagaggc taaggcgaat 9960 gacattcaag cgagcatcga tctggaaaac ggtccgctgg tgaagctcgg cttgttccgc 10020 tgcgacgacg gcgaccacct gctcatcgcg atccatcact tggtcgtaga cggcgtatcc 10080 tggcggattc tgcttgagga ttttgctgcc tgctatgagc aggcgctgca agggcagccg 10140 atccgtctgc cgctcaaaac ggattcattc caaacgtggg cgaaacagct cgctgattat 10200 gcgaacggtc cggcgatgga aagcgagaga gagtattggc agcatatcga gcaattgacc 10260 tatgagccgc ttccgaaaga ttttgaacaa ggcagatcca agctgaagga cagcgggctc 10320 gtgaccgttc gctggacggc ggaggaaacc gaacagctgc tgaagcaggc acactgtgct 10380 taccatacgg aaatgaacga tttgctgctt gccgcgcttg gcctcgcggt acaagcttgg 10440 agcggccggg aacgcgtgct ggtgaatctc gaaggccacg gccgggaaga tattttgccg 10500 aatgtggaca ttacgcgcac ggtaggctgg tttacaagcc aattccctgt cgttctggag 10560 ccgggtcacg cccaggcgct cggtcatcag gtaaaacagg ttaaagaaag cttgcgccgc 10620 attccgaaca aaggaatcgg ctacggcatc ctgcgttatt tgtcggcgcc gcgtgacggc 10680 gagcgcttcg ctttggagcc ggagatcagc tttaactatt tgggtcagtt cgaccaggat 10740 tacgaaagca gcggctcgag gccgtctccg ttcagcccgg gctccgattc aagcccggat 10800 gcagtgatgg attatgtcct cgatatcaac ggtatggtgt cggaaggagt gcaggaactc 10860 acgatccgtt atggggaaac ccagtataaa cgggaaacgg tagagcgcct gggcaccctg 10920 cttcattcga gcttgcgtga agtcatcagc cattgcgtat cgaaagagcg gccggagctt 10980 acgcctagcg acgtactgct tcaagatgtg acgttggagg aactggagcg gctggccgaa 11040 catacggcgg cgctcggcga actggagaat gtatacaccc tgactccgct gcaaaaaggg 11100 atgttgttcc acagcctgct ggatgccgat tcggaagctt acttcgaaca ggtgaccttc 11160 gatctgtacg gaagcctgaa tgtcgaagcc ttcacccaag gattggatac gctggtgcag 11220 cggaatgagg cgctgcgtac caactttatt accggctgga gagacgagcc gattcaagtg 11280 gtattccgcg agcggaagtg tgaagtgtac ttcgaagata ttcgctcggt aagcgatgaa 11340 gacccggaga agacgatagc cgatttcgtc agcgcggata aagcgaataa gttcgatttg 11400 gcccgaggct ctcttatgcg cgtgacagtt ttgcgcacgg gcgacgagtc ctaccatgtg 11460 atctggagtc accatcacat tttgatggac ggctggtgca tgtccttcat gatcaaggaa 11520 gtgttcgaca cctacttcgc tttccaggag aagcggacgc tggagcttcc tccggttacc 11580 tcgtactccc ggtatatcga atggctggaa gctcaagatg ccgcgaaagc ttcgcgttac 11640 tggtccgagt atttggcggg ttacgatcag cagaccaagc tgccccagga gaaaacgcag 11700 ctgaagcagg gcgcttttga agcggctgaa atcgatgtgg aactcagcaa ggaactgacc 11760 gggcaaatcg agcgggtggc gcgccagcag caggtgacgc tcaatacgtt catgcagacc 11820 gtatggggac tggttctgca ggtatacaac aacagcgagg atgtcgtatt cggctccgtc 11880 gtatccgggc gtccggcgga aattccgggc atcgaaagca tgatcggcct gtttattaac 11940 acgatcccgg tccgtattca aggcaaagcc gaggagacgg tagccgatat cttgagaaaa 12000 acccaggatc aagcgctggc atcgggagct tacgaaacgt tcccgctgtt cgaaattcag 12060 tcgctgagcg agcacaagcg cgacttgatc aaccatatca tggtctttga aaattatccg 12120 atggaagaac agattgagca ggtcgtcggc ggagacaaag aagcgctgaa aatcgctaat 12180 atccagtcgc cggagcaaac gaactacgac ctggacatta ccgtcattcc ggaagagcat 12240 attttgctgc ggtttacgta caatgcgctg acgttcagag aggaagacat caggctgatc 12300 cacggtcatt ttgcccgggt actggagaag gttgcggcta acccgagcat ccgcgtgaat 12360 cagttggagc ttttgacggc ggcggaaaaa gagcaaattc ttggcgcgtt taacccggcg 12420 cagccggaag cggctccggc ggccgcgttc caccggctgt tcgaggaaca ggcggagcgc 12480 acgccggaag cggcagctgt cgtgtacgag aatgaccggc tgacgtatga ggagctgaac 12540 gagcgggcga atcgcttggc ggccacgctg cgcgcaagcg gcatcggccg ggagacgatc 12600 gtcggcattc tcgccgagcg ttcggtggac ttgctggtgg ccgtgctggc cgtctggaaa 12660 gcgggcgggg cgtatgtgcc gctcgacccg gattatccgg cggagcgggt gcggttcatg 12720 ctcgaagaca gcggagcgaa ggtgctgctg acgcaaacgc cgctgcgaga gcgcgccgaa 12780 gcctggctcg gcgaagagga gctggcgctg gcggcggtgc tgtacctcga cgacgaaact 12840 tcgtacagcg aggagcggac gaatgccgac agcttccatg aagcccgtcc agaggacctg 12900 gcgtacgtga tctacacgtc gggaacgacg ggcaagccga agggcgtgat gatcgagcac 12960 cgcagcctgg tgaacacggc ggcgggctac cggcgggaat accggttgga tcagttcccg 13020 gtgcggctgc tgcagctcgc aagcttctcg ttcgacgtgt tcgtgggaga tatcgcgcgg 13080 acgctgtaca acggaggtac gatggtgatt gtgccgaagg acgaccggat cgatccgtct 13140 cgtctgcacc actggatgga gcgggagcgg gtcaccatct tcgaatcgac gccggcgctg 13200 atcgtgccgt tcctggagta cgtgcacgat cagcggctgg atatgagctg gatggagctg 13260 ttgatcacga gctcggacag ctgcagcgtg gcggattacc ggaccttgca ggaacgcttc 13320 ggctcgttgt tccggatcat caacgcatac ggcgtgacgg aagcggcgat cgactcaagc 13380 ttctatgacg aggagctggc gaagctgccg cagacaggca atgtgccgat cggcaaagcg 13440 tggctgaatg cgaaattcta catcgtggac gcgcatctga acccggtgcc ggtcggggtg 13500 ctgggcgagc tggttatcgg cggagtcggg gtagcgcgcg ggtacttgaa ccgtccggag 13560 ctgacagaag agaagttcgt agacagcccg ttcgccgcgg gcgagcggct gtaccgcacg 13620 ggagacttgg cgcggtggat ggaggacggg aacgtggact tcatcggccg gatcgacaac 13680 caggcgaaaa tccgggggta ccggattgag acgggcgaag tcgaagcgaa gctgctgagt 13740 gtaggtggcg tgaaggaagc ggtcgtcgtc gtcagggaag atcaagaagg tcagaaagct 13800 ttgtgcgctt attatacagc ggaagaaggc ttgacggcgg cggacctgaa gcgggcgatt 13860 gccaacgagc tgccggggta catgatcccg tcgtacttcg tggagctgga gcacctgcct 13920 ctgacgccga acgggaaaat cgaccggaag gcgctgccgg cgccggaagg gggagcaagc 13980 gcaggccgcg aatacgtggc gccgcgcacc gaacttgagg cgaagctggc cgccatttgg 14040 caggatgtgc ttgttcggga gaaggcggta ggcgtaacgg acaacttctt cgacctcggc 14100 ggacactccc tgcgggcgac gacgcttgtc agcaaaatgc ataaggagct aggcgttgaa 14160 tttccgcttc gcgacgtatt ccgctactcg acggttgagg aaatggccgc ggctatggag 14220 cggctggaga tcggctcgtt cacggctatt ccggctgcgg aacctagcga gtattatccg 14280 ctctcttccg ctcagaaacg tctctatatc ttgaaccagc tggaaggagc cgagctgagc 14340 tacaacatac cgggagctat gctgctcgaa ggggagctcg accggcagcg gtttgaagaa 14400 gcgttccgcg ggctcgtagc tcgtcatgaa acgctgcgta ccggctttga gatggtaaaa 14460 ggcgaagcgg ttcagcggat ttacgaagaa gccgccttcc aggtggaata tgtgcagatc 14520 agcgcggagc aggcggaaga aacggtgcgc caattcgttc gtccgtttga tctagcgaag 14580 ccgccacttc tgcgggtggg ccttgccgaa ctggcgccgg accggcacat tctaatgttc 14640 gatacgcatc atatcgtatc tgacggcgtt tcgattgacg tactgatcga agagttcgtc 14700 cgcttgtaca gcggggagca gttggagccg ctccgcattc agtacaaaga ttatgcggta 14760 tggcagcaat cggacgagca gaaagctcag cttgccaagc aggaagccta ctggctcgac 14820 atgttccgcg gagagctgcc ggttttggaa ttgccgatgg actatccgcg cccggctatg 14880 cagagctacg agggccgcac gctgcaattg tttatgcata gcgagaaaag cgagggtctg 14940 aaacggcttg cagccgagaa cggcgcaacg ctttacatgg ttctgcttgc tggttataca 15000 atattattgc ataaatatac agggcaagaa gacgtggtgg tcggtacgcc gattgcggga 15060 agaaatcaca gcgacgttca gccgctgatc gggatgttcg tcaataccct ggccatccgc 15120 agttatccgg ctgccggtaa gacgttcctt gactacttga aggaaatcaa ggagacgacg 15180 ctgggcgctt ttgaacatca gaattatccg tttgaggaac tggtggataa ggtgaacgtg 15240 gctcgcgatt taagccgcaa tccgctgttc gatacgatgt ttgctttgca gaatacagag 15300 aatttggaaa tccagcttcc cggactccat ttgtcgacgt atgccagcga agaaattgtt 15360 tctaaattcg atctcagctt ggacgtcacg gagatcgagg aaggcttgga atatctgttt 15420 gaatacgcca ctgctcttta taaaaccgaa acggtggaga aattggccgc tcactacttg 15480 cagctgcttg aatccattct ccgcaacccg tcggcgacta ttgccgagct gggcattttg 15540 acaccggcgg aaaaagagca aattctcggc gcgtttaacc cggcgcagcc gggagcggct 15600 cctgcggctg cgttccaccg gctgtttgag gaacaggcgg agcgcacgcc ggaagaggcg 15660 gccgtcgtgt acgagaacga ccggctgacg tatgcggagc tgaacgagcg ggcgaaccgc 15720 ttggcggcta cgctgcgcgc aagcggcatc ggccgggaga cgatcgtcgg cattctcgcc 15780 gagcgttcgg tggacttgct ggtggccgtg ctggccgtct ggaaagcggg cggggcgtat 15840 gtaccgctcg acccggatta tccggcagac cgcgtgcggt tcatgcttga agacagtgga 15900 gcgaaggtgc tgctgacgca aacgccgctg cgagagcgtg ccgaagcctg gctcgacgaa 15960 gaggagctgg cgctggcggc ggtgctgtac ctggacgacg aagcgtcgta cagcgaggag 16020 cgggcgaatg cgtcggttgt ctccggcatt gtctccggca ttgtctccgg caagctgacg 16080 gatgctgtgg acgacggcga tgagagccat cagaatgttg gcaccgacag cttccatgaa 16140 gcccgtccgg aggatctggc gtacgtgatc tatacgtcgg gaacgacggg caagccgaag 16200 ggcgtgatga tcgagcaccg cagcctggtg aacacggcgg cgggctaccg tcgggaatac 16260 cggttggatc agttcccagt gcgcctgctg cagctcgcaa gcttctcgtt tgacgtgttc 16320 gtgggagata tcgcccggac gctgtacaac ggaggtacga tggtgatcgt gccgaaggac 16380 gaccggatcg atccgtctcg tctgcaccac tggatggagc gggagcgggt caccatcttc 16440 gaatcgacgc cggcgctgat cgtgccgttc ctggagtacg tgcacgagca gcaactggat 16500 atgagctgga tggagctgtt gatcacgagc tcggacagct gcagcgtggc ggattaccgg 16560 accttgcagg aacgcttcgg ctcgttgttc cggatcatca acgcatacgg tgtgacggaa 16620 gctgcgatcg actcaagctt ctatgacgag gagctggcga agctgccgca gacaggcaat 16680 gtgccgatcg gcaaagcgtg gctgaatgcg aaattctaca tcgtggacgc gcatctgaac 16740 ccggtgccgg tcggagtgct gggcgagctg gccatcggcg gagtcggagt ggcgcgcggg 16800 tacttgaacc gtccggagct gacggaagag aagttcgtag acagcccgtt cgccgcgggc 16860 gagcggctgt accgcacggg agacttggcg cggtggatgg aggacgggaa cgtggacttc 16920 atcggccgga tcgacaacca ggcgaaaatc cgggggtacc ggattgagac gggcgaagtc 16980 gaagcgaagc tgctgagtgt aggtggcgtg aaggaagcgg tcgtcgtcgt cagggaagat 17040 caagaaggtc agaaagcttt gtgcgcttat tatacagcgg aagaaggcct gacggcggcg 17 100 gacctgaaac gggcgattgc cagcgagctg ccggggtaca tgatcccgtc gtacttcgtc 17160 gagctggagc gcctgcctct gacgccgaac gggaaaatcg accggaaggc gctgccggcg 17220 ccggaagggg gagcaagcgc aggccgcgaa tacgtggcgc cgcgcaccga actggaggcg 17280 aaactggtcg ccatctggca ggacgtgctc gggccggtca cgattggcgt gacggacaac 17340 ttcttcgacc tcggcgggca ctccctgcgg gcgacgacgc tggtcagcaa ggtgcacaag 17400 gagctgagcg tggacctgcc gctgcgcgat gtgttccggc actcgaccat cgaagcgatg 17460 gccgaagcga tcagccaatt ggagcggcag gaacacctct ccattccggt tctggataag 17520 agggattact atccgctttc ctccgtgcaa aaacggctgt acatccagca gcagatggaa 17580 ggcgccgagc ttagctacaa catgtccggc atgacggttc tcgtcgggcg cttggaacgg 17640 aatcaattcg aggcggcgct caaaggattg atagctcgtc acgaaatttt gcgaaccggc 17700 ttcgaaatgg tcgacggcga accggtacaa cggatttatc cggacttgaa gtttgccgtc 17760 gagtatacga aagcgacgga aagtgaaacg aagagcatcg tagacggctt tgtgcgcgtc 17820 tttgatttgg agcggccgcc gctgctgcgt gtgggcttag tcgaatggga agcggaacgg 17880 catctgctca tgctggacat tcatcatatc gtcacggatg gcatgtcgat gggcatcttc 17940 gtcgaagagc tgctgcgcct gtataacggc gagactatgg aaccgcttcg gattcaatac 18000 aaggaattcg ccgcttggca gcagtccgaa cctgtaaaag agcggctgaa acgtcaggaa 18060 gcctactggc tggacgtgct ggaaggcgaa ctgccgacgc ttgaactgcc tacggacttt 18120 gtcagacctg ccgctcgcag ctttgaggga gatgtgctgc ctttcagcat cgacaagcag 18180 atgaccgaca gcttgcagcg catcgccgat gagaacggtg ccacccttta tatggtgtta 18240 ttggcggcct attcaatcct gctcagcaag tactcgggac aagaagactt cattgtaggc 18300 acgccggttt cgggccgcac acatgccgac ctggagccgc tcatcggaat gtttgtcaac 18360 actttggcga ttcgccatta tccgtccggg gagaagacgt tcctcgctta cttgaacgaa 18420 gtcaaagaaa cgatgctggg ggcctacgac caccaggatt atccgttcga ggagcttgtg 18480 aaaaagctgc aggcgccgcg agatcaaggc cgcaatcctg tattcgatgt catgtttgct 18540 ctggaaacca aggaagataa cgttcaaagc ttcggggata tcaagatcga atcttatccg 18600 gaaactcata cggtttccca atttgatctt accttggtca tttcgttgct ggatgaggga 18660 atgaacgggc agtttgaata tgccaccaag ttgtttacac gcaatctgat cgacaatttc 18720 gctcaggacc tgctcgtgat catcacccaa atttgcgaac agccttcggt gctgttgaag 18780 gatatttccc tgaacgggca atccgaacag gagcaagatg tgctagagac cattgatatt 18840 attttctaa 18849 <210> 10 <211> 1827 <212> DNA <213> Paenibacillus polymyxa F4 <400> 10 atggaagctg accgccagcc gtctgttcta gaacaaggca cagcggcgcc ttccaagcgc 60 caaaccgctt acgccgcctg gaaagcgttc cgctggctga tgtcctatgt aagccgtcac 120 aaaggctgga tgatcgtcgg tatcttgtcc gcgattgccg ccgccgttat tgagatatgg 180 acgggaagtt tgatcgagca gctgaccacc caggccgaga agggggcggg gccaatcgtt 240 ctgcaaatcg tgtacacggt ctttgtggtc atcttgatcg gtgtgccggc gaagtttttc 300 atgagcttcg gcgtggagcg aagcagcgcc tctgcggtgc aggatatccg caaccatgtc 360 atgcgtcata tcggcaaact cccggtctcc tatttggaaa agcagcactc cggcgacgta 420 ttgtcgcgga tcaacaacga cctgcagctc atccagcagt ttatgattcg ggacctcgcc 480 cagtggtttt atcatccgct gttattcatc ggctgtttcg cttatttgat ctacctccaa 540 tgggagctga tgctgtacag cctgctgtta tttcccgtag cgctgctggt ttcccaatgg 600 atcggcaagc agttggagcg gttgacggag gaagcccagg cgaacatggg ccgaatgaac 660 gtcaacctcc aggatacgct tgggggtatg cctattgtaa aaagctacct gctatccggc 720 atgttatctc gctcctacca agtgctgctg caattgacgg cccaaaaaaa gctggccgtg 780 aaaaagcggg aagcctgggt caacccgctg ctttccacgc tgatgatcag cccgatcatt 840 ttcgccgtca gttacggaag ctatttgatc tacaaagggc agctaggcgc gggagagctg 900 atcgccttcc tgtacttgct gaatctgtgt ctggagccgc tggagcatat tcccgagctc 960 atcacgcgga cgttcgaaat ggccggtgcc ctgagaaggg tctccgaaat cgtcgagcag 1020 ccgaccgaaa cggaaaatgg ccgttcgctt ccgaaagcga gcgccgcccc catcgagttt 1080 cagaacgtaa ccttcgggta tgaggagagc tccccgatcc tgcggaatgt tagcttctcg 1140 gtgccggaag ggaagacgat cgcgcttgtc ggagcgagcg gcggagggaa gagcacggtg 1200 tttaagcttg tatgcggctt ttatccgctt ccggaggacc agggcgagat ccgcgtgttc 1260 ggcagcctga tccacggcgc cgatccggag cagcttcggt cgcatttttc cgtggtaacc 1320 caggattcat atttgtttag cggcacgatc gctgaaaata tcggctacgg gcgggaagta 1380 gcgtcgatgg acgagattat cgaagccgcc aaagccgctc aggcgcattc cttcattatg 1440 cagcttcccg acggctacca gacgtatgtc ggagagcgcg gaggcttttt gtccggcggg 1500 cagcgccagc gcattgcaat ggcccgggct tttctgaagg atgctcccgt tctgctgctg 1560 gacgagccaa cgtcggctct tgatccggag tcggaaagcg cggttcagga agcgctgggc 1620 gtattgatga agcagagaac gacgatggtt attgcccacc ggctttctac agtacaaaac 1680 gccgacgaaa tttgggtcat ggaacagggg agtattgttg aaaagggcac tcatgagcaa 1740 ttgctgaaga tgaagggact gtacgcccag tcatactatc aggaatttac cgagtctgcc 1800 gaacgcaggg aggtggcgta cacatga 1827 <210> 11 <211> 1734 <212> DNA <213> Paenibacillus polymyxa F4 <400> 11 atgaaaaagg gcggatggct ctcgcaagtg aaagaactcg gctacttgct gatgtttatg 60 aaccgcaagc gcaaaaccca gtacgccatt ggcctggccg tgacggcgct cacccagaca 120 ttgttcctga tcgccttcag cttggtcgta cataacttgg ttgatttcgc cgtgtcccga 180 gatacgtccc tgatggtgga agcctttatt attttgggcg cggccttgtt cctggaaaat 240 gtgatttctc cctggttcat ttacttatac cagcgcagtg tcgagctgac tgtgctgaat 300 atccgcgaac gtctttatga caagctgtgc cgggtgcgac cgagattcct ggagcagacg 360 caccatgggg acttgctgtc gcgggtgaac aacgacgtaa cgaccgttga gtttacattc 420 tcgcaggttt acttcgtttt gctgcttcaa gttgtctttt gcatcggctc cattgtctcc 480 atggtgttga tcgattggcg gtttgccggc gtatccttcg tcattctgct gctgtcctcc 540 gtggtcagcc tgaaatttgc gcgggatatt cgcgccttgt ccgaacaagg cttgcaaacg 600 ctcggtaaaa tgaccgaaaa attcaaagat tttatgggcg gcattcaaat tgtgaagctg 660 ttccgcatcc gcacgattta cggccagtac gaggcgttga acgaacaaat gacgcagacg 720 cttcggcaaa ccgcgcagaa gaacggcacg caggctgcgg tgaatcattt tatcagctac 780 gtcacgttct gcggcatcat cgtcatcggc agtcttcttt atgcctacgg actgatggga 840 atgggaagcg tcgccgccct ggcggttctg caagtgaatc tgacgcacgc cttgttgaac 900 ttggggatgg ttctgtcgat gacccaaaat tcgctcgcgg gcgctcaccg gattcaagag 960 gtactaagag aggaagagga accggagcgt ttgggatctc ctcacagcga gctcgtgtcg 1020 gaggctgcgg tggagtttcg cgatgtggta ttttcctatc aggcggataa aaaggtgctt 1080 atcgacatct ccatgcaggt gtttcccggc caggtcgccg ctatcgtggg ggccagcggc 1140 agcggcaaaa gtacgctgat caagctgctg ctcggctttt atcctgtgga cagcggagaa 1200 atcctgctcc aaggcaaacc gttcggccat tacacgctgg acgaaatccg caggcagatt 1260 gcatacgttc cgcaggagcc gttcttgttt accggcacga ttgaggaaaa catccgctac 1320 ggcaacccgg atgcaacgga tgaagaagtg atcgaagcgg ccaaagcggc gtacgctcac 1380 catttcattc aggaacttcc tgaacagtat aaaacgccgg tgggagagag aggagcgtcg 1440 ttgtcgggcg gacaaaggca gcgaattgcg atcgcccggg cgattctcaa aaacgccccg 1500 attctgctgc tggacgaagc gacttccgcg ctggataacg aatcccagca ttgggtacag 1560 caggccctga acgaattgat gaaggggcgc accaccattc tgatcgccca ccgtctcagc 1620 accgtggaac atgcggattt gattaccgtc atgaaccaag ggacggtcgt cgagcggggc 1680 cgccatcagg acctgctggc gctcgggggg tattacgccc ggctgtacgg ctag 1734 <210> 12 <211> 14994 <212> DNA <213> Paenibacillus polymyxa ATCC21830 <400> 12 atgtttgaaa aggcggagag cgaggctgga gcgttgctgc tgcatatgct ccgccggatt 60 gcttttaccg atgaagagag gggaacgatc cagatggctt ttgaaaaaga aacgttgttt 120 tggaacgaaa aattcggtag cgacgattat accttgacgc ggctgcctta cagcaaagct 180 ccaagctccc tgacgcccat tatgacaacc gtcggcggga cgctttcgga ggaagtggcg 240 cagcgcgtcc ttcaaatgag caagggcgct ccgctggcca cttttatgat tttgctcgca 300 ggtgttcagt ccctcttgta taaatataca ggcgcttctg acattctggt cggcatgccg 360 gttgtacgga aaccgacgga gacgcgccga tccgttaatc atacgatcat tttgaaaaac 420 tcgctttcgg cgggtgcgac ttttaaaacg cttctgagcg agttgaggac ttccttgccg 480 gaagcgattc agcatcaaca tattccgttc ttgaaaatga cggagaagct ggatctgcaa 540 tatgcagacg ggatacccgt cgtccatacg ctagtatccc ttaaggagct gcacctggac 600 gaaatcggac aaaacgtggt cacggattgt tcctttgaat tcagcttaac gggcgggacg 660 atacagctag cgttgtcata taacgagcac ttatatgact ccgagttcat gactcgggtc 720 gttggccatc tgaatcgact gttggccgtg gggcttcacg agttggagct ggagatcgtg 780 cgggcggaca tgctgtcgga ggacgagaaa tttcaattgt tgcaaagctt taacgatacc 840 gagaaggact atccccgtga tcggacgatt catcaactcg tggaggagca ggcgaagcgg 900 gtgcccgaag cgacggcggt tgtctttgag ggtcggcggc tttcgtacgc ggagctgaac 960 gaacgggcga accggctggc gcggacgctg cgatcggtcg gcgtgctgcc caatcagctg 1020 gtaggcttga tggccaggag atcactggag acggtcgttg gcatactggc ggttctgaaa 1080 gctggcggtg cctacgtgcc gatcgacccg gaatacccgg aagaacgcat ccgctacatt 1140 ctggagaact cgaacgcgca gctgctgctg actcaaaggg agctgctgca gcaggtgccg 1200 ttcgaaggaa ccgtgttggc gctggatgac gaacagacct acagcgacga tggctcgaac 1260 ttggagccgg ccagcggtcc gaatgatctt gcttatgtca tctatacgtc aggtacgacg 1320 ggcaaaccca aaggggtcat gctggagcat cgcggtttgg tcagcttgaa gctgatgttc 1380 gcggataggc taggcatcac ggagcatgac cggatcgttc aattcgccag cctgtcgttc 1440 gacgcgtcct gctgggaaat gttcaaagcg ctctattttg gcgcggcttt gtatatcccg 1500 acggccgaga cgattctcga cacccgcttg ttcgagagtt atatgaacga gcatgcgatt 1560 acggcggcga ttttgcctcc aacgtacagc gcttatttga acccggaccg ccttcccagc 1620 ttaacgaagc tcgtaacggg aggctctgcg gtatcggccg aattcgtgca gcaatggaaa 1680 ccgaaggtcc actatttcaa tgcttacggc cctaccgaag cttcgattgt tacgacgctt 1740 tgggatgcgg atgaggagca gtcggagcgc agagtcattc cgatcgggcg cccgctggcc 1800 aatcaccgga tttttatttt ggatgcccac ctgcagcttg tgcctccggg agtggacggc 1860 gagctgtgcg tggccggcgt ggggcttgcg agaggttacc tgaaccatcc ggagctgacg 1920 gcagagaagt tcgtggaaca tccgtttgcg ccgggagaac gcctttaccg gacgggagat 1980 ctcgcccgct ggctgccgga cggaaatatt gagtacttgg gccggatcga tcatcaggtg 2040 aaaatccgtg gattccggat cgagatcggc gagattgaag agcaacttct gaagatcgac 2100 tccgtgcagg agacgatcgt aatcgcgcgg gaaggcaaaa gtggacaaga actgtgcgct 2160 tacctggtcg cgggccaccc gcttacgctc ggcgagttga gaagcgcgct ggcgcaaaaa 2220 ttgccgaatt acatgattcc ggcgcatttt gttcagcttc cgcggatgcc gctcacgccg 2280 aacgacaaaa tcgaccgcaa ggctttgccc gtcccggaag gaaacgcact gaccggcggc 2340 gcgtacgtag ctccccgcaa tgaagccgag cggacgcttg ccgatgtgtg gcaggcggta 2400 ttgaacgccg atcgcgttgg ggtaacggat catttcttcg agctgggcgg agactcgatc 2460 aagtccattc aagtatcttc gcggcttcat caagccgggt acaagctgga aatccgggat 2520 ttgttcaaat atccgactat ctcacagctc agtctgcatg tgaaaccgat cggacgtacc 2580 atcgatcaag gcgaaataac gggtgaaacg gcgctgacgc cgattcagca ttggtttttc 2640 gagagctcct ttgcggaccc gcatcatttc aaccagtcgg tgatactgta ccggaaggaa 2700 cgcttcgacg aagagacggt gcgtcaggtg ctgcaaaagc tggccgagca tcatgatgcc 2760 ttgcggatgg tgttccgcaa aacggaacaa gggtttagcg cgcggaaccg cgcgattcaa 2820 gaaggcgggc tgttcacgct ggacgtgttc gacttcaagg atgcaaagaa taccgcgcag 2880 gctgtggaag cgaaggcaac ggacattcaa gcgggcatcg atctggagaa cgggcccctc 2940 gtgaaggcag gactgttccg atgcgcggac ggcgatcatt tgctgctcgc ggttcatcat 3000 gccgtggtgg acggcgtgtc ttggcgtatt ttgatggagg atttcgccct gggttacgag 3060 caggccggca aaagtgagga aattcgtttc ccggcgaaaa cggatgcgta ccgcacttgg 3120 tccgagcagt tggccgctta cgtgcaaagc ccggagatgg cgaaggaacg ggcctattgg 3180 caggccgtgg aacaaattgc ggttccggcc gtgccgaagg atctggaggc ggacgttacg 3240 acgcagcagg acagcgaatc gctgttcgtc tgtttgactc ccgaagaaac ggaactgctg 3300 ctgaagcggg ttcaccgggc ctacaacacc gaaatgaacg atattttggt aacggcgctc 3360 ggcatagcca ttcgcaagtg gacgggacac gaacgggtgc ggatcaatct cgaaggacac 3420 ggacgcgaat cgatcggaac ggatatcgac atcacgcgca cagtcggctg gtttacgacc 3480 aagtttccgg tcgtcctgga gccggaaacc gaccgggatt tggcctatca gattaaacag 3540 gtcaaggaaa gcttgcgccg cattccgaac aaagggcttg ggtacggcgt atgccgctat 3600 ctctccaaat cggaggatgg ctttgtttgg ggtgcagagc cggaaattaa ttttaactac 3660 ctcggccagt tcgacgatga tgtcaaccag gacgagatcg gcatatcttc ttattccagc 3720 ggcagcccgg ccagcgaccg gcaggcccgc agctttgtgc tggatatcaa cggcatggtg 3780 ctggacggcg ctttatcgct cgatctcagc tacagccgga agcagtatcg caaggaaacg 3840 atagaagcct tcgctcagcg gcttgagcaa agtctccgag agctcattac ccactgcgca 3900 ggcaaagaaa acaccgaatt gacgccgagc gacgtgcaat ttaaaggctt gaccatcgcg 3960 gaattggagc aaatcgccca gcgctcgggc catctcgggg aaatcgaaaa tatttactcg 4020 cttacgccga tgcagaaggg catgtggttc cacagcgcgc ttgaccggca gacggccgct 4080 tacttcgagc aaacgcggtt tacgatgcgg ggagcactcg acgtccagct tttcgagagg 4140 agctggatgg agcttgcgaa acgtcatctg gtgctgcggg caaattttgt gaaaggaccg 4200 gcgggcgagc cgctgcaaat catataccgc gacaagccgg tcggctttga atatgaagag 4260 ctgctccatt tgcaggcgga cgagaaacaa acgtacttgg ataaaaaggc cgaggatgac 4320 aagcttcgcg gcttcgacct ggaacatgac gcgctcgttc gggttacgat cctgcgcacc 4380 gaagagcaaa gctatcatgt gctgtggagt ttccagcata ttttgatgga cggctggtgc 4440 ctgccgcagc tgacgcagga gctgtttgag acgtattcgg ccttggcatc cggcaagcag 4500 ccagcgggag ataagggatc ggattatggc gcttatatcg aatggttgga gaaacaggac 4560 gatcaggcgg catccggcta ttggacggca ttcctggcag gttatgaagg gcaaaccgta 4620 ctcccaggac aaaaggaacc ggcgccaaac ggcagattta cggctgatca cgtcaccgcc 4680 gagctcggca aggacttgag cgagcgaatg gaccgggtgg cgaaacagcg cctggttacg 4740 gtcaatacgc tgctgcaagc cgcttggggc gtgatgctgc aaaaatataa cgggacaaac 4800 gatgccgtat tcggcagcgt cgtagccgga agaccggcgg aaatcccggg catcgagtcc 4860 atgatcgggc tgttcatcaa tacggtgccg gtccgcgtca cgagcgaagc ggacaccgtg 4920 ttcgccgacc tgatggcgaa gctccaagag cgggcgctgg agtctgggcg ttatgattac 4980 tatccgctgt atgaaattca agcccacagc gtgcaaaagc agaacctgat caaccatatc 5040 atcgctttcg agaactatcc ggtggacgag cagatggagc aggcgggcga ccagcagcac 5100 ggcgacctga cgatcgctga cgttcagatg gaggagcaga cgaactataa cttcaacgtg 5160 accgtggtgc cgggagacga gatcgaaatt cggttcgact ttaacgcaga ggtgttcgat 5220 aaagacagca tcgagcggct caaggggcat ctcgtccatc tgctggagca ggtgacggat 5280 aacccggaaa ttacagtagg cgagctggaa cttgtgacgg aggcggaaaa ggccgacctt 5340 ctcggacgtt ttaacgacac taccacggaa tttccgcgcg ggaagacgct cattcaattg 5400 ttcgaagagc aggcggagcg catcccggat gcggctgcca tctccttgaa tgagcaagag 5460 ctgacctacc gcgagctgaa cgaacgcgtc aatcgccttg cccgtacctt gcgtagccac 5520 gggatatcca aaggccgtct ggtcgccatt atggctgagc gttccatcga aatggtggtg 5580 ggtatgctgg cggcacacaa agccggagcg gcttacgtac cgattgaccc ggaatatccc 5640 gaggagcgta tccgtttctt gatcgaggat tcgggagcgc aggtcatgct gacgcaaagc 5700 cgcttgcgcg agcgcctggc gggttcggac tccgtgatct tactggatga cgagtccttc 5760 tatcacgagg acggcacaaa tcttaatccg ggcaccgaag cgacagatct ggcctgcgtc 5820 atctatacgt caggtacgac gggcaagccg aaaggcaacc ttgtttcgca ccgcaacatc 5880 gtgcggatcg tgcgaaatac gaattatatc gacatcaccg agcgggatca tgtcctccag 5940 ctttcaagct attcgttcga cggagcgatt ttcgatgttt tcggcgcttt gaccaatggg 6000 gcgcggctgg tgctggttcc ccgcgagact ttgctggaaa tcggccggct ggcggatctc 6060 atccagcacg agcgcatctc ggtcatgctg attacgacgg cttacttcaa cgtccttgta 6120 gatgtgaacg tcgactgcct gcgggatgtc cgggcgattt tgtttggagg agagcgcgtg 6180 tcggtcggcc atgtgcgtcg agcgctcgcc catatcggac cgggcaggct taaccacctg 6240 tacggcccgt cggaaagcac ggtttatacc acgtatcttc cggtcgactt tgtcgatgag 6300 tcggcggtta ccgtacccat cggacggccg atcagcaata caacggtgta tatcgtcgac 6360 agccggaata agcttctgcc gatcggcgtg gctggagagc tttgcgtcgg cggagaaggc 6420 ttggtacggg gctacaacaa ccggccggag ctgacggcgg agaaatttgt cgacaatccg 6480 tttgtgccgg gagagcgcat gtaccgaacg ggggatttgg ccaaatggct gccggacggc 6540 acgatcgaat acgtgggacg gacggacgac caagtgaaaa tccgcggctt ccgcattgaa 6600 ctgggcgaga tcgaagctca gcttcagaaa gtggagggaa ttcggaaaac gacggtattc 6660 gcgagggaaa acgcctccgg cgagaagcag ctttgcgcct attatgaagc ggaccgcgag 6720 ctttcagcgg ccgagttgaa gaacgtgatt tcccaggaac tgccggccta catgatcccg 6780 gcgtacctga tccagttgga gcggctcccg ctgacggcga acggcaaggt cgaccgccga 6840 tcccttccga cgccggagga gagcttgcag ccgggcgaag gacgtactcc gcctcgtact 6900 ccgctggaag ccagcttggc cggaatttgg aaaagcgtac tcgcattgga gcacatcggc 6960 gttcatgaca acttcttcga cctgggcggc cattccctgc gggcgacgac actggtgagc 7020 aaggtgcatc aggagctgaa cgtcgagctg cctctgcgcg acgtattccg ctactcgacg 7080 atcgaagaga tggctctcgc catcacccgg atcggagagc agtcattctc gtcgattccg 7140 ctggcaggcg taagagaata ttatccgctt tcctcagctc agaagcggct gtttatcctg 7200 aatcagctag aagggtccga tcagagctac aacatgccgg gcgtgctgct gctggaagga 7260 tcgattgacc ggagcctgct ggagaaggct ttccgcggac tgatcgtacg gcacgaaacg 7320 ctacgaaccg gctttgagat cgtacaaggc gaagcagtac agcgcattta cgagagcgtc 7380 gactttgccg tcgagtaccg tcatgcgagc gaggaagaag cgcctgaagt cgtgcaggcc 7440 ttcatccgac ctttcgactt ggcgaagcct ccgctgctgc gggtggagct cgtagagctc 7500 gcaaccgaac gttatttgct gatgttcgac atgcaccata tcgtctccga cggggtttcg 7560 atggacgtgt tagtcgagga actcgttcgt ctgtacggtg gcgagtcatt agagcctttg 7620 cgcattcaat acaaggacta tgcggtatgg cagcagtcgg acgagcaaaa agcgcagttg 7680 aaacgcgagg aagcttactg gctggaccgt taccggggtg aactgccggt tctggaaatg 7740 ccgacggact atccgcgtcc tgccgtgcag agctttgagg gacaaacgct gacgtccttc 7800 gtggacgagg caacgaatga aggcttgaag caactggccg ctcaaagagg aacgacgctg 7860 tatatggtgc tgcttgcggc atatacagtg cttttgcata aatacacagg tcaggacgat 7920 ttgatcgtcg gaacgtcgat tgcgggcaga acgcacggag acacgcagcc tttgatcgga 7980 atgttcgtca atacgctggc gctccgcaat tatccggctt cggagaagac cttcctgtcg 8040 tatctggaag aagtgaaaga aacgaccttg ggtgcttacg agcatcagaa ttatccgttc 8100 gaagagcttg tcgataaagt gcaggtcagc cgggatttga gccgcaatcc gctgtttgac 8160 acgatgttct ccctgcaaaa cttggaggat aaagagtttg agctggaagg gctgaaattg 8220 tccccgtacc ctagcgaata cggtacggcc aagttcgacc tgagtgtgga tgttacggaa 8280 gaaaacggcg gcctggagtg cagctttgaa ttcgcaacgg ctctttataa agaaagcacg 8340 atccggcggt tgtcgactca tttcggacat ttgcttgcgg agatcgtaag ctgtccggat 8400 gcgaagattg ccgagctgaa cttgctgacg gcagaggaaa aagaacaaat tctcggcgcg 8460 tttaacccgg cgcagccgga agtggctcct gcggccgcgt tccaccggct gtttgaggaa 8520 caggcggagc gcacgccgga agcggcggcc gtcgtgtacg agaacgaccg gctgacgtat 8580 gcggagctga acgagcgggc gaaccgcttg gcggccacgc tgcgcgcaag cggcatcggc 8640 cgggagacga tcgtcggcat tctcgccgag cgttcggtgg acttgctggt ggccgtgctg 8700 gccgtctgga aagcgggcgg ggcgtatgtg ccgctcgacc cggattaccc ggcggagcgc 8760 gtgcggttca tgcttgaaga cagcggagcg aaggtactgc tgacgcaaac ggcgctgcga 8820 gagcgtgccg aagcctggct cggcgaagag gaactggcgc tggcggcggt gctgtacctg 8880 gacgacgaag cgtcgtacag cgaggagcgg gcgaatgcgc cgattgtctt cggcatttcc 8940 agcaagctga cggatgctgt ggacgacggt gatgagagcc ctcagaatgt tggcaccgac 9000 agcttccatg aagcccgtcc ggaggatctg gcttacgtaa tctacacgtc gggaacgacg 9060 ggcaagccga agggcgtgat gatcgagcac cgcagcctgg tgaacacggc ggcgggctac 9120 cggcaggaat accggttgga tcagttccca gtgcggctgc tgcagctcgc aagcttctcg 9180 tttgacgtgt tcgtgggaga tatcgcgcgg acgctgtata acggaggcac gatggtgatt 9240 gtgccgaagg acgaccggat cgatccgtct cgtctgcacc attggattga gcaggagcgg 9300 gtcaccatct tcgaatcgac gccggcgctg atcgtgccgt tcctggagta cgtgcacgaa 9360 catcggctgg atatgagctg gatggagctg ttgatcacga gctcagacag ctgcagcgtg 9420 gcggattacc ggaccttgca ggaacgcttc ggctcgttgt tccggatcgt caacgcatac 9480 ggcgtgacgg aagcggcgat cgactccagc ttctacgacg agccgctggc gaagctgccg 9540 cagacaggcc atgtgccgat cggcaaagcg tggctgaatg cgaaattcta catcgtggat 9600 gcgcatctga atccggtgcc ggtcggggtg ctgggcgagc tggtcatcgg cggagtcgga 9660 gtggcgcgcg ggtacttgaa ccgtccggag ctgacggaag agaagttcgt agacagtccg 9720 ttcgccgcgg gcgagcggtt gtaccgcacg ggagacttgg cgcggtggat ggaagacggc 9780 aacgtggact tcatcggccg gatcgacaac caggcgaaaa tccgggggta ccggattgag 9840 acgggcgaga tcgagtcgca gctgctgcgg gtgaaaggcg tgcgcgaagc ggtggtactg 9900 gttctaagtg acgcgaacgg gcagaaggcg ctgtgcgcgt attacacgcc ggataccgga 9960 gcggagctgg cagtgaacga tctgcgcagc acgctggcgc aggagttgcc ggggtacatg 10020 atcccgtcgt acttcgtgga gctggagcac ctgcctctga cgccgaacgg aaagattgac 10080 cggaaggcgc tgccggcgcc ggaaggggaa gcgggaaacg gaacggagta cgtcgcaccg 10140 cgcaatgagc tggaaacaaa gctggcggcg atttggcagg aggtactggg gcttgcgaag 10200 gagattggcg ttcacgacaa cttcttcgac atcggcggcc actccctgcg ggcgacgacg 10260 ctggtcagca agattcataa agagttgaac gtggatctgc cgctgcgcga cgtgttccgc 10320 cattccacca tcgagagcat ggcggccgcc atttcccggc tggatgagca gacattcgtt 10380 tccataccgg tggcggatga ccgagaggtg tacccgcaat cttttgctca aaaacgtctc 10440 tttatcctga atcaactgga aggcgcggag cttagctaca acatgccgga agcgatgctg 10500 ctggagggtg ctttggaccg ggcaaggttc gaagaagcgt tccgtaagct cgtgacgcgg 10560 catgaaacgc tgcgcaccgg gttcgaaatg gtggatggcg aagcatcgca gcggatttat 10620 caggacgtga atgttgccgt ggagttctat cgagcggata agcaagaggc cgaagagacg 10680 gttcgtcgtt tcgtccgtcc gtttgacttg gcgaagcctc cgctgctgag ggtaggcctt 10740 gtcgagctgg ctccggaacg ccatattcta atgtacgaca tgcatcatat tatttccgac 10800 ggcgtctcga tggaaatctt tgttgaagaa ttcgtccgct tgtacggcgg cgagcaattg 10860 gatcctctgc gcattcagta caaagactac accgtttggc agcattcgca ggagcagaag 10920 gaacggcttc agcgccagga ggcatactgg ctggacatgt tccaaggcga gcttccggtg 10980 ctggaaatgc cgaccgacta tccgcgtccg gccgtgcaga gctacgaagg ccaaacgctg 11040 gagtttttct tcgacgcttc gaaaaccgac ggcctgaagc agctggcctc ggaaacgggc 11100 acgacgctgt ttatggtgct gcttgcggcg tataacgtcc ttttgcataa atattcaggt 11160 caggaagacg tgatcgtcgg tacaccgatt gctggaagga atcatggaga tgtacagccg 11220 ttgatcggga ttttcttaaa cacgctggct atccgcagtt atccggcttc ggagaagaca 11280 ttcctgtcat acctgaacga agtcaaagaa acgaccctcc acgccttcga gcatcaaaac 11340 tatccgttcg aagaattggt ggacaaggtg caagtcaccc gtgatttaag ccgcaatccg 11400 ctcttcgaca cgctgtttac gatgcagaat acggagaacg aggaatttga gctggaaggg 11460 cttcgcctga ttccttatcc gagcgcactg gataccgcga agtttgatat cagcttggat 11520 gtgggcgagg agaacggcgg cttggattac agcttcgaat atgcgacggc tctctacaaa 11580 agggagacga tcgaacggct ggcgaaacat tacgaacagc tgctcgtaac gatcgtaagc 11640 cgtccggatg cgaagatcgc cgagctgaat ttgctgacgg cagaggaaaa agaacaaatt 11700 ctgggcgcgt tcaacccggc gcagccggaa gcgacttctg cggctgcgtt ccaccggctg 11760 ttcgaagaac aagcggagcg cacgccggaa acggcagctg tcgtgtacga gaatgaccgg 11820 ctgacgtatg cagagctgaa cgagcgggcg aaccgcttgg cggccacgct gcgcacaagc 11880 ggcatcggcc gggagacgat cgtcggcatt ctcgccgagc gttcggtgga cttgctggtg 11940 gccgtgctgg ccgtctggaa agcgggcggg gcgtatgtgc cgctcgaccc ggattacccg 12000 gctgagcgcg tgcggttcat gctcgaagac agcggagcga aggtgctgct gacgcaaacg 12060 gcgctgcgag agcgcgccga agcctggctc ggcgaagagg agctggcgct ggcggcggtg 12 120 ctgtacctgg acgacgaaac ttcgtacagc gaggagcggg cgaatgcgcc gattgtctcc 12180 ggcatggtct ccggcaagct gacggatgct gtggacgacg gcgatgagag ccatcagaat 12240 gttggcaccg acagcttcca tgaagcccgt ccggaggatc tggcatacgt gatctatacg 12300 tcgggaacga cgggcaagcc gaagggcgtg atgatcgagc accgcagcct ggtgaacacg 12360 gcggcgggct accggcggaa ataccggttg gatcagttcc cagtgcggct gctgcagctc 12420 gccagcttct cgtttgacgt gttcgtggga gatatcgcgc ggacgctgta taacggaggc 12480 acgatggtga ttgtgccgaa ggacgaccgg attgatccgt ctcgtctgca ccattggatt 12540 gagcaggagc gggtcaccat cttcgaatcg acgccggcgc tgatcgtgcc gttcctggag 12600 tacgtgcacg aacatcggct ggatatgagc tggatggagc tgttgatcac gaactcagac 12660 agctgcagcg tggcggatta ccggaccttg caggaacgct tcggctcgtt gttccggatc 12720 gtcaacgcat acggcgtgac agaagcggcg atcgactcca gtttctacga cgaggaactg 12780 gcgaagctgc cacagacagg caatgtgccg atcggcaaag cgtggctgaa tgcgaaattc 12840 tacatcgtgg acgcgcatct gaacccggtg ccggtcgggg tgctgggcga actggtcatc 12900 ggcggaatcg gagtggcgcg cgggtacttg aaccgtccgg agctgacgga agagaagttc 12960 gtagacagcc cgttcgccgc gggcgagcgg ctgtaccgca cgggagactt ggcgcggtgg 13020 atggaagacg gcaacgtgga cttcatcggc cggatcgaca accaggcgaa aatccggggg 13080 taccggattg agacgggcga gatcgagtcg cagctgctgc gggtggaagg cgtgcgcgaa 13140 gcggtggtgc tggttcgaag tgatgcgaac gggcagaagg cgctatgcgc gtattacacg 13200 ctggataccg gagcggagct ggcggtgaac gatttgcgca gcgcgctggc gcaggagctg 13260 ccggggtaca tgatcccatc gtacttcgtg gagctggggc gcctgcctct gacgccgaac 13320 ggaaagattg accggaaggc gctgccggcg ccggaagggg aagcgggaag cggaacggag 13380 tacgtcgcac cgcgcaatga gctggaaacg aagttggcgg cgatttggca ggaggtgctg 13440 gggcttgcga aggagattgg cgttcacgac aacttcttcg acatcggcgg ccactccctg 13500 cgggcgacga cgctggcggg caaggtattt aaggaattaa acgtcaacct gccgctgcgc 13560 gacgtattcc gtcactcgac gattgcggcg atggctgagg cgatcgcccg gatggaacgg 13620 cgggagcatg aggctattcc tcaagcggag gagagagagt actaccctct gtcctccgcg 13680 cagaaacggc tgttcattca gcatacgctg gatggagcgg atcagcttta caacatgccg 13740 gagttggtgc aggtggaagg cgagtttgat ttagaccggt tggaagccgc cttgcggaaa 13800 ttgataacac ggcatgaatc gctgcgcacc ggttttgaaa tcgtgaaggg cgaagcggtt 13860 cagcgggttt acccgcaggt cgattttgct atcgagcatc atcaagcgga taaagaggat 13920 gcggctcaaa tcgagcagat cgtccgcagc ttcattcgtc cgtttgatct cggcaagccg 13980 ccgctgctgc gcgccggggt catcgagctg gagccgaacc tgcatattct ccttttcgac 14040 atgcaccata tggtgtctga cggcgtatcg atggcgattg tgatcgatga gttctcaagt 14100 ttctacgccg gggaagaact gccgccactg cgcattcaat acaaggatta tgccgtttgg 14160 cagcagtcga aggcccaccg agagcggatc gggcggcagg aagcgtactg gctgcaaacc 14220 ttcgaaggcg agctgccgac ggcggacctg ccgatcgact acgaacggtc cgcggctcgc 14280 agctacgaag gcgcgcatct ggagttcaac gtcgaagctt ctctctctgc gcggctgcgc 14340 gaattggcgg ccgagcgtga aagcacactg ttcatggtgc tgcttgcggc ttataccgtg 14400 ctgctgtcca agtacagcgg gcaggaggac ttgatcgtgg gtaccccggt ggcgggaaga 14460 acgaacgccg atttggaatc ggtcatcggg atgtttgtca atacgctggc gatccgcaat 14520 cgtccttcgg gcgacaagac gttcttgtcc tacctggaag aagtgaagga aacggctttg 14580 ggtgctttcg agaaccagga ctatccattc gaggagctcg tggagcgttt gaatgtgaag 14640 cgggagccgg gccgcttccc gctgttcgat gccgttttcg acttgcaaaa tatcgaagaa 14700 cgagacgccg agctggaagg ggtcagcctg aagacttacg agcttgacca tttagaagaa 14760 gcgaagttcg atctgacact gtttatgtat gaaaacaacg gggcgctgag cgggggcttc 14820 ttctacgcca ccaagctgtt caaagaagcg atgatccgca ccttgaccga ggattacctg 14880 cgggtactgt ctcaaattgc ggaaaatcca caactcgagc taagccggat tgaatgccat 14940 aaaccggcgg caggcgcaaa gagtgccgtc gatacgatcg aattcgcgtt ctaa 14994 <210> 13 <211> 3309 <212> DNA <213> Paenibacillus polymyxa ATCC21830 <400> 13 atgaaatctt tgtttgaaaa ggaagaacgg tactggagcg gcaagtttga cgccgatgac 60 agcctgagct tccttcccta cagtcaatcc tccaaattat cggccgacgg ggaagctgcg 120 gccgagccgg gtttgcttca ccgtactctg ccgagcgaaa tctcggagag aatcatttgc 180 cttgccaacg gttcggattt ggctttgtac atgattgttt tagcaggagt aaaaagcctg 240 ctgttcaaat ataccgggcg ggaccaagtg ctggtcggga tgccctctta tagcgcagac 300 cccgacggga ctccgccgcc gcatgacatc ttggtgatca agacgtccgt aagccgcgag 360 actacgctta gaacgctgct cgggggcatc aaagcctcca tcggcgaggc gctggagcat 420 cagcacctgc cttttcggaa aatggtggag ccgctccatc tggactatac gggggacggt 480 ctcccggtcg tcaacaccgt cgcatccttc gccccgattc atccggaacc gctgggtaac 540 cgggtggcgg ccgatacggt ttttcgcttc gaccgccaaa accactccat cgagctggaa 600 ataagctttg acgggcagcg gtacgagcgg gcatttgtag aacaggcggc cgaccatctt 660 gttcagctgc tgtccgtgct tttatttcag ccggatctgg agcttggaca agccgatgtg 720 ctgtccccag acgagaggga gacgctgctg aagcgattta atgacaccga aaccgggttc 780 gagcggggga aaacgattta cggcttgttc gaagaacagg cggagcttta cccggacaac 840 gtggccgccg tcatgaacga gcggcagctg acctaccgcg agctgaacga gcgatccaac 900 cgccttgcgc ggaagctgcg ggagacggga gtagaagcgg accagctggt agcgattctg 960 gccgaacgct cgctcgatat ggtcgtcggc attctggcga ttctcaaagc gggcggagca 1020 tacgtgcctg tcgatcccga ctacccggag gagcgcatcc gcttcatgat cgaggattcg 1080 ggtgcgccgt tattgctgat tcaaaagcat ctgcacgaga agaccgactt cgcaggaacg 1140 cgcctcgaat tggacgattt cgtttggggt gacagagggg cggactccga aggagcgctg 1200 gacgcttcga acctggagcc gatttctggg ccagtcaacc tggcttatgt catctacacg 1260 tcgggaacga ccggcagacc aaaaggaacg ctgatcgagc ataagaacgt cgtgcgcctc 1320 ctgttcaacg acaagaacct attcgacttc gggccgtccg acacgtggac gctgttccac 1380 tcgttctgct tcgatttctc cgtctgggaa atgtacggag cgctgctgaa cggaggcaag 1440 ctggtcatcg taccgccgct cacagcgaaa aatccggccg atttcctggc gctgctgggc 1500 cgcgaacagg tcacgatttt gaaccagacg ccaacgtact tctaccagct gctgcgtaag 1560 gtcttggcgg accatccgta cgatttgcgg attcgcaacg tcatcttcgg gggcgaagcg 1620 ctgagtccgc tgctgctcaa gggcttcaag acgaagtacc cggagacgaa gctgatcaat 1680 atgtacggca ttaccgagac gacggttcac gttacgtata aggaaatcac gtgggtcgaa 1740 atggaggcgg cgaagagcaa tatcggcaag ccgatcccta cgctgagggt gtacgtcctt 1800 gatgaaaacc gccggcctgt gccgatcggc gtagcgggcg aaatgtacgt ggccggggca 1860 ggccttgcga gaggatacct gaaccgtccg gatctgacgg cggagaagtt cgtcgattcc 1920 ccgtttgcgg agggggagaa actgtaccgc tcgggtgact tggcggcttg gctgccggac 1980 ggcaacatcg aatacctggg ccggatcgat catcaggtga aaatccgcgg gtaccggatc 2040 gagctggacg aaatcgagac gcagctgctg aagatcgccg ccgttcaaga agccaaggtg 2100 ctcgaccgcg acgacgcaaa cggccaaaag cagcttgtcg cttactacgt cgcggaaacg 2160 aggctggcgg tgcatgaact caaggaggag ctcgccaagc agcttccggg gtatatgatt 2220 ccttcgcacc tcgtgcagct tccgcggatg ccgctgaccc cgaacgggaa aatcgaccgc 2280 aaagcgctgc ccgcgccgga ggaagccgcg gcccgaggag cggaatatgt cgcgccgaga 2340 acgctgctcg aaatgaagat cgcccgcgtc tggcaggata cgcttggcgt tccgcaggtc 2400 ggcgtaaagg ataacttttt tgagttgggt ggcaattcgt taagtctgat gaggctcgtt 2460 caagccgttt acgatgaaac gggcattgag ataccgctaa accgccaatt ccataatgta 2520 acagttgaag ccatggcttt cggagagggg gacctgggcc tggataaagg gggagactcc 2580 ttcattaagc tgaataaagt aggaaatctg aatgtgttct gcttccctcc gggcagcggc 2640 ttcggcatcg gttaccgaga gctcgcaagc aggctcgacg gtcggtccgt gctctacagc 2700 attgatttta tcgacgatgc cgtcgattac gaggccatgc tgaaccgtta tgtggacgag 2760 atcgtccgca tccagccgga aggaccttac gtgctgctcg gctactgctt cggaggaaac 2820 ctgacgttcg aggtagccaa aacgatggag aaaagagggt attccgtaac agacgtgctt 2880 atggtggact cgtggattaa gcacacgctg acgccttccg aaacgtcgga gaaagagctt 2940 gaagaaacgc ttgccgattt cgacgaagaa gagaaggaat taatgagcaa cccgctcgta 3000 cgggagcggg ttcatcggaa ggtcaaagcg accttggcgt acgaagcgca gctcattaat 3060 tccggcacga tcccggcccg gatttacgaa ctgattgcga aggacagcga agcgttccgc 3120 ctggagcacc aattgccttc ctggcggggg gcaacgacgc aagcttacgc cgattaccgg 3180 ctggagggcg cgcacgagga attgctggaa ctcgcgcgcg tggacgaaac ggccgtcgtc 3240 atccgggaca tcttagagca agtcaagcgg cagattgaag cggaggccgg tgtactgcat 3300 ggaagctga 3309 <210> 14 <211> 17370 <212> DNA <213> Paenibacillus polymyxa ATCC21830 <400> 14 gtgagagagg ataccaacga gcaatatgga ttaacgcaag cccagcgccg aatatggttc 60 atggaaatta tgaatccggg aacgtccatc acgatgcttt ccgcgaccta ccagattacg 120 ggcgagatca acacacagct tctgaagcaa gcggcggcag agatcgtcaa aacctatgac 180 gttttccgaa tccgcattag cggggatttg caaaatccaa cgcagtggtt cgaagagccg 240 gagaatgtcc aagctaggat aagccgcctc gaaataggca caaccgaaca attctatgct 300 tgggtgaaag aagtaagcga aaaaccggcc agcgtgttcg acgaacacct ctaccaattt 360 acgattatcc attttgcgaa cggccaagta tggctcaatt tgacggtaaa tcatattatc 420 gccgacggct tgtccgtcaa tgctttgctg catgcggtga tggaaaagta cctggaactg 480 cgcaaaggca tctccagcag ttaccagccc ccttcctatc tagattatat ttcctcggag 540 cgtgaatatg agcaatcgca gcgttatcaa aaaggcaagg aatactggct gacaaagtac 600 aacactttgc ctgaaacgac cggcattaaa tcgtatccgc cattctcgat cggcagcgaa 660 tccaataaac tgtccatcac tttggacggt tcccggtatg aacgcattct ggccttctgc 720 gaacaatatc aggtcagctt atatacgtta tttctgtccg ccatgtacgc cttattgtac 780 aaactgaccg acagcaccga tgttccggtc ggcacggttt tcgccaatcg caccagcaag 840 aaggaaaaag aaacgatcgg catgttcgtc agcaccgtgg ctacgcggat ttgtctgaat 900 ccagacgggc acgtgctttc cttgatccaa acggtttcca aggaaaatac ggcggatctg 960 cggtatcaga aatatcctta taaccaattg atccaggatt tacgcgaaca acacggccgc 1020 aacgatcttt cggggctgtt ccgcacgtct ctggaatatc tgcctttgaa aatcgtggag 1080 tacgaagaaa tcaaggtacg cttggaggct cactttgcta ggcacgagat ggacgatttg 1140 ctgctgcgct tcgaccatat gctgaatgaa agccatgtaa ttctccatgc ttcctatcgt 1200 accggcctgt tcgagaaggc cgagattgat cggattatgg aacagtacgt aaccgttctg 1260 gaccagtttc ttcagactcc cgaactgccg gtacgcgaga tttctctgct gagcgatgat 1320 gagaaacacc gcattctcaa cgtttttaac ccgccggtgg cagggctgag cgagggagaa 1380 gcgtttcatc ggtatgttga aaagtttgcc cgggaaattc cggatcatcc ggcagtcgtc 1440 tacatggaca aacagctgac ctacagcgaa ttgaacgaac gcgccgagcg gctggcttct 1500 ctccttcgcg aacagggcgt gggaagggag acgattacgg gtatctgggc ggagcgttcg 1560 gtggaactgc tggtcggggt gctcgccgtt tggaaagccg gcggagccta tgtaccgctg 1620 gaccccgatt atccagcgga gcggattgag tatatgctca gcgatagcga tgcatcggtg 1680 ctgcttacgc agcgtcatct gttggagcgg gccggaggtt ggttggccga tgaccggctg 1740 aagcttcaag ctgtctatgc catggacgat gaacagattt ataacgggga tgccttagcc 1800 gtggaatttg aatctgccga cagcgccccg caagacttgg cttatgtgat ttacacctcg 1860 ggtacgacgg gacgcccgaa aggcgtcatg attgagcacg gtagtctcgt gaatacggcg 1920 gatgcgtacc gtcgcgagta ccggttggat cagtttccgg tgcggctgct gcagctggcc 1980 agcttctcgt ttgacgtgtt tgtcggagac atcgcgcgga cgctatataa cggaggcacg 2040 atggtgattg tgccgaagga tgaccggatt gatccgaacc tcttatacgg ctggattcgg 2100 gaccaaaaca ttacggtatt cgaatcgacg cctgcgctca tcctgccgtt catgcagcat 2160 atttatgaag aagggctgga cgttagctcc atgcagttgc tgattaccag ctcggatgct 2220 tgcagtgtca ccgattaccg attgctgcag gaaagattcg gcggacaatt ccgcatcatc 2280 aacagctatg gcgttaccga agcggccatt gacagcagct tttacggtga gccgctggat 2340 aagttgccgc cgtcgggtca tgtgccgatc ggcaaagctt ggctgaacgc ccggttttac 2400 attgtcgatg ctgcgttaaa gccggttcct gtaggggttc cgggcgagct tgtcatcggc 2460 ggcgccgggg tggcgcgcgg gtactggaac cgtccggatt taaccgccga gaagtttgcg 2520 gacagcccgt ttgtgccggg cgaacgtctg taccggacag gcgatttggc ccgctggcta 2580 gaagacggta acgtcgactt catcggccgg atcgactatc aggtgaaaat tcgcgggttc 2640 cggatcgaac tcggcgaaat tgaaacggcc ctgctgcgtt tcccgggcgt caagcaggct 2700 gtggtgaccg accgtacgga tgagcagggg caaaagtatt tgtgcggcta cgtggtagag 2760 gatgcttccg tgcagctgag cgatctgctg tcccaattga agcaagagct gccggcccat 2820 atggtcccgg cccggctggt gtctcttgat aagcttccgc tcacgccgaa cggcaaaatt 2880 gaccgtaaag cgctgcctga accgaccgga gaggtagaag caggccatga gtatgtggct 2940 cctcgcacaa cgctggaaac aagacttgct ctcatttggc agcaggtgct gggtattgcg 3000 cgagttggag tccaagacga tttctttgac ttgggtggtc attccttgcg ggcctccacg 3060 ctggtttcaa agattcggaa agagctgcaa gtcgaggttc cgctgcggga agttttccgc 3120 tacaccacga tcgaacagct ggcccaaaga atcggcggtt taaggcagca ggagacgtat 3180 gagattacaa aggcggctga ggccgagtac tatccggttt catccgagca aaagcgtctg 3240 tacgtcctgc gccagcttga cggggccgag cgcagctaca atatgtcggc ggcgcttctt 3300 ctcgaaggca agctggaccg cacgcgcgtc gagcacgcgt tccgggcgct gattcagcgc 3360 catgagacgc tgcgtaccgg gattgagcag gttcaaggcg agcttgtcca gcgcatctat 3420 gacgagttgg attttgctgt agattatttc caagcgagcg agcgggaagt ggagcaagtg 3480 gtggaagctt actatcaccc gtttgatctg actaagccgc cacttctccg catcggtctg 3540 atcgaagtcg ccgaggatcg ccacattctg ctgttcgata tgcaccatat cgtctcggac 3600 ggcatttcga cagcgctgct cttcgacgag ttcagccgcc tgtatcgggg cgaggagctg 3660 gccccgctgc gcattcaata caaagattat gccgtttggc agcattccga agcttacggg 3720 cagatgctcc agccgcagaa ggagtattgg ctagaacagc tgtcgggcga gctaccggtc 3780 ttggagctac cgacggactt cccgcgacct gcggtgcaaa gctttgacgg ccgaaccgtg 3840 aagttttata tcgagaaaga tcggacggag aagctgaaag aactagcggc acggacaggg 3900 acgaccctgt acatggtgct gctgtcggct tacaccatcc ttatgcataa atattcgggt 3960 caggaagatc tgatcgtagg aacgccgatt gccggaagaa cgcaggaaga agtgcagccg 4020 atcgtaggga tgtttatcaa cacgctggcc attcgcagcc gtccggagcg ttccaagccg 4080 tacctttcgt acctggaaga aatcaaggac atcacgctcg gggctttcga acaccaaaat 4140 tatttgttcg aagacttggt agaaagtctt cacattccgc gcgcgaccgg ccgtaatccg 4200 ctctttgaca cgttcttctc cctacaaaat acggagaacg agcaaattgt catcgagggg 4260 ctggagcaat cgttttatcc actggaaaat caaacatcca agttcgagct gctcctggat 4320 atttcggagc tggacggtca gctcgaatgc cggttggagt acgctacggc tttgtataaa 4380 caagagaccg cggagcggtt cgccagacat tatgacaagc tgctcgaaac catcgcagca 4440 gcgccggacg gggatattgc ctcgctggaa atgctcacgg aggaggaaat ccgcgagctg 4500 atgcgcggtt tcaacgattc agaggcggac tacccgcggc agcagacgat tcacggcttg 4560 ttcgaagagc aggcggagct ttacccggac aacgtggccg ccgtcatgaa cgagcggcag 4620 ctgacctacc gcgagctgaa cgagcgatcc aaccgccttg cgcggaagct gcgggagacg 4680 ggagtagaag cggaccagct ggtagcgatt ctggccgaac gctcgctcga tatggtcgtc 4740 ggcattctgg cgattctcaa agcgggcgga gcatacgtgc ctgtcgatcc cgactacccg 4800 gaggagtgca tccgcttcat gatcgaggat tcgggtgcgc cgttattgct gattcaaaag 4860 catctgcacg agaagaccga cttcgtagga acgcgcctcg aattggacga tttcgtttgg 4920 ggcgacagag gggcggactc cgaaggtgcg ctggacgctt cgaacctgga gccgatttcc 4980 gggccgctca acctggctta tgtcatctac acgtcgggaa cgaccggcag accaaaagga 5040 acgctgatcg agcataagaa cgtcgtgcgc ctcctgttca atgacaaaaa cctgttcgac 5100 ttcgggccgt ccgacacgtg gacgctgttc cactcgttct gcttcgattt ctccgtctgg 5160 gaaatgtacg gagcgctgct gtacggaggc aagctggtca tcgtaccgcc gttcacggcg 5220 aaaaatccgg ccgatttcct ggcgctgctg ggccgcgaac aggttaccat tttgaaccag 5280 acgccaacgt acttctacca gctgctgcgc gaggtcttga cggaccatcc gtacgatttg 5340 cggattcgca acgtcatctt cgggggcgaa gcgctgagtc cgctgctgct caagggcttc 5400 aagacgaagt acccggagac gaagctgatt aatatgtacg gcattaccga gacgacggtt 5460 cacgttacgt ataaggaaat cacgtgggtc gaaatggagg cggcgaagag caatatcggc 5520 aagccgatcc cgacgctgag ggtgtacgtc cttgatgaaa accgccggcc tgtgccgatc 5580 ggcgtagcgg gcgaaatgta cgtggccggg gaaggccttg cgagaggata cctgaaccgc 5640 ccggatctga cggcggagaa gttcgtcgat tccccgtttg cggaggggga gaaactgtac 5700 cgctcgggcg acttggcggc ttggctgccg gacggcaaca tcgaatacct gggccggatc 5760 gatcaccagg tcaaaatccg cgggtaccgg atcgagctgg acgaaatcga gacgcagctg 5820 ctgaacgtcg agggcgtgga agaagcggtg gtgcttgccc gtcaggatgg cgggggcgag 5880 aaggcgcttg tcgcctactt tgtggcggat cggacactga cggtcagcga aatgagaacc 5940 tcgctggccc agggaatgcc ggggtacatg atcccgtcgt acttcgtgca gctggagcgc 6000 atgccgctga cggccaacgg caaagtagac cgcaaagcgc tgccggagcc gcaaggcagc 6060 ttgcagacgg gcgtcgaata tgtagcaccg cgtaaccgga tggagtccca gcttgtgaag 6120 atctgggagg aagtgctggg ctactccggc attggagtcc tggacaattt cttcgagctc 6180 ggaggccact ccttgcgggc gacgaacctt gtcagcaaga ttcagaagga aatgaacgtc 6240 gaacttccgc tgcgcgatgt gttccgctat acgacggtag agtcgatggc cggggctatt 6300 gccagcttgg aagaaacgcg gcacaactca attccgaaag cggaagagaa agcgtactat 6360 ccggtttcct ccgcgcaaaa aaggctgtac gtcctgcacc agctggatgg ctcggagctg 6420 aattacaacc tcccaagtgc cttgcagttg gaaggggctt tgaacgaagc caaagtggaa 6480 aaggcgctga ctactttggt ggcccggcat gatatgctgc gcaccggttt tgaaattgtg 6540 aatggggagc cggttcagcg tattcatccg atcgtagctt tcaaggtcga aaagcttcaa 6600 gcaagcgaag atcaagttgc ggccattctt gaaggcttca ttcagccttt tgacttgacc 6660 cagccgcctt tgctgcgcgc cctgctgatc gaactggaga aagagaaatt cctgctcgcg 6720 ctggatattc atcatattgg ttccgacggc ctttccatgg acgtgctgct gcgcgaattc 6780 gtgcggcttt acaatgggga agaattgccg gagctgcgga ttcaatacaa ggattacgcc 6840 gtttggcagc aatccgagga gcagcgccag cgcatcaaac ggcaggagga atactggcgt 6900 ggggtattca gctccgagct tccggttctt gagctgcctc tcgacttctc ccgcccggcc 6960 gtccagcagt ttgacggtca aacgctcacg tttacgctgg atgcggagaa aagcgaagct 7020 ctcaaacggc ttgccggcga ttcgggggca acgctgtaca tgcttctgct ggctgcgtac 7080 tccgtattgc ttcataaata cgcgggacag gaagatatag tggtcggcac tccgattgcc 7140 gcccgttctc acgccgacct gcagccgatt atcggtatgt tcgtcaatac gctggccctt 7200 cgcttgggtc cggcggcgga gcggacgttc ctggattact tgcaggaagt gaaggaaaca 7260 acgcttggag cctacgagca ccaagactat ccgttcgagg agctggtgga agctcttcag 7320 gtgagccggg atttaagccg gaatcctctg tttgacacca tgttttcttt gcaaaagcac 7380 gaaagcttgg atttaaccct ggaaggcttg caatggtcgc tgttcgacat cgaggaaaag 7440 acggcaaagt ttgaccttag ctttgatatc gtggaagccg ataacgaact ggtttgcaag 7500 atcgagtacg ctacctcgtt gtttagacag gaaacggtgg tacggctggc aggtcattac 7560 gagcagcttt tgacgtcgat cctggctcag ccgggtgcgc ggatttcgga tttggatgtc 7620 ttgacggaca gcgaaaaaca tgatttgctg gtcgggtttg acgtgtcgtc ttcggctctt 7680 gcgaagcaac ccgccgcaga gggtacaggt ttggaagcgg atgagtcgtg gagagagaga 7740 acgttccacg agctgttcga ggagcaggcg gagcgcactc cggaagcgct ggcggttgtc 7800 tacgaagaca gcaagttgac gtatgcggag ctgaacgcca aagcgaatcg tctggcgtat 7860 gcactgcggg cgcgcggggt gaagccggag caggtggtcg gcattctggc cgatcgttcg 7920 gcggagctgt tgatcggggt gctcgccgta tggaaagcgg gcggcgctta tgtgccgctc 7980 gacccggact atccggcgga gcggatcgag tatatgctcg cggacagcgg ggcgtcggtg 8040 ctgcttacgc agacccgcct gctggagcag gcggaagctt ggcgcagcga cggagctcta 8100 gtgctgcaaa cggtgcttgc gcttgacgac gtcgcgacgt acagcctcgg agcggcggaa 8160 gcggctgtgg gtgtacaagc tttgggcgaa gcaggcgcag aggcggaggc tttggcgcaa 8220 gcggctgctg ccgaaacgtc cgccacggca gaagccgaga agaacgtact agcggcagat 8280 ctcgcatcga atccggcgaa tgtgaacaag ccgcgcgatt tggcttacgt catctacacc 8340 tccggtacga cgggcaagcc gaagggcgtg atgatcgagc accgcagcct ggtgaacaca 8400 gcggcgggct accgtcggga ataccggttg gatcagttcc cggtgcggct gctgcagctc 8460 gcaagcttct cgttcgacgt gttcgtggga gatatcgcgc ggacgctgta caacggaggc 8520 acgatggtaa ttgtgccgaa ggacgaccgg atcgatccgt ctcgtttgca ccactggatg 8580 gagcgggagc gggtcaccat cttcgaatcg acgccagcgc tgatcgtgcc gttcctagag 8640 tatgtgcacg agcagcggct cgatatgagc gggatggagc tgttgatcac gagctcggac 8700 agctgcagcg tagcggatta ccgcaccttg caggagcgct tcggctcgca gttccgtatt 8760 attaacagct acggcgtaac ggaagcggcg attgactcca gcttctatga cgagcctctg 8820 gaaaagctgc cgaagacggg cagcgtgccg atcgggaaag cgtggctgaa cgccaagttc 8880 tacatcgtgg atgcgaatct gaagccggtg ccgatcgggg tgttgggcga gctggttatc 8940 ggcggagtcg gagtggcgcg cgggtacttg aaccgcccgg atttgacggc ggagaaattc 9000 gtagacagcc cgttcgccac tggggagcgg ctgtaccgga caggcgacct ggcgcgctgg 9060 atgtcggacg gcaacgtgga ctttatcggc cggatcgaca accaggtgaa aatccggggg 9120 taccggattg agacaggcga agtcgaagcg aagctgctga gtgtaggtgg cgtgaaggaa 9180 gcggtcgtcg tcgtcaggga agatcaagaa ggtcagaaag ctttgtgcgc ttattataca 9240 gcggaagaag gcttgacagc ggcggatctg aagcgggcga ttgccagcga gctgccgggg 9300 tacatgatcc cgtcgtactt cgtggagctg gagcgcctgc ctctgacgcc gaacggaaag 9360 atcgaccgga aggcgctgcc ggcaccggaa gggggggcag gaggccgcga atacgtggcg 9420 ccgcgcaccg aactggaggc gaagctggcc gccatttggc aggatgtgct tgttcgggag 9480 aaggcggtag gcgtaacgga caacttcttc gacctcggcg ggcactccct gcgggcgacg 9540 acgctggtca gcaaaatgca taaggagcta ggcgttgaat tcccgcttcg cgacgtattc 9600 cgctactcaa cggttgagga aatggccgcg gctatggagc ggctggagat cggctcgttc 9660 acggctattc cggctgcaga atctagcgag tattatccgc tctcttctgc tcagaaacgt 9720 ctctatatct tgaaccagct ggaaggagcc gagctgagct acaacatacc aggagctatg 9780 ctgctcgaag gggagctcga ccggccgcgg tttgaagaag cgttccgcgg gctcgtagct 9840 cgtcatgaaa cgctgcgtac cggctttgag atggtaaaag gcgaagcggt tcagcggatt 9900 tacgaagaag ccgccttcca ggtggaatat gtgcagatca gcgaggagca ggcagaagaa 9960 acggtgcgcc aattcgttcg tccgtttgat ctagcgaagc cgccccttct gcgggtaggc 10020 cttgctgaac tggcgccgga ccggcacatt ctaatgttcg atacgcatca tatcgtatct 10080 gacggcgttt cgattgacgt actgatcgaa gagctcgtcc gcttgtacag cggggagcag 10140 ttggagccgc tccgcattca gtacaaagat tatgcggtat ggcagcaatc ggacgagcag 10200 aaagctcagc ttgccaagca ggaagcctac tggctcgaca tgttccgcgg agaactgccg 10260 gttttggaat tgccgacgga ctatccgcgc ccggctatgc agagctacga gggccgcacg 10320 ctgcaattgt ttatggatag cgagaaaagt gagggtctga aacggcttgc agccgagaac 10380 ggcgcaacgc tttacatggt tctgcttgct ggttatacaa tattattgca taagtataca 10440 gggcaagaag acgtggtggt cggtacgccg attgcaggaa gaaatcacag cgacgttcag 10500 ccgctgatcg ggatgttcgt caataccctg gccatccgca gttatccggc tgctggtaaa 10560 acgttccttg actacttgaa ggaaatcaag gagacgacgc tgggcgcttt tgaacatcag 10620 aattatccgt ttgaggaact ggtggataag gtgaatgtgg ctcgcgattt aagccgcaat 10680 ccgctgttcg atacgatgtt tgctttgcag aatacagaga atttggaaat ccagctcccc 10740 ggactccatt tgtcgacgta tgccagcgaa gaaattgttt ctaaattcga tctcagcttg 10800 gacgtcacag agatcgagga aggcttggaa tatctgtttg aatacgccac tgctctttat 10860 aaaaccgaaa cagtggagaa attggccgct cactacttgc agctgcttga atccattctc 10920 cgcaacccgt cggcgacgat tgccgagctg ggcattttga caccggcgga aaaagagcaa 10980 attcttggcg cgtttaaccc ggcgcagccg gaagcggctc ctgcggcggc gttccaccgg 11040 ctgttcgagg aacaggcgga gcgcacgccg gaagcggcag ctgtcgtgta cgagagcgac 11100 cggctgacgt atgcagagct gaacgagcgg gcgaaccgct tggcggccac ggtgcgcgca 11160 agtggcatcg gccgggatac gatcgtcggc attctcgccg agcgttcggt ggacttgctg 11220 gtggccgtgc tggccgtctg gaaagcgggc ggggcgtatg tgccgctcga cccggattat 11280 ccggcggagc gcgtgcggtt catgctcgaa gacagcggag cgaaggtgct gctgacgcaa 11340 acgctgctgc gagagcgcgc cgaagcctgg ctcggcgaag aggagctggc gctggcggcg 11400 gtgctgtacc tggacgacga agcgtcgtac agcgaggagc gggcgaatgc gccgctggaa 11460 gcggatgatc acttgcacga atgccgtccg gaggacctgg cgtacgtgat ctacacgtcg 11520 ggaacgacgg gcaagccaaa gggcgtgatg atcgagcacc gcagcctggt gaacacggcg 11580 gcgggctacc ggcgggaata ccagttgaat cagttcccgg tgcggctgct gcagctcgcc 11640 agcttctcgt tcgacgtgtt cgtgggagat atcgcgcgga cgctgtacaa cggaggcacg 11700 atggtgattg tgccgaagga cgaccggatc aatccgtctc gtctgcacta ctggatcagc 11760 cagcagcaag tgacgatctt cgaatcgacg ccggcgctga tcgtgccgtt cctggagtac 11820 gtgcacgagc atcaactgga tatgagcggg atggagctgt tgatcacgag ctcggacagc 11880 tgcagcgtgg cggattaccg caccttgcag gaacgcttcg gctcgttgtt ccggatcatc 11940 aacgcatacg gcgtgacgga agcggcgatc gactccagct tctacgacga ggagctggcg 12000 aagctgccac aaacaggcaa tgtgccgatc ggcaaagcgt ggctgaatgc gaaattctac 12060 atcgtggatg cgcatctgaa tccggtgccg gtcggggtgc tgggcgagct ggtcatcggc 12120 ggagtcggag tggcgcgcgg gtacttgaac cgtccggagc tgacggaaga gaagttcgta 12180 gacagcccgt tcgccgcggg cgagcggctg taccgcacgg gagacttggc gcggtggatg 12240 gaagacggga acgtggactt catcggccgg atcgacaacc aggtgaaaat ccgggggtac 12300 cggattgaga caggcgaagt cgaagcgaag ctgctgagtg taggtggcgt gaaggaagcg 12360 gtcgttgtcg tcagggaaga tcaagaaggt cagaaagctt tgtgcgctta ttatacagcg 12420 gaagaagact tgacggcggc ggacctgaag cgggcgattg ccagcgagct gccggggtac 12480 atgatcccgt cgtacttcgt ggagctggag cgcctgcctc tgacgccgaa cggaaagatc 12540 gaccggaagg cgctgccggc accggaaggg ggagcaggcg gaggccgcga atacgtggcg 12600 ccgcgcaccg aactggaggc gaagctggcc gccatttggc aggatgtgct tgttcgggag 12660 aaggcggtag gcgtaacgga taacttcttc gacctcggcg ggcactccct gcgggcgacg 12720 acgctggtca gcaaaatgca taaggagcta ggcgtggaat tcccgcttcg cgacgtattc 12780 cgctactcga cggttgaaga aatggccacg gctatggagc ggctggagat cggctcgttc 12840 acggctattc cggctgcgga acctagcgag tattatccgc tctcttccgc tcagaaacgt 12900 ctctatatct tgaaccagct ggaaggagcc gagctgagct acaacatacc aggagctatg 12960 ctgctcgaag gggagctcga ccggcagcgg tttgaagaag cgttccacgg gctcgtagct 13020 cgtcatgaaa cgctgcgtac cggctttgag atggtaaaag gcgaagcggt tcagcggatt 13080 tacgaagaag ccgccttcca ggtggaatat gtgcagatca gcggggagca ggcggaagaa 13140 acggtgcgcc aattcgttcg tccgtttgat ctagcgaagc cgccccttct gcgggtaggc 13200 cttgctgaac tggcgccgga ccggcacatt ctaatgttcg atacgcatca tattgtatct 13260 gacggcgttt cgattgacgt actgatcgaa gagttcgtcc gcttgtacag cggggagcag 13320 ttggagccgc tccgcattca gtacaaagat tatgcggtat ggcagcaatc ggacgagcag 13380 aaagctcagc ttgccaagca ggaagcctac tggctcgaca tgttccgcgg agaactgccg 13440 gttttggaat tgccgacgga ctatccgcgc ccggctatgc agagctacga gggccgcacg 13500 ctgcaattgt ttatggatag tgagaaaagt gagggtctga aacggcttgc agccgagaac 13560 ggcgcaacgc tttacatggt tctgcttgct ggttatacaa tgttactgca taagtataca 13620 gggcaagaag acgtggtggt cggtacgccg attgcaggaa gaaatcacag cgacgttcag 13680 ccgctgatcg ggatgttcgt caataccctg gccatccgca gttatccggc tgccggtaaa 13740 acgttccttg actacttgaa ggaaatcaag gagacgacgc tgggcgcttt tgaacatcag 13800 aattatccgt ttgaggaact ggtggataag gtgaatgtgg ctcgcgattt aagccgcaat 13860 ccgctgttcg atacgatgtt tgctttgcag aatacagaga atttggaaat ccagctcccc 13920 ggactccatt tgtcgacgta tgccagcgaa gaaattgttt ctaaattcga tctcagcttg 13980 gacgtcacag agatcgagga aggcttggaa tatctgtttg aatacgccac tgctctttat 14040 aaaaccgaaa cagtggaaaa attggccgct cactacttgc agctgcttga atccattctc 14100 cgcaacccgt cggcgacgat tgccgagctg ggcattttga caccgtcgga aaaagagcaa 14160 attcttggcg cgtttaaccc ggcgcagccg gaagcggctc ctgcggcggc gttccaccgg 14220 ctgttcgagg aacaggcgga gcgcacgccg gaagcggcag ctgtcgtgta cgagagcgac 14280 cggctgacgt atgcggagct gaacgagcgg gcgaaccgct tggcggtcac gctgcgcaca 14340 agcggcatcg gccgggatac gatcgtcggc attctcgccg agcgttcggt ggacttgctg 14400 gtggccgtgc tggccgtctg gaaagcgggc ggggcgtatg tgccgctcga cccggattat 14460 ccggcggagc gcgtgcggtt catgctcgaa gacagcggag cgaaggtgct gctgacgcaa 14520 acgctgctgc gagagcgcgc cgaagcctgg ctcggcgaag aggagctggc gctggcggcg 14580 gtgctgtacc tggacgacga agcgtcgtac agcgaggagc gggcgaatgc gccgctggaa 14640 gcggatgatc acttgcacga atgccgtccg gaggacctgg cgtacgtgat ctacacgtcg 14700 ggaacgacgg gcaagccaaa gggcgtgatg atcgagcacc gcagcctggt gaacacggcg 14760 gcgggctacc ggcgggaata ccagttgaat cagttcccgg tgcggctgct gcagctcgcc 14820 agcttctcgt tcgacgtgtt cgtgggagat atcgcgcgga cgctgtacaa cggaggcacg 14880 atggtgattg tgccgaagga cgaccggatc gatccgtctc gtctgcacca ttggattgag 14940 caggagcggg tcaccatctt cgaatcgacg ccggcgctga tcgtgccgtt cctggagtac 15000 gtgcacgaac ataggctgga tatgagctgg atggagctgt tgatcacgag ctcggacagc 15060 tgcagcgtgg cggattaccg caccttgcag gaacgcttcg gctcattgtt ccggatcatc 15120 aacgcttacg gcgtgacgga agcggcgatc gactccagct tctacgacga ggagctggcg 15180 aagctgccgc agacaggcaa tgtgccgatc ggcaaagcgt ggctgaatgc gaaattctac 15240 atcgtggacg cgcatctgaa cccggtgccg gtcggagtgc tgggcgagct ggtcatcggc 15300 ggagtcggag tggcgcgcgg gtacttgaac cgtccggagc tgacagaaga gaagttcgta 15360 gacagcccgt tcgccgcggg cgagcggctg taccgcacgg gagacttggc gcggtggatg 15420 gaagacggca acgtggactt catcggccgg atcgacaacc aggcgaaaat ccgggggtac 15480 cggattgaga cgggcgagat cgagtcgcag ctgctgcggg tggaaggcgt gcgcgaagcg 15540 gtggtgctgg ttcgaagtga tgcgaacggg cagaaagcgc tgtgcgcgta ctacacaacg 15600 gatggggaac tgacggtggc agacctgaaa cgggcgatct ccagcgagct gccgggttac 15660 atgatcccgt cgtacttcgt cgagctggag cgcctgcctc tgacgccgaa cgggaaaatc 15720 gaccggaagg cgctgccggc gccggaaggg ggagcaagcg caggccgcga atacatggcc 15780 ccgcgcaccg aactggaggc gaaactggtc gccatctggc aggatgtgct cggaccggtc 15840 acgattggcg tgacggacaa cttcttcgac ctcggcgggc actccctgcg ggcgacgacg 15900 ctagtcagca aggtgcacaa ggaactgagt gtggacttgc cactgcgcga agtgttccgg 15960 cactcgacca tcgaagcgat ggccgaggcg atcagccaat tggagcggca ggaacacctc 16020 tccattccgg ttctggataa gagggattac tatccgcttt cctccgtgca aaaacggctg 16080 tacatccagc agcagatgga aggcgccgag cttagctaca acatgtccgg catgacggtt 16140 ctcgtcgggc gattggaacg gaatcaattc gaggcggcgc tcaaaggatt gatagctcgt 16200 cacgaaattt tgcgaaccgg cttcgaaatg gtcgacggcg aaccagtaca acggatttat 16260 ccgaacttga agtttgccgt cgaatatacg aaagcgacgg aaagtgaaac gaagagcatc 16320 gtagacggct ttgtgcgcgt ctttgatttg gagcggccgc cgctgctgcg tgtgggctta 16380 gtcgaatggg aagcggaacg gcatctgctc atgctggaca ttcatcatat cgtcacggat 16440 ggcatgtcga tgggcatctt cgtcgaagag ctgctgcgcc tgtataacgg tgagactctg 16500 gagccgcttc ggattcaata caaggaattc gccgcttggc agcagtcgga acctgtaaaa 16560 gagcggctga aacgtcagga agcctactgg ctggacgtgc tggaaggcga actgccgacg 16620 cttgaactgc ctacggactt tgtcagacct gccgctcgca gctttgaggg agatgtgctg 16680 cccttcagca tcgacaagca gatgaccgac agcttgcagc gcatcgccga taagaacggt 16740 gctacccttt atatggtgtt attggcggcc tattcaatcc tgctcagcaa gtactcggga 16800 caagaagact tcattgtagg catgccggtt tcgggccgca cacatgccga cctggagccg 16860 ctcatcggaa tgtttgtcaa cactttggcg attcgccatt atccgtccgg ggagaaggcg 16920 ttcctcgctt acttgaacga agtcaaagaa acgatgctgg gggcctacga ccaccaggat 16980 tatccgttcg aggagcttgt gaaaaagctg caggcgccgc gagatcaaag ccgcaatcct 17040 gtattcgatg tcatgtttgc tctggaaacc aaggaagata acgttcaaag cttcggggat 17100 atcaagatcg aatcttatcc ggaaactcat acggtttccc aatttgatct taccttggtc 17160 atttcgttgc tggatgaggg aatgaacggg cagtttgaat atgccaccaa gttgtttacg 17220 cgcaatctga tcgacaattt cgctcaggac ctgctcatga tcatcaccca aatttgcgaa 17280 cagccttcgg tgctgttgaa ggatatttcc ctgaacgggc aatccgaaca ggagcaagat 17340 gtgctagagg ccattgatat tattttctaa 17370 <210> 15 <211> 1836 <212> DNA <213> Paenibacillus polymyxa ATCC21830 <400> 15 gtgtactgca tggaagctga ccgacagccg tctgttcaag aacaaggcac agcggcgcct 60 ttcaagcgcc aaaccgctta cgccgcctgg aaagcgttcc gctggctgat gtcctatgta 120 agccgtcaca aaggctggat gatcgtcggt gtcttgtccg cgattgccgc cgccgttatt 180 gagatatgga cgggaagttt gatcgagcag ctgaccaccc aggccgagaa gggggcgggg 240 ccaatcgttc tgcaaatcgt gtatgcggtc tttgtggtca tcttgatcgg tgtgccggcg 300 aagtttttca tgagcttcgg cgtggagcga agcagtgcct ctgcggtgca ggatatccgc 360 aaccatgtca tgcgtcatat cggtaaactc ccggtctcct atttagaaaa gcagcactcc 420 ggcgacgtat tgtcgcggat caacaacgac ctgcagctca tccagcagtt tatgattcgg 480 gacctcgccc agtggtttta tcatccgctg ttgttcatcg gctgtttcgc ttatttgctc 540 tacctccaat gggagctgat gttgtacagc ctgctgttat ttcccgtagc gctgctgatt 600 tcccaatgga tcggcaagca gttggagcgg ttgacggagg aagcccaggc gaacatgggc 660 cgaatgaacg tcaacctcca ggatacgctt gggggtatgc ctattgtaaa aagctacctg 720 ctatccggca tgttatctcg ctcctaccaa gtgctgctgc aattgacggc ccaaaaaaag 780 ctggccgtga aaaagcggga agcctgggtc aacccgctgc tttccacgtt gatgatcagc 840 ccgatcattt tcgccgtcag ttacggaagc tatttgatct acaaagggca gctaggcgcg 900 ggagagctga tcgccttcct gtacttgctg aatctgtgtc tggaaccgct ggagcatatt 960 cccgagctca tcacgcggac gttcgaaatg accggtgccc tgagaagggt ctccgaaatc 1020 gtcgagcagc cgaccgaaat ggaaaatggt cgttcgcttc cgaaaacgag cgccgccccc 1080 atcaagtttc agaacgtaac cttcgggtat gaggagagat ccccgatcct gcggaatgtt 1140 agcttctcgg tgccggaagg gaagacgatc gcgcttgtcg gagcgagcgg cggagggaag 1200 agcacggtgt ttaagcttgt atgcggcttt tatccgcttc cgaaggacca gggcgagatc 1260 cgcgtgttcg gcagcctgat ccacggcgcc gatccggagc agcttcggtc gcatttttct 1320 gtggtaaccc aggattcata tttgtttagc ggcacgatcg ctgaaaatat cggctacggg 1380 cgggaagtag cgtcgatgga cgagattatc gaagccgcca aagccgctca ggcgcattcc 1440 ttcattatgc agcttgaggg cggctaccag acgtatgtcg gagagcgcgg aggctttttg 1500 tccggcgggc agcgccagcg cattgcaatg gcccgggctt ttctgaagga tgctcccgtt 1560 ctgctgctgg acgagccaac gtcggctctt gatccggagt cggaaagcgc ggttcaggaa 1620 gcgctgggcg tattgatgaa gcagagaacg acgatgatta ttgcccaccg gctttctaca 1680 gtacaaaacg ccgacgaaat ttgggtcatg gaacagggga gtattgttga aaagggcact 1740 catgaacaat tgctgaagat gaagggactg tacgcccagt cgtactatca ggaatttacc 1800 gagtctgccg aacgcaggga ggtggcgtac acatga 1836 <210> 16 <211> 1734 <212> DNA <213> Paenibacillus polymyxa ATCC21830 <400> 16 atgaaaaagg gcggatggct ctcgcaagtg aaagaactaa gctacttgct gatgtttatg 60 aaccgcaagc gcaaaaccca gtacgccatt ggcctggcag tgacggcgct cacccagaca 120 ttgttcctga tcgccttcag cttggtcgta cataacttgg ttgatttcgc cgtgttccga 180 gatacgtccc tgatagtgga agcctttatt attttgggcg cggccttgtt cctggaaaat 240 gtgatttctc cctggttcat ttacttatac cagcgcagtg tcgagctgac tgtgctgaat 300 atccgcaaac gtctttatga aaagctgtgc cgggtgcggc cgagattcct ggagcagacg 360 caccatgggg acttgctgtc gcgggtgaac aatgacgtaa cgaccgttga gtttacattc 420 tcgcaggttt actttgtttt gctgcttcaa gttgtctttt gcatcggctc cattgtctcc 480 atggtgttga tcgattggcg gtttgccggt gtatccttcg tcattctgct gctgtcctcc 540 gtggtcagcc tgaaatttgc gcgggatatt cgcgccttgt ccgaacaagg cttgcaaacg 600 ctcggtaaaa tgaccgaaaa attcaaagat tttatgggcg gcattcaaat tgtgaagctg 660 ttccgcatcc gcacgattta cggccagtac gaggcgttga acgaacaaat gacgcagacg 720 cttcggcaaa ccgcgcagaa gaacggcatg caggctgcgg tgaatcattt tatcagctac 780 gtcacgttct gcggcatcat cgtcatcggc agtcttcttt atgcctacgg actgatggga 840 atgggaagcg tcgccgccct ggcggttttg caagtgaatc tgacgcacgc cttgttgaac 900 ttggggacgg ttctgtcgat gacccaaaat tcgctcgcgg gcgctcaccg gattcaagaa 960 gtactaagag aggaagagga accggagcgt ttgggatctc ctcacagcga gctcgtgtcg 1020 gaggctgcgg tggagtttcg cgatgtggaa ttttcctatc aggcggataa aaaggtgctt 1080 gtcgacatct ccatgcaggt gtttcccgga caggtcgccg ctatcgtggg ggccagcggc 1140 agcggcaaaa gtacgctgat caagctgctg ctcggctttt atcctgtgga cagcggagaa 1200 atcctgctcc aaggcaaacc gttcggccat tacacgctgg acgaaatccg caggcagatt 1260 gcatacgttc cgcaggagcc gttcttgttt accggcacaa ttgaggaaaa catccgctac 1320 ggcaacctgg atgcaacgga tgaagaagtg atcgaagcgg ccaaagcggc gtacgctcac 1380 catttcattc aggaacttcc tgaacagtat aaaacgccgg tgggagagag aggagcttcg 1440 ttgtcgggcg gacaaaggca gcgaattgcg atcgcacggg ctattctcaa aaatgccccg 1500 attctgctgc tggacgaagc gacttccgcg ctggataacg aatcccagca ttgggtacag 1560 caggccctga acgtattgat gaaagggcgc accaccatcc tgatcgccca ccgtctcagc 1620 accgtggaac atgcggattt gattaccgtc atgaaccaag ggacggtcgt cgagcggggc 1680 tgccatcagg acctgattgc gctcggaggt tattacgccc ggctgtacgg ctaa 1734 <210> 17 <211> 1365 <212> DNA <213> Paenibacillus polymyxa F4 <400> 17 tagcggcgga cgggtgagta acacgtaggc aacctgccca caagacaggg ataactaccg 60 gaaacggtag ctaatacccg atacatcctt ttcctgcatg ggagaaggag gaaagacgga 120 gcaatctgtc acttgtggat gggcctgcgg cgcattagct agttggtggg gtaaaggcct 180 accaaggcga cgatgcgtag ccgacctgag agggtgatcg gccacactgg gactgagaca 240 cggcccagac tcctacggga ggcagcagta gggaatcttc cgcaatgggc gaaagcctga 300 cggagcaacg ccgcgtgagt gatgaaggtt ttcggatcgt aaagctctgt tgccagggaa 360 gaacgtcttg tagagtaact gctacaagag tgacggtacc tgagaagaaa gccccggcta 420 actacgtgcc agcagccgcg gtaatacgta gggggcaagc gttgtccgga attattgggc 480 gtaaagcgcg cgcaggcggc tctttaagtc tggtgtttaa tcccgaggct caacttcggg 540 tcgcactgga aactggggag cttgagtgca gaagaggaga gtggaattcc acgtgtagcg 600 gtgaaatgcg tagagatgtg gaggaacacc agtggcgaag gcgactctct gggctgtaac 660 tgacgctgag gcgcgaaagc gtggggagca aacaggatta gataccctgg tagtccacgc 720 cgtaaacgat gaatgctagg tgttaggggt ttcgataccc ttggtgccga agttaacaca 780 ttaagcattc cgcctgggga gtacggtcgc aagactgaaa ctcaaaggaa ttgacgggga 840 cccgcacaag cagtggagta tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt 900 cttgacatcc ctctgaccgg tctagagata gacctttcct tcgggacaga ggagacaggt 960 ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc 1020 aacccttatg cttagttgcc agcaggtcaa gctgggcact ctaagcagac tgccggtgac 1080 aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc tgggctacac 1140 acgtactaca atggccggta caacgggaag cgaaatcgcg aggtggagcc aatcctagaa 1200 aagccggtct cagttcggat tgtaggctgc aactcgccta catgaagtcg gaattgctag 1260 taatcgcgga tcagcatgcc gcggtgaata cgttcccggg tcttgtacac accgcccgtc 1320 acaccacgag agtttacaac acccgaagtc ggtggggtaa cccgc 1365 <210> 18 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> pmxCC primer <400> 18 gagcttttct gaaggatg 18 <210> 19 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> pmxDN primer <400> 19 ggtcgttacg tcgttgttc 19 <210> 20 <211> 40965 <212> DNA <213> Paenibacillus polymyxa F4 <400> 20 atggcttttg aaaaagaaac gttgttttgg aacgaaaaat tcggtagcga cgattatacc 60 ttgacgcggc tgccttacag caaagctcca agctccctgg cgcccattat gacaaccgtc 120 ggcgggacgc tttcggagga agtggcgcag cgcgtccttc aaatgagcaa gggcgctccg 180 ctggccactt ttatgatttt gctcgctggt gttcagtcgc tcttgcataa atatacaggc 240 gcttctgaca ttctggtcgg catgccggtt gtacggaaac cgacggagac gcgccgatcc 300 gttaatcata cgatcatttt gaaaaactcg ctttcggcgg gtgcgacttt taaaacgctt 360 ctgagcgagc tgaggacttc cttgccggaa gcgattcagc atcaacatat tccgttcttg 420 aaaatgacgg agaagctgga cctgcaatat gcaggcggga tacccgtcgt ccatacgcta 480 gtatccctta aggagctgca tctggacgaa atcgggcaaa acgtggtcac agattgttcc 540 tttgaattca gcttaaccgg cgggacgata cagctagcgc tgtcatataa cgagcactta 600 tatgactccg agttcatgac tcgggtcgtc ggccatctga atcgcctgct ggccgtgggg 660 cttcacgagt tggagctgga catcgtgcgg gcggacatgc tgtcggagga cgagaaattt 720 caattgctgc aaagctttaa cgataccgag aaggactatc cccgtgatcg gacgattcat 780 cagctcgtgg aggagcaggc gaagcgggtg cccgaagcga cggcggttgt ctttgagggg 840 cggcggcttt cgtacgcgga gctgaacgaa cgggcgaacc ggctggcgcg gacgctgcga 900 tcggtcggcg tgctgcccaa tcagctggta ggcatgatgg ccaggagatc gctggagacg 960 gtcgttggca ttctagcggt tttgaaagct ggcggtgcct acgtgccgat cgacccggaa 1020 tacccggaag aacgcatccg ctacattctg gagaactcga acgcgcagct gctgctgact 1080 caaagggagt tgctgcagca ggtgccgttc gaagggaccg tggtggcgct ggatgacgag 1140 caggcctaca gcgacgatgg ctcgaacctg gagccggcca gcggtccgaa cgatctggct 1200 tatgtcatct atacatcagg tacgacgggc aaacccaaag gggtcatgct ggagcatcgc 1260 gggctggtca gcctgaaact gacgtttgcc catacgcttc acacgaccga acaggatcga 1320 gtgctgcaat tcgccagcct atcgttcgat gcgtcgtgct gggagatgtt caacgctctg 1380 tattttgggg cgaccttata tataccgtca accgagacga ttttggatga tcagctgttc 1440 gaacgtttca tgaacgagca tgcaattacg atcgccactt tgccgccaac ttatgcggct 1500 tatttgaatt cagaccgcct gccgagtctg agcaggctca tcacggcggg ctccgccgtt 1560 tcagccgaat tcgtgcagca gtggaaagac aaggtacagt actataatgc gtacggtccg 1620 acggaagcct cgatcgccac atccgtgtgg gcggcgtcta cgcatgacac cgaacgcaga 1680 gcgataccga tcggacgtcc gatctggaac catcgcttgt atatactggg cgcacagaat 1740 cagctggcgc ctatcggagt ggagggagaa ttatgcatcg cgggcgtcgg cctcgcccgg 1800 ggatattgga accggccgga tctgacggcg gagaaattca tcgaacaccc gtttgagccc 1860 ggagagagac tgtaccggac gggcgacttg gcccgctggc tgccggacgg aaatattgag 1920 tacttgggcc ggattgacca tcaggtgaaa atccgtggat tccggatcga gatcggcgag 1980 attgaagagc agcttctgaa gatcgactcc gtgcaggaga cgatcgtaat cgcgcgggaa 2040 ggcaaaagcg ggcaagaact gtgcgcttac ctggtcgcgg ggcgcccgct tacgctcggc 2100 gagctaagaa gcgcgctggc gcaaaaattg ccgaattaca tgattccggc gcattttgtt 2160 cagcttccgc agatgccgct cacgccgaac gacaaaatcg accgcaaggc tttgcccgct 2220 ccggaaggaa acgcgctgac cggcggcgcg tacgtagctc cccgcaatga agccgagcgg 2280 acgcttgccg atgtgtggca ggcggtattg aacgccgatc gcgttggggt aacggatcat 2340 ttcttcgagc tgggcggaga ctcgatcaag tccattcaag tatcttcgcg gcttcatcaa 2400 gccgggtaca agctggaaat ccgggatttg ttcaaatatc cgactatttc acagctcagc 2460 ctgcatgtga aaccgatcgg acgtaccatt gatcaaggcg aaataacggg tgaaacggcg 2520 ctgatgccga ttcagcattg gtttttcgag agctcctttg cggacccgca tcatttcaac 2580 cagtcggtga tgctgtaccg gaaggaacgc ttcgacgaag agacggtgcg tcaggtgctg 2640 caaaagctgg ccgagcatca tgacgccttg cggatggtgt tccgcaagac ggaacaaggg 2700 tttagcgcgc ggaaccgcgc gattcaggaa ggcgggctgt tcacgctgga cgtgttcgac 2760 ttcaaggatg cggagaatac cgcgcaggct gtggaagcga aggcaacgga cattcaagcg 2820 ggcatcgatc tggagaacgg gcccctcgcg aaggcgggac tgttccggtg cgcggacggc 2880 gatcatttgc tgctcgcggt tcatcatgcc gtggtggacg gcgtgtcttg gcgcattttg 2940 atggaggatt tcgctctggg ttacgagcag gccggcaaaa gcgaggaaat tcgtttcccg 3000 gcgaaaacgg atgcgtaccg cacttggtcc gagcagttgg ccgcttacgc gcaaagcccg 3060 gagatggcga aggaacgggc ctattggcag gccgtggaac aaattgcggt tccggccgtg 3120 ccgaaggatc tggaggcgga cgttacgacg cagcaggaca gcgaatcgct gttcgtccgt 3180 ttgactcccg aagaaacgga gctgctgctg aagcgggttc accgggccta caacaccgaa 3240 atgaacgata ttttggtaac ggcgctcggc atagccgttc gcaagtggac gggacacgaa 3300 cgggtgcgga tcaatctcga aggacacgga cgcgaatcga tcggaacgga tatcgacatc 3360 acgcgcacag tcggctggtt tacgaccaag tttccggtcg tcctggagcc ggaaaccgac 3420 cgggatttgg cctatcagat taaacaggtc aaggaaagct tgcgccgcat tccgaacaag 3480 gggcttgggt acggcgtatg ccgctatctc tccaaatcgg aggatggctt tgtttggggc 3540 gcagagccgg aaattaattt taactacctc ggccagttcg gcgatgatgt caatcaggac 3600 gagatcggca tatcttctta ttccagcggc agcccggcca gcgaccggca ggcccgcagc 3660 tttgtgctgg atatcaacgg catggtgctg gacggcgctt tatcgctcga tctcagctac 3720 agccggaagc agtatcgcaa ggaaacgatg gaagccttcg ctcagcggct tgagcaaagt 3780 ctccgagagc tcattaccca ctgcgcaggc aaagaaaaca ccgaattgac gccgagcgac 3840 gtgcaattta aaggcttgac catcgcggaa ttggagcaaa tcgcccagcg ctcgggccat 3900 ctcggggaaa tcgaaaatat ttactcgctt acgccgatgc agaagggcat gtggttccac 3960 agcgcgcttg accggcaaac ggccgcttac ttcgagcaga cgcggtttac gatgcgggga 4020 gcgctcgacg tccagctttt cgagaggagc tggatggagc ttgcgaaacg tcatctggtg 4080 ctgcgggcga attttgtgaa aggaccggcg ggcgagccgc tgcaaatcat ataccgcgac 4140 aagccggtcg gctttgaata tgaagagctg cttcatttgc aggcggacga gaaacaagcg 4200 tatttggata aaaaggcgga ggatgacaag cttcgcggct tcgacatgga acatgacgcg 4260 ctcgttcggg ttacgatcct acgcaccgaa gagcaaagct atcatgtgct gtggagtttc 4320 cagcatattt tgatggacgg ctggtgcctg ccgcagctga cgcaggagct gtttgagacg 4380 tactcggcct tggcatccgg caagcagcca gcgggagata agggatcgga ttatggcgct 4440 tatatcgaat ggttggagaa acaggacgat caggcggcat ccggctattg gacggcattc 4500 ctggcaggtt atgaagggca aaccgtactc ccgggacaaa aggaaccggc gccaaacggc 4560 agatttacgg ctgatcacgt caccgccgag ctgggcaagg acttgagcga gcggatggac 4620 cgggtggcga aacagcgcct ggttacggtc aatacgctgc tgcaagccgc ttggggcgtg 4680 atgctgcaaa aatataacgg aacaaacgat gccgtattcg gcagcgtcgt ggccggaaga 4740 ccggcggaaa tcccgggcat cgagtccatg atcgggctgt tcatcaatac ggtgccggtc 4800 cgcgtcacga gcgaagcgga caccgtgttc gccgacctga tggcgaagct ccaagagcgg 4860 gcgctggagt ccgggcgtta tgattactat ccgctgtatg aaattcaagc ccgcagcgtg 4920 caaaagcaga acctgatcaa ccatatcatc gctttcgaga actatccggt ggacgagcag 4980 atggagcagg cgggcgacca gcagcacggc gacctgacga tcgctgacgt tcagatggag 5040 gagcagacga actataactt caacgtgacc gtggtgccgg gagtcgagat cgaaattcga 5100 ttcgacttta acgccgaagt gttcgataaa gacagcatcg agcggctcaa ggggcatctc 5160 gtccatctgc tggagcaggt gacggataac ccggaaatta ccgtgggcga gctggaactt 5220 gtgacggagg cggaaaaggc cgaccttctc ggacgtttta acgacaccac cacggaattt 5280 ccgcgcggga agacgctcat tcaattgttc gaagagcagg cggagcgcat cccggatgca 5340 gccgctatct ccttgaatga gcaagagctg acctaccgcg agctgaacga acgcgtcaac 5400 cgccttgccc gtaccttgcg tagccacggg atatccaaag gccgtctggt cgccattatg 5460 gctgagcgtt ccatcgaaat ggtggtgggc atgctggcgg cacacaaagc cggagcggct 5520 tacgtaccga ttgacccgga atatcccgag gagcgtatcc gtttcttgat tgaggattcg 5580 ggagcgcagg tcatgctgac gcaaagccgc ttgcgcgagc gcctggcggg ttcggactcc 5640 gtgatcttac tggatgacga gtccttctat cacgaggacg gcacaaatct aaatccgggc 5700 accgaagcga cggatctggc ctgcgtcatc tatacgtcag gcacgacggg caagccgaaa 5760 ggcaaccttg tttcgcaccg caacatcgtg cggatcgtgc gaaatacgaa ttatatcgac 5820 atcaccgaac gggatcatgt cctccagctt tcgagctatt cgttcgacgg agcgattttc 5880 gatattttcg gcgctttgac caacggggcg cggctggtgc tggttccccg cgagactttg 5940 ctggaaatcg tccggctggc ggatctcatc cagcgtgagc gcatctcggt catgctgatt 6000 acgacggctt tcttcaacgt ccttgtagat gtgaacgtcg actgcctgcg ggatgtccgg 6060 gcgattttgt tcggaggaga gcgagtgtcg gtcggacatg tgcgtaaagc gctcgcccat 6120 atcggaccgg gcaggctcaa ccacctgtac ggcccgtcgg aaagcacggt ttataccacg 6180 taccttccgg tcgacttcgt cgatgagtcg gcggttactg tacccatcgg acggccgatc 6240 agtaatacga aggtgtatat cgtcgacagc cggaataagc ttctgccgat cggcgtggcc 6300 ggggagcttt gcgtcggcgg agaaggcttg gtacggggct acaataaccg gccggagctg 6360 acggcggaga aatttgtgga caatccgttt gtgctgggag agcgcatgta ccggacgggg 6420 gatttggcga aatggctgcc ggacggcacg atcgaatacg tgggacggac ggacgaccaa 6480 gtgaaaatcc gcggcttccg tattgagctg ggcgagatcg aagctcagct tcagaaagtg 6540 gagggaattc ggaaagcgac ggtattcgcg agggaagacg cctccggcga gaagcagctt 6600 tgcgcttatt atgaagcgga ccgcgagctt ccggcggccg agctgaagag cgtgctttcc 6660 caggaactgc cggcctatat gattccggcg tacctgatcc agttggagca gctcccgctg 6720 acgacgaacg gcaaggtcga ccgccgatca ctcccggcgc cggaggagag cttgcagccg 6780 ggcgaagaac gtactccgcc tcggactccg ctggaagcca gcttggcggg aatttggaaa 6840 agcgtgctcg gactggagca catcggggtt catgacaact tcttcgacct gggcggccat 6900 tccctgcggg cgacgacact ggtgagcaag gtgcatcagg agctgaacgt cgagctgcct 6960 ctgcgcgacg tattccgcta ctcgacgatc gaagagatgg ctctcgccat ctcccggatc 7020 ggagagcagt cgttctcgtc gattccgctg gcgggcgcaa gagcatatta tccgctttcc 7080 tcagctcaga agcggctgtt tatcctgaat cagctggaag gggccgatca gagctacaac 7140 atgccgggcg tgctgctgct ggaaggatcg attgaccgga gcctgctgga gaaggctttc 7200 cgcggactga tcgcacggca cgaaacgctg cgaaccggct ttgagatcgt acaaggcgaa 7260 gctgtacagc gcatttacga gagcgtcgac tttgccgtcg ggtaccgtca tgtgagcgag 7320 gaagaagcgc ccgaagtcgt gcaggccttc atccggcctt tcgacttggc gaagcctccg 7380 ctgctgcggg cggagctcgt agagctggca gccgaacgtt atttgctgat gttcgacatg 7440 caccatatcg tctccgacgg ggtttcgatg gacgtgttag tcgaggaact cgttcgtctg 7500 tacggcggcg agtcattaga gcctttgcgc attcaataca aggactatgc ggtatggcag 7560 cagtcggacg agcaaaaagt gcagttgaaa cgcgaggaag cttactggct ggaccgttac 7620 cggggcgagc tgccggttct ggaaatgccg acggactatc cgcgtcctgc cgtgcagagc 7680 tttgagggac aaacgctgac gtccttcgtg gacgaggcaa cgaacgaagg cttgaagcag 7740 ctggccgctc aaagaggaac gacgctgtat atggtgctgc ttgcggcata taccgtgctt 7800 ttgcataaat acacaggtca ggacgatttg atcgtcggaa cgtcgattgc gggtagaacg 7860 cacggagaaa cgcagccttt gatcggaatg ttcgtcaata cgctggcgct ccgcaattat 7920 ccggcttcgg agaagacctt cctgtcgtat ctggaagaag tgaaagaaac gaccttaggc 7980 gcttacgagc atcagaatta tccgttcgaa gagctcgtcg ataaagtgca ggtcagccgg 8040 gatttgagcc gcaacccgct gtttgacacg atgttctccc tgcaaaactt ggaggataaa 8100 gagtttgagc tggaagggct gaaattgtcc ccgtacccta gcgaatacgg cacggccaag 8160 ttcgacctga gtgtggatgt tacggaagaa aacggcggcc tggagtgcag ctttgaattc 8220 gcaacggctc tttataaaga aagcacgatc cggcggctgt cgactcattt cggacatttg 8280 ctcgtaacga tcgtaagccg tccggatgcg aagatcgccg agctgaactt gctgacggca 8340 gaggaaaaag aacaaattct cggcgcgttc aacccggcgc agccggaagc ggctcctgcg 8400 tcggcgttcc accggctgtt cgaggaacag gcggagcgca cgccggaagc ggaggccgtc 8460 gtgtacgaga acgaccggct tacgtatgcg gagctgaacg agcgggcgaa ccgcttggcg 8520 gctacgctgc gtgcaagcgg catcggccgg gaggcgatcg tcggcattct cgccgagcgt 8580 tcggtggact tgctggtggc cgtgctggcc gtctggaaag tgggcggggc gtatgtgccg 8640 ctcgacccgg attacccggc ggagcgcgtg cggttcatgc tcgaagacag cggagcgaag 8700 gtgctgctga cgcaaacgcc gctgcgagag cgtgccgaag catggctcgg cgaagaggag 8760 ctggcgctgg cggcggtgct gtacctcgac gacgaagcgt cgtacagcga cgagcgggcg 8820 aatgcgtcgg ttgtctccgg caagctgacg gatgctgtag atgacggcga tgagagccat 8880 cagaatgttg gcaccgacag cttccatgaa gcccgtccgg aggatctggc gtacgtgatc 8940 tatacgtcgg gaacgacggg caagccgaag ggcgtgatga tcgagcaccg cagcctggtg 9000 aatacggcgg cgggctaccg gcgggaatac cggttggatc agttcgcggt gcggctgctg 9060 cagctcgcca gcttctcgtt cgacgtgttc gtgggagata tcgcccggac gctgtacaac 9120 ggaggcacga tggtgattgt gccgaaggac gaccggatcg atccgtctcg tctgcaccac 9180 tggattgagc gggagcgggt caccatcttc gaatcgacgc cggcgctgat cgtgccgttc 9240 ctagagtacg tgtacgagca gcaactggat atgagccggt tggagctgtt gatcacgagc 9300 tcggacagct gcagcgtggc ggattaccgg accttgcagg aacgcttcgg ctcgttgttc 9360 cggatcatca acgcatacgg cgtgacggaa gcggcgatcg actcaagctt ctacgacgag 9420 gagctggcga agctgccgca gacaggcaat gtgccgatcg gcaaagcgtg gctgaatgcg 9480 aaattctaca tcgtggatgc gcatctgaac ccggtgccgg tcggggtgct gggcgagctg 9540 gtcatcggcg gagtcggagt ggcgcgcggg tacttgaacc gtccggagct gacagaagag 9600 aagttcgtag acagcccgtt cgccgagggc gagcggctgt accgcacggg agacttggcg 9660 cggtggatgg aagacggcaa cgtggacttc atcggccgga tcgacaacca ggcgaaaatc 9720 cggggctacc ggatcgagac gggcgagatc gagtcgcagc tgctgcgggt ggaaggcgtg 9780 cgcgaagcgg tggtgctggt tcgaagtgac gcgaacgggc agaaggcgct atgcgcgtat 9840 tacacgccgg ataccggagc ggaactggcg gtgaacgatc tgcgcggcgc gctggcgcag 9900 gagctgccgg gctacatgat cccgtcgtac ttcgtggagc tggagcgcct gcctctgacg 9960 ccgaacggaa agattgaccg gaaggcgctg ccagcgccgg aaggggaagc gggaagcgga 10020 acggagtacg tcgcaccgcg caatgagctg gaaacgaagc tggcggcgat ttggcaggag 10080 gtgctggggc ttgcgaagga gattggcgtt cacgacaact tcttcgacat cggcggccac 10140 tccctgcggg cgacgacgct ggtcagcaag attcataaag agttgaacgt ggatctgccg 10200 ctgcgcgacg tgttccgcca ttccacgatc gagagcatgg cggccgccat ttcccggctg 10260 gatgagcaaa cattcgttgc cattccggtg gcggatgacc gagaggtgta cccgcaatct 10320 tttgctcaaa aacgtctctt tatcctgaat caactggaag gcgcggagct tagctacaac 10380 atgccggaag cgatgctgct ggagggtgct ttagaccggg caaggttcga agaagcgttc 10440 cgtaagctcg tggcgcggca tgaaacactg cgcaccgggt tcgaaatggt ggatggcgaa 10500 gcatcgcagc ggatttacca ggacgtgaat tttgccgtgg agttctatcg agcggatgag 10560 caagaggccg aagagacggt tcgccgtttc gtccgtccgt ttgacttggc gaagcctccg 10620 ctgctgaggg taggccttgt cgagctggct ccggaacgcc atattctaat gtacgacatg 10680 catcatatta tttccgacgg cgtctcgatg gaaatctttg ttgaagaatt cgtccgcttg 10740 tacggcggcg agcaattgga gcctctgcgc attcagtaca aagactacac cgtttggcag 10800 cattcgcagg agcagaagga acggcttcag cgtcaggagg cgtactggct ggacatgttc 10860 caaggcgagc ttccggtgct ggaaatgccg accgactatc cgcgtccggc cgtgcagagc 10920 tacgaaggcc aaacgctgga gtttttcttt gacgcttcga aaaccgacgg cctgaagcag 10980 ctggcctcgg aaacgggcac gacgctgttt atggtgctgc ttgcggcgta taacgtcctt 11040 ctgcataaat attcaggtca ggaagacgtg atcgtcggta cgccgattgc cggaaggaat 11100 catggagatg tgcagccgtt gatcgggatt ttcttaaaca cgctggctat ccgcagttat 11160 ccggcttcgg agaagacatt cctgtcatac ctgaacgaag tcaaagaaac gaccctccac 11220 gccttcgagc atcaaaacta tccgttcgaa gaattggtgg acaaggtgca agtcacccgt 11280 gatttaagcc gcaatccgct cttcgacacg ctgtttacga tgcagaatac ggagaacgag 11340 gaatttgagc tggaagggct tcgcctgatt ccttatccga gcgcactgga taccgcgaag 11400 tttgatatca gcttggatgt gggcgaggag aacggcggct tggattacag cttcgaatat 11460 gcgacggctc tctacaaaag ggagacgatc gaacggctgg caaaacatta cgagcagctg 11520 ctcgtaacga tcgtaagccg tccggatgcg aagatcgccg agctgaactt gctgacggca 11580 gaggaaaaag aacaaattct cggcgcgttc aacccggcgc agccggaagc ggctcctgcg 11640 gccgcgttcc accggctgtt cgaggaacag gcggagcgca cgccggaagc ggaggccgtc 11700 gtgtacgaga acgaccggct gacgtatgcg gagctgaacg agcgggcgaa ccgcttggcg 11760 gctacgctgc gtgcaagcgg catcggccgg gagacgatcg tcggcattct cgccgagcgt 11820 tcggtggact tgctggtggc cgtgctggcc gtctggaaag cgggcggggc gtatgtgccg 11880 ctcgacccgg attacccggc ggagcgcgtg cggttcatgc tcgaagacag cggagcgaag 11940 gtgctgctga cgcaaacgcc gctgcgagag cgtgccgaag cctggctcgg cgaagaggag 12000 ctggcactgg cggcggtgct gtacctggac gacgaagcgt cgtacagcga ggagcgagcg 12060 aatgcgtcgg ttgtctccgg caagctgacg gatgctgtgg acgacggcga tgagagccat 12120 cagaatgttg gcaccgacag cttccatgaa gcccgtccgg aggatctggc gtacgtgatc 12180 tatacgtcgg gaacgacggg caagccgaag ggcgtgatga tcgagcaccg cagcctggtg 12240 aacacggcgg cgggctaccg gcgggaatac cggttggatc agttcccggt gcggctgctg 12300 cagctcgcaa gcttctcgtt cgacgtgttc gtgggagata tcgcccggac gctgtataac 12360 ggaggcacga tggtgattgt gccgaaggac gaccggatcg atccgtctcg tctgcaccac 12420 tggattgagc gggagcgggt caccatcttc gaatcgacgc cggcgctgat cgtgccgttc 12480 ctggattacg tgcacgagca gcaactggac atgagctgga tggagctgtt gatcacgagc 12540 tcggacagct gcagcgtggc ggattaccgg accttgcagg aacgcttcgg ctcgttgttc 12600 cggatcatca acgcatacgg cgtgacggaa gcggcgatcg actcaagctt ctacgacgag 12660 ccgctgacga agctaccgca gacaggcaat gtgccgatcg gcaaagcgtg gctgaatgcg 12720 aaattctaca tcgtggatgc gcatctgaac ccggtgccgg tcggggtgct gggcgagctg 12780 gtcatcggcg gagtcggagt ggcgcgcggg tacttgaacc gtccggagct gacggaagag 12840 aagttcgtag acagcccgtt cgccgcgggc gaacggctgt accgcacagg agacttggcg 12900 cggtggatgg aggacggcaa cgtggacttc atcggccgga tcgacaacca gacgaaaatc 12960 cgggggtacc ggatcgagac gggcgagatc gagtcgcagc tgctgcgggt ggaaggcgtg 13020 cgcgaagcgg tggtgctggt tcgaagtgac gcgaacgggc agaaggcgct atgcgcgtat 13080 tacacgccgg ataccggagc ggagctggcg gtgaacgatt tgcgcagcgc gctggcgcag 13140 gagctgccgg gctacatgat cccgtcgcac ttcgtggaga cggaacgcct gcctctgacg 13200 ccgaacggaa agattgaccg gaaggcgctg ccagcgccgg aaggggaagc gggaagcgga 13260 acggagtacg tcgcaccgcg caatgagctg gaaacgaagc tggcggcgat ttggcaggag 13320 gtgctggggc ttgcgaagga gattggcgtt cacgacaact tcttcgacat cggcggtcac 13380 tccctgcgag cgacgacgct ggcgggcaag gtatttaagg aattaaacgt caacctgccg 13440 ctgcgcgacg tattccgtca ctcgacgatt gcggcgatgg ccgaggcgat cgcccggatg 13500 gaacggcggg agcatgaggc cattcctcaa gcggaggaga gagagtacta ccctctgtcc 13560 tccgcgcaga aacggctgtt cattcagcac acgctggatg gagcggatca gctttacaac 13620 atgccggaac tggtgcaggt ggaaggcgag tttgatttag accggttgga agccgccttg 13680 cggaaattga taacacggca tgaatcgctg cgcaccggtt ttgaaatcgt gaaggccgaa 13740 gcggttcagc ggatttaccc gcaggtcgat tttgctatcg agcatcatca agcggataaa 13800 gaggatgcgg ctcaaatcga gcagatcgtc cgcagcttcg ttcgtccgtt tgatctcggc 13860 aagccgccgc tgctgcgcgc cggggtcatc gagctggagc cgaacctgca tattctcctt 13920 ttcgacatgc accatatggt gtccgacggc gtatcgatgg cgattgtgat cgatgagttc 13980 tcgagtttct acgccgggga agaactgccg ccactgcgca ttcaatacaa ggattatgcc 14040 gtttggcagc agtcgaaggc ccaccgagag cggatcgggc ggcaggaagc gtactggctg 14100 caaaccttcg aaggcgagct gccgacggcg gacctgccga tggactacga acggtccgcg 14160 gctcgcagct acgaaggcgc gcatctggag ttcgacgtcg aagcttctct ctctgcgcgg 14220 ctgcgcgaat tggcggccga gcgtgaaagc acgctgttca tggtgctgct tgcggcttat 14280 accgtgctgc tgtccaagta cagcgggcag gaggacttgg tcgtgggcac cccggtggcg 14340 ggaagaacga acgccgattt ggaaccggtc atcgggatgt ttgtcaatac gctggcgatc 14400 cgcaatcgtc cgtcgggcga caaaacgttc ttgtcctacc tggaagaagt gaaggaaacg 14460 gctttgggtg ctttcgagaa ccaggattat ccattcgagg agctcgtgga gcgtttgaat 14520 gtgaagcggg agccgggccg cttcccgctg ttcgatgccg ttttcgactt gcaaaatatc 14580 gaagaacgag acgccgagct ggaaggggtc agcctgaaga cttacgagct tgaccatttg 14640 gaagaagcga agttcgatct gacgctgttt atgtatgaaa acaacggggc gctgagcggg 14700 ggcttcttct acgccaccaa gcttttcaaa gaagcgatga tccgcacctt gaccgaggat 14760 tacctgcggg tactgtctca aattgcggaa aatccacaac tcgagctaag ccggattgaa 14820 tgtcataaac cggcggcagg cgcaaagagt gccgtcgata cgatcgaatt cgcgttctaa 14880 tcctacaagc gcgcctccgc cgtcaagtga aagcggcgcg catcccaagg aagacggcga 14940 acaacgtcca agcccgtaaa cgcgcaggcc gaaagcaagc tttttcggac ctgcgccggg 15000 gcatgcggtc acttcatttt taggggaggt acaaatgaaa tctttgtttg aaaaggaaga 15060 acggtactgg agcggcaagt ttgacgccga tgacagcctg agcttccttc cctacagtca 15120 atcctccaaa ttatccgccg acggggaagc tgcggccgag ccgggcttgc ttcaccgtac 15180 tctgccgagc gaactctcgg agagaatcat ttgcctcgcc aacggttcgg atttggcttt 15240 gtacatgatt gttttggcag gagtaaaaag cctgctgttc aagtataccg ggcgggacca 15300 agtgctggtc ggcatgcctt cttatagcgc agaccccgac gggactccgc cgccgcatga 15360 catcttggtg atcaagacgt ccgtaagccg ccagactacg ctgaaaacgc tgctcggggg 15420 catcaaagcc tccatcggcg aggcgctgga gcatcagcac ctgccttttc ggaaaatggt 15480 ggagccgctc catctggact atacggggga cggcctcccg gtcgtcaaca ccgtcgtatc 15540 cttcgccccg attcatccgg aaccgctggg taaccgggtg gcggccgata cggtttttcg 15600 cttcgaccgc caaaaccact ccatcgagct ggaaataagc tttgacgggc agcgttacga 15660 gcgggcattt gtggaacagg cggccgacca tcttgttagg ctgctgtccg tgcttttatt 15720 tcagccggat ctggagcttg gacaagccga tgtgctgtcc ccagacgaga gggagacgct 15780 gctgaagcga tttaatgaca ccgaaaccga gttcgagcgg gggaaaacga tttacggctt 15840 gttcgaagaa caggcggagc tttacccgga caacgtggcc gccgtcatga acgagcggca 15900 gctgacctac cgcgagctga acgagcgatc caaccgcctt gcgcggaagc tgcgagagac 15960 gggagtagaa gcggaccagc tggtagcgat tctggccgaa cgctcgctcg atatggttgt 16020 cggcattctg gcgattctca aagcgggcgg agcatacgtg cctgtcgatc ccgactaccc 16080 ggaggagcgc atccgcttca tgatcgagga ttcgggcgcg ccgttattgc tgattcaaaa 16140 gcatctgcac gagaagaccg acttcgcagg aacgcgcctt gaattggacg atttcgtgtg 16200 gggcgacaga ggggcggact ccgccgatgt gcgggacgct tcgaacctgg agccgatttc 16260 cgggccgggc aacctggctt atgtcatcta cacgtcggga acgaccggca gaccgaaagg 16320 aacgctgatc gagcataaga acgtcgtgcg cctcctgttc aacgacaaga acctgttcga 16380 cttcgggccg tccgacacgt ggacgctgtt ccactcgttc tgcttcgatt tctccgtctg 16440 ggaaatgtac ggagcgctgc tgtacggagg caagctggtc atcgtaccgc cgctcacggc 16500 gaaaaatccg gccgatttcc tggcgctgct gggccgcgaa caggtcacga ttttgaacca 16560 gacgccaacg tacttctacc agctgctgcg taaggtcttg gcggaccatc cgtacgatct 16620 gcggattcgc aacgtcatct tcgggggcga agcgctgagt ccgctgctgc tcaagggatt 16680 caagacgaag tacccggaga cgaagctgat caatatgtac ggcattaccg agacgacggt 16740 tcacgttacg tataaggaaa taacgtgggt cgaaatggag gcggcgaaga gcaatatcgg 16800 caagccgatc ccgacgctga gcgtgtacgt ccttgatgaa aaccgctccc ctgtgccgat 16860 cggtgtagcg ggcgaaatgt acgtggccgg ggaaggcctt gcgagaggat acctgaaccg 16920 tccggatctg acggcggaga agttcgtcga ttccccgttt gcggaggggg agaaactgta 16980 ccgctcgggc gacttggcgg cttggctgcc ggacggcaac atcgaatacc tgggccggat 17040 cgatcatcag gtgaaaatcc gtgggtaccg gatcgagctg gacgaaatcg agacgcagct 17100 gctgaagatc gccgccgtgc aagaagccaa ggtgctcgac cgcgacgacg cgaacggcca 17160 taagcagctt gtcgcttatt acgtcgcgga aacgaggctg gcggcgcatg aactcaagga 17220 ggagctcgcc aagcagcttc cggggtatat gattccttcg cacctcgtgc agctttcgcg 17280 gatgccgctg accccgaacg ggaaaatcga ccgcaaagcg ctgcccgcgc cggaggaagc 17340 cgcggccgga ggagcggaat atgtcgcgcc gagaacgctg ctcgaaatga agatcgcccg 17400 cgtctggcag gatacgcttg gcgttccgca ggtcggcgta aaggataact tttttgagtt 17460 gggtggcaat tcgttaagtc tgatgaggct cgttcaagcc gtttacgatg aaacggacat 17520 cgagataccg ctgaaccgcc aattccacaa tctaacagtt gaagccatgg ctttcggcga 17580 gggggatctg ggcctggata aagggggaga ctccttcatt aagctgaata aagcaggaga 17640 tctgaacgtg ttctgcttcc ctccgggcag cggcttcggc atcggttacc gagagctcgc 17700 aagcaggctc gacggccggt tcgtgctcta cggcattgat tttatcgacg atgccgtcga 17760 ttacgaggcc atgctgaacc gttatgtgga cgagatcgtc cgcatccagc cggaaggacc 17820 ttacgtgctg ctcggctact gcttcggagg caacctgacg ttcgaggtag ccaaaacgat 17880 ggagaaaaga gggtattccg taacggacgt gctcatgttg gactcgtgga ttaaggacac 17940 gctgacgcct tccgaaacgt cggagaaaga gcttgaagaa acgcttgccg atttcgacga 18000 agaagagaag gaattaatga gcaacccgct cgtgcgggag cgagttcatc ggaaggtcaa 18060 agcgaccttg gcgtacgaag cgcagcttat taattccggc acgatcccgg ccaggattta 18120 cgaactgatt gcgaaggaca gcgaagcgtt ccgcctggag caccaattgc cttcctggcg 18180 gggggcaacg acgcaagctt acgccgatta ccggctggag ggcgcgcacg aggaattgct 18240 ggaactcgcg cgcgtggaag aaacggccgt cgtcatccgg gacatcttag agcaagtcaa 18300 gcggcggatc gaagcggagg ccggggtact gcatggaagc tgaccgccag ccgtctgttc 18360 tagaacaagg cacagcggcg ccttccaagc gccaaaccgc ttacgccgcc tggaaagcgt 18420 tccgctggct gatgtcctat gtaagccgtc acaaaggctg gatgatcgtc ggtatcttgt 18480 ccgcgattgc cgccgccgtt attgagatat ggacgggaag tttgatcgag cagctgacca 18540 cccaggccga gaagggggcg gggccaatcg ttctgcaaat cgtgtacacg gtctttgtgg 18600 tcatcttgat cggtgtgccg gcgaagtttt tcatgagctt cggcgtggag cgaagcagcg 18660 cctctgcggt gcaggatatc cgcaaccatg tcatgcgtca tatcggcaaa ctcccggtct 18720 cctatttgga aaagcagcac tccggcgacg tattgtcgcg gatcaacaac gacctgcagc 18780 tcatccagca gtttatgatt cgggacctcg cccagtggtt ttatcatccg ctgttattca 18840 tcggctgttt cgcttatttg atctacctcc aatgggagct gatgctgtac agcctgctgt 18900 tatttcccgt agcgctgctg gtttcccaat ggatcggcaa gcagttggag cggttgacgg 18960 aggaagccca ggcgaacatg ggccgaatga acgtcaacct ccaggatacg cttgggggta 19020 tgcctattgt aaaaagctac ctgctatccg gcatgttatc tcgctcctac caagtgctgc 19080 tgcaattgac ggcccaaaaa aagctggccg tgaaaaagcg ggaagcctgg gtcaacccgc 19140 tgctttccac gctgatgatc agcccgatca ttttcgccgt cagttacgga agctatttga 19200 tctacaaagg gcagctaggc gcgggagagc tgatcgcctt cctgtacttg ctgaatctgt 19260 gtctggagcc gctggagcat attcccgagc tcatcacgcg gacgttcgaa atggccggtg 19320 ccctgagaag ggtctccgaa atcgtcgagc agccgaccga aacggaaaat ggccgttcgc 19380 ttccgaaagc gagcgccgcc cccatcgagt ttcagaacgt aaccttcggg tatgaggaga 19440 gctccccgat cctgcggaat gttagcttct cggtgccgga agggaagacg atcgcgcttg 19500 tcggagcgag cggcggaggg aagagcacgg tgtttaagct tgtatgcggc ttttatccgc 19560 ttccggagga ccagggcgag atccgcgtgt tcggcagcct gatccacggc gccgatccgg 19620 agcagcttcg gtcgcatttt tccgtggtaa cccaggattc atatttgttt agcggcacga 19680 tcgctgaaaa tatcggctac gggcgggaag tagcgtcgat ggacgagatt atcgaagccg 19740 ccaaagccgc tcaggcgcat tccttcatta tgcagcttcc cgacggctac cagacgtatg 19800 tcggagagcg cggaggcttt ttgtccggcg ggcagcgcca gcgcattgca atggcccggg 19860 cttttctgaa ggatgctccc gttctgctgc tggacgagcc aacgtcggct cttgatccgg 19920 agtcggaaag cgcggttcag gaagcgctgg gcgtattgat gaagcagaga acgacgatgg 19980 ttattgccca ccggctttct acagtacaaa acgccgacga aatttgggtc atggaacagg 20040 ggagtattgt tgaaaagggc actcatgagc aattgctgaa gatgaaggga ctgtacgccc 20100 agtcatacta tcaggaattt accgagtctg ccgaacgcag ggaggtggcg tacacatgaa 20160 aaagggcgga tggctctcgc aagtgaaaga actcggctac ttgctgatgt ttatgaaccg 20220 caagcgcaaa acccagtacg ccattggcct ggccgtgacg gcgctcaccc agacattgtt 20280 cctgatcgcc ttcagcttgg tcgtacataa cttggttgat ttcgccgtgt cccgagatac 20340 gtccctgatg gtggaagcct ttattatttt gggcgcggcc ttgttcctgg aaaatgtgat 20400 ttctccctgg ttcatttact tataccagcg cagtgtcgag ctgactgtgc tgaatatccg 20460 cgaacgtctt tatgacaagc tgtgccgggt gcgaccgaga ttcctggagc agacgcacca 20520 tggggacttg ctgtcgcggg tgaacaacga cgtaacgacc gttgagttta cattctcgca 20580 ggtttacttc gttttgctgc ttcaagttgt cttttgcatc ggctccattg tctccatggt 20640 gttgatcgat tggcggtttg ccggcgtatc cttcgtcatt ctgctgctgt cctccgtggt 20700 cagcctgaaa tttgcgcggg atattcgcgc cttgtccgaa caaggcttgc aaacgctcgg 20760 taaaatgacc gaaaaattca aagattttat gggcggcatt caaattgtga agctgttccg 20820 catccgcacg atttacggcc agtacgaggc gttgaacgaa caaatgacgc agacgcttcg 20880 gcaaaccgcg cagaagaacg gcacgcaggc tgcggtgaat cattttatca gctacgtcac 20940 gttctgcggc atcatcgtca tcggcagtct tctttatgcc tacggactga tgggaatggg 21000 aagcgtcgcc gccctggcgg ttctgcaagt gaatctgacg cacgccttgt tgaacttggg 21060 gatggttctg tcgatgaccc aaaattcgct cgcgggcgct caccggattc aagaggtact 21120 aagagaggaa gaggaaccgg agcgtttggg atctcctcac agcgagctcg tgtcggaggc 21180 tgcggtggag tttcgcgatg tggtattttc ctatcaggcg gataaaaagg tgcttatcga 21240 catctccatg caggtgtttc ccggccaggt cgccgctatc gtgggggcca gcggcagcgg 21300 caaaagtacg ctgatcaagc tgctgctcgg cttttatcct gtggacagcg gagaaatcct 21360 gctccaaggc aaaccgttcg gccattacac gctggacgaa atccgcaggc agattgcata 21420 cgttccgcag gagccgttct tgtttaccgg cacgattgag gaaaacatcc gctacggcaa 21480 cccggatgca acggatgaag aagtgatcga agcggccaaa gcggcgtacg ctcaccattt 21540 cattcaggaa cttcctgaac agtataaaac gccggtggga gagagaggag cgtcgttgtc 21600 gggcggacaa aggcagcgaa ttgcgatcgc ccgggcgatt ctcaaaaacg ccccgattct 21660 gctgctggac gaagcgactt ccgcgctgga taacgaatcc cagcattggg tacagcaggc 21720 cctgaacgaa ttgatgaagg ggcgcaccac cattctgatc gcccaccgtc tcagcaccgt 21780 ggaacatgcg gatttgatta ccgtcatgaa ccaagggacg gtcgtcgagc ggggccgcca 21840 tcaggacctg ctggcgctcg gggggtatta cgcccggctg tacggctaga cccgctttac 21900 ggcgggcggc ttcctgcttg cgaatgcgac agagtctttt agtaatccaa ggtatgactt 21960 tataaccggt aattaccaga aaacgtagac acgaaaaagg aggaagagtc cgaaggagcc 22020 gttccgggaa tggcctgcac ggttcttcta agagtgacta agtgacatct aggtacaaag 22080 cctatctata aagaatctct aaaccgggag tgtgtcgatg tgagagagaa taccaacgag 22140 caatatggat taacgcaagc ccagcgccga atatggttca tggaaattat gaatccggga 22200 acgtccatca cgatgctttc cgcgacctac cagattacgg gcgagatcaa cacacagctt 22260 ctggagcaag cggcggcaga gatcgtcaaa acctatgacg ttttccgaat ccgcattagc 22320 ggggatttgc aaaatccaac gcagtggttc gaagaaccgg agaatgtcca agctaggata 22380 agccgcctcg aaataggcac aaccgaacaa ttctatgctt gggtgaaaga agtaagcgaa 22440 aaaccggcca gcgtgttcga cgaacacctc taccaattta cgattatcca ttttgcgaac 22500 ggccaagtat ggctcaattt gacggtaaat catattatcg ccgacggctt gtccgtcaat 22560 gctttgctgc atgcggtgat ggaaaaatac ctggaactgc gcaaaggcat ctccagcagt 22620 taccaggccc cttcctatct ggattatatt tctgcggagc gtgaatatga gcaatcgcag 22680 cgttatcaaa aaggcaagga atactggctg acgaagtaca acactttgcc tgaaacgacc 22740 ggcattaaat cgtatccgcc attctcgatc ggcagcgaat ccaataaact ggccatcact 22800 ttggacggtt cccggtatga acgcattctg gccttcagcg aacaatatca ggtcagctta 22860 tatacgttat ttctgtccgc catgtacgcc ttattgtaca agctgaccga cagcaccgat 22920 gttccggtcg gcacggtttt cgccaatcgc accagcaaga aggaaaaaga aacgatcggc 22980 atgttcgtca gcaccgtggc tacgcggatc cgtctgaatc cagacaggca cgtgctttcc 23040 ttgatccaaa cggtttccaa ggaaaatacg gcggatctgc ggtatcagaa atacccttat 23100 aaccaattga tccaggattt acgcgaacaa cacggccgca acgatctttc ggggctgttc 23160 cgcacgtctc tggaatatct gcctttgaaa atcgtggagt acgaagaaat caaggtacgc 23220 ctggaggctc acttcgctag gcacgagatg gacgatttgc tgctgcgctt cgaccatatg 23280 ctgaatgaag gccatgtaat tctccatgct tcctatcgta ccggcctgtt cgagacggcc 23340 gagattgatc ggattatgga acagtatgta accgttctgg accagtttct tcagactccc 23400 gaactgccgg tacgcgagat ttctctgctg agcgatgagg agagacaccg cattctcaac 23460 gtttttaacc cgccggtggc agggctgagc gagggagaag cgtttcatcg gtatgttgaa 23520 aagtttgccc tggaaattcc tgatcatccg gcagtcgtct acatggacaa acagctgacc 23580 tacggcgaat tgaacgaacg cgccgagcgg ctggcttctc tccttcgcga acagggcgtg 23640 ggaagggaga cgattacggg gatctgggcg gagcgttcgg tggaactgct ggtcggggtg 23700 ctcgccgttt ggaaagccgg cggagcctat gtaccgctgg accccgatta tccggcggag 23760 cggattgagt acatgctcag cgatagcgaa gcaacggtgc tgcttacgca gcgtcatctg 23820 ttggagcggg ccggagtttg gttggccgat gaccggctga agcttcaagc tgtctatgcc 23880 atggacgatg aacagattta taacggggat gccttagccg tggaatttga atctgccggc 23940 agcgccccgc aagacttggc ttatgtgatt tacacctcgg gtacgacggg acgcccgaaa 24000 ggcgtcatga tcgagcacgg tagtctcgtg aatacggcgg atgcgtaccg tcgcgagtac 24060 cggttggatc agtttccggt gcggctgctg cagctggcca gcttctcgtt tgacgtgttc 24120 gtcggagaca tcgcgcggac gctgtataac ggaggcacga tggtgattgt gccgaaggat 24180 gaccggattg atccgaacct cttatacggc tggattcggg accaaaatat tacggtattc 24240 gaatcgacgc ctgcgctcat cctgccgttc atgcagcata tttatgaaga agggctggac 24300 gtcagctcca tacagttgct gattaccagc tcggatgctt gcagtgtcac cgattaccga 24360 ttgctgctgg aaagattcgg cggacaattc cgcatcatta acagctatgg cgttaccgaa 24420 gcggccattg acagcagctt ttacggtgag ccgctggata agctgccgcc gtcgggtcat 24480 gtgccgatcg gcaaagcttg gctgaacgcc cggttttaca ttgtcgatgc cgcgttaaag 24540 ccagttcctg taggggttcc gggcgagctt gtcataggcg gcgccggggt ggcgcgcggg 24600 tactggaacc gtctggacct aaccgccgag aagtttgtgg acagcccgtt tgtgccgggc 24660 gaacgtgtgt accggacagg cgatttggcc cgctggttgg aagacggcaa cgtcgacttc 24720 atcggccgga tcgactatca ggtgaaaatt cgcgggttcc gtatcgaact tggcgaaatt 24780 gaaacggccc tgctgcgttt cccgggcgtc aagcaggctg tggtgaccga ccgcacggat 24840 gagcaggggc aaaagtattt gtgcggctac gtggcggcgg atgcttcctt gcagctgagc 24900 gatctgctgt cccaattgaa gcaagagctg ccggcccata tggtcccggc ccggctggtg 24960 tctcttgata agcttccgct cacgccgaac ggcaaaattg accgtaaagc gctgcctgaa 25020 ccgaccggag aggtagaaac aggcggtgag tatgtggctc ctcgcacaac gctggaaaca 25080 agacttgctc tcatttggca gcaggtgctg ggtattgcgc gagttggagt ccaagacgat 25140 ttctttgact tgggtggtca ttccttgcgg gcctccacgc tggtttccaa gattcggaaa 25200 gagctgcaag tcgaggttcc gctgcgggaa gttttccgct acaccacgat cgaacagctg 25260 gcccaaagaa tcggcggttt aaggcagcag gagacgtatg agattacaaa ggcggctgag 25320 gccgagtact atccggtttc atccgagcaa aagcgtctgt acgtcctgcg ccagcttgac 25380 ggggccgagc gcagctacaa tatgtcggcg gcgcttcttc tcgaaggcaa gctggaccgc 25440 acgcgcgtcg agcacgcgtt ccgggcgctg attcagcgcc atgagacgct gcgtaccggg 25500 atcgagcagg ttcaaggcga gcttgtccag cgcatctatg acgaggtgga gtttgctgta 25560 gattatttcc aagcgagcga gtgggaagtg gaccaagtgg tggaagcata ctatcacccg 25620 tttgatctga ccaagccgcc acttctccgc atcggcctga tcgaagtcga cgaggatcgc 25680 cacattctgc tgttcgatat gcaccatatc gtctcggacg gcatttcgac agcgctgctc 25740 ttcgacgagt tcagccgcct gtatcggggc gaggagctgg ccccgctgcg cattcaatac 25800 aaagattatg ccgtttggca gcattccgaa gcttacgggc agatgctcca gccgcagaag 25860 gagtattggc tgaaacagct gtcgggcgag ctgccggtct tggagctgcc gacggacttc 25920 ccgcggcctg cggtgcaaag ctttgacggc cgaaccgtga agttttatat cgagaaagat 25980 cggacggaga agctgaaaga gctggcggca cggacaggga cgaccctgta catggtactg 26040 ctgtcggctt acaccatcct tatgcataaa tattcgggtc aggaagatct gatcgtagga 26100 acgccgattg ccggaagaac gcaggaagaa gtgcagccga tcgtagggat gtttatcaac 26160 acgctggcca ttcgcagccg tccggagcgt tccaagccgt acctttcgta cctggaagaa 26220 atcaaggaca tcacgctcgg ggctttcgaa caccaaaatt atttgttcga agacttggta 26280 gaaagtcttc acattccgcg cgcgaccggc cggaatccgc tctttgacac gttcttctcc 26340 ctacaaaata cggagaacga gcaaattgtc atcgaggggc tggagcaatc gttttatcca 26400 ctggaaaacc aaacatccaa gttcgagctg ctcctggata tttcggagct ggacggtcag 26460 ctcgaatgcc ggttggagta cgctacggct ttgtataaac aagagaccgc ggagcggttc 26520 gccagacatt atgacaagct gctcgaaacc atcgcagcag cgccggacgg ggatattgcc 26580 tcgctggaaa tgctcacgga ggaggaaatc cgcgagttgg tgcgcggttt caacgattcg 26640 gaggcggact acccgcggca gcagacgatt cacggcttgt ttgaagagca ggcggagctt 26700 tatccggaca acgtggccgc cgtcatgaac gagcggcagc tgacctaccg cgagctgaac 26760 gagcgatcca accgccttgc gcggaagctg cgagagacgg gagtagaagc ggaccagctg 26820 gtagcgattc tggccgaacg ctcgctcgat atggttgtcg gcattctggc gattctcaaa 26880 gcgggcggag catacgtgcc tgtcgatccc gactacccgg aggagcgcat ccgcttcatg 26940 atcgaggatt cgggcgcgcc gttattgctg attcaaaagc atctgcacga gaagaccgac 27000 ttcgcaggaa cgcgcctcga attggacgat ttcgttgggg gcgacagagg ggcgaactcc 27060 gccgatgtgc gggacgcttc gaacctggag ccgatttccg ggccgggcaa cctggcttat 27120 gtcatctaca cgtcgggaac gactggcaga ccgaaaggaa cgctgatcga gcataagaac 27180 gtcgtgcgcc tcctgttcaa cgacaagaac ctgttcgact tcgggccgtc cgacacgtgg 27240 acgctgttcc actcgttctg cttcgatttc tccgtctggg aaatgtacgg agcgctgctg 27300 tacggaggca agctggtcat cgtaccgccg ctcacggcga aaagtccagc cgatttcctg 27360 gcgctgctgg gccgcgaaca ggttaccatt ttgaaccaga caccaacgta cttctaccag 27420 ctgctgcgtg aggtcttgac ggatcatcca tacgatttgc ggattcgcaa tgtgatcttt 27480 gggggcgaag cgctgagtcc gctgctgctc aagggcttca agacgaagta cccggagacg 27540 aagctgatca atatgtacgg cattaccgag acgacggttc acgttacgta taaggaaata 27600 acgtgggtcg aaatggaggc ggcgaagagc aatatcggca agccgatccc gacgctgagc 27660 gtgtacgtcc ttgatgaaaa ccgccgccct gtgccgatcg gcgtagcggg cgaaatgtac 27720 gtggccgggg aaggccttgc gagaggatac ctgaaccgcc cggatctgac ggcggagaag 27780 ttcgtcgatt ccccgtttgc ggaaggggag aaactgtacc gctcgggcga cttggcggct 27840 tggctgccgg acggcaacat cgaatacctg ggccgaatcg atcaccaggt caaaatccgc 27900 gggtaccgga tcgagctgga cgaaatcgag acgcagctgc taaacgtcga gggcgtggaa 27960 gaagcggtgg tgcttgcccg tcaggacggc gggggcgaga aggcgcttgt cgcctacttt 28020 gtggcggacc ggacactgac ggtcagcgaa atgagaacct cgctggccca gggaatgccg 28080 gggtacatga tcccgtcgta cttcttgcag cttgagcgca tgccgctgac gtccaacggt 28 140 aaagtggacc gcaaagcgct gccggagccg caaggcggct tgcaaacggg cgtcgaatac 28200 gtagcgccgc gtaaccggac ggagtcccag cttgtgaaga tctgggagga agtgctgggt 28260 tactccggca ttggagtcct ggacaatttc ttcgagctcg gaggccactc cttgcgggcg 28320 acgaaccttg tcagcaagat tcggaaggaa atgaacgtcg aacttccact gcgcgatgtg 28380 ttccgctata cgacggtaga gtcgatggcc ggggctattg ccagcttgga agaaacacag 28440 cacagctcga ttccgaaagc ggaagagaaa gcgtactatc cggtttcctc cgcgcaaaaa 28500 aggctgtacg tcctgcacca gctggatagc tcggagctga attacaacct cccaagcgcc 28560 ttgcaattgg aaggggcttt gaacgaggcc aaagtggaaa aggcgctgac tactttggtg 28620 gcccggcacg atatgctgcg caccggtttt gaaatcgtga atggggagcc ggttcagcgt 28680 attcatccgc tcgtagcttt caaggtcgag aagcttcaag caagcgaaga tcaggttgcg 28740 gccattcttg aaggcttcat tcagcctttt gacttgaccc agccgccttt gctgcgcgcc 28800 ctactgatcg aactggagaa agagaaattc ctgctcgcgc tggatattca tcatattggt 28860 tccgacggcc tttccatgga cgtgctgctg cgcgaattcg tgcggcttta caatggggaa 28920 gaattgccgg agctgcggat tcaatacaag gattacgccg tttggcagca atccgaggaa 28980 cagcgccagc gcatcaaacg gcaggaggaa tactggcgcg gggtattcag ctccgagctt 29040 ccggttcttg agctgcctct cgacttctcc cgcccggccg tccagcagtt tgacggtcaa 29100 acgctcacgt ttacgctgga tgcggagaaa agcgaagctc tcaaacggct tgccggcgat 29160 tcgggggcga cgctgtacat gcttctgctg gctgcttact ccgtattgct tcataaatac 29220 gcgggacagg aagatatagt ggtcggcact ccgattgccg cccgttctca cgccgacctg 29280 cagccgatta tcggcatgtt cgtcaatacg ctggcccttc gcttgggtcc ggtggcggag 29340 cggacgttcc tggattactt gcaggaagtg aaggaaacga cgcttggagc ctacgagcac 29400 caggactatc cgttcgagga gctggtggaa gctcttcagg tgagccggga tttaagccgg 29460 aatcctctgt ttgacaccat gttttctttg caaaagcacg aaagcttgga tttaaccctg 29520 gaaggcttgc aatggtcgct gttcgacatc gaggaaaaga cggcaaagtt tgatcttagc 29580 tttgatatcg tggaagccga taacgagctg atttgcaaga tcgagtacgc tacctcgttg 29640 tttagacagg aaacgatggt acggctggcg ggtcattacg agcagctttt ggcgtcgatc 29700 ctggctcagc cgggtgcgcg gatttcggat ttggatatct tgacggacag cgaaaagcat 29760 gatttgctgg tcgggtttga cgtgtcgtct tcggctcttg cgaagcaacc cgccgcagag 29820 ggtacaggtt tggaagcgga tgagtcgtgg agagagagaa cgttccacga gctgttcgag 29880 gagcaggcgg agcgcactcc gggagcgctg gcggttgtct acgaagacag caagctgacg 29940 tatgcggagc tgaacgccaa agcgaatcgt ctggcgtatg cactgcgggc gcgcggggtg 30000 aagccggagc aggtggtcgg cattctggcc ggccgttcgg cggagctgtt gatcggggtg 30060 ctcgccgtat ggaaagcggg cggcgcttat gtgccgctcg acccggacta tccggcggag 30120 cggatcgagt atatgctcgc ggacagcggg gcgtcggtgc tgctcacgca gacccgcctg 30180 ctggagcagg cggaagcttg gcgcagcgac ggagctctag tgctgcaaac ggtgcttgcg 30240 cttgacgacg ccgcgacgta cagcctcgga gcggcggaag cggctgtggg cgaagcaggc 30300 gcagaggcgg aggctttggc gcaagcggct gctgccgaat cgtccgccac ggcagaagcc 30360 gagaagaacg tactagcggc ggatctcgca tcgaatccgg cgaatgtgaa caagccgcgc 30420 gatttggctt acgtcatcta cacctccggt acgacgggcc gtccgaaggg cgtggcggtg 30480 gaaaaccgca gcctggtgaa cacggcggcg ggctaccggc gggaataccg gttggatcag 30540 ttcccggtgc ggctgctgca gctcgcaagc ttctcgttcg acgtgttcgt gggagatatc 30600 gcgcggacgc tgtacaacgg cggcaccatg gtcatcgtgc cgaaggacga ccggattgat 30660 ccaacccgcc tctacggctg gattcgcgac tacgccgtga cggtgttcga atcgactccg 30720 gcgctgatcg tgccgttcat ggagcatgtg catgccgagg gtctggacct cagctcgatg 30780 cagctgctga tcacaagctc ggatgcgtgc agcgtagcgg attaccgcac cttgcaggag 30840 cgcttcggct cgcagttccg tattattaac agctacggcg taacggaagc ggcgattgac 30900 tccagcttct atgacgagcc gctggagaag ctgccgaaga cgggcagcgt gccgatcggg 30960 aaagcgtggc tgaacgccaa gttttacatc gtggatgcga atctgaagcc ggtgccgatc 31020 ggggtgttgg gcgagctggt tatcggcgga gcgggtgtgg cccgaggtta cttgaaccgc 31080 ccggatttga cggcggagaa attcgtagac agcccgttcg ccgctgggga gcggctgtac 31140 cggacgggcg acctggcgcg ctggatgccg gacggcaacg tggacttcat cggccggatc 31200 gacaaccagg tgaaaattcg cggctatcgg atcgagcttg gtgaaattga agcggccatg 31260 aaaaattttg ccggcgtgcg tcaagcgctt gtcatcgacc ggacggacga gcgggggcag 31320 aaatatttgt gcgggtatgt cgtagcggat tccagcttcg atctggaagg gcttgtggcc 31380 catctggacg ccgcgctgcc ttcccatatg gtgccttcgc gcatcatgcg cctggatcaa 31440 atgccgctta cgccgaacgg gaagatcgac cgtaaagcgc tgcctgtacc ggaaggaagc 31500 attcgtgccg aggctgcata cacggcgcct cgtactcctg ccgagcaagc acttgcgtcg 31560 gtctggcagt cagtgctggg cgtggatcag gtcggcacga tggacaattt ctttgcgctc 31620 ggcggcgatt cgatcaaggc cttgcaggta tcgtcccgtc ttttgcaaac ggggtacaag 31680 ctggtcatga aagatttgtt ccattacccg acgatttccg cccttagttt gcagctgcaa 31740 acggcggaga gaacggcaag ccaggccgaa gtgacgggag aggtcatctt gaccccgatt 31800 cagcgttggt tctttgaaca aaatccggcc gacgtgcatc acagcaacca ggcgttcatg 31860 cagttctcca agcaaggctt tgacgaagaa gctttacgcc aagcggtgcg tcagcttgtc 31920 gtgcatcacg atgctctccg tacggtttac cgccaaactg agaacggcta taccgcctgg 31980 aatcgcggcg ccggggagaa cgaagcgctg ttcgatctgg aagttgtcga tttcaaggga 32040 gtcggcgacg tgaaagaagc ggtagaggct aaggcgaatg acattcaagc gagcatcgat 32 100 ctggaaaacg gtccgctggt gaagctcggc ttgttccgct gcgacgacgg cgaccacctg 32160 ctcatcgcga tccatcactt ggtcgtagac ggcgtatcct ggcggattct gcttgaggat 32220 tttgctgcct gctatgagca ggcgctgcaa gggcagccga tccgtctgcc gctcaaaacg 32280 gattcattcc aaacgtgggc gaaacagctc gctgattatg cgaacggtcc ggcgatggaa 32340 agcgagagag agtattggca gcatatcgag caattgacct atgagccgct tccgaaagat 32400 tttgaacaag gcagatccaa gctgaaggac agcgggctcg tgaccgttcg ctggacggcg 32460 gaggaaaccg aacagctgct gaagcaggca cactgtgctt accatacgga aatgaacgat 32520 ttgctgcttg ccgcgcttgg cctcgcggta caagcttgga gcggccggga acgcgtgctg 32580 gtgaatctcg aaggccacgg ccgggaagat attttgccga atgtggacat tacgcgcacg 32640 gtaggctggt ttacaagcca attccctgtc gttctggagc cgggtcacgc ccaggcgctc 32700 ggtcatcagg taaaacaggt taaagaaagc ttgcgccgca ttccgaacaa aggaatcggc 32760 tacggcatcc tgcgttattt gtcggcgccg cgtgacggcg agcgcttcgc tttggagccg 32820 gagatcagct ttaactattt gggtcagttc gaccaggatt acgaaagcag cggctcgagg 32880 ccgtctccgt tcagcccggg ctccgattca agcccggatg cagtgatgga ttatgtcctc 32940 gatatcaacg gtatggtgtc ggaaggagtg caggaactca cgatccgtta tggggaaacc 33000 cagtataaac gggaaacggt agagcgcctg ggcaccctgc ttcattcgag cttgcgtgaa 33060 gtcatcagcc attgcgtatc gaaagagcgg ccggagctta cgcctagcga cgtactgctt 33120 caagatgtga cgttggagga actggagcgg ctggccgaac atacggcggc gctcggcgaa 33180 ctggagaatg tatacaccct gactccgctg caaaaaggga tgttgttcca cagcctgctg 33 240 gatgccgatt cggaagctta cttcgaacag gtgaccttcg atctgtacgg aagcctgaat 33300 gtcgaagcct tcacccaagg attggatacg ctggtgcagc ggaatgaggc gctgcgtacc 33360 aactttatta ccggctggag agacgagccg attcaagtgg tattccgcga gcggaagtgt 33420 gaagtgtact tcgaagatat tcgctcggta agcgatgaag acccggagaa gacgatagcc 33480 gatttcgtca gcgcggataa agcgaataag ttcgatttgg cccgaggctc tcttatgcgc 33540 gtgacagttt tgcgcacggg cgacgagtcc taccatgtga tctggagtca ccatcacatt 33600 ttgatggacg gctggtgcat gtccttcatg atcaaggaag tgttcgacac ctacttcgct 33660 ttccaggaga agcggacgct ggagcttcct ccggttacct cgtactcccg gtatatcgaa 33720 tggctggaag ctcaagatgc cgcgaaagct tcgcgttact ggtccgagta tttggcgggt 33780 tacgatcagc agaccaagct gccccaggag aaaacgcagc tgaagcaggg cgcttttgaa 33840 gcggctgaaa tcgatgtgga actcagcaag gaactgaccg ggcaaatcga gcgggtggcg 33900 cgccagcagc aggtgacgct caatacgttc atgcagaccg tatggggact ggttctgcag 33960 gtatacaaca acagcgagga tgtcgtattc ggctccgtcg tatccgggcg tccggcggaa 34020 attccgggca tcgaaagcat gatcggcctg tttattaaca cgatcccggt ccgtattcaa 34080 ggcaaagccg aggagacggt agccgatatc ttgagaaaaa cccaggatca agcgctggca 34 140 tcgggagctt acgaaacgtt cccgctgttc gaaattcagt cgctgagcga gcacaagcgc 34200 gacttgatca accatatcat ggtctttgaa aattatccga tggaagaaca gattgagcag 34260 gtcgtcggcg gagacaaaga agcgctgaaa atcgctaata tccagtcgcc ggagcaaacg 34320 aactacgacc tggacattac cgtcattccg gaagagcata ttttgctgcg gtttacgtac 34380 aatgcgctga cgttcagaga ggaagacatc aggctgatcc acggtcattt tgcccgggta 34440 ctggagaagg ttgcggctaa cccgagcatc cgcgtgaatc agttggagct tttgacggcg 34500 gcggaaaaag agcaaattct tggcgcgttt aacccggcgc agccggaagc ggctccggcg 34560 gccgcgttcc accggctgtt cgaggaacag gcggagcgca cgccggaagc ggcagctgtc 34620 gtgtacgaga atgaccggct gacgtatgag gagctgaacg agcgggcgaa tcgcttggcg 34680 gccacgctgc gcgcaagcgg catcggccgg gagacgatcg tcggcattct cgccgagcgt 34740 tcggtggact tgctggtggc cgtgctggcc gtctggaaag cgggcggggc gtatgtgccg 34800 ctcgacccgg attatccggc ggagcgggtg cggttcatgc tcgaagacag cggagcgaag 34860 gtgctgctga cgcaaacgcc gctgcgagag cgcgccgaag cctggctcgg cgaagaggag 34920 ctggcgctgg cggcggtgct gtacctcgac gacgaaactt cgtacagcga ggagcggacg 34980 aatgccgaca gcttccatga agcccgtcca gaggacctgg cgtacgtgat ctacacgtcg 35040 ggaacgacgg gcaagccgaa gggcgtgatg atcgagcacc gcagcctggt gaacacggcg 35 100 gcgggctacc ggcgggaata ccggttggat cagttcccgg tgcggctgct gcagctcgca 35 160 agcttctcgt tcgacgtgtt cgtgggagat atcgcgcgga cgctgtacaa cggaggtacg 35220 atggtgattg tgccgaagga cgaccggatc gatccgtctc gtctgcacca ctggatggag 35280 cgggagcggg tcaccatctt cgaatcgacg ccggcgctga tcgtgccgtt cctggagtac 35340 gtgcacgatc agcggctgga tatgagctgg atggagctgt tgatcacgag ctcggacagc 35400 tgcagcgtgg cggattaccg gaccttgcag gaacgcttcg gctcgttgtt ccggatcatc 35460 aacgcatacg gcgtgacgga agcggcgatc gactcaagct tctatgacga ggagctggcg 35520 aagctgccgc agacaggcaa tgtgccgatc ggcaaagcgt ggctgaatgc gaaattctac 35580 atcgtggacg cgcatctgaa cccggtgccg gtcggggtgc tgggcgagct ggttatcggc 35640 ggagtcgggg tagcgcgcgg gtacttgaac cgtccggagc tgacagaaga gaagttcgta 35700 gacagcccgt tcgccgcggg cgagcggctg taccgcacgg gagacttggc gcggtggatg 35760 gaggacggga acgtggactt catcggccgg atcgacaacc aggcgaaaat ccgggggtac 35820 cggattgaga cgggcgaagt cgaagcgaag ctgctgagtg taggtggcgt gaaggaagcg 35880 gtcgtcgtcg tcagggaaga tcaagaaggt cagaaagctt tgtgcgctta ttatacagcg 35940 gaagaaggct tgacggcggc ggacctgaag cgggcgattg ccaacgagct gccggggtac 36000 atgatcccgt cgtacttcgt ggagctggag cacctgcctc tgacgccgaa cgggaaaatc 36060 gaccggaagg cgctgccggc gccggaaggg ggagcaagcg caggccgcga atacgtggcg 36 120 ccgcgcaccg aacttgaggc gaagctggcc gccatttggc aggatgtgct tgttcgggag 36180 aaggcggtag gcgtaacgga caacttcttc gacctcggcg gacactccct gcgggcgacg 36240 acgcttgtca gcaaaatgca taaggagcta ggcgttgaat ttccgcttcg cgacgtattc 36300 cgctactcga cggttgagga aatggccgcg gctatggagc ggctggagat cggctcgttc 36360 acggctattc cggctgcgga acctagcgag tattatccgc tctcttccgc tcagaaacgt 36420 ctctatatct tgaaccagct ggaaggagcc gagctgagct acaacatacc gggagctatg 36480 ctgctcgaag gggagctcga ccggcagcgg tttgaagaag cgttccgcgg gctcgtagct 36540 cgtcatgaaa cgctgcgtac cggctttgag atggtaaaag gcgaagcggt tcagcggatt 36600 tacgaagaag ccgccttcca ggtggaatat gtgcagatca gcgcggagca ggcggaagaa 36660 acggtgcgcc aattcgttcg tccgtttgat ctagcgaagc cgccacttct gcgggtgggc 36720 cttgccgaac tggcgccgga ccggcacatt ctaatgttcg atacgcatca tatcgtatct 36780 gacggcgttt cgattgacgt actgatcgaa gagttcgtcc gcttgtacag cggggagcag 36840 ttggagccgc tccgcattca gtacaaagat tatgcggtat ggcagcaatc ggacgagcag 36900 aaagctcagc ttgccaagca ggaagcctac tggctcgaca tgttccgcgg agagctgccg 36960 gttttggaat tgccgatgga ctatccgcgc ccggctatgc agagctacga gggccgcacg 37020 ctgcaattgt ttatgcatag cgagaaaagc gagggtctga aacggcttgc agccgagaac 37080 ggcgcaacgc tttacatggt tctgcttgct ggttatacaa tattattgca taaatataca 37140 gggcaagaag acgtggtggt cggtacgccg attgcgggaa gaaatcacag cgacgttcag 37200 ccgctgatcg ggatgttcgt caataccctg gccatccgca gttatccggc tgccggtaag 37260 acgttccttg actacttgaa ggaaatcaag gagacgacgc tgggcgcttt tgaacatcag 37320 aattatccgt ttgaggaact ggtggataag gtgaacgtgg ctcgcgattt aagccgcaat 37380 ccgctgttcg atacgatgtt tgctttgcag aatacagaga atttggaaat ccagcttccc 37440 ggactccatt tgtcgacgta tgccagcgaa gaaattgttt ctaaattcga tctcagcttg 37500 gacgtcacgg agatcgagga aggcttggaa tatctgtttg aatacgccac tgctctttat 37560 aaaaccgaaa cggtggagaa attggccgct cactacttgc agctgcttga atccattctc 37620 cgcaacccgt cggcgactat tgccgagctg ggcattttga caccggcgga aaaagagcaa 37680 attctcggcg cgtttaaccc ggcgcagccg ggagcggctc ctgcggctgc gttccaccgg 37740 ctgtttgagg aacaggcgga gcgcacgccg gaagaggcgg ccgtcgtgta cgagaacgac 37800 cggctgacgt atgcggagct gaacgagcgg gcgaaccgct tggcggctac gctgcgcgca 37860 agcggcatcg gccgggagac gatcgtcggc attctcgccg agcgttcggt ggacttgctg 37920 gtggccgtgc tggccgtctg gaaagcgggc ggggcgtatg taccgctcga cccggattat 37980 ccggcagacc gcgtgcggtt catgcttgaa gacagtggag cgaaggtgct gctgacgcaa 38040 acgccgctgc gagagcgtgc cgaagcctgg ctcgacgaag aggagctggc gctggcggcg 38 100 gtgctgtacc tggacgacga agcgtcgtac agcgaggagc gggcgaatgc gtcggttgtc 38 160 tccggcattg tctccggcat tgtctccggc aagctgacgg atgctgtgga cgacggcgat 38220 gagagccatc agaatgttgg caccgacagc ttccatgaag cccgtccgga ggatctggcg 38280 tacgtgatct atacgtcggg aacgacgggc aagccgaagg gcgtgatgat cgagcaccgc 38340 agcctggtga acacggcggc gggctaccgt cgggaatacc ggttggatca gttcccagtg 38400 cgcctgctgc agctcgcaag cttctcgttt gacgtgttcg tgggagatat cgcccggacg 38460 ctgtacaacg gaggtacgat ggtgatcgtg ccgaaggacg accggatcga tccgtctcgt 38520 ctgcaccact ggatggagcg ggagcgggtc accatcttcg aatcgacgcc ggcgctgatc 38580 gtgccgttcc tggagtacgt gcacgagcag caactggata tgagctggat ggagctgttg 38640 atcacgagct cggacagctg cagcgtggcg gattaccgga ccttgcagga acgcttcggc 38700 tcgttgttcc ggatcatcaa cgcatacggt gtgacggaag ctgcgatcga ctcaagcttc 38760 tatgacgagg agctggcgaa gctgccgcag acaggcaatg tgccgatcgg caaagcgtgg 38820 ctgaatgcga aattctacat cgtggacgcg catctgaacc cggtgccggt cggagtgctg 38880 ggcgagctgg ccatcggcgg agtcggagtg gcgcgcgggt acttgaaccg tccggagctg 38940 acggaagaga agttcgtaga cagcccgttc gccgcgggcg agcggctgta ccgcacggga 39000 gacttggcgc ggtggatgga ggacgggaac gtggacttca tcggccggat cgacaaccag 39060 gcgaaaatcc gggggtaccg gattgagacg ggcgaagtcg aagcgaagct gctgagtgta 39120 ggtggcgtga aggaagcggt cgtcgtcgtc agggaagatc aagaaggtca gaaagctttg 39180 tgcgcttatt atacagcgga agaaggcctg acggcggcgg acctgaaacg ggcgattgcc 39240 agcgagctgc cggggtacat gatcccgtcg tacttcgtcg agctggagcg cctgcctctg 39300 acgccgaacg ggaaaatcga ccggaaggcg ctgccggcgc cggaaggggg agcaagcgca 39360 ggccgcgaat acgtggcgcc gcgcaccgaa ctggaggcga aactggtcgc catctggcag 39420 gacgtgctcg ggccggtcac gattggcgtg acggacaact tcttcgacct cggcgggcac 39480 tccctgcggg cgacgacgct ggtcagcaag gtgcacaagg agctgagcgt ggacctgccg 39540 ctgcgcgatg tgttccggca ctcgaccatc gaagcgatgg ccgaagcgat cagccaattg 39600 gagcggcagg aacacctctc cattccggtt ctggataaga gggattacta tccgctttcc 39660 tccgtgcaaa aacggctgta catccagcag cagatggaag gcgccgagct tagctacaac 39720 atgtccggca tgacggttct cgtcgggcgc ttggaacgga atcaattcga ggcggcgctc 39780 aaaggattga tagctcgtca cgaaattttg cgaaccggct tcgaaatggt cgacggcgaa 39840 ccggtacaac ggatttatcc ggacttgaag tttgccgtcg agtatacgaa agcgacggaa 39900 agtgaaacga agagcatcgt agacggcttt gtgcgcgtct ttgatttgga gcggccgccg 39960 ctgctgcgtg tgggcttagt cgaatgggaa gcggaacggc atctgctcat gctggacatt 40020 catcatatcg tcacggatgg catgtcgatg ggcatcttcg tcgaagagct gctgcgcctg 40080 tataacggcg agactatgga accgcttcgg attcaataca aggaattcgc cgcttggcag 40140 cagtccgaac ctgtaaaaga gcggctgaaa cgtcaggaag cctactggct ggacgtgctg 40200 gaaggcgaac tgccgacgct tgaactgcct acggactttg tcagacctgc cgctcgcagc 40260 tttgagggag atgtgctgcc tttcagcatc gacaagcaga tgaccgacag cttgcagcgc 40320 atcgccgatg agaacggtgc caccctttat atggtgttat tggcggccta ttcaatcctg 40380 ctcagcaagt actcgggaca agaagacttc attgtaggca cgccggtttc gggccgcaca 40440 catgccgacc tggagccgct catcggaatg tttgtcaaca ctttggcgat tcgccattat 40500 ccgtccgggg agaagacgtt cctcgcttac ttgaacgaag tcaaagaaac gatgctgggg 40560 gcctacgacc accaggatta tccgttcgag gagcttgtga aaaagctgca ggcgccgcga 40620 gatcaaggcc gcaatcctgt attcgatgtc atgtttgctc tggaaaccaa ggaagataac 40680 gttcaaagct tcggggatat caagatcgaa tcttatccgg aaactcatac ggtttcccaa 40740 tttgatctta ccttggtcat ttcgttgctg gatgagggaa tgaacgggca gtttgaatat 40800 gccaccaagt tgtttacacg caatctgatc gacaatttcg ctcaggacct gctcgtgatc 40860 atcacccaaa tttgcgaaca gccttcggtg ctgttgaagg atatttccct gaacgggcaa 40920 tccgaacagg agcaagatgt gctagagacc attgatatta ttttc 40965

Claims

An isolated polymyxin consisting of a PmxA polypeptide subunit having an amino acid sequence as set out in SEQ ID NO: 4, a PmxB polypeptide subunit having an amino acid sequence as set out in SEQ ID NO: 5 and a PmxE polypeptide subunit having an amino acid sequence as set out in SEQ ID NO: 6 E biosynthetic enzyme.

A polynucleotide encoding the polymyxin E biosynthetic enzyme of claim 1.

Article characterized in that it comprises a pmxE gene having a nucleic acid sequence that is 2 wherein, the substrate to pmxB gene and SEQ ID NO: 14 has a nucleic acid sequence represented by pmxA gene, SEQ ID NO: 13 has a nucleic acid sequence represented by SEQ ID NO: 12 to Polynucleotide.

4. The polynucleotide of claim 3, wherein the pmxA, pmxB and pmxE genes are in the form of gene clusters.

5. The method of claim 4, pmxD having a nucleic acid sequence described in SEQ ID NO: 16 pmxC gene and having the nucleic acid sequence represented by SEQ ID NO: 15 Polynucleotide further comprises a gene.

An expression vector comprising the polynucleotide of any one of claims 2 to 5.

A transformed host cell prepared by transducing the expression vector of claim 6 non-chromosomal.

The transformed host cell prepared by inserting the polynucleotide of any one of claims 2 to 5 into the host cell genome.

A method for producing polymyxin E, comprising culturing the transformed host cell of claim 7 in a nutrient medium.

10. The method of claim 9, further comprising the step of separating and purifying the polymyxin E.

A method for producing polymyxin E comprising culturing the transgenic host cell of claim 8 in a nutrient medium.

12. The method according to claim 11, further comprising the step of separating and purifying polymyxin E.