KR20110109423A - Composition of chicken forage - Google Patents

Abstract

The present invention is a feed composition of a chicken prepared by combining an antioxidant propolis and an excellent detoxification citric acid to a top bio, a chicken feed manufactured with fish by-product powder as a main raw material, 90 to 99% by weight of top bio and propolis powder It is prepared by mixing 0.9 to 9% by weight and 0.1 to 1% by weight citric acid powder.

Description

Composition of Chicken Forage

The present invention relates to a chicken feed composition prepared using Top Bio, propolis and citric acid as the main ingredients, and more specifically, propolis which is an antioxidant to Top Bio, a chicken feed prepared using fermented rice powder as the main raw material. It relates to a chicken feed composition prepared by combining citric acid with a detoxifying ability.

Avian Influenza (AI) is a contagious respiratory disease that affects birds. Once it occurs, it spreads across the oceans and continents through migratory birds, and is called the catastrophe of humanity. One example is the Spanish flu (H1N1), which occurred during the First World War in 1918, killing 40 to 50 million people.

All avian influenza known to date belong to one species, influenza A. There are several subspecies of influenza A virus, such as H1N1, H5N1, and H7N1. The problem is that avian influenza can be a new strain of airborne transmission from person to person like the common flu.

In Japan, among them, H5N1 and H7N1 are classified as H5 subtype and H7 subtype as highly pathogenic avian influenza, and specially managed.

In the meantime, measures to prevent the spread of avian influenza include the early detection of viral invasion in poultry farms, screening, etc., early detection of virus invasion, use of inactivated vaccines, and disinfection to purify avian influenza virus. It costs a lot, and the efficiency is bad.

Therefore, there is an urgent need to develop a method that can effectively block the occurrence of avian influenza as efficiently as possible.

The problem can be solved by feeding the poultry chicken feed containing antiviral, immune vitality supplements to increase the immunity to the chicken.

Feeding the feed of the present invention containing essential nutrients, antiviral and immune vitality supplements necessary for chickens to chickens for a long time can increase chicken's stamina and immunity, thereby enhancing immunity against avian influenza.

Figure 1 shows the results of measuring white blood cell counts for the control and combination groups
Figure 2 shows the results of measuring lymphocyte counts for the control and combination groups.
Figure 3 is a chart showing the results of measuring mononuclear leukocyte counts for the control and combination groups.
Figure 4 shows the results of measuring SOD enzymes for the control and combination groups.

The present invention relates to a feed composition of a chicken prepared by combining an antioxidant propolis and an excellent detoxification citric acid to Top Bio, a chicken feed prepared from fish by-product powder as a main raw material.

Top Bio is a chicken feed developed by the present inventors is prepared by mixing the dried soybean skin powder, dried fish by-product powder, charcoal powder, mineral and seaweed powder.

Soy skin is rich in fiber and contains crude protein, crude fat and ash, making it suitable for animal feed.

Top Bio contains 32 to 35% by weight of bean husk powder.

 Fish is rich in unsaturated fatty acids and quality proteins, especially back blue fish is rich in omega-3s, so fish by-products are suitable for animal feed. Top Bio contains 50-50% by weight of dried fish by-product powder.

 Charcoal is rich in minerals in addition to air purification, far-infrared radiation, disease treatment and detoxification. Minerals contained in charcoal are calcium (Ca), sodium (Na), iron (Fe), magnesium (Mg), potassium (K), and phosphorus (P). Top bio is blended with 3 to 5% by weight of charcoal powder.

 Minerals are essential ingredients in the production and activation of enzymes that play a crucial role in energy production. Lack of minerals in the body leads to lethargy. Therefore, it is an essential ingredient in animal feed. Top Bio contains 8 to 10% by weight of minerals.

 Numerous minerals, polysaccharides, proteins, and vitamins in algae play an important role in maintaining health in human cells and blood. Top bio is blended with 9 to 10% by weight seaweed powder.

In the present invention, 90 ~ 99% by weight of Top Bio, a chicken feed prepared by mixing the dried soybean hull powder, dried fish by-product powder, charcoal powder, minerals and seaweed powder as described above.

Propolis is a natural antibiotic extracted from honeycomb and consists of resins, waxes, flavonoids, essential oils, various vitamins, minerals and amino acids.

Propolis contains the most organics and minerals (inorganic salts), and contains 104 kinds of ingredients. Among many components, mineral vitamin amino acid fatty acid flavonoids play an important role in cell metabolism, and terpenes have anticancer activity. In particular, there are more than 100 kinds of flavonoids, which are very helpful for promoting health.

The main efficacy is anti-inflammatory acid screen. The anti-inflammatory effect is due to half the enzymes that make prostaglandins that cause inflammation in the body. In addition, the flavonoids, the main ingredient, eliminates free radicals, which have antioxidant effects. Quercetin, which has anti-cancer effects, blocks cancer genes before they are replicated.

In the present invention, 0.9 to 9% by weight of propolis powder was blended.

Citric acid (CHO) is contained in plums, citrus fruits, lemons and strawberries with strong sourness, and is used as acidic acid in soft drinks. Citric acid, on the other hand, strengthens the constitution by activating macrophages, anti-aging of the skin, anti-aging of the bones, and lipocytolysis, which is the core of the immune system, and promotes sterilization, detoxification, sedation, constitution improvement, and liver function. Prevents acidification of blood, inhibits calcium stones, prevents athlete's foot, promotes saliva secretion, and promotes secretion of gastric juice.

 In this invention, 0.1-1 weight% of citric acid powders were mix | blended.

Therefore, the chicken feed of the present invention is prepared by blending 90 to 99% by weight of top bio, 0.9 to 9% by weight of propolis powder and 0.1 to 1% by weight of citric acid powder.

By feeding the feed formulated as above to feed the chickens, including avian influenza to strengthen the resistance to various diseases.

Hereinafter, the effects of antioxidant activity, immune vitality, and blood cell counts in the peripheral blood of the compound of the present invention through the experimental examples were measured, and the activity and antioxidant activity of total T lymphocytes were measured.

Experimental Example 1

1-1. Experimental material

 As experimental animals, ICR mice (rats) of 5 weeks old were used for experiments after 11 days of preliminary work. That is, 98.9 weight of the top biopowder in the control group to which no ICR mouse was administered, the propolis group administered only a propolis solution dissolved in a solvent, the citric acid group administered only a citric acid solution dissolved in a solvent, and the solvent % And 1% by weight of propolis powder and 0.1% by weight of citric acid powder were classified into a top biopropolis citric acid compound group (hereinafter, referred to as a 'compound group') to which a solution was dissolved. In addition, the irradiation group irradiated with 2GY (high dose radiation) was classified into a control group and a top biopropolis citric acid compound group. The concentration of the solution was set at 500 mg / kg.

1-2 administration method

  A solution of the propolis solution, citric acid solution, and the top biopropolis citric acid mixture was subjected to forced oral administration by gastric soda method daily to each group of 1-1. The control group received the same amount of distilled water equally.

Dosage depends on body weight change, so the body weight was measured every 3 days and the dose was adjusted according to the body weight.

1-3 Irradiation method

 In order to investigate the functional exertion in the condition of lowering immunity, the following experiment was conducted. That is, two weeks after the solution of the top biopropolis citric acid mixture and distilled water were administered to the blending group and the control group, a 2GY dose rate (0.35GY / mln) was applied to the blending group and the control group using the Philips X-ray irradiation apparatus (225kv). Systemic irradiation

1-4 Blood count

 The tail vein of the mouse was excised to collect 10 μl Caliberated Pipets (manufactured by Doramond), and then the white blood cell count and lymphocyte count were measured using an automatic hemocytometer (Celitac-α MEK-6318, Japanese photoelectric company). In addition, for the non-irradiated group not irradiated with X-rays to measure changes over time, 2,4,6,8 weeks after the start of the administration, and irradiated groups irradiated with X-rays on the day before irradiation, irradiation 2, After 12,24 hours, blood was measured after 2,7,14,30 days.

1-5 Determination of SOD Enzyme Activity

 SOD test kit (Wako Pure Chemical Industries, Ltd.) was used for the measurement of SOD enzyme activity. Blood was collected from the fundus of the ICR mouse by using a red blood cell volume capillary tube, and serum was separated by a capillary centrifuge. 80 µl of the luminescent reagent and 80 µl of the enzyme solution were added to 8 µl of the obtained serum, and the mixture was warmed at 37 ° C for 20 minutes. After that, 1.6 ml of the reaction stopper was added, and the absorbance was measured by Multiskan JX (Thermo) to request the SOD activity value.

1-6 Flow Cytometer Measurements

 Blood was collected from the heart of the ICR mouse and treated with heparin to prevent blood coagulation. Then, the erythrocyte removal reagent was mixed and washed, and the cell number was counted and analyzed by fluorescence staining. The measurement time was 30 days after irradiation, and the treatment used whole blood method.

1-7 Hemolysis Process

 1 ml of collected blood was added to a 15 ml parcon centrifuge tube containing 14 ml of a red blood cell removal reagent (ammonium chloride hemolytic agent) stored at room temperature, mixed well, and the mixed solution was left for 3 minutes. Then, the mixture was stirred at 300 G (1150 rpm) for 5 minutes and centrifuged at room temperature (HEMAT CRIT KH-120, 久保 田 商社) to remove the supernatant. Next, the cooled buffer (PSB (-) containing 0.1% sodium azide) was added, mixed at room temperature, stirred at 300G (1150 rpm) for 5 minutes with a cooling centrifuge, and centrifuged at 2 to 8 ° C. The supernatant was then removed and re-clouded with 1 ml of color buffer (PBS (-) containing 0.1% sodium azide and 2% FBS). The number of cells per test object was 1 × 10 Cell or less in order to count the number of live cells so that the test object contains 90% or more live cells and prevent the appearance of unstained cells due to the amount of antibody. .

 1-8 Dyeing Process

 50 µl of the adjusted cells were taken from the parcon tube, alternately added, and reacted with cold dark for 30 minutes. Next, 2 ml of the washing buffer was added and mixed at room temperature, followed by stirring at 200 G (950 rpm) for 5 minutes, followed by centrifugation at room temperature to remove the supernatant.

0.5 ml of PBS (phosphate-buffered saline) solution was added thereto, mixed well, and stored at 2 to 8 ° C.

1-9 Avian Influenza Virus Infection

 Avian influenza virus (H5N1) was infected after ingesting the corresponding solution, such as distillate or propolis, for two months or more according to the administration method of 1-2 in each group classified in 1-1 in an isolated poultry farm. Survival was checked after 1 week (?).

2. Experimental results

2-1. Removal rate of avian influenza virus (H5N1)

Avian Influenza Virus
(H5N1) Removal rate Control group Propolis County Citric acid Top Biopropolis Citric Acid Blend 0% (010⁴ / 21010⁴) 86.66% (18210⁴ / 21010⁴) 95.71% (20110⁴ / 21010⁴) 88.57% (18610⁴ / 21010⁴)

As shown in Table 1 above, avian influenza virus was not removed at all in the control group administered with distilled water, but at 86.66% in the propolis group. 95.71% of citric acid was removed. On the other hand, the compounded group also showed a high removal rate of 88.57%.

2-2. Immunity enhancement

2-2-1 white blood cell count

 The results of measuring white blood cell counts for the control group and the combination group are shown in FIG. 1. As shown in Figure 1 it can be seen that the immunity was significantly enhanced in the combination group after 4 weeks.

2-2-2 lymphocyte number

 The result of measuring lymphocyte counts for the control group and the blended group is shown in FIG. 2. As shown in FIG. 2, the number of lymphocytes was significantly reduced in the blended group after 4 weeks.

2-2-3 Monocyte count

The results of measuring mononuclear leukocyte counts for the control group and the combination group are shown in FIG. 3. As shown in FIG. 3, when the avian influenza virus penetrated the body, the number of mononuclear leukocytes decreased until 2 weeks in the control group, and gradually increased after 6 weeks. It can be seen that the avian influenza virus has penetrated the body continues to increase. However, the number of mononuclear leukocytes increased after 4 weeks in the combination group, but gradually decreased after 6 weeks. This shows that the avian influenza virus that has penetrated the body is disappearing.

2-2-4 Activation of SOD (Antioxidant) Enzyme

As a result of measuring the SOD enzyme for the control group and the combination group is shown in FIG. As shown in Figure 4 it can be seen that the activation of the SOD enzyme is high in the top biopropolis citric acid mixed group.

2-2-5 Measurement results of CD4, CD8 and CD16

 end. If you did not investigate 2GY

If 2GY is not investigated, the measurement results of CD4, CD8 and CD16 are shown in Table 2.

County CD4 CD8 CD16 Control group 14.5 + -3.2 14 + -2.7 8.5 + -2.3 Formulation 22.3 + -4.5 17.2 + -3.8 41.8 + -6.5

 I. When irradiated with 2GY (high dose radiation)

In the case of 2GY, the measurement results of CD4, CD8 and CD16 are shown in Table 3.

County CD4 CD8 CD16 Control group 10.5 + -2.5 8.5 + -2.2 10.3 + -2.5 Formulation 15.2 + -2.6 12.6 + -4.1 22.5 + -7.3

In the case of irradiating 2GY and not irradiating 2GY, the combination group showed an increase in the measured values of CD4, CD8 and CD16, indicating that the immunity was increased.

2-2-6 Anti-Avian Influenza Effects on Combined Feeds

In the isolated poultry farm, each group classified in 1-1 was fed a normal feed for the control group, and the propolis group, the citric acid group, and the blended group of the present invention were fed with the avian influenza virus (H5N1). Infected. The results of the survival rate after one week (?) Are shown in Table 4.

Avian Influenza Virus
(H5N1) Infection Rate (%) Control group Propolis County Citric acid Mixed military 100% (10 匹 / 10 匹) 60% (6 匹 / 10 匹) 20% (2 匹 / 10 匹) 10% (1 匹 / 10 匹)

That is, when the feed compound of the present invention is fed a strong resistance to avian influenza it can be seen that the infection rate is significantly lower than the control group.

3. On the other hand, to investigate the antimicrobial activity, Werech and Gram-positive bacteria and Escherichia coli (hereinafter referred to as "Weursch") were also investigated by the agar plate inversion method. In addition, the effect of bacterial coexistence on the antimicrobial activity of the bacteria was examined by using a sensitive disk and agar plate layering method.

As in the case of 1-1, the subjects of the study were the control group without any administration, the propolis group with only the propolis dissolved in the solvent, the citric acid group with only the citric acid dissolved in the solvent, and the top bio and Four classes of formulation groups in which a solution in which propolis and citric acid are combined and dissolved is administered.

3-1 Determination of growth inhibition against Welsh bacteria

3-1-1 Measurement of Welsh germ growth inhibition

 Weerch bacteria are plated with platinum teeth on a Mueller-Hinton agar medium in which the agar concentration is adjusted to 3% in order to suppress the Weirsch fungal activity. Time incubation.

Afterwards, the agar was inverted aseptically using a spatel, incubated for 24 to 48 hours at 25 ° C, using a Weirsch bacterium, and the growth inhibition diameter of the Welsh bacterium as an indicator was measured. That is, in the vicinity of the intersection with the Welsh bacterium, a large number of indicator bacteria have been developed, and a portion where the growth of the indicator bacteria is not even in one group (well-prone state), that is, the growth inhibition diameter is less than 10 mm. The strain of-), the growth inhibition diameter of 10mm or more was called a strain of growth inhibition (+). In addition, it was compared by incubating for 24 hours and 48 hours.

Inhibition effect of antibacterial activity to each group by 3-1-2 Wersch bacteria (layered method)

 On the surface of the Mueller-Hinton agar medium with a diameter of 150 mm, the Weirsch bacteria were cultured at 108 ° C for 24 hours in a homogenous smear (108 cfuml) according to NCCLS. Subsequently, a Mueller-Hinton agar medium containing 5% sheep fiber fiber with 85% diameter was placed on the surface, and the germs were spread on the surface of NCCLS. Was measured.

As a control, a Mueller-Hinton agar medium was placed on 5% sheep fiber fiber on Mueller-Hinton agar medium without Welsh germ, and a susceptible disc was smeared according to NCCLS, and a susceptible disc was measured.

The statistically significant difference test for stopping circle diameter was performed using Wilcoxon test.

4. Experimental Results

4-1 Confirmation of growth inhibition of Welsh bacteria by agar plate reversal method

In the simultaneous detection of Welsh bacteria by agar plate inversion method, it was confirmed that the growth of Welsh bacteria was suppressed as shown in Table 5 except for the control group.

Type of bacteria Loss rate Control group Propolis County Citric acid Combination Welsh 0% (0/203) 86.9% (176/203) 97.04% (197/203) 91.13% (185/203) Gram-positive bacteria 0% (0/67) 82.08% (55/67) 94.02% (63/67) 91.04% (61/67) Escherichia coli 0% (0/65) 95.38% (62/65) 98.46% (64/65) 89.23% (58/65)

4-2 Confirmation of antimicrobial activity of Welsh bacteria by agar plate inversion

 In simultaneous detection of Welsh bacteria by agar plate inversion method, it was confirmed that the growth of Welsh bacteria was suppressed as shown in Table 6 except the control group.

Type of bacteria Loss rate Control group Propolis County Citric acid Combination Welsh 0% (0/182) 91.75% (167/182) 94.50% (172/182) 93.40% (170/182) Gram-positive bacteria 0% (0/65) 78.46% (51/65) 92.30% (60/65) 87.69% (57/65) Escherichia coli 0% (0/73) 73.79% (54/73) 97.26% (71/73) 91.78% (61/73)

Therefore, the present invention provides a chicken feed composition, characterized in that the top bio-90 ~ 99% by weight, the propolis powder 0.9 ~ 9% by weight and citric acid powder 0.1 ~ 1% by weight of the formulation.

Claims (2)

Chicken feed composition, characterized in that the top bio-90 ~ 99% by weight, propolis powder 0.9 ~ 9% by weight and citric acid powder 0.1 ~ 1% by weight
The method of claim 1, wherein the top bio is
32 to 35% by weight of soybean skin powder, 50 to 50% by weight of fish by-product powder, 3 to 5% by weight of charcoal powder, 8 to 10% by weight of minerals and 9 to 10% by weight of seaweed powder Chicken feed composition

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