KR20110088733A - A composition for diagnosing and treating cancer comprising nadph oxidase complex protein - Google Patents

Abstract

PURPOSE: A composition containing NADPH oxidase composite protein is provided to induce apoptosis of cancer cells and to treat and diagnose cancer. CONSTITUTION: A composition for diagnosing cancer contains polypeptides selected from the group consisting of NADPH oxidase composite(Nox) 1, NADPH oxidase complex(Nox)4, and NADPH oxidase composite(Nox) 5, and functional mutants as an active ingredient. The Nox 1, Nox 4, and Nox 5 have an amino acid sequence of sequence numbers 19, 20, and 21.

Description

A composition for treating and diagnosing cancer using the NADPH oxidase complex protein as an active ingredient {A COMPOSITION FOR DIAGNOSING AND TREATING CANCER COMPRISING NADPH OXIDASE COMPLEX PROTEIN}

The present invention relates to compositions and methods for treating and diagnosing cancer, and more particularly to treating cancer comprising NADPH oxidase complex family proteins and / or diphenylene iodonium, and / or derivatives thereof as an active ingredient. And a composition for diagnosing.

Malignant tumors (cancer) are the second leading cause of death after heart disease in the United States (Boring et al., CA Cancer J. Clin. 43: 7 (1993)). Cancer causes the blood or lymphatic system to increase by increasing the number of abnormal or neoplastic cells (proliferating to form tumor masses) derived from normal tissue, invading these neoplastic tumor cells into adjacent tissues, and a process called ex It is characterized by the development of malignant cells that eventually spread to local lymph nodes and distal. Cancerous cells grow even under conditions that normal cells cannot grow. Cancer itself manifests itself in a wide variety of forms, characterized by different degrees of invasiveness and aggressiveness.

Breast cancer is the most common malignant tumor of women in most industrialized countries, as it is estimated to develop about 10% of the population during their survival. Although its mortality rate has not increased with its incidence, thanks to early diagnosis and improved treatment, it is still a major cause of death in middle-aged women. Despite the early diagnosis of breast cancer, about 1 to 5% of women with newly diagnosed breast cancer have distant metastases at diagnosis. In addition, about 50% of patients with locally diagnosed local diseases eventually relapse into metastases. 85% of these relapses occur within the first five years after the initial onset of the disease.

Most patients with metastatic breast cancer initially have only one or two organ systems involved. As the disease progresses over time, a number of sites are generally involved. Indeed, metastases can be found in almost every organ of the body at necropsy.

The most common sites of metastatic involvement observed are the soft tissues of the skin and chest wall and the local recurrences of the armpits, and the area above the clavicle. The most common site for distant metastasis is bone (30-40% of distant metastasis), followed by lungs and liver. Metastatic breast cancer is generally considered an incurable disease. However, currently available therapeutic options often prolong disease free status and overall survival and improve quality of life. The average survival time after the onset of distant metastasis is about 3 years.

Although significant progress has been made in understanding the genetic changes that lead to cancer (eg breast cancer), the lack of reliable tumor assays for de novo human cancer cells is incapable of understanding the effects of these mutations at the cellular level. Interfered. In addition, the lack of identified cancer markers for solid tumor stem cells has hampered the development of diagnostic and therapeutic agents for cancer patients (eg, breast cancer patients).

There are also a huge variety of cancers described in detail in the medical literature. Examples include cancers of the lung, colon, rectum, prostate, breast, brain and intestine. The number of cancers continues to rise as the general population grows older, new cancers develop and susceptibility populations increase (eg, those infected with AIDS or overexposed to sunlight). Thus, there is a tremendous need for new methods and compositions that can be used to treat patients with cancer.

Well-known cancer treatments may include surgery, chemotherapy, hormone therapy and / or radiation therapy to eradicate tumor cells in a patient (eg, Stockdale, 1998, Medicine, vol. 3, Rubenstein and Federman, eds). , See Chapter 12, Section IV). Recently, cancer treatment can also include biological treatment or immunotherapy. All these approaches have significant drawbacks for patients. For example, surgery may be contraindicated due to the patient's health or may be difficult for the patient to accept. In addition, surgery may not completely remove tumor tissue. Radiation therapy is effective only when tumor tissue exhibits higher sensitivity to radiation than normal tissue. Radiation therapy can also often lead to serious side effects. Hormonal therapy is rarely given as a single factor. Although hormone therapy can be effective, it is often used to prevent or delay the recurrence of cancer after other treatments remove most of the cancer cells. Biological treatments and immunotherapy are limited in number and can cause side effects such as rashes or swelling, flu-like symptoms including fever, chills and fatigue, digestive tract problems or allergic reactions.

With regard to chemotherapy, there are a variety of chemotherapy agents that can be used to treat cancer. To prevent DNA replication and simultaneous cell division, most cancer therapeutics act by inhibiting DNA synthesis, either directly or indirectly by inhibiting biosynthesis of deoxyribonucleotide triphosphate precursors (Gilman et al., Goodman and Gilman's: The Pharmacological Basis). of Therapeutics, Tenth Ed. (McGraw Hill, New York).

Although various chemotherapeutic agents can be used, chemotherapy has many drawbacks. Stockdale, Medicine, vol. 3, Rubenstein and Federman, eds., Ch. 12, sect. 10,1998. Almost all chemotherapy agents are toxic, and chemotherapy causes significant and often dangerous side effects, including severe nausea, myelosuppression, and immunosuppression. In addition, even with the combined administration of chemotherapeutic agents, many tumor cells are resistant or develop resistance to chemotherapeutic agents. In fact, cells that are resistant to certain chemotherapeutic agents used in therapeutic protocols often prove to be resistant to other drugs, even if such agents act by mechanisms different from the drugs used in a particular treatment. This phenomenon is called pleiotropic drug or multidrug resistance. Because of drug resistance, many cancers are subject to standard chemotherapy treatment protocols.

It turns out to be intractable.

Meanwhile, the number of cancer (malignant neoplasm) deaths in Korea was 62,887, which was 25.5% (29.6% of male deaths and 20.5% of female deaths) among the total 246,515 deaths in Korea in 2002. Occupies. This means that about 131 people die of cancer per 100,000 people in Korea. From the point of view of cancer treatment, one of the biggest reasons for not reducing the mortality rate of cancer patients is due to the failure to suppress the induction or invasion and metastasis of cancer cells during malignancy. In the United States, recent statistical data show that metastatic cancer has not improved survival over the past five to five years. Therefore, in the US, the cancer treatment strategy is set to increase cancer treatment rate by suppressing cancer metastasis. Therefore, the understanding of the molecular mechanism of resistance to anticancer drugs and the invasion of cancer cells, which are characteristic of the malignant tumor process, will provide a foundation for the prevention and treatment of cancer metastasis. .

Epithelial-mesenchymal transition (EMT), on the other hand, refers to a series of processes in which epithelial cells are transformed into mesenchymal-derived cells such as fibroblasts (Kalluri, R., and Weinberg, RA (2009). J. Clin. Invest. 119, 1420-1428). These EMTs are observed in embryonic cell layer movement and organogenesis during normal embryonic development, but in the 1990s EMT began to be reported as a pathophysiological cause mediating tumor malignancy and chronic fibrosis processes (Flanders, KC (2004)). Int. J. Exp. Pathol. 85, 47-64). EMT specifically plays a role in tumor invasion, intravasation and extravasation of metastatic cancer cells, and in the case of chronic fibrosis, EMT plays an important role in the production of fibroblasts that induce extracellular matrix accumulation. (Thiery, JP, et al. (2009). Cell 139, 871-890; Iwatsuki, M., et al. (2009) Epithelial-mesenchymal transition in cancer development and its clinical significance.Cancer Sci. (Equb ahead of print) )).

The most important hallmark of EMT is the transformation of cell-cell junctions (tight junctions, desmosomes, adherens junctions, gap junctions) between epithelial cells, resulting in disruption of epithelial polarity and rearrangement of actin cytoskeleton. This change in cytoskeleton promotes cell migration and induces spindle-shaped mesenchymal-derived cells. In this process, the expression of epidermal marker proteins E-cadherin and Zeosin was decreased, and the expression of Snail, ZEB1, and N-cadherin, markers of mesenchymal-derived cells, was increased (Evdokimova, V., et al. Cancer Cell 15, 402-415; Huber, MA, Kraut, N., and Beug, H. (2005), Curr. Opin. Cell Biol. 17, 548-558). Therefore, understanding at the molecular level of the EMT phenomenon, which is one of the characteristics of cancer cells during malignancy, can broaden the understanding of EMT-related diseases and contribute greatly to the discovery of targets for artificially modulating EMT.

And _{includes, hydrogen peroxide (H 2 O 2} ), hydroxyl anion (· OH) such as ^- active oxygen species (reactive oxygen species, ROS) is mainly produced as a by-product of the aerobic organism breathing, superoxide anion (O2 ·). However, recent studies have shown that intracellular ROS levels are a very important physiological factor and many phenomena depend on ROS. Low concentrations of ROS, for example, play an important role as secondary messengers in cell growth hormone-induced cell proliferation and induce antioxidant enzyme activity (Wang, J. and Yi, J. (2008). Cancer Biol. Ther. 7, 1875-1884; Wu, WS (2006). Cancer Metastasis Rev. 25, 695-705). In the case of cancer cells, ROS production level is estimated to be higher than normal cells due to active energy consumption, which is expected to contribute greatly to cancer cell survival through cancer cell proliferation and induction of antioxidant enzyme activity. However, there is a lack of understanding of the source, role and mechanism of action of these ROS in the proliferation, survival and metastasis of cancer cells.

In non-phagocytic animal cells, reactive oxygen species are produced at low concentrations by the NADPH oxidase complex (hereinafter referred to as 'Nox'). In animal cells, the Nox family consists of Nox1, Nox2, Nox3, Nox4, Nox5, DUOX1 and DUOX2 proteins (Clrkin, JS, Naughton, R., Quiney, C., and Cotter, TG (2008) Cancer Letters 266 , 30-36). The low concentrations of free radicals produced by these Nox systems act as a second messenger in cells and act on various cellular signaling targets and cell cycle targets.

The present invention solves the above problems, and it is an object of the present invention to provide a protein for cancer diagnosis.

Another object of the present invention is to provide a gene for cancer diagnosis.

Still another object of the present invention is to provide a substrate to which a protein for diagnosis of cancer is immobilized.

Still another object of the present invention is to provide a substrate in which a gene for diagnosis of cancer is immobilized.

Still another object of the present invention is to provide a composition for preventing and / or treating cancer.

In order to achieve the above object, the present invention provides a polypeptide selected from the group consisting of NADPH oxidase complex (Nox) 1, NADPH oxidase complex (Nox) 4 and NADPH oxidase complex (Nox) 5 polypeptides and functional variants thereof as an active ingredient. It provides a cancer diagnostic composition comprising.

The term "polypeptide" described in the specification of the present invention refers to the entire length of the polypeptide according to the present invention. In a preferred embodiment, the term “polypeptide” includes polypeptides produced by isolated polypeptides and recombinant methods, such as by separating and purifying from a sample, by screening libraries and by protein synthesis by conventional methods. All of the above methods are known to those skilled in the art. Preferably, the entire polypeptide, or a portion thereof, can be synthesized by conventional synthetic methods, such as the Merrifield technique. In another preferred embodiment, some of the polypeptides according to the invention can be used for screening suitable gene libraries prepared to express encoded protein sequences to identify functional variants of the polypeptides according to the invention. It can be used to obtain monoclonal antibodies.

NADPH Oxidase Complex (Nox 1), NADPH Oxidase Complex (Nox) 4, and NADPH Oxidase Complex (Nox) 5 polypeptide sequences of the present invention refer to polypeptides having the amino acid sequences set forth in SEQ ID NOs: 19, 20-21.

A “functional variant” of a polypeptide described herein is sequence homologous, in particular about 70%, preferably about 80%, in particular about 90%, relative to a polypeptide having an amino acid sequence according to one of SEQ ID NOs: 19-21. In particular it refers to a polypeptide having about 95%, most preferably about 98% sequence identity. Such functional variants are polypeptides which are homologous to the polypeptides according to the invention, for example, originating in organisms other than humans, preferably from non-human mammals such as mice, rats, monkeys and pigs. Another example of a functional variant is a polypeptide encoded by different alleles of genes in different individuals, different organs of one organism or at different stages of development. Functional variants preferably also include naturally occurring or synthetic mutations, especially those which significantly alter the activity of peptides encoded by these sequences. Such variants may also result from different conjugation of genes that encode, preferably.

"Functional variant" refers to a polypeptide having the same biological activity as the corresponding polypeptide according to the invention. Such biological actions can be analyzed by functional assays. To examine whether a candidate polypeptide is a functional variant of a polypeptide according to the present invention, the candidate polypeptide can be analyzed by functional assays generally known to those skilled in the art. Such assays are suitable for analyzing the biological function of the corresponding polypeptides according to the invention. Such functional assays include cell culture systems; Generation of a mouse that has deleted a gene (“knock out”) or a transgenic mouse for a gene encoding a candidate polypeptide; Enzymatic assays and the like. If the candidate polypeptide exhibits or directly interferes with a biological function that is substantially the same as the corresponding polypeptide according to the present invention, then the candidate polypeptide may be used as long as the candidate polypeptide meets the requirements for the level of% sequence identity described above. It is a functional variant.

The term “functional variant” also refers to a reference sample, including a polypeptide expressed from a mutated gene or from a differentially conjugated gene so long as the candidate functional variant polypeptide meets the criteria for a functional variant for% sequence identity level or Reference libraries include polypeptides differentially expressed in patients with breast cancer. Such expression analysis can be carried out by methods generally known to those skilled in the art.

"Functional variants" of polypeptides range from about 7 to about 1000 amino acids, preferably 10 or more amino acids, more preferably 20 or more, most so long as they have substantially the same biological function as the corresponding polypeptide according to the invention. Preferably at least 50, such as at least 100, such as at least 200, such as at least 300, such as at least 400, such as at least 500, such as at least 600 amino acids, may be part of a polypeptide according to the invention. Also included are deletions of polypeptides according to the invention in the range of about 1 to 30, preferably about 1 to 15, especially about 1 to 5 amino acids, so long as they have substantially the same biological function as the corresponding polypeptides according to the invention. do. For example, the first amino acid methionine may not be present without significantly altering the function of the polypeptide. In addition, post-translational modifications such as lipid anchor or phosphoryl groups may or may not be present in the variant.

"Sequence identity" refers to a polypeptide as determined by BLASTP 2.0.1 and a nucleic acid as determined by BLASTN 2.014 (where a filter is installed and BLOSUM is 62; Altschul et al., 1997, Nucleic Acids Res. , 25: 3389-3402), to the degree of identity (% identity) of the two sequences.

"Sequence homology" refers to the similarity of two polypeptide sequences determined by BLASTP 2.0.1 (where filters are installed and BLOSUM is 62; Altschul et al., 1997, Nucleic Acids Res., 25: 3389-3402) % Positive).

In addition, the present invention includes a gene selected from the group consisting of NADPH oxidase complex (Nox) 1, NADPH oxidase complex (Nox) 4 and NADPH oxidase complex (Nox) 5 polypeptide and functional variants thereof as an active ingredient. It provides a composition for diagnosing cancer.

The genes encoding the NADPH Oxidase Complex (Nox) 1, the NADPH Oxidase Complex (Nox) 4, and the NADPH Oxidase Complex (Nox) 5 polypeptides described in the specification of the present invention are genes according to SEQ ID NOs: 22 to 24 and / or Refers to a variant.

The term “coding gene” relates to a DNA sequence or a precursor thereof that encodes a separable bioactive polypeptide according to the invention. Polypeptides may be encoded by sequences of the entire length or portion thereof, as long as the biological function, such as receptor-activity is substantially maintained (see definition of functional variant).

In the sequences of genes described above, for example, due to the degeneracy of the genetic code, there may be little change or an untranslated sequence may be attached to the 5 'and / or 3' ends of the nucleic acid without significantly affecting the activity of the encoded polypeptide. It is known that there is. The present invention therefore also encompasses so-called naturally occurring nucleic acids and artificially generated "variants" of the nucleic acids described above.

Preferably, the gene according to the invention is DNA or RNA, preferably DNA, in particular double stranded DNA. In particular the gene according to the invention may be an RNA molecule, preferably a single or double chain RNA molecule. The sequence of nucleic acids may further comprise one or more introns and / or one polyA sequence.

The genes according to the invention can be prepared by methods generally known to those skilled in the art and are described below.

Within the meaning of the present invention "variant" refers to any DNA sequence that is complementary to a DNA sequence that hybridizes with a reference sequence under stringent conditions and has similar activity to a polypeptide according to the present invention. The genes according to the invention can also be used in the form of antisense sequences.

The “variant” of a gene may be a homologue obtained from another species having sequence identity of preferably 80%, in particular 90%, most preferably 95%.

A “variant” of a gene is at least about 8 nucleotides in length, preferably at least about 16 nucleotides in length, in particular at least about 21 nucleotides in length, more preferably about 30, as long as it has similar activity as the corresponding polypeptide according to the invention. It may be part of a gene according to the invention having at least about nucleotides in length, more preferably at least about 40 nucleotides in length and most preferably at least about 50 nucleotides in length. Such activity can be assayed using the functional assays described above.

As a preferred embodiment of the invention, the gene may comprise a gene having a sequence complementary to the gene according to the invention or a variant thereof. Preferably said gene comprises non-functional mutagenic variants of the genes according to the invention, or variants thereof.

The present invention also provides a substrate for diagnosing cancer to which a protein selected from the group consisting of NADPH oxidase complex (Nox) 1, NADPH oxidase complex (Nox) 4 and NADPH oxidase complex (Nox) 5 polypeptides and functional variants thereof is attached. .

In addition, the present invention is for diagnosing cancer with a gene selected from the group consisting of genes encoding NADPH oxidase complexes (Nox) 1, NADPH oxidase complexes (Nox) 4 and NADPH oxidase complexes (Nox) 5 polypeptides and functional variants thereof. Provide the substrate.

In one embodiment of the invention, the substrate is preferably nickel-PTFE (polytetrafluoroethylene), glass, apatite, silicon, gold, silver or metal, but is not limited thereto.

The protein or gene of the present invention can be immobilized on the substrate by a method known per se. For example, the method of introducing an affinity substance or predetermined functional group into the protein or gene of this invention, and then immobilizing to a substrate using this affinity substance or predetermined functional group is mentioned. A predetermined functional group may be a functional group which can be provided to a coupling reaction, and an amino group, a thiol group, a hydroxyl group, a carboxyl group, and an aldehyde group are mentioned, for example.

The present invention also provides a composition for preventing and treating cancer, which contains a therapeutically effective amount of diphenylene iodonium, its derivatives, or a pharmaceutically acceptable salt thereof as an active ingredient.

“Treating”, “treatment” or “prevention” as described herein refers to both therapeutic treatment and preventive or preventive measures, the aim of which is to prevent or alleviate (reduce) targeted pathological symptoms or disorders. to be. Subjects in need of treatment include those already suffering from the disorder, as well as those susceptible to the disorder or those in which the disorder is to be prevented. Reduction in the number of cancer cells or absence of cancer cells after administration of a therapeutic amount of diphenyleneiodonium in accordance with the methods of the invention; Reduction in tumor size; Inhibition (ie, slowing to some extent and preferably stopping) of cancer cell infiltration into peripheral organs, including metastasis of cancer to soft tissues and bones; Inhibit (ie, slow to some extent and preferably stop) tumor metastasis; Inhibition to some extent of tumor growth; And / or relieve to some extent one or more of the symptoms associated with the particular cancer; Reduced morbidity and mortality; And when one or more of the improvement in quality of life is reduced or does not appear to the extent observable and / or measurable in the patient when the subject or mammal has been successfully “treated” for cancer. Diphenylene iodonium and its derivatives may exhibit cell arrest and / or cytotoxicity when they are capable of disrupting the growth of existing cancer cells and / or killing existing cancer cells. Reduction of these signs or symptoms may be felt by the patient.

The above parameters for evaluating successful treatment and improvement of the disease can be easily measured using conventional procedures known to the physician. For cancer therapy, efficacy can be determined, for example, by evaluating the time spent on disease progression (TTP) and / or measuring the response rate (RR). Metastasis can be determined through disease stage determination tests and bone scans and tests of calcium levels and other enzymes to determine whether cancer has metastasized to bone. CT scans can also be done to look for metastases to the pelvis and lymph nodes. Chest X-rays and measurements of liver enzyme levels by known methods are used to determine whether they have metastasized to the lung and liver, respectively. Other conventional methods for monitoring the disease include transrectal ultrasonography (TRUS) and transrectal saliva biopsy (TRNB).

"Long-term" administration refers to the administration of the agent (s) in a continuous manner as opposed to a short-term administration so that the initial therapeutic effect (activity) is maintained for an extended period of time. "Intermittent" administration is a therapy characterized by periodic administration, not continuation, without interruption.

For the purpose of treating cancer, alleviating the symptoms of cancer, or diagnosing cancer, a "mammal" is used for humans, livestock and animal husbandry, zoo animals, competition animals or pets such as dogs. And any animal classified as a mammal, including cat, cow, horse, sheep, pig, goat, rabbit, and the like. Preferably, the mammal is a human.

In addition, the diphenylene iodonium, derivatives thereof, or pharmaceutically acceptable salts thereof of the present invention are cisplatin, taxol, taxotere, doxorubicin, etoposide. , Gleevec, Arimidex, Tamoxifen, Epirubicin, Adriamycin, Mitomycin-C, Flutamide, Josperin ( Gosereline, Gemcitabine, Megesterol acetate, Mesotrexate, Methatrexate, Dacarbazine, Amsacrine, Fluorouracil, Ifosfamide, Melphalan, Tyotepa, Carboplatin, Chlorambuci, Cyclophosphamide, Vinblastine, Hydroblasturea, Na Belvin, Gemzar, and Campobe ll, Rufron, Eulexin, Zometa, Hycamtin, and Valstar in combination with at least one anticancer agent selected from, but not limited to No.

“Combination” administration with one or more additional therapeutic agents includes administration at the same time (in combination) and administration in any order sequentially.

The term "therapeutically effective amount" refers to an amount of diphenyleneiodonium or other drug effective for "treatment" of a disease or disorder in a subject or mammal. In the case of cancer, the therapeutically effective amount of the drug may be a reduction in the number of cancer cells; Reduction in tumor size; Inhibit (ie, slow to some extent and preferably stop) cancer cell infiltration into peripheral organs; Inhibit (ie, slow to some extent and preferably stop) tumor metastasis; Inhibition to some extent of tumor growth; And / or relieve to some extent one or more of the symptoms associated with cancer. See the definition for “treatment” herein. Drugs may exhibit cell arrest and / or cytotoxicity when they are capable of disrupting the growth of existing cancer cells and / or killing existing cancer cells.

As used herein, "carrier" includes pharmaceutically acceptable carriers, excipients or stabilizers, which are nontoxic to cells or mammals exposed to them at the dosages and concentrations employed. Often, the physiologically acceptable carrier is an aqueous pH buffer solution. Examples of physiologically acceptable carriers include buffers such as phosphate, citrate and other organic acids; Antioxidants including ascorbic acid; Low molecular weight (less than about 10 residues) polypeptides; Proteins such as serum albumin, gelatin or immunoglobulins; Hydrophilic polymers such as polyvinylpyrrolidone; Amino acids such as glycine, glutamine, asparagine, arginine or lysine; Monosaccharides, disaccharides, and other carbohydrates including glucose, mannose, or dextrins; Chelating agents such as EDTA; Sugar alcohols such as mannitol or sorbitol; Salt-forming counter ions such as sodium; And / or nonionic surfactants such as TWEEN®, polyethylene glycol (PEG) and PLURONICS®.

As used herein, the term "cancer diagnosis" refers to one in a subject, including, but not limited to, the presence of benign tissue, pre-cancerous tissue or cancer tissue, stage of cancer, and prognosis of the subject, and the like. The above means the confirmation of the characteristics of the cancer sample. Cancer may be characterized by identifying expression of one or more cancer markers, including but not limited to cancer marker proteins described herein.

As used herein, the term “detecting reduced or increased expression for non-cancer controls” refers to measuring gene expression levels (eg, levels of mRNA or protein) compared to levels in non-cancer samples. To mention. Gene expression can be measured using any suitable method, including but not limited to those described herein.

As used herein, the term “patient diagnosed with cancer” refers to a subject that has been tested and found to have cancer cells. Cancer can be diagnosed by any suitable method, including but not limited to biopsy, X-rays, blood tests, and diagnostic methods according to the invention.

As used herein, the term “biopsy tissue” refers to a sample of tissue removed from a subject to determine whether the sample comprises cancer tissue. In one embodiment, biopsy tissue is obtained because the subject is suspected of having cancer. The biopsy tissue is then tested for the presence or absence of cancer.

As used herein, the term “vector” is used for nucleic acid molecules that deliver DNA fragments from one cell to another. The term "vehicle" is sometimes used interchangeably with "vector". Vectors are often derived from plasmids, bacteriophages or plant or animal viruses.

As used herein, the term “expression vector” refers to a recombinant DNA comprising a suitable nucleic acid sequence necessary for the expression of the coding sequence of interest and the coding sequence operably linked in a particular host organism. Nucleic acid sequences required for expression in prokaryotic cells generally, along with other sequences, include promoters, operators (optional), and ribosomal binding sites. Eukaryotic cells are known to utilize promoters, enhancers, and termination and polyadenylation signals.

The terms "overexpression" and "overexpressing" and grammatical equivalents are used for levels of mRNA to indicate expression levels of at least about 1.5-fold (or more) than those observed in designated tissues in control non-transgenic animals. . mRNA levels are measured using many techniques known to those of skill in the art, including but not limited to Northern blot analysis. Suitable controls are included in the Northern blot as a control for the difference in the amount of RNA loaded from each tissue to be analyzed.

As used herein, the term "in vitro" refers to an artificial environment and processes or reactions that occur in an artificial environment. The in vitro environment may consist of, but is not limited to, test tubes and cell cultures. The term "in vivo" refers to a natural environment (eg, an animal or a cell) and a process or reaction that occurs in the natural environment.

As used herein, the term "sample" is used in a broad sense. In one sense, it is meant to include specimens or cultures obtained from all sources, and biological and environmental samples. Biological samples may be obtained from animals (including humans) and may include latex, solids, tissues, and gases. Biological samples include blood products such as plasma, serum, and the like. Environmental samples include environmental materials such as surface materials, soil, water, crystals and industrial samples.

As used herein, the terms "cancer" and "tumor" refer to or describe a pathological condition in mammals that is typically characterized by unregulated cell growth. Examples of cancer include, but are not limited to, carcinoma, lymphoma, blastoma, sarcoma, and leukemia. More specific examples of such cancers include squamous cell carcinoma, small cell lung cancer, non-small cell lung cancer, lung adenocarcinoma, lung squamous carcinoma, peritoneal cancer, hepatocellular cancer, gastrointestinal cancer, pancreatic cancer, glioblastoma, Cervical cancer, ovarian cancer, liver cancer, bladder cancer, hepatoma, breast cancer, colon cancer, colon cancer, endometrial cancer or uterine cancer, salivary gland cancer, kidney cancer, prostate cancer, vulvar cancer, thyroid cancer, hepatocarcinoma (hepatic cancer) carcinoma) and various types of head and neck cancers.

The present invention can be used in connection with any method for measuring gene expression patterns of related cells as well as proteins, based on gene expression measurement methods. Preferred methods for establishing gene expression profiles include measuring the amount of RNA produced by a gene capable of encoding a protein or peptide. This is done by reverse transcriptase PCR (RT-PCR), competitive RT-PCR, real time RT-PCR, differential RT-PCR, Northern blot analysis and other related tests. Although such techniques using individual PCR reactions can be used, it is best to amplify the copy DNA (cDNA) or copy RNA (cRNA) generated from mRNA and then analyze it via microarray. Many different microarray forms and methods for their preparation are known to those skilled in the art and are described in US Pat. Nos. 5,445,934, 5,532,128, 5,556,752, 5,242,974, 5,384,261, 5,405,783, 5,412,087, 5,424,186, 5,429,807, 5,436,327, 5,472,672, 5,527,681, 5,529,756, 5,545,531, 5,554,501, 5,561,5,561,071,5,539,557,557 , 5,599,695, 5,624,711, 5,658,734, and 5,700,637.

Microarray technology can provide a powerful means of identifying effects such as initiation, arrest, and regulation of unregulated cell proliferation by simultaneously measuring steady-state mRNA levels of thousands of genes. Two microarray technologies are currently in wide use. The first is cDNA microarray and the second is oligonucleotide microarray. Although there are differences in the structure of these chips, all underlying data analysis and output are essentially the same. The result of this analysis is a measure of the intensity of a signal received from a labeled probe that is typically used to detect a cDNA sequence of a sample hybridized with a nucleic acid sequence at a known position in a microarray. Typically, the strength of the signal is proportional to the amount of cDNA and thus proportional to the amount of mRNA expressed in the sample cell. Many of these techniques are available and useful. Preferred methods for measuring gene expression are U.S. Pat.Nos. 6,271,002 (Linsley et al.), 6,218,122 (Friend et al.), 6,218,114 (Peck et al.) And 6,004,755 (Wang et al., Incorporated herein by reference). ).

Analysis of expression levels is performed by comparing the above-described intensities. It is best to calculate a ratio matrix of the expression intensity of the gene in the test sample to the expression intensity of the gene in the control sample. For example, the gene expression intensity of diseased tissue can be compared with the expression intensity generated from the same type of normal tissue (eg, diseased colon tissue sample and normal colon tissue sample). The ratio of this expression intensity is expressed as a fold change in gene expression of test and control samples.

Abnormal cells have regulated genes that are specifically expressed, such as upregulation or downregulation. Upregulation and downregulation are relative terms meaning that detectable differences are found in the expression level of the gene (overcoming the noise contribution of the system used to measure it) relative to the same criteria. In this case, the reference is a measure of gene expression in normal cells. Thus, genes in question of abnormal cells are up- or down-regulated compared to baseline levels using the same measurement method.

Preferably the level of up and down regulation is distinguished based on a fold change in the intensity measurement of the hybridized microarray probe. For example, when a difference of 1.5 times or more is used for such a distinction, it can be seen that diseased cells exhibit 1.5 or more or less than 1.5 times intensity compared to normal cells.

Another way is to distinguish them. For example, statistical assays can be used to find the most significantly different genes between various sample groups. For example, the Student t-test is a robust statistical method that can be used to detect significant differences between the two groups. Smaller p values provide stronger evidence that the genes differ between different groups. Nevertheless, because microarrays measure more than one gene at the same time, tens of thousands of statistical assays may be required at one time. For this reason, it is possible to obtain small p-values by varying and adjusting using the Sidak correction as well as random / substitution experiments.

The present invention is based, in part, on the dosage regimen of oral dosage compositions comprising diphenyleneiodonium, derivatives thereof, or pharmaceutically acceptable salts thereof. In addition, it is based, in part, on the therapeutic efficacy of the diphenyleneiodonium, derivatives thereof, or pharmaceutically acceptable salts thereof of the present invention on certain cancers.

The present invention includes a method of treating a tumor of a mammal, which relates to administering to the mammal a therapeutically effective amount of diphenylene iodonium, a derivative thereof, or a pharmaceutically acceptable salt thereof. Or in combination with one or more therapeutic agents to a mammal in need of such treatment.

In the specification of the present invention, the term "derivative" is the broadest meaning, and when the organic compound is considered as a matrix, the structure and properties of the mother, such as introduction of functional groups, oxidation, reduction, substitution of atoms, etc., are changed as long as they do not significantly change. Say a compound. Accordingly, the present invention is directed to the introduction, oxidation, reduction, atom substitution, etc. of a functional group based on a diphenylene iodonium compound having a similar activity to a diphenylene iodonium compound in the prevention and / or treatment of a specific cancer. It includes all derivatives that are easily recognized by those skilled in the art, specific examples thereof include Dibenziodium, chloride (= Diphenylenpropodium iodide); Dibenziodium, sulfate; Dibenziodolium, 3-chloro-, butanoate; Dibenziodolium, 3-chloro-, carbonate; Dibenziodolium, 3-chloro-, 2-hydroxy-1,2,3-propanetricarboxylate (3-); Dibenziodolium, 3-chloro-, cyclopropanecarboxylate; Dibenziodolium, 3-chloro-, hydroxide; Dibenziodolium, 3-chloro-, 2-hydroxyethanesulfonate; Dibenziodolium, 2-chloro-, malonate (7Cl); Dibenziodolium, 3-chloro-, methanesulfonate; Dibenziodolium, 3-chloro-, propanoate; Dibenziodolium, 3-amino-, chloride; Dibenziodolium, 1-bromo-3,7-dinitro-, chloride; Dibenziodolium, 3,7-dichloro-, chloride; Dibenziodolium, 3,7-diamino-, chloride; 5H-Dibenziodole, 5- (3-chlorophenyl)-(9Cl); 5H-Dibenziodole, 5- (4-chlorophenyl)-(9Cl); Phenanthro [4,5-bcd ] iodolium, chloride (8Cl); Dibenziodolium, 1-iodo-, chloride; Dibenziodolium, 2-chloro-, phosphate; Dibenziodolium, salt with 2,4,5-trichlorophenol (8Cl); Dibenziodolium, 3-chloro-, sulfate; Dibenziodolium, 3,7-dichloro-, sulfate; Dibenziodolium, 2-chloro-, sulfate; Dibenziodolium, 2,4- dichloro-, sulfate; Dibenziodolium, 3,7-dinitro-, chloride; Dibenziodolium, 1- (dimethylamino) -2-naphthalenesulfonate; Dibenziodolium, salt with 4-[[(trifluoromethyl) sulfonyl] oxy] benzenesulfonic acid; Dibenziodolium-2,4,6,8 -t4 (9Cl); Dibenziodolium, salt with chromic acid (H2Cr2O7) (9Cl); Dibenziodolium, (OC-6-11) -hexafluoroantimonate (1-); Dibenziodolium, salt with trifluoroacetic acid (9Cl); Dibenziodolium, salt with trifluoromethanesulfonic acid (9Cl); Dibenziodolium, salt with 9,10-dihydro-9,10-dioxo-2-anthracenesulfonic acid (9Cl); Dibenziodolium, salt with 9,10-dimethoxy-2-anthracenesulfonic acid (9Cl); Dibenziodolium, O -ethyl carbonodithioate (9Cl); Dibenziodolium, salt with 1,1 '-[[(methylsulfonyl) methylene] bis (sulfonyl)] bis [benzene] (9Cl); Dibenziodolium, diethylcarbamodithioate (9Cl); Dibenziodonium, 1-pyrrolidinecarbodithioate, compd . with chloromethane (9Cl); Dibenziodolium, 1-pyrrolidinecarbodithioate, compd. with trichloromethane (9Cl); Dibenziodolium, 1-pyrrolidinecarbodithioate (9Cl); 5H-Dibenziodole, 5- (2-naphthylthio)-(7Cl); 1,3-Cyclohexanedione, 5,5-dimethyl-, 5H-dibenziodole deriv. (7Cl); Dibenziodolium, (T-4) -tetraiodomercurate (2-) (9Cl); Dibenziodolium-131I (9Cl); Dibenziodolium, salt with 4-methylbenzenesulfonic acid (9Cl); Dibenziodolium, hexafluoroarsenate (1-) (9Cl) ; Dibenziodolium, bromide; Dibenziodolium-5-125I (9Cl); Dibenziodolium, trichloromercurate (1-) (8Cl); Dibenziodolium, iodate (8Cl); Dibenziodolium, tetrafluoroborate (1-) (8Cl, 9Cl); Dibenziodolium, hexafluorophosphate (1 -); Dibenziodolium, citrate (8Cl); Dibenziodolium, salt with 2-hydroxyethanesulfonic acid (8Cl); Dibenziodolium, methanesulfonate (7Cl, 8Cl, 9Cl); Dibenziodolium, 2-hydroxyethyl sulfate (9Cl); Dibenziodolium, methyl sulfate (8Cl); Dibenziodolium, oxonium; Dibenziodolium, cyclopropanecarboxylate (7Cl, 8Cl); Dibenziodolium, butyrate (7Cl, 8Cl); Dibenziodolium, propionate (7Cl, 8Cl); Dibenziodolium, carbonate (8Cl, 9Cl); Dibenziodolium, sulfate (8Cl, 9Cl); Dibenziodolium, salt with 2,4,5-trichlorophenol (8Cl); Dibenziodolium, acetate (7Cl, 8Cl); Dibenziodolium, phosphate (8Cl); Dibenziodolium , glycolate (7Cl, 8Cl); Dibenziodolium, salt with 2-hydroxypropanoic acid (9Cl); Dibenziodolium, nitrate (6Cl, 7Cl, 8Cl, 9Cl); Dibenziodolium, iodide; Dibenziodolium, fluoride

5H-Dibenziodole (8Cl, 9Cl); Dibenziodolium, 3-[[2-[(2-aminoethyl) thio] acetyl] amino]-; Dibenziodolium, 3-[[2-[(2-aminoethyl) amino] acetyl] amino ]-; Dibenziodolium, 3-[[2-[(2-aminoethyl) thio] acetyl] amino]-, chloride, hydrochloride; Dibenziodolium, 3-[[2-[(2-aminoethyl) amino] acetyl] amino]- Dibenziodolium, 3-nitro-, chloride (1: 1); Dibenziodolium, 3,7-dinitro-, tetrakis (2,3,4,5,6-pentafluorophenyl) borate (1-); Dibenziodolium, 3-nitro-, tetrakis (2,3,4,5,6-pentafluorophenyl) borate (1-); Dibenziodolium, 3-nitro-, tetraphenylborate (1-); Dibenziodolium, 1- (dimethylamino) -2-naphthalenesulfonate; Dinaphth [1,2-b: 1 ', 2'-d] iodolium, salt with trifluoromethanesulfonic acid; Dinaphth [1,2-b: 1', 2'-d] iodolium, tetrakis [3,5-bis (trifluoromethyl ) phenyl] borate (1-) (9Cl); Dinaphth [2,1-b: 1 ', 2'-d] iodolium (9Cl); Dibenziodolium, 3-chloro-, butanoate; Dibenziodole, 1-methyl-, ion (1-); Dinaphth [2,3-b: 2 ', 3'-d] iodolium, 1,2,3,4,8,9,10,11-octahydro; Dibenziodolium, 1-methoxy-; Dibenziodolium, 1,9-dimethoxy; Dib enziodolium, 3-methoxy; Dibenziodolium, 1,2,3,7,8,9-hexamethoxy; Dibenziodolium, 3,7-bis (methoxycarbonyl); Dibenziodolium, 1- (1,3-dihydro-1,3-dioxo- 2H-isoindol-2-yl)-; Dibenziodolium, 3,7-bis (trifluoromethyl); Dibenziodolium, 1-iodo; Dibenziodolium, 1-nitro; Dibenziodolium, 3-methyl; Dibenziodolium, 3,7-dibromo; Dibenziodolium, 3 -chloro-, carbonate; Dibenziodolium, 3-chloro-, 2-hydroxy-1,2,3-propanetricarboxylate (3-); Dibenziodolium, 3-chloro-, cyclopropanecarboxylate; Dibenziodolium, 3-chloro-, hydroxide; Dibenziodolium, 3-chloro-, 2- hydroxyethanesulfonate; Dibenziodolium, 2-chloro-, malonate (7Cl); Dibenziodolium, 3-chloro-, methanesulfonate;

Dibenziodolium, 3-chloro-, propanoate; Dibenziodolium, 3,7-diiodo; Dibenziodolium, 1,2,3,4-tetramethyl; Dibenziodolium, 3,7-diamino; 1,20: 3,6-Dimetheno-6H, 8H , 20H- [1,10] dioxacycloheneicosino [5,6-c] iodolium; 9,10,11,12,13,14,15,16,17,18-decahydro- (9Cl); Dibenziodolium, 2,3- dimethoxy; Dibenziodolium, 2,7-dinitro; 1,20: 3,6-Dimetheno-6H, 20H- [1,4,7,10,13] pentaoxacycloheneicosino [17,18-c] iodolium, 8,9,11,12,14,15,17,18 -octahydro- (9Cl); Dibenziodolium, 1-phenyl; Dibenziodolium, 3,7-bis (trimethylammonio)-; Dibenziodolium, 1,4-dimethyl; Dibenziodolium, 1-bromo-3,7-dinitro; Dibenziodolium, salt with 4 -[[(trifluoromethyl) sulfonyl] oxy] benzenesulfonic acid (9Cl); Dibenziodolium, 3-amino; 1,20: 3,6-Dimetheno-6H, 8H, 20H- [1,10] dioxacycloheneicosino [5,6-c] iodolium (9Cl); 1,20: 3,6-Dimetheno-6H, 20H- [1 , 4,7,10,13] pentaoxacycloheneicosino [17,18-c] iodolium (9Cl); 1,20: 3,6-Dimetheno-6H, 8H, 20H- [1,10] dioxacycloheneicosino [5,6-c ] iodolium, 9,10,11,12,13,14,15,16,17,18-decahydro-, iodide (9Cl); 1,20: 3,6-Dimetheno-6H, 20H- [1,4, 7,10,13] pentaoxacycloheneicosino [17,18-c] iodolium, 8,9,11,12,14,15,17,18-octahydro-, iodide (9Cl); Dibenziodolium-2,4,6,8- t4, 3-nitro- (9Cl); Dibenziodolium-2,4,6,8-t4 (9Cl); Dinaphth [1,2-b: 1 ', 2'-d] iodolium, (13bS)-, tetrafluoroborate ( 1-) (9Cl); Dinaphth [1,2-b: 1 ', 2'-d] iodolium, (13bS)-(9Cl); 5H-Dibenziodol-5-yl, 3- (nitro-15N)-( 9Cl); 5H-Dibenziodol-5-yl, 3-nitro- (9Cl); Dibenziodolium, 3-nitro-, sulfate; Dibenziodolium, 3-amino-, chloride; Dibenziodolium, 3,3'-methylenebis-, salt with 9 , 10-dimethoxy-2-anthracenesulfonic acid (9Cl); Dibenziodolium, 3,3'-methylenebis- (9Cl); Dibenziodolium, salt with chromic acid (H ₂ Cr ₂ O ₇ ) (9Cl); Dibenziodolium, 1-methyl- , sulfate; Dibenziod olium, 1-methyl; Dibenziodolium, 1-methyl-, chloride; Dibenziodolium, 3-methyl-, chloride; Dibenziodolium, 1-bromo-3,7-dinitro-, iodide; Dibenziodolium, 1-bromo-3,7-dinitro -, bromide; Dibenziodolium, 1-bromo-3,7-dinitro-, chloride; Dibenziodolium, (OC-6-11) -hexafluoroantimonate (1-); Dibenziodolium, salt with trifluoroacetic acid (9Cl); Dibenziodolium, salt with trifluoromethanesulfonic acid (9Cl); Dibenziodolium, 3,7-bis (1,1-dimethylethyl)-, 1,1,1-trifluoromethanesulfonate; Dibenziodolium, 3,7-bis (1,1-dimethylethyl)-; Dibenziodolium, 2-chloro -, tetrafluoroborate (1-); 5H-Dibenziodole, 5-[(methylsulfonyl) bis (phenylsulfonyl) methyl]-(9Cl); Dibenziodolium, salt with 9,10-dihydro-9,10-dioxo-2-anthracenesulfonic acid ( 9Cl); Dibenziodolium, salt with 9,10-dimethoxy-2-anthracenesulfonic acid (9Cl); Dibenziodolium, 3,7-bis (dimethylamino) -4,6-bis (hydroxymethyl)-, inner salt; Dibenziodolium, O-ethyl carbonodithioate (9Cl Dibenziodolium, salt with 1,1 '-[[(methylsulfonyl) methylene] bis (sulfonyl)] bis [benzene] (9Cl);

Dibenziodolium, diethylcarbamodithioate (9Cl); Dinaphth [2,3-b: 2 ', 3'-d] iodolium, 1,2,3,4,8,9,10,11-octahydro-, iodide; Dinaphth [2, 3-b: 2 ', 3'-d] iodolium, iodide; Dibenziodolium, 1,9-dimethoxy-, salt with iodonium; Dibenziodolium, 1-methoxy-, iodide; 1-Methyldibenziodolium iodide (6Cl); Dibenziodolium, 3- methoxy-, iodide; Dibenziodolium, 2-bromo-, iodide; Dibenziodolium, 2,7-dinitro-, iodide; Dibenziodolium, 3-nitro-, iodide; Dibenziodolium, 3-nitro-, nitrate; Dibenziodonium, 1-pyrrolidinecarbodithioate, compd . with chloromethane (9Cl); Dibenziodolium, 1-pyrrolidinecarbodithioate, compd. with trichloromethane (9Cl); Dibenziodolium, 1-pyrrolidinecarbodithioate (9Cl); 5H-Dibenziodole, 5- (2-naphthylthio)-(7Cl); Dibenziodolium, 1,2,3,7,8,9-hexamethoxy-, iodide; 1,3-Cyclohexanedione, 5,5-dimethyl-, 5H-dibenziodole deriv. (7Cl); Dibenziodolium, 3,7-bis (diethylamino)-, iodide; Dibenziodolium, 3,7-bis (ethylmethylamino)-, formate, including but not limited to.

The compositions of the present invention may be used in accordance with the present invention in any suitable route of administration, including intravenous and oral administration, including intravenous, subcutaneous, intramuscular and intradural and intranasal, rectal or vaginal. However, the present invention is not limited thereto. It is also administered directly into the tumor, via a transdermal patch, or via an insertion device (especially sustained release). The pharmaceutical compositions can be used in the form of sterile physiologically acceptable (aqueous or organic) solutions, colloids, suspensions, creams, ointments, pastes, caplets, tablets and cachets. This case also includes sustained release or delayed release dosage forms.

1.Pharmaceutical Formulations

The present invention relates to a pharmaceutical anticancer composition comprising diphenylene iodonium, a derivative thereof, in a pharmaceutically effective amount, comprising one or more pharmaceutically acceptable adjuvants, excipients, carriers, buffers, diluents and / or conventional pharmaceuticals It relates to a pharmaceutically acceptable salt thereof for the preparation of adjuvants. In an embodiment of the present invention, the compound of the present invention may be administered in a pharmaceutically acceptable formulation. The present invention is a synthetic formulation in the form of lipid-based formulations and macromolecular complexes, nanocapsules and microspheres or beads, including oil-in-water emulsions, micelles, mixed micelles, synthetic membrane vesicles and released red blood cells. Any pharmaceutically acceptable formulations such as polymers or natural polymers are included. In addition to the compounds and pharmaceutically acceptable polymers, the pharmaceutically acceptable formulations used in the methods of the present invention may further comprise pharmaceutically acceptable carriers and / or excipients. In the present specification, pharmaceutically acceptable carriers include all solutions, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents and anything else physiologically equivalent. For example, the carrier may be suitable for infusion into the blood. Excipients include pharmaceutically acceptable stabilizers and disintegrants. In another embodiment, the pharmaceutically acceptable formulation comprises a lipid based formulation. Any known lipid based drug delivery system can be used in the practice of the present invention. For example, so-called multi-vescular liposomes (MVL), multi-lamellar liposomes (also known as multilamellar vesicles or MLVs), small single single, as long as they can exhibit consistent release rates of encapsulated compounds. Both single-lamellar liposomes, including lamellar liposomes (also known as small single-lamellar vesicles or small uni-lamellar vesicles) and large single-lamellar liposomes (also known as large single-lamellar vesicles or large uni-lamellar vesicles) can be used. have. As one of the embodiments, the lipid based formulation may be a somewhat liposome system. Compositions of synthetic membrane vesicles are generally combinations of phospholipids, steroids, especially cholesterol. Other phospholipids or lipids may also be used. Examples of lipids useful as synthetic membrane endoplasmic reticulum products include phosphatidylglycerol, phosphatidylcholine, phosphatidylserine, phosphatidylethanolamino, sphingolipids, cerebrosides and gangliosides. Good phospholipids to use include egg phosphatidylcholine, dipalmitoylphosphatidylcholine, distearoylphosphatidylcholine, dioleoylphosphatidylcholine, dipalmitoylphosphatidylglycerol and dioleoylphosphatidylglycerol. In yet another embodiment, the composition comprising the compound may be bound or saturated into a bioabsorbable matrix. In addition, the substrate may be made of the biopolymer.

Suitable biopolymers of the invention also include collagen, elastin, fibronectin, vitronectin, laminin, polyglycolic acid, hyaluronic acid, chondroitin sulfate, dermatan sulphate, heparin, heparin, fibrin, cellulose, gelatin, Consists of polylysine, echinonectin, entactin, thrombospondin, uvomorulin, biglycan, decorin and dextran At least one macromolecule selected from the group is included. Formulations of such macromolecules into biopolymers are well known in the art. In an embodiment, the therapeutic composition preferably does not exhibit immunogenicity upon administration to a human patient for the purpose of treatment.

Therapeutic compositions of the invention may include pharmaceutically acceptable salts of the constituents thereof. The pharmaceutically acceptable salts include, for example, acid addition salts formed using inorganic acids such as hydrochloric acid or phosphoric acid or organic acids such as acetic acid, tartaric acid, mandelic acid and the like. Physiologically tolerable carriers are well known in the art. Examples of liquid carriers include sterile aqueous solutions containing no active ingredients and substances other than water, or sterile aqueous solutions containing both buffers such as sodium phosphate, physiological saline or phosphate buffered saline at physiological pH values. This includes. Aqueous carriers may also include salts of two or more buffering salts as well as sodium chloride and potassium chloride, glucose, propylene glycol, polyethylene glycol and other solutes. The liquid composition may further comprise a liquid phase in addition to water. Examples of such additional liquid phases are glycerin, vegetable oils such as cottonseed oil, organic compounds such as ethyl oleate, and water-oil emulsions. Therapeutic composition

The term “pharmaceutically acceptable salts” refers to compounds obtained by reaction with inorganic acids such as hydrochloric acid, bromic acid, sulfuric acid, nitric acid, phosphoric acid, methanesulfonic acid, ethanesulfonic acid, toluenesulfonic acid, salicylic acid, and the like of the biological of the free base. It refers to salts of compounds that possess efficiency and properties. Pharmaceutical compositions comprising the active ingredient may be suitable formulations for oral use, eg, tablets, troches, lozenges, aqueous or oily suspensions, dispersible powders or granules, emulsions, hard or soft capsules. Or syrup or elixirs. Oral compositions can be prepared according to any method known in the art for the preparation of pharmaceutical compositions, which compositions are prepared from the group consisting of sweeteners, flavoring agents, coloring agents and preservatives for the preparation of pharmaceutically elegant and delicious tastes. At least one agent selected. Tablets containing the active ingredient in admixture with non-toxic and pharmaceutically acceptable excipients are suitable for the manufacture.

Such excipients include, for example, inert diluents such as calcium carbonate, sodium carbonate, lactose, calcium phosphate or sodium phosphate, granulating and disintegrating agents such as corn starch or alginic acid, binders such as starch, gelatin or acacia, magnesium stearate, Lubricants such as stearic acid or talc. Tablets may be uncoated and may be coated using known techniques to delay disintegration and absorption in the gastrointestinal tract to provide consistent activity over longer periods of time. For example, a time delay substance such as glyceryl monostearate or glyceryl distearate can be used. The tablets may also be coated using techniques to form osmotic therapeutic tablets for controlled release disclosed in US Pat. Nos. 4,256,108, 4,166,452 and 4,265,874. The pharmaceutical composition may also or alternatively comprise one or more medicaments, which may be bound to a modulator or may be free in the composition. Indeed, any medicament may be administered in combination with a modulator described herein for the various purposes described below. Examples of the types of medications that may be administered with modulators include analgesics, anesthetics, antianginal, antifungal, antibiotic, anticancer agents (eg cisplatin, taxol or mitomycin C), anti-inflammatory agents (eg ibuprofen and indomethacin), Insect repellents, antidepressants, antidotes, antiemetics, antihistamines, antihypertensives, antimalarials, antimicrotubule agents (eg colchicine or vinca alkaloids), migraine medications, antibacterials, antipsychotics, antipyretics, antiseptics, anti-signaling Agents (e.g., protein kinase C inhibitors or intracellular calcium migration inhibitors), anti-arthritis agents, antithrombin drugs, tuberculosis drugs, cough medicines, antiviral drugs, appetite suppressants, cardiac agents, chemical dependency drugs, diarrhea, chemical Therapy, cardiovascular, cerebrovascular or peripheral vasodilators, contraceptives, inhibitors, diuretics, expectorants, growth factors, hormones , Sleeping pills, immunosuppressants, drug antagonists, parasympathetic stimulants, sedatives, stimulants, sympathetic stimulants, toxins (eg, cholera toxins), sedatives and therapeutic agents for urinary infections.

Oral formulations may be present in hard gelatin capsules containing the active ingredient mixed with an inert solid diluent such as calcium carbonate, calcium phosphate or kaolin, the active ingredient being an oily medium such as water or peanut oil, liquid papain or olive oil. It may also be present as a soft gelatin capsule in admixture with.

Aqueous suspensions contain the active ingredient in admixture with excipients suitable for the manufacture thereof. Such excipients are suspending agents, for example sodium carboxymethylcellulose, methylcellulose, hydroxy-propylmethylcellulose, sodium alginate polyvinylpyrrolidone, gum tragacanth and acacia rubber; Dispersants or wetting agents are naturally occurring phospholipids such as lecithin, or condensation products of alkylene oxides and fatty acids, such as polyoxyethylene stearate, or condensation products of long chain aliphatic alcohols and ethylene oxide, such as heptadecaethyleneoxycetanol Or condensation products of ethylene oxide with partial compounds derived from fatty acids and hexitol such as polyoxyethylene and the like, and partial compounds derived from fatty acids and anhydrous hexitol such as polyoxyethylene sorbitan monooleate (anhydrous sorbitol monooleic acid) to be. The aqueous suspension also includes ethyl or one or more preservatives such as n-propyl, p-hydroxybenzoate, one or more coloring agents, one or more flavoring agents and one or more sweetening agents such as sucrose or saccharin. .

Oily suspensions can be formed by suspending the active ingredient in vegetable oils such as arachis oil, olive oil, sesame oil or coconut oil, or by suspending in inorganic oils such as liquid paraffin or the like. Oily suspensions may include, for example, thickening agents such as beeswax, hard paraffin or cetyl alcohol. Sweetening agents and flavoring agents such as those described above may be added to provide oral preparations suitable for taste. Such compositions can be preserved by the addition of antioxidants such as ascorbic acid.

Dispersible powders and granules suitable for the preparation of an aqueous suspension by addition of water provide the active ingredient in the form of a dispersing or wetting agent, suspending agent and one or more preservatives. Illustrative suitable dispersing or wetting agents and suspending agents, such as sweetening, flavoring and coloring agents, may also be present.

The pharmaceutical compositions of the present invention may also be in the form of oil-in-water emulsions. The oil phase may be a vegetable oil such as olive oil or arachis oil, or an inorganic oil such as liquid paraffin, or a mixture thereof. Suitable emulsions include naturally occurring rubbers such as acacia rubber or tragacanth rubber, naturally occurring phospholipids such as soybean and lecithin, compounds or partial compounds derived from fatty acids such as sorbitan monooleate and hexitol anhydrous, and polyoxyethylene sorbitan Condensation products of such partial compounds and ethylene oxide, such as monooleate. Emulsions may also include sweetening and flavoring agents.

Syrups and elixirs can be prepared with sweetening agents such as glycerol, propylene glycol, sorbitol or sucrose. Such formulations may also include emollients, preservatives and flavoring and coloring agents. The pharmaceutical composition may be in the form of a sterile injectable aqueous or oleagenous suspension. Such suspensions can be prepared according to methods known in the art using suitable dispersing or wetting agents and suspending agents as described above.

Sterile injectable formulations may be sterile injectable solutions or suspensions of nontoxic and externally acceptable diluents or solvents, such as 1,3-butane diol solutions. Among the acceptable carriers and solvents that may be employed are water, Ringer's solution and isotonic sodium chloride solution. In addition, sterile, nonvolatile fats and oils are commonly used as a solvent or suspending medium. For this purpose, any nonvolatile mixed fat or oil may be used including synthetic monoglycerides or diglycerides. Moreover, fatty acids such as oleic acid can be used in the preparation of injectables.

The amount of active ingredient to combine with a carrier material to obtain a single dosage form will vary depending upon the particular dosage form and subject to be treated. For example, formulations for oral administration to humans can vary from about 5% to about 95% of the total composition. Unit dosage forms generally comprise between about 1 mg and about 500 mg of the active ingredient. However, the specific dosage level for any patient is determined by the activity, age, weight, usual state of health, sex, diet, duration of administration, route of administration, rate of excretion, drug combination, and therapy of the specific compound used. It will depend on a variety of factors, including the severity of it. The dosage effective amount of a compound according to the invention will vary depending on factors including the specific compound, toxic and inhibitory activity, treatment conditions and whether the compound is administered alone or in combination with other therapies. Generally, the dosage effective amount is from about 0.0001 mg / kg to about 1500 mg / kg, preferably from 1 to 1000 mg / kg, more preferably from about 1 to 150 mg / kg, the best being about 50 to 100 mg / kg.

The compounds of the present invention can be administered to warm blooded animals, such as humans, which require such treatment in an effective amount corresponding to the diseases described above for prophylaxis or treatment, which compounds are preferably used in the form of pharmaceutical compositions.

Formulations of pharmaceutically acceptable excipients and carrier solutions are well known to those of ordinary skill in the art and for various therapeutic regimens and various formulations, including oral, extragranular, intravenous, intranasal, and intramuscular. The development of appropriate dosage and therapeutic regimens using the particular compositions described herein is also well known to those skilled in the art.

1.1. Oral delivery

In any application, the pharmaceutical compositions described herein can be delivered to the animal in oral dosage form. In such cases, the composition may be prepared with an inert diluent or an assimitable edible carrier, or it may be enclosed in a hard or soft shell gelatin capsule, or compressed into tablets, or during meals. It can also be mixed directly with the food.

The active compounds may also be used in the form of tablets, oral tablets, troches, capsules, elixirs, suspensions, syrups, wafers, etc., which are ingested in admixture with excipients. Tablets, troches, pills, capsules and the like may also include the following: binders such as tragacanth gum, acacia, corn starch or gelatin and the like; Excipients such as calcium diphosphate; Disintegrants such as corn starch, potato starch, alginic acid and the like; Glidants such as magnesium stearate and sweeteners such as sucrose, lactose or saccharin may be included, or may include flavorings such as peppermint, wintergreen oil or cherry flavor. If the unit dosage form is a capsule, liquid carriers may be included in addition to the above types of materials. Various other materials may be present as coatings or may otherwise alter the physical form of the unit dosage form. For example, tablets, pills or capsules may be coated with shellac, sugar or both. Elixir syrups contain the active compound sucrose as a sweetener, methylparaben and propylparaben as preservatives, dyes and Sweeteners such as cherry or orange flavors. Of course, any material used to prepare any unit dosage form must be pharmaceutically pure and substantially non-toxic in the amount used. In addition, the active compounds can be used in combination in delayed release formulations and formulations.

In general, such formulations may comprise at least about 0.1% of the active ingredient by weight or volume of the total formulation, although typically from about 1 or 2% to about 60%, although the percentage of active ingredient may naturally vary. Or 70% or more. In general, the amount of active compound in each therapeutically useful composition that can be prepared is determined in the manner of a suitable dosage that can be obtained in any given unit dosage of this compound. Those skilled in the art may consider factors such as solubility, biocompatibility, biological half-life, route of administration, shelf life of the product, as well as other pharmacological considerations in the manufacture of such pharmaceutical formulations, and various dosages and treatments. It is good to consider therapy.

For oral administration, the compositions of the present invention may be mixed with one or more excipients in the form of mouthwashes, toothpastes, oral tablets, oral sprays or sublingual oral dosage forms. For example, mouthwashes can be prepared by mixing with the required amount of active ingredient in a suitable solvent, such as sodium borate solution. In addition, the active ingredient may be mixed in an oral solution such as sodium borate, glycerin and potassium bicarbonate, or dispersed in a toothpaste, or in a composition which may contain water, binders, abrasives, flavors, foaming agents and humectants. It can be added in a pharmaceutically effective amount. The compositions may also be prepared in the form of tablets or solutions that can be placed under the tongue or otherwise dissolved in the mouth.

1.2. Injection delivery

In some cases, it may be desirable to use an intestinal, intravenous, intramuscular or even intraperitoneal delivery method for the pharmaceutical composition of the present invention. Active compound solutions as free base or pharmaceutically acceptable salts can be prepared in aqueous solution suitably mixed with a surface active agent such as hydroxypropylcellulose. Dispersions can also be prepared as glycerol solutions, liquid polyethylene glycol solutions and mixed solutions thereof, and oil and fat solutions. Under ordinary conditions of storage and use, these forms of preparation include a preservative to prevent the growth of microorganisms.

Pharmaceutical formulations suitable for injection include sterile aqueous solutions or sterile dispersions and sterile powders for the temporary preparation of sterile injectable solutions or dispersions. In all cases, the formulation must be sterile and must be fluid to the extent that it can be easily released from the syringe. It must also be stable under the conditions of manufacture and storage and must be able to be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be, for example, a solvent or dispersion medium comprising water, ethanol, polyols (eg, glycerol, propylene glycol and liquid polyethylene glycols, etc.), appropriate mixtures thereof, and / or vegetable oils and the like. Suitable fluidity can be maintained, for example, by using a coating such as lecithin, in the case of dispersions by maintaining the required particle size, and by using surface active agents. Various antibacterial and antifungal agents can be used to protect against the action of microorganisms such as parabens, chlorobutanol, phenol, sorbic acid, thimerosal and the like. In many cases, it may be desirable to include isotonic agents, such as sugar or sodium chloride. Absorption of absorption agents such as aluminum monostearate and gelatin in the composition can be used to prolong the absorption of the injectable composition.

In aqueous solutions for intestinal administration, for example, the solution should be properly buffered if necessary and the liquid diluent first made into an isotonic solution with sufficient saline or glucose. Such special aqueous solutions are particularly suitable for intravenous, intramuscular, subcutaneous and intraperitoneal administration. In this regard, those skilled in the art will recognize sterile aqueous media that may be used with reference to the present specification. For example, one dose can be dissolved in 1 ml of isotonic NaCl solution and 1000 ml of bulk hypodermoclysis fluid can be added or injected into the desired injection site. Some dosage change will be necessary depending on the condition of the patient being treated. In any case, the medication operator will have to determine the appropriate dosage individually for the patient. In addition, medications for humans must meet the general standard of safety and purity required by the Office of National Standards for Sterilization, Pyrogenicity and National or Regional Biological Standards.

Sterile injectable solutions are prepared by mixing the required amount of active compound in a suitable solvent with some of the other ingredients described above, followed by filtration and sterilization. Generally, dispersions are prepared by incorporating the various sterile active ingredients into a sterile vehicle comprising a basic dispersion medium and mixing the necessary other ingredients as described above. In the case of sterile powders, preferred methods of preparation for sterile injectable solutions are vacuum drying and freeze drying to obtain powders of the active ingredient and other desired additional ingredients from their presterile filtered solution.

The compositions described herein can be prepared in the form of neutrals or salts. Pharmaceutically acceptable salts include acid addition salts (formed using protein free amino groups) and are formed using inorganic acids such as hydrochloric acid or phosphoric acid, or organic acids such as acetic acid, oxalic acid, tartaric acid, mandelic acid, and the like. Salts formed using free carboxyl groups can also be derived from inorganic bases such as hydroxides of sodium, potassium, ammonium, calcium or iron and the like and organic bases such as isopropylamine, trimethylamine, histidine, procaine and the like. In view of the formulation, the solution will be administered in a manner suitable for the dosage formulation and will be administered in an amount suitable for a therapeutically effective amount. The formulations are easily administered in a variety of dosage forms, including injection solutions, drug release capsules, and the like.

As used herein, "carrier" includes any and all solvents, dispersion media, carriers, coatings, diluents, antibacterial and antifungal agents, isotonic and absorption delaying agents, buffer solutions, carrier solutions, suspensions, colloids, and the like. The use of such media and formulations as pharmaceutically active substances is well known in the art. Except insofar as any conventional media or agent is not suitable for the active ingredient, use may be contemplated for use in the therapeutic compositions. Supplementary active ingredients can also be mixed into the compositions.

1.3. Nasal delivery

In some embodiments, intranasal spray, inhalation, and / or other aerosol delivery vehicles may be used to deliver the pharmaceutical composition. Similarly, pharmaceutical delivery using intranasal microparticle resins and lysophosphatidyl-glycerol compounds is also well known in the pharmaceutical art.

2. Target Cancer

Patients to be treated will generally include mammals, most preferably human patients, such as human cancer patients. The compounds of the present invention can be used alone or in combination. In addition, therapeutic compounds may be used in conjunction with other types of treatment. For example, the compounds of the present invention may be combined with other chemotherapy such as cisplatin, tamoxifen, taxol, methotrexate, biomaterials such as antibodies, growth factors or lymphokines, radiation, etc. Can be used. Combinations of therapies can produce synergistic effects. The indication is preferably cancer, in particular the cancers described above.

The compositions and methods provided herein appear particularly useful for primary and metastatic tumors, including solid tumors such as breast, central nervous system, colon, ovary, kidney, lung, liver, bladder, prostate, head and neck. More specifically, tumors that can be treated by the compositions and methods of the present invention include the following epithelial tumors:

It is not limited to this.

Lungs: Bronchogenic carcinoma (squamous cell carcinoma, undifferentiated small cell carcinoma, undifferentiated large cell carcinoma, adenocarcinoma), alveolar (bronchiolary) carcinoma, bronchial adenocarcinoma, sarcoma, lymphoma, Chondroma, mesothelioma, mesothelioma;

Gastrointestinal: esophagus (squamous cell carcinoma, adenocarcinoma), gastric carcinoma, colorectal carcinoma;

Genitourinary tract: kidney (adenocarcinoma, Wilm tumor) [renal blastoma], lymphoma, leukemia), bladder and urethra (squamous cell carcinoma, transitional cell carcinoma, adenocarcinoma), prostate (adenocarcinoma, sarcoma), testicles (stemoma) );

Liver: liver cancer (hepatocellular carcinoma);

Bone: osteogenic sarcoma (osteosarcoma);

Nervous system: neuroblastoma, retinoblastoma, glioblastoma, oligodendroglioma;

Gynecology: cervix (cervical carcinoma, pre-tumour cervical dysplasia);

Hematology: blood (myeloid leukemia [acute and chronic], acute lymphoblastic leukemia, chronic lymphocytic leukemia), Hodgkin's disease, non-Hodgkin's lymphoma [malignant lymphoma];

Skin: malignant melanoma, basal cell carcinoma, squamous cell carcinoma, Karposi sarcoma; And

Adenocarcinoma: Adenocarcinoma, Papillary carcinoma and Papillary adenocarcinoma.

Thus, as used herein, "cancer cell" is a term that includes a cell suffering from any one of the disease conditions specified above.

Hereinafter, the present invention will be described.

In the present invention, the expression level of Nox was analyzed in early and late stage human breast cancer cell lines and their role in carcinogenic EMT and infiltration process was investigated. In addition, we determined whether the acquisition of these cancer cell lines, which are resistant to the anti-cancer drug cisplatin, is due to the activity of specific Nox protein. Furthermore, the inhibitory effect of EMT induction and invasion of cancer cells and the synergistic effect on cancer cell death in the composition of cisplatin were identified using diphenyleioniodonium, an inhibitor of Nox.

Hereinafter, the present invention will be described in detail.

In normal mammary and metastatic breast cancer cells Reactive oxygen species  And Nox  Comparison of gene expression levels

   The intracellular levels of reactive oxygen species were analyzed for differences in normal cells and cancer cells. As shown in FIG. 1A, the levels of reactive oxygen species were significantly higher in the two representative metastatic breast cancer cells MDAMB-231 (7.14 fold) and Hs578T (13.86 fold) compared to normal mammary cell MCF10A. In addition, it was confirmed that mRNA expression of Nox1, Nox4 and Nox5 was also significantly increased in both metastatic cancer cells (FIG. 1B).

Of metastatic breast cancer cells Reactive oxygen species As a generator Nox  Determining the Role of Proteins

The active oxygen species generation of metastatic breast cancer cells was investigated. The major sources of reactive oxygen species in cells are the production of Mitochonria from electron transfer system, metal ion reaction by Xanthine oxidase and NADPH oxidase.

In the present invention, the analysis was focused on the role of Nox1, 4 and Nox5 in the Nox family. In order to elucidate the role of each Nox protein, small hairpin RNA (shRNA) plasmid DNA that degrades mRNA generated from intracellular Nox genes was prepared to prepare stable transgenic cell lines which were permanently introduced into intracellular chromosomes. In order to measure the activity of NADPH oxidase, an assay for measuring the production of superoxide was applied. As shown in FIG. 2A, the production of superoxide was significantly reduced in the cell lines into which shRNA Nox1, shRNA Nox4, and shRNA Nox5 were introduced, respectively, compared to the control cell line into which only the vector plasmid was introduced. This reduction was greatest in triple transgenic cell lines in which all three shRNA Nox were introduced. Consistent with these results, when treated with diphenylene iodonium (DPI), an inhibitor of NADPH oxidase activity, the production level of superoxide decreased in proportion to the concentration change (FIG. 2B).

In metastatic cancer cell lines Nox  E- by protein cadherin  And Snail1 Expression change of

EMT, one of the characteristics of cancer cells in the early stages of malignancy, is essential for tumor invasion and metastasis. Therefore, the discovery of target regulators that can artificially regulate EMT is very important to block tumor progression. In general, the expression of E-cadherin, an epithelial marker protein that plays a key role in the EMT process in metastatic cancer cells, is reduced, and the expression of Snail1, a marker protein of mesenchymal derived cells, is increased.

As shown in Figure 3A, it can be seen that the morphology of metastatic cancer cells treated with DPI, a Nox inhibitor, is remarkably recovered from fibroblasts to epithelial cells. This reverse MET process of EMT was demonstrated at the protein level through the result that E-cadherin (green fluorescence) expression was restored and Snail1 (red fluorescence) expression was reduced under the same DPI treatment conditions (FIG. 3B). Furthermore, it could be seen that the opposite expression changes of the two proteins occur equally at the mRNA level (FIG. 3C).

To demonstrate the effect of DPI once again, shRNA systems that inhibit the expression of Nox1, Nox4, Nox5 or Nox1 / 5/5 were analyzed in metastatic cancer cell lines introduced into intracellular chromosomes. The same results as in FIG. 3C were obtained at the mRNA level (FIG. 3D). The effect of this expression change was significantly higher in cell lines where Nox1, Nox4 and Nox5 were all inhibited.

Migration of metastatic cancer cells and Infiltration degree  In DPI  And shNox1 , shNox4 , shNox5 Inhibitory Effect of

The effects of DPI and shRNA Nox1, Nox4, Nox5 or Nox1 / 4/5 on the migration and invasion ability of cancer cells induced by EMT were investigated.

In the case of metastatic cancer cell lines treated with various concentrations of DPI, it was confirmed that the migration of cells is significantly reduced (FIG. 4A). In addition, it was confirmed that the degree of inhibition of DPI appeared in the level of 70% compared to the control even in the experiment measuring the degree of cancer cell infiltration through the Traswell migration assay (FIG. 4B). The inhibitory effect of DPI on cancer cell invasion was shRNA Nox1 (lower than 55%), shRNA Nox4 (lower than 52%), shRNA Nox5 (lower than 45%) or shRNA Nox1 / 4/5 (lower: 78%) were demonstrated at comparable levels even in metastatic cancer cells (FIG. 4C).

DPI Wow cisplatin  Synergistic effect of metastatic cancer cell death by complex composition

The main cause of death in terminal cancer patients is the resistance of cancer cells to chemotherapy agents such as anticancer agents. In the case of cancer cells, it is estimated that the activity of the antioxidant system is increased by increasing the level of reactive oxygen species in the cell, thereby increasing its own viability. This possibility is expected to be high through the increase of intracellular reactive oxygen species by increasing Nox protein expression in metastatic cancer cells and the resultant increase in EMT and infiltration. As shown in FIG. 5A, when treated with DPI or cisplatin alone, death of cancer cells did not increase. On the other hand, treatment with 5 μM DPI with 100 μM cisplatin significantly increased apoptosis of cancer cells. This effect was also reaffirmed in increasing cleavage of PARP, which is used as a marker protein for apoptosis (FIG. 5B). Furthermore, staining of annexin V (G2 phase; late apoptosis, G4 phase: early apoptosis), which indicates the degree of cell death by binding to the membrane lipid component of the cell to be killed, was also increased significantly when the two substances were combined (FIG. 5C).

NOX1 , NOX4 Wow NOX5 end knock - down Done MDA - MB231  Cell cisplatin Reduced tolerance

MDA-MB231 cells are resistant to apoptosis induced by Cisplatin. MDA-MB231-shRNA NOX1 / 4/5 cells, which inhibited the expression of NOX1, NOX4, and NOX5, significantly increased cell death susceptibility by cisplatin as compared to MDA-MB231-pLKO.1 cells (FIG. 6A and 6B).

As can be seen from the above problem solving means and the accompanying drawings, the present invention first identified the role of the Nox protein as a trigger for acquiring cancer tumor malignancy and resistance to anticancer drugs. In addition, the role of Nox1, Nox4, and Nox5 in cancer metastasis and survival of cancer cells was first identified. In particular, it can be seen through the present invention that diphenylene iodide (DPI), a Nox inhibitor, has the effect of killing cancer cells through a combination composition with an anticancer agent, cisplatin, and if a more effective DPI derivative is discovered, the development of a cancer treatment agent is also greatly improved. There is an effect that can contribute.

1 is an experimental result of analyzing the production level of reactive oxygen species (A) and NOX1, NOX4, NOX5 and GAPDH mRNA expression level (B) in normal mammary cell MCF10A and metastatic breast cancer cells MDA-MB231 and Hs578T.
Figure 2 shows the time-dependent amount of superoxide produced by MDA-MB231, a metastatic cancer cell expressing control vector (pLKO.1), shRNA NOX1, shRNA NOX4, shRNA NOX5 or shRNA NOX1 / NOX4 / NOX5, and (A) DPI, the NOX inhibitor DPI. Experimental results show the amount of superoxide produced in starch-treated MDA-MB231 cell line.
Figure 3 shows the results of (A) metastatic cancer cells MDA-MB231 and Hs578T is converted to epithelial cells from mesenchymal-derived cells when treated with a NOX inhibitor DPI (2.5 mM) (12 hours). Morphology of the cells was taken using a phase contrast microscope (magnification, X 200). (B) Hs578T cells were treated with NOX inhibitor DPI (2.5 mM) (12 hours) and the expression changes of E-cadherin and Snail1 were directly analyzed on cells by immunostaining using confocal laser microscopy. (C) The expression changes of E-cadherin, Snail1, and GAPDH in MDA-MB231 and Hs578T cells were treated with concentrations of NOX inhibitor DPI (12 hours) at the mRNA level by RT-PCR method. (D) of E-cadherin, Snail1, NOX1, NOX4, NOX5 and GAPDH in MDA-MB231, a metastatic cancer cell expressing the control vector (pLKO.1), shRNA NOX1, shRNA NOX4, shRNA NOX5 or shRNA NOX1 / NOX4 / NOX5 Expression changes were analyzed by RT-PCR method at the mRNA level.
Figure 4 (A) MDA-MB231 and Hs578T cells were treated with a concentration of NOX inhibitor DPI to the (12 hours) to compare the cell migration capacity. Cells were distributed 100% in a 6-well culture dish and then scratched to a certain extent. After washing with PBS, the culture medium was exchanged and the cells were incubated for 12 hours to investigate their migration capacity by DPI treatment. (B) The infiltration rate of the cells passing through the membrane filter of Hs578T cells was analyzed under the same conditions. (C) The invasion of MDA-MB231 cells, metastatic cancer cells expressing control vector (pLKO.1), shRNA NOX1, shRNA NOX4, shRNA NOX5 or shRNA NOX1 / NOX4 / NOX5, was analyzed. Each bar graph represents the number of infiltrating cells.
Figure 5 (A) MDA-MB231 and Hs578T cells were examined for the degree of apoptosis when treated with NOX inhibitors DPI (2.5 mM), cisplatin (100 mM), or DPI plus cisplatin. Necrotic detached and circular cells were photographed with a phase contrast microscope (x 200 magnification). (B) MDA-MB231 and Hs578T cells were treated with NOX inhibitors DPI (2.5 mM), cisplatin (100 mM), or DPI plus cisplatin (24 hours) to obtain a cell extract to express the degree of cell death. The degree of cleavage of poly (ADP-ribose) polymerase (PARP) was analyzed by Western blotting. b-actin shows that the total amount of protein used is the same. (C) The composition of prodium iodide (PI, longitudinal axis) and Annexin V (horizontal axis) indicating the degree of apoptosis in the condition of treating the MDA-MB231 cells by forming a complex composition with cisplatin (100 mM) according to the concentration of the NOX inhibitor DPI The staining degree was investigated by flowcytomety analysis.
Figure 6 is a diagram showing the synergistic effect of apoptosis by cisplatin in the MDA-MB 231 cell line into which the sh RNA Nox 1/4/5 gene was introduced. (A) shows morphological changes of cells (B) shows FACS analysis. The degree of cell death by

Hereinafter, the present invention will be described in more detail with reference to non-limiting examples. However, the following examples are described for the purpose of illustrating the present invention and the scope of the present invention is not to be construed as being limited by the following examples.

Diphenyl iodonium (DPI) and cisplatin used in the present invention were purchased from Sigma. H2DCFDA fluorescent dye was purchased from Molecular Probe Company. PARP and beta-actin antibodies were purchased from Santa Cruz Biotechnology.

Example  1: Cell line culture

Human breast cancer cell lines MCF-7, MDA-MB231 and Hs578T were purchased from American Cell Line Strain Bank (American Type Culture Collection (ATCC)). Each cell line was incubated in a cell incubator at 37 ° C. with 5% CO 2 gas using Dulbecco's modified Eagle's medium (DMEM) medium containing 10% Fetal bovine serum (FBS).

Example  2: shRNA Nox  Gene recombinant plasmid preparation

The oligonucleotides of SEQ ID NOs: 1 to 6 were synthesized, denaturated at 90 ° C., and then slowly annealed at room temperature. Annealed oligonucleotides were cut with Age I and EcoRI, and then conjugated to Age I / EcoR I cloning sites of pLKO.1-TRC. Electrophoresis confirmed the completion of the recombinant plasmid.

human Nox1 sh oligo Fw (SEQ ID NO: 1)

CCGGATTCTCTCCAGCCTATCTCATCTCGAGATGAGATAGGCTGGAGAGAATTTTTTG

human Nox1 sh oligo Re (SEQ ID NO: 2)

AATTCAAAAAATTCTCTCCAGCCTATCTCATCTCGAGATGAGATAGGCTGGAGAGAAT

human Nox4 sh oligo Fw (SEQ ID NO: 3)

CCGGAGCAAGATACCGAGATGAGGACTCGAGTCCTCATCTCGGTATCTTGCTTTTTTG

human Nox4 sh oligo Re (SEQ ID NO: 4)

AATTCAAAAAAGCAAGATACCGAGATGAGGACTCGAGTCCTCATCTCGGTATCTTGCT

human Nox5 sh oligo Fw (SEQ ID NO: 5)

CCGGTGTTCTATTGGACTCACCTGTCTCGAGACAGGTGAGTCCAATAGAACATTTTTG

human Nox5 sh oligo Re (SEQ ID NO: 6)

AATTCAAAAATGTTCTATTGGACTCACCTGTCTCGAGACAGGTGAGTCCAATAGAACA

Example  3: Genetically Modified Cell Line Construction

pLKO1, pLKO1-shNox1, pLKO1-shNox4, and pLKO1-shNox5 plasmid DNA (1 μg) were introduced into 293T cells, which are viral host cells, along with psPAX2 packaging plasmid (0.75 μg) and pMD2 envelop plasmid (0.25 μg), respectively. After 12 hours, the medium was exchanged and further incubated for 24 hours, and the cell culture solution containing the virus was collected and centrifuged (1,000 rpm, for 5 min). The supernatant virus isolated by filtering was infected with MDA-MB231, a target cell. At this time, Polybrene (8 μg / ml) was treated together. After removing the culture dish every 6 hours, the virus was precipitated by centrifugation at 500 rpm for 30 minutes, and then the culture process was repeated 5 times. After replacement with fresh medium, the antibiotic puromycin was treated with 2 μg / ml. Every 2-3 days, the cells were harvested for 3 weeks while being replaced with a medium containing antibiotics. Expression of Nox1, Nox4 and Nox5 was finally confirmed by RT-PCR.

Experimental Example  One: Intracellular Reactive oxygen species  Concentration measurement

100,000 MDA-MB231 cells were seeded on a 6-well plate. After 24 hours, propodidium iodine was treated at the indicated concentrations, followed by an additional 12 hours of incubation and 5 μM DCFDA treatment 30 minutes before cell collection. After washing with PBS, cells were detached by treatment with Trypsin-EDTA and centrifuged for 5 minutes at 600 rpm. After the precipitate was dissolved in PBS, flow cytometry was used to quantitatively measure the amount of reactive oxygen species distributed per 10,000 cells.

Experimental Example  2: NADPH oxidase  Active measurement

NADPH oxidase activity was identified by measuring superoxide production levels. 100,000 MDA-MB231 cells were seeded in a 6-well plate. After 24 hours, propiodium iodide was treated at the indicated concentrations and further incubated for 12 hours. After washing with PBS, cells were detached by treatment with Trypsin-EDTA and centrifuged for 5 minutes at 600 rpm. The precipitate was dissolved in 450 μl PBS, and then reacted with 50ul of 2.5 mM lucigenin (Sigma) and 1ul of 100 mM NADPH (Calbiochem). Superoxide production was measured at 10 second intervals using a luminometer (BD Monolight ™ 3010C Luminometer).

Experimental Example  3: RNA  Tablets and Reverse transcription  Enzyme-gene amplification reaction ( reverse transcriptase -polymerase chain reaction )

RNA is phenol-guanidinium isothiocyanate method [Chomczynski, P. and Sacchi, N. (1987) Anal. Biochem. 162, 156-159]. Reverse transcriptase-gene amplification reactions were performed by the instructions of the Acess RT-PCR system (Promega, Madison, Wis.). The base sequence of the primer used is as follows.

Human Nox1-specific primers forward (SEQ ID NO: 7) 5 GTA CAA ATT CCA GTG TGC AGA CCA C 3 'reverse (SEQ ID NO: 8) 5'GAG ACT GGA ATA TCG GTG ACA GCA3'

human Nox4-specific primers forward (SEQ ID NO: 9) 5 GTT CTT AAC CTC AAC TGC AGC C 3 'reverse (SEQ ID NO: 10) 5'GCA TAT GTA GAG GCT GTG ATC 3'

human Nox5-specific primers forward (SEQ ID NO: 11) 5 ATG AGT GGC ACC CCT TCA CCA TCA G 3 'reverse (SEQ ID NO: 12) 5 GTC AGC AGG CTC ACA AAC CAC TCG AA 3'

human E-cadherin-specific primers forward (SEQ ID NO: 13) 5 CCT TCC TCC CAA TAC ATC TCC C 3 'reverse (SEQ ID NO: 14) 5'TCT CCG CCT CCT TCT TCA TC 3'

human Snail1-specific primers forward (SEQ ID NO: 15) 5 GGG CAG GTA TGG AGA GGA AGA 3 'reverse (SEQ ID NO: 16) 5'TTC TTC TGC GCT ACT GCT GCG 3'

human beta actin-specific primers forward (SEQ ID NO: 17) 5 ACG TTG CTA TCC AGG CTG TG 3 'reverse (SEQ ID NO: 18) 5'GCG ACG TAG CAC AGC TTC TC 3'

Reverse transcriptase-gene amplification reactions were performed in steps 1 (5 min at 95 ° C.), 2 steps (35 cycles of 94 ° C. 30 sec, 55 ° C. 30 sec, and 72 ° C. 1 min) and 3 steps (72 ° C. 10 min, 4 Under cooling). The amplified product was isolated by 0.8% agarose electrophoresis and fluorescence was detected under UV lamp by ethidium bromide staining.

Experimental Example  4: Intracellular Protein Fluorescence

50,000 Hs578T and MAD-MB231 cell lines were seeded on an 18 mm glass coverslip. After 24 hours, the material was treated and fixed in 3.5% ice cold paraformaldehyde for 5 minutes. After 2 minutes of ice cold methanol permeabilization, and soaked in 5 mM glycine solution for 5 minutes. Intracellular E-cadherin and Snail1 proteins were labeled with anti-E-cadherin rabbit polyclonal antibody and anti-Snail1 mouse polyclonal antibody. After washing the cells twice with PBS (5 minutes each), the fluorescence isothiocyanate (FITC) conjugated goat anti-mouse IgG or rhodamine (TRITC) conjugated goat anti-rabbit IgG secondary antibody was reacted in a dark room for 1 hour. Stained cells were washed three times with PBS, mounted with a solution containing 4,6-diamino-2-phenylindole (DAPI), and analyzed using an Olympus Fluoview-FV300 confocal microscope.

Experimental Example  5: membrane transfer penetration ( Transwell migration ) assay

After dissolving Matrigel (BD Cat # 356234) at 4 ° C, it was only diluted 1: 9 in DMEM medium, and put into 30 μl in the upper chamber and dried at room temperature for 2 hours. The transfected cells were detached after trypsin treatment and 10 ⁴ MDA-MB231 cells were put together with 200 ml of serum free-medium in an upper chamber (BD Cat # 353097) coated with matrigel. The lower chamber was treated with 100 ng / ml EGF in 500 µl growth medium and incubated for 24 hours to fix the membrane. The number of cells in any five regions was counted and averaged under a microscope. Membrane fixation was performed by inhaling the medium in the upper chamber, wiping the inner cell with a cotton swab, and then in 4% formalin and incubating for 5 minutes, followed by washing with PBS. After incubation (permeabilization) for 2 minutes in Methanol, and washed with PBS. After staining for 5 minutes in Hematotoxylin (Sigma Cat # MHS16), and washed with PBS. Stain for 5 minutes in 0.5% eosin (Sigma Cat # E4382) and wash with PBS. The membrane was cut with a knife and fixed to slide glass using Canada balsam (Sigma Cat # C1795).

Experimental Example  6: Cell scratching assay

100 000 MDA-MB231 or Hs578T cells were seeded in 6-well plates. Once the cell distribution was 100% formed, it was scratched lengthwise to form a constant width. After washing twice with PBS, the medium was replaced with fresh medium and treated with the material under specified conditions after 1 hour. Cell migration was observed for 24 hours. Photographs of the cells were taken using an Inverted microscope equipped with a camera.

Experimental Example  7: Annexin  V dyeing

100 000 MDA-MB231 cells were seeded in a 6-well plate. After 24 hours, cells were treated with reagents of the indicated conditions and further incubated for 24 hours. The cells were washed with PBS, treated with Trypsin-EDTA, then detached and centrifuged at 200 × g for 5 min. Resuspend the precipitate in 100 μl of Annexin-VFLUOS labeling solution (1: 1 mixture of Annexin-V-Fluos labeling reagent (vial 1) and propidium iodide solution (vial 2) dissolved in 1 ml incubation buffer (bottle 3)). The reaction was carried out at 15-25 ° C. for 20 minutes. The flow cytometer was analyzed under 488 nm excitation, 515 nm band pass filter (for fluorescein detection) and filter <600 nm (for PI detection).

Experimental Example  8: Western blotting

100 000 MDA-MB231 or Hs578T cells were seeded in 6-well plates. After 24 hours, cells were treated with reagents of the indicated conditions and further incubated for 24 hours. Cells were washed with PBS and then centrifuged at 200 × g for 5 min. The precipitate was diluted with 100 μl of 1% Triton X-100 lysis buffer (50 mM Tris-Cl, pH 8.0, 150). mM sodium chloride, 1 mM EDTA, 1 mM EGTA, 2.5 mM sodium pyrophosphate, 1 mM sodium orthovanadate, 1 mM beta-glycerophosphate, 1 μg / ml leupeptin and 1 mM phenylmethylsulfonyl fluoride) were added and reacted at 4 ° C. for 5 minutes. After centrifugation at 2,000 rpm for 10 min, the supernatant was collected. After measuring the total protein amount by the Bradford method, the same amount of protein was separated by molecular weight through SDS-PAGE electrophoresis. The separated protein was transferred to a nitrocellulose membrane and reacted with an antibody to the protein to confirm the amount and change of the protein.

<110> UNIVERSITY-INDUSTRY COOPERATION FOUNDATION KANGWON NATIONAL UNIVERSITY <120> A COMPOSITION FOR DIAGNOSING AND TREATING CANCER COMPRISING NADPH          OXIDASE COMPLEX PROTEIN <160> 24 <170> KopatentIn 1.71 <210> 1 <211> 58 <212> DNA <213> Artificial Sequence <220> <223> human Nox1 sh oligo Fw <400> 1 ccggattctc tccagcctat ctcatctcga gatgagatag gctggagaga attttttg 58 <210> 2 <211> 58 <212> DNA <213> Artificial Sequence <220> <223> human Nox1 sh oligo Re <400> 2 aattcaaaaa attctctcca gcctatctca tctcgagatg agataggctg gagagaat 58 <210> 3 <211> 58 <212> DNA <213> Artificial Sequence <220> <223> human Nox4 sh oligo Fw <400> 3 ccggagcaag ataccgagat gaggactcga gtcctcatct cggtatcttg cttttttg 58 <210> 4 <211> 58 <212> DNA <213> Artificial Sequence <220> <223> human Nox4 sh oligo Re <400> 4 aattcaaaaa agcaagatac cgagatgagg actcgagtcc tcatctcggt atcttgct 58 <210> 5 <211> 58 <212> DNA <213> Artificial Sequence <220> <223> human Nox5 sh oligo Fw <400> 5 ccggtgttct attggactca cctgtctcga gacaggtgag tccaatagaa catttttg 58 <210> 6 <211> 58 <212> DNA <213> Artificial Sequence <220> <223> human Nox5 sh oligo Re <400> 6 aattcaaaaa tgttctattg gactcacctg tctcgagaca ggtgagtcca atagaaca 58 <210> 7 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> Human Nox1-specific primers forward <400> 7 gtacaaattc cagtgtgcag accac 25 <210> 8 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> reverse primer <400> 8 gagactggaa tatcggtgac agca 24 <210> 9 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> human Nox4-specific primers forward <400> 9 gttcttaacc tcaactgcag cc 22 <210> 10 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> reverse primer <400> 10 gcatatgtag aggctgtgat c 21 <210> 11 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> human Nox5-specific primers forward <400> 11 atgagtggca ccccttcacc atcag 25 <210> 12 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> reverse primer <400> 12 gtcagcaggc tcacaaacca ctcgaa 26 <210> 13 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> human E-cadherin-specific primers forward <400> 13 ccttcctccc aatacatctc cc 22 <210> 14 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> reverse primer <400> 14 tctccgcctc cttcttcatc 20 <210> 15 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> human Snail1-specific primers forward <400> 15 gggcaggtat ggagaggaag a 21 <210> 16 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> reverse primer <400> 16 ttcttctgcg ctactgctgc g 21 <210> 17 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> human beta actin-specific primers forward <400> 17 acgttgctat ccaggctgtg 20 <210> 18 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> reverse primer <400> 18 gcgacgtagc acagcttctc 20 <210> 19 <211> 564 <212> PRT <213> Homo Sapiens (human) NADPH OXIDASE 1 (NOX1) <400> 19 Met Gly Asn Trp Val Val Asn His Trp Phe Ser Val Leu Phe Leu Val   1 5 10 15 Val Trp Leu Gly Leu Asn Val Phe Leu Phe Val Asp Ala Phe Leu Lys              20 25 30 Tyr Glu Lys Ala Asp Lys Tyr Tyr Tyr Thr Arg Lys Ile Leu Gly Ser          35 40 45 Thr Leu Ala Cys Ala Arg Ala Ser Ala Leu Cys Leu Asn Phe Asn Ser      50 55 60 Thr Leu Ile Leu Leu Pro Val Cys Arg Asn Leu Leu Ser Phe Leu Arg  65 70 75 80 Gly Thr Cys Ser Phe Cys Ser Arg Thr Leu Arg Lys Gln Leu Asp His                  85 90 95 Asn Leu Thr Phe His Lys Leu Val Ala Tyr Met Ile Cys Leu His Thr             100 105 110 Ala Ile His Ile Ile Ala His Leu Phe Asn Phe Asp Cys Tyr Ser Arg         115 120 125 Ser Arg Gln Ala Thr Asp Gly Ser Leu Ala Ser Ile Leu Ser Ser Leu     130 135 140 Ser His Asp Glu Lys Lys Gly Gly Ser Trp Leu Asn Pro Ile Gln Ser 145 150 155 160 Arg Asn Thr Thr Val Glu Tyr Val Thr Phe Thr Ser Val Ala Gly Leu                 165 170 175 Thr Gly Val Ile Met Thr Ile Ala Leu Ile Leu Met Val Thr Ser Ala             180 185 190 Thr Glu Phe Ile Arg Arg Ser Tyr Phe Glu Val Phe Trp Tyr Thr His         195 200 205 His Leu Phe Ile Phe Tyr Ile Leu Gly Leu Gly Ile His Gly Ile Gly     210 215 220 Gly Ile Val Arg Gly Gln Thr Glu Glu Ser Met Asn Glu Ser His Pro 225 230 235 240 Arg Lys Cys Ala Glu Ser Phe Glu Met Trp Asp Asp Arg Asp Ser His                 245 250 255 Cys Arg Arg Pro Lys Phe Glu Gly His Pro Pro Glu Ser Trp Lys Trp             260 265 270 Ile Leu Ala Pro Val Ile Leu Tyr Ile Cys Glu Arg Ile Leu Arg Phe         275 280 285 Tyr Arg Ser Gln Gln Lys Val Val Ile Thr Lys Val Val Met His Pro     290 295 300 Ser Lys Val Leu Glu Leu Gln Met Asn Lys Arg Gly Phe Ser Met Glu 305 310 315 320 Val Gly Gln Tyr Ile Phe Val Asn Cys Pro Ser Ile Ser Leu Leu Glu                 325 330 335 Trp His Pro Phe Thr Leu Thr Ser Ala Pro Glu Glu Asp Phe Phe Ser             340 345 350 Ile His Ile Arg Ala Ala Gly Asp Trp Thr Glu Asn Leu Ile Arg Ala         355 360 365 Phe Glu Gln Gln Tyr Ser Pro Ile Pro Arg Ile Glu Val Asp Gly Pro     370 375 380 Phe Gly Thr Ala Ser Glu Asp Val Phe Gln Tyr Glu Val Ala Val Leu 385 390 395 400 Val Gly Ala Gly Ile Gly Val Thr Pro Phe Ala Ser Ile Leu Lys Ser                 405 410 415 Ile Trp Tyr Lys Phe Gln Cys Ala Asp His Asn Leu Lys Thr Lys Lys             420 425 430 Ile Tyr Phe Tyr Trp Ile Cys Arg Glu Thr Gly Ala Phe Ser Trp Phe         435 440 445 Asn Asn Leu Leu Thr Ser Leu Glu Gln Glu Met Glu Glu Leu Gly Lys     450 455 460 Val Gly Phe Leu Asn Tyr Arg Leu Phe Leu Thr Gly Trp Asp Ser Asn 465 470 475 480 Ile Val Gly His Ala Ala Leu Asn Phe Asp Lys Ala Thr Asp Ile Val                 485 490 495 Thr Gly Leu Lys Gln Lys Thr Ser Phe Gly Arg Pro Met Trp Asp Asn             500 505 510 Glu Phe Ser Thr Ile Ala Thr Ser His Pro Lys Ser Val Val Gly Val         515 520 525 Phe Leu Cys Gly Pro Arg Thr Leu Ala Lys Ser Leu Arg Lys Cys Cys     530 535 540 His Arg Tyr Ser Ser Leu Asp Pro Arg Lys Val Gln Phe Tyr Phe Asn 545 550 555 560 Lys Glu Asn Phe                 <210> 20 <211> 578 <212> PRT <213> Homo Sapiens (human) NADPH OXIDASE 4 (NOX4) <400> 20 Met Ala Val Ser Trp Arg Ser Trp Leu Ala Asn Glu Gly Val Lys His   1 5 10 15 Leu Cys Leu Phe Ile Trp Leu Ser Met Asn Val Leu Leu Phe Trp Lys              20 25 30 Thr Phe Leu Leu Tyr Asn Gln Gly Pro Glu Tyr His Tyr Leu His Gln          35 40 45 Met Leu Gly Leu Gly Leu Cys Leu Ser Arg Ala Ser Ala Ser Val Leu      50 55 60 Asn Leu Asn Cys Ser Leu Ile Leu Leu Pro Met Cys Arg Thr Leu Leu  65 70 75 80 Ala Tyr Leu Arg Gly Ser Gln Lys Val Pro Ser Arg Arg Thr Arg Arg                  85 90 95 Leu Leu Asp Lys Ser Arg Thr Phe His Ile Thr Cys Gly Val Thr Ile             100 105 110 Cys Ile Phe Ser Gly Val His Val Ala Ala His Leu Val Asn Ala Leu         115 120 125 Asn Phe Ser Val Asn Tyr Ser Glu Asp Phe Val Glu Leu Asn Ala Ala     130 135 140 Arg Tyr Arg Asp Glu Asp Pro Arg Lys Leu Leu Phe Thr Thr Val Pro 145 150 155 160 Gly Leu Thr Gly Val Cys Met Val Val Val Leu Phe Leu Met Ile Thr                 165 170 175 Ala Ser Thr Tyr Ala Ile Arg Val Ser Asn Tyr Asp Ile Phe Trp Tyr             180 185 190 Thr His Asn Leu Phe Phe Val Phe Tyr Met Leu Leu Thr Leu His Val         195 200 205 Ser Gly Gly Leu Leu Lys Tyr Gln Thr Asn Leu Asp Thr His Pro Pro     210 215 220 Gly Cys Ile Ser Leu Asn Arg Thr Ser Ser Gln Asn Ile Ser Leu Pro 225 230 235 240 Glu Tyr Phe Ser Glu His Phe His Glu Pro Phe Pro Glu Gly Phe Ser                 245 250 255 Lys Pro Ala Glu Phe Thr Gln His Lys Phe Val Lys Ile Cys Met Glu             260 265 270 Glu Pro Arg Phe Gln Ala Asn Phe Pro Gln Thr Trp Leu Trp Ile Ser         275 280 285 Gly Pro Leu Cys Leu Tyr Cys Ala Glu Arg Leu Tyr Arg Tyr Ile Arg     290 295 300 Ser Asn Lys Pro Val Thr Ile Ile Ser Val Ile Ser His Pro Ser Asp 305 310 315 320 Val Met Glu Ile Arg Met Val Lys Glu Asn Phe Lys Ala Arg Pro Gly                 325 330 335 Gln Tyr Ile Thr Leu His Cys Pro Ser Val Ser Ala Leu Glu Asn His             340 345 350 Pro Phe Thr Leu Thr Met Cys Pro Thr Glu Thr Lys Ala Thr Phe Gly         355 360 365 Val His Leu Lys Ile Val Gly Asp Trp Thr Glu Arg Phe Arg Asp Leu     370 375 380 Leu Leu Pro Pro Ser Ser Gln Asp Ser Glu Ile Leu Pro Phe Ile Gln 385 390 395 400 Ser Arg Asn Tyr Pro Lys Leu Tyr Ile Asp Gly Pro Phe Gly Ser Pro                 405 410 415 Phe Glu Glu Ser Leu Asn Tyr Glu Val Ser Leu Cys Val Ala Gly Gly             420 425 430 Ile Gly Val Thr Pro Phe Ala Ser Ile Leu Asn Thr Leu Leu Asp Asp         435 440 445 Trp Lys Pro Tyr Lys Leu Arg Arg Leu Tyr Phe Ile Trp Val Cys Arg     450 455 460 Asp Ile Gln Ser Phe Arg Trp Phe Ala Asp Leu Leu Cys Met Leu His 465 470 475 480 Asn Lys Phe Trp Gln Glu Asn Arg Pro Asp Tyr Val Asn Ile Gln Leu                 485 490 495 Tyr Leu Ser Gln Thr Asp Gly Ile Gln Lys Ile Ile Gly Glu Lys Tyr             500 505 510 His Ala Leu Asn Ser Arg Leu Phe Ile Gly Arg Pro Arg Trp Lys Leu         515 520 525 Leu Phe Asp Glu Ile Ala Lys Tyr Asn Arg Gly Lys Thr Val Gly Val     530 535 540 Phe Cys Cys Gly Pro Asn Ser Leu Ser Lys Thr Leu His Lys Leu Ser 545 550 555 560 Asn Gln Asn Asn Ser Tyr Gly Thr Arg Phe Glu Tyr Asn Lys Glu Ser                 565 570 575 Phe ser         <210> 21 <211> 565 <212> PRT <213> Homo sapiens (human) NADPH OXIDASE 5 (NOX5) <400> 21 Met Glu Asn Leu Thr Ile Ser Thr Ala His Trp Leu Thr Ala Pro Ala   1 5 10 15 Pro Arg Pro Arg Pro Arg Arg Pro Arg Gln Leu Thr Arg Ala Tyr Trp              20 25 30 His Asn His Arg Ser Gln Leu Phe Cys Leu Ala Thr Tyr Ala Gly Leu          35 40 45 His Val Leu Leu Phe Gly Leu Ala Ala Ser Ala His Arg Asp Leu Gly      50 55 60 Ala Ser Val Met Val Ala Lys Gly Cys Gly Gln Cys Leu Asn Phe Asp  65 70 75 80 Cys Ser Phe Ile Ala Val Leu Met Leu Arg Arg Cys Leu Thr Trp Leu                  85 90 95 Arg Ala Thr Trp Leu Ala Gln Val Leu Pro Leu Asp Gln Asn Ile Gln             100 105 110 Phe His Gln Leu Met Gly Tyr Val Val Val Gly Leu Ser Leu Val His         115 120 125 Thr Val Ala His Thr Val Asn Phe Val Leu Gln Ala Gln Ala Glu Ala     130 135 140 Ser Pro Phe Gln Phe Trp Glu Leu Leu Leu Thr Thr Arg Pro Gly Ile 145 150 155 160 Gly Trp Val His Gly Ser Ala Ser Pro Thr Gly Val Ala Leu Leu Leu                 165 170 175 Leu Leu Leu Leu Met Phe Ile Cys Ser Ser Ser Cys Ile Arg Arg Ser             180 185 190 Gly His Phe Glu Val Phe Tyr Trp Thr His Leu Ser Tyr Leu Leu Val         195 200 205 Trp Leu Leu Leu Ile Phe His Gly Pro Asn Phe Trp Lys Trp Leu Leu     210 215 220 Val Pro Gly Ile Leu Phe Phe Leu Glu Lys Ala Ile Gly Leu Ala Val 225 230 235 240 Ser Arg Met Ala Ala Val Cys Ile Met Glu Val Asn Leu Leu Pro Ser                 245 250 255 Lys Val Thr His Leu Leu Ile Lys Arg Pro Pro Phe Phe His Tyr Arg             260 265 270 Pro Gly Asp Tyr Leu Tyr Leu Asn Ile Pro Thr Ile Ala Arg Tyr Glu         275 280 285 Trp His Pro Phe Thr Ile Ser Ser Ala Pro Glu Gln Lys Asp Thr Ile     290 295 300 Trp Leu His Ile Arg Ser Gln Gly Gln Trp Thr Asn Arg Leu Tyr Glu 305 310 315 320 Ser Phe Lys Ala Ser Asp Pro Leu Gly Arg Gly Ser Lys Arg Leu Ser                 325 330 335 Arg Ser Val Thr Met Arg Lys Ser Gln Arg Ser Ser Lys Gly Ser Glu             340 345 350 Ile Leu Leu Glu Lys His Lys Phe Cys Asn Ile Lys Cys Tyr Ile Asp         355 360 365 Gly Pro Tyr Gly Thr Pro Thr Arg Arg Ile Phe Ala Ser Glu His Ala     370 375 380 Val Leu Ile Gly Ala Gly Ile Gly Ile Thr Pro Phe Ala Ser Ile Leu 385 390 395 400 Gln Ser Ile Met Tyr Arg His Gln Lys Arg Lys His Thr Cys Pro Ser                 405 410 415 Cys Gln His Ser Trp Ile Glu Gly Val Gln Asp Asn Met Lys Leu His             420 425 430 Lys Val Asp Phe Ile Trp Ile Asn Arg Asp Gln Arg Ser Phe Glu Trp         435 440 445 Phe Val Ser Leu Leu Thr Lys Leu Glu Met Asp Gln Ala Glu Glu Ala     450 455 460 Gln Tyr Gly Arg Phe Leu Glu Leu His Met Tyr Met Thr Ser Ala Leu 465 470 475 480 Gly Lys Asn Asp Met Lys Ala Ile Gly Leu Gln Met Ala Leu Asp Leu                 485 490 495 Leu Ala Asn Lys Glu Lys Lys Asp Ser Ile Thr Gly Leu Gln Thr Arg             500 505 510 Thr Gln Pro Gly Arg Pro Asp Trp Ser Lys Val Phe Gln Lys Val Ala         515 520 525 Ala Glu Lys Lys Gly Lys Val Gln Val Phe Phe Cys Gly Ser Pro Ala     530 535 540 Leu Ala Lys Val Leu Lys Gly His Cys Glu Lys Phe Gly Phe Arg Phe 545 550 555 560 Phe Gln Glu Asn Phe                 565 <210> 22 <211> 2609 <212> DNA <213> Homo Sapiens (human) NADPH OXIDASE 1 (NOX1) <400> 22 gctgatagca cagttctgtc cagagaagga aggcggaata aacttattca ttcccaggaa 60 ctcttggggt aggtgtgtgt ttttcacatc ttaaaggctc acagaccctg cgctggacaa 120 atgttccatt cctgaaggac ctctccagaa tccggattgc tgaatcttcc ctgttgccta 180 gaagggctcc aaaccacctc ttgacaatgg gaaactgggt ggttaaccac tggttttcag 240 ttttgtttct ggttgtttgg ttagggctga atgttttcct gtttgtggat gccttcctga 300 aatatgagaa ggccgacaaa tactactaca caagaaaaat ccttgggtca acattggcct 360 gtgcccgagc gtctgctctc tgcttgaatt ttaacagcac gctgatcctg cttcctgtgt 420 gtcgcaatct gctgtccttc ctgaggggca cctgctcatt ttgcagccgc acactgagaa 480 agcaattgga tcacaacctc accttccaca agctggtggc ctatatgatc tgcctacata 540 cagctattca catcattgca cacctgttta actttgactg ctatagcaga agccgacagg 600 ccacagatgg ctcccttgcc tccattctct ccagcctatc tcatgatgag aaaaaggggg 660 gttcttggct aaatcccatc cagtcccgaa acacgacagt ggagtatgtg acattcacca 720 gcgttgctgg tctcactgga gtgatcatga caatagcctt gattctcatg gtaacttcag 780 ctactgagtt catccggagg agttattttg aagtcttctg gtatactcac caccttttta 840 tcttctatat ccttggctta gggattcacg gcattggtgg aattgtccgg ggtcaaacag 900 aggagagcat gaatgagagt catcctcgca agtgtgcaga gtcttttgag atgtgggatg 960 atcgtgactc ccactgtagg cgccctaagt ttgaagggca tccccctgag tcttggaagt 1020 ggatccttgc accggtcatt ctttatatct gtgaaaggat cctccggttt taccgctccc 1080 agcagaaggt tgtgattacc aaggttgtta tgcacccatc caaagttttg gaattgcaga 1140 tgaacaagcg tggcttcagc atggaagtgg ggcagtatat ctttgttaat tgcccctcaa 1200 tctctctcct ggaatggcat ccttttactt tgacctctgc tccagaggaa gatttcttct 1260 ccattcatat ccgagcagca ggggactgga cagaaaatct cataagggct ttcgaacaac 1320 aatattcacc aattcccagg attgaagtgg atggtccctt tggcacagcc agtgaggatg 1380 ttttccagta tgaagtggct gtgctggttg gagcaggaat tggggtcacc ccctttgctt 1440 ctatcttgaa atccatctgg tacaaattcc agtgtgcaga ccacaacctc aaaacaaaaa 1500 agatctattt ctactggatc tgcagggaga caggtgcctt ttcctggttc aacaacctgt 1560 tgacttccct ggaacaggag atggaggaat taggcaaagt gggttttcta aactaccgtc 1620 tcttcctcac cggatgggac agcaatattg ttggtcatgc agcattaaac tttgacaagg 1680 ccactgacat cgtgacaggt ctgaaacaga aaacctcctt tgggagacca atgtgggaca 1740 atgagttttc tacaatagct acctcccacc ccaagtctgt agtgggagtt ttcttatgtg 1800 gccctcggac tttggcaaag agcctgcgca aatgctgtca ccgatattcc agtctggatc 1860 ctagaaaggt tcaattctac ttcaacaaag aaaatttttg agttatagga ataaggacgg 1920 taatctgcat tttgtctctt tgtatcttca gtaattgagt tataggaata aggacggtaa 1980 tctgcatttt gtctctttgt atcttcagta atttacttgg tctcntcagg tttgancagt 2040 cactttagga taagaatgtg cctctcaagc cttgactccc tggtattctt tttttgattg 2100 cattcaactt cgttacttga gcttcagcaa cttaagaact tctgaagttc ttaaagttct 2160 gaanttctta aagcccatgg atcctttctc agaaaaataa ctgtaaatct ttctggacag 2220 ccatgactgt agcaaggctt gatagcagaa gtttggtggt tcanaattat acaactaatc 2280 ccaggtgatt ttatcaattc cagtgttacc atctcctgag ttttggtttg taatcttttg 2340 tccctcccac ccccacagaa gattttaagt agggtgactt tttaaataaa aatttattga 2400 ataattaatg ataaaacata ataataaaca taaataataa acaaaattac cgagaacccc 2460 atccccatat aacaccaaca gtgtacatgt ttactgtcac ttttgatatg gtttatccag 2520 tgtgaacagc aatttattat ttttgctcat caaaaaataa aggatttttt ttcacttgaa 2580 aaaaaaaaaa aaaaaaaaaa aaaaaaaaa 2609 <210> 23 <211> 2232 <212> DNA <213> Homo Sapiens (human) NADPH OXIDASE 4 (NOX4) <400> 23 ccgcacaact gtaaccgctg ccccggccgc cgcccgctcc ttctcgggcc ggcgggcaca 60 gagcgcagcg cggcggggcc ggcggcatgg ctgtgtcctg gaggagctgg ctcgccaacg 120 aaggggttaa acacctctgc ctgttcatct ggctctccat gaatgtcctg cttttctgga 180 aaaccttctt gctgtataac caagggccag agtatcacta cctccaccag atgttggggc 240 taggattgtg tctaagcaga gcctcagcat ctgttcttaa cctcaactgc agccttatcc 300 ttttacccat gtgccgaaca ctcttggctt acctccgagg atcacagaag gttccaagca 360 ggagaaccag gagattgttg gataaaagca gaacattcca tattacctgt ggtgttacta 420 tctgtatttt ctcaggcgtg catgtggctg cccatctggt gaatgccctc aacttctcag 480 tgaattacag tgaagacttt gttgaactga atgcagcaag ataccgagat gaggatccta 540 gaaaacttct cttcacaact gttcctggcc tgacaggggt ctgcatggtg gtggtgctat 600 tcctcatgat cacagcctct acatatgcaa taagagtttc taactatgat atcttctggt 660 atactcataa cctcttcttt gtcttctaca tgctgctgac gttgcatgtt tcaggagggc 720 tgctgaagta tcaaactaat ttagataccc accctcccgg ctgcatcagt cttaaccgaa 780 ccagctctca gaatatttcc ttaccagagt atttctcaga acattttcat gaacctttcc 840 ctgaaggatt ttcaaaaccg gcagagttta cccagcacaa atttgtgaag atttgtatgg 900 aagagcccag attccaagct aattttccac agacttggct ttggatttct ggacctttgt 960 gcctgtactg tgccgaaaga ctttacaggt atatccggag caataagcca gtcaccatca 1020 tttcggtcat aagtcatccc tcagatgtca tggaaatccg aatggtcaaa gaaaatttta 1080 aagcaagacc tggtcagtat attactctac attgtcccag tgtatctgca ttagaaaatc 1140 atccatttac cctcacaatg tgtccaactg aaaccaaagc aacatttggg gttcatctta 1200 aaatagtagg agactggaca gaacgatttc gagatttact actgcctcca tctagtcaag 1260 actccgaaat tctgcccttc attcaatcta gaaattatcc caagctgtat attgatggtc 1320 cttttggaag tccatttgag gaatcactga actatgaggt cagcctctgc gtggctggag 1380 gcattggagt aactccattt gcatcaatac tcaacaccct gttggatgac tggaaaccat 1440 acaagcttag aagactatac tttatttggg tatgcagaga tatccagtcc ttccgttggt 1500 ttgcagattt actctgtatg ttgcataaca agttttggca agagaacaga cctgactatg 1560 tcaacatcca gctgtacctc agtcaaacag atgggataca gaagataatt ggagaaaaat 1620 atcatgcact gaattcaaga ctgtttatag gacgtcctcg gtggaaactt ttgtttgatg 1680 aaatagcaaa atataacaga ggaaaaacag ttggtgtttt ctgttgtgga cccaattcac 1740 tatccaagac tcttcataaa ctgagtaacc agaacaactc atatgggaca agatttgaat 1800 acaataaaga gtctttcagc tgaaaacttt tgccatgaag caggactcta aagaaggaat 1860 gagtgcaatt tctaagactt tgaaactcag cggaatcaat cagctgtgtt atgccaaaga 1920 atagtaaggt tttcttattt atgattattt gaaaatggaa atgtgagaat gtggcaacat 1980 gaccgtcaca ttacatgttt aatctggaaa ccaaagagac cctgaagaat atttgatgtg 2040 atgattcatt ttcagttctc aaattaaaag aaaactgtta gatgcacact gttgattttc 2100 atggtggatt caagaactcc ctagtgagga gctgaacttg ctcaatctaa ggctgattgt 2160 cgtgttcctc tttaaattgt ttttggttga acaaatgcaa gattgaacaa aattaaaaat 2220 tcattgaagc tg 2232 <210> 24 <211> 2223 <212> DNA <213> Homo sapiens (human) NADPH OXIDASE 5 (NOX5) <400> 24 gccgacgcgg acggcaacgg ggccatcacc ttcgaggagc tccgggacga gctgcagcgc 60 ttccccggag tcatggagaa cctgaccatc agcactgccc actggctgac ggcccccgcc 120 ccccgcccac gcccgcgccg gccgcgccag ctgacccgcg cctactggca caaccaccgc 180 agccagctgt tctgcctggc cacctatgca ggcctccacg tgctgctctt cgggctggcg 240 gccagcgcgc accgggacct cggcgccagc gtcatggtgg ccaagggctg tggccagtgc 300 ctcaacttcg actgcagctt catcgcggtg ctgatgctca gacgctgcct cacctggctg 360 cgggccacgt ggctggctca agtcctacca ctggaccaga acatccagtt ccaccagctt 420 atgggctacg tggtagtggg gctgtccctc gtgcacactg tggctcacac tgtgaacttt 480 gtactccagg ctcaggcgga ggccagccct ttccagttct gggagctgct gctcaccacg 540 aggcctggca ttggctgggt acacggttcg gcctccccga caggtgtcgc tctgctgctg 600 ctgctcctcc tcatgttcat ctgctccagt tcctgcatcc gcaggagtgg ccactttgag 660 gtgttctatt ggactcacct gtcctacctc ctcgtgtggc ttctgctcat ctttcatggg 720 cccaacttct ggaagtggct gctggtgcct ggaatcttgt ttttcctgga gaaggccatc 780 ggactggcag tgtcccgcat ggcagccgtg tgcatcatgg aagtcaacct cctcccctcc 840 aaggtcactc atctcctcat caagcggccc cctttttttc actatagacc tggtgactac 900 ttgtatctga acatccccac cattgctcgc tatgagtggc accccttcac catcagcagt 960 gctcctgagc agaaagacac tatctggctg cacattcggt cccaaggcca gtggacaaac 1020 aggctgtatg agtccttcaa ggcatcagac ccactgggcc gtggttctaa gaggctgtcg 1080 aggagtgtga caatgagaaa gagtcaaagg tcgtccaagg gctctgagat acttttggag 1140 aaacacaaat tctgtaacat caagtgctac atcgatgggc cttatgggac ccccacccgc 1200 aggatctttg cctctgagca tgccgtgctc atcggggcag gcatcggcat cacccccttt 1260 gcttccattc tgcagagtat catgtacagg caccagaaaa gaaagcatac ttgccccagc 1320 tgccagcact cctggatcga aggtgtccaa gacaacatga agctccataa ggtggacttt 1380 atctggatca acagagacca gcggtctttc gagtggtttg tgagcctgct gactaaactg 1440 gagatggacc aggccgagga ggctcaatac ggccgcttcc tggagctgca tatgtacatg 1500 acatctgcac tgggcaagaa tgacatgaag gccattggcc tgcagatggc ccttgacctc 1560 ctggccaaca aggagaagaa agactccatc acggggctgc agacgcgcac ccagcctggg 1620 cggcctgact ggagcaaggt gttccagaaa gtggctgctg agaagaaggg caaggtgcag 1680 gtcttcttct gtggctcccc agctctggcc aaggtgctga agggccattg tgagaagttc 1740 ggcttcagat ttttccaaga gaatttctag cctcacctct ccaagctctg ccccaagtcc 1800 acaccatggg tctgcttcat cgcattagta taaatgcccc cacagggacc agcctcagat 1860 gacccaccca ataagacaaa gcctagggac cccctaatcc tgctcaacag agagaacagg 1920 agaccccaag gggcagatga acttcctcta gaacccaggg gaaggggcag tgccttgttc 1980 agtctgctgt agattctggg gtttctgtga aagtgaggga accagaggct ggtcacggga 2040 gcttgggggt ggggttcgag ggggcagagg gcaaccactc ctccaaacat tttccgacgg 2100 agccttcccc cacatccatg gtcccaaacc tgcccaatca tcacagtcat ttggaagctt 2160 atttctccgg catcttataa aattgttcaa acctacagta aaaaaaaaaa aaaaaaaaaa 2220 aaa 2223

Claims (15)

A composition for diagnosing cancer comprising a polypeptide selected from the group consisting of NADPH oxidase complexes (Nox) 1, NADPH oxidase complexes (Nox) 4, and NADPH oxidase complexes (Nox) 5 and functional variants thereof as an active ingredient. According to claim 1, wherein the NADPH oxidase complex (Nox) 1, NADPH oxidase complex (Nox) 4 and NADPH oxidase complex (Nox) 5 polypeptides having the amino acid sequence of SEQ ID NO: 19, 20 and 21, respectively A cancer diagnostic composition. Cancer diagnostic composition comprising a gene selected from the group consisting of a gene encoding a NADPH oxidase complex (Nox) 1, a NADPH oxidase complex (Nox) 4 and a NADPH oxidase complex (Nox) 5 polypeptide and functional variants thereof as an active ingredient . The gene encoding the NADPH Oxidase Complex (Nox) 1, the NADPH Oxidase Complex (Nox) 4 and the NADPH Oxidase Complex (Nox) 5 polypeptides are amino acids set forth in SEQ ID NOs: 22, 23 and 24, respectively. Cancer diagnostic composition, characterized in that it has a sequence. A substrate for diagnosing cancer with a protein selected from the group consisting of NADPH Oxidase Complex (Nox) 1, NADPH Oxidase Complex (Nox) 4, and NADPH Oxidase Complex (Nox) 5 Polypeptides and functional variants thereof. 6. The NADPH oxidase complex (Nox) 1, NADPH oxidase complex (Nox) 4 and NADPH oxidase complex (Nox) 5 polypeptides have amino acid sequences set forth in SEQ ID NOs: 19, 20 and 21, respectively. Characterized by the cancer diagnostic substrate. The substrate of claim 5, wherein the substrate is nickel-PTFE (polytetrafluoroethylene), glass, apatite, silicon, gold, silver or metal. A cancer diagnostic substrate to which a gene selected from the group consisting of NADPH oxidase complex (Nox) 1, NADPH oxidase complex (Nox) 4, and NADPH oxidase complex (Nox) 5 polypeptide and functional variants thereof is attached. The gene encoding the NADPH Oxidase Complex (Nox) 1, the NADPH Oxidase Complex (Nox) 4, and the NADPH Oxidase Complex (Nox) 5 polypeptides are amino acids set forth in SEQ ID NOs: 22, 23 and 24, respectively. Cancer diagnostic substrate, characterized in that it has a sequence. 10. The substrate of claim 8 or 9, wherein the substrate is nickel-PTFE (polytetrafluoroethylene), glass, apatite, silicon, gold, silver or metal. A composition for preventing and treating cancer, comprising a therapeutically effective amount of diphenylene iodonium, derivatives thereof, or a pharmaceutically acceptable salt thereof as an active ingredient. The method of claim 11, wherein the derivative is Dibenziodium (chloride), chloride; Dibengiodium, sulfates; Dibenziodolium, 3-chloro-, butanoate; Dibengioodorium, 3-chloro-, carbonate; Dibengioodorium, 3-chloro-, 2-hydroxy-1,2,3-propanetricarboxylate (3-); Dibengioodorium, 3-chloro-, cyclopropanecarboxylate; dibengioodorium, 3-chloro-, hydroxide; Dibengioodorium, 3-chloro-, 2-hydroxyethanesulfonate; Dibenziodorium, 2-chloro-, malonate (7Cl); dibenziorium, 3-chloro-, methanesulfonate; dibenziorium, 3-chloro-, propanoate; Dibengioodorium, 3-amino-, chloride; dibengioodorium, 1-bromo-3,7-dinitro-, chloride; Dibengioodorium, 3,7-dichloro-, chloride; Dibengioodorium, 3,7-diamino-, chloride; 5H-dibengiodol, 5- (3-chlorophenyl)-(9Cl); 5H-dibengiodol, 5- (4-chlorophenyl)-( 9Cl); phenanthros [4,5-bcd] iodorium, chloride (8Cl); dibengioodorium, 1-iodo-, chloride; Dibengioodorium, 2-chloro-, phosphate; Dibengioodorium, 2,4,5-trichlorophenol salt (8Cl); dibengioodorium, 3-chloro-, sulfate; dibengioodorium, 3,7-dichloro-, sulfate; dibengioodorium , 2-chloro-, sulfate; dibengioodorium, 2,4-dichloro-, sulfate; Dibengioodorium, 3,7-dinitro-, chloride; dibengioodorium, 1- (dimethylamino) -2-naphthalenesulfonate; dibengioodorium, 4-[[(trifluoromethyl) sulphate Phenyl] oxy] benzene sulfonic acid salt; dibenziorium-2,4,6,8-t4 (9Cl); dibenziorium, chromic acid (H2Cr2O7) (9Cl) salt; dibenziorium, (OC-6 -11) -hexafluoroantimonate (1-); dibenziorium, trifluoroacetic acid (9Cl) salt; dibenziorium, trifluoromethanesulfonic acid (9Cl) salt; dibenziorium, 9,10-dihydro-9,10-dioxo-2-anthracenesulfonic acid (9Cl) salt; dibenziorium, 9,10-dimethoxy-2-anthracenesulfonic acid (9Cl) salt; dibenziodo Sodium, O-ethyl carbonodithioate (9Cl); dibenziorium, 1,1 '-[[(methyl sulfonyl) methylene] bis (sulfonyl)] bis [benzene] (9Cl) salt; Benziorium, diethylcarbamodithioate (9Cl); dibengio Donium, 1-pyrrolidinecarbodiesaoate, chloromethane (9Cl); dibenziorium, 1-pyrrolidinecarbodiesioate, trichloromethane (9Cl); dibenziorium, 1- Pyrrolidinecarbodiesaoate (9Cl); 5H-dibengiodol, 5- (2-naphthylthio)-(7Cl); 1,3-cyclohexanedione, 5,5-dimethyl-, 5H -Dibengiodol (7Cl); dibengioodorium, (T-4) -tetradomercurate (2-) (9Cl); dibengioodorium-131I (9Cl); dibengioodorium, 4-methylbenzene Sulfonic acid (9Cl) salts; dibenziorium, hexafluoroacenate (1-) (9Cl); dibenziorium, bromide; dibenziorium-5-125I (9Cl); dibenziorium, triclo Romercurate (1-) (8Cl); dibengiodorium, iodate (8Cl); dibenziorium, tetrafluoroborate (1-) (8Cl, 9Cl); dibenziorium, hexafluorophosphate (One-); Dibenziorium, citrate (8Cl); dibenziorium, 2-hydroxyethanesulfonic acid (8Cl) salt; dibenziorium, methanesulfonic acid (7Cl, 8Cl, 9Cl); dibenziorium, 2 -Hydroxyethyl sulfate (9Cl); dibengioodorium, methyl sulfate (8Cl); dibenziorium, oxonium; dibenziorium, cyclopropanecarboxylate (7Cl, 8Cl); dibenziorium, Butyrate (7Cl, 8Cl); dibengioodorium, propionate (7Cl, 8Cl); Dibengioodorium, carbonate (8Cl, 9Cl); dibengioodorium, sulfate (8Cl, 9Cl); dibengioodorium, 2,4,5-trichlorophenol (8Cl) salt; dibengioodorium, Acetates (7Cl, 8Cl); dibengioodorium, phosphate (8Cl); dibengioodorium, glycorate (7Cl, 8Cl); dibengioodorium, 2-hydroxypropanoic acid (9Cl) salt; dibengioodor Leeum, nitrate (6Cl, 7Cl, 8Cl, 9Cl); dibenziorium, iodide; Dibengioodorium, fluoride; 5H-dibengiodol (8Cl, 9Cl); dibengioodorium, 3-[[2-[(2-amino ethyl) thio] acetyl] amino]-; dibengioodorium , 3-[[2-[(2-aminoethyl) amino] acetyl] amino]-; dibengioodorium, 3-[[2-[(2-aminoethyl) thio] acetyl] amino]-, chloride , Hydrochloride; dibengioodorium, 3-[[2-[(2-aminoethyl) amino] acetyl] amino]-, chloride, hydrochloride; dibengioodorium, 3-nitro-, chloride (1: 1 Dibenziorium, 3,7-dinitro-, tetrakis (2,3,4,5,6-pentafluorophenyl) borate (1-); dibenziorium, 3-nitro-, tetra Kis (2,3,4,5,6-pentafluorophenyl) borate (1-); dibenziorium, 3-nitro-, tetraphenylborate (1-); dibenziorium, 1- ( Dimethylamino) -2-naphthalenesulfonic acid; dynaphthyl [1,2-b: 1 ', 2'-d] iodorium, tri Fluoromethanesulfonic acid salts; dynaphthyl [1,2-b: 1 ', 2'-d] iodorium, tetrakis [3,5-bis (trifluoromethyl) phenyl] borate (1-) (9Cl); dynaphthyl [2,1-b: 1 ', 2'-d] iodorium (9Cl); dibenziorium, 3-chloro-, butanoate; dibengiodol, 1-methyl -, Ion (1-); dynaphthyl [2,3-b: 2 ', 3'-d] iodorium, 1,2,3,4,8,9,10,11-octahydro; die Benziorium, 1-methoxy-; dibenziorium, 1,9-dimethoxy; dibenziorium, 3-methoxy; dibenziorium, 1,2,3,7,8,9- Hexamethoxy; dibengioodorium, 3,7-bis (methoxycarbonyl); dibengioodorium, 1- (1,3-dihydro-1,3-dioxo-2H-isoindole-2- Di)-; dibenziorium, 3,7-bis (trifluoromethyl); dibenziorium, 1-iodo; dibenziorium, 1-nitro; dibenziorium, 3-methyl; di Benziorium, 3,7-dibromo; dibenziorium, 3-chloro Carbonates; Dibengioodorium, 3-chloro-, 2-hydroxy-1,2,3-propanetricarboxylate (3-); dibengioodorium, 3-chloro-, cyclopropanecarboxylate; dibengiodo Sodium, 3-chloro-, hydroxide; dibenziorium, 3-chloro-, 2-hydroxyethanesulfonate; dibenziorium, 2-chloro-, malonate (7Cl); Dibengioodorium, 3-chloro-, methanesulfonic acid; dibengioodorium, 3-chloro-, propanoic acid; dibengioodorium, 3,7-diiodo; dibengioodorium, 1,2, 3,4-tetramethyl; dibengioodorium, 3,7-diamino; 1,20: 3,6-dimetheno-6H, 8H, 20H- [1,10] dioxacycloheneicosino [5 , 6-c] iodorium; 9,10,11,12,13,14,15,16,17,18-decahydro- (9Cl); dibenziorium, 2,3-dimethoxy; di Benziorium, 2,7-dinitro; 1,20: 3,6-dimetheno-6H, 20H- [1,4,7,10,13] pentaoxacycloheneicosino [17,18-c] iodorium, 8,9,11, 12,14,15,17,18-octahydro- (9Cl); dibenziorium, 1-phenyl; dibenziorium, 3,7-bis (trimethylammonio)-; dibenziorium, 1, 4-dimethyl; dibenziorium, 1-bromo-3,7-dinitro; dibenziorium, 4-[[(trifluoromethyl) sulfonyl] oxy] benzenesulfonic acid (9Cl) salt; Dibengioodorium, 3-amino; 1,20: 3,6-Dimetheno-6H, 8H, 20H- [1,10] dioxacycloheneicosino [5,6-c] iodorium (9Cl); 1,20: 3,6 Dimetheno-6H, 20H- [1,4,7,10,13] pentaoxacycloheneicosino [17,18-c] iodorium (9Cl); 1,20: 3,6-dimethe No-6H, 8H, 20H- [1,10] dioxacycloheneicosino [5,6-c] iodorium, 9,10,11,12,13,14,15,16,17,18- Decahydro-, iodide (9Cl); 1,20: 3,6-dimetheno-6H, 20H- [1,4,7,10,13] pentaoxacycloheneicosino [17,18-c Iodorium, 8,9,11,12,14,15,17,18-octahydro-, iodide (9Cl); dibenziorium-2,4,6,8-t4, 3-nitro -(9Cl); dibenziorium-2,4,6,8-t4 (9Cl); dynaphthyl [1,2-b: 1 ', 2'-d] iodorium, (13bS)-, Tetrafluoroborate (1-) (9Cl); dynaphthyl [1,2-b: 1 ', 2'-d] iodorium, (13bS)-(9Cl); 5H-dibengiodol-5- yl, 3- (nitro-15N)-(9Cl); 5H-dibengiodol-5-yl, 3-nitro- (9Cl); dibenziorium, 3-nitro-, sulfe Dibengioodorium, 3-amino-, chloride (1: 1); dibengioodorium, 3,3'-methylenebis-, 9,10-dimethoxy-2-anthracenesulfonic acid (9Cl) salt ; Dibenziorium, 3,3'-methylenebis- (9Cl); Dibenziorium, chromic acid (H2Cr2O7) (9Cl) salt; Dibenziorium, 1-methyl-, sulfate; Dibenziorium, 1 -Methyl; dibengioodorium, 1-methyl-, chloride; dibengioodorium, 3-methyl-, chloride; dibengioodorium, 1-bromo-3,7-dinitro-, iodide; die Benziodorium, 1-bromo-3,7-dinitro-, bromide; dibenziorium, 1-bromo-3,7-dinitro-, chloride; dibenziorium, (OC-6-11 Hexafluoroantimonate (1-); dibenziorium, trifluoroacetic acid (9Cl) salt; dibenziorium, trifluoromethanesulfonic acid (9Cl) salt; dibenziorium, 3 , 7-bis (1,1-dimethylethyl)-, 1,1,1-trifluoro Romethanesulfonic acid; dibengioodorium, 3,7-bis (1,1-dimethylethyl)-; dibengioodorium, 2-chloro-, tetrafluoroborate (1-); 5H-diben geodol , 5-[(methylsulfonyl) bis (phenylsulfonyl) methyl]-(9Cl); dibenziorium, 9,10-dihydro-9,10-dioxo-2-anthracenesulfonic acid (9Cl) salt ; Dibenziorium, 9,10-dimethoxy-2-anthracenesulfonic acid (9Cl) salt; dibenziorium, 3,7-bis (dimethylamino) -4,6-bis (hydroxymethyl)- Inner salts; dibengioodorium, O-ethyl carbonodiothioate (9Cl); dibengioodorium, 1,1 '-[[(methylsulfonyl) methylene] bis (sulfonyl) ] Bis [benzene] (9Cl) salt; dibenziorium, diethylcarbamodithioate (9Cl); dynaphthyl [2,3-b: 2 ', 3'-d] iodorium, 1 , 2,3,4,8,9,10,11-octahydro-, iodide; dynaphthyl [2,3-b: 2 ', 3'-d] iodorium, iodide; die Benziorium, 1,9-dimethoxy-, iodorium salts; dibenziorium, 1-methoxy-, iodide; 1-methyldibenziorium iodide (6Cl); dibenziodo , 3-methoxy-, iodide; dibengioodorium, 2-bromo-, iodide; dibengioodorium, 2,7-dinitro-, iodide Dibenziodorium, 3-nitro-, iodide; dibenziorium, 3-nitro-, nitrate; dibengiodonium, 1-phyllolidinecarbodithioate. Chloromethane (9Cl); dibenziorium, 1-phyllolidinecarbodithioate e, trichloromethane (9Cl); dibenziorium, 1-phyllolidinecarbodithioate (9Cl); 5H -Dibengiodol, 5- (2-naphthylthio)-(7Cl); dibengioodorium, 1,2,3,7,8,9-hexamethoxy-, iodide; 1,3- Cyclohexanedione, 5,5-dimethyl-, 5H-dibengiodol (7Cl); dibenziorium, 3,7-bis (diethylamino)-, iodide; dibengiodorium, 3,7 -Bis (ethylmethylamino)-, a formic acid composition for preventing and treating cancer. The method for preventing and treating cancer according to claim 11, wherein the cancer treatment is achieved by inhibiting cancer metastasis. The composition for preventing and treating cancer according to claim 11, wherein the therapeutically effective amount is 1 mg / kg body weight to 1000 mg / kg body weight. The composition of claim 11, wherein the composition is cisplatin, Taxol, Taxotere, Doxorubicin, Etoposide, Gleevec, Arimidex, Tamoxifen. ), Epirubicin, Adriamycin, Mitomycin-C, Flutamide, Gosereline, Gemcitabine, Megesterol Acetate acetate, Methotrexate, Dacarbazine, Amsacrine, Fluorouracil, Ifosfamide, Melphalan, Thiotepa, Carr Carboplatin, Chlorambuci, Cyclophosphamide, Vinblastine, Hydroxyurea, Nabelvine, Gemzar, Campobell ), Rufron, Eulexin, Zometa, Hycatin mtin), and Valstar (Valstar) in combination with at least one anticancer agent selected from the group consisting of cancer prevention and treatment composition.

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