KR20110040212A - Method for producing bacterial cellulose using the media containing glucuronic acid oligomer - Google Patents

Abstract

PURPOSE: A method for preparing microbial cellulose using a medium containing glucuronic acid oligomers is provided to improve water retention value and moisture content. CONSTITUTION: A microbial cellulose is prepared by culturing microbes having cellulose productivity in a medium containing glucuronic acid oligomers. The content of the glucuronic acid oligomer is 0.5-3.0%(w/v). A microbe with cellulose productivity is Acetobacter sp., Gluconacetobacter sp., Agrobacterium sp., Rhizobium sp., Pseudomonas sp., and Sarcina sp. The culture is shaking culture, stationary culture, batch culture, or continuous culture.

Description

Method for producing microbial cellulose using a medium containing glucuronic acid oligomers {Method for Producing Bacterial Cellulose Using the Media Containing Glucuronic Acid Oligomer}

The present invention relates to a method for producing microbial cellulose using a medium containing glucuronic acid oligomer, and more particularly to the microorganism in a medium containing glucuronic acid oligomer generated as a by-product when culturing a microorganism having cellulose production ability. It relates to a method for producing microbial cellulose in high yield by culturing.

Microorganisms of the genus Acetobacter capable of producing microbial cellulose can produce large amounts of cellulose secreted as extracellular fibrils, not cell wall polymers found in eukaryotes. Unlike plant-derived cellulose, microbial cellulose produced by the genus Acetobacter is newly emerging as a high value-added material as well as dietary fiber. Since microbial cellulose contains no hemicellulose, pectin, and lignin, it is easy to purify high purity cellulose in an environmentally friendly manner. In addition, microbial cellulose has a super fine mesh structure, which has a large surface area, high water retention, moldability, crystallinity, and strong tensile strength.

Microbial cellulose is being researched into practical materials such as speaker diaphragm, tourniquet, and dietary fiber due to its excellent physical properties, and because of its chemically stable and low toxicity properties, it can be applied to cosmetic pads, artificial skin, artificial blood vessels, paint or ink. It is used in thickeners and the like.

Korean Patent No. 530996 discloses a method for producing microbial cellulose using beer fermentation waste yeast, US Patent Nos. 5274199 and 4912049 disclose a method for producing acoustic diaphragm using microbial cellulose and a method for manufacturing artificial skin, respectively. Korean Patent Laid-Open Publication No. 2003-0015399 and 1997-0014612 disclose a method for preparing a dietary fiber and a beverage using microbial cellulose, respectively.

However, microbial cellulose has a high production cost despite its excellent characteristics, and the application of microbial cellulose is limited to high value-added products such as speaker diaphragms, and commercialization has not been widely made yet. Therefore, while the research for increasing the productivity of microbial cellulose continues steadily around the world, there are still many difficulties in producing microbial cellulose with high efficiency.

For example, when shaking or stirring culture to produce microbial cellulose, the strain produces microbial cellulose by transposoning the insertion sequence element in the microorganism due to shear stress generated in the incubator. switch to, but the genetic manipulation of microorganisms due to the insertion (insertion) of a gene cellulose synthesis operon (cellulose synthase operon) to occur, etc. gene region that is not yet found domain or role than the Cel ^- mutants (mutant Cel) not ^- The problem of mutants is not easily solved.

In addition, strains producing microbial cellulose produce water-soluble polysaccharides as by-products along with microbial cellulose production, which by-products adversely affect the production of microbial cellulose. For example, some acetobacter genus microorganisms produce microbial cellulose, as well as heteropolysaccharides and frucas consisting of glucose, galactose, mannose, and glucuronic acid molecules. Gluconacetobacter Gluconacetobacter produces heteropolysaccharides consisting of polyfructan, glucose, mannose, rhamnose, and glucuronic acid, which are composed of fructose. hansenii ) is known to produce glucuronic acid oligomers consisting only of glucuronic acid as a by-product during microbial cellulose production.

Therefore, the present inventors have made efforts to reduce the production of by-products during the cultivation of microorganisms having microbial cellulose production ability, and to increase the production of microbial cellulose. As a result, the present inventors have previously added a water-soluble polysaccharide as a byproduct in the medium and cultured microorganisms having microbial cellulose production ability. In this case, the production of water-soluble polysaccharides as by-products is suppressed, confirming that microbial cellulose can be produced in high yield and completing the present invention.

An object of the present invention is to provide a production method capable of producing a high yield of microbial cellulose.

Another object of the present invention is to provide a method for producing microbial cellulose with improved water retention value and water retention duration.

In order to achieve the above object, the present invention provides a method for producing microbial cellulose, characterized in that the microorganism having the ability to produce cellulose is cultured in a medium containing glucuronic acid oligomer.

In the present invention, the content of glucuronic acid oligomer contained in the medium is characterized in that 0.5 ~ 3.0% (w / v).

In the present invention, the pH of the medium containing the glucuronic acid oligomer is characterized in that 3.5 ~ 9.

In the present invention, the microorganism having the cellulose producing ability was acetonitrile bakteo (Acetobacter) in, gluconic acetonitrile bakteo (Gluconacetobacter) genus, Agrobacterium (Agrobacterium), A separation tank emptying (Rhizobium) genus Pseudomonas (Pseudomonas) in and Sar It is selected from the group consisting of the genus Sarcina , characterized in that it is preferably Gluconacetobacter hansenii PJK (KCTC 10505BP) or acetobacter xylinum ( Acetobacter xylinum ).

In the present invention, the glucuronic acid oligomer is characterized in that it acts as an inhibitor of the glucuronic acid production metabolic circuit of the microorganism having the cellulose producing ability.

In the present invention, the culture is characterized in that it is selected from the group consisting of shaking culture, stationary culture, batch culture, fed-batch culture and continuous culture.

In the present invention, the continuous culture step (a) inoculating a culture medium of a microorganism having a cellulose production capacity in a medium containing glucuronic acid oligomer (glucuronic acid oligomer); (b) culturing the microorganism in a medium containing the glucuronic acid oligomer to produce microbial cellulose; (c) inoculating a portion of the culture supernatant with fresh medium containing glucuronic acid oligomer; And (d) culturing the microorganism having the cellulose producing ability in the medium containing the glucuronic acid oligomer of step (c) to produce microbial cellulose.

In the present invention, the microbial cellulose prepared by the above production method has an initial water retention value of 100 to 106 g (water g / dry microbial cellulose g), and a water content duration of up to 22 to 25 days. It features.

According to the present invention, it is possible to produce microbial cellulose with improved water retention value and water content duration in high yield, and thus it can be widely used in industries using microbial cellulose.

In the present invention, when the water-soluble polysaccharide as a by-product is added to the medium in advance and the microorganism having the ability to produce microbial cellulose is cultured, the production of the water-soluble polysaccharide as the by-product is suppressed, and it was confirmed that the microbial cellulose can be produced with high yield. That is, microorganisms with microbial cellulose production ability produce microbial cellulose in culture and water-soluble polysaccharides such as glucuronic acid oligomers as by-products, so that the production of microbial cellulose and glucuronic acid oligomers is mutually different in the metabolic pathway of microorganisms. As it is offset, it is predicted that adding a certain amount of by-product glucuronic acid oligomer to the medium to manipulate the metabolic circuit will increase the production of microbial cellulose without producing any by-products.

In one embodiment of the present invention, Gluconacetobacter hansenii and Acetobacter xylinum were incubated in a medium containing glucuronic acid oligomer under various pH conditions and culture methods. As a result, when the content and pH of the glucuronic acid oligomer is satisfied with certain conditions, it was confirmed that the production amount of microbial cellulose was increased.

Therefore, in one aspect, the present invention relates to a method for producing microbial cellulose, wherein the microorganism having the ability to produce cellulose is cultured in a medium containing glucuronic acid oligomer.

In the present invention, the content of glucuronic acid oligomer contained in the medium is characterized in that 0.5 ~ 3.0% (w / v). When the content of the glucuronic acid oligomer is less than 0.5% (w / v), it is difficult to suppress the production of glucuronic acid oligomer during microbial cellulose production, and exceeds 3.0% (w / v). In this case, it may have an inhibitory effect on the production metabolism of microbial cellulose.

In addition, in the present invention, the pH of the medium containing the glucuronic acid oligomer is preferably 3.5-9. If the pH of the medium is less than 3.5 but the pH exceeds 9, there is a problem in that the survival of the bacteria is difficult.

The glucuronic acid oligomer contained in the medium may be one obtained by culturing a microorganism having glucuronic acid oligomer generating ability or microbial cellulose generating ability, and the glucuronic acid oligomer may be a monomer or a dimer. It is preferable to use dimers, trimers, tetramers, pentamers, and hexamers.

The glucuronic acid oligomer is characterized in that it acts as an inhibitor of the glucuronic acid production metabolic circuit of the microorganism having the ability to produce cellulose in culture. That is, in the microorganisms having the ability to produce microbial cellulose, the production of microbial cellulose and glucuronic acid oligomers is offset against each other on a metabolic pathway, so that the glucuronic acid oligomers previously added to the medium are inhibitors of glucuronic acid oligomer production. As a result, the glucuronic acid oligomer is no longer produced.

In the present invention, the glucuronic acid-containing medium may include glucose, yeast extract, peptone, succinic acid, acetic acid, ethanol and the like in addition to glucuronic acid.

In the present invention, the microorganism having the cellulose producing ability was acetonitrile bakteo (Acetobacter) in, gluconic acetonitrile bakteo (Gluconacetobacter) genus, Agrobacterium (Agrobacterium), A separation tank emptying (Rhizobium) genus Pseudomonas (Pseudomonas) in, Sar The genus Sacina may be exemplified, and preferably Gluconacetobacter hansenii PJK (KCTC 10505BP), Acetobacter xylinum ATCC23769.

In the present invention, the microorganism having the ability to produce cellulose can be cultured by shaking culture, stationary culture, batch culture, fed-batch culture, continuous culture and the like.

The shaking culture is a method of culturing while shaking the culture solution inoculated with the microorganism, the stationary culture means a method of culturing without leaving the liquid culture solution inoculated with the microorganism without shaking, the batch culture is fixed the volume of the culture medium It refers to a method of culturing without adding a new culture solution from the outside, and the oil value culture refers to a single batch in which all of the raw materials are initially put in a culture tank and cultured. It refers to a method of culturing by adding a small amount of raw materials, the continuous culture means a culture method in which a new nutrient medium is continuously supplied and at the same time the culture solution containing cells and products is continuously removed.

The culture is preferably performed for 5 to 15 days at 25 ~ 35 ℃ to produce the optimal microbial cellulose.

The continuous culturing comprises the steps of: (a) inoculating a culture medium of a microorganism having cellulose producing ability into a medium containing glucuronic acid oligomer; (b) culturing the microorganism in a medium containing the glucuronic acid oligomer to produce microbial cellulose; (c) inoculating a portion of the culture supernatant with fresh medium containing glucuronic acid oligomer; And (d) culturing the microorganism having the cellulose producing ability in the medium containing the glucuronic acid oligomer of step (c) to produce microbial cellulose. The continuous culture may be carried out by repeating the steps (a) to (b) or (c) to (d).

In the present invention, the microbial cellulose produced through the culture can be separated and purified by reacting for 15 minutes at 121 ℃, 1.5 atm under 0.3N NaOH solution, after drying in the culture solution, the glucuronic acid is added to the remaining culture solution After adding 5 times the volume of ethanol and reacting at 4 ° C. for 2 hours, the process of centrifugation may be repeated three times.

The microbial cellulose prepared by the above method is characterized in that the initial water retention value (water retention value) is 100 ~ 108g (moisture g / dry microbial cellulose g), the water content duration is 22 to 25 days.

When the moisture content of the microbial cellulose is high and the moisture content is long, it is possible to increase the dosage of the medicine or the lotion in the manufacture of the medical wet sheet or the cosmetic mask pack as well as to provide a long time continuous supply.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

Example 1: Gluconate oligomer production in fed-batch culture using gluconacetobacter Hanseni PJK

The medium solution of the composition shown in Table 1 below was added to an Erlenmeyer flask, and sterilized at 121 ° C. for 15 minutes. Gluconacetobacter hansenii PJK in sterile medium (KCTC 10505 BP) was inoculated, incubated at 30 ° C. for 24 hours, and then 100 ml of the culture solution was inoculated into 3 L of sterile fresh medium solution. The pH of the solution was adjusted to 6, and cultured for 10 days by a fed batch method in which 100 ml of medium was added every 12 hours. As a result of the culture, it was possible to produce a dry weight 60 g glucuronic acid oligomer in 10 days culture.

TABLE 1

ingredient Volume (/ L) Glucose (SIGMA, USA)
Yeast Extract (DIFCO, USA)
Peptone (DIFCO, USA)
Succinic Acid (WAKO, Japan)
Acetic acid (Dongyang Chemical, Korea)
Ethanol (Dongyang Chemical, Korea) 10 g
10 g
7 g
0.2 g
1.5ml
1ml

Example 2: Stationary culture using Gluconacetobacter Hanseni PJK in medium (pH5) with or without glucuronic acid oligomer

The medium solution of the composition shown in Table 1 was put into an Erlenmeyer flask, and sterilized for 15 minutes at 121 ℃. Gluconacetobacter hansenii PJK (KCTC 10505 BP) was inoculated into sterilized medium, and cultured at 30 ° C. for 24 hours, and then 20 ml of the culture solution was prepared in Example 1 in the medium composition of Table 1 above. 1 L of medium solution to which 10 g (1% (w / v)) of glucuronic acid oligomer was added and 1 L of medium solution to which no glucuronic acid oligomer was added were inoculated, and the cells were incubated at pH 5 and 30 ° C.

5, 10, and 15 days after the incubation, the number of microorganisms and the amount of microbial cellulose produced from the culture medium were confirmed and shown in Table 2.

At 10 days of culture, the microbial CFU (1.2 × 10 ⁵ ) in the culture medium containing 10 g of glucuronic acid oligomer was 1.62 times higher than the microbial CFU (7.4 × 10 ⁴ ) in the culture medium without glucuronic acid oligomer.

TABLE 2

Dry weight of microbial cellulose produced in culture solution containing 1% (w / v) glucuronic acid oligomer (g) Dry weight (g) of microbial cellulose produced in culture medium without glucuronic acid oligomer added 1% (w / v) 5-day culture 10 days culture 15 days culture 5-day culture 10 days culture 15 days culture 5.15 7.77 13.31 2.03 4.44 8.74

As shown in Table 2, the production of microbial cellulose was increased according to the number of culture days up to 15 days of culture, and the microbial cellulose production harvested from the culture solution containing 10 g of glucuronic acid oligomer was not added to the glucuronic acid oligomer on day 15 of the culture. It was confirmed that 1.52 times more than the amount produced in the culture.

Example 3: Stationary culture with acetobacter xylium in medium (pH5) with or without glucuronic acid oligomer

The culture was carried out in the same manner as in Example 2 except that Acetobacter xylinum (ATCC23769) was used instead of Gluconacetobacter Hanseni PJK (KCTC 10505 BP).

The number of microorganisms and the amount of microbial cellulose produced from the culture solution was confirmed on the 5th, 10th, and 15th days, respectively, and the results are shown in Table 3.

The microbial CFU (2.9 × 10 ⁶ ) in the culture medium containing 10 g of glucuronic acid oligomer was 2.64 times higher than the microbial CFU (1.1 × 10 ⁶ ) in the culture medium without glucuronic acid oligomer.

[Table 3]

Dry weight of microbial cellulose produced in culture solution containing 1% (w / v) glucuronic acid oligomer (g) Dry weight (g) of microbial cellulose produced in culture medium without glucuronic acid oligomer added 1% (w / v) 5-day culture 10 days culture 15 days culture 5-day culture 10 days culture 15 days culture 2.98 4.80 9.86 1.87 2.99 6.28

As shown in Table 3, the production of microbial cellulose was increased according to the number of culture days up to 15 days of culture, and the microbial cellulose production harvested from the culture solution containing 10 g of glucuronic acid oligomer was not added to the glucuronic acid oligomer on day 15 of the culture. 1.57 times more than the amount produced in the culture was confirmed.

Example 4 Shaking and Static Culture Using Gluconacetobacter Hanseni PJK in Medium (pH5) Added Glucuronic Acid Oligomer

The medium solution of the composition shown in Table 1 was put into an Erlenmeyer flask, and sterilized for 15 minutes at 121 ℃. Gluconacetobacter hansenii PJK (KCTC 10505 BP) was inoculated into sterilized medium, and cultured at 30 ° C. for 24 hours, and then 20 ml of the culture solution was prepared in Example 1 in the medium composition of Table 1 above. 10 g (1% (w / v)) of glucuronic acid oligomer was inoculated into 1 L of the medium solution, followed by shaking and stationary culture at pH5. The shaking culture was incubated using a shaking incubator while rotating at 30 ° C. at a speed of 150 rpm, and the stationary culture was incubated at 30 ° C. The amount of microbial cellulose produced from the culture solution was confirmed at 5 days, 10 days, and 15 days after the culture, and is shown in Table 4.

[Table 4]

Dry weight of microbial cellulose produced in culture solution containing 1% (w / v) glucuronic acid oligomer (g) Culture method 5-day culture 10 days culture 15 days culture concussion 2.68 3.14 4.58 politics 5.15 7.77 13.31

 As shown in Table 4, the production of microbial cellulose was increased according to the number of culture days up to 15 days of culture, and it was confirmed that the microbial cellulose production harvested from the stationary culture on the 15th day of culture was 2.9 times more than that produced in shaking culture.

Example 5: Shaking and Static Culture with Acetobacter Xylium in Medium (pH5) Added with Glucuronic Acid Oligomer

Shaking and political culture were carried out in the same manner as in Example 4 except that Acetobacter xylinum (ATCC23769) was used instead of Gluconacetobacter Hanseni PJK (KCTC 10505 BP).

The amount of microbial cellulose produced from the culture solution was confirmed at 5 days, 10 days, and 15 days after the culture, and is shown in Table 5.

TABLE 5

Dry weight of microbial cellulose produced in culture solution containing 1% (w / v) glucuronic acid oligomer (g) Culture method 5-day culture 10 days culture 15 days culture concussion 1.28 4.17 4.25 politics 2.98 4.80 9.86

As shown in Table 5, the production of microbial cellulose was increased according to the number of culture days until the 15 days of culture, it was confirmed that the microbial cellulose production harvested from the stationary culture on the 15th day of culture 2.3 times more than the amount produced in shaking culture.

Example 6: Shaking and Stationary Culture Using Gluconacetobacter Hanseni PJK and Acetobacter Xylium in Medium (pH3.5) Added with Glucuronic Acid Oligomer

Gluconacetobacter hansenii PJK and acetobacter xylinum in the same manner as in Examples 4 and 5, except that the pH of the medium to which the glucuronic acid oligomer was added was 3.5 instead of 5. Shake and stirred cultures were performed using the strain (ATCC23769), respectively.

The amount of microbial cellulose produced from the culture solution was confirmed at 5 days, 10 days, and 15 days after the culture, and is shown in Table 6.

TABLE 6

Dry weight of microbial cellulose produced in culture solution containing 1% (w / v) glucuronic acid oligomer (g) Culture method
Gluconacetobacter
hansenii PJK Acetobacter xylinum 5 days
culture 10 days culture 15 days culture 5 days
culture 10 days
culture 15 days culture concussion 2.31 2.57 2.88 0.72 2.23 2.88 politics 4.58 6.17 10.30 9.86 10.0 15.3

As shown in Table 6, it was confirmed that the production of microbial cellulose by Acetobacter xylinum was higher than that by Gluconacetobacter hansenii PJK, unlike the results of culture at pH 3.5.

Experimental Example 1 Measurement of Water Content and Water Content Duration of Microbial Cellulose Produced by Gluconacetobacter Hanseni PJK in a Medium Without or Without Glucuronic Acid Oligomer

After harvesting the microbial cellulose harvested on the 10th day of static culture in pH5 medium to which 1% (w / v) glucuronic acid oligomer was added as in Example 2, and removing the cells, the wet weight of the pure microbial cellulose was measured, and 23 ° C. The dry weight of the microbial cellulose was measured by drying until there was no change in weight.

The moisture content of the microbial cellulose was determined by subtracting the dry weight from the initial wet weight of the microbial cellulose, and the water content per dry weight of the microbial cellulose was measured by dividing the moisture content by the dry weight of the microbial cellulose. Indicated.

TABLE 7

Hours Microbial Cellulose Water Content
(Moisture g / dry microbial cellulose g) Add glucuronic acid oligomer
In uncultured culture
Acid Microbial Cellulose Add glucuronic acid oligomer
Rice produced in broth
Biological cellulose 0 88.0 106.0 2 70.2 95.8 4 45.2 85.5 7 25.0 65.7 10 1.8 48.9 13 0.0 26.5 16 0.0 14.0 19 0.0 6.43 22 0.0 1.3 25 0.0 0.0 28 0.0 0.0 31 0.0 0.0

As shown in Table 7, it can be seen that the water content of the microbial cellulose produced in the stationary culture to which the glucuronic acid oligomer was added was 1.2 times higher than the water content of the microbial cellulose produced by the stationary culture without the addition of the glucuronic acid oligomer. there was.

In addition, while the water-containing persistence of the microbial cellulose produced by the static culture in the medium containing glucuronic acid oligomer was 25 days, the water-containing persistence of the microbial cellulose produced in the medium without the glucuronic acid oligomer was 13 days. Only confirmed.

Experimental Example 2 Measurement of Changes in Microbial Cellulose Production and Glucuronic Acid Oligomer Consumption of Gluconacetobacter Hanseni PJK According to the Glucuronic Acid Oligomer Added in the Medium

Gluconacetobacter hansenii ( Gluconacetobacter hansenii ) in the medium solution to which 5 g (0.5% (w / v)) to 40 g (4% (w / v)) of glucuronic acid oligomer was added to 1 L of the medium of Table 1 above. After inoculating 20 ml of PJK, shaking and standing culture were carried out in the same manner as in Example 4 for 5 days at pH5.

After incubation, the microbial cellulose and glucuronic acid oligomers produced in each medium culture were separated, and their amounts were measured and shown in Table 8.

[Table 8]

Glucuronic acid
Oligomer
Amount (g) Culture method Harvested after 5 days incubation
Microbial cellulose
Dry weight (g) After 5 days incubation
Glucuronic Acid Oligomer Dry Weight (g) 0 concussion 0.90 0.30 5 concussion 1.53 3.15 10 concussion 3.86 7.45 20 concussion 4.36 16.35 30 concussion 1.52 30.35 40 concussion 0.02 40.75 0 politics 4.79 0.1 5 politics 6.00 2.90 10 politics 7.59 9.00 20 politics 10.30 18.20 30 politics 0.40 30.00 40 politics 0.04 41.50

As shown in Table 8, the production of microbial cellulose increased 1.7-4.8 times depending on the amount of glucuronic acid oligomer added up to 30 g in shaking culture, and some of the glucuronic acid oligomer was consumed when the glucuronic acid oligomer was added up to 20 g. Able to know. In stationary culture, as the amount of glucuronic acid oligomer added increased to 20g, the production of microbial cellulose increased 1.25 ~ 2.15 times, indicating that some of the glucuronic acid oligomer was consumed.

However, in the shaking culture, it was confirmed that when the amount of the glucuronic acid oligomer added is 40 g or more, the yield of the microbial cellulose is drastically reduced and almost no microbial cellulose is produced. In addition, it was confirmed that in stationary culture, when the amount of glucuronic acid oligomer added is 30 g or more, almost no microbial cellulose is produced.

Experimental Example 3: Change of strain activity (CFU) of acetic acid bacteria and survival range of bacteria according to pH

Microorganisms CFU measured after incubation for 5 days at various pH (2.5 ~ 9.5) by inoculating Acetobacter xylinum (ATCC23769) in the same manner as in Example 2 is shown in Table 9.

TABLE 9

pH 2.5 3.5 5.0 7.0 9.0 9.5 CFU 0 1.5 × 10 ⁵ 4.3 × 10 ⁴ 1.7 × 10 ⁴ 1.6 × 10 ² 0

As shown in Table 9, the microbial CFU measured in the static culture added with glucuronic acid oligomer was measured high between pH 3.5 ~ 9.0 and was not measured below pH 3.5 and above pH 9.0. In other words, in order to survive and produce microbial cellulose, it was confirmed that the culture must be in between pH 3.5 ~ 9.0.

As described above in detail the specific parts of the present invention, it is apparent to those skilled in the art that such specific description is merely a preferred embodiment, thereby not limiting the scope of the present invention. something to do. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (9)

A method for producing microbial cellulose, comprising culturing a microorganism having cellulose-producing ability in a medium containing glucuronic acid oligomer. The method of claim 1, wherein the content of glucuronic acid oligomer contained in the medium is 0.5 to 3.0% (w / v). The method according to claim 1, wherein the pH of the medium containing glucuronic acid oligomer is 3.5-9. The microorganism of claim 1, wherein the microorganism having cellulose- producing ability is genus Acetobacter, genus Gluconacetobacter , genus Agrobacterium , genus Rhizobium , genus Pseudomonas , and Method selected from the group consisting of Sarcina genus. The method of claim 4, wherein the microorganism having cellulose- producing ability is Gluconacetobacter hansenii PJK (KCTC 10505BP) or Acetobacter xylinum . The method of claim 1, wherein the glucuronic acid oligomer acts as an inhibitor of the glucuronic acid production metabolic circuit of the microorganism having the ability to produce cellulose. The method of claim 1, wherein the culture is selected from the group consisting of shaking culture, stationary culture, batch culture, fed-batch culture and continuous culture. The method of claim 7, wherein the continuous culture comprises the following steps: (a) inoculating a culture medium of a microorganism having cellulose producing ability into a medium containing glucuronic acid oligomer; (b) culturing the microorganism in a medium containing the glucuronic acid oligomer to produce microbial cellulose; (c) inoculating a portion of the culture supernatant with fresh medium containing glucuronic acid oligomer; And (d) culturing the microorganism having the cellulose producing ability in the medium containing the glucuronic acid oligomer of step (c) to produce microbial cellulose. The method according to any one of claims 1 to 8, wherein the microbial cellulose has an initial water retention value of 100 to 106 g (g / min / dry microbial cellulose g) and a water content duration of up to 22 to 25 days. Characterized in that the method.