KR20110039181A - Method of detecting antigen, apparatus and micro fluidic chip for detecting antigen using the same - Google Patents

Method of detecting antigen, apparatus and micro fluidic chip for detecting antigen using the same Download PDF

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KR20110039181A
KR20110039181A KR1020100083629A KR20100083629A KR20110039181A KR 20110039181 A KR20110039181 A KR 20110039181A KR 1020100083629 A KR1020100083629 A KR 1020100083629A KR 20100083629 A KR20100083629 A KR 20100083629A KR 20110039181 A KR20110039181 A KR 20110039181A
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antibody
antigen
nanobeads
nanobead
detection
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KR1020100083629A
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Korean (ko)
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명남수
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한국전자통신연구원
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Priority to JP2010228783A priority patent/JP2011080999A/en
Publication of KR20110039181A publication Critical patent/KR20110039181A/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82B—NANOSTRUCTURES FORMED BY MANIPULATION OF INDIVIDUAL ATOMS, MOLECULES, OR LIMITED COLLECTIONS OF ATOMS OR MOLECULES AS DISCRETE UNITS; MANUFACTURE OR TREATMENT THEREOF
    • B82B3/00—Manufacture or treatment of nanostructures by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units
    • B82B3/0009—Forming specific nanostructures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors

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Abstract

An antigen detection method, an antigen detection apparatus and a microfluidic chip using the same are provided. Antigen detection method comprises the steps of combining the first antibody and nanobeads (nano bead) to produce antibody nanobeads, combining the generated antibody nanobeads and antigens to generate antigen antibody nanobeads, generated antigen antibody nanobeads Forming at least one of an electric field and a magnetic field in the antigen antibody nanobeads generated by combining the second antibody and the step of detecting the antigen antibody nanobead bound to the second antibody. Therefore, when the nanobeads affected by the electromagnetic field exist inside the electromagnetic field that changes in time and space, the nanobeads are moved according to the nonuniformity of the electromagnetic field. In particular, active mixing can be performed using spatially non-uniform and temporally varying electromagnetic fields, reducing reaction time and controlling flow to move nanobeads to the presence of capture antibodies. Thus, the antigen can be detected with a small amount of blood in a short time.

Description

Antigen detection method, antigen detection apparatus and microfluidic chip using the same {Method of Detecting Antigen, Apparatus and Micro Fluidic Chip for Detecting Antigen using the same}

Antigen detection method, antigen detection device and microfluidic chip using the same Relates to a fluid chip.

We look at general methods for detecting the presence of specific antigens in a sample. First, an antibody fluorescent nanobead is prepared by attaching an antibody that selectively binds to a specific antigen to nanobeads having fluorescent components. After mixing the sample to test whether a specific antigen is present and the antibody fluorescence nanobeads in a certain ratio, when the reaction of a specific antigen and the antibody occurs, the antigen antibody fluorescence nanobead combined with the antibody is made. As a result, when antigenic antibody fluorescent nanobeads are detected, it can be seen that a specific antigen is present in the sample.

In general, a biosensor using micro fluidics sends a small amount of sample to a mixing chamber through a fluorescent antibody nanobead.

Antigen antibody fluorescent nanobeads that have passed through the mixing zone pass through a detection chamber on the flow path of the fluid. The detection region includes a region in which an antibody is attached to a wall of a fluid channel.

Antibody Fluorescent Fluorescent Antibody Fluorescent Antibody Fluorescent Antibodies Attached to an Antibody Attached to Fluorescent Nanobeads The surface of an antibody antibody is fixed to the path wall of a fluid and the antigen antibody fluorescent nanofluorides binds to the surface of the nanobeads. Nanobeads will adhere to the surface of the detection area.

After sufficient reaction time has elapsed in the detection chamber, the antibody nanobeads that have not reacted (not attached to the antibody immobilized on the path surface) are washed out. When light is irradiated to the detection area from the outside, fluorescence is generated in the immobilized antibody fluorescence nanobead through the reaction between the selective antigen and the antibody.

In other words, the biosensor detects the fluorescence, detects the antigen using a small amount of the sample, and can also measure the antigen concentration in the sample.

On the other hand, antigen antibody fluorescence nanobeads are colloidal (Brownian Movement) in the fluid and drifted in the fluid, so that natural binding can occur, but antigen antibody fluorescence nanobeads and antibodies should be located close enough to the antigen And the antibody may be bound. That is, sufficient time is required for the antigen-binding fluorescent nanobead to which the antigen is bound reacts with the antibody fixed on the surface.

In addition, when a fluid flows in a channel, the velocity of the fluid flow becomes zero at the channel wall and a large colloid due to a laminar flow phenomenon that has a maximum velocity at the center of the channel. The particles are mainly concentrated in the center of the channel and move with the fluid. In other words, the antigen antibody fluorescent nanobeads that are collected at the center of the channel should be approached to the channel wall where the fixed antigen is located so that the antigen and the antibody can be bound.

As a result, when the fluid is stopped and the binding of the antibody and the antigenic antibody fluorescent nanobeads on the channel wall depends only on Brownian motion, it takes a long time, and when the fluid flows, laminar flow ), The antigenic antibody fluorescent nanobeads are likely to flow down the center of the channel. That is, since the probability of reaction between the antigen antibody fluorescence nanobead and the immobilized antibody decreases, it takes a long time and requires a large amount of the sample.

That is, the prior art has a problem of providing a sufficient time and a sufficient amount to allow the necessary binding reaction between the antigen and the antibody fluorescent nanobead in the sample, and the necessary binding reaction between the fixed antibody and antigen antibody nanobead can occur There is a problem of providing sufficient time and sufficient amount.

An object of the present invention is to provide an antigen detection method capable of efficiently detecting an antigen by forming an electromagnetic field that is spatially nonuniform and changes in time.

Another object of the present invention is to provide an antigen detecting apparatus using an antigen detecting method capable of efficiently detecting antigen by forming a spatially nonuniform and temporally varying electromagnetic field.

It is still another object of the present invention to provide a microfluidic chip using an antigen detection method capable of efficiently detecting antigens by forming an electromagnetic field that is spatially nonuniform and changes in time.

Antigen detection method for achieving the above object of the present invention comprises the steps of combining the first antibody and nanobeads (nano bead) to produce antibody nanobeads, by combining the generated antibody nanobeads and antigen antigen antigen nanobeads Generating an at least one of an electric field and a magnetic field in the generated antigen antibody nanobead, combining the generated antigen antibody nanobead with a second antibody and the antigen antibody nanobead bound to the second antibody; It can be configured to include a step of detecting.

Here, the nanobead may be configured to include at least one of a dielectric and a metal.

Here, the dielectric may have a dipole moment.

Here, the nanobead may be configured to include a fluorescence component.

Here, the generating of the antigen antibody nanobead may be to form at least one of an electric field and a magnetic field in the antibody nanobead.

Here, at least one of the electric field and the magnetic field formed in the step of combining the generated antigenic antibody nanobead and the second antibody may be non-uniform.

Here, the second antibody may be attached to a fixed position in the step of combining the generated antigen antibody nanobead and the second antibody.

An antigen detection apparatus using the antigen detection method for achieving the above object of the present invention is a mixed region for generating an antigen antibody nanobead by combining the antibody nanobead formed of the first antibody and nanobead (nano bead) with the antigen, It may include a detection region for coupling the antigen antibody nanobead and the second antibody and an electromagnetic field generator for forming at least one of an electric field and a magnetic field in at least one of the mixed region and the detection region.

Here, the nanobead may be configured to include at least one of a dielectric and a metal.

Here, the dielectric may have a dipole moment.

Here, the nanobead may be configured to include a fluorescence component.

Here, at least one of the electric field and the magnetic field of the electromagnetic field generating unit may be nonuniform.

Here, the second antibody may be attached to the detection area.

The microfluidic chip using the antigen detection method for achieving the above object of the present invention is a mixed region for generating antigen antibody nanobeads by combining the antibody nanobeads formed of the first antibody and nanobeads with the antigen, It may include a detection region for coupling the antigen antibody nanobead and the second antibody and an electromagnetic field generator for forming at least one of an electric field and a magnetic field in at least one of the mixed region and the detection region.

Here, the nanobead may be configured to include at least one of a dielectric and a metal.

Here, the dielectric may have a dipole moment.

Here, the nanobead may be configured to include a fluorescence component.

Here, the second antibody may be attached to the detection area.

Here, at least one of the electric field and the magnetic field of the electromagnetic field generating unit may be nonuniform.

The electromagnetic field generating unit may be formed of at least one of an electrode array and a micro coil array.

According to the antigen detection method as described above, the antigen detection device and the microfluidic chip using the same, if the nanobeads affected by the electromagnetic field is present inside the electromagnetic field that changes in time and space, the movement according to the nonuniformity of the electromagnetic field. In particular, active mixing can be performed using spatially non-uniform and temporally varying electromagnetic fields, reducing reaction time and controlling flow to move nanobeads to the presence of capture antibodies. Thus, the antigen can be detected with a small amount of blood in a short time.

1 is a flowchart illustrating an antigen detection method according to an embodiment of the present invention.
2 is a block diagram illustrating an antigen detection apparatus using an antigen detection method according to an embodiment of the present invention.
3 is a conceptual diagram illustrating a microfluidic chip using an antigen detection method according to an embodiment of the present invention.

As the present invention allows for various changes and numerous embodiments, particular embodiments will be illustrated in the drawings and described in detail in the written description.

However, this is not intended to limit the present invention to specific embodiments, it should be understood to include all modifications, equivalents, and substitutes included in the spirit and scope of the present invention.

The terms first, second, etc. may be used to describe various components, but the components should not be limited by the terms. The terms are used only for the purpose of distinguishing one component from another. For example, without departing from the scope of the present invention, the first component may be referred to as the second component, and similarly, the second component may also be referred to as the first component. And / or < / RTI > includes any combination of a plurality of related listed items or any of a plurality of related listed items.

When a component is referred to as being "connected" or "connected" to another component, it may be directly connected to or connected to that other component, but it may be understood that other components may be present in between. Should be. On the other hand, when a component is said to be "directly connected" or "directly connected" to another component, it should be understood that there is no other component in between.

The terminology used herein is for the purpose of describing particular example embodiments only and is not intended to be limiting of the present invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. In this application, the terms "comprise" or "have" are intended to indicate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, and one or more other features. It is to be understood that the present invention does not exclude the possibility of the presence or the addition of numbers, steps, operations, components, components, or a combination thereof.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art. Terms such as those defined in the commonly used dictionaries should be construed as having meanings consistent with the meanings in the context of the related art, and shall not be construed in ideal or excessively formal meanings unless expressly defined in this application. Do not.

Hereinafter, with reference to the accompanying drawings, it will be described in detail a preferred embodiment of the present invention. In the following description of the present invention, the same reference numerals are used for the same elements in the drawings and redundant descriptions of the same elements will be omitted.

1 is a flowchart illustrating an antigen detection method according to an embodiment of the present invention.

Referring to Figure 1, the antigen detection method according to an embodiment of the present invention is to combine the first antibody and nano beads (nano bead) to generate antibody nanobeads (S110), the generated antibody nanobeads and antigen Generating an antigen antibody nanobead by combining (S120), forming at least one of an electric field and a magnetic field on the generated antigen antibody nanobead, and combining the generated antigenic antibody nanobead and the second antibody (S130). And detecting the antigen antibody nanobead bound to the second antibody (S140).

First, the nanobead may be configured to include at least one of a dielectric and a metal, and the dielectric may have a dipole moment.

When the nanobead is configured to include a dielectric or a metal, it is subjected to a force under an electric field and a magnetic field, and thus moves within a non-uniform electric field and a magnetic field. That is, the nanobeads can be controlled to perform the desired movement while maintaining the electric and magnetic fields unevenly.

For example, surface coating of a dielectric or metal component on the nanobeads causes a force in an uneven electric and magnetic field, and controlling the non-uniform electric and magnetic fields controls the nanobead to move to a desired position. You can do it.

In addition, the nanobead may be configured to include a fluorescence component. In the case where the nanobead comprises a fluorescent component, it can be relatively simply processed to determine whether the antigen is present.

That is, by irradiating light of the wavelength band to which the fluorescent component reacts, it is possible to easily know whether the antigen is present in the sample. That is, when the fluorescent component reacts to the irradiated light, it may be determined that a specific antigen is present in the sample.

Next, the step of producing the antibody nanobeads by combining the first antibody and nanobeads (nano bead) (S110) may be referred to as a preparation for determining the presence of the antigen in the sample. That is, to determine the presence of the antigen is to prepare an antibody capable of binding to the antigen.

In addition, in order to easily determine the presence of the antigen in the final step is to prepare the nanobeads in combination with the antibody in advance. Thus, using the nanobead bound to the antibody, it is possible to force the movement of the nanobead or to make the nanobead fluorescing upon detection of the antigen.

Next, the step of generating the antigen antibody nanobead by combining the generated antibody nanobead and the antigen (S120) is to combine the antigen and the antibody nanobead in the sample to determine whether the antigen is present in the sample. The binding produces antigen antibodies nanobeads.

That is, if the antigen is not present in the sample, the antigen antibody nanobeads will not be generated, so it will not be possible to detect the antigen in a later step, but if the antigen is present in the sample, the antigen antibody nanobead is generated by the binding. The antigen may be detected at a later stage.

In addition, generating the antigen antibody nanobeads (S120) may be to form at least one of an electric field and a magnetic field in the antibody nanobeads. In principle, the production of the antigen antibody nanobeads should be relied on the Brownian Movement of the antigen and the antibody nanobeads. However, there is a problem that a large amount of time and a large amount of samples are required to generate the antigen antibody nanobeads only on Brownian motion.

Therefore, when the electric and magnetic fields are applied to the antigen and the antibody nanobeads, the antibody nanobeads having the genome and the metal component may move more, and the number of antigen antibody nanobeads may increase with more movement. Could be. This may be a more active and efficient means for generating antigen antibodies nanobeads.

Next, at least one of an electric field and a magnetic field is formed on the generated antigen antibody nanobead to bind the generated antigen antibody nanobead and the second antibody (S130) to determine whether an antigen is present in the sample. It may be for detecting nanobeads on which the judgment is based.

According to a general method, the antigen antibody nanobead is bound to the second antibody during Brownian movement, it is detected to determine whether the antigen is present in the sample. But relying on the Brownian movement has many problems, as mentioned above.

Therefore, by applying at least one of an electric field and a magnetic field to the antigen antibody nanobead to force the antigen antibody nanobead to move closer to the second antibody, the reaction between more antigenic antibody nanobead and the second antibody Is to give this a chance to happen.

Here, at least one of the electric field and the magnetic field formed in the step (S130) of combining the generated antigenic antibody nanobead and the second antibody may be non-uniform.

When the electric field and the magnetic field are uniformly formed, since the antigen antibody nanobeads only maintain the alignment state, the antigen antibody nanobeads are formed unevenly to exert a force to move the antigen antibody nanobeads.

Force to move to the antigen antibody nanobead may be located close to the second antibody. This will increase the number of reactions, and an increase in the number of reactions will be a means to make antigen detection easier.

Here, the second antibody may be attached to a fixed position in the step of combining the generated antigen antibody nanobead and the second antibody. In order to easily detect the antigen antibody nanobead bound to the second antibody, it is necessary to know the position of the antigen antibody nanobead, so that the second antibody is attached to a fixed position so that the antigen can be detected more easily. .

In addition, the force applied by applying at least one of an electric field and a magnetic field to the antigen antibody nanobead may be controllable within a range in which the antigen antibody nanobead already attached to the second antibody does not fall.

Next, the step of detecting the antigen antibody nanobead bound to the second antibody (S140) may be to determine the presence of the antigen in the sample by detecting the antigen antibody nanobead.

There may be various methods for detecting the antigen antibody nanobeads, but in general, using fluorescent nanobeads as nanobeads, and in order to detect the antigen antibody nanobeads, The light can be irradiated to cause a fluorescent reaction.

In addition, various methods that can be detected using the features of the nanobeads may exist, and any method capable of detecting using the features of the nanobeads may be applied to the present invention.

2 is a block diagram illustrating an antigen detection apparatus using an antigen detection method according to an embodiment of the present invention.

Referring to FIG. 2, an antigen detecting apparatus 200 using an antigen detecting method according to an embodiment of the present invention binds an antibody nanobead formed of a first antibody and nanobeads with an antigen to antigen antigen nanobeads. Mixed region 210 for generating a detection region, a detection region 220 for coupling the antigen antibody nanobead and the second antibody and an electromagnetic field for forming at least one of an electric field and a magnetic field in at least one of the mixing region and the detection region It may be configured to include a generator 230.

First, the nanobeads used in the antigen detection apparatus 200 may be configured to include at least one of a dielectric and a metal. That is, the surface of the nanobead may be formed by coating at least one of a dielectric and a metal.

By coating at least one of a dielectric and a metal on the surface of the nanobead, the nanobead may be subjected to a force in the electric and magnetic fields using the dipole moment of the dielectric. Depending on the characteristics of the metal component, it will be able to receive forces in the electric and magnetic fields.

In addition, the nanobeads used in the antigen detection apparatus 200 may be configured to include a fluorescence component. That is, as the fluorescent component is included, a method of irradiating light to which the fluorescent component reacts may be used as a method for detecting an antigen in a sample.

Next, the mixed region 210 for generating the antigen antibody nanobead by combining the antibody nanobead formed with the first antibody and the nanobead with the antigen is a sample for determining whether the antigen is present and the The solution containing the antibody nanobead may be a region mixed with each other.

The antigen and the antibody nanobead in the solution will in principle be able to bind by Brownian motion. In addition, the antigen and the antibody nanobead by at least one of the electric and magnetic fields applied by the electromagnetic field generator 230 will have more reaction opportunities than when relying on the general Brownian motion.

Next, the detection region 220 for binding the antigen antibody nanobead and the second antibody may be a solution containing the antigen antibody nanobead is combined with the second antibody.

Here, the second antibody may be attached to the detection area. That is, the antigen antibody nanobead will bind to the second antibody attached to the detection region by Brownian motion.

In addition, the antigen antibody nanobead may move to a position closer to the second antibody by at least one of an electric field and a magnetic field applied by the electromagnetic field generator 230, and thus may have more opportunities for binding. will be.

Next, the electromagnetic field generating unit 230 for forming at least one of an electric field and a magnetic field in at least one of the mixing region and the detection region applies force to the antibody nanobeads in the mixing region to actively mix with the antigen. Promoting may be induced, and in the detection region, the antigen antibody nanobead may be applied to induce active binding with the second antibody.

Here, at least one of the electric field and the magnetic field of the electromagnetic field generating unit may be nonuniform. In other words, the antibody nanobeads and antigen-antibody nanobeads will be energized according to the non-uniform electric and magnetic fields, thereby providing more binding opportunities according to the movement of the antibody nanobeads and antigen-antibody nanobeads. will be.

3 is a conceptual diagram illustrating a microfluidic chip using an antigen detection method according to an embodiment of the present invention.

Referring to FIG. 3, the microfluidic chip 300 using the antigen detection method according to an embodiment of the present invention binds an antibody nanobead formed of a first antibody and nanobeads with an antigen to antigen antigen nanobeads. A mixed region 310 for generating a detection region, a detection region 320 for coupling the antigen antibody nanobead and the second antibody, and an electromagnetic field for forming at least one of an electric field and a magnetic field in at least one of the mixing region and the detection region. It may be configured to include a generator 330.

Since the mixed region 310, the detection region 320, and the electromagnetic field generator 330 have been described above, a detailed description thereof will be omitted.

In addition, the electromagnetic field generator 330 may be formed of at least one of an electrode array and a micro coil array. This is to unevenly generate at least one of an electric field and a magnetic field, and to increase the efficiency of antigen detection by controlling the electric field and the magnetic field.

In addition, the second antibody attachment region 321 is a region to which the second antibody is attached, and applies the force to the antigen antibody nanobead so as to move to the region to which the second antibody is attached. The chance of binding between the two antibodies and the antigen antibody nanobead will be increased. In other words, the efficiency of antigen detection may be improved.

Although described with reference to the above embodiments, those skilled in the art will understand that various modifications and changes can be made without departing from the spirit and scope of the invention as set forth in the claims below. Could be.

200: antigen detection device 210: mixing region
220: detection area 230: electromagnetic field generating unit
300: microfluidic chip 310: mixing region
320: detection region 321: second antibody attachment region
330: electromagnetic field generating unit

Claims (20)

Combining the first antibody with nanobeads to produce antibody nanobeads;
Combining the generated antibody nanobeads with an antigen to generate antigen antibody nanobeads;
Combining at least one of an electric field and a magnetic field with the generated antigenic antibody nanobead and the second antibody; And
The antigen detection method comprising the step of detecting the antigen antibody nanobead bound to the second antibody. The method of claim 1,
The nanobead comprises at least one of a dielectric (dielectric) and a metal (metal) comprises an antigen detection method. The method of claim 2,
The genome has a dipole moment (dipole moment), characterized in that the antigen detection method. The method of claim 1,
The nanobead comprises an fluorescence component (fluorescence) comprising an antigen detection method. The method of claim 1,
Generating the antigen antibody nanobeads, characterized in that to form at least one of an electric field and a magnetic field in the antibody nanobeads. The method of claim 1,
At least one of the electric and magnetic fields to form in the step of combining the generated antigen antibody nanobead and the second antibody is characterized in that the non-uniform. The method of claim 1,
The second antibody is attached to a fixed position in the step of binding the generated antigen antibody nanobead and the second antibody, characterized in that the antigen detection method. A mixed region for binding antigen nanobeads formed of the first antibody and nanobeads with the antigen to generate antigen antibody nanobeads;
A detection region for binding the antigen antibody nanobead and the second antibody; And
And an electromagnetic field generator for forming at least one of an electric field and a magnetic field in at least one of the mixed region and the detection region. The method of claim 8,
The nanobead comprises at least one of a dielectric (dielectric) and metal (metal) antigen detection device, characterized in that configured. 10. The method of claim 9,
The genome has a dipole moment (dipole moment), characterized in that the antigen detection device. The method of claim 8,
The nanobead comprises an fluorescence component (fluorescence) comprising an antigen detection device. The method of claim 8,
At least one of an electric field and a magnetic field of the electromagnetic field generating unit is nonuniform. The method of claim 8,
The second antibody is attached to the detection region, characterized in that the antigen detection device. A mixed region for binding antigen nanobeads formed of the first antibody and nanobeads with the antigen to generate antigen antibody nanobeads;
A detection region for binding the antigen antibody nanobead and the second antibody; And
And an electromagnetic field generator for forming at least one of an electric field and a magnetic field in at least one of the mixing region and the detection region. The method of claim 14,
And the nanobead comprises at least one of a dielectric and a metal. 16. The method of claim 15,
Wherein said dielectric has a dipole moment. The method of claim 14,
The nano-bead is a microfluidic chip comprising a fluorescence component. The method of claim 14,
The second antibody is attached to the detection region, the microfluidic chip. The method of claim 14,
At least one of an electric field and a magnetic field of the electromagnetic field generating unit is nonuniform. The method of claim 14,
The electromagnetic field generator is formed of at least one of an electrode array (array) and a micro coil array (micro coil array).
KR1020100083629A 2009-10-09 2010-08-27 Method of detecting antigen, apparatus and micro fluidic chip for detecting antigen using the same KR20110039181A (en)

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JP2006247535A (en) * 2005-03-10 2006-09-21 Toyo Univ Method for promoting reaction by using rotating magnetic field

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