KR20110038201A - Microfluidic devices and a method of preparing the same - Google Patents

Abstract

PURPOSE: A method for manufacturing a microfluidic channel which is modified into hydrophilicity is provided to be applied to medical equipment. CONSTITUTION: A method for manufacturing a microfluidic channel of which surface is hydrophilic comprises: a step of forming a channel substrate using polymeric materials; and a step of laminating anionic polymeric electrolyte layer and cationic polymeric electrolyte on the channel substrate. A microfluidic device or biosensor contains the microfluidic channel.

Description

Microfluidic device and its manufacturing method {MICROFLUIDIC DEVICES AND A METHOD OF PREPARING THE SAME}

The present invention relates to a microfluidic channel in which the surface in the micro rapeseed channel is hydrophilically modified, a method for producing the same, and a method for hydrophilically modifying the surface in the micro rapeseed channel.

A bio chip is a chip in which DNA, protein, etc. molecules probes to be analyzed are attached to a substrate at a high density, and gene expression is detected by detecting hybridization between the probes and a target material in a sample. Analyze gene defects, protein distribution, response patterns, and more. Biochips can be divided into microarray chips attached to a solid substrate and microfluidic chips attached to microfluidic channels according to the attachment form of the probe.

Microfluidic chips are called lab-a-chips that automatically process the entire process of an experiment, such as sample injection, hybridization and detection, into a single chip. It is made of various materials such as quartz, plastic, or silicon, and a plurality of fine channels narrower than the hairs are staggered inside the chip. Many complex experiments can be performed at once by flowing fluid through these microchannels.

Various driving principles of the microfluidic technology related to the flow generation to control and control the microfluidic fluid include the microactuating method, the fluid injection method in which the micromachined micropump and valve are implemented on the flow path or the chamber. Pressure-driven method to apply pressure to the part, electrophoretic method or electroosmotic method to transfer fluid by applying voltage between microchannels, and capillary flow method using capillary force (Capillary flow method) is representative.

Microfluidic devices using this driving principle can be classified into active microfluidic components and passive microfluidic components. Active devices use micro-pumps and valves driven by electric and mechanical external forces to drive fluid into micro rapeseed channels. Passive devices use natural forces and change the surface or shape of flow paths or chambers. Fluid drive into the microfluidic channel is realized.

Among them, the microfluidic device using the capillary flow is a passive device, and stops, transfers, or further moves the fluid by using a principle in which attraction or repulsion naturally occurs due to the surface tension between the inner surface of the microfluidic channel and the fluid. Allow speed control. In very small fluid systems, the ratio of surface area to volume increases, so that the force associated with the surface plays a relatively important role, especially when the liquid interface is exposed to gas, and when it encounters a solid wall. At an angle, capillary flow occurs when applied to the capillary.

The capillary flow method uses a capillary phenomenon that occurs naturally in the microchannel, so that a small amount of fluid placed in the fluid injection portion moves naturally and immediately along a given microfluidic channel without any additional device. The fluid control device using the capillary flow Since there is no driving device, there is no need for an additional power supply, so that the mother body including the biochip can be miniaturized, manufacturing costs and operating costs are reduced, and there are almost no failures. Therefore, the research on the design of the microfluidic system using this is being actively conducted.

On the other hand, unlike semiconductor fabrication for the purpose of fluid devices, technology for controlling the properties of the surface rather than the structure plays a more important role as it must perform a reaction or a sensing function by flowing a fluid on the surface of the device. Among microfluidic devices, most biodevices used for biological, biochemical, and medical applications use water as a medium. Therefore, organic materials based on polymers are widely used as non-corrosive and light materials. In addition, some of the polymer materials (eg, PDMS, PMMA, PC, PS, COC, etc.) are typically used because they need to ensure transparency for optical sensing.

However, all of the microfluidic channels using these polymers have strong surface hydrophobicity, and thus, samples containing water are used. Most of the biomaterials have nonspecific adsorption due to hydrophobic surfaces and hydrophobic bonds and van der Waals bonds. The hydrophobic surface causes a low wettability of the aqueous solution, and because the contact angle is too high, it is impossible to inject the sample by the capillary force or to move it along the channel. There is a problem that must be mounted or surface treatment.

It is easier to apply permanent surface treatment technology in many ways than to improve the complexity, limitations, and economics of device configuration when mounting a pump. However, in general, surface treatment techniques that are generally used, such as plasma treatment only guarantees a short-term effect, or chemical treatment of the element material is a problem in most cases.

When the microfluidic channel is formed, the surface of the polymer materials used to form the flow path substrate is generally hydrophobic. Therefore, when the hydrophilic sample including water is injected, the contact angle is too high to inject the sample by capillary force. There was a problem that the movement along the channel is impossible.

The present invention, in order to solve the problems of the prior art as described above, the microfluidic surface to modify the surface of the flow path substrate using a polymer material with a polymer electrolyte coating film so that a very small amount of fluid flow by the natural capillary flow by the capillary force A method of manufacturing a channel, a method of surface modifying a microfluidic channel using the polymer electrolyte layer coating film, and a surface modified microfluidic channel.

In order to achieve the above object, the present invention provides a method for producing a microfluidic channel whose surface is hydrophilic. More specifically, forming a flow path substrate using a polymer material; And it provides a method of producing a hydrophilic microfluidic channel of the surface comprising the step of alternately stacking an anionic polymer electrolyte layer and a cationic polymer electrolyte layer on the flow path substrate.

The present invention provides a method for surface modification of a microfluidic channel using a polymer electrolyte coating film. More specifically, forming a flow path substrate using a polymer material; Coating the flow path substrate with a polycation polymer solution to form a polycation thin film on the substrate; And forming a polyanion thin film on the substrate by coating the flow path substrate on a polyanion polymer solution.

Hereinafter, the present invention will be described in more detail.

Since the surface of the polymer materials used to form the flow path substrate is generally hydrophobic when the existing microfluidic channel is formed, the samples used in the experiments of biological, biochemical and medical applications exhibit water hydrophilicity using water as a medium. There was a problem that the contact angle was too high when passing through the microfluidic channel using a polymer material, so that the injection of the sample by the capillary force or the movement along the channel was impossible. ) Or surface treatment. In many respects, it was easier to apply permanent surface treatment technology to improve the hydrophilicity of polymer materials, compared to the problems of complexity, limitations, and economics of device configuration when installing a pump. In general, surface treatment technologies used in the short term, such as plasma treatment, guarantee a short-term effect, or chemical durability of the device material is often a problem, and conventional surface treatment techniques require a long process or expensive equipment. The economic burden was great, and the conventional dry surface treatment method had a problem that the environmental pollution due to the by-products are likely.

In an effort to solve the above problems, the present inventors can maximize the hydrophilicity of the surface of the microfluidic channel when the coating film is prepared by alternately stacking the anionic polymer electrolyte layer and the cationic polymer electrolyte layer, It can increase the stability by adjusting the thickness and functional group of coating film according to the number of times. This lamination method does not need expensive equipment, and it is a water-based eco-friendly process to meet the purpose of the product. The present invention was completed based on this, confirming that the microreactor function using the microchannel device could also be performed by applying chemical active groups to the laminated coating film.

In the present specification, the 'polymer', 'polymer' and 'polymer' may be used in the same sense, and may be used interchangeably with each other without a difference in meaning unless otherwise specified.

In the present specification, 'microfluidic channel' means a flow path through which a micro unit of fluid can pass in order to use a microfluid, and includes at least one microfluid inlet, a storage region connected to each inlet, It may be provided with a plurality of outlets connected to each storage area and a fine channel circuit connecting the respective inlet and outlet. The microchannel flow path of the present specification is a concept including a set of microchannels formed by connecting a plurality of microchannels. More specifically, the microchannel flow path is formed by interconnecting at least one branching region, and each branching region may include an inflow channel into which fluid is introduced and two or more branching channels branched from the inflow channel. That is, the fluid passing through the branching region has a characteristic of branching to each branching channel in inverse proportion to the resistance in the branching channel, and the branching channel of one branching region may be connected to the inlet channel of the other branching region and finally connected to the outlet port. .

In the present specification, the "flow path board" refers to a substrate in which recesses, specifically, holes and grooves are formed to form fluid reservoirs and fluid passages connecting the flow paths at regular intervals. Fluid outlets may be formed.

In the present specification, the expression of the pH of the polymer electrolyte can be expressed as follows. For example, when the coated polycation polymer is PAH and the polyanion polymer is PAA and their pH is pH 4.5 and pH3.0, respectively, 'PAH / PAA (4.5 / 3.0)' or 'PAH (4.5) / PAA (3.0) '.

The present invention may be a method for producing a microfluidic channel whose surface is hydrophilic.

More specifically, the present invention, forming a microfluidic channel using a polymer material; And an anionic polymer electrolyte layer and a cationic polymer electrolyte layer are alternately stacked on the micro rapeseed channel.

In addition, the present invention comprises the steps of etching the recess for forming a micro rapeseed channel through which microfluidic fluid can flow; Alternately stacking an anionic polymer electrolyte layer and a cationic polymer electrolyte layer on the recessed substrate; The method may be a method of manufacturing a hydrophilic microfluidic channel including a step of manufacturing a micro rapeseed channel by combining the substrate on which the anionic polymer electrolyte layer and the cationic polymer electrolyte layer are laminated.

In the alternately stacking of the anionic polymer electrolyte layer and the cationic polymer electrolyte layer, the lowermost layer may be stacked such that the cationic polymer electrolyte layer and the uppermost layer are the anionic polymer electrolyte layer. In addition, the step of alternately stacking the anionic polymer electrolyte layer and the cationic polymer electrolyte layer may be an anionic polymer electrolyte layer and a cationic polymer electrolyte layer may be 1 to 15 layers or 1 to 12 layers, respectively, preferably May be 5 to 12 layers or 5 to 10 layers.

In the present invention, in relation to the description of the alternating stacking structure, a multilayered film in which one layer of each of two polymer electrolyte layers of different types is stacked is called 1bilayer, and the alternating stacking structure is 1bilayer to 15bilayer or 1bilayer to 12bilayer in this respect. And preferably 5 bilayer to 12 bilayer or 5 bilayer to 10 bilayer. When the alternating stacking structure is laminated too little, the improvement of hydrophilicity does not exhibit the effect of the present invention. When the alternating stacking structure is stacked too much, the alternating stacking structure, that is, the polymer layer may be unstable, and thus the scope of the present invention is limited. desirable.

The anionic polymer electrolyte layer may be preferably made of any one selected from the group consisting of hyaluronic acid (HA) and polyacrylic acid (PAC), and the cationic polymer electrolyte layer is preferably It may be made of any one selected from the group consisting of poly allylamine hydrochloride (poly (allylamine hydrochloride), PAH) and poly acrylamide (PAAm).

In addition, the anionic polymer electrolyte may be adjusted to a pH of 4.3 to 4.7, the cationic polymer electrolyte layer may be adjusted to a pH of 2.8 to 3.2. When the pH of the polymer electrolyte layer is too high or low, the hydrophilic enhancement effect by lamination, that is, the wettability synergistic effect is limited, the scope of the present invention is preferred.

The flow path substrate is made of polyurethane (PU), polysulfone (PSU), liquid crystal polymer (LCP), polyetherimide (PEI), polyactide (polylactide) , Polymethyl methacrylate (PMMA), polycarbonate (PC), cycloolefin copolymer (COC), polydimethylsiloxane (PDMS), polyamide (PA), polyethylene (polyethylene; PE), polypropylene (PP), polyphenylene ether (PPE), polystyrene (PS), polyoxymethylene (POM), polyetheretherketone (PEEK) , Polytetrafluoroethylene (PTFE), polyvinylchloride (PVC), polyvinylidene fluoride (PVDF), polybutylenetetraphthalate (polybuty leneterephthalate (PBT), fluorinated ethylenepropylene (FEP), perfluoralkoxyalkane (PFA), and may be prepared from any one selected from the group consisting of, and is currently industrially applied, and polymer stability This may be an excellent polymethylmethacrylate or cycloolefin copolymer.

In addition, the present invention may be a method of modifying the surface of the microfluidic channel to a hydrophilic surface comprising the step of alternately stacking the anionic polymer electrolyte layer and the cationic polymer electrolyte layer in the microfluidic channel.

In the stacking of the anionic polymer electrolyte layer and the cationic polymer electrolyte layer, the anionic polymer electrolyte and the cationic polymer electrolyte may be alternately injected into the micro rapeseed channel using a pump. Even in the case of the polymer electrolyte layer, since it is not easy to be injected into the micro rapeseed channel before the surface is modified, it is preferable to inject using a pump in terms of lamination efficiency.

The step of alternately stacking the anionic polymer electrolyte layer and the cationic polymer electrolyte layer may be laminated so that the lowermost layer is a cationic polymer electrolyte layer, the uppermost layer is an anionic polymer electrolyte layer, the anionic polymer electrolyte has a pH It is adjusted to 4.3 to 4.7, the cationic polymer electrolyte layer may be adjusted to pH of 2.8 to 3.2.

In addition, the present invention may be a microfluidic channel having a hydrophilic surface that includes a laminated structure of an anionic polymer electrolyte layer and a cationic polymer electrolyte layer on the surface of the micro rapeseed channel.

The micro rapeseed channel is preferably made of polymethyl methacrylate (PMMA) or cycloolefin copolymer (COC), and the anionic polymer electrolyte layer is preferably hyaluronic acid and polyacrylic acid. It is made of any one selected from the group consisting of, the cationic polymer electrolyte layer may be preferably made of any one selected from the group consisting of poly allylamine hydrochloride and poly acrylamide.

The micro rapeseed channel can be applied to various experimental or diagnostic devices including microfluidic devices, biochips or biosensing devices.

In this aspect, the present invention may be a microfluidic device characterized in that it comprises the micro rapeseed channel or a biosensing device comprising the micro rapeseed channel.

When the polymer electrolyte multilayer film of the present invention is coated, the hydrophobicity of the surface of the flow path substrate of the micro rapeseed channel is modified, so that excellent contact with the hydrophilic sample can be ensured, and the micro rapeseed channel is not required without a separate means such as a pump. Since the hydrophilic sample can be easily introduced, the microfluidic channel or the microfluidic device or the biosensing device including the same can be miniaturized and reduced in weight. Production may be possible.

Hereinafter, preferred examples and comparative examples of the present invention will be described in order to explain the present invention in detail. However, the following examples are only for illustrating the present invention and the scope of the present invention is not limited in any way by the following examples, and modifications which are commonly performed in the art are included in the scope of the present invention. .

[ Example  And Experimental Example ]

Preparation Example 1 : Polymer electrolyte Multilayer  Produce

All polymer aqueous solution and washing solution needed to form a multilayer film were prepared using deionized water (> 18 M,) produced by Millipore's Milli-Q water purifier. The aqueous solution of polyacrylamide (PAAm) was 0.01M and the aqueous solution of hyaluronic acid (HA) was made 0.002M (based on the repeat unit molecular weight), filtered through a 0.45 pore filter to remove dust and microorganisms, and 0.1M HCl The aqueous solution of poly (acrylic acid) (PAH) was adjusted to pH 4.5 and the solution of poly (allylamine hydrochloride) (PAA) to pH 3.0.

Prior to laminating the polymer multilayer film, the substrate to be coated with a coating film (PMMA or COC.) Was washed with an ultrasonic cleaning with a thin micro detergent solution and dried with nitrogen gas. The dried substrate was confined to the PAH solution inside the channel for 15 minutes using a pump (Cole Parmer, Masterflex Model 7520-45) and washed twice with deionized water for 2 minutes or more to form an initial coating film. Confined for a minute and washed in the same manner as above. After lamination of the polymer multilayer film was dried with nitrogen gas and stored until the next step. Plasma Cleaner PDC-32G (HARRICK PLASMA) was used when plasma treatment was required in the pretreatment of the experiment.

Meanwhile, in order to identify the polymer electrolyte multilayer film in the channel, Methlyene blue was injected into the channel, maintained for 15 minutes, and washed with deionized water twice for 2 minutes. The formation of the polymer electrolyte multilayer film in the channel was determined by the staining of Methlyene blue. 1 shows the results of confirming the formation of the prepared multilayer using the dyeing of the Methlyene blue.

Experimental Example 1 Polymer Electrolyte Multilayer Film Lamination on COC Substrate

Example 1 -1 wettability (wetting, Wettability ) Measurement

Wetting test was performed on the multilayer film (6BL) having an acid group as the outermost film and the multilayer film (6.5BL) having an amine group among the multilayer film laminated on the COC substrate using a CA-A Contact angle analyzer of Face Company. Contact angles were measured using PAH / PAA (4.5 / 3.0) and PAH / HA (10.0 / 10.0) films. The water used for measuring the contact angle was used after filtering the purified water and forming a drop of 15 ul by using a syringe, and slowly raised the measuring plate on which the film was placed so as to contact the water droplets, and the results are shown in FIG. 2.

As shown in FIG. 2, the two types of polymer electrolyte multilayers showed good wettability, compared to the bare case without any treatment, and among them, the multilayer film having an acid group on the outermost film was 6BL. The lowest contact angles showed good wettability in both PAH / PAA (4.5 / 3.0) and PAH / HA (10.0 / 10.0) films, especially when the pH of the polycationic polymer was 4.5 and the pH of the polyanionic polymer was 3.0 , PAH / PAA (4.5 / 3.0) showed the best wettability.

Example 1 -2 Polymer electrolyte Multilayer  Measurement of flow in channels before and after lamination

The experiment was performed in the same manner as in Example 1-1, and the results of the experiment by introducing the wettability of the coating layer into the COC channel are shown in FIG. 3.

As shown in FIG. 3, when the PAH (4.5) / PAA (3.0) 6BL was coated before and after the multilayer coating, the contact angle, which was 110 degrees before the coating, was lowered to 30 degrees after the coating. Before the coating film was formed, no flow occurred without external pressure due to the hydrophobic surface. The above results measured the contact angle when water was pushed in using a pump. On the other hand, when the polymer electrolyte multilayer film was introduced, the wettability of the surface was increased. As a result, capillary force was generated and the flow was generated without external pressure. Thus, it was confirmed that the polymer electrolyte multilayer film could control the flow inside the channel.

Example 1 Within -3 channels Polymer electrolyte Multilayer film  Measurement of Cell Adsorption on Films

In order to examine the interaction between PAH / PAA polyelectrolyte multilayer membrane film and cells, PAH (4.5) / PAA (3.0) 6BL multilayer films were prepared using HEK 293 kidney cells and raw cells. The cells were injected into the stacked channels, and the results of the observation immediately after the injection of the cells and after 24 hours were as shown in FIG. 4.

As shown in FIG. 4, 24 hours after the injection of the cells, the cells tended to aggregate, and the behavior of the cells was found to be non-toxic without significant difference in bare and coated channels. .

Preparation Example 2 : PMMA  Substrate Polymer electrolyte Multilayer  Produce

All polymer aqueous solution and washing solution needed to form a multilayer membrane were prepared using deionized water (> 18 M) produced by Millipore's Milli-Q water purifier. PAH and PAA aqueous solutions were made into 0.01M and filtered through a filter with 0.45 pores to remove dust and microorganisms.The pH of the polymer electrolyte solution laminated with 0.1M HCl or NaOH solution was 8.5 and PAA, respectively. pH was adjusted to 3.0.

On the other hand, when manufacturing a polymer multilayer membrane with a PMMA substrate, the substrate to be coated (PMMA) is washed with a thin detergent solution, washed with deionized water several times and dried with nitrogen gas, using a Plasma cleaner PDC-32G from Hirrick Plasma Atmospheric Plasma treatment was performed for 2 minutes. First, soak 15 minutes (20 minutes for the first time) in PAH solution and wash it with deionized water for 2 minutes or more twice, then put 15 minutes in PAA solution and wash it for 2 minutes or more with deionized water again 1bilayer to form a coating film, and the process was repeated until the desired thickness.

Experimental Example  2 : PMMA  On a substrate Polymer electrolyte On multilayer  Substrate change measurement

Example 2 With -1 substrate changes Of the effect of wettability  Measure

The experiment was performed in the same manner as in Example 1-1, except that PMMA was used as a substrate instead of COC, and PAH / PAA (4.5 / 3.0) and PAH / HA (4.5 / 3.0) films were used. 5 is shown.

As shown in FIG. 5, compared with the case of bare barely treated, the polymer membranes to be electrolyzed in both the PAH / PAA (4.5 / 3.0) and PAH / HA (4.5 / 3.0) films are improved in wettability. The results were shown. This shows the same tendency as compared with the COC substrate shown in Example 1-1, which means that the polymer electrolyte coating film is not significantly affected by the type of substrate and its wettability is improved. In addition, when HA of pH 3.0 was used, the wettability was improved at a low contact angle, and it was confirmed that the wettability was good when the pH of the polycationic polymer was 4.5 and the pH of the polyanionic polymer was 3.0.

Example 2 -2 In a multilayer  According to film thickness Contact angle  Measure

The experiment was carried out in the same manner as in Example 2-1, except that the film thickness was 6BL, 7BL, 8BL, 9BL, and 10BL rolls in the PAH / PAA (4.5 / 3.0) multilayer to affect the contact angle. It was confirmed that the effect, the results are shown in FIG.

As shown in FIG. 6, the lowest contact angle was measured at the time of stacking 6BL. Although the contact angle increased as the thickness of the multilayer increased, the width of the multilayer film was about 10 degrees. The contact angle is a measurement value that is highly influenced by the surrounding environment, and it is a measurement value that can be changed by weather, humidity, etc., but it was not a big difference of more than 10 degrees, but 6BL was confirmed to be the best. Therefore, it can be seen that the effect of the 6BL multilayer film is sufficient, and the increase in the thickness of the polymer electrolyte multilayer film does not change the contact angle significantly.

Example 2 -3 Polymer electrolyte Multilayer film  stability

In the same manner as in Preparation Example 1 and Example 2-1, PMMA substrate surface-modified with plasma and PMMA substrate surface-modified with 6BL film thickness in PAH / PAA (4.5 / 3.0) multilayer film were prepared immediately after treatment. 1, 2, 3, 4, 5, 6, 7, 8, and 9 by checking the change of contact angle over time, the stability of the polymer electrolyte multilayer film was measured, and The results are shown in FIG.

As shown in FIG. 7, in the case of the plasma method capable of sufficiently modifying the surface to be hydrophilic, the rate of increase in the case of surface modification using a polymer electrolyte multilayer membrane is increased to 57 degrees from 1 day when left in air and changed to hydrophobicity. It was found to be less than 35 degrees even after one week and still exhibit hydrophilicity, indicating that stability is greater than that of the surface modification method using the conventional plasma treatment.

Experimental Example 2 -4 Non-porous In a multilayer  Determination of Wetting (Wetability) According to Film Thickness

A polymer multilayer film was prepared from PMMA substrate according to Preparation Example 2, wherein the film layers were 1BL, 1.5BL, 3BL, 3.5BL, 5BL and 5.5BL, and the contact angle of water was measured using a CA-A contact angle analyzer of Face Company. Measured. The water used for measuring the contact angle was used after filtering the purified water and forming a droplet of 15 ul using a syringe, and slowly raising the measuring plate on which the film was placed so as to contact the droplets.

As shown in FIG. 8, the thicker the multilayer thin film was, the lower the contact angle was. When the final dipping solution formed PAA, the wettability was improved.

1 is a photograph confirming the result of stacking a polymer electrolyte layer inside a COC channel using methylene blue according to an embodiment of the present invention.

Figure 2 is a graph confirming the change in contact angle according to the pH of the laminated polymer electrolyte according to an embodiment of the present invention, Figure 2a is a graph showing a case where the pH is adjusted to pH4.5 / pH 3.0, Figure 2b Is a graph showing the case where the pH is adjusted to pH10.0 / pH10.0.

Figure 3 is a result of confirming the fluid flow and contact angle in the channel before / after the stacking of the polymer electrolyte multilayer film according to an embodiment of the present invention, Figure 3a is a photo before lamination, Figure 3b is a photo after lamination.

Figure 4 is a photograph showing the results of the experiment confirming the adhesion of cells in the front and rear channels of the polymer electrolyte multilayer membrane according to an embodiment of the present invention.

5 is a result of measuring the wettability by measuring the contact angle of water on the polymer multilayer film, Figure 5a is a graph showing the lamination results of PAH / PAA, Figure 5b is a graph showing the lamination results of PAH / HA.

6 is a graph confirming the contact angle test results according to the film thickness in the PAH / PAA multilayer film according to the embodiment of the present invention according to the contact angle thickness.

FIG. 7 is a graph confirming change in contact angle after treatment in order to confirm the persistence of the surface modification effect of the plasma method and the multilayer coating method of the present invention for surface modification of a PMMA fluid substrate, according to an embodiment of the present invention. Figure 7a is a graph showing the multilayer coating method of the present invention, Figure 7b is a graph showing the plasma treatment method.

8 is a graph confirming the contact angle test results according to the film thickness of the multilayer film in the PMMA fluid substrate according to the embodiment of the present invention according to the thickness of the contact angle.

Claims (14)

Forming a flow path substrate using a polymer material; And Alternately stacking an anionic polymer electrolyte layer and a cationic polymer electrolyte layer on the flow path substrate Method for producing a microfluidic channel is hydrophilic surface comprising a. Manufacturing a flow path substrate by etching a recess to form a flow path through which a microfluid can flow; Alternately stacking an anionic polymer electrolyte layer and a cationic polymer electrolyte layer on the flow path substrate; Preparing a micro rapeseed channel by combining a flow path substrate on which the anionic polymer electrolyte layer and the cationic polymer electrolyte layer are stacked; Method for producing a microfluidic channel is hydrophilic surface comprising a. The method according to claim 1 or 2, The step of alternately stacking the anionic polymer electrolyte layer and the cationic polymer electrolyte layer is characterized in that the lowest layer is a cationic polymer electrolyte layer, the uppermost layer is laminated so that the anionic polymer electrolyte layer A method for producing a microfluidic channel whose surface is hydrophilic. The method according to claim 1 or 2, The step of alternately stacking the anionic polymer electrolyte layer and the cationic polymer electrolyte layer is characterized in that the anionic polymer electrolyte layer and the cationic polymer electrolyte layer is 5 to 12 layers, respectively A method for producing a microfluidic channel whose surface is hydrophilic. The method according to claim 1 or 2, The flow path substrate is made of polyurethane (PU), polysulfone (PSU), liquid crystal polymer (LCP), polyetherimide (PEI), polyactide (polylactide) , Polymethyl methacrylate (PMMA), polycarbonate (PC), cycloolefin copolymer (COC), polydimethylsiloxane (PDMS), polyamide (PA), polyethylene (polyethylene; PE), polypropylene (PP), polyphenylene ether (PPE), polystyrene (PS), polyoxymethylene (POM), polyetheretherketone (PEEK) , Polytetrafluoroethylene (PTFE), polyvinylchloride (PVC), polyvinylidene fluoride (PVDF), polybutylenetetraphthalate (polybuty leneterephthalate (PBT), fluorinated ethylene propylene (FEP), and perfluoralkoxyalkane (perfluoralkoxyalkane; PFA) A method for producing a microfluidic channel whose surface is hydrophilic. The method according to claim 1 or 2, The anionic polymer electrolyte is pH is adjusted to 4.3 to 4.7, the cationic polymer electrolyte layer is characterized in that the pH is adjusted to 2.8 to 3.2 A method for producing a microfluidic channel whose surface is hydrophilic. Alternately stacking an anionic polymer electrolyte layer and a cationic polymer electrolyte layer on the microfluidic channel Method for modifying the microfluidic channel surface comprising a hydrophilic surface. The method of claim 7, wherein In the stacking of the anionic polymer electrolyte layer and the cationic polymer electrolyte layer, the anionic polymer electrolyte and the cationic polymer electrolyte are alternately injected into the micro rapeseed channel using a pump. A method of modifying the surface of a microfluidic channel to a hydrophilic surface. The method of claim 7, wherein The step of alternately stacking the anionic polymer electrolyte layer and the cationic polymer electrolyte layer is characterized in that the lowest layer is a cationic polymer electrolyte layer, the uppermost layer is laminated so that the anionic polymer electrolyte layer A method of modifying the surface of a microfluidic channel to a hydrophilic surface. The method of claim 8, The anionic polymer electrolyte is pH is adjusted to 4.3 to 4.7, the cationic polymer electrolyte layer is characterized in that the pH is adjusted to 2.8 to 3.2 A method of modifying the surface of a microfluidic channel to a hydrophilic surface. A microfluidic channel having a hydrophilic surface having an alternating structure of an anionic polymer electrolyte layer and a cationic polymer electrolyte layer on a surface of a flow path substrate. The method of claim 11, wherein the flow path substrate is made of polymethyl methacrylate (PMMA) or cycloolefin copolymer (CCO), and the anionic polymer electrolyte layer is made of hyaluronic acid and polyacrylic acid. It is made of any one selected from the group consisting of, wherein the cationic polymer electrolyte layer is a hydrophilic microfluidic channel of any one selected from the group consisting of poly allylamine hydrochloride and poly acrylamide. A microfluidic device comprising the microoil channel of claim 11. A biosensing device comprising the micro rapeseed channel of claim 11.

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