KR20110026271A - Method for rapidly enhancing maysin content of centipede grass - Google Patents

Abstract

PURPOSE: A method for quickly enhancing maysin content of sentipede grass is provided to enhance productivity of maysin and maysin derivative and to be used in manufacturing insecticide. CONSTITUTION: A method for enhancing maysin and maysin conten comprise a step of treating stress condition to matured leaves of centipede grass. The matured leaves are green. The maysin derivative is isoorientin or rhamnosylisoorientin. The stress condition is wounding, radiation irradiation, UV-B irradiation, or treatment at low temperature. The wounding is performed at 2/3 spot of the maturing green leaves. The radiation is gamma ray, electronic beam, or X-ray. The UV-B is irradiated every 5 minutes a day for 18-22 days. The low temperature treatment is performed at 1-10°C.

Description

Method for rapidly enhancing Maysin content of centipede grass}

The present invention is directed to a method of rapidly enhancing the maysin content of sentigrass.

Centipede grass is a slow-growing, turbulent grass that is a perennial grass that crawls and forms turf. It belongs to the family Poaceae, and its scientific name is Eremochloa ophiuroides (Munro) Hack. It is mainly distributed in China, East Asia, Indochina, the northeastern United States, Meso America, and the Caribbean, and is widely adapted to various soil conditions, but is especially resistant to moist, low acidic soils and low-quality sandy soils. As a morphological feature, the leaves are 15-30 mm long, 2-4 mm wide, flat with a mid vein, hairless except for the collar part, and the leaf ends are round. The stem has a very firm sheath, and the roots are thin branched stolons. The flower part has one raceme of about 3 to 5 inches, which has a purple, slightly flat, small spikelet arranged in two rows. Have Its main use is as a lawn or turf grass and as a forage due to its high leafiness and good taste.

Maysin (maysin) is a yellow plant pigment flavonoid family found in corn, teosinte and centifedgrass is known for its antioxidant and anticancer activity. That is, when the maisin extracted from corn was injected into five human cancer cells such as colon cancer and lung cancer, the proliferation of cancer cells was inhibited and the active coral removal effect harmful to the human body was also confirmed. Thus, the production technology to increase the amount of Meisin more than 40 times in the corn beard was developed, and a new breed with a high content itself was also developed.

Accordingly, the present inventors have developed stresses such as breaking or irradiating the centipedes in order to develop a technique for rapidly producing macine from centipedes, which can withstand various soil conditions, especially wet, low soils and low fertility soils. As a result, the present invention was completed by confirming that it exhibits a similar degree of macine content and insecticidal effect as that of natural red leaves.

It is an object of the present invention to provide a method for rapidly enhancing the content of the maysine and maysine derivatives of mature centipedegrass in its natural state.

In order to achieve the above object, the present invention provides a method for rapidly enhancing the content of the Meissin and Meissin derivatives comprising the step of treating the stress conditions to mature leaves of Sentipedgrass in the natural state.

Since the productivity of the macine and the macine derivative having the insecticidal effect by the method of the present invention increases, it can be usefully used for the preparation of the pesticide.

Hereinafter, the present invention will be described in detail.

The present invention provides a method for rapidly enhancing the content of Meissin and Meissin derivatives, comprising the step of treating stress conditions on mature leaves of Sentipedgrass in its natural state.

In a specific embodiment of the present invention, in order to induce red leaves from mature greens in the natural state of centipedes grass, breaking at two-thirds of the mature green leaves, gamma rays or electron beams within a total absorbed dose of 10 Gy to 2 kGy By treating stress such as irradiation, ultraviolet irradiation and low temperature treatment, it was confirmed that the green leaves were induced to the red leaves (see FIG. 1). In the natural state, it took about 60 days to change from green to red leaves, whereas the stress treatment took about 20 days to induce red leaves. In addition, the stress-induced red leaves showed slightly lower amounts of maysin and maysin derivatives (isoorientin and rhamnosylisoorientin) than natural red leaves, but 85% The above content was shown, and the content of Meicin and Meicin derivative was higher than that of mature green leaves (see FIG. 2). Survival rates of chestnut larvae fed red leaves induced by the stress were higher than those fed mature green leaves and were similar to those fed natural red leaves (see FIG. 3).

The stress condition is bending, irradiation, ultraviolet irradiation or low temperature treatment.

The mature leaf is a green leaf, the mayine derivative is isoorientin (isoorientin) or rhamnosylisoorientin (rhamnosylisoorientin) means, but is not limited thereto.

The bending may be performed at two thirds of the mature green leaves, but is not limited thereto.

The radiation gamma rays, electron beams or X-rays can all be used, and gamma rays or electron beams are preferably used. The gamma rays are preferably irradiated with gamma rays emitted from radioactive isotopes such as cobalt (Co) -60, krypton (Kr) -85, strontium (Sr) -90, or cesium (Cs) -137. More preferably, but not limited to, emission from Co) -60 radioisotopes. The irradiation dose is preferably 10 gy-2 kGy, more preferably 50 gy-1 kGy, most preferably 100 gy-0.5 kGy. The range of the radiation dose is less than the extent to inhibit the growth of the centifed glass to die, and the leaf color of the senticapgrass is changed to show the content or the insecticidal effect of the enhanced Meicin and Meisin derivatives Set.

The ultraviolet light is composed of three types of UV-C, UV-B, and UV-A, but in the present invention, ultraviolet light is irradiated using a UV-B lamp. It is preferable to irradiate the ultraviolet rays for 5 minutes daily for 18 to 22 days, more preferably 20 days. The amount of ultraviolet irradiation is less than the extent to inhibit the growth of the centipedes grass to die, and the leaf color of the centipedes grass is set on the basis that can change the content or insecticidal effect of the enhanced Meicin and Meisin derivatives It was.

The low temperature treatment is preferably performed at 1 to 10 ℃, most preferably at 4 ℃. The range of the low temperature treatment temperature is less than the degree of inhibiting the growth of the centipedes grass to die, and the leaf color of the centipedes grass can be changed to show the content or insecticidal effect of the enhanced Meicin and Meisin derivatives Set to.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

< Example  1> Sentedgrass  Treatment Methods for Increasing Meisin and Meisin Derivative Content

In the natural state of Centipedgrass, red leaves have the highest amount of macine among mature green, yellow and red leaves. To induce red leaves, break at two-thirds of the mature green leaves, irradiate gamma or electron beams with UV-B lamps for 5 minutes (5.5 W) within the total absorbed dose of 10 Gy to 2 kGy. m ^-2 / h ^-1 ) UV irradiation and low temperature treatment at 1 ° C ~ 10 ° C, respectively.

As a result, it was confirmed that the leaf is induced to a red color through various stress treatment as shown in FIG. In the natural state, it took about 60 days to change from green to red leaves, whereas the stress treatment took about 20 days to induce red leaves.

< Example  2> stressed Sentedgrass  Meicin and Meisin Derivative Content

The leaves of the stress treated centipedes grass were collected and used at -70 ° C. After grinding with a blender by adding 100% methanol, the extract obtained by immersion at room temperature for 3 days was concentrated under reduced pressure at 50 ℃. The sample concentrated under reduced pressure was dissolved in 100% methanol at a concentration of 10 mg / ml. The extract was measured for content of Meissin and Meisine derivatives using an Agilent HPLC (Agilent Technological, USA) instrument. The column used Beckman Coulter ULTRASPHERE (5 μm, 4.6 mm ± 25 cm) at which time the temperature of the column was adjusted to 40 ° C. HPLC was performed using a mobile phase containing a solution containing 0.1% trifluoroacetic acid (TFA) and a solution containing 50% acetonitrile (B) containing 0.1% TFA. Mobile phase conditions are shown in Table 1 and maintained at a flow rate of 1 ml / min for 50 minutes. LC-MS analysis of materials separated from the column was performed using a quadrupole mass spectrometer detector (HP1100LC-MSD, Agilent Technologies, Canada). Meicin and macine derivatives were measured at 360 nm.

Elution Method (Slope Conditions) Elution time (minutes) A: 0.1% TFA B: 0.1% TFA added
50% acetonitrile solution 0 80% 20% 18 67% 33% 30 40% 40% 40 40% 40% 42 80% 20% 50 80% 20%

As a result, as shown in FIG. 2, the content of maysin and maysin derivatives (isoorientin and rhamnosylisoorientin) of red leaves in a natural state not treated with stress was based on In mature green leaves, the content of mesine and macine derivatives was relatively low. In stress-induced red leaves, the content was slightly lower than that of natural red leaves, but it was more than 85%. High content of derivates of shin and mayes.

< Example  3> stressed Sentedgrass  Insecticidal effect

Feeding tests were performed on chestnut larvae, 3-4 days old, using Sentedgrass green leaves and various stress-treated red leaves as food. Wet 3MM paper was placed on a Petri dish of 150 × 15 mm size, Sentedgrass green leaves and various stress-treated red leaves were placed, and 10 larvae were tested in each experimental group. Every 2 days, new 3MM paper and leaves were replaced, and the survival rate was recorded daily during the 7-day pay trial.

As a result, as shown in FIG. 3, the survival rate of the night moth larva was lower when the red leaf induced by the stress and the red leaf induced by the stress than the mature green leaf were fed.

1 is a diagram showing the results of inducing the red leaves of centipede glass by various stress treatment.

Figure 2 is a diagram showing the results of analyzing the content of the mycin and the mycin derivative of the red leaf of Sentipedgrass by various stress treatment.

Figure 3 is a diagram confirming the insecticidal effect of Sentipedgrass leaf through a salary experiment.

Claims (14)

A method of rapidly increasing the content of Meissin and Meissin derivatives, comprising the step of treating stress conditions on mature leaves of Sentipedgrass in its natural state. The method of claim 1 wherein the mature leaf is a green leaf. The method of claim 1, wherein the mesine derivative is isoorientin or rhamnosylisoorientin. The method of claim 1, wherein the stress condition is selected from the group consisting of bending, radiation, ultraviolet radiation and low temperature treatment. 5. A method according to claim 4, wherein said folding can be performed at two thirds of the mature green leaves. The method of claim 4, wherein the radiation is any one selected from the group consisting of gamma rays, electron beams and X-rays. The method of claim 4, wherein the radiation is irradiated at a dose of 10 gy −2 kGy. 8. The method of claim 7, wherein the radiation is irradiated at a dose of 50 gy-1 kGy. 9. The method of claim 8, wherein the radiation is irradiated at a dose of 100 gy-0.5 kGy. 5. The method of claim 4, wherein said ultraviolet light is UV-B. The method of claim 4, wherein the ultraviolet rays are irradiated for 18 to 22 days at 5 minutes daily. The method of claim 11, wherein the ultraviolet light is irradiated for 20 days. The method of claim 4, wherein the low temperature treatment is carried out at 1 to 10 ℃. The method of claim 13, wherein the low temperature treatment is carried out at 4 ℃.

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