KR20100123050A - Multiple snp markers, microarray and diagnosis kit for sasang constitution - Google Patents

Abstract

PURPOSE: A SNP marker for diagnosing four constitution of Korean traditional medicine is provided to accurately and quickly diagnose the body constitution. CONSTITUTION: A SNP for diagnosing four constitution of Korean traditional medicine comprises a polynucleotide which contains 101th base and has 10 or more serial bases among polynucleotides of sequence numbers 1-124. A kit for diagnosing the four constitutions contains a microarray. A method for detecting multiple SNP comprises: a step of isolating nucleic acid sample from a test subject; a step of labeling the nucleic acid sample with fluorescence; a step of hybridizing the nucleic acid sample with the microarray; a step of determining genotype of each polynucleotide; and a step of determining body constitution by the genotype.

Description

Multiple SNP markers, microarray and diagnosis kit for Sasang constitution}

The present invention relates to a single Nucleotide Polymorphism (SNP) marker for filamentous constitution diagnosis, a microarray including the same, and a kit for filamentous constitution diagnosis using the same.

Founded in 1894 by Dr. Nesuma, ideology, he divided human constitutions into four categories: sun, cultivation, lunar and noise, and physiology, pathology, diagnostic differentiation, treatment, medication, and food intake. A new method of linking and applying it to the clinic has been proposed (Dong-Soo Suwon, 1984, Jeuma), and is currently used as an important clinical method in oriental medicine.

Traditionally, discrimination of Sasang Constitution has been performed by comprehensively using voice, appearance, body type, personality and various external biological indicators, and diagnosis is performed based on subjective judgment by skilled experts. Korean Journal of Medicine 8, 12, 1996).

On the other hand, according to the synod Suwon source, because the constitution is known to be innate and does not change throughout life, it can be said to be a biological characteristic. All biological traits in humans are expressed based on gene information, so the constitutional attributes suggested by ideology are also inherent in the traits of genes.

The genomes of all living organisms undergo spontaneous mutations that lead to genomic variations in their offspring during evolution (Gusella, Ann. Rev. Biochem. 55, 831-854, 1986). Mutations in the genome can be evolutionarily beneficial or disadvantageous or neutral to the species in question. In the case of functionally important gene mutations, there are cases in which lethal penalties are not transmitted to offspring; otherwise, DNA is inserted into most of the species, effectively leading to ancestral forms and associated with various biological traits. And coexist in the form of polymorphisms of sequence among species populations. Such polymorphisms include restriction fragment length polymorphism (RFLP), short tandem repeats (STR), and single nucleotide polymorphism (SNP).

Among them, SNPs are single nucleotide variations between individuals of the same species, and when SNPs occur in the protein coding region, defective or mutated proteins may be expressed by any of the polymorphic forms. The occurrence of SNPs in non-coding regions may result in the expression of defective or mutated proteins as a result of defective splicing, and some SNPs may have no effect on the phenotype. In humans, SNPs are known to occur once every 1,000 bp. When these SNPs affect phenotypes such as diseases, polynucleotides containing SNPs diagnose these diseases or biological expression traits. It can be used as a primer or a probe for the purpose.

The discrimination of ideological constitution, which has been traditionally performed as described above, is based on subjective judgment by a skilled expert by combining various internal and external biological indicators including the voice, appearance, body shape, and personality of the subject. In the above constitution discrimination method, the measurement of biological indicators is not precisely made through an objective and scientific method, and the criteria are determined because the criteria are dependent on the subjectivity of the judge in determining the final constitution by combining the values of the measured indicators. There are serious problems with the accuracy and reproducibility of constitution.

In order to facilitate the determination of filamentous constitution, conventionally, the physical response of the finger and the contact of gold or silver finger is measured (Korean Patent No. 10-0455996, 20-0325651 for the Republic of Korea Registered Room), or when the food or drug is in contact with the constitution. Information about the body shape by measuring the pressure of the wrist (Korean Patent Registration No. 10-0563127), or resinous muscle strength (Korean Registered Room 2020000032492), or the body's response (Korean Registered Room 20-0365859), or by analyzing information of a voice signal Extracting the method (Korean Patent Application Publication No. 10-2009-0009430, Republic of Korea Patent Registration 10-0427243) or using the pulsation on the wrist (Republic of Korea Patent Registration 10-0464806, Republic of Korea Patent Registration 10-0672083) Although there have been efforts to apply them, there is a lack of scientific basis, low measurement accuracy, and extremely low among the indicators of constitution. Judging from some of them did not improve accuracy, reproducibility, and reliability.

Recently, Sasangin diagnosis questionnaire was devised to systematically synthesize various constitution indicators to scientificize and objectify the constitution decision (Jung-Hee Yoo et al., J. Alternative and Complementary Medicine, 13 (1)). , 2007), a study to examine and examine constitution in terms of genetics to find differences and correlations between constitutions and to lay the groundwork for objectification of constitution (Cho, Dong-wook et al., Journal of Sasang Medicine 8 (12), 1996; The Journal of Sasang Constitutional Medicine 14 (2), 2002; Han, Sung-Kyu et al., Sasang Constitutional Society Journal 15 (1), 2003; Kim, Pan-Jun et al., Sasang Constitutional Medicine Journal, 18 (3), 2006).

However, these studies are valuable as an attempt to scientifically identify the genomic region associated with filamentous constitution using the genetics of filamentous constitution, but as a result, genetic markers that can be used to identify or diagnose genes that are clearly associated with filamentous constitution are found. It was not revealed.

Therefore, the present inventors have been researching to discover SNPs related to Sasang Constitution based on heredity of Sasang Constitution to improve the non-scientific, non-objective, low accuracy and reproducibility problems of the conventional Sasang Constitution diagnosis method described above. Among them, the population of a particular filamentous constitution has completed the present invention by confirming that a specific genotype has specific genotypes, and that the genotype information of the SNPs can diagnose the probability of filamentous constitution in the individual.

It is an object of the present invention to provide multiple Nucleotide Polymorphism (SNP) indicators capable of diagnosing filamentous constitution, a microarray using a probe comprising the same, and a kit for filamentous constitution diagnosis using the same.

In order to achieve the above object, the present invention is composed of 10 or more consecutive bases containing the base of the position (101th of each sequence) of each SNP (Single Nucleotide Polymorphism) in the polynucleotide consisting of SEQ ID NO: 1 to 124 Provided are multiple SNPs for filamentous constitution diagnostics, comprising one or more polynucleotides or complementary polynucleotides thereof selected from polynucleotides.

The present invention also provides a microarray for filamentous constitution comprising a polynucleotide of the SNP, a complementary polynucleotide thereof, a polynucleotide hybridizing with them, or a polypeptide encoded by the same.

In addition, the present invention provides a kit for filamentous constitution diagnosis comprising the microarray.

In addition, the present invention 1) separating the nucleic acid sample from the subject; 2) labeling the nucleic acid sample with a fluorescent material; 3) hybridizing a nucleic acid sample labeled with a fluorescent material with the microarray; 3) analyzing the reacted microarrays to determine the genotype of each polymorphic site of at least one polynucleotide selected from SEQ ID NOs: 1 to 124; And 4) provides a filamentous constitution diagnostic method comprising the step of determining the filamentous constitution of the subject using the genotype.

Since multiple SNPs according to the present invention have a specific combination of SNPs and genotypes that are specific only in certain constitutions of filamentous constitution and rarely appear in other constitutions, it is possible to effectively diagnose filamentous constitutions of individuals. In particular, based on the results of accurate measurement and analysis of genetic variation specific to each individual based on the genetic inheritance of filamentous constitution, multiple SNPs according to the present invention, a microarray using the same, and a kit for diagnosing filamentous constitution using the same Scientific, objective and accurate diagnosis of filamentous constitution is possible.

Hereinafter, the present invention will be described in more detail.

The present invention relates to polynucleotides consisting of 10 or more consecutive bases comprising a base at the position of each SNP (Single Nucleotide Polymorphism) in the polynucleotide consisting of SEQ ID NOs: 1 to 124 (101th of each sequence). Provided are multiple SNPs for filamentous constitution diagnostics, comprising one or more polynucleotides selected from or complementary polynucleotides thereof.

Polynucleotides comprising the nucleotide sequence of the SNP position represented by SEQ ID NO: 1 to 124 are as described in Table 1 below:

DbSNP registration number of SNP Polynucleotide with SNP DbSNP registration number of SNP Polynucleotide with SNP rs732509 SEQ ID NO: 1 rs324571 SEQ ID NO: 63 rs3933369 SEQ ID NO: 2 rs4561699 SEQ ID NO: 64 rs16982495 SEQ ID NO: 3 rs1566732 SEQ ID NO: 65 rs3929608 SEQ ID NO: 4 rs974676 SEQ ID NO: 66 rs7200468 SEQ ID NO: 5 rs9886510 SEQ ID NO: 67 rs891989 SEQ ID NO: 6 rs6499515 SEQ ID NO: 68 rs3744793 SEQ ID NO: 7 rs4882132 SEQ ID NO: 69 rs1558531 SEQ ID NO: 8 rs2415627 SEQ ID NO: 70 rs12022351 SEQ ID NO: 9 rs6548270 SEQ ID NO: 71 rs7841724 SEQ ID NO: 10 rs7395662 SEQ ID NO: 72 rs4947644 SEQ ID NO: 11 rs2359378 SEQ ID NO: 73 rs10835536 SEQ ID NO: 12 rs897735 SEQ ID NO: 74 rs6596224 SEQ ID NO: 13 rs12420372 SEQ ID NO: 75 rs9313235 SEQ ID NO: 14 rs2915414 SEQ ID NO: 76 rs714693 SEQ ID NO: 15 rs868243 SEQ ID NO: 77 rs4880851 SEQ ID NO: 16 rs11042702 SEQ ID NO: 78 rs7761051 SEQ ID NO: 17 rs2677112 SEQ ID NO: 79 rs7454257 SEQ ID NO: 18 rs41447647 SEQ ID NO: 80 rs4510645 SEQ ID NO: 19 rs17138409 SEQ ID NO: 81 rs512948 SEQ ID NO: 20 rs7155547 SEQ ID NO: 82 rs4282653 SEQ ID NO: 21 rs667770 SEQ ID NO: 83 rs3801534 SEQ ID NO: 22 rs4533500 SEQ ID NO: 84 rs2805543 SEQ ID NO: 23 rs1791169 SEQ ID NO: 85 rs818361 SEQ ID NO: 24 rs4882017 SEQ ID NO: 86 rs259073 SEQ ID NO: 25 rs423541 SEQ ID NO: 87 rs10928683 SEQ ID NO: 26 rs1546256 SEQ ID NO: 88 rs4937842 SEQ ID NO: 27 rs9287986 SEQ ID NO: 89 rs10055080 SEQ ID NO: 28 rs17171399 SEQ ID NO: 90 rs4573896 SEQ ID NO: 29 rs6581515 SEQ ID NO: 91 rs4555755 SEQ ID NO: 30 rs16992170 SEQ ID NO: 92 rs10040260 SEQ ID NO: 31 rs2173709 SEQ ID NO: 93 rs17517219 SEQ ID NO: 32 rs979309 SEQ ID NO: 94 rs17575938 SEQ ID NO: 33 rs12951421 SEQ ID NO: 95 rs2147897 SEQ ID NO: 34 rs3784120 SEQ ID NO: 96 rs756679 SEQ ID NO: 35 rs3843579 SEQ ID NO: 97 rs10455733 SEQ ID NO: 36 rs387873 SEQ ID NO: 98 rs1703663 SEQ ID NO: 37 rs9546127 SEQ ID NO: 99 rs7201911 SEQ ID NO: 38 rs2246872 SEQ ID NO: 100 rs1659794 SEQ ID NO: 39 rs1576166 SEQ ID NO: 101 rs4141371 SEQ ID NO: 40 rs10520501 SEQ ID NO: 102 rs6487998 SEQ ID NO: 41 rs10238431 SEQ ID NO: 103 rs11075059 SEQ ID NO: 42 rs6805864 SEQ ID NO: 104 rs10843984 SEQ ID NO: 43 rs857120 SEQ ID NO: 105 rs560084 SEQ ID NO: 44 rs2002284 SEQ ID NO: 106 rs10499695 SEQ ID NO: 45 rs425155 SEQ ID NO: 107 rs904759 SEQ ID NO: 46 rs9819987 SEQ ID NO: 108 rs1917927 SEQ ID NO: 47 rs1571125 SEQ ID NO: 109 rs4964821 SEQ ID NO: 48 rs1956537 SEQ ID NO: 110 rs1942761 SEQ ID NO: 49 rs779741 SEQ ID NO: 111 rs4358154 SEQ ID NO: 50 rs11703846 SEQ ID NO: 112 rs1667280 SEQ ID NO: 51 rs10800462 SEQ ID NO: 113 rs2418979 SEQ ID NO: 52 rs6765444 SEQ ID NO: 114 rs2257043 SEQ ID NO: 53 rs9826255 SEQ ID NO: 115 rs10140209 SEQ ID NO: 54 rs1675035 SEQ ID NO: 116 rs11039820 SEQ ID NO: 55 rs1326357 SEQ ID NO: 117 rs10838954 SEQ ID NO: 56 rs2797129 SEQ ID NO: 118 rs4325783 SEQ ID NO: 57 rs422779 SEQ ID NO: 119 rs762899 SEQ ID NO: 58 rs3935264 SEQ ID NO: 120 rs3764474 SEQ ID NO: 59 rs2202159 SEQ ID NO: 121 rs6485872 SEQ ID NO: 60 rs12481102 SEQ ID NO: 122 rs7131779 SEQ ID NO: 61 rs1913754 SEQ ID NO: 123 rs324572 SEQ ID NO: 62 rs4726423 SEQ ID NO: 124

The term 'SNP' refers to a form most present among DNA sequence polymorphisms by a single base-pair variation in DNA between individuals (about 1 kb).

The term dbSNP (Single Nucleotide Polymorphism Database) registration number of the SNP refers to the sequence and location of each of the SNPs. Those skilled in the art will be able to easily identify the position and sequence of the SNP using the registration number. The specific sequence corresponding to the rs number of the SNP registered in the NCBI's dbSNP may change slightly over time. It will be apparent to those skilled in the art that the scope of the present invention also applies to such altered sequences.

'SEQ ID NOS 1 to 124' is a polymorphic sequence including a polymorphic site. A polymorphic sequence refers to a sequence comprising a polymorphic site representing SNP in a polynucleotide sequence. The polynucleotide sequence may be DNA or RNA.

The characteristics of each SNP present in the polynucleotide including the nucleotide sequence of the SNP position represented by SEQ ID NOS: 1 to 124 are as described in the following [Table 2]:

Of SNP
dbSNP
Registration Number Polynucleotide with SNP band gene SNP Role Significance Diagnosis rs732509 SEQ ID NO: 1 1p32.3 THEA Intron 0.003742 noise rs3933369 SEQ ID NO: 2 13q12.12 intergenic 0.004374 noise rs16982495 SEQ ID NO: 3 2p24.3 intergenic 0.0007666 noise rs3929608 SEQ ID NO: 4 15q25.1 intergenic 0.000505 noise rs7200468 SEQ ID NO: 5 16p13.3 LOC440337 Promoter 0.003065 noise rs891989 SEQ ID NO: 6 5q31.3 GNPDA1 intergenic 0.002938 noise rs3744793 SEQ ID NO: 7 17q25.3 USP36 Coding exon 0.002268 noise rs1558531 SEQ ID NO: 8 12p13.31 CD163 Promoter 0.004418 noise rs12022351 SEQ ID NO: 9 1q41 DUSP10 Promoter 0.003378 noise rs7841724 SEQ ID NO: 10 8q24.23 ENSG00000169438 Intron 0.00198 noise rs4947644 SEQ ID NO: 11 7p12.2 DDC Intron 0.001133 noise rs10835536 SEQ ID NO: 12 11p14.1 intergenic 0.004287 noise rs6596224 SEQ ID NO: 13 5q31.1 H2AFY Promoter 0.001013 noise rs9313235 SEQ ID NO: 14 5p15.31 intergenic 0.0003285 noise rs714693 SEQ ID NO: 15 6q21 ENSG00000053328 Intron 0.002106 noise rs4880851 SEQ ID NO: 16 10p15.3 ADARB2 Intron 0.001461 noise rs7761051 SEQ ID NO: 17 6q23.2 MOXD1 Intron 0.001593 noise rs7454257 SEQ ID NO: 18 6q27 intergenic 0.0006561 noise rs4510645 SEQ ID NO: 19 6q27 intergenic 0.0006812 noise rs512948 SEQ ID NO: 20 13q14.3 FLJ13639 Intron 0.004322 noise rs7470823 SEQ ID NO: 21 9p13.3 OR13J1 Promoter 0.00363 noise rs3801534 SEQ ID NO: 22 7q21.11 SEMA3E Intron 0.000203 noise rs2805543 SEQ ID NO: 23 10p15.3 ADARB2 Intron 0.002202 noise rs818361 SEQ ID NO: 24 2q31.1 intergenic 0.002731 noise rs259073 SEQ ID NO: 25 5q14.1 intergenic 9.85E-05 noise rs10928683 SEQ ID NO: 26 2q22.1 ENSG00000197221 Promoter 0.0008505 noise rs4937842 SEQ ID NO: 27 11q25 SPATA19 Promoter 0.0007713 noise rs10055080 SEQ ID NO: 28 5q35.1 ENSG00000174705 Intron 0.00131 noise rs6416513 SEQ ID NO: 29 15q25.1 intergenic 0.0004234 noise rs4555755 SEQ ID NO: 30 5p15.31 intergenic 0.002847 noise rs10040260 SEQ ID NO: 31 5p15.33 FLJ12443 Promoter 0.001038 noise rs17517219 SEQ ID NO: 32 2p12 intergenic 0.0001654 noise rs17575938 SEQ ID NO: 33 2p12 intergenic 0.002975 noise rs2147897 SEQ ID NO: 34 10q24.2 C10orf33 Intron 0.001347 noise rs756679 SEQ ID NO: 35 9q34.2 BRD3 Promoter 0.001111 noise rs10455733 SEQ ID NO: 36 6q27 intergenic 0.002755 noise rs1703663 SEQ ID NO: 37 10q26.3 intergenic 0.0002188 noise rs7201911 SEQ ID NO: 38 16p13.3 LOC440337 Promoter 0.00162 noise rs1659794 SEQ ID NO: 39 7q34 LOC136306 Intron 0.001612 noise rs4141371 SEQ ID NO: 40 7p21.1 ABCB5 Promoter 0.004626 noise rs6487998 SEQ ID NO: 41 12p11.21 ENSG00000170456 Intron 0.0007071 noise rs11075059 SEQ ID NO: 42 16p13.13 ENSG00000048471 Intron 0.00435 noise rs10843984 SEQ ID NO: 43 12p11.21 ENSG00000170456 Intron 0.002297 noise rs560084 SEQ ID NO: 44 6q21 ARMC2 Promoter 0.002864 noise rs10499695 SEQ ID NO: 45 7p12.2 DDC Intron 0.003035 noise rs904759 SEQ ID NO: 46 5p15.33 FLJ12443 Promoter 0.001431 noise rs1917927 SEQ ID NO: 47 18p11.31 EPB41L3 Promoter 0.001469 noise rs4964821 SEQ ID NO: 48 12q23.3 CHST11 Intron 0.001557 noise rs1942761 SEQ ID NO: 49 18q22.3 NETO1 Promoter 0.002101 quality rs4358154 SEQ ID NO: 50 2p25.3 SNTG2 Promoter 0.001595 quality rs1667280 SEQ ID NO: 51 18q12.1 B4GALT6 Intron 0.002805 quality rs2418979 SEQ ID NO: 52 10q25.1 SORCS1 Promoter 0.004352 quality rs2257043 SEQ ID NO: 53 7q31.1 LRRN3 Promoter 0.004366 quality rs10140209 SEQ ID NO: 54 14q21.1 LRFN5 Promoter 0.003886 quality rs11039820 SEQ ID NO: 55 11p11.2 ENSG00000166013 Promoter 0.002235 quality rs10838954 SEQ ID NO: 56 11p11.2 ENSG00000166013 Promoter 0.002673 quality rs4325783 SEQ ID NO: 57 2p25.3 SNTG2 Promoter 0.001595 quality rs762899 SEQ ID NO: 58 22q12.3 SYN3 Intron 0.002214 quality rs3764474 SEQ ID NO: 59 18q12.1 B4GALT6 Intron 0.003267 quality rs6485872 SEQ ID NO: 60 11p11.2 ENSG00000166013 Promoter 0.002235 quality rs7131779 SEQ ID NO: 61 12q12 NELL2 Promoter 0.003422 quality rs324572 SEQ ID NO: 62 7q33 intergenic 0.001081 quality rs324571 SEQ ID NO: 63 7q33 intergenic 0.001081 quality rs4561699 SEQ ID NO: 64 2p25.3 SNTG2 Promoter 0.000402 quality rs1566732 SEQ ID NO: 65 11p11.2 PTPRJ Intron 8.45E-05 quality rs974676 SEQ ID NO: 66 18q12.1 intergenic 0.004622 quality rs12707894 SEQ ID NO: 67 8q24.13 HAS2 Promoter 0.001131 quality rs6499515 SEQ ID NO: 68 16q22.2 CALB2 Intron 0.00422 quality rs6485884 SEQ ID NO: 69 11p11.2 ENSG00000166013 Promoter 0.002235 quality rs2415627 SEQ ID NO: 70 14q21.1 LRFN5 Promoter 0.003886 quality rs6548270 SEQ ID NO: 71 2p25.3 SNTG2 Promoter 0.0002715 quality rs7395662 SEQ ID NO: 72 11p11.2 ENSG00000166013 Promoter 0.002235 quality rs2359378 SEQ ID NO: 73 1q31.3 NEK7 Promoter 0.001592 quality rs897735 SEQ ID NO: 74 8q12.1 ENSG00000172659 Promoter 0.0001572 quality rs12420372 SEQ ID NO: 75 11p11.2 OR4A47 Promoter 0.002235 quality rs2915414 SEQ ID NO: 76 9q31.1 PRG-3 Intron 0.001584 quality rs868243 SEQ ID NO: 77 3p26.1 GRM7 Promoter 0.003708 quality rs11042702 SEQ ID NO: 78 11p15.4 SBF2 Intron 0.003857 quality rs2677112 SEQ ID NO: 79 1q41 USH2A Intron 0.001512 quality rs41447647 SEQ ID NO: 80 18q12.1 B4GALT6 Promoter 0.004409 quality rs17138409 SEQ ID NO: 81 5q23.1 FLJ90650 Promoter 0.001504 quality rs7155547 SEQ ID NO: 82 14q21.1 LRFN5 Promoter 0.004665 quality rs667770 SEQ ID NO: 83 1p12 PHGDH Intron 0.003116 quality rs4533500 SEQ ID NO: 84 2p25.3 SNTG2 Promoter 0.001682 quality rs1791169 SEQ ID NO: 85 18q12.1 B4GALT6 Intron 0.002938 quality rs4882017 SEQ ID NO: 86 11p11.2 ENSG00000166013 Promoter 0.002235 quality rs423541 SEQ ID NO: 87 4q32.3 intergenic 0.0009464 Lunar rs1546256 SEQ ID NO: 88 9p24.1 C9orf123 Promoter 0.001395 Lunar rs9287986 SEQ ID NO: 89 2q31.1 ATP5G3 Promoter 0.002659 Lunar rs17171399 SEQ ID NO: 90 7p14.1 AMPH Intron 0.002887 Lunar rs6581515 SEQ ID NO: 91 12q14.2 SRGAP1 Intron 0.001487 Lunar rs16992170 SEQ ID NO: 92 20q13.12 EYA2 Intron 0.003591 Lunar rs2173709 SEQ ID NO: 93 9p24.1 C9orf123 Promoter 0.001395 Lunar rs979309 SEQ ID NO: 94 2p24.3 FAM84A Promoter 0.003336 Lunar rs12951421 SEQ ID NO: 95 17p12 FLJ45455 Promoter 0.004103 Lunar rs3784120 SEQ ID NO: 96 14q24.1 RAD51L1 Intron 0.003016 Lunar rs3843579 SEQ ID NO: 97 9p24.1 C9orf123 Promoter 0.0001622 Lunar rs387873 SEQ ID NO: 98 4q32.3 intergenic 0.0007655 Lunar rs9546127 SEQ ID NO: 99 13q31.1 SPRY2 Promoter 0.00373 Lunar rs2246872 SEQ ID NO: 100 4q28.3 intergenic 0.004487 Lunar rs1576166 SEQ ID NO: 101 13q33.3 intergenic 0.003829 Lunar rs10520501 SEQ ID NO: 102 4q34.3 ENSG00000197331 Promoter 0.001602 Lunar rs10238431 SEQ ID NO: 103 7q21.11 PCLO Promoter 0.0008254 Lunar rs6805864 SEQ ID NO: 104 3p14.3 SPATA12 Promoter 0.00305 Lunar rs857120 SEQ ID NO: 105 1p32.2 C1orf168 Intron 0.003569 Lunar rs2002284 SEQ ID NO: 106 9q32 KIF12 Promoter 0.002019 Lunar rs7376151 SEQ ID NO: 107 4q32.3 intergenic 0.0007655 Lunar rs9819987 SEQ ID NO: 108 3p26.1 GRM7 Intron 0.002545 Lunar rs1571125 SEQ ID NO: 109 9q21.13 PCSK5 Promoter 0.001427 Lunar rs1956537 SEQ ID NO: 110 14q24.1 RAD51L1 Intron 0.002269 Lunar rs779741 SEQ ID NO: 111 3p26.1 GRM7 Intron 0.002482 Lunar rs11703846 SEQ ID NO: 112 22q13.31 intergenic 0.004504 Lunar rs10800462 SEQ ID NO: 113 1q24.2 SELP Promoter 0.0003808 Lunar rs6765444 SEQ ID NO: 114 3p14.3 ARHGEF3 Coding exon 0.00305 Lunar rs9826255 SEQ ID NO: 115 3p14.3 RAP140 Promoter 0.00305 Lunar rs1675035 SEQ ID NO: 116 4q32.3 intergenic 0.0007699 Lunar rs1326357 SEQ ID NO: 117 10q25.1 intergenic 0.004251 Lunar rs2797129 SEQ ID NO: 118 10p11.22 intergenic 0.004436 Lunar rs422779 SEQ ID NO: 119 4q32.3 intergenic 0.001322 Lunar rs3935264 SEQ ID NO: 120 2q31.1 ATP5G3 Promoter 0.002508 Lunar rs2202159 SEQ ID NO: 121 15q14 SPRED1 Promoter 0.004513 Lunar rs12481102 SEQ ID NO: 122 20q13.12 EYA2 Intron 0.0005227 Lunar rs1913754 SEQ ID NO: 123 10q22.3 NRG3 Promoter 0.003427 Lunar rs4726423 SEQ ID NO: 124 7q36.2 DPP6 Intron 0.001597 Lunar

In [Table 2], the term 'band' means a chromosome number where each single SNP is located, a short arm (p) portion or a long arm (q) portion and a band number centered on a chromosome on each chromosome. do. For example, the position of the SNP included in SEQ ID NO: 1 is 1p32.3, which means that it is on the 32.3 band of the forearm portion (p) on chromosome 1.

The term 'gene' means a gene comprising each SNP.

The term 'role of SNP' refers to the role of each single SNP in the gene.

The term 'diagnosis constitution' is a constitution that can be used for diagnosis using each SNP. For example, the SNP corresponding to SEQ ID NO: 1 can be used to diagnose noise constitution.

The term 'significance probability' refers to the probability that each SNP is not related to a specific constitution in filamentous constitution, and the genotype distribution actually tested under the null hypothesis that the genotype distribution of each SNP is uniform between a specific constitution group and the rest of the constitution group The probability to be obtained is calculated by the chi-square test. In other words, the smaller the value of the probability of significance, the more closely the SNP is related to the constitution. For example, under the assumption that the SNP corresponding to SEQ ID NO: 1 in Table 2 is not related to the noise constitution, the probability of the experimentally obtained genotypic distribution is about 0.004, which corresponds to SEQ ID NO: 1 It can be seen that SNP is deeply related to noise constitution. As shown in [Table 2], SNPs corresponding to SEQ ID NOs: 1 to 124 have a low probability ranging from 0.00008 to 0.005, and the SNPs are deeply related to filamentous constitutions corresponding to each diagnosis. It can be expected that the genotype information of the SNPs can be effectively used for the diagnosis of filamentous constitution.

The term chi-square test is a statistical test used in a crosstab to evaluate the association between two variables. Under the null hypothesis that the two variables are not related, a group of specific SNPs shown in Table 3 The chi-square statistic, calculated from Equation 1 from the cross-partition table for star genotype frequencies, uses the chi-square distribution with degrees of freedom (number of partition columns-1) × (number of partition lines-1). Statistical test to calculate the probability of significance.

Genotype Genotype Observed Genotype Expected Constitution of interest Other constitution all Constitution of interest Other constitution all AA O ₁₁ O ₁₂ n ₁₊ E ₁₁ E ₁₂ n ₁₊ AB O ₂₁ O ₂₂ n ₂₊ E ₂₁ E ₂₂ n ₂₊ BB O ₃₁ O ₃₂ n ₃₊ E ₃₁ E ₃₂ n ₃₊ all n ₊₁ n ₊₂ n n ₊₁ n ₊₂ n

In the multiple SNP for filamentous constitution diagnosis according to the present invention, the one or more polynucleotides are 10 or more consecutive bases including the position (101st of each sequence) base of each SNP in the polynucleotides consisting of SEQ ID NOs: 1 to 124 From the polynucleotide consisting of, it is preferably selected from the group consisting of 1 to 108 selected from the combination of Table 4 below.

number Multi SNP Diagnosis One SEQ ID NO: 17, SEQ ID NO: 32, SEQ ID NO: 41 noise 2 SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 34 noise 3 SEQ ID NO: 17, SEQ ID NO: 33, SEQ ID NO: 41 noise 4 SEQ ID NO: 4, SEQ ID NO: 27, SEQ ID NO: 35 noise 5 SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 35 noise 6 SEQ ID NO: 2, SEQ ID NO: 39, SEQ ID NO: 45 noise 7 SEQ ID NO: 22, SEQ ID NO: 34, SEQ ID NO: 42 noise 8 SEQ ID NO: 10, SEQ ID NO: 13, SEQ ID NO: 41 noise 9 SEQ ID NO: 8, SEQ ID NO: 22, SEQ ID NO: 42 noise 10 SEQ ID NO: 2, SEQ ID NO: 7, SEQ ID NO: 47 noise 11 SEQ ID NO: 6, SEQ ID NO: 12, SEQ ID NO: 20 noise 12 SEQ ID NO: 10, SEQ ID NO: 15, SEQ ID NO: 25 noise 13 SEQ ID NO: 10, SEQ ID NO: 13, SEQ ID NO: 43 noise 14 SEQ ID NO: 2, SEQ ID NO: 11, SEQ ID NO: 39 noise 15 SEQ ID NO: 9, SEQ ID NO: 22, SEQ ID NO: 42 noise 16 SEQ ID NO: 27, SEQ ID NO: 30, SEQ ID NO: 44 noise 17 SEQ ID NO: 14, SEQ ID NO: 27, SEQ ID NO: 44 noise 18 SEQ ID NO: 10, SEQ ID NO: 13, SEQ ID NO: 34 noise 19 SEQ ID NO: 18, SEQ ID NO: 22, SEQ ID NO: 34 noise 20 SEQ ID NO: 19, SEQ ID NO: 22, SEQ ID NO: 34 noise 21 SEQ ID NO: 6, SEQ ID NO: 20, SEQ ID NO: 18 noise 22 SEQ ID NO: 6, SEQ ID NO: 20, SEQ ID NO: 19 noise 23 SEQ ID NO: 22, SEQ ID NO: 34, SEQ ID NO: 36 noise 24 SEQ ID NO: 3, SEQ ID NO: 10, SEQ ID NO: 13 noise 25 SEQ ID NO: 28, SEQ ID NO: 31, SEQ ID NO: 34 noise 26 SEQ ID NO: 19, SEQ ID NO: 37, SEQ ID NO: 48 noise 27 SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40 noise 28 SEQ ID NO: 18, SEQ ID NO: 37, SEQ ID NO: 48 noise 29 SEQ ID NO: 5, SEQ ID NO: 39, SEQ ID NO: 40 noise 30 SEQ ID NO: 28, SEQ ID NO: 34, SEQ ID NO: 46 noise 31 SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 26 noise 32 SEQ ID NO: 1, SEQ ID NO: 34, SEQ ID NO: 48 noise 33 SEQ ID NO: 16, SEQ ID NO: 22, SEQ ID NO: 34 noise 34 SEQ ID NO: 16, SEQ ID NO: 24, SEQ ID NO: 26 noise 35 SEQ ID NO: 61, SEQ ID NO: 62, SEQ ID NO: 76 quality 36 SEQ ID NO: 61, SEQ ID NO: 63, SEQ ID NO: 76 quality 37 SEQ ID NO: 54, SEQ ID NO: 64, SEQ ID NO: 67 quality 38 SEQ ID NO: 64, SEQ ID NO: 67, SEQ ID NO: 70 quality 39 SEQ ID NO: 64, SEQ ID NO: 67, SEQ ID NO: 82 quality 40 SEQ ID NO: 51, SEQ ID NO: 65, SEQ ID NO: 79 quality 41 SEQ ID NO: 65, SEQ ID NO: 79, SEQ ID NO: 85 quality 42 SEQ ID NO: 65, SEQ ID NO: 66, SEQ ID NO: 79 quality 43 SEQ ID NO: 65, SEQ ID NO: 79, SEQ ID NO: 80 quality 44 SEQ ID NO: 59, SEQ ID NO: 65, SEQ ID NO: 79 quality 45 SEQ ID NO: 54, SEQ ID NO: 71, SEQ ID NO: 78 quality 46 SEQ ID NO: 70, SEQ ID NO: 71, SEQ ID NO: 78 quality 47 SEQ ID NO: 71, SEQ ID NO: 78, SEQ ID NO: 82 quality 48 SEQ ID NO: 69, SEQ ID NO: 79, SEQ ID NO: 85 quality 49 SEQ ID NO: 66, SEQ ID NO: 69, SEQ ID NO: 79 quality 50 SEQ ID NO: 51, SEQ ID NO: 69, SEQ ID NO: 79 quality 51 SEQ ID NO: 69, SEQ ID NO: 79, SEQ ID NO: 80 quality 52 SEQ ID NO: 50, SEQ ID NO: 54, SEQ ID NO: 78 quality 53 SEQ ID NO: 50, SEQ ID NO: 70, SEQ ID NO: 78 quality 54 SEQ ID NO: 54, SEQ ID NO: 57, SEQ ID NO: 78 quality 55 SEQ ID NO: 57, SEQ ID NO: 70, SEQ ID NO: 78 quality 56 SEQ ID NO: 59, SEQ ID NO: 69, SEQ ID NO: 79 quality 57 SEQ ID NO: 50, SEQ ID NO: 78, SEQ ID NO: 82 quality 58 SEQ ID NO: 57, SEQ ID NO: 78, SEQ ID NO: 82 quality 59 SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 56 quality 60 SEQ ID NO: 79, SEQ ID NO: 75, SEQ ID NO: 80 quality 61 SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 55 quality 62 SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 60 quality 63 SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 72 quality 64 SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 86 quality 65 SEQ ID NO: 51, SEQ ID NO: 74, SEQ ID NO: 79 quality 66 SEQ ID NO: 53, SEQ ID NO: 77, SEQ ID NO: 83 quality 67 SEQ ID NO: 49, SEQ ID NO: 73, SEQ ID NO: 83 quality 68 SEQ ID NO: 54, SEQ ID NO: 78, SEQ ID NO: 84 quality 69 SEQ ID NO: 70, SEQ ID NO: 78, SEQ ID NO: 84 quality 70 SEQ ID NO: 78, SEQ ID NO: 82, SEQ ID NO: 84 quality 71 SEQ ID NO: 52, SEQ ID NO: 68, SEQ ID NO: 81 quality 72 SEQ ID NO: 58, SEQ ID NO: 73, SEQ ID NO: 83 quality 73 SEQ ID NO: 91, SEQ ID NO: 95, SEQ ID NO: 100 Lunar 74 SEQ ID NO: 117, SEQ ID NO: 120, SEQ ID NO: 122 Lunar 75 SEQ ID NO: 91, SEQ ID NO: 98, SEQ ID NO: 103 Lunar 76 SEQ ID NO: 91, SEQ ID NO: 103, SEQ ID NO: 107 Lunar 77 SEQ ID NO: 89, SEQ ID NO: 94, SEQ ID NO: 101 Lunar 78 SEQ ID NO: 91, SEQ ID NO: 103, SEQ ID NO: 116 Lunar 79 SEQ ID NO: 91, SEQ ID NO: 103, SEQ ID NO: 119 Lunar 80 SEQ ID NO: 111, SEQ ID NO: 118, SEQ ID NO: 122 Lunar 81 SEQ ID NO: 104, SEQ ID NO: 111, SEQ ID NO: 113 Lunar 82 SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 114 Lunar 83 SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115 Lunar 84 SEQ ID NO: 105, SEQ ID NO: 113, SEQ ID NO: 123 Lunar 85 SEQ ID NO: 88, SEQ ID NO: 104, SEQ ID NO: 124 Lunar 86 SEQ ID NO: 88, SEQ ID NO: 114, SEQ ID NO: 124 Lunar 87 SEQ ID NO: 88, SEQ ID NO: 115, SEQ ID NO: 124 Lunar 88 SEQ ID NO: 93, SEQ ID NO: 104, SEQ ID NO: 124 Lunar 89 SEQ ID NO: 93, SEQ ID NO: 114, SEQ ID NO: 124 Lunar 90 SEQ ID NO: 93, SEQ ID NO: 115, SEQ ID NO: 124 Lunar 91 SEQ ID NO: 94, SEQ ID NO: 103, SEQ ID NO: 106 Lunar 92 SEQ ID NO: 109, SEQ ID NO: 110, SEQ ID NO: 121 Lunar 93 SEQ ID NO: 87, SEQ ID NO: 99, SEQ ID NO: 104 Lunar 94 SEQ ID NO: 87, SEQ ID NO: 99, SEQ ID NO: 114 Lunar 95 SEQ ID NO: 87, SEQ ID NO: 99, SEQ ID NO: 115 Lunar 96 SEQ ID NO: 92, SEQ ID NO: 111, SEQ ID NO: 118 Lunar 97 SEQ ID NO: 96, SEQ ID NO: 109, SEQ ID NO: 121 Lunar 98 SEQ ID NO: 97, SEQ ID NO: 104, SEQ ID NO: 124 Lunar 99 SEQ ID NO: 97, SEQ ID NO: 114, SEQ ID NO: 124 Lunar 100 SEQ ID NO: 97, SEQ ID NO: 115, SEQ ID NO: 124 Lunar 101 SEQ ID NO: 107, SEQ ID NO: 99, SEQ ID NO: 104 Lunar 102 SEQ ID NO: 107, SEQ ID NO: 99, SEQ ID NO: 114 Lunar 103 SEQ ID NO: 107, SEQ ID NO: 99, SEQ ID NO: 115 Lunar 104 SEQ ID NO: 114, SEQ ID NO: 98, SEQ ID NO: 99 Lunar 105 SEQ ID NO: 115, SEQ ID NO: 98, SEQ ID NO: 99 Lunar 106 SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO: 122 Lunar 107 SEQ ID NO: 90, SEQ ID NO: 102, SEQ ID NO: 103 Lunar 108 SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 120 Lunar

The 'diagnosis constitution' is one of noise, literacy, lume, constitution, which can be diagnosed using the genotype of 'multiple SNP'.

In the multiple SNP for filamentous constitution diagnosing according to the present invention, the allele type of each SNP position (101st of each sequence) base of the selected polynucleotides is preferably the allele type shown in Table 5.

number Multi SNP Allele type Diagnosis One (SEQ ID NO: 17, SEQ ID NO: 32, SEQ ID NO: 41) (AA, AA, AG) noise 2 (SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 34) (GG, GG, AA) noise 3 (SEQ ID NO: 17, SEQ ID NO: 33, SEQ ID NO: 41) (AA, AA, AG) noise 4 (SEQ ID NO: 4, SEQ ID NO: 27, SEQ ID NO: 35) (AA, CC, CC) noise 5 (SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 35) (CC, AA, CC) noise 6 (SEQ ID NO: 2, SEQ ID NO: 39, SEQ ID NO: 45) (CC, TT, AA) noise 7 (SEQ ID NO: 22, SEQ ID NO: 34, SEQ ID NO: 42) (GG, AA, GG) noise 8 (SEQ ID NO: 10, SEQ ID NO: 13, SEQ ID NO: 41) (GG, AA, AG) noise 9 (SEQ ID NO: 8, SEQ ID NO: 22, SEQ ID NO: 42) (CC, GG, GG) noise 10 (SEQ ID NO: 2, SEQ ID NO: 7, SEQ ID NO: 47) (CC, TT, GG) noise 11 (SEQ ID NO: 6, SEQ ID NO: 12, SEQ ID NO: 20) (GG, AA, AA) noise 12 (SEQ ID NO: 10, SEQ ID NO: 15, SEQ ID NO: 25) (GG, AA, TT) noise 13 (SEQ ID NO: 10, SEQ ID NO: 13, SEQ ID NO: 43) (GG, AA, CT) noise 14 (SEQ ID NO: 2, SEQ ID NO: 11, SEQ ID NO: 39) (CC, TT, TT) noise 15 (SEQ ID NO: 9, SEQ ID NO: 22, SEQ ID NO: 42) (TT, GG, GG) noise 16 (SEQ ID NO: 27, SEQ ID NO: 30, SEQ ID NO: 44) (CC, CC, CC) noise 17 (SEQ ID NO: 14, SEQ ID NO: 27, SEQ ID NO: 44) (CC, CC, CC) noise 18 (SEQ ID NO: 10, SEQ ID NO: 13, SEQ ID NO: 34) (GG, AA, AA) noise 19 (SEQ ID NO: 18, SEQ ID NO: 22, SEQ ID NO: 34) (AA, GG, AA) noise 20 (SEQ ID NO: 19, SEQ ID NO: 22, SEQ ID NO: 34) (CC, GG, AA) noise 21 (SEQ ID NO: 6, SEQ ID NO: 20, SEQ ID NO: 18) (GG, AA, AA) noise 22 (SEQ ID NO: 6, SEQ ID NO: 20, SEQ ID NO: 19) (GG, AA, CC) noise 23 (SEQ ID NO: 22, SEQ ID NO: 34, SEQ ID NO: 36) (GG, AA, CC) noise 24 (SEQ ID NO: 3, SEQ ID NO: 10, SEQ ID NO: 13) (AG, GG, AA) noise 25 (SEQ ID NO 28, SEQ ID NO: 31, SEQ ID NO: 34) (AA, AA, AA) noise 26 (SEQ ID NO: 19, SEQ ID NO: 37, SEQ ID NO: 48) (CC, CC, GG) noise 27 (SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40) (GG, TT, AG) noise 28 (SEQ ID NO: 18, SEQ ID NO: 37, SEQ ID NO: 48) (AA, CC, GG) noise 29 (SEQ ID NO: 5, SEQ ID NO: 39, SEQ ID NO: 40) (GG, TT, AG) noise 30 (SEQ ID NO 28, SEQ ID NO: 34, SEQ ID NO: 46) (AA, AA, GG) noise 31 (SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 26) (CC, AA, CC) noise 32 (SEQ ID NO: 1, SEQ ID NO: 34, SEQ ID NO: 48) (TT, AA, GG) noise 33 (SEQ ID NO: 16, SEQ ID NO: 22, SEQ ID NO: 34) (GG, GG, AA) noise 34 (SEQ ID NO: 16, SEQ ID NO: 24, SEQ ID NO: 26) (GG, AA, CC) noise 35 (SEQ ID NO: 61, SEQ ID NO: 62, SEQ ID NO: 76) (AT, CC, CC) quality 36 (SEQ ID NO: 61, SEQ ID NO: 63, SEQ ID NO: 76) (AT, GG, CC) quality 37 (SEQ ID NO: 54, SEQ ID NO: 64, SEQ ID NO: 67) (TT, GG, CT) quality 38 (SEQ ID NO: 64, SEQ ID NO: 67, SEQ ID NO: 70) (GG, CT, GG) quality 39 (SEQ ID NO: 64, SEQ ID NO: 67, SEQ ID NO: 82) (GG, CT, TT) quality 40 (SEQ ID NO: 51, SEQ ID NO: 65, SEQ ID NO: 79) (TT, AA, AA) quality 41 (SEQ ID NO: 65, SEQ ID NO: 79, SEQ ID NO: 85) (AA, AA, TT) quality 42 (SEQ ID NO: 65, SEQ ID NO: 66, SEQ ID NO: 79) (AA, TT, AA) quality 43 (SEQ ID NO: 65, SEQ ID NO: 79, SEQ ID NO: 80) (AA, AA, TT) quality 44 (SEQ ID NO: 59, SEQ ID NO: 65, SEQ ID NO: 79) (GG, AA, AA) quality 45 (SEQ ID NO 54, SEQ ID NO 71, SEQ ID NO 78) (TT, GG, AG) quality 46 (SEQ ID NO: 70, SEQ ID NO: 71, SEQ ID NO: 78) (GG, GG, AG) quality 47 (SEQ ID NO: 71, SEQ ID NO: 78, SEQ ID NO: 82) (GG, AG, TT) quality 48 (SEQ ID NO: 69, SEQ ID NO: 79, SEQ ID NO: 85) (TT, AA, TT) quality 49 (SEQ ID NO: 66, SEQ ID NO: 69, SEQ ID NO: 79) (TT, TT, AA) quality 50 (SEQ ID NO: 51, SEQ ID NO: 69, SEQ ID NO: 79) (TT, TT, AA) quality 51 (SEQ ID NO: 69, SEQ ID NO: 79, SEQ ID NO: 80) (TT, AA, TT) quality 52 (SEQ ID NO: 50, SEQ ID NO: 54, SEQ ID NO: 78) (AA, TT, AG) quality 53 (SEQ ID NO: 50, SEQ ID NO: 70, SEQ ID NO: 78) (AA, GG, AG) quality 54 (SEQ ID NO 54, SEQ ID NO 57, SEQ ID NO 78) (TT, GG, AG) quality 55 (SEQ ID NO: 57, SEQ ID NO: 70, SEQ ID NO: 78) (GG, GG, AG) quality 56 (SEQ ID NO: 59, SEQ ID NO: 69, SEQ ID NO: 79) (GG, TT, AA) quality 57 (SEQ ID NO: 50, SEQ ID NO: 78, SEQ ID NO: 82) (AA, AG, TT) quality 58 (SEQ ID NO: 57, SEQ ID NO: 78, SEQ ID NO: 82) (GG, AG, TT) quality 59 (SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 56) (AA, TT, TT) quality 60 (SEQ ID NO: 79, SEQ ID NO: 75, SEQ ID NO: 80) (AA, AA, TT) quality 61 (SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 55) (AA, TT, GG) quality 62 (SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 60) (AA, TT, CC) quality 63 (SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 72) (AA, TT, CC) quality 64 (SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 86) (AA, TT, CC) quality 65 (SEQ ID NO: 51, SEQ ID NO: 74, SEQ ID NO: 79) (TT, GG, AA) quality 66 (SEQ ID NO: 53, SEQ ID NO: 77, SEQ ID NO: 83) (AG, CC, CC) quality 67 (SEQ ID NO: 49, SEQ ID NO: 73, SEQ ID NO: 83) (AA, AA, TT) quality 68 (SEQ ID NO: 54, SEQ ID NO: 78, SEQ ID NO: 84) (TT, AG, AA) quality 69 (SEQ ID NO: 70, SEQ ID NO: 78, SEQ ID NO: 84) (GG, AG, AA) quality 70 (SEQ ID NO: 78, SEQ ID NO: 82, SEQ ID NO: 84) (AG, TT, AA) quality 71 (SEQ ID NO: 52, SEQ ID NO: 68, SEQ ID NO: 81) (TT, CT, CC) quality 72 (SEQ ID NO 58, SEQ ID NO: 73, SEQ ID NO: 83) (CG, AA, TT) quality 73 (SEQ ID NO: 91, SEQ ID NO: 95, SEQ ID NO: 100) (TT, CT, CG) Lunar 74 (SEQ ID NO: 117, SEQ ID NO: 120, SEQ ID NO: 122) (AG, CC, GG) Lunar 75 (SEQ ID NO: 91, SEQ ID NO: 98, SEQ ID NO: 103) (TT, AT, AA) Lunar 76 (SEQ ID NO: 91, SEQ ID NO: 103, SEQ ID NO: 107) (TT, AA, AG) Lunar 77 (SEQ ID NO: 89, SEQ ID NO: 94, SEQ ID NO: 101) (AG, AG, AA) Lunar 78 (SEQ ID NO: 91, SEQ ID NO: 103, SEQ ID NO: 116) (TT, AA, AC) Lunar 79 (SEQ ID NO: 91, SEQ ID NO: 103, SEQ ID NO: 119) (TT, AA, AT) Lunar 80 (SEQ ID NO: 111, SEQ ID NO: 118, SEQ ID NO: 122) (CC, AG, GG) Lunar 81 (SEQ ID NO: 104, SEQ ID NO: 111, SEQ ID NO: 113) (GG, CC, AA) Lunar 82 (SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 114) (CC, AA, TT) Lunar 83 (SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115) (CC, AA, CC) Lunar 84 (SEQ ID NO: 105, SEQ ID NO: 113, SEQ ID NO: 123) (AG, AA, AG) Lunar 85 (SEQ ID NO: 88, SEQ ID NO: 104, SEQ ID NO: 124) (TT, GG, AG) Lunar 86 (SEQ ID NO: 88, SEQ ID NO: 114, SEQ ID NO: 124) (TT, TT, AG) Lunar 87 (SEQ ID NO: 88, SEQ ID NO: 115, SEQ ID NO: 124) (TT, CC, AG) Lunar 88 (SEQ ID NO: 93, SEQ ID NO: 104, SEQ ID NO: 124) (CC, GG, AG) Lunar 89 (SEQ ID NO: 93, SEQ ID NO: 114, SEQ ID NO: 124) (CC, TT, AG) Lunar 90 (SEQ ID NO: 93, SEQ ID NO: 115, SEQ ID NO: 124) (CC, CC, AG) Lunar 91 (SEQ ID NO: 94, SEQ ID NO: 103, SEQ ID NO: 106) (AG, AA, CC) Lunar 92 (SEQ ID NO: 109, SEQ ID NO: 110, SEQ ID NO: 121) (CT, CC, TT) Lunar 93 (SEQ ID NO: 87, SEQ ID NO: 99, SEQ ID NO: 104) (AG, AA, GG) Lunar 94 (SEQ ID NO: 87, SEQ ID NO: 99, SEQ ID NO: 114) (AG, AA, TT) Lunar 95 (SEQ ID NO: 87, SEQ ID NO: 99, SEQ ID NO: 115) (AG, AA, CC) Lunar 96 (SEQ ID NO: 92, SEQ ID NO: 111, SEQ ID NO: 118) (CC, CC, AG) Lunar 97 (SEQ ID NO: 96, SEQ ID NO: 109, SEQ ID NO: 121) (CC, CT, TT) Lunar 98 (SEQ ID NO: 97, SEQ ID NO: 104, SEQ ID NO: 124) (CC, GG, AG) Lunar 99 (SEQ ID NO: 97, SEQ ID NO: 114, SEQ ID NO: 124) (CC, TT, AG) Lunar 100 (SEQ ID NO: 97, SEQ ID NO: 115, SEQ ID NO: 124) (CC, CC, AG) Lunar 101 (SEQ ID NO: 107, SEQ ID NO: 99, SEQ ID NO: 104) (AG, AA, GG) Lunar 102 (SEQ ID NO: 107, SEQ ID NO: 99, SEQ ID NO: 114) (AG, AA, TT) Lunar 103 (SEQ ID NO: 107, SEQ ID NO: 99, SEQ ID NO: 115) (AG, AA, CC) Lunar 104 (SEQ ID NO: 114, SEQ ID NO: 98, SEQ ID NO: 99) (TT, AT, AA) Lunar 105 (SEQ ID NO: 115, SEQ ID NO: 98, SEQ ID NO: 99) (CC, AT, AA) Lunar 106 (SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO: 122) (CC, CT, GG) Lunar 107 (SEQ ID NO: 90, SEQ ID NO: 102, SEQ ID NO: 103) (AA, GG, AA) Lunar 108 (SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 120) (GT, CC, CG) Lunar

The term 'multiple SNP column' refers to the combination of three single SNPs selected, and 'allele genotype column' refers to the allele type at the SNP position of each single SNP in the same order. For example, in the multiple SNP No. 1 of Table 5, genotype of rs7761051 is AA, genotype of rs17517219 is AA, and genotype of rs6487998 is AG.

As used herein, the term "depressive constitution" means literacy, noise, sun and lunation, and the object of diagnosis provided by the present invention is preferably three constitutions of literacy, noise, and lunation, but is not limited thereto. This is because the constitution of the sun is very low in Koreans.

The present inventors went through a series of screening processes to develop a combination of single SNPs, ie multiple SNPs, from each of the single SNPs, which are highly related to each constitution of literacy, noise, and lupus. DNA is isolated and amplified from the blood of subjects corresponding to each constitutional group of cultivation, noise, and lull, and the sequence of the SNP position in the DNA is analyzed. Specific combinations of SNPs and their genotypes that were seldom seen in the outer population were identified. The SNP combinations and their alleles for each constitution identified in the Examples of the present invention are shown in Tables 4 and 5, respectively, and the characteristics of each of the multiple SNPs are shown in Table 6 below.

number Diagnosis Frequency of occurrence Ozbee 95% confidence interval Significance probability Degrees of freedom Diagnosis Constitution Other constitution One noise 10 0 75.06 4.34 1299.54 5.20E-09 24 2 noise 13 0 114.8 6.72 1959.91 4.59E-08 22 3 noise 10 0 75.26 4.35 1303.15 8.99E-08 24 4 noise 11 0 83.43 4.85 1435.47 1.05E-07 23 5 noise 11 0 83.49 4.85 1436.27 1.63E-07 23 6 noise 10 0 75.2 4.34 1302.26 1.65E-07 26 7 noise 11 0 91.83 5.33 1581.61 1.95E-07 25 8 noise 11 0 82.43 4.79 1417.94 2.64E-07 23 9 noise 10 0 75.68 4.37 1310.67 3.20E-07 25 10 noise 10 0 75.2 4.34 1302.26 3.51E-07 26 11 noise 13 0 106.6 6.25 1818.56 3.86E-07 25 12 noise 11 0 81.4 4.73 1400.06 4.84E-07 24 13 noise 10 0 79.72 4.6 1381.03 6.09E-07 22 14 noise 10 0 74.13 4.28 1283.83 6.87E-07 26 15 noise 12 0 89.62 5.24 1533.75 1.01E-06 24 16 noise 11 0 85.11 4.95 1464.46 1.05E-06 26 17 noise 11 0 85.65 4.98 1474.09 1.41E-06 26 18 noise 11 0 93.5 5.43 1610.66 1.82E-06 25 19 noise 10 0 82.29 4.75 1425.8 2.35E-06 26 20 noise 10 0 82.29 4.75 1425.8 2.41E-06 26 21 noise 10 0 74 4.28 1280.61 2.57E-06 26 22 noise 10 0 74 4.28 1280.61 2.88E-06 26 23 noise 10 0 82.29 4.75 1425.8 2.91E-06 26 24 noise 10 0 74.68 4.32 1292.59 4.06E-06 26 25 noise 9 0 77.26 4.42 1349.89 4.47E-06 26 26 noise 10 0 76.32 4.41 1321.33 5.35E-06 22 27 noise 12 0 89.32 5.22 1528.57 5.88E-06 25 28 noise 10 0 76.32 4.41 1321.33 5.96E-06 22 29 noise 12 0 89.32 5.22 1528.57 6.03E-06 25 30 noise 9 0 75.49 4.32 1318.55 6.31E-06 26 31 noise 11 0 78.66 4.57 1354.4 7.91E-06 25 32 noise 9 0 75.71 4.34 1322.03 9.02E-06 24 33 noise 10 0 82.29 4.75 1425.8 9.14E-06 26 34 noise 11 0 78.66 4.57 1354.4 9.45E-06 25 35 quality 12 0 57.6 3.37 983.65 2.52E-08 21 36 quality 12 0 57.6 3.37 983.65 2.52E-08 21 37 quality 13 0 61.93 3.64 1052.99 3.93E-08 26 38 quality 13 0 61.93 3.64 1052.99 3.93E-08 26 39 quality 13 0 61.45 3.61 1044.97 5.08E-08 26 40 quality 14 0 67.82 4 1148.88 1.19E-07 23 41 quality 13 0 62.4 3.67 1061 1.47E-07 22 42 quality 13 0 62.26 3.66 1058.66 1.79E-07 23 43 quality 13 0 62.5 3.68 1062.71 1.88E-07 23 44 quality 13 0 61.93 3.64 1052.99 3.21E-07 23 45 quality 16 0 78.46 4.66 1321.69 4.14E-07 25 46 quality 16 0 78.46 4.66 1321.69 4.14E-07 25 47 quality 16 0 77.85 4.62 1311.35 4.67E-07 25 48 quality 12 0 58.44 3.42 998.19 8.84E-07 22 49 quality 12 0 58.32 3.41 996.12 1.02E-06 23 50 quality 12 0 58.44 3.42 998.19 1.35E-06 23 51 quality 12 0 58.54 3.43 999.94 1.36E-06 23 52 quality 16 0 78.95 4.69 1329.61 1.45E-06 25 53 quality 16 0 78.95 4.69 1329.61 1.45E-06 25 54 quality 16 0 78.95 4.69 1329.61 1.45E-06 25 55 quality 16 0 78.95 4.69 1329.61 1.45E-06 25 56 quality 12 0 58 3.4 990.62 1.51E-06 23 57 quality 16 0 78.36 4.65 1319.56 1.53E-06 25 58 quality 16 0 78.36 4.65 1319.56 1.53E-06 25 59 quality 12 0 57.16 3.35 976.22 1.99E-06 23 60 quality 12 0 57.16 3.35 976.22 2.19E-06 23 61 quality 12 0 57.16 3.35 976.22 2.19E-06 23 62 quality 12 0 57.16 3.35 976.22 2.19E-06 23 63 quality 12 0 57.16 3.35 976.22 2.19E-06 23 64 quality 12 0 57.16 3.35 976.22 2.19E-06 23 65 quality 12 0 57.47 3.36 981.48 2.53E-06 25 66 quality 12 0 57.6 3.37 983.65 2.63E-06 26 67 quality 12 0 57.42 3.36 980.83 2.98E-06 25 68 quality 17 0 84.68 5.04 1422.56 3.30E-06 25 69 quality 17 0 84.68 5.04 1422.56 3.30E-06 25 70 quality 17 0 84.04 5 1411.81 3.89E-06 25 71 quality 19 0 96.46 5.77 1613.87 4.29E-06 23 72 quality 12 0 57.96 3.39 990.15 8.93E-06 26 73 Lunar 15 0 39.94 2.37 673.55 6.89E-07 26 74 Lunar 16 0 43.62 2.59 733.5 8.81E-07 23 75 Lunar 15 0 40.77 2.42 687.58 9.66E-07 25 76 Lunar 15 0 40.77 2.42 687.58 9.66E-07 25 77 Lunar 20 0 55.07 3.3 919.19 1.08E-06 26 78 Lunar 15 0 40.84 2.42 688.78 1.73E-06 25 79 Lunar 15 0 41.25 2.45 695.8 1.97E-06 25 80 Lunar 16 0 43.62 2.59 733.5 2.41E-06 23 81 Lunar 15 0 40.63 2.41 685.22 3.12E-06 25 82 Lunar 15 0 40.63 2.41 685.22 3.12E-06 25 83 Lunar 15 0 40.63 2.41 685.22 3.12E-06 25 84 Lunar 29 One 42.29 5.7 313.98 4.99E-06 24 85 Lunar 16 0 43.21 2.57 726.67 5.24E-06 25 86 Lunar 16 0 43.21 2.57 726.67 5.24E-06 25 87 Lunar 16 0 43.21 2.57 726.67 5.24E-06 25 88 Lunar 16 0 43.21 2.57 726.67 5.24E-06 25 89 Lunar 16 0 43.21 2.57 726.67 5.24E-06 25 90 Lunar 16 0 43.21 2.57 726.67 5.24E-06 25 91 Lunar 23 0 65.81 3.96 1093.45 5.30E-06 23 92 Lunar 17 0 46.58 2.78 781.53 5.80E-06 26 93 Lunar 14 0 40.55 2.4 686.38 6.36E-06 24 94 Lunar 14 0 40.55 2.4 686.38 6.36E-06 24 95 Lunar 14 0 40.55 2.4 686.38 6.36E-06 24 96 Lunar 16 0 43.62 2.59 733.5 7.48E-06 23 97 Lunar 17 0 46.58 2.78 781.53 8.05E-06 26 98 Lunar 16 0 43.21 2.57 726.67 8.50E-06 25 99 Lunar 16 0 43.21 2.57 726.67 8.50E-06 25 100 Lunar 16 0 43.21 2.57 726.67 8.50E-06 25 101 Lunar 14 0 39.92 2.36 675.59 8.56E-06 24 102 Lunar 14 0 39.92 2.36 675.59 8.56E-06 24 103 Lunar 14 0 39.92 2.36 675.59 8.56E-06 24 104 Lunar 14 0 39.92 2.36 675.59 8.56E-06 24 105 Lunar 14 0 39.92 2.36 675.59 8.56E-06 24 106 Lunar 17 0 46.64 2.78 782.4 9.25E-06 23 107 Lunar 16 0 43.56 2.59 732.62 9.60E-06 25 108 Lunar 15 0 40.63 2.41 685.22 9.73E-06 26

In Table 6, the term 'number' indicates the number of multiple SNPs and is the same as the number described in Table 4 above.

The term 'synagogue' is one of the noise, literacy and lume of the present invention among Sasang constitutions, and means the constitution to determine the constitution using multiple SNPs corresponding to the 'number'.

The term 'frequency of occurrence' refers to the number of constitutions and other constitutions of interest that have been found to have corresponding multiple SNPs within the 'constitution of interest' and 'other constitutions'.

The term 'odds ratio' refers to the ratio of the probability of having the corresponding multiple SNP among other constitution groups to the probability of having the corresponding multiple SNP in the constitution group of interest. That is, the odds ratio is ad / bc, where a represents the frequency of occurrence of the genotype noise group of the multiplex SNP of Table 6, and c represents the frequency of occurrence of other constitution groups, that is, the Soyang and Taein groups. Indicates. And b = [(number of total noise) -a] and d = [(number of total noise) -c]. The noise tested, Soyangin, and Taeinin groups were 91, 123, and 173, respectively, so b = [91-a] and d = [296-c].

If the odds ratio exceeds 1, it means that the multiple SNPs are associated with the diagnosis. The larger the odds ratio, the higher the association. As shown in Table 6, multiple SNPs 1 to 108 according to the present invention have an odds ratio of 39.9 to 114.8. Since this value is much larger than 1, it can be seen that the multi-SNP 1 to 34 according to the present invention is a noise constitution, the multiple SNP 35 to 72 is a small body constitution, the multiple SNP 73 to 108 is significantly associated with the Taeum constitution.

The term '95% confidence interval 'means a section having a 95% probability that the odds ratio exists, and is calculated as shown in Equation 2 below. If the confidence interval contains 1, that is, if the lower bound of the confidence interval is less than 1 and the upper bound is greater than or equal to 1, the association with each group cannot be considered significant.

Where V = 1 / a + 1 / b + 1 / c + 1 / d

The term 'significance probability' refers to the probability that the genotype frequency of each multiple SNP is obtained as the measurement result, calculated under the null hypothesis that the genotype distribution of each multiple SNP does not differ between the group of constitution and other groups of interest.

The term 'freedom' refers to the degree of freedom of the chi-square distribution used to calculate the 'significance probability'. In other words, the lower the probability of significance, the more likely the genotype frequency of the multiple SNPs obtained from the measurements is shown in the constitution group of interest and other constitution groups.

The multiple SNP for filamentous constitution diagnosis of the present invention may be composed of each of the multiple SNPs, more preferably, two or more multiple SNPs, and most preferably, all of the multiple SNPs 1 to 108 But it is not limited thereto.

The polynucleotide of each single SNP constituting the multiple SNP for filamentous constitution diagnostic diagnosis of the present invention is preferably 10 or more consecutive bases, more preferably 10 to 100 consecutive bases, but is not limited thereto.

The present invention also provides a microarray for filamentous constitution comprising a polynucleotide of the SNP, a complementary polynucleotide thereof, a polynucleotide hybridizing thereto, or a polypeptide encoded by the SNP.

The polynucleotide of the SNP, its complementary polynucleotide, and the polynucleotide hybridizing thereto are preferably 10 or more consecutive bases, more preferably 10 to 100 consecutive bases, but are not limited thereto.

The filamentous constitution diagnostic microarray is a polynucleotide capable of hybridizing to specifically distinguish a base of a polymorphic site in each polymorphic sequence of SEQ ID NOs: 1 to 124, a complementary polynucleotide thereof, or a polynucleotide hybridizing with them, By using the polypeptide encoded therein or the cDNA thereof as a probe, it can be prepared by conventional methods known to those skilled in the art.

Preferably, the polynucleotide is immobilized on a substrate coated with one active group selected from the group consisting of amino-silane, poly-L-lysine, and aldehyde. It is not limited to this.

The substrate is preferably, but not limited to, any one selected from the group consisting of silicon wafers, glass, quartz, metals and plastics.

As a method of immobilizing the polynucleotide on a substrate, it is preferable to use a micropipetting method using a piezoelectric method, a method using a pin type spotter, etc., but not limited thereto. It doesn't work.

The use and fabrication of the microarray has been widely provided in the art, and those skilled in the art having the information of the mutant sites that have been found to be used for the fabrication of probes, such as the sequences of the present invention, may readily manufacture and research microarrays. will be.

The present invention also provides a kit for filamentous constitution diagnosis comprising the microarray of the present invention.

The filamentous constitution diagnostic kit may be prepared by a conventional method known to those skilled in the art, and may further include a primer set used to separate and amplify DNA containing a corresponding SNP from a subject in addition to the microarray.

Such suitable primer sets can be readily designed by those skilled in the art with reference to the sequences of the present invention.

By using the multiple SNP of the present invention, it is possible to effectively diagnose an individual's filamentous constitution. Specifically, filamentous constitution can be diagnosed by separating the nucleic acid sample from the subject and determining the genotype of the polymorphic site, which is the base of each SNP position (101st of each sequence) of one or more polynucleotides selected from SEQ ID NOs: 1 to 124. .

The kit may further include a fluorescent material, wherein the fluorescent material is selected from the group consisting of a strepavidin-like phosphatease conjugate, a chemiflurorensce and a chemiluminescent material. Preferably, but not limited thereto, Cy3 was used in the preferred embodiment of the present invention.

The kit may additionally include a reaction reagent, which is a buffer used for hybridization, reverse transcriptase for synthesizing cDNA from RNA, cNTPs and rNTP (premixed or separated feed), chemical inducer of fluorescent dyes It may be composed of a labeling reagent, such as a washing buffer, but is not limited thereto, and may include all reaction reagents required for hybridization of DNA microarray chips known to those skilled in the art.

In addition, the present invention provides a method for diagnosing filamentous constitution using multiple SNPs of the present invention.

Specifically,

1) separating the nucleic acid sample from the subject;

2) labeling the nucleic acid sample with a fluorescent material;

3) hybridizing a nucleic acid sample labeled with a fluorescent material with the microarray of the present invention;

3) analyzing the reacted microarrays to determine the genotype of each polymorphic site of at least one polynucleotide selected from SEQ ID NOs: 1 to 124; And

4) provides a method for detecting multiple SNPs of the present invention for diagnosing filamentous constitution including the step of determining filamentous constitution using the genotype.

In the above method, the method of separating DNA from the subject can be carried out by conventional methods known in the art. For example, it can be done by directly purifying DNA from tissue or cells or by specifically amplifying and isolating specific regions using amplification methods such as PCR.

In this method, DNA includes not only DNA but also cDNA synthesized from mRNA. Methods for obtaining nucleic acids from a subject include PCR amplification, ligase chain reaction (LCR) (Wu and Wallace, Genomics 4, 560 (1989), Landegren et al., Science 241, 1077 (1988)), transcription amplification (Kwoh et al., Proc. Natl. Acad. Sci. USA 86, 1173 (1989)) and self-sustaining sequence replication (Guatelli et al., Proc. Natl. Acad. Sci. USA 87, 1874 (1990)) and sequences based on nucleic acids Amplification (NASBA) can be used.

In the above method, the fluorescent material is preferably selected from the group consisting of Cy3, Cy5, poly L-lysine-fluorescein isothiocyanate (FITC), rhodamine-B-isothiocyanate (RITC), and rhodamine (rhodamine). Not all phosphors known to those skilled in the art can be used.

In this method, the determination of the base sequence of the isolated DNA can be made by a variety of methods known in the art. For example, by using the dideoxy method to determine the nucleotide sequence of a nucleic acid or a probe comprising a sequence of the SNP position or a complementary probe to the DNA and by measuring the degree of hybridization resulting from the hybridization of the polymorphic site Methods for determining the nucleotide sequence may be used.

In the above method, the degree of hybridization may be achieved by labeling a target DNA with a detectable label to specifically detect only the hybridized target DNA, but other electric signal detection methods may be used. More preferably, the method may comprise hybridizing a nucleic acid sample isolated from the subject to a polynucleotide comprising a SNP according to the present invention or a complementary polynucleotide thereof, or a polynucleotide hybridizing thereto, and then detecting a hybridization result. have.

In the above method, the diagnosis of filamentous constitution is performed when the genotype of the polymorphic site of one or more polynucleotides selected from SEQ ID NOs: 1 to 124 is one or more of 1 to 108 in Table 5, and the subject is included in the diagnostic constitution group. Can be determined to belong to.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following Examples are only for illustrating the present invention and do not limit the present invention.

Example 1 Screening of Multiple SNPs of the Present Invention

We isolated DNA from the blood of Sasang Constitution Classifiers that were commonly determined to be noise, Soyang, Taein or Sun by three Sasang constitution experts among Koreans, and performed genotyping using SNP microarrays. In the combination consisting of three SNPs from the genotype data of the SNPs performed, statistically analyzing the combinations appearing specifically in the patient group, the multiple SNPs capable of diagnosing noise, soyangin, or lunium constitution except for Sunin among Sasang constitutions. Screened.

<1-1> DNA Sample Preparation

Genomic DNA extracted from the blood of 91 people, Saeyang 123, Taeeum 173, and Sun 3 were classified and classified by Sasang Constitution from the Constitutional Gene Bank of Korea Institute of Oriental Medicine. Chromosome DNA extraction was performed using known extraction methods (Molecular cloning: A Laboratory Manual, p392, Sambrook, Fritsch and Maniatis, 2nd edition, Cold Sping Harbor Press, 1989) and commercial kits (Gentra system, D-50K) (Gentra Inc., USA). Among the extracted DNA, the purity standard was selected and used only at least 1.7 in the UV measurement ratio (260/280 nm).

<1-2> Genotype Analysis of SNPs

Genotyping of SNPs was performed using a microarray (GeneChip Human Mapping 500K Array Set and 5.0 Set, and GeneChip Fluidics Station 450 system) (Affymetrix, USA) to which about 500,000 probes were immobilized.

<1-3> Screening of Multiple SNPs

On the basis of genotyping results using microarrays performed on 91 noise persons, 123 yangyang, and 173 taeumin analyzed in Example <1-2>, that is, a combination of SNPs frequently found in each constitution, ie Multiple SNPs were selected.

First, genotyping data for 440,974 SNPs for 387 were obtained from the genotyping using the microarray. In the present invention, the use of multiple SNP markers increases the discrimination ability of phenotypes rather than the use of a single SNP marker. In the present invention, it is intended to use multiple SNP markers in which three SNPs are combined. Since the number of SNP markers corresponds to 8.6 × 10 ¹⁶ , it was not easy to analyze the given computational resources and duration. Therefore, SNPs were selected by selecting SNPs that are statistically significantly associated with filamentous constitution through an association study of a single SNP, and the filamentous constitution with respect to the combination of multiple SNP markers in a reduced SNP population. A two-step process of in-depth analysis was performed. First, SNPs related to filamentous constitution were analyzed using plink (Shaun Purcell et al., Http://pngu.mgh.harvard.edu/~purcell/plink/ ). In the pretreatment step, the Hardy-Weinberg equilibrium test excluded 26,133 SNPs (significance ≤ 0.0001), excluded 8,202 SNPs containing more than 10% missing values, and the minor allele frequency (maf). Association analysis was performed on a total of 305,803 SNPs, excluding 125,743 SNPs with less than 5%. From the results, 873 noises, 796 cattles, and 805 lumens were selected for Sasang Constitution-related SNPs with a significance probability of less than 0.005, respectively. The number of multiple markers obtained from a combination of three SNPs from selected filamentous constitutional SNPs is about 1.1 × 10 ^{8 for the} noise group with the highest number of associated SNPs, which can be processed using general computational resources and processing periods. Judging by For multi-SNP markers obtained by combining three SNPs from Sasang Constitution-related SNPs selected by each constitution, each group of noise, small, or Taeumin group is used as the diagnosis group, and the other group is the other group. The odds ratio for the frequency of genotyping data and the 95% confidence intervals for the frequency of genotyping data were analyzed to evaluate the risk factors of the multiple markers and the chi-square test. Statistical significance was evaluated.

In this case, the odds ratio is represented by oz ratio = ad / bc when the number of constitutions and other constitutions of interest having or not having any genotype is as shown in [Table 7], respectively. Means deeply related to the diagnosis.

Numbers with specific multiple SNP genotypes Numbers that do not have a specific multiple SNP genotype Diagnosis group frequency a b Other constitution frequency c d

Ozbi and its 95% confidence in multiple SNPs of approximately 1.1 × 10 ⁸ noise, approximately 8.4 × 10 ⁷ , and approximately 8.7 × 10 ⁷ noises obtained from a combination of filamentous constitution-related SNPs selected for each diagnostic constitution Sections were calculated to select summers of 2.0 or more, and 108 multiple SNP markers with a significant probability less than 10 ⁻⁵ by chi-square test were finally selected. The 108 multiple SNPs are shown in [Table 4] to [Table 5].

Example 2 Fabrication of Multiple SNPs Fixed Microarray of the Present Invention

The present inventors prepared a microarray by fixing the multiple SNPs selected in Example 1 on a substrate. That is, polynucleotides composed of 20 consecutive bases including each SNP position (101st of each sequence) base in the polynucleotide consisting of each SEQ ID NO shown in Table 1 above (each SNP is 11 out of 20). In the second) was fixed on the substrate. Since two alleles exist at each SNP position, two polynucleotides for each sequence were immobilized on the substrate.

Specifically, the N-terminus of each polynucleotide consisting of 20 consecutive bases was substituted with an amine group, and then spotted on a silylated slide (Telechem), wherein the spotting buffer was 2 × SSC (saline-sodium). citrate, pH 7.0). After spotting, the mixture was placed in a dryer to induce binding, followed by washing with 0.2% SDS (sodium dodecyl sulfate) solution for 2 minutes and tertiary distilled water for 2 minutes to remove unbound oligos. The slides were treated at 95 ° C. for 2 minutes to induce denaturation, followed by 15 minutes with blocking solution [1.0 g NaBH ₄ , PBS (pH 7.4) 300 mL, EtOH 100 mL], 1 minute with 0.2% SDS solution, 3rd Each was washed with distilled water for 2 minutes and dried at room temperature to prepare a microarray.

Example 3 Sasang Constitution Diagnosis Using Microarrays with Multiple SNPs

We isolated the target DNA from the blood of the subject to diagnose filamentous constitution and fluorescently labeled it with Cy3-dUTP (Metabion). Under the UniHyb hybridization solution (TeleChem), hybridization of the microarray prepared in <Example 2> with the fluorescently labeled target DNA was performed at 42 ° C. for 4 hours. It was washed twice with 2 x SSC for 5 minutes at room temperature and then dried in air. After drying, the slides were scanned with ScanArray 5000 (GSI Lumonics) and the scan results were analyzed with QuantArray (GSI Lumonics) and ImaGene software (BioDiscover). As a result, it was confirmed whether the subject had all or part of the multiple SNPs according to the present invention, and the subject could be determined as one of noise, yangyang, and Taeinin.

Multiple SNPs, a microarray using the same, and a kit for diagnosing filamentous constitution using the same according to the present invention enable the rapid, objective, and accurate diagnosis of filamentous constitution using genome mutations, thereby increasing the probability of filamentous constitution diagnosis and genetic susceptibility. Accurate predictions can contribute to the development of alternative medicine-related industries.

<110> Korea Research Institute of Bioscience and Biotechnology <120> Multiple SNP markers, microarray and diagnosis method for Sasang          constitution <130> 9p-05-06 <160> 124 <170> KopatentIn 1.71 <210> 1 <211> 201 <212> DNA <213> Homo sapiens <400> 1 tggggatcct tacaggccac tcacaatggt aatgagagtc cacatttact gagcactcac 60 tacacaccag ccactcttct caagggcttt ccatgtggaa ytgcaccaaa tccttacaac 120 aaccctctga ggctggggct ggtgctgtat ccattctaca gaggaggaac ctgagacaag 180 gaagggtgag ggcttgccaa g 201 <210> 2 <211> 201 <212> DNA <213> Homo sapiens <400> 2 tggagccaac aaaccttccc tttagcatgg gcctagcgct cccttgccca ctgccagctt 60 tgttactctt agatccttaa tctctgttaa tctgatgact rtaaagcaaa aggttacttt 120 aatttgcatt ttcctggcaa ctggggagag gctgcatctt ttcaggtcat tgtgtgtctt 180 gtttcctctt ctgataattg c 201 <210> 3 <211> 201 <212> DNA <213> Homo sapiens <400> 3 ccaacttcaa attttgaacc tgtttaggga tctgttaatt aaagcagccc ctgcctccca 60 cacatccttc tcctctctgt ttttgttatc tactttcttc ratatggatt agccacttac 120 ttgaattaat ctgtttttca actttcaaaa gttagttaaa tttcccagta ttttggtgtg 180 cctactttgt tttttgacat t 201 <210> 4 <211> 201 <212> DNA <213> Homo sapiens <400> 4 ctggtctacg gtatttttgt tatagcagtc tgaactaacg aagacatatg gtactccata 60 ctggtgagaa aggttcttct cagcaagcct cattcttttt ygctttttga aaacctattt 120 ttttcttttt aaaaaaatcc catggcagca tattaatttt tttttctacc tggcagcaga 180 tcagattctt ttgccatggc t 201 <210> 5 <211> 201 <212> DNA <213> Homo sapiens <400> 5 ctttgcttgc aggaagcagt gcaaactgta ttttggaaca caggttcttt ctgacctggg 60 tccttatctt ctgccattta cctcttggaa gacttacgtt stactgacgc tggactactt 120 gacatttcct atccaagttg tgtctccagg cccttatcta tgctgctccc actgcctgga 180 ataccctttc cctctgccat g 201 <210> 6 <211> 201 <212> DNA <213> Homo sapiens <400> 6 actattgtcc cttcctgaag aaggaagtct agtcttcagg taagaggaag gtatattctt 60 aggcagctgc tgggttggaa ggaaggaaca gtgaagtctg ygttttggaa gggaggaggg 120 agtgacattt ttaagttaca agcctcttct gggagagctg gggacagatc tatttttaga 180 tgtgttccct gacacccagg a 201 <210> 7 <211> 201 <212> DNA <213> Homo sapiens <400> 7 tgcctctgcc aggccccacc tgctccactg cctctgttct gttgcagtga agtgctccgt 60 gtgcaagagc gtctcggaca cctacgaccc ctacttggac rtcgcgctgg agatccgggt 120 acagatctgt cctgcccttc acttttgcag taggagagcg aggccagagg gtgaaacaga 180 acccgcagaa cagtaaaggc a 201 <210> 8 <211> 201 <212> DNA <213> Homo sapiens <400> 8 ggactttatc aaagatggct gactagatgc agctgggaag tgctgtcctc acagagagaa 60 accaaaattt tgactacacc aacataattt gaacagatct ktgatgagaa aacaccaaat 120 gtggatgggg aaaagacaca gttgctgagt cggaagaggg ggaaagctgg gaatcccatg 180 tggagtgccc aaatgctgtg g 201 <210> 9 <211> 201 <212> DNA <213> Homo sapiens <400> 9 actatgctgt ttacaaagca ctttcatatg caggatctca tctgtattca gaggctcact 60 ggtgccctag gcagggatga tgttatcctg gagtaacggc rtaacttagt ctgtgaatga 120 cacaggacac attcttggca actctgtagg acagggcatt tggtccagac aggacactca 180 ccgggctaca aggtctagcc c 201 <210> 10 <211> 201 <212> DNA <213> Homo sapiens <400> 10 ccaatgtctg ctccaatatt ttgtaattga catgtatctg agtctaagag attaaaccaa 60 ctggcacacg acccaacagc atctttggtg attaaaagaa sattggttgt tgcctaaata 120 aaacctgggg ccacgttcgc ccccaggagg acccagtgct aatctcaaat cctgagaacc 180 agtcatttgg gctgggcaaa t 201 <210> 11 <211> 201 <212> DNA <213> Homo sapiens <400> 11 caaattcttc tagagataaa tatttttagt gtcttgaaaa tatattgcca aattttcccc 60 taaaacatgt ataagaatgc tcattttacc atacaatcac ygggcttgaa tatccatttt 120 aagtttaacc agccttaatg attccgttgt actttttttt tttttttttt tgagacgggg 180 cctcactctg tcgcctaggc t 201 <210> 12 <211> 201 <212> DNA <213> Homo sapiens <400> 12 tccaagggga aaaactatct taaaaaataa ttttctatgt attataaaga aagctcgggc 60 ctgttcaacc caacaattct gagttttcaa gtcattctat ragcaggtca accagaaatt 120 gcctgaaccc cctcccatag ccaaaaaact attcacccaa ctggcagaaa gcataaccag 180 cagcttgccc atctcttcat g 201 <210> 13 <211> 201 <212> DNA <213> Homo sapiens <400> 13 agggagaatc tacaccagga aaccataggc tcctgaggtt gaatgcaagt tgcgtgtgtt 60 cctatgtgca aagagaagcc atagctatca ttttctgagg ygtccaagac ccacaaaaag 120 ccaaagactg cactacccga gagactgcag caggaatgag tggattaagc cattcagcat 180 gtccagacat acaaaatcac t 201 <210> 14 <211> 201 <212> DNA <213> Homo sapiens <400> 14 catcgtgagc aatgtattta aggcctccca aaacaaaata atattagcat gacgtatatt 60 tggttaagtg tcataagtat cttaaagcac catccctttc maatctcctt tacagaacat 120 cactaagctg acctctgtat tacaaggcta agaataataa gttttaaaga gtgacaattt 180 tagaattttt cacaatggta t 201 <210> 15 <211> 201 <212> DNA <213> Homo sapiens <400> 15 tgccttttat aagttatgga acattaacat gaaatacata tagaatggaa tttggtagct 60 tgcattacat aaatttattt tggcgagtga agcccatagt yctcaaatcc gattcataaa 120 attaaaacct cttattttct tctggagcct ttaagggctt ttgaaaatca cagttaaaag 180 gtccagattc ttatcgttca t 201 <210> 16 <211> 201 <212> DNA <213> Homo sapiens <400> 16 tttcgaaggg aaaatgattt tatagaaagg gatgggtaac ctttatgata aaattgcttt 60 gctagagcgt gttgcttgtg acagcaccac ctttctgttc ytgacaatgt ttgtcttctt 120 aactaaacaa acgttagctg agttgaatga aattatgtac gaaccagaga agtcgggagg 180 atgtttgagg ctcgagcccc c 201 <210> 17 <211> 201 <212> DNA <213> Homo sapiens <400> 17 cctgtttgta gcccagctac aaacagaaga accaccaagc tgacttgtaa actataacat 60 gtgcttcttc atttaaagct tatttaaagt tgttgaactt ygggttggtt tggttcacag 120 aaatagctaa ccaataaaaa tgaataaaaa attctggtat gttcagaaaa tgaaattcaa 180 agatgaaaat gaacacatta t 201 <210> 18 <211> 201 <212> DNA <213> Homo sapiens <400> 18 gaatatggaa aataaaacca cttttggggt tggggtttcc tcaagccatt cttatttact 60 gagtcggttg cctctggaaa cttttagctc atgtaactaa rcatcgttca ctgccctgtt 120 ctttatgcat ctcagtaagt tccacctgca gaatggtgaa attttgaagt ggaaaggacc 180 ttgatcataa attagttcaa t 201 <210> 19 <211> 201 <212> DNA <213> Homo sapiens <400> 19 tatttaagtc atttgcactt gtgtgtgttt gtaagaactt acgtataaaa tgttcctagt 60 agattttaaa aatgatatta ttttaatatg aaattggcta rtgaagttct agagaattaa 120 tgtttacatt ttgacaacca aattccttat gtactttaca tgtgtttact taaaagtctt 180 acaaaataga atttgccctt c 201 <210> 20 <211> 201 <212> DNA <213> Homo sapiens <400> 20 tgataatgtt ctggcaggtg gcagaaaagc atattgcaga agaggtattt tttctgattt 60 gcacaaaggc atcatattgg atagatgcaa ttgtgagttc yttaactgtt tctttttccc 120 attccttttg cgatacctct ctacctttac tcttctagta tttcctggct atgaaattgg 180 ctaagggcag tggtggcaga a 201 <210> 21 <211> 201 <212> DNA <213> Homo sapiens <400> 21 taactgattt tctacttagc atgctgtttt taaggttcat ctatgttcta gcatgtatca 60 atacttcatt ccttttatgg ctgaataatg tttcattgtc yggctatact acattttatt 120 tatctattta tcagttgata gatatctggg ttgtctccac cttttagcaa ttacaaatag 180 tgctgctata aatatttgtg t 201 <210> 22 <211> 201 <212> DNA <213> Homo sapiens <400> 22 gaaattgatt aaatatggct cttttcctaa gcagatgaat aactctatat tgctcgttct 60 gcatgcctat aaaataatac aatgaacaat gttgacttca kaattgagaa catactccca 120 ttttgctagg tataggcaaa agagaaaaca caagctaaaa cctcaatatc aaggtaatgt 180 ttatagcact atattttcac a 201 <210> 23 <211> 201 <212> DNA <213> Homo sapiens <400> 23 ccttacctgg gagccatcta ctctggaaac catggatacc tacacatctg tgcaaaattg 60 acacagtgcc tcagttattt gggtaaggct gaccagtgaa ytctgataaa ttgctgtaac 120 aatgaccacc catctgtcaa tgtgtcacca agcataggct gtatacactg tgcagactgt 180 aaggatccac tgggctttcg g 201 <210> 24 <211> 201 <212> DNA <213> Homo sapiens <400> 24 tttaaaaaaa actttctgtg aggttcttgg agaagaacct atgaatgagt atgaattccc 60 cttgtgtctg tgacccccag ggtttttaga gactcttgtt ygcttataat aaacctttag 120 caattagtta aaaaatttag cttacttttt cttaccattg tccagtagta tctgtttcag 180 gtaagcaagt gcttgtgact c 201 <210> 25 <211> 201 <212> DNA <213> Homo sapiens <400> 25 gaccttttga catatagggc ctagctgtaa tacatttcaa tgttcagtct ccatcccaag 60 gtgaacatgg gtcatatgtt atatacatat tgtttcaata ygcccgtgtc aggactacct 120 tcatgagtat acatagcact tcctgcaacc tattgaatat gtatgtttag ccagccgggt 180 tggcataaag ctcctacccc a 201 <210> 26 <211> 201 <212> DNA <213> Homo sapiens <400> 26 atagatcaaa atagcctggt ttgttacttc ctcaggtagc tagattatgt ctggattcca 60 gattttgttg tctgaaaagt aagcttctca ttgcttaagt stctcttata ggtttctctc 120 atatgtagcc aagtacaatt catgatgata tagtaaataa aacacatgat gttgtcctca 180 gcaaacctac agttttacaa a 201 <210> 27 <211> 201 <212> DNA <213> Homo sapiens <400> 27 ccgcagcccg gtggctcaga cctgcgaagg aaccaaggca agagggagaa aagccctgcc 60 gcctgatccc acgccgccac tcacagaccc ttcgttgacc sgcagcattg aacaggaaaa 120 aaaaaaaaag atgaaaacac agaaaacccc aaaaacccag acggggagac gatgtgggga 180 agagaacgtg ctgggagcct c 201 <210> 28 <211> 201 <212> DNA <213> Homo sapiens <400> 28 tatgattaca ggaggtacct ggagtagtca aatttataga catagaaagg aggatggtgg 60 ttgccagaat gggtagaatg gggagtggtt atttttcatt yttctgagat gaggtctcgc 120 tctgtcaccc agggtggagt atggtggtgt gatcatagct cactgcaact tcaaattcct 180 ggactcaagc aatcctccca c 201 <210> 29 <211> 201 <212> DNA <213> Homo sapiens <400> 29 gtgtacttca gtgggttttt gtagtggctg gtaatgatct ttccctatca tatttagtgc 60 accttttagg acctcctgta aggcaggtct ggtgataatg mattttctca gcatttgctt 120 gtctgaaaag gatttttatt tctctctcac ttatgaagat tggtttggct ggatatgaaa 180 ttcttggttg aaaattattt t 201 <210> 30 <211> 201 <212> DNA <213> Homo sapiens <400> 30 acctttccct tctgttaaca cggcggtaat ttattacata agagatgtta atcctgggga 60 gaaattctaa atactcaggg aataagtaat ggctgggccg yaaatgatga aaatgtgaat 120 gtgttacata gattctgaat tattcctggc attccctggg gagagagaat agaagtaagt 180 tgcaatcagt tgtgatacct c 201 <210> 31 <211> 201 <212> DNA <213> Homo sapiens <400> 31 ggctgccgtc aaaagtgcat ggacgagtaa agcctggcat caccagggaa tgaaaaaccg 60 cacagtgaat gacctcggcc tggcagtccc acacttaaaa mtcgattgtg aaggcatgct 120 gagaaaggca ccagatgatt tgtgtccaga cctgttcgct ggacggagtg aaaaggtgtg 180 caacacataa agggccggcg g 201 <210> 32 <211> 201 <212> DNA <213> Homo sapiens <400> 32 atgaaatcat tttaggctgc tattggtcat ctataatatt tttaaaatct gccaaagatg 60 tcaccaaaga aggttccatt gtatattatg tgtaacatga rggaattaaa caaataaaat 120 gaataaagtg tttatacatg aaaaaatatc aaaagtgtgc atactggata cctgacttcc 180 atcccaagaa taatttcctt c 201 <210> 33 <211> 201 <212> DNA <213> Homo sapiens <400> 33 aataattagt gttcataaca tttggaattt cagtatgttt aaattcatat tatgagaaga 60 tcaacaggct tattatgttt gcataaatat tagtataggc ygcttttatt atagaataaa 120 gaagaataaa cattcttttg tcttttcaaa atacagctat gcacatacac atgaacacaa 180 aactatcatt tacaaattat t 201 <210> 34 <211> 201 <212> DNA <213> Homo sapiens <400> 34 agttcctgcc ctccagttgc tcacagtcta gcaagggatg gaggagacta attaagaaac 60 cttttattac agcacagctg actgcacgaa ccaaaggaga mgcatgagcc agagagacaa 120 ggaaggcttt caaaggaagg gatggtcaag ctgggtttcc aaggatgact aggagctggc 180 caggcagcaa agggcattcc a 201 <210> 35 <211> 201 <212> DNA <213> Homo sapiens <400> 35 acctcctgct gtgtggcccc gttcctaaca ggccacggac cagtaacagt ccaaggccca 60 ggggttggga atcctgcctt aaggcgcaga gcagttacat yatggcactg aagtttatac 120 atcagtttct tgaaagtttc attatcatgt gtctttcccc aggactgacc agctctcagc 180 acaacttttt aagaaaatga a 201 <210> 36 <211> 201 <212> DNA <213> Homo sapiens <400> 36 aatggctaaa tccatgtcaa aatgtgcaga ataatataat cttatactgt ttcacctatc 60 aaattagcaa ataattttaa aagttctatt cctcatagcc rgtgtgcata tgctgagaga 120 agtccttttt tttcctgtgg gaagcttaat tgataggaaa ataattaatt gaaaaataat 180 ttgacagtat gtaccaattt t 201 <210> 37 <211> 201 <212> DNA <213> Homo sapiens <400> 37 ttccaatttc tagacttttc atgaaatatg ggaagggtgt aaccagaagg actactacat 60 ggtaccttta ggccttgact gtcagtaccc ctccatcagc rtgagtcttg ggcagagctg 120 ggcatcagtg aacagacaca gtgaccaggt acctggcctt cctcacccct tgctgccctc 180 tggccctcaa agccttgagc c 201 <210> 38 <211> 201 <212> DNA <213> Homo sapiens <400> 38 tggaacacag gttctttctg acctgggtcc ttatcttctg ccatttacct cttggaagac 60 ttacgttgta ctgacgctgg actacttgac atttcctatc saagttgtgt ctccaggccc 120 ttatctatgc tgctcccact gcctggaata ccctttccct ctgccatgcc caccaggtga 180 atcacagctt atcctttaag a 201 <210> 39 <211> 201 <212> DNA <213> Homo sapiens <400> 39 ctccacttac tggctattct ttggtttctc tcctctcctt gagccttcct atgccctcag 60 atacaacagt attgaaattt gatcaattag taatcctata rtggtctcta agtgttcaac 120 tgaaaggaaa agttgcgcat ctttctttct ttaccgagac agggtctcgc tctgttgccc 180 aggctagaat gcagtggcgc a 201 <210> 40 <211> 201 <212> DNA <213> Homo sapiens <400> 40 tattttgcat gtaagaagga caaaaatttg ggggtgctgg gcagaatgtt aatgagtgaa 60 tatgttctag aaaaagcgca ttgaaggaga gaaaaatgtg ygagaaagag accaatccat 120 ggtgaaaagt cagtggttta cactccagag aagaactaat cagctctgtt tttatatagc 180 ataagtttaa catgctggga a 201 <210> 41 <211> 201 <212> DNA <213> Homo sapiens <400> 41 caccgcacct ggccccctag caggttcttg atagttgctg acaatggagc tagctgggga 60 ttctttcctc cgatggtcat tatgcattat agacaaggtt yctgtgttct cattatgccc 120 ccaaggaaca tgcttcagca aacgtgaaaa atgccagagc catacatttt tttttttttt 180 tttttgagac agggtctccc t 201 <210> 42 <211> 201 <212> DNA <213> Homo sapiens <400> 42 ggtcacaatt gtgcctctgt gcaggcatgc ttgcgcccct agccttctag tgtttatcct 60 tgctgatctt ttgatttctc tcacagaacc ttggatacct ygtttcccat ttcctggtga 120 ggtcaccacc ctctccggtg gtggcgctgg gccctgatct ttgctgtttc tccaccccat 180 gtgttctgtt tcgcttccat t 201 <210> 43 <211> 201 <212> DNA <213> Homo sapiens <400> 43 agacggggtt tctccatgtt ggtccggctt gtctcaaact cccgacctca ggtgatccac 60 ctgccttggc ctcccaataa catccctact ttaggctaca ycatatataa tgagtttagt 120 tggttaatgg ctacccaaat tcaagtagta agcaaagtac ctttatgcct tgagaggcat 180 gaaaacagca gcctccaggc t 201 <210> 44 <211> 201 <212> DNA <213> Homo sapiens <400> 44 gttcccaagc aggaagctgg ggcgtgatta aatggatgcc ggacaaccaa aaaagcaaaa 60 gcccgcttca cagccacatc cagtgtctaa atccacatca yctaaaagta cctggaagat 120 cataggcttc ctcgtcaggt cctccaaggc cacttccatg atcctgcttc taggactggg 180 accccacccc cttcacacgg c 201 <210> 45 <211> 201 <212> DNA <213> Homo sapiens <400> 45 tagaatggga gacagatttt ccctgagtgg ttcccagctt gaaagggcct aagatatttt 60 cctttcacaa gaggtatgcc cgtaatacct tgaagaactt ygtgtaacaa ctaaataaat 120 aaatggtaac aaattagcac tcaataaatg tcagctatta gcttttccaa ataatcctaa 180 aatgtgcaac ctcttgttta c 201 <210> 46 <211> 201 <212> DNA <213> Homo sapiens <400> 46 tggtcctcat tctgcacctt gctgttctgt tctatgtaac atccaggctg ggaggggagg 60 gggccggccc tcctcctctg tcccaggctc tgcagtgggc racttggttc cgtccttacc 120 tcctcagctg ggcatgtgat cttggtgctc tgagccttgt tctctgtgct aagtatatgg 180 ggttcattgg aaaaactgac a 201 <210> 47 <211> 201 <212> DNA <213> Homo sapiens <400> 47 aaggatgaga tcaggtatgg tcagcatccc tcacctccca aatcctcttc caagtcattc 60 ctcttgcacc cttgtgtgaa aacccttcct cctttgagag rctatcaatc tggctctatc 120 acagtgtcag tcaaaattcc cttggatgta aacaacagaa actggctggc tgatgtagcc 180 tgaaaatgaa tgtattcaaa a 201 <210> 48 <211> 201 <212> DNA <213> Homo sapiens <400> 48 caggcccatt ttgcagaggg gaagactgag gtgcagtgat gcaaaatgtg tggtggaggg 60 ctgggcctgg aatctgggag ttctggttcc gggtgaccgt mcactagaaa gggaaaggtg 120 gcagaaccat atatatgagc ctgttacttg ttcaaggact tggtcagggg cctcacagaa 180 cttggaaaag agcaacggac t 201 <210> 49 <211> 201 <212> DNA <213> Homo sapiens <400> 49 catgtgttcc ttatagttca tccatccaaa aaaattgcag ttcctcaatg ttacactctc 60 ttggtctgtt ctcttagtgt aatcttcccc atacacattc yttgaccaaa attaaccttt 120 aatacttagc tcactttggg aggccaaggc aggtggatca cttgaggtca ggagttagag 180 accagcctgg ccaatatgat g 201 <210> 50 <211> 201 <212> DNA <213> Homo sapiens <400> 50 ccactcccca agccaccagg tggccctgcc cacacctgga ttcatttaac cccatgagac 60 cgtgtcggac tcctgccctc cggaattgcc agctaataca kttgtgttgt tcaaaccact 120 gaagttcatg gtcagatgtt atgacagcag aaatagcctg atacactgtg agaaagtgga 180 gagcacacga cgcctcggga a 201 <210> 51 <211> 201 <212> DNA <213> Homo sapiens <400> 51 gggagctagt taggcaatta gtacagtcgt tgagaaaaca ggtaatggta gcttaaacta 60 acaggaggat aaaggagaag agaagtacat gggttttcaa yacatctaag aggtaagcta 120 gcagggtttg gctatcaatt gggcatgaaa agtaagggag gggaatgtat cataagagta 180 acgtctcggt tttagagtag a 201 <210> 52 <211> 201 <212> DNA <213> Homo sapiens <400> 52 caagtgaatc actaatgaca tttgttggtt tattggttga aaagtacttg ctgtagataa 60 actatccacc aggccatgtg ctcttcttca cctcagaacc rtgccttagt ttatcactag 120 ctgcctcctt ctctttcttt atatcctagc ttcaatggca gtctttcctc atcactttct 180 ctaattaaac cacctattct a 201 <210> 53 <211> 201 <212> DNA <213> Homo sapiens <400> 53 acaagtctct aaatgaggta cgtaagttat tactccagac atcagggagt attttgttta 60 tgaaacatct ccaatttatc actagctggt tatcattatt ytcctcataa atctatagat 120 gctggtttta ttttctataa gccatcctta gccaaggaca tcccattatg acctttccct 180 agataaggac tccattccta g 201 <210> 54 <211> 201 <212> DNA <213> Homo sapiens <400> 54 tataattttt tagaaatagc tgtgacatga aatatggcct tgacagagaa tgaagaacat 60 atcaagaaca acaaatgacc aacatgaatt gtctctgata ygctatttca tttacctgtt 120 tatgaaatgg aatatttcta gtgctcttta tcatcaaatg caaatggaat atgtgaggag 180 gcctggcagt tccaccagga t 201 <210> 55 <211> 201 <212> DNA <213> Homo sapiens <400> 55 agaatcttga agatttacct gttcccatag gtactgcagc attattcata atcattaaga 60 tatggaaaca acttagatgt ccattgacag atgaacgaat raagacaatg tgatatctat 120 ctatctatct atctatctat ctatctatct atctatcatc tatctatcta tatcatctat 180 tatctatcta tctatcatct a 201 <210> 56 <211> 201 <212> DNA <213> Homo sapiens <400> 56 aggtctttac aatttggcat gtttttgcag tggctggtac cagttgttcc tttccatgtt 60 tagtgcttcc ttcaggagct cttgtaaggc aggccttgtc rtgaaaaata tctctcagca 120 ttttcttgtc tgtgaaggat tttatttctc cttcacttat gaagcttagt ttgtctggat 180 atgaatttct gtgttgaaaa t 201 <210> 57 <211> 201 <212> DNA <213> Homo sapiens <400> 57 catgagaccg tgtcggactc ctgccctccg gaattgccag ctaatacagt tgtgttgttc 60 aaaccactga agttcatggt cagatgttat gacagcagaa rtagcctgat acactgtgag 120 aaagtggaga gcacacgacg cctcgggaaa acacctcctt ggctgggttt tgctaaagca 180 aaaatagaaa tccaatttag t 201 <210> 58 <211> 201 <212> DNA <213> Homo sapiens <400> 58 ttcctggagg tgaactctta ctcatgctat agaactcttc ccccaattcc cccgctctgt 60 gagttatccc atcataagac agacgcaatc tcaaatttct stattcattc tccaacagtt 120 atgacaacgg ccagcattca ttgagcatgt actaattgcc agacactatt ctaagctctt 180 tacacttttc tttctctctc t 201 <210> 59 <211> 201 <212> DNA <213> Homo sapiens <400> 59 accaaggttt ccaaagctgt tgaataaatg aaaggtaaat aaaaatgcat tgaaccccaa 60 aaaagctgct gaagggaatt ttcataatgg aagggaagta ytttttgctg aagggaatct 120 ttacaaaagg aagcattttt gatgcttgtt tttaagagat atacgtatat atacattaat 180 aaatacacat atttacgtta a 201 <210> 60 <211> 201 <212> DNA <213> Homo sapiens <400> 60 tgctcttctc gaggagtatc tttgtggtgt tctctgtatt tcctgaatct gaatattggc 60 ctgccttgct aggttgataa aattctcctg gatgatatct kgcagagcgt tttccaactt 120 gtttccattc ttcccatcac tttcagctac accaatcaaa catagatttg gtcttttcac 180 atagtcccat atttattgga g 201 <210> 61 <211> 201 <212> DNA <213> Homo sapiens <400> 61 taaaaaatag gagaaaatct ttttgacctt gtattaggct aaagattatt agatatgatg 60 ccaaaagaat aatctataaa agaacaaatg gataaattgg wcttcataca cataaaaaac 120 cttcagctcc ccaaatgata ctgttaaaaa aaatgaaaag acaactacag gctggaagaa 180 aatacttata aagtgcatgt c 201 <210> 62 <211> 201 <212> DNA <213> Homo sapiens <400> 62 tttttttgtg ttcttgtttt ttggagcatt aaaagacagc acttcctttc tactatatga 60 caacccttca aatcaatgaa agagctgtta tgattgtcct rctatgatcc taagttaaac 120 ccatgagttt tcctcatctg ctcaacatgt ggtattgcct tcagaatctt cactagtctg 180 gtcactctac tgttgataga a 201 <210> 63 <211> 201 <212> DNA <213> Homo sapiens <400> 63 cccatgagtt ttcctcatct gctcaacatg tggtattgcc ttcagaatct tcactagtct 60 ggtcactcta ctgttgatag aatcaacctg ttcgtgattg ycctaaaatg tgattaccca 120 gggagggatc acttagtttg ggctgcagtg ggactatgaa tcctgtgttg aaattacaaa 180 ctacttttat tggcctggcc t 201 <210> 64 <211> 201 <212> DNA <213> Homo sapiens <400> 64 ccctcacctg ccgtgcctga cacccgccag caacacacac ttgggaccag tcctgctgcc 60 ctgtcacccg gcatggctga ccctgaccgg ggccttttcc ragctgctgt agctcatctc 120 agcccaatgc cactgggaga cattgcagca catctctcac cttcgactgt ggaatatttg 180 aggacaggcc tgtgactcac c 201 <210> 65 <211> 201 <212> DNA <213> Homo sapiens <400> 65 ttcatcatac ccacccagat ttttagccct gtgtgtgcat acctgttgga aacaaagacc 60 acatttccca gctcccatgg agctagatgt tgcaatgtga mtaagttttg ctcaacgaag 120 catgagcaga ggtactgggt gacttctagg aagtgtcctt taagggtggg gcacaacctt 180 ctgcccttcc tccttcttat a 201 <210> 66 <211> 201 <212> DNA <213> Homo sapiens <400> 66 taatatgagc caagtttttg ttccctgtgt caggtactga cacagtggca ctcagtgctc 60 tactacagtt ggcttatttt taatcatttc aattatcctc rggtagaaca tcatccttga 120 ctgagagaac aggaaactga ggacaacaga cgtttgaaaa tgtgttaatg tttcacagat 180 aatggcagag gacccctgga t 201 <210> 67 <211> 201 <212> DNA <213> Homo sapiens <400> 67 ggacataata ttagacatag tcatagaaat ccttaataaa atattagcag atagaattaa 60 tcaatatatg aaaagtgtat ggtgtataca tgatcaaggg rgttttattc caccgatgct 120 tgatatttga gaatcaatgt acaatcatga catagtaaac ataacccacc acagtaacag 180 gctaaaggaa ggtaatcata t 201 <210> 68 <211> 201 <212> DNA <213> Homo sapiens <400> 68 gcctcaaagc tcctgcaccc ccagcacttg ggctgaccaa agagaacagc agggactttt 60 cttgtgtgtc acccaaggaa gaaggaagtg acaaacaatg rccaaagata ggggtggaga 120 ccttaaatgc ccacaggggc caacaggtat tgtaaccaag tggcaaggcc agctgaagac 180 actagggagg ctgaagacag g 201 <210> 69 <211> 201 <212> DNA <213> Homo sapiens <400> 69 agttattcat tctgtataac tgaaattttg tactctttga ccaacatctc ctcatctctc 60 cctccccaca gtccctggca atcaccattc tactttctga ycttatgagt ttgacttttt 120 tttagattcc tcatgtaact gagatcacgt ggtctttacc ttcagtgtgg agaaaaggga 180 acctttttac atggttggtg g 201 <210> 70 <211> 201 <212> DNA <213> Homo sapiens <400> 70 tttataaatt atccagtctc agggaaattc tttatagcag tgtgagaatg gactaatata 60 aggggcaaag agaatcattc ctggacagct ctgtagactg ytgcaagaac tattccagtt 120 gaagacaaat gtgtgactct gccctgctgg tgagctttgt tttctctcct atattcatcc 180 aatgaatctg acgtctaggt t 201 <210> 71 <211> 201 <212> DNA <213> Homo sapiens <400> 71 tgggctttcc tccaagtgtg aaaccctgga tgcgttctga aatccccaag gatgctgaga 60 gccacagacg tgtagacgga aagaaatcaa accattcgcg ytgctgatgg tgggaggaag 120 tgcgctgcag cccgcacctg cctcccctga tgccaacagc aggaccggca ccctcgttct 180 ctgcagtcac actccccatg t 201 <210> 72 <211> 201 <212> DNA <213> Homo sapiens <400> 72 tggaccagaa aaagaaaatg aggtgacaga caaatgtatc atctattttc agtgaagagt 60 tgctccctcc aggaaagcca tttgtgatta tttaattgcc rtgaaatttt cctcaggttt 120 aataactagc atgcagggaa aatcatttat tacatgaata cttgcaataa tcaaaatata 180 tttagaagat tttaataatt t 201 <210> 73 <211> 201 <212> DNA <213> Homo sapiens <400> 73 ataatgtttc catggaaaag tctgcttcca ggcttattga ggctgcattg catgttattt 60 ctttcttttc tcttgtgctt taggatcctt tctttatcct ygacctttgg aagttagtga 120 ctgttagttg ccttgaggta gtcttctttg ggttaaatct gcttggtgtt ctataacctt 180 cttggagtta actgttaaca t 201 <210> 74 <211> 201 <212> DNA <213> Homo sapiens <400> 74 ccccgagtcc ctactattac accaggtctc ccctgcctag agaagtgagc cttacagctg 60 aatgcacatt tcatagaaac caagaggtta ctcttcattg ycgaagtgaa gcattagtct 120 tgtaaaatta aaaaaaaaaa aaacaggcca tctttgagtg aataatgaga tacttgcctt 180 aatttgattc ttaattataa t 201 <210> 75 <211> 201 <212> DNA <213> Homo sapiens <400> 75 acatttgggt tttgtttcca tatggtggtt attgtgaata acgctgcaat gaacatgtga 60 gtgcagatat atctacaagt actactttca tttcctatgg ktgtatatcc agaagaggga 120 ttgctgaccc acatggtagt tctattttta atttttcaat gagcctcatg ctctgtcccc 180 taatggctgt tccaatttac a 201 <210> 76 <211> 201 <212> DNA <213> Homo sapiens <400> 76 catttaagtg gaagaggata atgtgtcatt gatcttgagg tagttggtaa aagtgagaga 60 gaaaaataca ccgtaggaaa accaaacaaa acaaaactgg rccatttagc ctggcaggga 120 cagaaataag gagaccctta agagaggagt ttgaaatcac agtaaaaagt gcaagtgatg 180 tgatacaata gaaaacacgt t 201 <210> 77 <211> 201 <212> DNA <213> Homo sapiens <400> 77 gggagagcta gccttaaaat gtaaccgaga ttaagaaagg cagaggaaaa acaaaagaaa 60 aaaatataaa caaaagaaag aaatgacaac ctcattgtaa yggtaaatca agtcaaaatg 120 gaagccagag gcaccagtgg gcttttaaaa ttacgtaagc taataatttc cctttattgt 180 taagccagtt tgagttggct t 201 <210> 78 <211> 201 <212> DNA <213> Homo sapiens <400> 78 ccaaggacag agcactgaga ctgtaaagga ctaggagaca ctgatcacta tggggagggg 60 atatccctgc atgcaggaca acaggaccca ggctagatac rggtaaatgc agatttcctc 120 tgctactgac actgtttaga taccgtgaga cattctgagt atgaagttga agctagtagc 180 aaactactca aagattaaat a 201 <210> 79 <211> 201 <212> DNA <213> Homo sapiens <400> 79 aaaaacctat gagaatattt tgcagtgaga gtttagtttc atttttgttt actttgcttt 60 tgcatcaata atatcttcaa gagaagaaac attagacaga kttttagtag ttaggtcaac 120 tcctatcagt ggttttcata cttttctttt tttccggatt cctttgtgaa agtaaatttt 180 ctgatgttga attgttttca g 201 <210> 80 <211> 201 <212> DNA <213> Homo sapiens <400> 80 gcagtccaca gtgtttacag agtgggacta tatacagtca cccttctctc acccgtcctc 60 cctgctctga gacacacccc tgtggcgagc accgcagaaa ygggaagcca ctacttaaga 120 tcggaagtta agagagctcc ctccgaagag aaaattttta tactaaaatc tataatttaa 180 ttcaagagaa tgcttttatt t 201 <210> 81 <211> 201 <212> DNA <213> Homo sapiens <400> 81 aagccaccat ccctggccaa ccgtacatac ttttttaaat gtatcagagg aaagaaacag 60 gacacaggtt ggggatagaa tctagctttg tctgaatgta mcttgttttg gaaccatgta 120 aatattttac atacttagta aaatacattt aaattttgaa aatgtaccct ctaaaaactg 180 aaagcagagc aaatgtattg a 201 <210> 82 <211> 201 <212> DNA <213> Homo sapiens <400> 82 ccactttccc tttgcttttg aggaacattt cagaaactat gttcaacaaa tactactgaa 60 tttgtattga attggccaag acaataattg aaaccagaaa kaaagttttt cattgggaaa 120 tggtttctac taacaaattt agtatgtcca cattaaaaaa ggtgagagat tatatcacct 180 tctctagatt gctacttttc t 201 <210> 83 <211> 201 <212> DNA <213> Homo sapiens <400> 83 gggtggactt tggggagtct gcatgctctc cagctagtgt tgcctgtggg gagcaggttt 60 gcaaggtttg gggagcattt tggcagcttt ggagagaaca ytgaagaagg atggaccctt 120 aaggcccaag ggagtgattg gtagagcctc tggagcagga ctgggcctga gatgtgggct 180 ctggaacttg atgctagaga g 201 <210> 84 <211> 201 <212> DNA <213> Homo sapiens <400> 84 ccacgagtgt ccgcaggtgc acagaaacat gggcagtggg atgtttccat gggtcaccta 60 attccagcca aggtggacaa accttcctga acagaatggg ygttgtcagt gtggagaagt 120 atccacaggc tatggccgca gaagctgctg gatggctgag aggcgtctcc aggaaataaa 180 tggggtgcct gttagatagc a 201 <210> 85 <211> 201 <212> DNA <213> Homo sapiens <400> 85 cacatcaaat catctatcaa ttctttctcc atctctcaag cccaactctc ccttgccctc 60 tccagaccat gtgtgcatat cacctgggtc gtcttcattc yctaaatcac ctacgccttt 120 tccttggctt tctcaatttc ctgaggcaca ttcttcaaga gcttgctaag taaagaccca 180 tggagaatac acttttagcc a 201 <210> 86 <211> 201 <212> DNA <213> Homo sapiens <400> 86 agtggcaaag tcactttgca gagcagtgtg cagaaaatta tctgcatctg aaaaagaaca 60 tattgggagt tttattcaga ttgagctgga tacataaatc rtcttggact gaattaacat 120 cttaacaata gtggattttc taattcttga ataccatata tttgttatat gttttaaata 180 tttatgtttg aaaagttttg t 201 <210> 87 <211> 201 <212> DNA <213> Homo sapiens <400> 87 cctttatata gaatgcttat aatagtattg cttatattgt gaaaaaagtt aactagcaga 60 ttgaataaat aaatccatgt gacagggaat tggcagccac raacatattg gtggtagatt 120 aatacataat atataataac atttaattat tttgtatatg gcttactaaa atatataagt 180 ataattaatg acataatgat a 201 <210> 88 <211> 201 <212> DNA <213> Homo sapiens <400> 88 ttttggaata aattggcagg gctcaggaat gtggtagaat gaagaagata agagaattct 60 tgcactctct ggaaaagctt tcagcacaag gagaatggag wttagctttc cctatactgg 120 atgaccctca gtaataatta aggtatttga atgttctgtc cttaacacct gagcttgata 180 aagaacctac tttcagttct t 201 <210> 89 <211> 201 <212> DNA <213> Homo sapiens <400> 89 actgtgaggc accacaccag ccctgggctg cttatgtttc tattactacc tgagaaaaat 60 aaaactctat ctgatttgag tcactattgt tttggggtct yggttatagc agctgacctg 120 tatttaaatt aatacacatg gacacagatg aagcctagtg gaggcttcta agatacaaga 180 tgggggcttg tgtttggaca t 201 <210> 90 <211> 201 <212> DNA <213> Homo sapiens <400> 90 gccaagtaga agctaaagtt ttctcatctc ctcctcttct ctttctctat cgctctttca 60 tgatgcaagt gacaactaat taaataaaac tatctgtgaa wtacatgtgc attgagacct 120 cccagagaca aagatgcaac cttctgccag ccccaaatcc aatctctgct gttccctgtg 180 cccaggggaa cggacccaat g 201 <210> 91 <211> 201 <212> DNA <213> Homo sapiens <400> 91 aaaactctca gaaatgctgg ttaacatata atggacatcc ttttacatat aaatttctag 60 gggccaaaaa aagaagaggg aactgaaaac ctaagtggca rttgtatcca ccctttaatt 120 tcacttgggg cagagaagat aaggtctgtg ccaagtaaag acttggaact gagagcttcc 180 tccccaccaa ccccattgca t 201 <210> 92 <211> 201 <212> DNA <213> Homo sapiens <400> 92 gtggaagagg aactctggaa gcaaacaggc tttagcaaaa gtgatgtaac caaaatggaa 60 tcagatacag attggggaat ctcatcaaca tttacctgta ygcttaaccc agttgtgaca 120 gttagaaaca tgatagttgt ggtgtctggg ggagggtggc atagtggagg ggttgagggt 180 gtggcctctg gagccagggt t 201 <210> 93 <211> 201 <212> DNA <213> Homo sapiens <400> 93 tttgaatgtt ctgtccttaa cacctgagct tgataaagaa cctactttca gttctttgat 60 ctgtgtgtaa cccagcaatg cctctcatcc ctagtgttca mccgagttta tagtctttgc 120 cagagaaaag aaagcatcat tagagaaaca ggccaccagt ttcttctcct gacacttgga 180 tacttcacaa tgtgagttaa a 201 <210> 94 <211> 201 <212> DNA <213> Homo sapiens <400> 94 aaaccaactc ccctggtgct ggaatggttt gtattgtacc atagcatatt atgtggctta 60 tttctaaaat agttagaatt taaatgaatc atgaaaatgc rattaaaaca cactatggtt 120 acaaagattt ttttaaatct aaaacttgga tcttatattt aattgatgat ttgtcattct 180 gtaattttta tattccttgg c 201 <210> 95 <211> 201 <212> DNA <213> Homo sapiens <400> 95 ctcttcccac ttagatttct ctttcattgc ctcatttggc ttcttttcct ctcttggtgt 60 ctctgtgcta acccacttta tttcacttta cactttcttc rgcagcagtg ccatccactc 120 ccatcccttt cttctcttgc cttctctgtt ccttttctat caatgtgctc agcattcctc 180 ctgctcccac taaattggcc a 201 <210> 96 <211> 201 <212> DNA <213> Homo sapiens <400> 96 agtggaagac aagtgttgag acaagccaaa ggttgcgaca aaggcgtaga aaccatccac 60 cttgtcaaat gggctctcat ccttttcaaa gtggagccat kccatgtaga gcagacactg 120 caagcttgat gggaatgagg catatctggc tcaaaagcat gtttatttgt gtgtttttaa 180 aagtgtaaat attgaaccaa c 201 <210> 97 <211> 201 <212> DNA <213> Homo sapiens <400> 97 agggactttt tcactctggg ttagatcatg aaagttaaag tattgggttt gaactgcata 60 aaaaaacaaa caaaatgtgt gtgtcatgat tctagattgt sctaacctat ccagcaagtt 120 caaaactgtg aactattggc taacagacaa gcatgagagt atggctggat aaatgctaga 180 tttatatcat ttgagagaaa a 201 <210> 98 <211> 201 <212> DNA <213> Homo sapiens <400> 98 atattggtac agctttctga atatataaaa gtatattaat aaaaatagag agctgagttt 60 agctttcttt ttgtgacttt tatatttaat aactccaatg watttgatga aacacaaata 120 aatcagctca gaagaattta actgtaatac gtatgctaat attgtttgca ttcaatctgt 180 aagggaatca gaaaccgaag t 201 <210> 99 <211> 201 <212> DNA <213> Homo sapiens <400> 99 agtttggaaa tattacctcc atttttcaga atagtttgag taggattggt atttcttctt 60 ttttatatgt ttggtattac ttcttttata tgtttatcaa wgaagccatc aagttctggg 120 cttttattta ccttgagact tttattagat ctttggtctc attacttcac atgagcctgt 180 tcaggttttg gatttcctca t 201 <210> 100 <211> 201 <212> DNA <213> Homo sapiens <400> 100 ttatatgtta cgttttctac tatttaaatt tcaggcactt cagtggactt caattcatca 60 actcttctac aagagcacgc ttactttctg acactgacct saatcttctt ttcctttgtt 120 agagaataat taaagcctca tatgtagaag cagggctgta aagtcacact gctaagagta 180 ccaatcttgt gactgcagct g 201 <210> 101 <211> 201 <212> DNA <213> Homo sapiens <400> 101 ccagataggg ccttgtagtc cttttaaaaa ctctggcagt tattccagtt gaaaaggtga 60 gcctctgcgg ggttctgatt aaagggttca ctctagttat ygtgtcaaaa tagaaaagaa 120 gcaaggaaga gtaaaagaag gcagaccaat gaggaggtga ttggagcagt ccaggcaaga 180 gtgtttgagg gcttggagca g 201 <210> 102 <211> 201 <212> DNA <213> Homo sapiens <400> 102 aagaacagtt gttttcttta tcttcccttt gagtcctgtt cgaattaaaa tgtatgtttt 60 acaagcattc aaaaaggtaa tggattgttt cacaagtaaa ktcctaagtg ttcagctatt 120 tcagctcttc tttgccattt ggggcttctt acttgttagg tgatttctcc ggccttctat 180 caagcatgat gtgtgtcttc c 201 <210> 103 <211> 201 <212> DNA <213> Homo sapiens <400> 103 ttctctacta agacaactac aatcatatat ttgtgtggaa ttacatatct acataagtgg 60 aaatttattt aatgtcctta catattgtct tgttcattag ytgtctttga aagtcatttt 120 aaaaatcttt gtattacctg cctcaaaagg ttgctaggaa gattggataa aataataaat 180 ataaagcagt gaaatcaatg c 201 <210> 104 <211> 201 <212> DNA <213> Homo sapiens <400> 104 tcatctcttt cgtgtttcta cttaacatta aaaatatgga atacagctat aacaactgtt 60 ttaacatcct tgttggctaa ttctaacatt tctgtcaggt staagtcagt ttggattggt 120 ttttatgttt ttcttctatg ggttgtgttt tcctgctttg catgtcttgt agtctttcat 180 tagatggcag gcactgtgaa t 201 <210> 105 <211> 201 <212> DNA <213> Homo sapiens <400> 105 ggtcacagaa ataacaaggg acttctgtag atcactgcaa gaactttagc atttaccata 60 aatgagattt ttaaaaccac tgaaagcttt tgcaagtaca rgaaaggcat gacctgattt 120 gcattttaaa aaatcatctg gcttctgtct tgaaaataaa atgtgagata aatcggggag 180 accggttagg aggttattgc a 201 <210> 106 <211> 201 <212> DNA <213> Homo sapiens <400> 106 aactcctcac ctcaagtgat ccaccttcct cagcctccca aagtgctggg attacaggcg 60 tgagccactg cactcagcct gtcatctccc atttgcttgt rtaaacgcct gaggctgtgg 120 taggatcctg ccctaaggat agcaattagg gcttggctgc aaggaaggcc cacaggactc 180 aaaatgacaa tgtgtacaca c 201 <210> 107 <211> 201 <212> DNA <213> Homo sapiens <400> 107 aagttctagt tccagtggaa aatttggccc cttggaacta tcagcaacca gcacttcagt 60 catagacctg gctatgttta ccttgtgctt gctttgagtc ytgttgaagt cagttgggaa 120 cacatcatag gtccatcagg attctatgct cgggagacat ggcaagccct atgtgagaag 180 ggtggcaagg aatggcatac a 201 <210> 108 <211> 201 <212> DNA <213> Homo sapiens <400> 108 ggaaatgatt ttgttctttt ttatggctgc atagtattcc atggcatata tgttacgtac 60 cgcagttttt aatccaatcc accaccaatg ggcatcagaa ktgatttaag tctttgctac 120 tgtgaaaaac atatgagtac atgtgtcttt ttgatagcat gatttatttt tctttgggta 180 gatatccagt aatgggattg c 201 <210> 109 <211> 200 <212> DNA <213> Homo sapiens <400> 109 aagcactggt tgacttttgg tgggcaacct gaaagagagc tgtgaatgac atctctcctc 60 cctgctccta actagagagt gtgtctcaga gaactgtgcc rtttaagagc cggaaggcac 120 tctcaaagcc acctggttca atatttccca aagaggagca ttttctgttc atacaatagg 180 atattatttt agcttatgca 200 <210> 110 <211> 201 <212> DNA <213> Homo sapiens <400> 110 cctcctaaat caaccttaga cccacattag ctttcacctg gtgtgactgc tttagttgcc 60 tgttgtctgt gctgctgtcg ctttctccct actaaagcca ycctctgttt tactacctga 120 ttaacctcct ccaagcacac acctaagcac accaccctcc catttacaac cagcaggctc 180 aaaacctttc agtggctccc a 201 <210> 111 <211> 201 <212> DNA <213> Homo sapiens <400> 111 tgctggaggc agagggtgcc aggatgcagc tgtgagccag agaatgccaa ggattttcag 60 ccaccccaaa agctaggaag aggcaaggaa gatttctacc ktgagtttct gatagagtat 120 ggcaatgctc ttgatttggg acttctagcc ttgagaattg tgaaataata catgtatatg 180 ctttaagccc ctccaatttg t 201 <210> 112 <211> 201 <212> DNA <213> Homo sapiens <400> 112 caacacgacc tccctcacat ggctgggggt tgatgctggc tgcaagctgg gagttcagct 60 gggcctatca ggaagggacc tgaactcttc tctatgttga yctctccacg tggctacttg 120 ggcttcctca cagcatggtg actgagttct aagaagaagc actccaagag gcaaaggctg 180 gagctgtgtc atttccgcca t 201 <210> 113 <211> 201 <212> DNA <213> Homo sapiens <400> 113 cttaaataat tctgtgatcc tggctcccga taagaggaaa ttgtctttcc aaaaggcata 60 atagcctcct agggatttgc tttcctttga acaatgaaga rggaggaaaa ggtgtcattg 120 gggatttaaa tttaggagac ttgtttgtac ttaaacttgg cttaataagt gttctttaga 180 gcttgttttt ctttttgtta t 201 <210> 114 <211> 201 <212> DNA <213> Homo sapiens <400> 114 cacacaaaac tgtttctttg gcttgacgaa tacagttaag ccactgctgt ttgttgaaag 60 tgtcattggc ttgtagcgag tgggtctgac tttgggatcc rtttttgaaa ctgactctga 120 agaagttttt aactagaaag gaaaaacaca tgaaaaacaa agtcaatgga cagatccttc 180 ctcaggccaa agagaacaca c 201 <210> 115 <211> 201 <212> DNA <213> Homo sapiens <400> 115 tcctttatag caatgcaaat ggacaccctc cctgtgctgt gtcctgaaat ccctttcaag 60 actggaagtt ggagcaattg cagggctcac ctcatttata yctcttctct tagggtcact 120 gtccttcatt gcctgatgtc cagtgtcttc aaagctatag ctttataggt tttttcatct 180 ttttgttttt atttcagttg c 201 <210> 116 <211> 201 <212> DNA <213> Homo sapiens <400> 116 attcctgtca tattgttgag aacaaattcc ctagtgattc tatgaaataa gatagtattt 60 tatataataa tttggaatcg aatttttctc tgtcatctga mgttacattt cctacttcta 120 ttttatatga aattaatact ctgttttctc acttaattta aatcctgcct gtaaactgtc 180 atgaatgata tgaattaaat a 201 <210> 117 <211> 201 <212> DNA <213> Homo sapiens <400> 117 gaaagtgggt gaacaaaaga cacagggttt tagagacgtg tgtatgtaac tgaagccatt 60 gtatcttgga atacaaggac agttgcacaa aagcagtatg ygatatgtag tcccattcct 120 agatatcatt ggccattttc ctagccagac accaaatagc atgtgataca taaaaataaa 180 ttgatccaat aatagctagg a 201 <210> 118 <211> 201 <212> DNA <213> Homo sapiens <400> 118 tgattgaata aaacacagca gaattagatt tctcaacaaa aatagaacca gaaacacctg 60 tgcacaattg cctagggtgg aataagtgac cttcaggaga rtagggttaa ggtgaagaat 120 tctgaatgcc caaggtggcc tttgggtcag aactggagga atagccttaa tacaaggaga 180 taaccttaca atatttttcc t 201 <210> 119 <211> 201 <212> DNA <213> Homo sapiens <400> 119 atctttttta acataccatt tcaaaacaag gatttgttat gaaaaaattg tctttatccg 60 tcattacttt ttctcatgtt attgtttttt atgcactaaa wagatatgag ttcttgaaat 120 attggtacag ctttctgaat atataaaagt atattaataa aaatagagag ctgagtttag 180 ctttcttttt gtgactttta t 201 <210> 120 <211> 201 <212> DNA <213> Homo sapiens <400> 120 ctttctctcc ctgagatgct ggggtggtca tgagtgcctt ggagaataag ctgaaagccc 60 tagactttgt cttcagagaa aaaatggaca tactcacaca sgtaaaaatt gacattcacc 120 ttcaggccgt ctcctaaaat ctgcctgggt aatagaatag ggtgtctggt aaaatactag 180 cctgggcttg actccagccg g 201 <210> 121 <211> 201 <212> DNA <213> Homo sapiens <400> 121 attagaaagt tttgttgaaa ggacatagat ctaaagatgg ttgagtaggg attacacaaa 60 ttaatgaagg gacaaatgga aatcacattc actcactcga waaacaatta agcacctatt 120 ttgtagcaga gactatagat acaattctgg gttaacaaag aagaatgaga tgtacctgtc 180 atctatttct taacagcttg g 201 <210> 122 <211> 201 <212> DNA <213> Homo sapiens <400> 122 gaagcttgaa aaggagctgg gtgaggcaaa catgcaaatc atgccatgga gattggcaga 60 aaagacgctt gcctccaatt tatggaatgc caagttaatt ygagcccata ggaatgttgt 120 tcccatagat ttagattttt atgaatatat acatatttat gaaagaaaac atttaaagtg 180 tttaattcag taacttatgc a 201 <210> 123 <211> 201 <212> DNA <213> Homo sapiens <400> 123 agaagttaaa tctgtgaaaa aatctttaag tcatcaaata tatacaatta attccacaat 60 ctggaattct cactggcatt tagtcaaaat ggaagttctc ractctcaag aatatattca 120 agaacacaga gtattcagct gtaaatgcaa caattttttc tttgttgatg ggaattgggt 180 gaaatatgat ctttcagatt t 201 <210> 124 <211> 201 <212> DNA <213> Homo sapiens <400> 124 caccacgctg gtcccttcca gaggtgctgt aggtcctctg cctgtaggtc ctctgggcgg 60 actctagttc ctaactgatg gatgatgctg ggccccgctg ytgatgctgc ttaattcata 120 atttggggac tcaagtctct gcaggctcca ctgggccaac ttgcttcctg cagagataca 180 tgttcagact ctactaatta c 201  

Claims (11)

A polynucleotide consisting of SEQ ID NOs: 1 to 124, wherein the polynucleotide comprises one or more polynucleotides selected from polynucleotides comprising the 101 st base of each sequence in the SNP position and consisting of 10 or more consecutive bases, or a complementary poly thereof Single Nucleotide Polymorphism (SNP) for filamentous constitution diagnosis including nucleotides. The polynucleotide of claim 1, wherein the one or more polynucleotides comprise polynucleotides consisting of SEQ ID NOs: 1 to 124, comprising the 101 st base of each sequence which is the position of the SNP, and consisting of 10 or more consecutive bases. Multiple SNPs for filamentous constitution diagnosis selected from the group consisting of 1 to 108 in the following [Table 8]. number Multi SNP Diagnosis One SEQ ID NO: 17, SEQ ID NO: 32, SEQ ID NO: 41 noise 2 SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 34 noise 3 SEQ ID NO: 17, SEQ ID NO: 33, SEQ ID NO: 41 noise 4 SEQ ID NO: 4, SEQ ID NO: 27, SEQ ID NO: 35 noise 5 SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 35 noise 6 SEQ ID NO: 2, SEQ ID NO: 39, SEQ ID NO: 45 noise 7 SEQ ID NO: 22, SEQ ID NO: 34, SEQ ID NO: 42 noise 8 SEQ ID NO: 10, SEQ ID NO: 13, SEQ ID NO: 41 noise 9 SEQ ID NO: 8, SEQ ID NO: 22, SEQ ID NO: 42 noise 10 SEQ ID NO: 2, SEQ ID NO: 7, SEQ ID NO: 47 noise 11 SEQ ID NO: 6, SEQ ID NO: 12, SEQ ID NO: 20 noise 12 SEQ ID NO: 10, SEQ ID NO: 15, SEQ ID NO: 25 noise 13 SEQ ID NO: 10, SEQ ID NO: 13, SEQ ID NO: 43 noise 14 SEQ ID NO: 2, SEQ ID NO: 11, SEQ ID NO: 39 noise 15 SEQ ID NO: 9, SEQ ID NO: 22, SEQ ID NO: 42 noise 16 SEQ ID NO: 27, SEQ ID NO: 30, SEQ ID NO: 44 noise 17 SEQ ID NO: 14, SEQ ID NO: 27, SEQ ID NO: 44 noise 18 SEQ ID NO: 10, SEQ ID NO: 13, SEQ ID NO: 34 noise 19 SEQ ID NO: 18, SEQ ID NO: 22, SEQ ID NO: 34 noise 20 SEQ ID NO: 19, SEQ ID NO: 22, SEQ ID NO: 34 noise 21 SEQ ID NO: 6, SEQ ID NO: 20, SEQ ID NO: 18 noise 22 SEQ ID NO: 6, SEQ ID NO: 20, SEQ ID NO: 19 noise 23 SEQ ID NO: 22, SEQ ID NO: 34, SEQ ID NO: 36 noise 24 SEQ ID NO: 3, SEQ ID NO: 10, SEQ ID NO: 13 noise 25 SEQ ID NO: 28, SEQ ID NO: 31, SEQ ID NO: 34 noise 26 SEQ ID NO: 19, SEQ ID NO: 37, SEQ ID NO: 48 noise 27 SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40 noise 28 SEQ ID NO: 18, SEQ ID NO: 37, SEQ ID NO: 48 noise 29 SEQ ID NO: 5, SEQ ID NO: 39, SEQ ID NO: 40 noise 30 SEQ ID NO: 28, SEQ ID NO: 34, SEQ ID NO: 46 noise 31 SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 26 noise 32 SEQ ID NO: 1, SEQ ID NO: 34, SEQ ID NO: 48 noise 33 SEQ ID NO: 16, SEQ ID NO: 22, SEQ ID NO: 34 noise 34 SEQ ID NO: 16, SEQ ID NO: 24, SEQ ID NO: 26 noise 35 SEQ ID NO: 61, SEQ ID NO: 62, SEQ ID NO: 76 quality 36 SEQ ID NO: 61, SEQ ID NO: 63, SEQ ID NO: 76 quality 37 SEQ ID NO: 54, SEQ ID NO: 64, SEQ ID NO: 67 quality 38 SEQ ID NO: 64, SEQ ID NO: 67, SEQ ID NO: 70 quality 39 SEQ ID NO: 64, SEQ ID NO: 67, SEQ ID NO: 82 quality 40 SEQ ID NO: 51, SEQ ID NO: 65, SEQ ID NO: 79 quality 41 SEQ ID NO: 65, SEQ ID NO: 79, SEQ ID NO: 85 quality 42 SEQ ID NO: 65, SEQ ID NO: 66, SEQ ID NO: 79 quality 43 SEQ ID NO: 65, SEQ ID NO: 79, SEQ ID NO: 80 quality 44 SEQ ID NO: 59, SEQ ID NO: 65, SEQ ID NO: 79 quality 45 SEQ ID NO: 54, SEQ ID NO: 71, SEQ ID NO: 78 quality 46 SEQ ID NO: 70, SEQ ID NO: 71, SEQ ID NO: 78 quality 47 SEQ ID NO: 71, SEQ ID NO: 78, SEQ ID NO: 82 quality 48 SEQ ID NO: 69, SEQ ID NO: 79, SEQ ID NO: 85 quality 49 SEQ ID NO: 66, SEQ ID NO: 69, SEQ ID NO: 79 quality 50 SEQ ID NO: 51, SEQ ID NO: 69, SEQ ID NO: 79 quality 51 SEQ ID NO: 69, SEQ ID NO: 79, SEQ ID NO: 80 quality 52 SEQ ID NO: 50, SEQ ID NO: 54, SEQ ID NO: 78 quality 53 SEQ ID NO: 50, SEQ ID NO: 70, SEQ ID NO: 78 quality 54 SEQ ID NO: 54, SEQ ID NO: 57, SEQ ID NO: 78 quality 55 SEQ ID NO: 57, SEQ ID NO: 70, SEQ ID NO: 78 quality 56 SEQ ID NO: 59, SEQ ID NO: 69, SEQ ID NO: 79 quality 57 SEQ ID NO: 50, SEQ ID NO: 78, SEQ ID NO: 82 quality 58 SEQ ID NO: 57, SEQ ID NO: 78, SEQ ID NO: 82 quality 59 SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 56 quality 60 SEQ ID NO: 79, SEQ ID NO: 75, SEQ ID NO: 80 quality 61 SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 55 quality 62 SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 60 quality 63 SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 72 quality 64 SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 86 quality 65 SEQ ID NO: 51, SEQ ID NO: 74, SEQ ID NO: 79 quality 66 SEQ ID NO: 53, SEQ ID NO: 77, SEQ ID NO: 83 quality 67 SEQ ID NO: 49, SEQ ID NO: 73, SEQ ID NO: 83 quality 68 SEQ ID NO: 54, SEQ ID NO: 78, SEQ ID NO: 84 quality 69 SEQ ID NO: 70, SEQ ID NO: 78, SEQ ID NO: 84 quality 70 SEQ ID NO: 78, SEQ ID NO: 82, SEQ ID NO: 84 quality 71 SEQ ID NO: 52, SEQ ID NO: 68, SEQ ID NO: 81 quality 72 SEQ ID NO: 58, SEQ ID NO: 73, SEQ ID NO: 83 quality 73 SEQ ID NO: 91, SEQ ID NO: 95, SEQ ID NO: 100 Lunar 74 SEQ ID NO: 117, SEQ ID NO: 120, SEQ ID NO: 122 Lunar 75 SEQ ID NO: 91, SEQ ID NO: 98, SEQ ID NO: 103 Lunar 76 SEQ ID NO: 91, SEQ ID NO: 103, SEQ ID NO: 107 Lunar 77 SEQ ID NO: 89, SEQ ID NO: 94, SEQ ID NO: 101 Lunar 78 SEQ ID NO: 91, SEQ ID NO: 103, SEQ ID NO: 116 Lunar 79 SEQ ID NO: 91, SEQ ID NO: 103, SEQ ID NO: 119 Lunar 80 SEQ ID NO: 111, SEQ ID NO: 118, SEQ ID NO: 122 Lunar 81 SEQ ID NO: 104, SEQ ID NO: 111, SEQ ID NO: 113 Lunar 82 SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 114 Lunar 83 SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115 Lunar 84 SEQ ID NO: 105, SEQ ID NO: 113, SEQ ID NO: 123 Lunar 85 SEQ ID NO: 88, SEQ ID NO: 104, SEQ ID NO: 124 Lunar 86 SEQ ID NO: 88, SEQ ID NO: 114, SEQ ID NO: 124 Lunar 87 SEQ ID NO: 88, SEQ ID NO: 115, SEQ ID NO: 124 Lunar 88 SEQ ID NO: 93, SEQ ID NO: 104, SEQ ID NO: 124 Lunar 89 SEQ ID NO: 93, SEQ ID NO: 114, SEQ ID NO: 124 Lunar 90 SEQ ID NO: 93, SEQ ID NO: 115, SEQ ID NO: 124 Lunar 91 SEQ ID NO: 94, SEQ ID NO: 103, SEQ ID NO: 106 Lunar 92 SEQ ID NO: 109, SEQ ID NO: 110, SEQ ID NO: 121 Lunar 93 SEQ ID NO: 87, SEQ ID NO: 99, SEQ ID NO: 104 Lunar 94 SEQ ID NO: 87, SEQ ID NO: 99, SEQ ID NO: 114 Lunar 95 SEQ ID NO: 87, SEQ ID NO: 99, SEQ ID NO: 115 Lunar 96 SEQ ID NO: 92, SEQ ID NO: 111, SEQ ID NO: 118 Lunar 97 SEQ ID NO: 96, SEQ ID NO: 109, SEQ ID NO: 121 Lunar 98 SEQ ID NO: 97, SEQ ID NO: 104, SEQ ID NO: 124 Lunar 99 SEQ ID NO: 97, SEQ ID NO: 114, SEQ ID NO: 124 Lunar 100 SEQ ID NO: 97, SEQ ID NO: 115, SEQ ID NO: 124 Lunar 101 SEQ ID NO: 107, SEQ ID NO: 99, SEQ ID NO: 104 Lunar 102 SEQ ID NO: 107, SEQ ID NO: 99, SEQ ID NO: 114 Lunar 103 SEQ ID NO: 107, SEQ ID NO: 99, SEQ ID NO: 115 Lunar 104 SEQ ID NO: 114, SEQ ID NO: 98, SEQ ID NO: 99 Lunar 105 SEQ ID NO: 115, SEQ ID NO: 98, SEQ ID NO: 99 Lunar 106 SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO: 122 Lunar 107 SEQ ID NO: 90, SEQ ID NO: 102, SEQ ID NO: 103 Lunar 108 SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 120 Lunar 3. The multiple SNP for filamentous constitution diagnosis according to claim 1, wherein the alleles of the 101st base of each sequence, which are the SNP positions of the polynucleotides, are alleles described in [Table 9]. number Multi SNP Allele type Diagnosis One (SEQ ID NO: 17, SEQ ID NO: 32, SEQ ID NO: 41) (AA, AA, AG) noise 2 (SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 34) (GG, GG, AA) noise 3 (SEQ ID NO: 17, SEQ ID NO: 33, SEQ ID NO: 41) (AA, AA, AG) noise 4 (SEQ ID NO: 4, SEQ ID NO: 27, SEQ ID NO: 35) (AA, CC, CC) noise 5 (SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 35) (CC, AA, CC) noise 6 (SEQ ID NO: 2, SEQ ID NO: 39, SEQ ID NO: 45) (CC, TT, AA) noise 7 (SEQ ID NO: 22, SEQ ID NO: 34, SEQ ID NO: 42) (GG, AA, GG) noise 8 (SEQ ID NO: 10, SEQ ID NO: 13, SEQ ID NO: 41) (GG, AA, AG) noise 9 (SEQ ID NO: 8, SEQ ID NO: 22, SEQ ID NO: 42) (CC, GG, GG) noise 10 (SEQ ID NO: 2, SEQ ID NO: 7, SEQ ID NO: 47) (CC, TT, GG) noise 11 (SEQ ID NO: 6, SEQ ID NO: 12, SEQ ID NO: 20) (GG, AA, AA) noise 12 (SEQ ID NO: 10, SEQ ID NO: 15, SEQ ID NO: 25) (GG, AA, TT) noise 13 (SEQ ID NO: 10, SEQ ID NO: 13, SEQ ID NO: 43) (GG, AA, CT) noise 14 (SEQ ID NO: 2, SEQ ID NO: 11, SEQ ID NO: 39) (CC, TT, TT) noise 15 (SEQ ID NO: 9, SEQ ID NO: 22, SEQ ID NO: 42) (TT, GG, GG) noise 16 (SEQ ID NO: 27, SEQ ID NO: 30, SEQ ID NO: 44) (CC, CC, CC) noise 17 (SEQ ID NO: 14, SEQ ID NO: 27, SEQ ID NO: 44) (CC, CC, CC) noise 18 (SEQ ID NO: 10, SEQ ID NO: 13, SEQ ID NO: 34) (GG, AA, AA) noise 19 (SEQ ID NO: 18, SEQ ID NO: 22, SEQ ID NO: 34) (AA, GG, AA) noise 20 (SEQ ID NO: 19, SEQ ID NO: 22, SEQ ID NO: 34) (CC, GG, AA) noise 21 (SEQ ID NO: 6, SEQ ID NO: 20, SEQ ID NO: 18) (GG, AA, AA) noise 22 (SEQ ID NO: 6, SEQ ID NO: 20, SEQ ID NO: 19) (GG, AA, CC) noise 23 (SEQ ID NO: 22, SEQ ID NO: 34, SEQ ID NO: 36) (GG, AA, CC) noise 24 (SEQ ID NO: 3, SEQ ID NO: 10, SEQ ID NO: 13) (AG, GG, AA) noise 25 (SEQ ID NO 28, SEQ ID NO: 31, SEQ ID NO: 34) (AA, AA, AA) noise 26 (SEQ ID NO: 19, SEQ ID NO: 37, SEQ ID NO: 48) (CC, CC, GG) noise 27 (SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40) (GG, TT, AG) noise 28 (SEQ ID NO: 18, SEQ ID NO: 37, SEQ ID NO: 48) (AA, CC, GG) noise 29 (SEQ ID NO: 5, SEQ ID NO: 39, SEQ ID NO: 40) (GG, TT, AG) noise 30 (SEQ ID NO 28, SEQ ID NO: 34, SEQ ID NO: 46) (AA, AA, GG) noise 31 (SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 26) (CC, AA, CC) noise 32 (SEQ ID NO: 1, SEQ ID NO: 34, SEQ ID NO: 48) (TT, AA, GG) noise 33 (SEQ ID NO: 16, SEQ ID NO: 22, SEQ ID NO: 34) (GG, GG, AA) noise 34 (SEQ ID NO: 16, SEQ ID NO: 24, SEQ ID NO: 26) (GG, AA, CC) noise 35 (SEQ ID NO: 61, SEQ ID NO: 62, SEQ ID NO: 76) (AT, CC, CC) quality 36 (SEQ ID NO: 61, SEQ ID NO: 63, SEQ ID NO: 76) (AT, GG, CC) quality 37 (SEQ ID NO: 54, SEQ ID NO: 64, SEQ ID NO: 67) (TT, GG, CT) quality 38 (SEQ ID NO: 64, SEQ ID NO: 67, SEQ ID NO: 70) (GG, CT, GG) quality 39 (SEQ ID NO: 64, SEQ ID NO: 67, SEQ ID NO: 82) (GG, CT, TT) quality 40 (SEQ ID NO: 51, SEQ ID NO: 65, SEQ ID NO: 79) (TT, AA, AA) quality 41 (SEQ ID NO: 65, SEQ ID NO: 79, SEQ ID NO: 85) (AA, AA, TT) quality 42 (SEQ ID NO: 65, SEQ ID NO: 66, SEQ ID NO: 79) (AA, TT, AA) quality 43 (SEQ ID NO: 65, SEQ ID NO: 79, SEQ ID NO: 80) (AA, AA, TT) quality 44 (SEQ ID NO: 59, SEQ ID NO: 65, SEQ ID NO: 79) (GG, AA, AA) quality 45 (SEQ ID NO 54, SEQ ID NO 71, SEQ ID NO 78) (TT, GG, AG) quality 46 (SEQ ID NO: 70, SEQ ID NO: 71, SEQ ID NO: 78) (GG, GG, AG) quality 47 (SEQ ID NO: 71, SEQ ID NO: 78, SEQ ID NO: 82) (GG, AG, TT) quality 48 (SEQ ID NO: 69, SEQ ID NO: 79, SEQ ID NO: 85) (TT, AA, TT) quality 49 (SEQ ID NO: 66, SEQ ID NO: 69, SEQ ID NO: 79) (TT, TT, AA) quality 50 (SEQ ID NO: 51, SEQ ID NO: 69, SEQ ID NO: 79) (TT, TT, AA) quality 51 (SEQ ID NO: 69, SEQ ID NO: 79, SEQ ID NO: 80) (TT, AA, TT) quality 52 (SEQ ID NO: 50, SEQ ID NO: 54, SEQ ID NO: 78) (AA, TT, AG) quality 53 (SEQ ID NO: 50, SEQ ID NO: 70, SEQ ID NO: 78) (AA, GG, AG) quality 54 (SEQ ID NO 54, SEQ ID NO 57, SEQ ID NO 78) (TT, GG, AG) quality 55 (SEQ ID NO: 57, SEQ ID NO: 70, SEQ ID NO: 78) (GG, GG, AG) quality 56 (SEQ ID NO: 59, SEQ ID NO: 69, SEQ ID NO: 79) (GG, TT, AA) quality 57 (SEQ ID NO: 50, SEQ ID NO: 78, SEQ ID NO: 82) (AA, AG, TT) quality 58 (SEQ ID NO: 57, SEQ ID NO: 78, SEQ ID NO: 82) (GG, AG, TT) quality 59 (SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 56) (AA, TT, TT) quality 60 (SEQ ID NO: 79, SEQ ID NO: 75, SEQ ID NO: 80) (AA, AA, TT) quality 61 (SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 55) (AA, TT, GG) quality 62 (SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 60) (AA, TT, CC) quality 63 (SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 72) (AA, TT, CC) quality 64 (SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 86) (AA, TT, CC) quality 65 (SEQ ID NO: 51, SEQ ID NO: 74, SEQ ID NO: 79) (TT, GG, AA) quality 66 (SEQ ID NO: 53, SEQ ID NO: 77, SEQ ID NO: 83) (AG, CC, CC) quality 67 (SEQ ID NO: 49, SEQ ID NO: 73, SEQ ID NO: 83) (AA, AA, TT) quality 68 (SEQ ID NO: 54, SEQ ID NO: 78, SEQ ID NO: 84) (TT, AG, AA) quality 69 (SEQ ID NO: 70, SEQ ID NO: 78, SEQ ID NO: 84) (GG, AG, AA) quality 70 (SEQ ID NO: 78, SEQ ID NO: 82, SEQ ID NO: 84) (AG, TT, AA) quality 71 (SEQ ID NO: 52, SEQ ID NO: 68, SEQ ID NO: 81) (TT, CT, CC) quality 72 (SEQ ID NO 58, SEQ ID NO: 73, SEQ ID NO: 83) (CG, AA, TT) quality 73 (SEQ ID NO: 91, SEQ ID NO: 95, SEQ ID NO: 100) (TT, CT, CG) Lunar 74 (SEQ ID NO: 117, SEQ ID NO: 120, SEQ ID NO: 122) (AG, CC, GG) Lunar 75 (SEQ ID NO: 91, SEQ ID NO: 98, SEQ ID NO: 103) (TT, AT, AA) Lunar 76 (SEQ ID NO: 91, SEQ ID NO: 103, SEQ ID NO: 107) (TT, AA, AG) Lunar 77 (SEQ ID NO: 89, SEQ ID NO: 94, SEQ ID NO: 101) (AG, AG, AA) Lunar 78 (SEQ ID NO: 91, SEQ ID NO: 103, SEQ ID NO: 116) (TT, AA, AC) Lunar 79 (SEQ ID NO: 91, SEQ ID NO: 103, SEQ ID NO: 119) (TT, AA, AT) Lunar 80 (SEQ ID NO: 111, SEQ ID NO: 118, SEQ ID NO: 122) (CC, AG, GG) Lunar 81 (SEQ ID NO: 104, SEQ ID NO: 111, SEQ ID NO: 113) (GG, CC, AA) Lunar 82 (SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 114) (CC, AA, TT) Lunar 83 (SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115) (CC, AA, CC) Lunar 84 (SEQ ID NO: 105, SEQ ID NO: 113, SEQ ID NO: 123) (AG, AA, AG) Lunar 85 (SEQ ID NO: 88, SEQ ID NO: 104, SEQ ID NO: 124) (TT, GG, AG) Lunar 86 (SEQ ID NO: 88, SEQ ID NO: 114, SEQ ID NO: 124) (TT, TT, AG) Lunar 87 (SEQ ID NO: 88, SEQ ID NO: 115, SEQ ID NO: 124) (TT, CC, AG) Lunar 88 (SEQ ID NO: 93, SEQ ID NO: 104, SEQ ID NO: 124) (CC, GG, AG) Lunar 89 (SEQ ID NO: 93, SEQ ID NO: 114, SEQ ID NO: 124) (CC, TT, AG) Lunar 90 (SEQ ID NO: 93, SEQ ID NO: 115, SEQ ID NO: 124) (CC, CC, AG) Lunar 91 (SEQ ID NO: 94, SEQ ID NO: 103, SEQ ID NO: 106) (AG, AA, CC) Lunar 92 (SEQ ID NO: 109, SEQ ID NO: 110, SEQ ID NO: 121) (CT, CC, TT) Lunar 93 (SEQ ID NO: 87, SEQ ID NO: 99, SEQ ID NO: 104) (AG, AA, GG) Lunar 94 (SEQ ID NO: 87, SEQ ID NO: 99, SEQ ID NO: 114) (AG, AA, TT) Lunar 95 (SEQ ID NO: 87, SEQ ID NO: 99, SEQ ID NO: 115) (AG, AA, CC) Lunar 96 (SEQ ID NO: 92, SEQ ID NO: 111, SEQ ID NO: 118) (CC, CC, AG) Lunar 97 (SEQ ID NO: 96, SEQ ID NO: 109, SEQ ID NO: 121) (CC, CT, TT) Lunar 98 (SEQ ID NO: 97, SEQ ID NO: 104, SEQ ID NO: 124) (CC, GG, AG) Lunar 99 (SEQ ID NO: 97, SEQ ID NO: 114, SEQ ID NO: 124) (CC, TT, AG) Lunar 100 (SEQ ID NO: 97, SEQ ID NO: 115, SEQ ID NO: 124) (CC, CC, AG) Lunar 101 (SEQ ID NO: 107, SEQ ID NO: 99, SEQ ID NO: 104) (AG, AA, GG) Lunar 102 (SEQ ID NO: 107, SEQ ID NO: 99, SEQ ID NO: 114) (AG, AA, TT) Lunar 103 (SEQ ID NO: 107, SEQ ID NO: 99, SEQ ID NO: 115) (AG, AA, CC) Lunar 104 (SEQ ID NO: 114, SEQ ID NO: 98, SEQ ID NO: 99) (TT, AT, AA) Lunar 105 (SEQ ID NO: 115, SEQ ID NO: 98, SEQ ID NO: 99) (CC, AT, AA) Lunar 106 (SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO: 122) (CC, CT, GG) Lunar 107 (SEQ ID NO: 90, SEQ ID NO: 102, SEQ ID NO: 103) (AA, GG, AA) Lunar 108 (SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 120) (GT, CC, CG) Lunar The method of claim 1, wherein the filamentous constitution is noise, Soyangin, Taeinin and Sunin among the noise, Soyangin and Taeumin multiple SNP for the diagnosis of filamentous constitution. The multiple SNP for diagnosis of filamentous constitution according to claim 1, wherein the 10 or more consecutive bases are 10 to 100 consecutive bases. A microarray for filamentous constitution comprising a polynucleotide of the multiple SNP of claim 1 or a polypeptide encoded by the same. 7. The filamentous constitution diagnostic microarray according to claim 6, wherein the microarray is coated with one active group selected from the group consisting of amino-silane, poly-L-lysine and aldehyde on a substrate. 8. The microarray for filamentous constitution diagnosis according to claim 7, wherein the substrate is any one selected from the group consisting of silicon wafer, glass, quartz, metal and plastic. Sasang constitution diagnostic kit comprising the microarray of claim 6. 1) separating the nucleic acid sample from the subject; 2) labeling the nucleic acid sample with a fluorescent material; 3) hybridizing a nucleic acid sample labeled with a fluorescent substance with the microarray of claim 7; 3) analyzing the reacted microarrays to determine the genotype of each polymorphic site of at least one polynucleotide selected from SEQ ID NOs: 1 to 124; And 4) A method for detecting multiple SNPs according to claim 1 for diagnosing filamentous constitution including using the genotype to determine filamentous constitution. The phosphor of step 3) is any one selected from the group consisting of Cy3, Cy5, poly L-lysine-fluorescein isothiocyanate (FITC), rhodamine-B-isothiocyanate (RITC), and rhodamine (rhodamine). Method characterized in that.