KR20100122359A - An icecream having anti-atopic function - Google Patents

Abstract

PURPOSE: An ice cream having anti-atopic function is provided to offer an ice cream having anti-atopic effect by including Cordyceps bassiana extracts. CONSTITUTION: An anti-atopic functional ice cream composition includes the extract by organic solvent of the Cordyceps bassiana to the active ingredient. The organic solvent is either butanol or ethanol. The ice cream product contains the ice cream composition.

Description

Anti ice cream functional ice cream composition {An icecream having Anti-Atopic function}

The present invention discloses an anti-atopic functional ice cream composition comprising yellow mushrooms of Cordyceps sinensis extract as an active ingredient.

Atopic dermatitis is a chronic skin disease accompanied by dry skin, keratinosis and itching, which affects 0.5 to 1% of the population and 5 to 10% of children, and the number of patients is increasing. Infections with atopic dermatitis significantly increase infiltration of immune-related cells such as macrophages, mast cells and Th lymphocytes. In addition, in patients with atopic dermatitis, blood IgE levels increase significantly because the number of Th2 cells increases and IL-4 secreted by these cells stimulates B lymphocytes to promote IgE secretion. Therefore, atopic dermatitis is classified as a Th2-type skin disease associated with abnormalities of the immune system. Atopic dermatitis inflicts a lot of economic damage along with physical and mental pain for the patient, but no specific drug has yet been found to treat the disease. Currently, steroids and anti-histamines are used, but long-term use of these drugs causes serious side effects, so they need to replace them.

Cordyceps sinensis is a fungal insect parasite, belonging to the genus Cordyceps . Currently, 300 species are known to be distributed around the world. Cordyceps sinensis invade insect larvae, chrysalis, adult insects, etc., and kills insects, forming a fruiting body and fruit as a host. Cordyceps sinensis has long been known as a elixir of immortality in China and is widely used as a herbal medicine for the treatment of respiratory diseases including asthma and liver diseases including jaundice. In recent years, research on the enhancement of immunity of Cordyceps sinensis has been extensively conducted. There are Cordyceps bat moth (C. sinensis) extract, Cordyceps militaris reported that also acts as well as act as an anti-inflammatory substance or extract composed mainly of the cordycepin immunoregulatory material of the fungus (C. militaris). Therefore, it is expected that Cordyceps sinensis may contain an inhibitory effect against atopic dermatitis, an immune inflammatory disease, but no systematic and scientific findings are available.

C. bassiana ( C. bassiana ), a type of cordyceps, is the first parasitic fungus to be found in the genus Butterfly Lepidoptera. Beauveria bassiana , a form that appears during the life cycle of yellow bunched cordyceps, has been used in China and Korea (Dongeuibogam) for a long time for the treatment of neurological diseases, cancers, skin infections, wounds, etc. Nothing is known about it.

Recently, strains have been isolated from yellow bunched caterpillar parasites of butterfly larvae and cultivation methods to fruiting bodies have been established. In the present invention, to find a component that inhibits atopic dermatitis from artificially cultivated yellow bunch Cordyceps sinensis and to investigate its mechanism of action. As a model of atopic dermatitis, NC / Nga mice established in obedience of Japanese fancy mice were used and 2,4-dinitrofluorobenzene (DNFB) was used to induce atopic dermatitis. This mouse not only has a naturally occurring atopic dermatitis when raised in a normal environment, but also has a property of enhancing atopic dermatitis by repeatedly applying a chemical antigen such as DNFB to the skin.

An object of the present invention is to provide a yellow bunch Cordyceps sinensis extract to be made in view of the above points.

Another object of the present invention is to provide an anti-atopic functional ice cream composition comprising the yellow bunch Cordyceps sinensis extract as an active ingredient.

Still another object of the present invention is to provide an ice cream using the ice cream composition.

The object of the present invention comprises the steps of extracting the biologically active substance of yellow cordyceps sinensis; Verifying the effect of the Yellow Cordyceps sinensis extract on atopic dermatitis; Calculating atopic dermatitis scores; Affected skin thickness measurement step; Measuring serum IgE concentration and histamine concentration; It was achieved through the production of ice cream composition and evaluation step.

The present invention has an effect of providing an anti-atopic functional ice cream composition comprising the yellow bunch Cordyceps sinensis extract as an active ingredient, and has an excellent effect of providing an anti-atopic functional ice cream using the same.

Hereinafter, the specific details of the present invention will be described in detail with reference to some examples, but the scope of the present invention is not limited thereto, and a person skilled in the art will be able to imitate by changing the numerical value and material method of the ice cream composition.

Specific details of the present invention will be described in detail with reference to the accompanying drawings and tables.

Extraction of Biologically Active Compounds from Yellow Bunny Cordyceps

Yellow bunch Cordyceps sinensis: In this experiment, yellow bunch Cordyceps sinus cultivated in Maschtec was dried and used as a sample.

Water extraction and ethanol extraction: Mix 3 times distilled water or ethanol equivalent to 10 times the weight of the sample, heat-extract for 4 hours, concentrate the extract, and freeze-dried to extract water extract (CBWE) and ethanol extract (CBEE), respectively. Got it.

Preparation of Butanol Fraction: A sample of dried Yellow Cordyceps sinensis and ethanol corresponding to 20 times the weight of the sample were mixed, extracted by heating for 4 hours, and the extract was concentrated under reduced pressure and lyophilized. The dried ethanol extract was dissolved in a hexane solution, and then the hexane layer was separated and removed. N-butanol was mixed with the remaining solution, and the butanol layer was separated and concentrated, and then lyophilized to obtain a butanol fraction (CBBF) (FIG. 1).

Validation of the Healing Effect of Each Extract of Yellow Cordyceps sinensis on Atopic Dermatitis

Induction of atopic dermatitis in experimental animals: In the present invention, NC / Nga mouse (male, 6 weeks old), which is a model animal of atopic dermatitis, was used. The animals were used for experiments after adapting the animals for 1 week in a normal breeding room where the temperature and humidity were maintained at 20 ° C. and 50%, and the contrast was changed every 12 hours. The back of the NC / Nga mouse was epilated and left for 24 hours to heal fine wounds on the skin. 150 μl of acetone / olive oil (3: 1) containing 0.2% of 2,4-dinitrofluorobenzene (DNFB) was applied twice a week for 4 weeks to induce atopic dermatitis. Was extended to 6 weeks.

Treatment of Various Extracts of Yellow Cordyceps sinensis for Atopic Dermatitis: In the normal control group, only the back area was epilated, and in the atopy group, dermatitis was induced and only vaseline was applied. In the experimental group, atopic dermatitis was induced, and then mixed with vaseline, CBWE, CBEE, and CBBF were applied twice a day for 12 days.

Calculation of atopic dermatitis score

After the application of each extract of Cordyceps sinensis, the affected area was observed, and gross changes such as erythema, bleeding, and scab were evaluated in 1 to 10 stages, which were used as dermatitis scores.

Wound epidermal layer thickness measurement

In the center of the affected area, skin tissue was collected in a size of 1 cm x 1 cm, fixed in a 10% formaldehyde solution, embedded with paraffin, and a section having a thickness of 4 μm was prepared. The tissue sections were stained by hematoxylin-eosin method, and then magnified 200 times using an optical microscope, and the thickness of the epidermal layer was measured by selecting 5 visual fields.

Measurement of Serum IgE Concentration and Histamine Concentration

Blood was collected from the heart and serum IgE and histamine concentrations were measured by ELISA.

Statistical processing of measured values

All measurements obtained in this experiment are presented as mean ± standard error. The mean difference was statistically tested using Student's t test and the significance level was less than 5%.

Effect of CBWE and CBEE on Atopic Dermatitis

Atopic dermatitis was induced over 4 weeks, and water extract (CBWE) and ethanol extract (CBEE) from yellow cordyceps were applied to the affected area for 12 days at a concentration of 10 mg / g. As a result, the increased dermatitis score, epidermal layer thickness, and serum IgE concentration were not affected by CBWE but decreased by CBEE [Table 1].

Effect of CBWE and CBEE on the indicators of each dermatitis increased by atopic dermatitis. DNFB is applied for 4 weeks to induce atopic dermatitis. Each extract is mixed in a vaseline at a concentration of 10 mg / g and included in the affected area for 12 days. Experimental Value: 5 Means ± Standard Error.

TABLE 1

Atopy control CBWE CBEE Dermatitis score 7.20 ± 0.37 6.60 ± 1.44 7.40 ± 0.40 Epidermal thickness (μm) 221.32 ± 7.25 194.47 ± 30.49 207.52 ± 19.00 Serum IgE level (μg / ml) 9.20 ± 1.14 11.99 ± 4.26 7.85 ± 1.60

Concentration-dependent Effects of CBBF on Atopic Dermatitis

Atopic dermatitis was induced over 4 weeks and CBBF was applied to the affected area for 12 days at a concentration of 5, 10 or 20 mg / g. As a result, the dermatitis score, epidermal layer thickness, and blood IgE concentration increased by atopic dermatitis were concentration-dependently reduced by CBBF [FIG. 2, FIG. 3].

Effect of CBBF on the Duration of Atopic Dermatitis

In order to observe the improvement effect of CBBF according to the induction period of atopic dermatitis, the induction period of atopic dermatitis was extended to 6 weeks and CBBF was applied to the affected area at a concentration of 10 mg / g for 12 days to obtain the following results.

1) Gross and histological changes of the affected area: As shown in FIG. 4, when the induction period of atopic dermatitis was extended to 6 weeks, the dermatitis score did not increase slightly, but the epidermal layer thickness increased significantly. Treatment with CBBF reduced both the dermatitis score and epidermal layer thickness, and caused both measurements for four weeks (54% decrease in dermatitis score, 64% decrease in epidermal layer thickness) (42% decrease in dermatitis score, epidermis). A 58% reduction in layer thickness).

2) Changes in serum IgE and serum histamine concentrations: As shown in FIG. 5, both serum IgE and serum histamine concentrations increased significantly when prolonged atopic dermatitis induction period was 6 weeks. Treatment with CBBF reduced both serum IgE and serum histamine levels, and both measurements were induced for 6 weeks (69% decrease in IgE, 70% decrease in histamine) and 52% in IgE and 40% in histamine. Decreased more than).

Hereinafter, the preparation examples and sensory test results of the anti-atopic ice cream and ice cream containing the yellow bund Cordyceps ethanol extract of the present invention.

First, the ice cream composition (Table 2) to which the yellow bund Cordyceps ethanol extract is added and a preparation method thereof will be described.

TABLE 2 ICE CREAM COMPOSITION

ingredient Composition ratio (part by weight) milk
Dairy products (whipped cream, skim milk powder)
stabilizator
Emulsifier
Sugars (sugar, fructose)
Yellow Bunny Cordyceps Sinensis Extract 25-30
5-20
0.1 ~ 2
0.1 ~ 1
2 ~ 20
10-25

The present invention adds an appropriate amount of nuts and flavors to the ice cream composition and freezes at -2 to -5 ° C temperature for 10 to 20 minutes in an ice cream maker.

The frozen ice cream was filled into a conventional ice cream container, frozen at -20 ° C, and the sensory characteristics were compared with the conventional product (control) (Table 3).

[Table 3]

Kinds Softness Color sweetness Flavor General Symbol Invention ice cream 7.6 8.2 7.4 7.8 7.6 Control 6.5 6.4 7.3 7.2 7.5

The results of sensory evaluation of 20 college students were as shown in Table 3 above. The higher the score from 1 to 9, the better the sensory beauty.

In the experimental group containing the present invention yellow bunched Cordyceps Sinensis extract, it was judged to be effective as an ice cream for improving atopic dermatitis prevention disease in comparison with a normal product in terms of functional and general symbols.

Ice cream (Table 4) using the anti-atopic functional food composition of the present invention and the evaluation results related thereto are as follows.

Table 4 Ice Cream Composition

ingredient Composition ratio (part by weight) Yellow Bunny Cordyceps Sinensis Extract
Liquid sugar
Aspartame
Citric acid
Vitamin C
Grapefruit Juice Concentrate
Spices
water 1-5
10-15
0.1-0.5
4 ~ 4.5
0.1-0.15
5 ~ 5.5
4 ~ 4.5
100

Actually, 2 kg of the present invention Yellow Bundo Cordyceps sinensis ethanol extract, 12 kg of isomerized sugar, 250 g of aspartame, 4.2 kg of citric acid, 0.12 kg of vitamin C, 5.2 kg of grapefruit concentrate, 4.2 kg of fragrance, dissolved in 100 L of water, sugar content 1.5 ° BX, acidity 0.43 It was made into a homogeneous solution (w / w), sterilized at 85 ° C. for 15 seconds, cooled to 5 ° C., and flowed into an oil-type ice maker to obtain an ice cream after 20 minutes. The ice cream prepared as described above was compared with a control product which did not use the Yellow Bunny Cordyceps ethanol extract by performing a sensory test (Table 5).

[Table 5] Sensory test

division Color group flavor Scent Overall Symbol Example 3.3 3.8 3.9 4.0 4.0 Control 3.1 3.5 3.7 3.5 3.3

The average value of the evaluation results of color, texture, taste, fragrance, and general symbol scores was calculated using a score comparison method for 20 sensory test staff consisting of college students. Since it can be confirmed that it is not, it can be seen that there is no inferiority with the anti-atopic functional product of the present invention.

The ice cream composition and the ice cream product comprising the yellow bund Cordyceps sinensis extract as an active ingredient are effective in the ice cream industry, such as anti-atopic functional ice cream, because they have an effect of improving atopic dermatitis by inhibiting Th2 cell immune response in the skin.

1 is a flow chart of the extraction of bioactive materials from yellow bunch Cordyceps.

2 is a graph showing the effect of CBBF on each dermatitis index increased by atopic dermatitis.

3 is a photograph showing the effect of CBBF on the histological changes of the skin caused by atopic dermatitis. (Magnification × 100, Scale bar = 100um)

4 is a graph showing the effect of CBBF on the increased dermatitis score and epidermal layer thickness by atopic dermatitis.

Figure 5 is a graph showing the effect of CBBF on the concentration of fluoride IgE and histamine increased by atopic dermatitis.

Claims (4)

Organic Solvent Extract of Yellow Bunch Cordyceps. The organic solvent of claim 1, wherein the organic solvent extract is any one of Butanol or ethanol. An ice cream composition comprising the organic solvent extract of claim 1 or 2 as an active ingredient. An ice cream product containing the ice cream composition of claim 3.

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