KR20100104144A - Skin whitening composition comprising horse fats and horse placenta extracts encapsulated in water soluble nanoliposome - Google Patents

Abstract

PURPOSE: A composition containing water soluble nano liposome having horse fat and horse placenta extract is provided to enhance stability without skin irritation and to ensure skin whitening effect. CONSTITUTION: A composition for skin whitening comprises a 0.001-10 weight% of nano liposome containing 2-10 weight% of horse fat and 1-5 weight% of horse placenta extract as active ingredients. The horse fat is contained I 2-10 weight% based on total weight. The nano liposome contains emulsifier, cluster dextrin and water soluble solvent. A method for preparing water soluble nano liposome comprises: a step of dissolving horse placenta extract in water soluble solvent; a step of adding cluster dextrin and heating at 50-60°C; a step of gradually adding horse fat and emulsifier; and a step of adding amino acid and stirring at 0~4°C.

Description

Skin whitening composition comprising horse fats and horse placenta extracts encapsulated in water soluble nanoliposome}

The present invention relates to a composition for skin whitening comprising a water-soluble nanoliposome containing horse oil and placenta extract as an active ingredient.

Human skin color is determined by the concentration and distribution of melanin pigments inside the skin. Under normal conditions, melanin overproduction does not occur, but when the skin reacts to external stimuli such as ultraviolet rays, environmental pollution, stress, or internal factors such as hormones or inflammation factors, the melanin is overproduced, and it is not normally released from the skin. Accumulation on the skin causes pigmentation. As such, pigmentation of the skin may be caused by various factors such as hormonal abnormalities in the body after the inflammatory response of the skin, hereditary diseases, and ultraviolet irradiation, and the main factors are due to melanin synthesis abnormalities and distribution abnormalities.

The main function of melanin is to remove oxygen radicals and protect the skin from the damage thereby. Therefore, high levels of melanin mean that it has an effective response system to protect the skin from physical and chemical toxic substances. Melanin is produced by the action of enzymes and spontaneous oxidation after tyrosine is converted to dopaquinone by the action of tyrosinase in melanocytes. Therefore, various methods have been applied to inhibit the production of melanin to maintain or improve skin whitening. For example, core carbohydrates such as glucosamine, which are essential for the activity of tyrosinase or for use in cosmetic compositions containing light scattering agents or light blockers, in order to remove the main cause of melanin production by blocking UV rays. It inhibits synthesis and inhibits melanin production. Alternatively, using a substance that inhibits the activity of tyrosinase, an enzyme involved in melanogenesis, such as kojic acid or arbutin, or melanocytes, which are cells that produce melanin, such as hydroquinone. A method of interfering with the division of melanocytes using a substance having a specific toxicity to the has been used. In addition, there is a method of reducing the produced melanin to decolorize.

Typically, hydroquinone, kojic acid, ascorbic acid and derivatives thereof obtained from natural products are used for skin whitening by inhibiting tyrosinase activity, blemishes caused by sun, freckles, etc. It was used as an additive in cosmetics such as ointments and essences for the purpose of improving skin hyperpigmentation. However, hydroquinone exhibits a predetermined whitening effect, but due to its high irritation to the skin, it cannot be used as a cosmetic due to safety problems, and kojic acid is unstable in a solution, which complicates the manufacturing process of the cosmetic. In addition, ascorbic acid is easy to oxidize, and cosmetics containing the same may cause discoloration and deodorization, which is not suitable as a whitening raw material. Therefore, there is still a need for an active substance for skin whitening that suppresses the excessive production of melanin pigments, has low toxicity to the skin and high stability.

Therefore, the present inventors search for skin whitening active substances having high whitening activity and stability and safety, and the nanoliposomes containing horse oil and placenta extract prevent the consequent phenomenon of horse oil and placenta extract to enhance stability, and improve the cells. The present invention was found to have high melanogenesis inhibitory activity without toxicity.

It is an object of the present invention to provide a composition for skin whitening comprising nanoliposomes containing horse oil and placenta extract having high stability, safety and skin whitening activity as an active ingredient.

Still another object of the present invention is to provide a method for preparing a water-soluble nanoliposome containing horse oil and placenta extract.

In order to achieve the above object, the present invention provides a composition for skin whitening comprising nanoliposome containing horse oil and placenta extract as an active ingredient.

Placenta extract has the effect of inhibiting the activity of tyrosinase, an enzyme that promotes the production of melanin granules from tyrosine, inhibits melanin pigment formation and promotes the release of cells containing melanin pigment. It is effective in prevention and improvement, and shows whitening as well as preventing pigmentation (Mallick S et el., Pigment Cell Res. Feb 18 (1), 25-33, 2005). However, horse placenta extract is excellent in whitening efficacy, but due to its peculiar smell and instability, it is easily transformed during product production, and has a disadvantage in that irritation occurs in the skin when used at high concentrations. Therefore, in order to commercialize horse placenta extract, it is necessary to increase stability and reduce skin irritation. The placenta extract can be encapsulated in liposome form to stabilize the placenta extract and reduce skin toxicity. Nanoliposomes have been used as useful formulations to increase stability and bioavailability because they increase the skin penetration of the active ingredient contained therein and can also increase the direct effect on cells.

Horse oil is a natural oil containing 60% or more of unsaturated fatty acids, such as oleic acid, linoleic acid, and palmitooleic acid, which are known to be effective in treating wounds and burns, skin regeneration, anti-inflammatory and atopic dermatitis. Is known to contain alpha linolenic acid, which is effective in protecting damaged skin (Takamura N, Takahashi K, Tanaka H et al. Dietary, but not topical, alpha-linolenic acid suppress UVB-induced skin injury in hairless mice when compared with linoleic acids.Phtochem Phtobiol 2002; 76: 657-63). Therefore, nanoliposomes of horse placenta extract using horse oil can improve the stability of horse placenta extract, reduce inflammation, and reduce skin irritation.

Horse oil and horse placenta extract used to prepare nanoliposomes containing horse oil and horse placenta extract according to an embodiment of the present invention was prepared by a method filed by the applicant (Korean Patent Application Publication No. 2008-01000313 And 2008-0103490). Specifically, removing the crystals formed by adding urea to horse oil, adding activated carbon and activated clay to the obtained filtrate and reacting under vacuum, removing activated carbon and activated clay by filtration, and the filtrate obtained at room temperature Purified horse oil can be obtained by a process comprising the step of cooling to and adding water to the filtrate and performing thin film evaporation under vacuum. Horse oil obtained by the above method has no color and smell, and horse oil has high efficacy and bioabsorption rate. The horse's placenta extract is subjected to the first reaction by adding a proteolytic enzyme such as papain to the horse's placenta, the second reaction by adding the proteolytic enzyme to the reactant, and adding activated charcoal to the reactant under the odor and color under vacuum. The placenta extract can be obtained by a method comprising the step of removing the. The horse placenta extract obtained by the above method minimizes the destruction of the bioactive substance in the horse placenta and increases the content of the active ingredient.

In one embodiment of the present invention, the nanoliposome containing horse oil and placenta extract contained in the composition for skin whitening may be included in 0.001 to 10% by weight, more preferably 0.01 to 5% by weight relative to the total weight of the composition May be included as a%. When the content of nanoliposome containing horse oil and placenta extract is less than 0.001% by weight relative to the total weight of the composition, the skin whitening effect is insignificant. .

In one embodiment of the present invention, horse oil and horse placenta extract containing the horse oil and horse placenta extract content in the nanoliposomes are not limited, but preferably placenta extract having a whitening function in consideration of the phase stability of liposomes 1 to 5% by weight relative to the total weight of silver nanoliposomes, horse oil with anti-inflammatory, burn, wound healing, anti-atopic action is 2 to 10% by weight relative to the total weight of nanoliposomes in consideration of the phase stability of liposomes during liposome preparation May be%.

In one embodiment of the invention, the nanoliposomes containing horse oil and placenta extract may comprise an emulsifier, cluster dextrin and a hydrophilic solvent.

In another embodiment of the invention, the nanoliposomes containing horse oil and placenta extract may be water-soluble nanoliposomes further comprising amino acids.

By using amino acids and cluster dextrins together in the preparation of nanoliposomes containing horse oil and placenta extract, the nanoliposomes are made water-soluble so that liposomes can be used in water-soluble products, and the nanoliposomes also inhibit the unexpected melanin production. It was confirmed to have an effect. That is, the water-soluble nanoliposomes containing horse oil and horse placenta extract according to one embodiment of the present invention were found to greatly increase the efficacy compared to the content of horse placenta extract having a skin whitening effect.

In one embodiment of the present invention, the water-soluble nanoliposomes containing horse oil and placenta extract used as active ingredients in the composition for skin whitening are encapsulated at the molecular level using a cluster dextrin, the molecular capsule formulation And liposomes may be formed using an emulsifier in the formed molecular capsule, and then converted into a water-soluble lipid which further increases stability using amino acids which are cationic polymers.

In one embodiment of the present invention, the hydrophilic solvent included in the nanoliposomes containing horse oil and placenta extract includes distilled water, purified water, demineralized water, and ethanol aqueous solution, but is not limited thereto.

In one embodiment of the invention, the amino acid that adds water solubility to the nanoliposomes containing horse oil and placenta extract may be selected from the group of essential amino acids consisting of arginine, alanine, phenylalanine, lysine, and histidine.

In one embodiment of the present invention, the emulsifier used in the preparation of the nanoliposomes may be lecithin, preferably soybean lecithin.

In the water-soluble nanoliposome according to one embodiment of the present invention, the amino acid is used as a very useful material that greatly increases the solubility of the liposome in water and maintains and protects the oil phase from the water phase, such as alanine, phenylalanine, arginine, and the like. Any essential amino acid can be selected and used.

In one embodiment of the present invention, the formation of nanoliposomes is preferably carried out at a low temperature of 0 ℃ to 4 ℃.

1 is a layered schematic diagram of nanoliposomes containing horse oil and placenta extract according to an embodiment of the present invention, Figure 2 is a nano liposome containing horse oil and placenta extract according to an embodiment of the present invention purified water This is a picture showing the dissolved state.

As shown in Figure 1, the structure of the nanoliposome containing the horse oil and horse placenta extract according to an embodiment of the present invention is not a complex that formed a chemical bond, the nanoliposome in a state that the placenta extract is enclosed in the cluster dextrin It is the innermost layer of, followed by a layered layer of horse oil and lecithin that forms a physical bond with amino acids. In addition, in Figure 2, it can be seen that the nanoliposomes of the present invention has water solubility using amino acids to improve water solubility.

In one embodiment of the invention, the skin whitening composition comprising nanoliposomes containing horse oil and placenta extract is from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps and sprays The formulation may be selected. For example, the composition for skin whitening according to one embodiment of the present invention is a softening longevity (skin), astringent longevity, nourishing longevity, nutrition cream, massage cream, essence, pack, skin adhesive patch and skin adhesive gel, etc. It can be prepared in various formulations of cosmetics. Skin whitening composition comprising nanoliposomes containing horse oil and placenta extract according to an embodiment of the present invention can also be used as an external skin ointment.

The present invention also provides a method for producing a water-soluble nanoliposome containing horse oil and horse placenta extract comprising the step of mixing the horse oil and horse placenta extract with an emulsifier, amino acids and cluster dextrin to emulsify the water.

In one embodiment of the invention, the method for producing a water-soluble nanoliposome containing horse oil and placenta extract

Dissolving the placenta extract in a hydrophilic solvent,

Adding cluster dextrin to the solution and warming to 50-60 ° C.,

Cooling the warmed solution to 0-4 ° C.,

Slowly adding horse oil and an emulsifier to the solution under stirring, and

Adding amino acids and stirring while maintaining at 0-4 ° C. to form water-soluble nanoliposomes.

In one embodiment of the present invention, the method for preparing a water-soluble nanoliposome may further comprise lyophilizing the formed water-soluble nanoliposome.

In one embodiment of the present invention, horse oil and horse placenta extract used in the method for producing a water-soluble nanoliposome is prepared according to the methods described in Korean Patent Application Publication Nos. 2008-01000313 and 2008-0103490 filed by the present applicant Can be.

In one embodiment of the present invention, the step of cooling the warmed solution comprising the placenta extract and the cluster dextrin to 0-4 ° C. may be performed at a cooling rate of 0.2-0.5 ° C./min.

In one embodiment of the present invention, the placenta extract may be dissolved in a hydrophilic solvent, including but not limited to distilled water, purified water and demineralized water.

In one embodiment of the invention, the emulsifier used for the preparation of water soluble nanoliposomes may be phospholipids and the amino acids may be arginine, phenylalanine, valine, leucine, isoleucine, methionine, threonine, lysine, tryptophan and It may be an essential amino acid selected from the group consisting of histidine.

Water-soluble nanoliposomes comprising horse oil and horse placenta extract according to one embodiment of the present invention may be prepared as follows.

First, 10-50 g of placenta extract powder is completely dissolved at room temperature in 1 kg of water (tertiary distilled water). Thereafter, 50 to 250 g of cluster dextrin was added, mixed at 50 to 60 ° C, and ultra-precision controlled cooling was performed at a cooling rate of 0.2 to 0.5 ° C / min to 4 ° C. After the crystallization is completed, 20 to 100 g of horse oil (palmitooleic acid content 8%) containing palmitooleic acid and 3 to 4 g of soy lecithin are slowly injected and stirred at a speed of 5000 to 8000 rpm. Thereafter, 10 to 50 g of amino acid was added thereto and maintained at 4 ° C. to prepare a nanoliposome by stirring at a speed of 5000 rpm or more. after Depending on the ease of use, this liquid can be prepared into a water-soluble powder through lyophilization.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, an Example is for illustrating this invention and this invention is not limited by these.

The composition for skin whitening of the present invention contains horse oil and horse placenta extract in nanoliposomes to prevent the constipation of horse oil and placenta, which has been a problem in the past, to improve stability, and there is no skin irritation even at high concentrations, and melanin synthesis. By suppressing it brings an excellent skin whitening effect.

Example 1 Preparation of Horse Placenta Extract and Purification of Horse Oil

(1) Preparation of Horse Placenta Extract Using Enzyme

Frozen 1.5 kg of placenta was thawed and ground using a civilian machine. The ground placenta was placed in an agitator, washed until the blood was removed, and then filtered and dehydrated. 9 g of papain and 3 kg of distilled water were added to 1.2 kg of the dehydrated placenta and reacted at 60 ° C. for 5 hours to completely dissolve the placenta in water. Then, 3 g of papain was further added to the decomposed placental solution for complete decomposition, followed by stirring at 70 ° C. for 2 hours. After that, complete protein hydrolysis was confirmed through burette reaction and trichloroacetic acid reaction, and 30 g of activated carbon was added for color and odor suitable for food or cosmetic raw materials, and the mixture was stirred in a stirrer with a vacuum pressure of 0.2 atm and a reaction temperature of 70 ° C. Stir for 30 minutes to remove color and odor. The solution was then filtered to remove activated carbon. At this time, the primary filtration with a 2 μm filter, the secondary filtration with a 0.45 μm filter, the third filtration using a 0.2 μm filter to obtain a placental extract.

(2) refining horse oil

100 g of urea was added to the reactor for 1 kg of unrefined horse oil and reacted at room temperature at a constant stirring speed. Filtration was performed after 1 hour after observing the phenomenon that the surface of the urea expanded as the reaction proceeded. Activated carbon and activated clay were mixed in the obtained filtrate at a ratio of about 2: 8, and 50 g was added to the filtrate, and the reaction was carried out at a vacuum pressure of 200 mmHg and a reaction temperature of 75 ° C. for 2 hours. After the reaction, activated carbon and activated clay were removed through a filtration process. The purified horse oil thus obtained was 825 g. The purified horse oil was cooled to room temperature under a cooling rate of 0.2 degrees / minute and a stirring speed of 0 rpm, that is, stationary crystals. In this process, white crystals were precipitated and filtered after cooling to room temperature. 180 g of water was added to the filtrate in order to deodorize the horse oil separated and purified to a high purity obtained after filtration, and thin film evaporation was performed while stirring. Thin film evaporation was carried out at a feed rate of 0.5 L / hr at 60 ° C. and 0.05 atm to completely remove volatile odor and water.

Example 2 Preparation of Nanoliposomes Containing Horse Oil and Placenta Extract

10 to 50 g of placental placenta extract powder prepared by the method of Example 1 was completely dissolved in 1 kg of water (tertiary distilled water) at room temperature. Thereafter, 50 to 250 g of cluster dextrin was added, mixed at 50 to 60 ° C., and ultra-precision controlled cooling was performed at a cooling rate of 0.2 to 0.5 ° C./min to 4 ° C. When crystallization was completed, 20 to 100 g of horse oil (palmitooleic acid content 8%) containing palmitooleic acid and 3 to 4 g of soy lecithin were slowly injected and stirred at a speed of 5000 to 8000 rpm. Thereafter, 10 to 50 g of amino acids selected from the group consisting of arginine, alanine, phenylalanine, lysine and histitin were added and stirred at a speed of 5000 rpm or more while maintaining 4 ° C to prepare nanoliposomes containing horse oil and placenta extract. After liposomes are prepared, this liquid can be prepared into a water-soluble powder through lyophilization according to the convenience of use.

On the other hand, except for the step of dissolving the placenta extract was carried out a method for producing a nanoliposome containing the horse oil and horse placenta extract as described above, to prepare horse oil-nanoliposomes containing horse oil.

Example 3. Melanin synthesis inhibition experiment in melanocytes

Inhibitory effect of melanin synthesis of nanoliposomes containing horse oil and placenta extract of Example 2 (hereinafter referred to as horse oil-placental extract nanoliposomes) using Mel-Ab melanin cells, pigment cells derived from C57BL / 6 mice That is, the whitening effect was observed. Inhibition of melanin production of horse placenta extract, horse oil-nanoliposomes and horse oil-placenta extract nanoliposomes of Example 2 were carried out at concentrations of 10, 20 and 50 μM, respectively. For melanin synthesis inhibition experiment, 10% fetal calf serum, 100 nM 12-O-tetradecanoylphorbol-13-acetate and 1 nM cholera toxin under 37 ° C., 5% CO ₂ conditions Mel-Ab cells were cultured in Dulbecco's Modified Eagle's Media (DMEM). The cultured cells were detached with 0.25% trypsin-EDTA and seeded with a concentration of 10 ⁵ (cells / well) in a 6-well dish, and the placenta extract, horse oil- Nanoliposomes and horse oil-placental extract nanoliposomes were added at concentrations of 0 μM, 10 μM, 20 μM and 50 μM, respectively, and cultured for 4 days. The cultured cells were collected to remove the culture solution, washed with PBS (phosphate buffered solution), treated with 1N sodium hydroxide to dissolve the melanin, and absorbance was measured at 400 nm with an ELISA reader. In addition, the cells without the horse oil-placental placenta extract nanoliposomes of Example 2 were tested as the control method in the same manner as described above. The inhibition of melanogenesis was calculated using a standard curve of melanin synthesis concentration (5 μg / ml to 300 μg / ml) and the results are shown in the table below.

Melanogenesis inhibitory rate = (C-A) / C × 100 (%)

Wherein C is the melanin concentration (melanin μg / protein μg) of the control sample and A is the melanin concentration (melanin μg / protein μg) of the test sample.

table. Inhibitory Effect of Horse Oil-Placenta Extract Nanoliposomes on Melanin Production

density Placenta Extract
(%) Horse oil-nanoliposomes
(%) Horse oil-Placenta Extract Nanoliposomes (%) 0 μM 0 0 0 10 μM 14.2 0 29.9 20 μM 19.9 5 45.1 50 μM 43.1 12.5 71.0

As shown in the table, horse oil-placental placenta extract nanoliposomes showed an excellent melanogenesis inhibitory effect compared to horse placenta extract alone.

That is, horse oil-placental placenta extract nanoliposomes had an inhibitory effect of 29.9% at a concentration of 10 μM. In particular, compared to horse's placenta extract showed the effect corresponding to 2.1-fold at the same concentration, it was found that the effect is greatly increased by 2.3 times, 1.6 times even in 20 μM, 50 μM respectively.

In addition, Mel-Ab melanin incubated in DMEM with 10% fetal bovine serum, 100 nM 12-O-tetradecanoylphorball-13-acetate and 1 nM cholera toxin under 37 ° C. and 5% CO ₂ conditions The cells and Mel-Ab melanin cells treated with horse oil-placental extract nanoliposome prepared in Example 2 were observed under a microscope, and the results are shown in FIG. 3. As shown in FIG. 3, it was observed that black melanin of Mel-Ab melanin cells normally cultured under normal conditions strongly decreased concentration-dependently in Mel-Ab melanocytes treated with horse oil-placental placenta extract nanoliposomes of the present invention. It became. From these results, it was found that horse oil-placental extract nanoliposomes inhibit melanin synthesis more strongly in melanocytes than horse placenta extract.

Example 4 Stability Test of Nanoliposomes Containing Horse Oil and Placenta Extracts

Essences containing horse oil-placental placenta extract nanoliposomes (1.1% = 0.1% placenta extract content) and placenta placenta extract (0.1%) were prepared according to the method of Example 9 below, and then, respectively, at room temperature and 45 ° C. It was left at ℃ and observed the change in color over time, the results are shown in FIG. As shown in FIG. 4, the observation result for a total period of 30 days showed that horse oil-placental placenta extract nanoliposomes have increased stability by slowing browning rate compared to horse placenta extract.

Example 5 Skin Irritation Test of Nanoliposomes Containing Horse Oil and Placenta Extract

Essences containing horse oil-placental placenta extract nanoliposomes (1.1% = 0.1% in placenta extract content) and horse placenta extract (0.1%) were prepared according to the method of Example 9 below, and then volunteered for 20 volunteers. The skin patch test was performed. Ten horse oil-placental placenta extract nanoliposome applicators had no irritant response in all, whereas the placenta placenta extract applicator experienced mild irritation and erythema in one of ten patients and the results are shown in FIG. 5. Therefore, it can be seen that the horse oil-placental placenta extract nanoliposomes of the present application had a significant decrease in stimulation compared to the horse placenta extract.

Example 6. Preparation of flexible skin lotion (skin) comprising nanoliposomes containing horse oil and placenta extract

According to a conventional method, 1% by weight of horse oil-placenta extract nanoliposome of Example 2, 3% by weight of glycerol, 5% by weight of ethanol, 2% by weight of 1,3-butylene glycol, and the remaining amount of purified water Mixing cosmetics were prepared by mixing.

Example 7. Preparation of astringent lotion containing nanoliposomes containing horse oil and horse placenta extract

According to a conventional method, 1% by weight of horse oil-placenta extract nanoliposome of Example 2, 4% by weight of beeswax, 4% by weight of liquid paraffin, 4% by weight of squalene, 3% by weight of glycerin, trace amounts of preservatives, and flavors, and The remaining amount of purified water was mixed to prepare astringent cosmetic water.

Example 8 Preparation of Nutritional Cream Comprising Nanoliposomes Containing Horse Oil and Placenta Extract

According to a conventional method, 1% by weight of horse oil-placenta extract nanoliposome of Example 2, 6% by weight of 1,3-butylene glycol, 4% by weight of glycerin, 5% by weight of liquid paraffin, 3% by weight of squalene, poly A nourishing cream was prepared by mixing 1.5 wt% of Solvate 60 and the remaining amount of purified water.

Example 9 Preparation of Essence Containing Nanoliposomes Containing Horse Oil and Placenta Extract

According to a conventional method, the horse oil-placental placenta extract nanoliposome 1% by weight, propylene glycol 2% by weight, glycerin 4% by weight, ethanol 7% by weight, preservatives, flavor, and purified water balance Essences containing nanoliposomes were prepared.

Example 10 Preparation of a Pack Containing Nanoliposomes Containing Horse Oil and Placenta Extract

According to a conventional method, 1% by weight of horse oil-placenta extract nanoliposome of Example 2, 6% by weight of propylene glycol, 4% by weight of glycerin, 2% by weight of beeswax, 30% by weight of liquid paraffin, trace amounts of preservatives, And a residual amount of purified water was mixed to prepare a pack containing nanoliposomes.

Figure 1 shows a schematic diagram of the layered structure of the nano liposome containing horse oil and placenta extract according to an embodiment of the present invention.

Figure 2 shows the horse oil and placenta extract in accordance with an embodiment of the present invention dissolved in purified water.

Figure 3 is a microscopic observation of Mel-Ab melanocytes treated with horse placenta extract (I), nanoliposomes (II) containing horse oil and horse placenta extracts according to one embodiment of the present invention, and horse oil nanoliposomes, respectively Shows.

Figure 4 shows the time-lapse stability of the essence containing the placenta extract (A) and nanoliposome (B) containing horse oil and horse placenta extract according to an embodiment of the present invention as an active ingredient, respectively. 4 shows the browning degree of A and B when stored at room temperature and 45 ° C. for 30 days, respectively.

Figure 5 shows the skin irritation test results of the essence containing the placenta extract (A) and nanoliposomes (B) containing horse oil and horse placenta extract according to an embodiment of the present invention as an active ingredient, respectively. 5 shows the irritation and erythema occurring at the site of application (A) before and after application of (A) and (B) for 48 hours.

Claims (12)

Skin whitening composition comprising a nanoliposome containing horse oil and placenta extract as an active ingredient. The composition of claim 1, wherein the horse oil is contained in 2 to 10% by weight based on the total weight of the nanoliposomes. According to claim 1, The placenta extract is a composition for skin whitening is contained in 1 to 5% by weight relative to the total weight of nanoliposomes. The skin whitening composition of claim 1, wherein the nanoliposome is contained in an amount of 0.001 to 10 wt% based on the total weight of the skin whitening composition. The composition of claim 1, wherein the nanoliposomes comprise emulsifiers, cluster dextrins, and hydrophilic solvents. The composition of claim 5, wherein the nanoliposomes are water-soluble nanoliposomes further comprising amino acids. The composition of claim 6, wherein the amino acid is selected from the group consisting of arginine, alanine, phenylalanine, lysine, and histidine. The composition for skin whitening according to claim 5, wherein the emulsifier is lecithin. The composition of claim 1, wherein the composition for skin whitening has a formulation selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps and sprays. Composition for skin whitening. Dissolving the placenta extract in a hydrophilic solvent, Adding cluster dextrin to the solution and warming to 50-60 ° C., Cooling the warmed solution to 0-4 ° C., Slowly adding horse oil and an emulsifier to the solution under stirring, and A method for preparing a water-soluble nanoliposomes containing horse oil and placenta extract, comprising the step of adding amino acids and stirring while maintaining at 0 to 4 ℃ to form a water-soluble nanoliposomes. The method of claim 10, wherein the step of cooling the warmed solution comprising placenta extract and cluster dextrin to 0-4 ° C. is performed at a cooling rate of 0.2-0.5 ° C./min. The method of claim 10, wherein the emulsifier is lecithin.

Images