KR20100094119A - Producing method for yolk powder having a immuneantibody of duck disease and yolk powder using the same - Google Patents

Abstract

PURPOSE: A producing method for yolk powder with an immune antibody for duck disease, and the yolk powder are provided to prevent and cure main disease of a duck. CONSTITUTION: A producing method for yolk powder with an immune antibody for duck disease comprises the following steps: preparing each antibody for each different infectious disease capable of infecting a duck; mixing the antibodies with an oil immunoadjuvant to form an immune forming antigen; injecting the immune forming antigen to a laying hen; collecting eggs from the laying hen; and separating egg yolk, and producing powder with the egg yolk.

Description

Producing Method For Yolk Powder Having A Immuneantibody Of Duck Disease and Yolk Powder Using The Same}

The present invention relates to a composition having a therapeutic and prophylactic effect on duck disease, and in particular, to a method for producing egg yolk powder containing an immune antibody against duck disease, and to an egg yolk powder containing the above-described immune antibody. More specifically, an antigen for duck disease is prepared as an immunologic agent and inoculated in a laying hen, and egg yolk obtained from the egg (egg) is used to provide egg yolk powder useful for duck disease.

In general, animals are immune from being recovered after being infected by a pathogen in order to protect themselves from various diseases. In addition, in order to have an immune function without suffering from diseases, a prophylactic agent may be prepared by artificially inoculating a specific pathogen, and immunization may be obtained by artificially inoculating, or similar effects may be obtained by injecting an already-prepared immune substance into the body.

Among them, ducks are exposed to a large number of pathogens from young age due to breeding, and the resistance to disease is low, and many diseases are easily infected. In some younger ages, the lack of maternal transfer antibodies results in weaker resistance to disease, resulting in a marked loss of defense against various diseases.

The main disease sources for ducks include duck viral hepatitis, duck sepsis, E. coli and Salmonella infections. Currently, vaccines, antibiotics, sulfa drugs, etc. for the disease agents are used to treat and prevent duck disease. Doing.

However, there is a problem that the above vaccine, antibiotics, sulfa drugs and the like cannot effectively treat and prevent duck disease early. In addition, many side effects are caused by excessive use of vaccines and antibiotics. Accordingly, a new level of research is urgently needed to treat and prevent duck disease.

The present invention for solving the above problems is to provide a method for producing an egg yolk powder containing an immune antibody against duck disease, and to provide an egg yolk powder containing the above-described immune antibody so as to treat and prevent a major disease of ducks. Purpose.

In particular, the respective antigens for duck's major diseases, duck viral hepatitis, duck sepsis, E. coli and Salmonella infection, are prepared with one immunologic agent and inoculated in laying hens, and egg (egg) obtained from the egg, It is to provide an egg yolk powder having the effect of preventing and treating major diseases at once.

The present invention for achieving the above object, comprising the steps of preparing an antigen for each of a number of different infectious diseases capable of infecting ducks; Preparing an antigen for immunization by mixing the prepared antigens with an oil adjuvant; Inoculating the prepared immune-forming antigen into a laying hen; Collecting eggs from the laying hens; And collecting the yolk from the collected eggs and preparing the yolk as a powder. The method of producing an yolk powder containing an immune antibody against duck disease, comprising: a.

That is, the present invention relates to a method for producing egg yolk powder containing an immune antibody against duck disease and to egg yolk powder according to the present invention. In particular, an antigen for duck disease is prepared as an immunologic agent and inoculated into a laying hen, and the egg obtained from the egg (egg). Egg yolk is used to provide yolk powder useful for duck disease.

The inventors are the main diseases of duck viral hepatitis in ducks: research to develop (Duck virus hepatitis DVH), duck septicemia (Rimeriella anatipestifer), E. coli (Escherichia coli) increases and Salmonella (Salmonellosis) substances in the infection prevention and cure As a result, a mixture of a plurality of antigens for the disease and the oil adjuvant, a mineral oil additive, was prepared to prepare an immunologic agent, and it was found that the powder of egg yolk obtained by inoculating it in laying hens was effective for the prevention and treatment of the disease. Was completed.

The present invention is based on the egg yolk (Ylok) obtained from chickens immunized with the antigen of duck infectious disease, thereby obtaining egg yolk powder containing immune antibodies against duck viral hepatitis, duck sepsis, E. coli and Salmonella infection The immune yolk antibody powder may be mixed with an existing feed (feed additive) or fed to the duck as a supplementary feed to prevent and treat infectious diseases of the duck.

In the production method of egg yolk powder according to the present invention, the plurality of different infectious diseases are Duck virus hepatitis (DVH), Rimeriella anatipestifer , Escherichia coli and Salmonella ( Salmonellosis ) is an example of an infection, but the disease is merely an example of a major disease source of ducks, the present invention is not limited thereto and may be equally applicable to all kinds of diseases that can be transmitted or developed in ducks. It will be apparent to those skilled in the art.

The antigen for antigen formation is 8% by weight of duck viral hepatitis antigen, 6% by weight of duck sepsis antigen, 6% by weight of E. coli antigen, 10% by weight of Salmonella infectious antigen and oil adjuvant 70 It is more preferable to prepare by mixing the weight percent, which is the ratio that can be optimally suited for making one immunologic with antigens against other disease agents considering the titer content of duck viral hepatitis It was confirmed that.

In addition, the duck sepsis antigen, E. coli antigen and Salmonella infectious antigen is most preferably an antigen having an OD (Optical density) value of 1.2 at a wavelength of 410 nm with a spectrophotometer for each cultured cells, the present inventors It was found that the optimal number of bacteria was optimal when making antigens for eggplant disease.

Subsequently, in the production method of the egg yolk powder, inoculation of the antigen for immunization into a laying hen can be inoculated three times at three week intervals. That is, the first inoculation, the second inoculation three weeks after that, and the third inoculation three weeks after that, and the multiple inoculation at regular intervals in this way to effectively effect the immune response in laying hens.

In addition, manufacturing the collected egg yolk as a powder may be to dry the moisture of the yolk by a spray method in the form of a disk and a nozzle using a spray dryer. Further, in the method of producing the yolk powder, preparing the sampled yolk powder may rapidly freeze the yolk yolk to a sub-zero temperature, and in a vacuum at a second temperature lower than the first temperature. It is also possible to dry the moisture of egg yolk.

As described above, another embodiment of the present invention may be an egg yolk powder produced by the above-described method for producing yolk powder, containing an immune antibody against a plurality of different infectious diseases. In addition, the present invention is also capable of egg yolk powder containing immuno-antibodies against duck viral hepatitis, duck sepsis, Escherichia coli and Salmonella infection. The yolk powder according to the present invention enables to effectively and simply treat and prevent a number of different diseases associated with ducks.

Specific details of other embodiments are included in the detailed description and the drawings.

The present invention as described above provides a method for producing yolk powder containing an immune antibody against duck disease and an egg yolk powder comprising the above-described immuno-antibody, thereby effectively and simply treating and preventing the main diseases of ducks.

In particular, the respective antigens for duck's major diseases, duck viral hepatitis, duck sepsis, E. coli and Salmonella infection, are prepared with one immunologic agent and inoculated in laying hens, and egg (egg) obtained from the egg, It can provide yolk powder having the effect of preventing and treating a variety of major diseases.

Furthermore, the yolk powder according to the present invention can be simply mixed and supplied to the general feed of ducks, and thus can be a new level of duck disease prevention and treatment that can replace the use of conventional vaccines, antibiotics and sulfa agents.

Hereinafter, one preferred embodiment of the present invention will be described in detail with reference to the accompanying drawings. The invention may be better understood by the following examples, which are intended for purposes of illustration of the invention and are not intended to limit the scope of protection defined by the appended claims.

Example 1 Production of Egg Yolk Powder Containing Immune Antibody for Duck Disease

Example 1-1 Antigen Preparation of Duck Disease Cause Agents

(1) Duck Viral Hepatitis (DVH) Antigen Preparation

end. Virus propagation

After lyophilization, duck hepatitis virus (DRL62) stored at −80 ° C. was inoculated with 0.2 ml of the intestinal ganglia of 10-day-old Specific pathogen free (SPF) embryonated eggs and incubated at 37 ° C. for 3-4 days.

I. Virus poisoning

As above, while incubating at 37 ° C. every day, at the time of dying, the trunk was collected except the head of the chick.

All. Inactivation of the virus

The virus was recovered by emulsifying the trunk of the chick embryo collected as above with 20% Dulbecco's Phosphate buffered solution (PBS). Formalin was added to the recovered virus at 0.1%, inactivated at room temperature for 24 hours, and then centrifuged at 5,000 rpm for 20 minutes at high speed, and the resultant was used as an infected virus.

la. Virus titer test

In order to test the titer of virus contaminating the torso of infected embryos before inactivation, the virus was diluted by 10 to 10 ^1.0 to ^8.0 and inoculated 0.1 ml in the urinary membrane cavity of 10-day-old SPF embryonated eggs at 37 ° C. The mortality was measured while culturing for 5 days at. In the case of surviving chick embryos, the presence of viral infection was checked by considering viral edema and atrophy of duck viral hepatitis-specific lesions, and necrotic liver formation. Liver embryos were infected with EID ₅₀ (50). The virus titer was 10 ^7.5 EID ₅₀ per ml as calculated by% Egg infective dose.

(2) Duck Sepsis Antigen Preparation

end. Spawn culture

After freeze drying, Rimerella anatifestifer, a duck sepsis, stored at -80 ° C (Rimeriella anatipestiferThe strain was transplanted into Sheep blood agar medium and incubated at 37 ° C for 24 hours.

I. Present culture

A typical single colony of duck sepsis was selected from the culture medium, which was transplanted into a medium prepared by adding Sodium bicarbonate to Brain heart infusion broth medium, followed by shaking culture at 37 ° C. for 30 hours.

All. Sterilization and Processing

0.3% formalin was added to the infected culture medium shaken at 37 ° C for 30 hours, inactivated, and concentrated. Then, the concentrated culture cells were centrifuged at 5,000 rpm for 30 minutes, and cells were collected by Dulbecco's Phosphate buffered solution (PBS). After the collected cells were washed twice, the OD (Optical density) value was measured at a wavelength of 410 nm using a spectrophotometer and then stored in a cold room (5 ° C.).

(3) Escherichia coli ( E. coli A) antigen production

end. Spawn culture

After lyophilization, strain 078 stored at −80 ° C. was transplanted into Sheep blood agar medium, and cultured at 37 ° C. for 20 hours.

I. Present culture

A typical single colony of Escherichia coli was selected from the cultured medium, transplanted into Brain heart infusion broth medium, and cultured with shaking at 37 ° C for 18 hours.

All. Sterilization and Processing

Incubated with 0.3% formalin for 18 hours at 37 ℃ shake culture infected culture was inactivated and concentrated, and the cells were collected by centrifugation of the concentrated culture cells at 5,000 rpm for 30 minutes, collected by Dulbecco's Phosphate buffered solution (PBS) After washing the cells twice, the OD (Optical density) value was measured at a wavelength of 410nm by using a spectrophotometer and stored in a cold room (5 ℃).

(4) Salmonella ( Salmonella Antigen production

end. Spawn culture

After lyophilization , S. pullorum, S. gallinarium, and S. enteritidis strains stored at −80 ° C. were transplanted into Sheep blood agar medium and incubated at 37 ° C. for 20 hours.

I. Present culture

In the culture medium, typical single colonies of S. pullorum, S. gallinarium and S. enteritidis were selected and transplanted into Brain heart infusion broth medium, respectively, and cultured at 37 ° C. for 20 hours.

All. Sterilization and Processing

Inactivated by adding 0.3% formalin to each of the infected culture medium shaken at 37 ℃ for 18 hours, concentrated, and then the concentrated culture cells were centrifuged at 5,000 rpm for 30 minutes to collect the cells and Dulbecco's Phosphate buffered solution ) Were collected twice and then stored in a cold room (5 ° C) after each measurement of OD (Optical density) at a wavelength of 410 nm using a spectrophotometer.

Dulbecco's Phosphate buffered solution (PBS) used to prepare the antigen was prepared by including 8.00 g of NaCl, 0.20 g of KCl, 1.15 g of Na ₂ HPO ₄ , and 0.20 g of KH ₂ PO ₄ in 1,000 ml of distilled water, and 121 ° C. Autoclaved for 15 minutes at

Example 1-2 Antigen Production for Immunogenesis

Antigens for immunization were produced using the antigens of the disease agent prepared according to Example 1-1. In other words, 8% antigen of duck viral hepatitis 10 ^7.5 ELD ₅₀ /, duck sepsis, Escherichia coli and Salmonella were adjusted to the OD value 1.2 by measuring the OD (Optical density) value at a wavelength of 410 nm using a spectrophotometer. Mix 6% of the duck sepsis antigen, 6% of the E. coli antigen, and 10% of the Salmonella antigen, add 70% of the oil adjuvant (Montnide ISA70), mix well, and then use the Homogenizer for 5 minutes at 15,000 rpm. Produced.

Example 1-3: Inoculation of Immunogenic Antigens into Laying Hens

Immunogenic antigens produced according to Example 1-2 were firstly inoculated into the breast muscle at 0.8 ml per horse in 19-week-old laying hens (chicken), the second inoculation was 3 weeks after the first inoculation, and the third inoculation was 2 Three weeks after the primary inoculation, the muscles were inoculated respectively, and then the vaccinations were further inoculated every three months.

Example 1-4: Egg Collection and Preparation of Egg Yolk Powder

After 2 weeks after the third inoculation of the immunogenic antigen according to Example 1-3, the eggs with the immune antibody were hygienically collected, and then 150 ppm of sodium hypochlorite was sprayed onto the eggs to clean the eggs, and then in the drying room. The surface of the was completely dried with moisture.

Eggs were dried to obtain egg yolks, and the yolks were collected by spray drying or freeze drying. That is, the spray drying process is a method of drying the moisture of the yolk sac, which is immunized according to the spray method in the form of a disk and a nozzle using a spray dryer, which is dried and processed into a powder having a moisture content of less than 10%. In addition, the freeze-drying process is a method of drying the moisture of the egg yolk immunized under vacuum when the frozen egg yolk is rapidly frozen to -40 ° C and the freezer dryer temperature is -80 ° C. The powder was dried to less than 10% and processed.

Example 1-5: Immune Antibody Extraction in Egg Yolk

On the other hand, the present inventors extracted the antibody in egg yolk of the eggs collected according to Example 1-4. That is, eggs are collected and washed and disinfected with 150 ppm sodium hypochlorite solution, and then the egg yolk emulsion: pH 4.0 is mixed with 5: 1 water and stored in a cold room at 4 ° C. for 24 hours. The antibody contained in egg yolk was extracted by centrifugation and purification at 8,000 rpm for 20 minutes.

Experimental Example 1 Antibodies to Antigen Experiment

In order to determine the antibody value against the pathogens formed in egg yolk after inoculating the laying hens, the eggs (duck viral hepatitis, duck sepsis, Escherichia coli and Salmonella infection antigens collected in accordance with Example 1-4 were added to the laying hens). After two weeks of inoculation, the eggs of immunized laying hens were obtained two weeks later, and the antibody titers of the pathogens of the antibodies formed in egg yolk were examined by neutralization assay and enzyme-linked immunosorbent assay (ELISA).

Experimental Example 1-1: neutralization test method

     Neutralization test is to determine how many antibodies to the virus by changing the amount of virus in egg yolk using a fetus. 0.4 ml of the extract extracted from the egg yolk according to the present invention was added to 0.4 ml of the diluted solution diluted from 1:10 to 1: 2,048 times as a test group, and then neutralized for 1 hour at 37 ° C, followed by development of 10 days of age. Eggs were inoculated with 0.1 ml into the urinary membrane of each of the five developing eggs by dilution factor. For control, the mortality was measured by incubating at 37 ° C for 7 days with five embryonated eggs.The embryos of surviving embryonated eggs considered lesions such as chick edema and atrophy, and liver necrosis, which are specific to duck viral hepatitis. The presence of virus infection was confirmed and measured by neutralization index (meutralization index).

As a result, the neutralizing antibody titer for duck viral hepatitis contained in the yolk sac of immunized eggs was 1,280 fold, and the control antibody showed 10-fold or less antibody titer (Table 1).

Table 1: Neutralizing Antibodies for Duck Viral Hepatitis

division Neutralizing antibody Experimental egg yolk powder 1,280 times Control egg yolk powder 10 times or less

Experimental Example 1-2: enzyme immunoassay (ELISA)

     The basic principle of enzyme immunoassay is to use enzyme digestion to check the binding state of antigens in cells and tissues and specific antibodies specific for the antigens. It is a method of demonstrating binding of an antibody by making it appear. In other words, it is a method of confirming the presence of antigen, antibody, etc. by reacting the antigen adsorbed in the microplate or the antigen, antibody or labeling substance of each antibody to be combined, and reading the color development in the microplate well using an ELISA reder.

First, each cell was harvested by centrifugation of the cells cultured with duck sepsis, E. coli and Salmonella at 20,000 rpm and 4 ° C. for 30 minutes, and the cells were washed three times with sterile PBS. Aliquoted to 5 mg / mL and stored at -70 ° C.

Then, using the ELISA microplate prepared by diluting the antigen with a coting buffer and then dispensing 100 μl per plate well, the color development of the yolk powder and the yolk powder of Experimental Example 1-2 were measured. , Duck sepsis showed antibody titer of 1,280 times or more, Escherichia coli showed 2,560 times, and Salmonella infection showed 1,280 ~ 2,560 times or more, respectively. Table 2).

Table 2: ELISA Antibodies to Sepsis, Escherichia Coli, and Salmonella

By strain ELISA antibodies Experimental egg yolk powder Control egg yolk powder Duck sepsis 1,280 times 10 times or less Escherichia coli 2,560 times 10 times or less S. pullorum 1,280 times 10 times or less S. gallinarium 1,280 times 10 times or less S. enteritidis 2,560 times 10 times or less

Experimental Example 2 Test of efficacy and effect of egg yolk powder on disease agent

Experimental Example 2-1: Efficacy test on duck viral infection

In order to confirm whether the yolk powder according to the present invention can increase the infection prevention and treatment effect against the antigen of duck viral infection, 0.5% of the yolk antibody powder prepared according to Example 1-4 in the general feed as an experimental group Added mixed feed was prepared, and normal feed was prepared as a control. Each prepared feed was fed to 100 three-day-old ducks twice daily for 14 days and clinically observed.

As a result, as shown in Table 3, among the 100 experimental groups, 28 ducks that suddenly got down and collapsed to one side and showed neurological symptoms such as shaking head and neck, struggling, coughing and sneezing, 22, green Clinical symptoms including 19 diarrhea and 26 secretions from eyes and nasal passages were observed. Seventeen died due to multiple disease infections. In the control group, 51 ducks suddenly fell down and fell to one side, shaking head and neck, struggling, 42 coughs and sneezes, 37 green diarrhea, and secretions from the eyes and nasal passages. The clinical symptoms worsened compared to the experimental group including 46 animals, and 32 of them died due to multiple disease infections.

Table 3: Efficacy test results for duck viral infection

division
duck
Payday
Clinical observation
Neurological symptoms cough,
Wit Green stools Discharge
(Eyes, nasal passages) Our company Experimental group 100 14 days 28/100 ^* 22/100 19/100 26/100 17/100 Control 100 Non-payment 51/100 42/100 37/100 46/100 32/100

* Symptoms / Number of Tests

In addition, the ducks that showed clinical symptoms in the experimental group showed a rapid recovery state, and confirmed the effect of the yolk powder according to the present invention. As a result of autopsy of some dead ducks, fibrogenic exudate appeared in the liver and heart. It could be confirmed.

Experimental Example 2-2: Effect experiment on duck sepsis

The experimental group was prepared by feeding the mixed feed obtained by adding the yolk antibody powder 0.5% prepared according to Example 1-4 to the general feed twice daily for 14 days to 300 healthy ducks of 3 days of age.

Then, the duck sepsis strain was transplanted into Sheep blood agar medium and incubated at 37 ° C. for 24 hours, and then a single colony of duck sepsis was selected and transplanted into a medium prepared by adding Sodium bicarbonate to Brain heart infusion broth medium. This was incubated at 37 ° C. for 24 hours. The infected culture solution was centrifuged at 5,000 rpm for 30 minutes to collect the cells, and the cells collected with PBS (Phosphate buffered solution) were washed, and then the optical density (OD) was adjusted to 1.2 at 410 nm using a spectrophotometer. 1.0 ml each of the prepared bacteria was prepared.

Thus prepared bacteria were inoculated into the muscles of 100 of the 300 ducks in the experimental group, and 14 days of observation showed 28 clinical symptoms of typical sepsis and the remaining 72 survived.

Experimental Example 2-3: Effect experiment on E. coli

The experimental group was prepared by feeding the mixed feed obtained by adding the yolk antibody powder 0.5% prepared according to Example 1-4 to the general feed twice daily for 14 days to 300 healthy ducks.

Then, E. coli strains were transplanted into Sheep blood agar medium and incubated at 37 ° C. for 20 hours, and a single colony of E. coli was selected and transplanted into Brain heart infusion broth medium, which was incubated at 37 ° C. for 24 hours. The infected culture solution was centrifuged at 5,000 rpm for 30 minutes to collect the cells, and the cells collected with PBS (Phosphate buffered solution) were washed, and then the optical density (OD) was adjusted to 1.2 at 410 nm using a spectrophotometer. 1.0 ml each of the prepared bacteria was prepared.

The bacteria thus prepared were inoculated into the muscles of 100 of the 300 ducks of the experimental group, and after 14 days of observation, 23 showed clinical symptoms of typical sepsis and the remaining 77 survived.

Experimental Example 2-4: Effect test on Salmonella

The experimental group was prepared by feeding the mixed feed obtained by adding the yolk antibody powder 0.5% prepared according to Example 1-4 to the general feed twice daily for 14 days to 300 healthy ducks of 3 days of age.

And Salmonella strains Salmonella pullorum, Salmonella gallinarium, Salmonella enteritidis strains were respectively transplanted in Sheep blood agar medium and incubated at 37 ° C. for 20 hours, respectively, and the typical single colonies of the bacteria were selected and transplanted into Brain heart infusion broth medium, respectively. This was incubated at 37 ° C. for 18 hours each. The infected culture medium was mixed and centrifuged at 5,000 rpm for 30 minutes, and the cells collected with PBS (Phosphate buffered solution) were washed, and the OD (Optical density) was adjusted to 1.2 at 410 nm using a spectrophotometer. 1.0 ml each was prepared.

The bacteria thus prepared were inoculated into the muscles of 100 of the 300 ducks of the experimental group, and after 14 days of observation, 25 showed clinical symptoms of typical sepsis and the remaining 75 survived.

As a result of Experimental Example 2-2, 2-3, 2-4, yolk powder according to the present invention showed excellent clinical effects of 72.0% against sepsis, 77.0% in E. coli, 75.0% in Salmonella, respectively. In addition, 7 days after challenge, the pathogen showed clinical symptoms and died in severe cases, and the clinical symptoms showed that fibrotic exudate appeared in the liver and heart.

On the other hand, while the present invention has been shown and described with respect to certain preferred embodiments, the invention is variously modified and modified without departing from the technical features or fields of the invention provided by the claims below It will be apparent to those skilled in the art that such changes can be made.

Claims (9)

Preparing each antigen for a number of different infectious diseases capable of infecting ducks; Preparing an antigen for immunization by mixing the prepared antigens with an oil adjuvant; Inoculating the prepared immune-forming antigen into a laying hen; Collecting eggs from the laying hens; And, A method of producing egg yolk powder containing an immune antibody against duck disease, comprising the steps of: collecting egg yolk from the collected eggs and preparing the collected yolk into powder. The method of claim 1, wherein the plurality of different infectious diseases, Duck virus hepatitis (DVH), duck sepsis (Rimeriella anatipestifer), E. coli (Escherichia coli) Sickness and Salmonella (SalmonellosisA method of producing egg yolk powder containing an immune antibody against duck disease, characterized in that the disease. The method of claim 2, wherein preparing the immunogen for antigen formation, Duck duck, characterized in that prepared by mixing 8% by weight of the viral hepatitis antigen, 6% by weight of the duck sepsis antigen, 6% by weight of E. coli antigen, 10% by weight Salmonella infectious antigen and 70% by weight of oil adjuvant (oil adjuvant) Method for producing yolk powder containing immune antibodies against diseases. The duck disease according to claim 3, wherein the duck sepsis antigen, E. coli antigen and Salmonella infectious antigen are antigens having an OD (Optical density) of 1.2 at a wavelength of 410 nm with a spectrophotometer for each cultured cell. Method for producing yolk powder containing the immune antibody. The method of claim 1, wherein inoculating the laying hens with the antigen for immunization, Method of producing an egg yolk powder containing an immune antibody for the duck disease, characterized in that three times inoculated with the antigen for immunization three times. The method of claim 1, wherein the prepared egg yolk is prepared as a powder. A method of producing egg yolk powder containing immuno-antibodies to duck disease, characterized in that the drying of the egg yolk moisture in a disk and nozzle type spraying method using a spray dryer. The method of claim 1, wherein the prepared egg yolk is prepared as a powder. The yolk powder of the yolk powder containing immuno-antibodies to duck disease, characterized in that the yolk is rapidly frozen to the first temperature below zero, and the moisture of the yolk is dried in a vacuum at a second temperature lower than the first temperature. Production method. Egg yolk powder produced by the method for producing yolk powder according to any one of claims 1 to 7, containing an immune antibody against a number of different infectious diseases. Egg yolk powder containing immune antibodies against duck viral hepatitis, duck sepsis, Escherichia coli and Salmonella infection.