KR20100088447A - Method and system for performing authentication between authentication material and testing material using online dna digital information - Google Patents

Abstract

PURPOSE: An authentication performing method and a system between test target material and authentication target material using online DNA digital information capable of determining more conveniently and rapidly whether test target material and authentication target material are same materials are provided to use the received identification target material information and enable to generate identification target material dynamically for an inspection device. CONSTITUTION: A management server(10) stores identification target detection material information corresponding to identification target material. A test device(20) inspects whether the test target material and authentication target material include same DNA information. A client device(30) requests the identification target detection material information. The client device requests whether the test target material and authentication target material include same DNA information is checked using the received identification target detection material information.

Description

METHOD AND SYSTEM FOR PERFORMING AUTHENTICATION BETWEEN AUTHENTICATION MATERIAL US TESTING MATERIAL USING ONLINE DNA DIGITAL INFORMATION}

The present invention relates to a method and a system for performing authentication between a substance to be tested and a substance to be authenticated using on-line DNA digital information in a client device coupled to an inspection device and a management server. Receiving the information of the information, requesting the authentication target detection material information stored corresponding to the authentication target substance from the management server using the input information, receiving the authentication target detection substance information from the management server, and receiving the received information. By using the authentication target detection material information, requesting the inspection device to perform a test on whether the test target material and the authentication target material contain the same DNA information is more convenient for authentication between the test target material and the authentication target material. To make sure and do it correctly And to the system.

Recently, many problems have arisen regarding the stability of food. Therefore, the demand of the public to confirm the origin of the food material or genetic modification that can ensure the stability of the food is increasing significantly. However, the information that a general consumer can verify about food is all about the labeling of the food ingredients or the labeling of the origin.

Country of origin and the like marked on the food has a problem that the reliability is low because there is a fear of being modified or hidden by the food provider. In order to accurately verify the stability of the food or the origin of the material, analyzing the DNA information of the actual food would be the most accurate and reliable method.

Conventional techniques for verifying the identity (origin, toxicity, paternity) of a substance by grasping DNA information such as a substance include a method of directly analyzing DNA characteristics in a laboratory and a method of analyzing directly outside a laboratory using a gene chip. And the like.

The first method, which analyzes DNA information in the laboratory, is divided into methods of analyzing and comparing protein DNA components, and making complementary DNA sequences to perform hybridization reactions and analyzing reaction results.

The method of analyzing protein DNA components is a method mainly used in paternity screening, and is a method of analyzing protein DNA components using an electrophoretic device and then comparing the similarities between the two DNA components. The hybridization reaction method is mainly used for disease test or food origin analysis. When a hybridization reaction occurs after making a complementary DNA sequence having a symmetrical linkage structure to a unique sequence of DNA-containing substances, It is a method of verifying that a substance to be tested contains unique DNA information that only the substance exists.

The second method, using a gene chip, is a method of making a small-scale, low-cost portable chip to test a material having a complementary DNA sequence in real time so that the gene can be tested in large quantities from the outside.

Since the first method is a long time and expensive because the DNA to directly identify the characteristics of the laboratory, there is a limit to use for general users, there is a problem that is currently used only for special purposes, such as paternity identification. The second method uses a mass-produced gene chip, so that DNA characterization time is short and relatively inexpensive. However, since the genetic chip analyzes only a few fixed DNA characteristics, only limited information can be detected. There is also a limit to the certification of a wide range of substances. In addition, there is a limit that requires a lot of time and cost to manufacture, distribute, and utilize a new gene chip using the newly discovered DNA characteristic information.

The present invention has been made to solve the above problems, and it is easier and faster to determine whether the material to be tested and the material to be certified are the same material by using DNA information in the client device combined with the test device and the management server. It is an object of the present invention to provide a method and a system capable of doing so.

In addition, the present invention, the client device coupled to the inspection device and the management server receives the information of the authentication target detection material stored in correspondence with the authentication target material in real time from the management server, and using the received authentication target detection material information It is yet another object of the present invention to provide a method and system for requesting to dynamically generate a detection subject to be certified.

In addition, the present invention by digitally storing the authentication target detection material information that can confirm that the authentication target material to the management server connected to the client device via the wired or wireless network, the client device to store the authentication target detection material information corresponding to the desired material It is another object of the present invention to provide a method and apparatus that can be quickly received and used anytime, anywhere.

In order to achieve the above object, the present invention provides a method for performing authentication between a substance to be tested and a substance to be authenticated using online DNA digital information in a client device coupled with an inspection device and a management server, wherein the substance to be authenticated. A first step of receiving the information of; Requesting authentication target detection substance information stored corresponding to the authentication target substance to the management server by using the input information; A third step of receiving the authentication target detection substance information from the management server; And a fourth step of requesting the inspection apparatus to perform a test on whether the test target material and the test target material include the same DNA information using the received authentication target detection material information. The device provides a method for performing authentication between a material to be tested and a material to be authenticated using on-line DNA digital information, which performs a test at the request of the client device and transmits the test result to the client device.

Here, the inspection of whether the test target material and the test target material including the same DNA information performed in the test apparatus may include generating an authentication target detection material using the test target detection material information; Synthesizing the generated DNA detection material and the DNA material extracted from the test material; And analyzing the synthesis result.

Here, the authentication target detection material information may be digital information DNA information.

Here, the DNA information may be a DNA base sequence.

Here, the client device may be one of a computer, a mobile phone or a notebook.

Here, in the third step, the authentication target detection material information may be received through a wired or wireless network.

According to another aspect of the present invention, a system for performing authentication between a substance to be tested and a substance to be authenticated using online DNA digital information, comprising: a management server storing authentication object detection material information corresponding to the substance to be authenticated; An inspection device for inspecting whether the inspection target material and the authentication target material include the same DNA information; And receiving the information of the authentication target substance, requesting authentication target detection substance information stored corresponding to the authentication target substance from the management server using the input information, and receiving the authentication target detection substance information from the management server. An on-line DNA that includes a client device that receives and requests the inspection device to perform a test as to whether or not the inspection target material and the authentication target material contain the same DNA information using the received authentication target detection material information. It provides a system for performing authentication between a substance to be inspected and a substance to be certified using digital information.

Here, the inspection of whether the test target material and the test target material including the same DNA information performed in the test apparatus may include generating an authentication target detection material using the test target detection material information; Synthesizing the generated DNA detection material and the DNA material extracted from the test material; And analyzing the synthesis result.

Here, the inspection device, the authentication target detection material generating unit for generating the authentication target detection material; A DNA extracting unit extracting a DNA material from the test target material; And a synthesis result analyzing unit configured to analyze a synthesis result of the authentication target detection material generated from the authentication target detection material unit and the DNA material extracted from the DNA extracting unit.

Here, the authentication target detection material information may be digital information DNA information.

Here, the DNA information may be a DNA base sequence.

Here, the client device may be one of a computer, a mobile phone or a notebook.

Here, when the client device receives the authentication target detection substance information from the management server, it may be received via a wired or wireless network.

According to the present invention, it is possible to provide a method and system that can more easily and quickly determine whether a substance to be tested and a substance to be certified are the same substance by using DNA information in a client device coupled with the test apparatus and the management server. have.

In addition, according to the present invention, the client device coupled with the inspection apparatus and the management server receives the information of the authentication target detection substance stored corresponding to the authentication target substance from the management server, and uses the received authentication target detection substance information to the inspection apparatus. A method and system may be provided that may request to dynamically generate a detection subject to be certified.

In addition, the present invention by digitizing and storing the authentication target detection material information that can confirm that the authentication target material to the management server connected through the network and the client device, the client device when the authentication target detection material information corresponding to the desired material It can provide a method and apparatus that can be received and used anywhere.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 is a view schematically showing the overall configuration of a system for performing authentication according to the present invention.

Referring to FIG. 1, an authentication performance system using DNA information according to the present invention includes a management server 10, a client device 20, and an inspection device 30.

The management server 10 is a means for storing authentication target detection material information corresponding to the authentication target material. The substance to be certified is a term meaning a substance to be certified. For example, if a piece of meat is to be checked whether or not it is a Korean beef, the "meat piece" is a substance to be inspected, and "Korean beef" is a substance to be certified. . The substance to be authenticated is a term used to mean a substance that can identify the substance to be certified. For example, when the substance to be certified is Hanwoo, the target of detection of complementary DNA sequence information that reacts with specific DNA sequences included in the Korean cattle is detected. Can be specified as a substance. That is, the authentication target detection material is a means for confirming whether or not the test target material is an authentication target material. In this manner, the management server 10 stores information on authentication target detection substances. In this case, the authentication target detection substance information is preferably digitally stored for convenience of management and quickness of retrieval.

The inspection apparatus 10 includes means for inputting a substance to be tested, generates the authentication target detection material, and whether the test target material and the authentication target material include the same DNA information by using the generated authentication target detection material. Means to perform a check on whether or not. Here, the inspection of whether the test target material and the authentication target material contain the same DNA information may include generating an authentication target detection material using the authentication target detection material information, the generated authentication target detection material and the Preferably, the method comprises the steps of synthesizing the DNA material extracted from the test material and analyzing the synthesis result. The inspection apparatus 10 includes an authentication target detection substance generator, a DNA extractor, and a synthesis result analyzer. Detailed configurations of the devices included in the inspection apparatus will be described later with reference to FIG. 3.

The client device 30 receives the information of the authentication target substance, requests the management server 10 to store the authentication target detection substance information corresponding to the authentication target substance by using the input information, and the management server ( 10) receiving the authentication target detection material information, and requests the inspection device 20 to generate an authentication target detection material by using the received authentication target detection material information, the test target material and the authentication target material This is a means of requesting to perform a test as to whether it contains the same DNA information. Since the client device 30 is connected to the management server 10 and the inspection device 20 to transmit and receive information, it is preferable that the client device 30 is a device capable of connecting to a wired or wireless network such as a computer terminal, a mobile phone terminal, or a laptop.

Various types of business can be performed using the authentication performance system according to the present invention. For example, the management server 10 is operated by a national agency as an online server device, and the client device 30 is a home computer by a general user. A terminal, a mobile phone terminal, etc. may be used, and the inspection apparatus 20 may be set so that a user of the computer terminal or the mobile terminal can purchase and connect to the client device 30. The user who purchased the inspection apparatus 20 can obtain the effect of checking the stability of the desired food or substance, the origin, etc. from time to time.

In addition, as well as performing authentication between materials by using the present invention, it can be utilized when authenticating a user. For example, the DNA information (hair DNA information) set differently for each person is registered in the management server 10 according to the present invention, and the user's hair material or the like is used in the inspection device 20 connected to the client device 30. It is also possible to set up to perform user authentication.

2 is a flowchart illustrating a process of performing authentication between a test target material and an authentication target material using DNA information generated in the authentication performance system according to the present invention.

Referring to FIG. 2, first, the management server 10 stores information about an authentication target detection substance (S100). For example, when storing DNA sequencing information, the information on the detection target substance to be authenticated is preferably digitalized and stored. A method of digitally integrating the DNA base sequence will be described later with reference to FIG. 4. In the management server 10, it is preferable to store and input information about an authentication target detection material corresponding to as many authentication target materials as possible.

A user of the client device 30 to determine whether the test target material is the same as the authentication target material inputs information of the authentication target material to the client device 30 (S110). In this case, various methods may be used as a method of inputting information on a material to be authenticated. For example, it may be set to directly input the name of the authentication target, such as "Korean beef". At the same time as inputting the information of the substance to be authenticated into the client device 30, the substance to be inspected is put into the inspection apparatus 20 (S110). As described above, the substance to be inspected means a substance that the client wants to compare with the substance to be authenticated. Injecting the substance to be tested may be performed by, for example, injecting a piece of the substance to be examined, which is assumed to be a domestic cattle, into the inspection apparatus when the substance to be inspected is a Korean cattle.

After the steps S100 to S120, the client device 30 requests the management server 10 to store the authentication target detection material information corresponding to the authentication target material by using the input authentication target material information (S130). For example, if the substance to be authenticated is Hanwoo, the client device 30 may use the management server to detect authentication target material information capable of detecting the Korean cattle stored corresponding to "Hanwoo", that is, a complementary DNA base that reacts with a specific DNA sequence of the Korean cattle. To request sequence information.

The management server 10 receiving the request of the client device 30 retrieves the requested authentication target detection material information from the internal database, and transmits the retrieved authentication target detection material information to the client device 30. In this case, it is preferable that specific DNA sequencing information is digitalized as the detection target material information. The client device 30 that has received the authentication target detection material information from the management server 10 requests the inspection apparatus 20 to perform a test as to whether the authentication target detection material and the test target material contain the same DNA information. (S150 and S160).

After receiving the above request from the client device, the inspection device 20 generates the authentication target detection material using the authentication metabolism detection material generation unit included therein (S170). The inspection apparatus 20 that generates the authentication target detection material 20 extracts a DNA material from the test target material by synthesizing the extracted DNA material and the generated authentication target detection material by synthesizing the extracted DNA material with the same process as the test target material. It is checked whether the DNA information is included (S170 to S190). The inspection apparatus 20 which derives the result of such inspection execution transmits the analysis result back to the client apparatus 30 (S200). The generation process and the authentication process of the authentication target detection substance made by the inspection device 20 will be described later in detail with reference to FIG. 3.

3 is a block diagram illustrating a process of performing authentication between a test target material and a test target material that are performed in a test apparatus.

Referring to FIG. 3, the test apparatus 20 according to the present invention includes an authentication target detection substance generator 21, a DNA extractor 22, and a synthesis result analyzer 23.

The authentication target detection substance generation unit 21 is a means for generating a DNA sequence capable of detecting the authentication target. When receiving the request to perform the inspection from the client device 30, the authentication target detection substance generation unit 21 dynamically generates a corresponding substance. Preferably, the client device 30 transmits information about a digitally-informed authentication target detection substance, and the authentication target detection substance generation unit 21 receives digitally-informed information, and converts the digitally-informed information into an original base sequence. The information is converted into information, and an authentication target detection substance having a structure of the converted base sequence is generated.

The DNA extracting unit 22 is a means for extracting DNA from the test target substance introduced into the test apparatus 20. Extracting DNA from a specific substance corresponds to the conventional art, and thus, a detailed process of extracting DNA from the substance to be tested is omitted.

Authentication is performed by synthesizing the authentication target detection material generated from the authentication target detection material generation unit 21 and the DNA extracted from the DNA extraction unit 22. That is, by synthesizing the DNA sequence material capable of detecting the authentication target and the DNA extracted from the test target using a hybridization reaction or the like to test whether the test target material contains the same DNA information as the authentication target material. will be. Since the reaction occurs when the DNA sequences to be tested have complementary arrangements are also obvious to those skilled in the art, detailed description thereof will be omitted.

The synthesis result analyzing unit 23 is a means for analyzing the result of DNA synthesis made by the configuration of the authentication target detection substance generating unit 21 and the DNA extraction unit 22. That is, as a result of synthesizing the DNA extracted from the authentication target detection material and the test target material, it is determined whether the authentication target material and the test target material contain the same DNA information, and the result is transmitted to the client device 30. The result of the synthesis may mean either the result that the substance to be tested and the substance to be tested contains the same DNA information or the result that the substance to be tested and the substance to be tested does not contain the same DNA information.

4A and 4B are diagrams showing an example of DNA information digitalized.

Referring to FIG. 4A, FIG. 4A illustrates an example of converting adenine (A), guanine (G), cytosine (C), and thymine (T), which are constituent bases of DNA, into digital information, respectively. The figure shown.

Adenine (A) may be converted to digital information '00', guanine (G) to digital information '01', cytosine (C) to digital information '10', and thymine (T) to digital information '11'. Of course, this transformation is only one example of the configuration, it will be natural that the constituent base of the DNA can be digitalized by a variety of methods.

Referring to FIG. 4B, FIG. 4B is a diagram showing an example of converting a specific DNA sequence into digital information according to the rule of FIG. 4A.

3 'and 5' indicate the direction of the nucleotide sequence, and the digital nucleotide sequence 'GTAGCGTCA' is converted into '011100011001111000' by digital information. In the present invention, after storing the information of the authentication target detection substance in the management server 10, if there is a request from the client device 30 later, the information is transmitted to the client device (30). At this time, the information of the authentication target detection material may be DNA sequencing information for detecting the authentication target material, digital management of the DNA sequencing information using the method of Figures 4a and 4b management server 10 Will be stored in.

5 is a diagram illustrating an example of an authentication process performed in the authentication performance system according to the present invention.

Referring to FIG. 5, first, information about an authentication target substance to be authenticated is input to the client device 30. That is, in FIG. 5, it is desired to authenticate whether or not the test target material is 'hanwoo', so the authentication target material is 'hanwoo'. Since the client device 30 includes an input device such as a computer terminal, a mobile phone terminal, etc., the 'Korean beef' is input through the input device. The client device 30 requests the management server 10 for authentication target detection substance information stored corresponding to 'Korean cattle' when the input word 'Korean cattle' corresponding to the substance to be authenticated is input.

The authentication target detection material information means that the DNA information of the material capable of detecting the authentication target material is digitalized as described above with reference to FIGS. 4A and 4B. In FIG. 5, since it is assumed that the DNA base sequence of the detection target material capable of authenticating Korean cattle is 'AGCT', the management server 10 transmits the information of '00011011', which is digital information of the 'AGCT', to the client device ( 30).

The client device 30 receives the digitally-informed authentication target detection material information, and requests the inspection apparatus 20 to generate an authentication target detection material corresponding to the received information '00011011'. The authentication target detection substance generation unit 21 included in the inspection apparatus 20 receives the information, converts '00011011' into 'AGCT', which is the original sequencing information, and has a nucleotide sequence of 'AGCT'. Generate a detection substance.

On the other hand, the DNA extraction unit 22 included in the inspection device 20 extracts the DNA base sequence from the test target material (substance to be certified as Korean beef or not). The extracted DNA base sequence and the authentication target detection material are synthesized to determine whether the test target material is Hanwoo. The determination of the synthesis result is performed by the synthesis result analyzer 23. The 'AGCT' nucleotide sequence, which is a detection target for authentication, is a complementary DNA nucleotide sequence that reacts with the DNA nucleotide sequence included in the Korean cattle. Thus, when the test target is Korean cattle, a positive reaction is derived by the synthesis. The synthesis result analysis unit 23 analyzes the result of the synthesis and transmits the result to the client device 30.

The client device 30 notifies the user of the result, i.e. whether the test subject substance contains the same DNA information as the authentication subject substance. As a method of notifying the user of the result, various methods such as output through a display screen of the client device or using a separate printing device may be used.

In the above, the configuration of the present invention with reference to the preferred embodiment of the present invention, but the present invention is not limited to the above embodiment, of course, it is within the scope of the present invention as grasped by the appended claims and drawings Various modifications, modifications, and improvements are possible in the art to those skilled in the art.

1 is a view schematically showing the overall configuration of a system for performing authentication according to the present invention.

2 is a flowchart illustrating a process of performing authentication between a test target material and an authentication target material using DNA information generated in the authentication performance system according to the present invention.

3 is a block diagram illustrating a process of performing authentication between a test target material and a test target material that are performed in a test apparatus.

4A and 4B are diagrams showing an example of DNA information digitalized.

5 is a diagram illustrating an example of an authentication process performed in the authentication performance system according to the present invention.

Claims (13)

A method for performing authentication between a substance to be tested and a substance to be authenticated by using online DNA digital information in a client device coupled to an inspection device and a management server, A first step of receiving information of the authentication target material; Requesting authentication target detection substance information stored corresponding to the authentication target substance to the management server by using the input information; A third step of receiving the authentication target detection substance information from the management server; And A fourth step of requesting the inspection apparatus to perform a test on whether the test target material and the authentication target material include the same DNA information by using the received authentication target detection material information; Including; The test apparatus performs a test according to a request of the client device, and transmits the test result to the client device, characterized in that the authentication method between the test target material and the authentication target material using the online DNA digital information. The method of claim 1, The test as to whether the test target material and the test target material performed by the test apparatus include the same DNA information, Generating an authentication target detection material using the authentication target detection material information; Synthesizing the generated DNA detection material and the DNA material extracted from the test material; And Analyzing the synthesis result A method of performing authentication between a material to be tested and a material to be certified using online DNA digital information, characterized in that consisting of. The method of claim 1, The authentication target detection material information is a method of performing authentication between the test target material and the authentication target material using on-line DNA digital information, characterized in that the digital information DNA information. The method of claim 1, The DNA information is a method of performing authentication between the test target material and the authentication target material using on-line DNA digital information, characterized in that the DNA base sequence. The method of claim 1, And the client device is one of a computer, a mobile phone, and a notebook. The method of claim 1, In the third step, The authentication target detection material information is received through a wired or wireless network characterized in that the authentication method between the test target material and the authentication target material using the online DNA digital information. A system for performing authentication between a test substance and a test substance using online DNA digital information, A management server storing authentication target detection material information corresponding to the authentication target material; An inspection device for inspecting whether the inspection target material and the authentication target material include the same DNA information; And Receive the information of the authentication target substance, request the authentication target detection substance information stored corresponding to the authentication target substance to the management server by using the input information, and receives the authentication target detection substance information from the management server And using the received authentication target detection material information, requesting the inspection device to perform a test on whether the test target material and the authentication target material include the same DNA information. Authentication performing system between the test target material and the test target material using the online DNA digital information comprising a. The method of claim 7, wherein The test as to whether the test target material and the test target material performed by the test apparatus include the same DNA information, Generating an authentication target detection material using the authentication target detection material information; Synthesizing the generated DNA detection material and the DNA material extracted from the test material; And Analyzing the synthesis result Authentication performing system between the test target material and the target material using the on-line DNA digital information, characterized in that consisting of. The method of claim 8, The inspection device, An authentication object detection material generator configured to generate the authentication object detection material; A DNA extracting unit extracting a DNA material from the test target material; And Synthesis result analysis unit for analyzing the synthesis result of the authentication object detection material generated from the authentication target detection material unit and the DNA material extracted from the DNA extraction unit Authentication performing system between the test target material and the test target material using the on-line DNA digital information comprising a. The method of claim 7, wherein The authentication target detection material information is a system for performing authentication between the test target material and the authentication target material using on-line DNA digital information, characterized in that the digital information DNA information. The method of claim 7, wherein And the DNA information is a DNA base sequence. The authentication performing system between the test target material and the test target material using the online DNA digital information. The method of claim 7, wherein And the client device is one of a computer, a mobile phone or a notebook. The method of claim 7, wherein When the client device receives the authentication target detection material information from the management server, A system for performing authentication between a substance to be tested and a substance to be authenticated using online DNA digital information, which is received through a wired or wireless network.

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