KR20100075293A - Method for preventing growth of algae using nerium genus plant extract - Google Patents

Abstract

PURPOSE: A method for effectively suppressing algae proliferation using Nerium genus plant extract is provided. CONSTITUTION: A method for suppressing algae proliferation comprises a treatment step with 150ppm-400ppm of Stephanodiscus Nerium genus plant extract or a treatment step with 400ppm-600ppm of Microcystis aeruginosa Nerium genus plant extract. The algae live in cooling water. The algae are stephanodiscus or mycrocystis aeruginosa.

Description

Method for preventing growth of algae using Nerium Genus Plant Extract}

The present invention relates to a method for inhibiting algae growth using the extract of the oleander plant, and more particularly, the environmentally friendly and economical treatment is possible by varying the concentration of the oleander plant extract for inhibiting algal growth according to the type of algae. It's about how to do that.

Hot water cooling systems are relatively slow in microbial growth because of the relatively low concentrations of nutrients essential for microbial growth, while open-circulation cooling systems introduce microbes in the air and concentrate the nutrients in the supplemental water through evaporation. Faster microbial growth. In this open-circulation cooling system, organic-inorganic nutrients, appropriate temperature and pH ranges, and continuous air supply provide an ideal environment for microbial growth, resulting in the emergence of various microbial populations. At this time, if the microorganisms are not suppressed, slime is formed and the flow of cooling water is disturbed, or the scale is secondaryly concentrated by the algae killing body.

Slime obstacles use nutrients dissolved in water to grow microorganisms such as bacteria, filamentous fungi, and algae, and microorganisms such as soil and dust attach to the microorganisms mainly to make swelling pollutants. Attaching or depositing to air or pipes causes a decrease in heat exchange efficiency, a decrease in flow rate due to an increase in pressure loss, and pitting. The slime obstacle phenomenon in the cooling system is due to the action of the microorganisms produced by the microorganisms, and the slime-attached fouling caused by the mixture of microorganisms and earth and sand is added to the solid surface. It can be classified as slime deposit fouling caused by contaminants generated.

In addition, the second-order scale disorder caused by the algae killing agent is concentrated in carbonic acid, sulfuric acid, calcium phosphate, zinc phosphate, and silica dissolved in cooling water, and the solubility decreases with increasing pH and temperature. It is a phenomenon that precipitates are formed and adhered due to the difference in saturation solubility in the heat transfer surface of the heat exchanger. Water with high hardness is mainly formed of carbonate scale on the heat exchanger's heat transfer surface when the water temperature or the concentration of cooling water is increased, and growth of the water is promoted by floating solids. This scale lowers the heat transfer efficiency, thereby increasing the condenser temperature of the refrigerator and increasing the power consumption of the refrigerator due to the decrease in COP.

In the conventional cooling water system, microbicides used as a method for preventing microorganisms include oxidizing biocides and nonoxidizing biocides.

Oxidizing biocide has a strong oxidizing power to oxidize microbial cell constituent proteins to sterilize. Chlorine (or a combination of chlorine and bromine) and monochloramine are mainly used. Chlorine and bromine are strong oxidants (acutely toxic) with short half-lives, but they come into contact with halogens to form halogenated by-products. Other oxidizing fungicides include ozone, ultraviolet light, hydrogen peroxide and peracetic acid, which require pretreatment of supplemented water or additives, which are less harmful than halogenated fungicides but are more expensive in all situations. The drawback is that it is not applicable.

In the chlorine sterilization method most commonly used as a cooling water disinfectant, the concentrations of HOCl and OCl- change with pH, and HOCl has better sterilization ability than OCl-. As the pH increases, the ratio of OCl- to HOCl increases, which reduces the sterilization effect of chlorine disinfectant. Therefore, when the pH of the cooling water is high, various measures to increase the disinfection effect are necessary. However, since chlorine is corrosive to the metal material of the cooling system, the residual chlorine should be 1 mg / L or less, and the treatment time should be as short as possible. Therefore, even if chlorine treatment is performed, it is impossible to completely prevent the growth of microorganisms, so a formulation having no problem of corrosion other than chlorine is required. In addition, in the chlorine sterilization method, the sterilization ability may be greatly reduced by reaction with ammonia or amine chemicals present in the cooling water. The chlorinated amine, the reaction product, has very poor sterilization ability.

Nonoxidizing biocide exerts bactericidal activity by inhibiting the metabolism of microorganisms. Non-oxidizing fungicides include isothiazolones, methylene bisthiocyanate, glutaraldehyde, and quaternary ammonium. It has the characteristic that it is. Most of these substances are acutely toxic and most are not readily biodegradable, although some may be hydrolyzed or degraded by other mechanisms.

Therefore, in order to solve these problems, it is necessary to develop an eco-friendly and economical treatment method by controlling algae using an agent that does not cause secondary pollution, while oleanders and plants that can be used to suppress the growth of algae are toxic. It is a very strong plant and is known to cause fatal doses even when animals consume a small amount of 0.005% of their body weight (Turner and Szczawinski, 1999). Therefore, it is desirable to suppress the growth of algae by using the extract of the oleander family in an effective concentration for the individual algae, and therefore, it is required to set the effective concentration of the extract of the oleander family to control the growth of the target algae.

Accordingly, the present invention is to provide a method for inhibiting algae growth that enables cooling water management using environmentally friendly preparations to control algae in cooling system water using fresh water.

According to the present invention, there is provided a method for inhibiting algal growth using oleander plant extract.

According to the present invention, it is possible to expect the effect of managing the cooling water in an eco-friendly and economical manner by using the extract of the oleander and plant without using an expensive microbial treatment agent or pesticide in a specific concentration.

According to the present invention, there is provided a method for inhibiting algae growth in cooling water using a oleander plant extract comprising the step of treating the oleander plant extract in cooling system water.

Hereinafter, the present invention will be described in detail.

The present invention relates to a method for inhibiting algae growth using the oleander plant extract, the extract of the oleander plant that can be used in the present invention is a cardioactive glycoside component extracted from each organ, including flowers, leaves, branches and roots of the oleander plant It can be extracted from oleander plants containing phosphorus oledroside and nerioside as active ingredients.

It is in the apocynaceae plant is limitation, for example, oleander (Nerium indicum), mancheop oleander (Nerium indicum for. Plenum), yellow oleander (Nerium indicum for. Lutescens), white oleander (Nerium indicum for. Leucanthum) and It may be selected from the group consisting of Nerium oleander .

The growth of algae in cooling water is considered to be inhibited by the action of cardioactive glycosides, in particular oledroside and nerioside, which act as a main component of oleander and plant extracts, which act to block the metabolism of life.

Oleander plant extract is a common method of extracting active ingredients from plants and is extracted from each organ of the plant of the oleander family including flowers, leaves, branches, stems, and roots. A general extraction method may be used, but is preferably extracted using hydrogen peroxide.

As a preferred extraction method, first, the anti-microbial active ingredient cardioactive glycoside component, olendroside and nelioside components are contained in each organ of the plant such as flowers, leaves, branches, stems and roots. The engine is dried to remove moisture, pulverized into powder, and distilled water is added to the pulverized plant, stirred for several days, left to stand, and filtered to obtain a filtered solution as a stock solution.

As another preferred extraction method, the extraction method in the case of using hydrogen peroxide, for example, after drying the oleander and plants, pulverized the dried oleander and plants, and adding hydrogen peroxide to the pulverized plant, stirred and left to stand for several days. Thereafter, water may be added, mixed and then filtered. After diluting the filtered solution as a stock solution at an appropriate ratio, it can be applied to the cooling water to be treated.

The oleander and plant extract obtained by the above method can be applied to any application to suppress the growth of algae, and may be preferably used to suppress the growth of bacteria, especially in a cooling system using fresh water as industrial water, It does not limit to this. The method of applying the oleander plant extract in the cooling water is not particularly limited, and may be applied by any method known in the art, such as spraying and spraying.

Algae that can inhibit the growth by applying the oleander plant extract is not limited to this, cyanobacteria, including cyclosistis aeruginosa ( Cylotella sp.) Stefanodiscus (Stephanodiscus) Diatoms , including, but not limited to, Fragilaria , Asterionella formosa , Nitzschia and Synedra sp., Including Synedra acus . Includes variants of The cooling water contains a number of algae that can be found in Table 1, and these algae are derived from raw water and differ in the type of algae included according to the inflow time of raw water.

That is, the algae present in the cooling water are determined by the algae introduced from the raw water, and the community dynamics of the algae are greatly influenced by the water temperature, and thus the microcistis aeruginosa in the summer of April to September when the temperature rises. ( mycrocystis aeruginosa ) is a thriving South African colony, and during the winter months of November through March, Cyclolotella sp. Stephanodiscus, Fragilaria , Asterionella formosa diatom community, which can be represented by Synedra sp., including formosa , Nitzschia , and Synedra acus , show predominant characteristics. These algal colonies cause impairment of water purification and are closely linked to the efficiency of water purification.

Furthermore, the concentration of the oleander and plant extract required for its inhibition is different depending on the type of algae. That is, as can be seen in Table 2, Figures 1 and 2, diatoms represented by Stephanodiscus (Stephanodiscus) showed an inhibitory effect when the concentration of oleander and plant extract is more than 150 ppm, while microcistis eruginosa Cyanobacteria represented by ( mycrocystis aeruginosa ) showed an inhibitory effect when the concentrations of oleander plant extracts were higher than 400 ppm. Therefore, the oleander plant extract has the effect of inhibiting the growth of algae, but it can be confirmed that the concentration of the oleander plant extract required for effective inhibition according to the type of algae is different.

Therefore, the oleander family extract is added to dilute to a concentration range of 150 ppm or more and preferably 150 to 400 ppm in the cooling water in the case of diatoms containing Stephanodiscus and Synedra sp., 150 ppm or less In the case of the addition, the effect of inhibiting the growth of bacteria in the cooling water is reduced, and the cost is increased when the input amount is more than 400ppm.

On the other hand, in the case of cyanobacteria containing mycrocystis aeruginosa is added to the cooling water diluted to more than 400 ppm and preferably in the concentration range of 400 to 600ppm, when added to 400 ppm or less bacteria in the cooling water The effect of inhibiting proliferation is reduced, and the cost is increased when the dose is more than 600ppm.

Hereinafter, the present invention will be described in detail through examples. The following examples illustrate the invention but do not limit the invention.

Example 1 Preparation of Oleander Plant Extract

Leaves of Nerium Indicum, an oleander plant, were collected from native plants, dried in the shade, and then powdered to 3 mm or less using a grinder. First, the leaves of the oleander plant were dried at 70 ° C. for 48 hours to remove moisture, and then pulverized into powders of 3 mm or less. The solution filtered in this manner was used as a stock solution, which was diluted to an appropriate ratio and applied to the algae to be removed.

Example 2: Characterization and Identification of Algae Isolated from Cooling Water

Water samples were collected from a water tank, which is the source of cooling water, and the species of algae that survived the water samples were inoculated by diluting raw water with sterilized water stepwise and inoculated by sterilization. Post colony producing bacteria (CFU) was measured. The characteristics and identification results of the dominant alga are shown in Table 1. As a result, algae was the most prevalent in 51% of microcistis eruginosa in summer, and stefanodiscus was 92% in winter.

In the table below, summer represents April to September and winter represents November to March.

Table 1. Characteristics and Identification of Algae Isolated from Cooling Water

number Name of bird Summer distribution ratio (%) / CFU Winter distribution ratio (%) / CFU Optimal temperature of growth ( ^oC ) One Microcystis aeruginosa 51 30 2 Stefanodiscus
(Stephanodiscus) 17 92 3 Staurastram
( Staurastrum sp. ) 16 4 Asterionella formosa 2 5 Melosira Granulata
( Melosira granulata ) 10 6 Cinedra Accus
( Synedra acus ) 0.5 7 Dinobrion Sertularia
( Dinobryon sertularia ) 1.5 8 8 Senedesmus
( Scenedesmus sp. ) 0.7 9 Cryptomonas
( Cryptomonas sp. ) One Sum 100 100

Example 3: Degree of inhibition by concentration of oleanderaceae plant extracts according to the type of algae

Stepianodiscus , the dominant species of algae identified in Example 2, and mycrocystis aeruginosa were added to the medium of the oleander family plant extract of Example 1 to the medium, as shown in Table 2 below. After the final concentration of the inoculated purely isolated bacteria were inoculated by shaking culture (30 ℃, 150rpm) and the algae population was measured every 24 hours.

Table 2. Degree of Inhibition of Algae According to the Concentrations of Oleander Plant Extracts

Concentration of extract (ppm) Stefanodiscus
( Stephanodiscus )
Suppression degree (%) Microcystis Eruginosa
degree of inhibition of ( mycrocystis aeruginosa ) 0 0 0 100 21 0 150 100 0 200 100 0 250 100 0 300 100 0 350 100 32 400 100 100 500 100 100

As can be seen in Table 2, the algal growth inhibition effect of the algae in the cooling water using the oleander plant extract was different in the range of effective concentration to suppress, depending on the type of algae, the case of Stephanodiscus algae In the case of more than 150ppm showed 100% inhibitory effect, but in the case of mycrocystis aeruginosa ( mycrocystis aeruginosa ) showed a 100% inhibitory effect when used at a concentration of 400ppm or more.

Therefore, according to the method of the present invention, algae can be effectively controlled by using different effective concentrations of oleanders and plant extracts according to predominant times of algae and algae that cause biofouling in cooling water. In addition, the oleander extract of the present invention is biodegradable, so the secondary pollution problems such as concentration of the hardly degradable substances exhibited during the disaster prevention by conventional chemical treatment and soil contamination thereby are solved. Therefore, it is possible to prevent the use of excess extract unnecessarily and economically suppress the growth of algae.

Figure 1 shows the degree of inhibition of Stephanodiscus hanchi ( Stephanodiscus hantzchii ) according to the concentration of the oleander family extract.

Figure 2 is a microcystis eruginosa ( mycrocystis) according to the concentration of oleander plant extract aeruginosa ) is the degree of inhibition.

Claims (5)

Algal growth inhibition method comprising the step of treating with oleander plant extract. The method according to claim 2, wherein the algae are algae that live in cooling water. The method of claim 2, wherein the algae is at least one selected from the group consisting of stephanodiscus and mycrocystis aeruginosa . 4. The method for inhibiting algal growth according to claim 3, wherein the stepan oleander plant extract is treated with stephanodiscus at a concentration of 150 ppm to 400 ppm. The method of claim 3, wherein the microcystis aeruginosa is treated with oleander plant extract at a concentration of 400 ppm to 600 ppm.

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