KR20100071397A - Healthy food composition containing the cactus extract for non-toxic, antioxidant active and antiobese active - Google Patents

Abstract

PURPOSE: A health food composition with non-toxic, antioxidant and antiobesity activity and a manufacturing method thereof are provided to improve antioxidant and antiobesity activity with safety by adding cactus extract in the health food. CONSTITUTION: A health food composition contains cactus extract which is Opuntia ficus-indica P.Miller, Opuntia spec. or Echinocactus grusonii. The cactus extract is manufactured by concentrating a solvent selected from refined water or alcohol of 8 ~ 12 times of a cactus volume with the cactus in 80~100°C for 6 ~ 10 hours.

Description

Health food composition containing the cactus extract for non-toxic, antioxidant active and antiobese active}

The present invention relates to a health food composition having a non-toxic, antioxidant and anti-obesity activity containing a cactus extract and a method for preparing the same, and more specifically, a cactus extracted from any one cactus selected from the group consisting of bogeum, daekyung and kumho. It relates to a health food composition and a functional beverage composition having a non-toxic, antioxidant and anti-obesity activity containing the extract and a method for producing a health food composition comprising the step of extracting the cactus extract.

Cactus is a generic name for plants belonging to the dicotyledonous cactus Cactus family, and it is a flowering plant that is characterized by fleshy large stems without leaves. Cactus (Opuntia ficus-indica var.saboten), which is common in Korea, is a tropical mountain, 2m high, with many flat branches, growing on Jeju Island. The femur is dark green, and the shape is elliptical or the egg upside down and is flat. 2 ~ 5 spines of 1-3cm long, with hairs next to them, but old ones become thick like trees and flat branches spread out in all directions. In summer, yellow flowers grow on the upper edge of the ganglia. Calyxes, petals, and stamens are many, with one pistil. The ovary is the lower part, and the fruit is berry, which looks like a pear, contains many seeds, and can be eaten, but is medicinal or mostly eaten by birds in the tropics.

In Korea, since the development of various health foods using palm cactus in the 90s, its use has been diversified, and it has become a plant that satisfies both medicinal and edible at the same time. In particular, pain, boils, early treatment of non-equipment, relieves kidney and stomach pain, detoxification of snake venom, antipyretic relief, sore throat relief, burn treatment, soothing acute symptoms, anti-inflammatory action, treatment of acute mastitis, callus removal, I) The possibility of development is even greater because it is already recognized as a folk remedy in the flow of blood, the promotion of blood circulation, the elimination of blood, the effect of the branch office, and the treatment of enteritis. In Mexico, where research on cactus is most active, many studies have been conducted, including the development of capsules and tablets as a medicinal treatment. In addition, farmers eat cactus stems or berries when they are tired or thirsty, and are recovering surprisingly fast. They are popular as energetics, and women are gaining attention as diets, skin care agents, and constipation treatments. It is known to be effective in preventing heart disease and adult disease in the United States, and active research is being conducted, such as a worldwide academic conference every two years.

In particular, diabetics who do not rely on insulin are known to be able to reduce their blood sugar by ingesting stems and reducing their sugar content by increasing insulin activity even in high sugar conditions. In addition, it can reduce the contents of low-density lipid protein, cholesterol, and triglycerides in the blood, which has great potential as a crop. In addition, palm cactus contains more ingredients such as dietary fiber than other vegetables and fruits as shown in the chart, and it is analyzed that it is effective in preventing constipation and activating bowel movement. In addition, the content of phenolic substances and flavonoids, which are closely related to human aging and cancer development, are much higher than that of ginger and rootroot, and the dietary fiber content involved in preventing constipation and intestinal activation is also high in grains, fresh vegetables, and fruits. Compared with much higher than that, vitamin C is also found to contain a lot of drinks, processed foods, pharmaceuticals, etc. can be developed that much.

A lot of studies on cactus having various effects have already been made, and the application to foods such as gems, jelly, juice, and other processed products using cactus and its application to medicinal products such as anticancer agents, hypoglycemic agents, anti-inflammatory and analgesics The situation is being actively researched. Therefore, more diverse applications using cactus and the research and development of new efficacy that have not yet been identified are needed.

An object of the present invention is to provide a cactus extract having a different effect or better than the conventionally known efficacy of cactus, and to provide a beverage and a method for producing the same using a cactus extract having such an excellent effect.

To solve the above problems,

The present invention provides a health food composition having a non-toxic, antioxidant and anti-obesity activity containing a cactus extract extracted from any one selected from the group consisting of bogeum, daekyung and kumho, and functional beverage composition comprising the cactus extract To provide.

In another aspect, the present invention provides a method for producing a health food composition having a non-toxic, antioxidant and anti-obesity activity, characterized in that it comprises the step of extracting the cactus extract from any one cactus selected from the group consisting of bogeum, Daekyung and Kumho. And it provides a method for producing a functional beverage composition comprising the step of adding a cactus extract prepared by the above method.

The composition containing the cactus extract of the present invention is not toxic and safe as well as serves as a health food having the function of antioxidant and anti-obesity, and provides a functional beverage composition with excellent taste and nutrition containing cactus extract. In addition, it provides an efficient extraction method of cactus extract, and is expected to contribute to the application to various food industries using this.

The present invention provides a non-toxic, antioxidant and anti-obesity activity containing cactus extract extracted from any one cactus selected from the group consisting of Opuntia ficus-indica P. Miller , Opuntia spec and Kumho ( Echinocactus grusonii ). Eggplant provides a health food composition.

In the present invention, the cactus extract is added to any one or more solvents selected from purified water or alcohol to 8 to 12 times the volume of the cactus and extracted at 80 ~ 100 ℃ for 6 to 10 hours, the extract was concentrated to 60 to 80 Brix Cactus concentrate.

The present invention also provides a functional beverage composition comprising the cactus extract.

The present invention provides a method for producing a health food composition having a non-toxic, antioxidant and anti-obesity activity, comprising extracting a cactus extract from any one cactus selected from the group consisting of bogeum, daegyeong and kumho. .

In the present invention, the manufacturing method comprises the steps of preparing and washing and cutting any one of the cactus selected from the group consisting of bogeum, large diameter and Kumho; and at least one solvent selected from purified water or alcohol in the prepared cactus 8 ~ Adding 12 times, extracting at 80-100 ° C. for 6-10 hours to obtain a cactus extract; And concentrating the cactus extract to 60 to 80 Brix to obtain a cactus concentrate.

In another aspect, the present invention provides a method for producing a functional beverage composition comprising the step of adding a cactus extract prepared by the above method.

Hereinafter, a health food composition having a non-toxic, antioxidant and anti-obesity activity containing the cactus extract of the present invention, a functional beverage composition using the cactus extract, and a method of preparing these health food compositions and functional beverage compositions will be described in detail.

The present invention verifies the physiological activity and stability of the cactus in order to develop a cactus grown in Goyang as a processed food and its raw materials, and through this, functional food using a cactus having high physiological activity is ensured. It is about. In addition, the present invention relates to examples and experimental examples for proving the efficacy of cactus, which is not yet known, in addition to the known known effects, and beverages using the extract of the cactus having such a useful function and a method of preparing the same. .

The present invention provides a health food composition having a non-toxic, antioxidant and anti-obesity activity containing a cactus extract extracted from any one selected from the group consisting of bogeum, Daekyung and Kumho. Types of cactus include topic, night, Dangin, duodenum, wasong, sprain, sun moon gold, black temple, waltoy, annual report, phoenix, wildness, bouillon, blue sol, baeknyeoncho, acid odor, non-wood and delta, etc. Cactus showing non-toxic, antioxidant and anti-obesity activity, which are the effects of, may be referred to as Bogeum, Daekyung and Kumho.

In the present invention, the cactus extract is added to any one or more solvents selected from purified water or alcohol to 8 to 12 times the volume of the cactus and extracted at 80 ~ 100 ℃ for 6 to 10 hours, the extract was concentrated to 60 to 80 Brix Cactus concentrate.

Preferably, the cactus extract is obtained by adding 10 times the volume of the cactus to a solvent mixed with purified water and alcohol at a ratio of 95: 5, extracting at 90 ° C. for 8 hours to obtain a cactus extract, and concentrating the extract to 70 Brix. Cactus concentrate.

The present invention also provides a functional beverage composition comprising the cactus extract. The present invention can contribute to the improvement of health of the general public who drink it by providing a beverage composition having the antioxidant and anti-obesity activity and non-toxic efficacy of Bogeum, Daegyeong, and Kumho.

The functional beverage composition containing the cactus extract may further include liquid fructose, honey, royal jelly extract, citric acid, calcium lactate, vitamin C, flavoring (stevitene fresh), xylitol, and purified water. In addition to these components it may further comprise a general additive added to the functional beverage.

Preferably, the functional beverage composition is 1 to 10% by weight of cactus extract, 10 to 20% by weight of liquid fructose, honey 0.5 to 1.0% by weight, royal jelly extract 0.5 to 1.0% by weight, citric acid 0.05 ~ 0.15% by weight, calcium lactate 0.05 ~ 0.15% by weight, vitamin C 0.01 ~ 0.05% by weight, fragrance (stebiten fresh) 0.01 ~ 0.03% by weight, xylitol 0.1 ~ 0.5% by weight and the balance can be included.

More preferably, the functional beverage composition is based on the total weight of the beverage composition, cactus extract 5% by weight, liquid fructose 15% by weight, honey 0.7% by weight, royal jelly extract 0.7% by weight, citric acid 0.1% by weight, calcium lactate 0.1% by weight It may include 0.03% by weight of vitamin C, 0.02% by weight of fragrance (stevitene fresh), 0.3% by weight of xylitol and the balance of purified water.

The present invention provides a method for producing a health food composition having a non-toxic, antioxidant and anti-obesity activity, comprising extracting a cactus extract from any one cactus selected from the group consisting of bogeum, daegyeong and kumho. .

In the present invention, the preparation method comprises the steps of preparing and washing and cutting any one cactus selected from the group consisting of bogeum, large diameter and Kumho; and at least one solvent selected from purified water or alcohol to the prepared cactus Adding to 12-fold, extracting at 80-100 ° C. for 6-10 hours to obtain a cactus extract; And concentrating the cactus extract to 60 to 80 Brix to obtain a cactus concentrate.

Preferably, the preparation method comprises the steps of preparing and washing and cutting any one cactus selected from the group consisting of bogeum, Daekyung and Kumho; and a solvent in which purified water and alcohol are mixed in a ratio of 95: 5 to the prepared cactus. Adding 10 times the cactus volume and extracting at 90 ° C. for 8 hours to obtain a cactus extract; And concentrating the cactus extract to 70 Brix to obtain a cactus concentrate.

This is because the extraction and concentration conditions increase the extraction yield of the cactus extract, so that it can be preserved for more than 12 months at room temperature. In addition to the solvent extraction method, it is possible to produce a cactus extract using a known extraction method.

The present invention also provides a method for producing a functional beverage composition comprising the step of adding a cactus extract prepared by the above method.

Method for producing the functional beverage composition in the present invention comprises the steps of extracting the cactus extract from any one cactus selected from the group consisting of bogeum, Daegyeong and Kumho; and liquid fructose, honey, royal jelly extract, citric acid, lactic acid in the cactus extract It may include; adding calcium, vitamin C, flavoring (stevitene fresh), xylitol and purified water.

Preferably, the method for preparing the functional beverage composition is based on the total weight of the beverage composition, cactus extract 1-10% by weight, liquid fructose 10-20% by weight, honey 0.5-1.0% by weight, royal jelly extract 0.5-1.0% by weight, citric acid 0.05% to 0.15% by weight, 0.05% to 0.15% by weight of calcium lactate, 0.01% to 0.05% by weight of vitamin C, 0.01% to 0.03% by weight of fragrance (stevitene fresh), 0.1% to 0.5% by weight of xylitol, and the remaining purified water can be added.

More preferably, the method for preparing the functional beverage composition is based on the total weight of the beverage composition, cactus extract 5% by weight, liquid fructose 15% by weight, honey 0.7% by weight, royal jelly extract 0.7% by weight, citric acid 0.1% by weight, calcium lactate 0.1 weight%, 0.03 weight% of vitamin C, 0.02 weight% of fragrance | flavor (steatten fresh), 0.3 weight% of xylitol, and remainder purified water can be added.

Hereinafter, the Examples and Experimental Examples of the present invention. The following Examples and Experimental Examples are intended to explain the present invention in more detail, and the scope of the present invention is not intended to be limited thereto.

Example 1 Preparation of a Composition Having Non-Toxic, Antioxidant and Anti-obesity Activity Containing Bochum Extract

1. The cactus bogeum is cultivated in Goyang-si and used for the practice of the present invention.

2. Wash the 1kg of the boil with water and cut the washed bogeum.

3. The solvent was extracted at 90 ° C. for 8 hours by adding a mixed solvent of purified water 19 L and ethanol 1 L to the cut test. The supernatant was centrifuged to obtain a bogeum extract.

4. The bogeum extract was further heated to concentrate the bogeum extract to 70 Brix.

Example 2 Preparation of a Composition Having Non-Toxic, Antioxidant and Anti-obesity Activity Containing Large Diameter Extract

1. A cactus bayonet is cultivated in Goyang-si and used for the practice of the present invention.

2. Wash the large diameter 1 kg with water and cut the washed large diameter.

3. A mixed solvent of purified water 19 L and ethanol 1 L was added to the cut large diameter, and the solvent was extracted at 90 ° C. for 8 hours. The supernatant was centrifuged to obtain a large diameter extract.

4. The large diameter extract was further heated to concentrate the large diameter extract to 70 Brix.

Example 3 Preparation of a Composition Having Non-Toxic, Antioxidant and Anti-obesity Activity Containing Kumho Extract

1. Kumho, which is a cactus, is cultivated in Goyang-si and used for the practice of the present invention.

2. Wash 1 kg of Kumho well with water and cut the washed Kumho.

3. The solvent was extracted at 90 ° C. for 8 hours by adding a mixed solvent of purified water 19 L and ethanol 1 L to the cut Kumho. The supernatant was centrifuged to obtain a Kumho extract.

4. The Kumho extract was further heated to concentrate the Kumho extract to 70 Brix.

Example 4-11 Preparation of Functional Beverage Composition Added Bogeum Extract

1. A beverage composition was prepared using the bogeum extract obtained in Example 1. Additives to be added were also purchased from commercial marts or additive stores with known commercially available materials.

2. In Example 4, a bogeum extract was obtained from a bogeum, and 15 mL of liquid fructose, 0.7 mL of honey, 0.7 mL of royal jelly extract, 0.1 mL of citric acid, 0.1 mg of calcium lactate, and 0.03 mg of vitamin C were added to 5 mL of the bogeum extract. (Stebiten fresh) 0.02 mg, 0.3 ml of xylitol and 78.05 ml of purified water were added to prepare a beverage.

3. A beverage was prepared in the same manner as in Example 4, except that only the content of bogeum extract and the content of purified water were prepared (Example 5-8) (see Table 1 below).

4. Also, a beverage was prepared in the same manner as in Example 4, except that only the content of liquid fructose and the content of purified water were prepared (Example 9-11) (see Table 2 below).

[Table 1] Preparation of beverages with different content of bogeum extract (unit: ml)

Bogum Extract Purified water Remarks Example 5 One 82.05 The remaining ingredient content is the same as in Example 4
Example 6 3 80.05 Example 7 7 76.05 Example 8 9 74.05

[Table 2] Production of beverages with different content of liquid fructose (unit: ml)

Liquid fructose Purified water Remarks Example 9 5 88.05 The remaining ingredient content is the same as in Example 4 Example 10 10 83.05 Example 11 20 73.05

<Example 12-19> Preparation of the functional beverage composition to which the large diameter extract is added

1. Using the large diameter extract obtained in Example 2 to prepare a beverage composition. Additives to be added were also purchased from commercial marts or additive stores with known commercially available materials.

2. In Example 12, a large diameter extract was obtained from a large diameter, and 5 ml of the large diameter extract was 15 ml of liquid fructose, 0.7 ml of honey, 0.7 ml of royal jelly extract, 0.1 mg of citric acid, 0.1 mg of calcium lactate, and 0.03 mg of vitamin C. (Stebiten fresh) 0.02 mg, 0.3 ml of xylitol and 78.05 ml of purified water were added to prepare a beverage.

3. A beverage was prepared in the same manner as in Example 12, except that only the content of the large diameter extract and the content of purified water were prepared (Example 13-16) (see Table 3 below).

4. In addition, a beverage was prepared in the same manner as in Example 12, except that only the content of liquid fructose and the content of purified water were prepared (Examples 17-19) (see Table 4 below).

[Table 3] Production of beverages with different contents of large diameter extract (unit: ml)

Large Diameter Extract Purified water Remarks Example 13 One 82.05 The remaining ingredient content is the same as in Example 12
Example 14 3 80.05 Example 15 7 76.05 Example 16 9 74.05

[Table 4] Production of beverages with different content of liquid fructose (unit: ml)

Liquid fructose Purified water Remarks Example 17 5 88.05 The remaining ingredient content is the same as in Example 12 Example 18 10 83.05 Example 19 20 73.05

Example 20-27 Preparation of a Functional Beverage Composition Added Kumho Extract

1. A beverage composition was prepared using the Kumho extract obtained in Example 3. Additives to be added were also purchased from commercial marts or additive stores with known commercially available materials.

2. In Example 20, a Kumho extract was obtained from Kumho, and 5 ml of the Kumho extract was added 15 ml of liquid fructose, 0.7 ml of honey, 0.7 ml of royal jelly extract, 0.1 mg of citric acid, 0.1 mg of calcium lactate, and 0.03 mg of vitamin C. (Stebiten fresh) 0.02 mg, 0.3 ml of xylitol and 78.05 ml of purified water were added to prepare a beverage.

3. A beverage was prepared in the same manner as in Example 20, except that only the content of Kumho extract and the content of purified water were prepared (Examples 21-24) (see Table 5 below).

4. Also, a beverage was prepared in the same manner as in Example 20, except that only the content of liquid fructose and the content of purified water were prepared (Examples 25-27) (see Table 6 below).

[Table 5] Production of beverages with different content of Kumho extract (unit: ml)

Kumho Extract Purified water Remarks Example 21 One 82.05 The remaining ingredient content is the same as in Example 20
Example 22 3 80.05 Example 23 7 76.05 Example 24 9 74.05

[Table 6] Production of beverages with different content of liquid fructose (unit: ml)

Liquid fructose Purified water Remarks Example 25 5 88.05 The remaining ingredient content is the same as in Example 20 Example 26 10 83.05 Example 27 20 73.05

Experimental Example 1

1) Toxicity Confirmation Test by Apoptosis, 2) Mutagenicity and Antimutagenicity Test, 3) Total Polyphenol Content Measurement Test, 4) Electronic A donation (DPPH) measurement experiment, 5) pancreatic lipase activity inhibitors and inhibitory activity measurement, 6) skin regeneration and anti-inflammatory activity test and 7) anti-diabetic and thrombolytic activity test.

1. Toxicity test by cell death

(1) Obtained extracts (samples) prepared in Examples 1-3, each extract was re-dissolved in DMSO in units of 100ug / ml, and 10ul of the solution was used to proliferate in a 30mm plate of osteoblastoma U2OS Was treated. At this time, the culture medium was treated with 1% penicillin / streptomycin (Invitrogen, UK) in Dulbecco's modified Eagle's Medium containing 10% fetal bovine serum (Hyclone, Logan, UT) and etoposide 20 as a control. Only uM and DMSO were treated. Twenty four hours after each sample was treated, the cells were observed for proliferation by using an optical microscope, and the toxicity of each sample was determined according to the condition of the cells. [No effect (-)] and mild toxicity (+) ], Toxic [++], and very toxic [+ (++)]. The results of cytotoxicity by the apoptosis were shown in Table 7 below.

TABLE 7 Cytotoxicity Results 1

sample Treatment concentration (ug / ul) Cytotoxicity
Bog

4.0 - 2.0 - 1.0 - 0.6 -
Daekyung

4.0 - 2.0 - 1.0 - 0.8 -
Kumho

4.0 - 2.0 - 1.0 - 0.8 -

(2) In addition, the cytotoxicity test of cactus, which is likely to be used only as an anticancer agent due to its high cytotoxicity among gourmet cactus, was also compared (see Table 8 below).

TABLE 8 Cytotoxicity Results 2

sample Treatment concentration (ug / ul) Cytotoxicity issue 1.0 +++ Sprain 1.0 +++ Monthly payment 1.0 +++ Black Wizard 1.0 +++ Phoenix 1.0 +++ bouillon 1.0 +++ Cheongsol 1.0 +++

(3) As can be seen in Tables 7 and 8, the stability test results using the cell line showed that all of the test, Daekyung, and Kumho were safe. Comparative topics, sprains, ilwolum, black sorcerers, phoenix, bouillon, blue sol, etc. showed strong cytotoxicity even at 1ug / uL, which was impossible for food use, but showed potential for future development as anticancer agent.

2. Mutagenicity and Antimutagenicity

(1) microbial return mutation experiment

1) Each sample (cactus extract: Examples 1-3 and comparison with natural return mutations) using Salmonella typhimurium TA98 and Salmonella typhimurium TA100 according to the method of Maron and Ames (1983) The subjects were determined to have mutagenicity when the number of mutations increased by more than two times by measuring the natural number of mutations after treatment with sugar, topical extracts). For antimutagenicity, acetamidofluorene, benzo (a) pyrene, and nitrofluorene were used as mutagens. In order to determine the antimutagenicity of each cactus extract, 5.0 µl of sample was added to a sterile microtube and 500 µl of S9 mix (indirect mutagen). 500 μl of phosphate buffer (direct mutant), 100 μl of strain, and 100 μl of mutant were added sequentially and incubated for 30 minutes in a 37 ° C. water bath. This was added to 2 ml of top agar at 46 ° C. and then overlayed onto minimal glucose agar plates, and cultured in a 37 ° C. incubator for 48 hours, and the number of revertant colonies on the plate was counted.

2) The results of mutation experiments using Salmonella typhimurium TA98 and Salmonella typhimurium TA100 were as shown in FIG. 1. In the Salmonella typhimurium TA100, the control group without cactus showed 417 return mutations, and the sample was treated with Kumho 366, sugar 480, topic 500, large diameter 487, and 394 gums, which was twice the number of return mutations in the control group. It did not increase abnormally, indicating that there is no risk of mutation. In the case of Salmonella typhimurium TA98, the number of return mutations in the control group was 111, and the risk of causing mutations was not increased by more than twice the number of return mutations in the control group when the sample was processed, Kumho 95, Glucose 93, Topical 89, Large diameter 94, and Bogeum 89. Appeared to be missing. Therefore, it was found that the cactus used in this experiment was not at risk of mutation (see FIG. 1).

(2) anti mutagenicity test

1) According to the method of Maron and Ames (1983), after using the mutagen using Salmonella typhimurium TA98 and Salmonella typhimurium TA100, the results of confirming the anti-mutant activity of each cactus sample are shown in Table 9 below. And 10.

Table 9 Antimutagenic Activity of Cactus Extracts against Salmonella typhimurium TA100

Mutagens Revertants / plate Kumho Per capita issue Daekyung Bog Control 269 acetamidofluorene 365 345 356 274 356 353 Benzo (a) pyrene 313 326 425 360 377 253 Nitrofluorene 352 332 336 316 325 323

Table 10 Antimutagenic Activities of Cactus Extracts against Salmonella typhimurium TA98

Mutagens Revertants / plate Kumho Per capita issue Daekyung Bog Control 268 acetamidofluorene 324 308 271 266 279 317 Benzo (a) pyrene 341 335 334 335 348 309 Nitrofluorene 365 320 335 322 325 323

2) As can be seen from Table 9, when acetamidofluorene, benzo (a) pyrene, and nitrofluorene were treated as a mutagen in Salmonella typhimurium TA100, the number of return mutations was increased from 269 as a control to 365, 313, and 352. In the case of acetamidofluorene, the topicality showed antimutagenic activity, and in the case of benzopyrene, bogeum extract showed antimutagenic activity and no antimutagenic activity for nitrofluorene.

As can be seen in Table 10, in the case of Salmonella typhimurium TA98, when the acetamidofluorene, benzo (a) pyrene, and nitrofluorene were treated as mutagens, the number of return mutations increased from 268 to 324, 341, and 365, and the cactus extract was treated. In the case of acetamidofluorene, sugar, topical and large diameter showed anti-mutant activity, and in the case of benzopyrene, boge extract showed slight anti-mutant activity and little anti-mutant activity for nitrofluorene.

3. Total Polyphenol Content Measurement Experiment

(1) The polyphenol content, which is represented by antioxidants, was measured by modifying the Folin-densis method (Slinkard et al., 1974). That is, 0.5 ml of each sample (Example 1-3 and a topical extract) (1 mg / ml) was added to a tube, and 6.5 ml of distilled water was added. Then, 0.5 ml of folin-ciocalteu's phenol solution was added and reacted at 25 ° C. for 3 minutes. I was. In addition, 1 ml of saturated sodium bicarbonate solution and 1.5 ml of distilled water were added sequentially, and then reacted at 25 ° C. for 1 hour, and the absorbance was measured at 750 nm. The standard curve for quantifying total polyphenolic compounds was prepared using galic acid.

(2) As a result of measuring the total polyphenol content, it was measured as Kumho 7.75mg%, large diameter 1.16mg%, bogeum 5.20mg%, topic 15.56mg%.

4. Electron Donation Measurement Experiment

(1) The electron donating abilities that stop the oxidation process by removing free radicals by providing electrons as a method of measuring antioxidant power are modified by the method of Mensor et al. (2001). The reducing power of each sample was measured by the electron donating effect of DPPH (α, α-diphenyl-picrylhydrazyl) on extracts (Examples 1-3 and vitamin C, BHT compared to the topic). That is, 2.5 ml of each sample was mixed with 1 ml of 0.3 mM DPPH solution, the reaction solution was mixed for about 10 seconds, and reacted at room temperature for 10 minutes, and then absorbance was measured at 525 nm using a spectrophotometer.

(2) DPPH was measured at the concentration of 1 mg / ml for Kumho, Daekyung, Bogum, and Topicals as shown in FIG. 2, and vitamin C having antioxidant activity and BHT as a synthetic antioxidant were used as a control. That is, compared with Vitamin C and BHT, it showed about 70-80% of electron donating activity in topic, and about 30-40% in bogeum and large diameter. Therefore, it could be said that it was proved as a functional extract having some degree of antioxidant effect even if not vitamin C or BHT (see Fig. 2).

5. Screening and Inhibition of Pancreatic Lipase Activity Inhibitors

(1) Inhibiting the activity of pancreatic lipase (Pancreatic lipase) to prevent the fat absorption of the ingested fat in the body because it can be prevented from obesity due to excessive intake of fat.

Each sample prepared by Example 1-3 and the comparative cactus was prepared to have a concentration of 1 mg / ml, and then mixed with a pancreatic lipase solution and reacted for 3 minutes, and the plate medium containing tributyllin (tributylin). Four hours after dispensing on the paper disk of the clear zone (clear zone) by measuring the size of the clear ring (small zone) samples were first searched for cactus samples that inhibit the activity of pancreatic lipase was selected. The selected sample was used to measure pancreatic lipase activity inhibition activity, and the amount of oleic acid separated from triolein was measured according to the method of Bitou et al.

In other words, the substrate was prepared by adding 90 μmol triolein, 45 mg gum arabic and 9.45 μmol taurocholic acid to 9 ml of 0.1 M N-tris (hydroxymethyl) methyl-2-aminoethanesulfonic acid (pH 7.0) added with 0.1 M NaCl for 5 minutes by ultrasonic wave. Prepared by treatment. The reaction solution for measuring lipase activity was prepared by mixing 15 μl of enzyme solution (pancreatic lipase (1500 U / ml), 5 μl of plant extract, 180 μl of substrate solution) so that the volume of the final reaction solution was 200 μl, and then pH 7.0, reaction. It reacted at the temperature of 37 degreeC for 30 minutes.

Determination of oleic acid produced after enzymatic reaction was carried out by chloro-form / heptane containing 2% (v / v) methanol in 0.2 ml of the reaction solution after the enzymatic reaction according to the method of Zapf et al. : 1) 3 ml of solution was added and stirred for 10 minutes, followed by centrifugation (2,000 × g) for 10 minutes to remove the aqueous layer. 1 ml of copper reaction solution was added thereto and stirred for another 10 minutes, followed by centrifugation (2,000 × g) for 10 minutes, and 1 ml of an organic solvent layer containing oleic acid and copper salt extracted. 1 ml of 0.1% (w / v) batho-cuprione solution containing 0.05% (w / v) 3-tert-butyl-4-hydroxyanisole was added, and the absorbance was measured at 480 nm to quantify oleic acid. Enzyme activity was measured.

(2) Triglycerides, which are essentially triglycerides, must be broken down into fatty acids and glycerol in order for any ingested fat to be absorbed into the body through the intestinal wall. The action of pancreatic lipases secreted by the pancreas breaks down tri-glycerides into fatty acids and glycerol, so that the fats taken from food are absorbed into the body. Therefore, inhibiting the activity of pancreatic lipase is known to prevent obesity due to excessive intake of fat because it inhibits the body's absorption of ingested fat into the body.

In this experiment, the activity inhibitors of pancreatic lipase were searched for 22 kinds of edible and gourmet cactus grown in Goyang City, and the results are shown in Table 11 below. In other words, edible cactus showed enzymatic inhibitory activity in bogeum, Daegyeong and Kumho, and showed strong inhibitory activity of lipolytic enzymes in sugar and bouillon for food use. Activity was shown (see Table 11 below).

Table 11 Results of Pancreatic Lipase Inhibitory Activity

sample Inhibition sample Inhibition Opuntia ficus-indica P. Miller (Bomb) + Kalanchoe tomentosa
(Woltoys) + Echinocactus grusonii (Kumho) + Echeveria (salary) ++ Opuntia spec + Kalanchoe daigremontiana
(Phoenix) + Crassula capitella (topic) - Aloe mitriformis ( infertile ) + Echeveria nerenbergii ( night and night ) ++ Echeveria harmsii ( lotus ) +++ Kalanchoe thyrsifolra (per person) +++ Chamaecereus silvestrii
(Cheongsol) + Haworthia fasciata
(Duodenum) + Opuntia compressa (centennial) - Orostachys japonicus (wasong) - Chamaecereus ( mountain , yellow) + Crassula ovata (sprain) + Gymnocalycium mihanovichii (undead, enemies) + Aeonium decorum ++ Gymnocalycium mihanovichii (unbened, black) + Aeonium arboreum (Black Mage) + Hyloceruns trigonus
(delta) ++

(In Table 11, "+++ represents very strong inhibition, ++ strong inhibition, + inhibition,-no inhibition.")

(3) In addition, the inhibitory ability of the first selected pancreatin lipase activity inhibiting cactus was measured by oleic acid separated from triolein according to the method of Bitou et al., The results were as shown in FIG. Orlistat 3 was used in the experiment as a control as an anti-obesity drug.) Among the cactus, pancreatin lipase activity inhibitory effect was observed in bogeum and large diameter (see Fig. 3).

(4) In addition, in order to confirm the pancreatic lipase inhibitory ability of cheonnyeoncho and baeknyeoncho, which are currently on the market, using triolein (triolein) in the present study compared with the primary screening test results, etc. Was the same (see FIG. 4).

The natural vinegar and baeknyeoncho, which are currently in the market, have been shown to have no pancreatic lipase inhibitory activity. Rather, it has been found that there is an ingredient that promotes fat breakdown to promote fat absorption. Therefore, as shown in the results of this study, it would be possible to develop obesity-preventing foods by suppressing lipolysis using a test.

6. Skin regeneration and anti-inflammatory activity experiment

(1) In this experiment, after the cactus (bogeum, Daegyeong, Kumho, topical) extracts were treated to the cells, western blot was used to confirm anti-inflammatory action by inhibition of COX-2 and skin regeneration by COX-2 expression.

COX-2 is produced in vascular endothelial cells during infection or injury and breaks down the cell's lipid, arachidonic acid, into a substance (prostaglandin) that causes fever, pain, and other uncomfortable inflammation. Cyclooxygenase (COX) is an enzyme that synthesizes prostaglandins (PG) from arachidonic acid, and isoforms include COX-1 and COX-2. COX-1 is constantly expressed, and COX-2 is an inducible form, and COX-2 is increased in inflammation, colon, rectal cancer, and other epithelial cell cancers.

COX-2 plays an important role in the development and progression of various degenerative diseases associated with the growth and differentiation of cells, including cancer, and overexpression increases angiogenesis and metastasis in cancer tissues and prevents apoptosis. Inhibition of angiogenesis and tumorigenesis by specific inhibitors has led to cancer prevention strategies by selective regulation of this gene.

COX-2 expression is also well understood in the reports that COX-2 expression is regulated by nuclear factor kappa B, which binds to promoter regions of genes related to immune system diseases, cardiovascular disease, diabetes, and induces their gene expression as well as cancer. Can be. Therefore, the recent research direction of COX-2 has attracted much attention on the upstream signaling pathways associated with these transcriptional activation elements and places much emphasis on the search for substances that can regulate their activity.

In other respects, however, increased levels of cox-2, in relation to cell growth and differentiation, promote cell survival and cell growth. For example, an ulcer is a hole in the inner wall of the entire digestive system, including the esophagus, stomach duodenum, small intestine, large intestine, and rectum, which makes up the digestive system, and varies depending on where it occurs. You lose. Painkillers such as asprine, mortrin, and aleve, which are nonsteroidal anti-inflammatory drugs, inhibit the production of cycolooxygenease for the production of prostaglandins, a material that protects the inner wall from chemical or external damage. As the production of these substances decreases, acid can erode the stomach walls and cause ulcers. In other words, cox-2 is important for gastric ulcer repair and epithelial cell proliferation.

(2) In this experiment, it was shown that the treatment of the extract of bogeum can help the recovery of epithelial cells due to trauma by promoting the secretion of COX-2 as shown in FIG. Therefore, the existing cactus promotes cell growth by a different action from the anti-inflammatory action (inhibition of COX-2), it can be seen that the use as a therapeutic agent for ulcers such as gastric ulcers (see Fig. 5).

7. Antidiabetic and Thrombolytic Activity Test

(1) Anti-diabetic activity was determined to inhibit the production of glucose by inhibiting glucose (glucose) production in the intestine and measured the ability to lower α-glucosidase activity. And Thrombolytic activity was measured by fibrinogen (Fibrinogen) (Sigma 4883), Thrombin (Green Cross, Lyophilized Powder, 5,000 IU), and agarose using fibrin plate according to Haverkate and Trass method (1974). After the preparation, the test was performed as follows. That is, fibrin plates were prepared by adding 50 μl of thrombin to a fibrinogen-agarose solution prepared by mixing 5 ml of a 0.2% fibrinogen solution (prepared with a borate saline buffer of pH 7.5). Four rounds of pasteur pipettes with a diameter of 5 mm were made on the plate, and 20 μl of each sample was dipped at each concentration and reacted at 37 ° C. for 18 hours.

(2) Edible gums, Daekyeong, and Kumho extracts showed no antidiabetic activity due to α-glucosidase deactivation activity and no thrombolytic activity.

Experimental Example 2 Sensory Test of Functional Drink Composition Containing Cactus Extract

(1) In the following experiment using the beverage composition prepared using the above Example 4-27, the palatability test for setting the optimum blending ratio of the beverage composition containing the cactus extract was examined.

The following palatability test (Sensory test) was performed by selecting 20 experienced subjects of sensory test. Based on the 9-point scale method, these 20 palatability scores were averaged and rounded to the nearest decimal place. 12).

[Table 12] Sensory Test Results

Beverage with Bogum Extract Comprehensive Taste Drink with large diameter extract Comprehensive Taste Kumho Extract
Drink included Comprehensive Taste Example 4 8 Example 12 7 Example 20 8 Example 5 6 Example 13 5 Example 21 5 Example 6 7 Example 14 6 Example 22 6 Example 7 7 Example 15 7 Example 23 7 Example 8 7 Example 16 6 Example 24 6 Example 9 6 Example 17 5 Example 25 6 Example 10 6 Example 18 5 Example 26 6 Example 11 7 Example 19 6 Example 27 7

(2) As can be seen in Table 12, in the case of bogeum extract, the beverage composition prepared by the method of Example 4 had the highest preference. Therefore, the optimal content of bogeum extract and liquid fructose was found. In addition, the beverage composition prepared by the method of Example 12 and Example 15 in the case of large diameter extract had the highest preference. Therefore, the optimum content of large diameter extract and liquid fructose was found. In addition, the case of Kumho extract was found that the beverage composition prepared by the method of Example 20 had the highest preference.

The present invention is expected to reveal the efficacy of the cactus having a useful function and to provide a method for producing a cactus extract and a functional beverage using the same, to contribute to profit creation and export promotion to a new food industry. In addition, it is expected to contribute to national health by inspiring cactus breeders and encouraging consumers to eat a variety of foods including nutritious and tasty cactus extracts.

1 shows the number of return mutations of the cactus extract for Salmonella typhimurium TA98 and Salmonella typhimurium TA100.

2 shows the electron donating activity of the cactus extract.

Figure 3 shows the inhibitory ability of pancreatic lipase of the cactus extract using Triolein (Triolein).

Figure 4 shows the inhibitory ability of pancreatic lipase of cheonnyeoncho (root, stem, fruit) and baeknyeoncho (stem) and bogeum, sugar extract.

Figure 5 shows the expression level of COX-2 according to the treatment of the cactus extract.

Claims (6)

Health food composition having a non-toxic, antioxidant and anti-obesity activity containing cactus extract extracted from any one selected from the group consisting of bogeum, Daegyeong and Kumho. According to claim 1, The cactus extract is one or more solvents selected from purified water or alcohol is added to 8 to 12 times the volume of the cactus and extracted at 80 ~ 100 ℃ for 6-10 hours, the extract is concentrated to 60 ~ 80 Brix Health food composition having a non-toxic, antioxidant and anti-obesity activity, characterized in that the concentrated cactus concentrate. Functional drink composition, characterized in that it comprises a cactus extract extracted from any one selected from the group consisting of Bogum, Daekyung and Kumho. Method of producing a health food composition having a non-toxic, antioxidant and anti-obesity activity comprising the step of extracting the cactus extract from any one cactus selected from the group consisting of bogeum, Daekyung and Kumho. The method of claim 4, wherein Preparing by washing and cutting any one cactus selected from the group consisting of bogeum, daegyeong and kumho; Adding at least one solvent selected from purified water or alcohol to the prepared cactus at 8-12 times the volume of the cactus, extracting at 80-100 ° C. for 6-10 hours to obtain a cactus extract; And Concentrating the obtained cactus extract to 60 ~ 80 Brix to obtain a cactus concentrate; method of producing a health food composition having a non-toxic, antioxidant and anti-obesity activity comprising a. Method of producing a functional beverage composition comprising the step of extracting the cactus extract from any one cactus selected from the group consisting of bogeum, Daekyung and Kumho.

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