KR20100060949A - Composition for protecting nerve cells - Google Patents

Abstract

PURPOSE: A composition containing peach leaf extract as an active ingredient is provided to effectively prevent or treat diseases related to neural cell protection. CONSTITUTION: A composition for protecting neural cells contains peach leaf extract as an active ingredient. The peach leaf extract is isolated using water, organic solvent, or mixture solvent thereof. The peach leaf extract is methanol extract. A health food contains the composition for protecting neural cells. A method for purifying a fraction of peach leaf extract comprises: a step of extracting peach leaf; a step of sequencially fractioning with hexane, chloform, and ethylacetate; a step of decompressing and drying the fraction; a step of performing silica gel column chromatography; a step of adding chloroform and methanol to obtain peach leaf extract fraction; and a step of performing TLC(thin layer chromatography) and HPLC(high performance liquid chromatography) to obtain the peach leaf extract fraction.

Description

Composition for protecting nerve cells

The present invention relates to a neuronal cell protective composition comprising a peach leaf extract as an active ingredient.

Stroke is the third leading cause of death following heart disease and cancer and causes chronic disorders (Wong KS, Chen C, Ng PW, Tsoi TH, Li HL, Fong WC, et al.:Lowmolecular weight heparin compared with aspirin for the treatment of acute ischaemic stroke in Asian patients with large artery occlusive disease: a randomised study., Lancet Neurol (2007) 6: 407-13), although one-sixth of humans suffer from vascular disease, effective treatment for cerebrovascular disease (Seshadri S, Beiser A, Kelly-Hayes M, Kase CS, Au R, Kannel WB, et al .: The lifetime risk of stroke: estimates from the Framingham study, Stroke (2006) 37: 345-50). Ischemic cerebrovascular disease is a disorder of the blood supply that damages tissues by reducing oxygen and glucose. However, more severe tissue damage occurs when blood is re-reflowed, and the pathological mechanism of brain cell damage caused by ischemic cerebral infarction is complex and multifactorial (Maruyama et al .: Protective Properties of Neoechinulin A against SIN-1-Induced). Neuronal Cell Death, J. Biochem. 136, 81-87 (2004), Meenakshisundaram, Thiyagarajan, Chaman Lal, Kaul and Shyam Sundar, Sharma. Neuroprotective efficacy and therapeutic time window of peroxynitrite decomposition catalysts in focal cerebral ischemia in rats .: British Journal of Pharmacology, (2004) 142, 899-911). Many studies to date report that reactive oxygen species are produced in the brain during ischemia and revascularization, and active oxygen species are involved in neuronal damage after cerebral infarction (Meenakshisundaram Thiyagarajan, Shyam S. Sharma). * ,: Neuroprotective effect of curcumin in middle cerebral artery occlusion induced focal cerebral ischemia in rats, Life Sciences 74 (2004) 969-85). Some species of reactive oxygen species (ROS) are known to have a role in brain injury after cerebral ischemia, which is produced after rebleeding after humility (Imaizumi, S., Kayama, T., Suzuki, J., Chemiluminescence in hypoxic brain the first report, Correlation between energy metabolism and free radical reaction.Stroke. (1984); 15 (6), 1061-1065). In particular, neurons are susceptible to damage by ischemic injury, loss of mitochondrial function, activation of glutamate receptors, calcium overaccumulation (Cho DW, Ischemia-induced neuronal apoptosis, Curr Opin Neurobiol. (1996); 6: 667-72.).

ATP metabolism induces hypoxanthine accumulation, while xanthine oxidase is present as nicotinamide adenine dinucleotide reductive hydrogenase in tissues in which ischemia does not occur. (Morimoto, K., Tagawa, K., Hayakawa, T., Watanabe, F. and Mogami,: H. Cellular level of purine compounds in ischemic gerbil brain by high performance liquid chromatography. J. Neurochem. (1982); 38 , 833-835.). Proteolytic enzymes activated by calcium ions in ischemia irreversibly convert xanthine dehydrogenase to xanthine oxidase, and xanthine oxidant further oxidizes xanthine to generate uric acid and reactive oxygen species (Parks, DA and Granger, DN). : Xanthine oxidase:.... . biochemistry, distribution and physiology Acta Physiol Scand Suppl, (1986); 548, 87-99). NO (nitric oxide) is enzymatically synthesized from L-arginine and significantly increased by ischemia (Wei, G., Dawson, VL and Zweier, JL: Role of neuronaland endothelial nitric oxide synthase in nitric oxide generation in the brain following cerebral ischemia, Biochim. Biophys. Acta (1999); 1455, 23-34.). In addition, superoxide anion and hydroxyl radical induce lipid peroxidation to induce cell membrane destruction and iNOS (inducible nitric oxide synthase) is upregulated by re-blood flow after ischemia. Excess nitric oxide reacts with superoxide to produce peroxynitrite, and the resulting peroxynitrite freely passes through the phospholipid bilayer to react with various molecules such as lipids, proteins, and DNA. Induces death (Li, J .; Su, J .; Li, W .; Liu, W .; Altura, BT; Altura, BM: Peroxynitrite induces apoptosis in canine cerebral vascular muscle cells: possible relation to neurodegenerative diseases and strokes., Neurosci. Lett. 2003, 350 , 173-177.). This series of reactions leads to nerve cell damage, which results in intracellular dysfunction.

On the other hand, the peach tree is a Prunus species originated in China, and is a deciduous tree of 5-10 m height belonging to the genus Prunoideae, and the leaves are lanceolate 7-15 cm long and 2-3 cm wide. Flowers bloom in early spring before leaves and consist of 5 pink petals 2.5-3 cm in diameter. Fruits are juicy, reddish brown egg-shaped, with a relatively large seed, 1.5-2 cm in size, fleshy yellow or white, and its shell covered with fine hairs or smooth depending on the variety. Peach leaves have been used for arthritis and headaches as a traditional medicine ( Oxford English Dictionary ), and it has not been reported to protect neurons.

The present inventors have completed the present invention, confirming that the peach leaf extract exhibits neuronal cell protection while studying a micromaterial having various physiological activities from edible biological resources.

It is an object of the present invention to develop a substance that exhibits neuronal cell protection from edible biological resources and to provide a neuronal cell protective composition comprising the same.

It is also an object of the present invention to provide a method for purifying the neuronal protective substance from edible biological resources.

The present invention provides a nerve cell protective composition comprising a peach leaf extract as an active ingredient.

The present invention also provides a method for purifying the peach leaf extract fraction.

The present invention provides a neuronal protective use of the peach leaf extract. Therefore, the composition comprising the peach leaf extract as an active ingredient, cerebral neurological disease, stroke, cerebral infarction, cerebral embolism, ischemic neurological disease, degenerative neurological disease, stroke, Alzheimer's disease, Parkinson's disease, dementia, Huntington's disease, Peak disease associated with neuronal cell protection , Creutzfeldt-Jakob disease, multiple sclerosis, ischemic brain disease, epilepsy, Lou Gehrig's disease, memory loss, nerve cell damage caused by trauma or disease, concussion, progressive nuclear palsy, polypharyngeal atrophy, olive nucleus-brain-cerebellar atrophy (OPCA) Various diseases such as Shy-Dragger syndrome, stromal-black degeneration, Amyotrophic lateral sclerosis (ALS), essential tremor, cortical-basal ganglia degeneration, diffuse Lewis body disease, Parkinson-ALS-dementia complications, pick disease It can be used to effectively prevent or treat

The present invention provides a nerve cell protective composition comprising a peach leaf extract as an active ingredient.

In the preparation of the peach leaf extract of the present invention, dried or undried peach leaves or mixtures thereof can be used. For the efficiency of extraction the peach leaves can be used by crushing. In addition, in order to obtain an effective neuronal protective peach leaf extract, it may be subjected to a process of incubating for about 5 minutes at a high temperature of about 100 ℃ before pulverizing the peach leaf to inactivate the enzyme in vivo cells.

For the preparation of the peach leaf extract can be extracted according to a conventional extraction method using 3 to 10 times the extraction solvent of the peach leaf.

The extraction solvent may be extracted using water, an organic solvent, or a mixed solvent thereof which is widely used in natural product extraction. The organic solvent includes methanol or ethanol. In this case, methanol or ethanol may be mixed with water, but may be used without mixing with water for easy elution of the active ingredient. In one embodiment, the peach leaf extract may be a methanol extract.

The peach leaf extract may be further fractionated using an organic solvent that is different in polarity from the organic solvent used in the first extraction. In addition, fractions further purified may be obtained using various chromatography such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography, and the like. In the present invention, the peach leaf extract includes all the extracts, fractions and purified products obtained in each step of extraction, fractionation or purification as described above, their dilutions, concentrates or dried products.

Therefore, in one embodiment of the present invention, the peach leaf extract is extracted with peach leaves with water, an organic solvent or a mixed solvent, the residue is sequentially fractionated using hexane, chloroform and ethyl acetate and concentrated under reduced pressure It may be a peach leaf extract fraction obtained by drying. In one aspect, the peach leaf extract fraction may be an ethyl acetate fraction obtained by sequentially extracting the peach leaves with methanol and the residues sequentially using hexane, chloroform and ethyl acetate.

In another embodiment of the present invention, the peach leaf extract is extracted with peach leaves with water, an organic solvent or a mixed solvent thereof, the residue is sequentially fractionated using hexane, chloroforum and ethyl acetate, concentrated under reduced pressure and dried Chloroform in the fraction obtained by silica gel column chromatography using a mixed solvent of chloroform and methanol as a developing solvent with respect to the peach leaf extract fraction obtained by using the solvent as the developing solvent of methanol: 90:10 to 70:30. It may be a peach leaf extract fraction obtained under the conditions.

In another embodiment of the present invention, the peach leaf extract is extracted with peach leaves with water, an organic solvent or a mixed solvent thereof, the residue is sequentially fractionated using hexane, chloroforum and ethyl acetate and concentrated under reduced pressure Chloroform in the fraction obtained from the dried peach leaf extract fraction obtained by performing silica gel column chromatography using a mixed solvent of chloroform and methanol as a developing solvent: development of methanol: 90:10 to 70:30 The peach leaf extract fraction may be obtained under solvent conditions, and the peach leaf extract fraction may be further purified by performing thin layer chromatography (TLC) or high performance liquid chromatography (HPLC).

The invention also

1) extracting the peach leaves with water, an organic solvent or a mixed solvent thereof, and fractions were extracted sequentially with hexane, chloroforum and ethyl acetate, concentrated under reduced pressure and dried to obtain a peach leaf extract fraction;

2) Chloroform in the fraction obtained by silica gel column chromatography using a mixed solvent of chloroform and methanol as a developing solvent for the peach leaf extract fraction obtained in step 1: methanol = 90:10 to 70 Obtaining a peach leaf extract fraction under a developing solvent condition of: 30; And

3) performing a thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) on the peach leaf extract fraction obtained from step 2 to obtain a peach leaf extract fraction exhibiting neuronal cell protective activity.

Provided is a method for purifying the peach leaf extract fraction for protecting nerve cells.

In the following examples of the present invention, the residue obtained by extracting peach leaves with 100% methanol (hereinafter referred to as 'CM'), fractions in the order of hexane, chloroform, ethyl acetate and dried under reduced pressure to extract peach leaves Fractions (hereinafter referred to as' MH ',' MC ',' ME and 'MM' in the Examples) were prepared. In addition, the ethyl acetate fraction (ME) in the peach leaf extract fractions were further fractionated and purified. The peach leaf extract of the present invention obtained through this process has a protective effect of increasing the survival rate of neurons up to about 8 times as compared with the positive control treated with only SIN-1 (3-morpholinosydninimine hydrochloride), a peroxynitrite generating reagent. Indicates.

 Therefore, the peach leaf extract of the present invention may be included as an active ingredient of the neuronal protective composition.

The neuronal protective composition of the present invention can be used as a composition for the prevention or treatment of diseases related to the protection of neurons. Diseases related to the protection of neurons are not limited to, for example, cranial nerve disease, stroke, cerebral infarction, cerebral embolism, ischemic neurological disease, degenerative neurological disease, stroke, Alzheimer's disease, Parkinson's disease, dementia, Huntington's disease, Peak disease, Creutzfeldt-Jakob disease, multiple sclerosis, ischemic brain disease, epilepsy, Lou Gehrig's disease, memory loss, nerve cell damage caused by trauma or disease, concussion, progressive nuclear palsy, polypharyngeal atrophy, olive nucleus-brain-cerebellar atrophy (OPCA), Group consisting of Shy-Dragger syndrome, stromal-black degeneration, Amyotrophic lateral sclerosis (ALS), essential tremor, cortical-basal ganglia degeneration, diffuse Lewis body disease, Parkinson-ALS-dementia complications, pick disease It may be a disease selected from.

The composition can be used with a peach leaf extract of the present invention as well as a neuroprotective agent known in the art.

 The neuronal cell protective composition according to the present invention may be a pharmaceutical composition or a food composition.

A pharmaceutical composition comprising the peach leaf extract of the present invention as an active ingredient may be prepared using a pharmaceutically acceptable and physiologically acceptable adjuvant in addition to the active ingredient, and the adjuvant may include excipients, disintegrants, sweeteners, Binders, coatings, swelling agents, lubricants, lubricants, flavoring agents, and the like can be used.

The pharmaceutical composition may be preferably formulated into a pharmaceutical composition comprising one or more pharmaceutically acceptable carriers in addition to the active ingredient described above for administration.

Formulation forms of the pharmaceutical composition may be granules, powders, tablets, coated tablets, capsules, suppositories, solutions, syrups, juices, suspensions, emulsions, drops or injectable solutions. For example, for formulation in the form of tablets or capsules, the active ingredient may be combined with an oral, nontoxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water and the like. In addition, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included in the mixture. Suitable binders include but are not limited to natural sugars such as starch, gelatin, glucose or beta-lactose, natural and synthetic gums such as corn sweeteners, acacia, trackercance or sodium oleate, sodium stearate, magnesium stearate, Sodium benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum, and the like. Acceptable pharmaceutical carriers in compositions formulated in liquid solutions are sterile and physiologically compatible, including saline, sterile water, Ringer's solution, buffered saline, albumin injectable solutions, dextrose solution, maltodextrin solution, glycerol, ethanol and One or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers and bacteriostatic agents may be added as necessary. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into injectable solutions, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like. Furthermore, the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA can be formulated according to each disease or component, as appropriate in the art.

The present invention also provides a food composition for nerve cell protection comprising the peach leaf extract.

The food composition according to the present invention may be formulated in the same manner as the pharmaceutical composition, used as a functional food, or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, meat, chocolate, foods, confectionery, pizza, ramen, other noodles, gums, ice creams, alcoholic beverages, vitamin complexes, and health supplements. .

The present invention provides a health functional food comprising the neuron protective composition.

The present invention also provides the use of a composition comprising the peach leaf extract as an active ingredient for the manufacture of a medicament or food for protecting neurons. The composition of the present invention comprising the peach leaf extract as an active ingredient can be used for the manufacture of a medicament or food for the prevention or treatment of diseases related to neuronal cell protection.

The present invention also provides a method of neuronal cell protection comprising administering to a mammal a therapeutically effective amount of a peach leaf extract, or a method of preventing or treating a disease associated with neuronal cell protection.

As used herein, the term "mammal" refers to a mammal that is the subject of treatment, observation or experimentation, preferably human.

As used herein, the term “therapeutically effective amount” means an amount of an active ingredient or pharmaceutical composition that induces a biological or medical response in a tissue system, animal or human, as contemplated by a researcher, veterinarian, doctor or other clinician, This includes amounts that induce alleviation of the symptoms of the disease or disorder being treated. It will be apparent to those skilled in the art that the therapeutically effective dosages and frequency of administrations for the active ingredients of the present invention will vary depending on the desired effect. Therefore, the optimal dosage to be administered can be readily determined by one skilled in the art and includes the type of disease, the severity of the disease, the amount of active ingredients and other ingredients contained in the composition, the type of formulation, and the age, weight, general health of the patient. It can be adjusted according to various factors, including the condition, sex and diet, time of administration, route of administration and rate of composition, duration of treatment, and drugs used simultaneously. In the treatment method of the present invention, for adults, when the peach leaf extract of the present invention is administered once to several times a day, it is preferable to administer at a dose of 1 mg / kg to 250 mg / kg.

In the treatment method of the present invention, the composition comprising the peach leaf extract of the present invention as an active ingredient may be administered by oral, rectal, intravenous, intraarterial, intraperitoneal, intramuscular, intrasternal, transdermal, topical, intraocular or intradermal routes. It may be administered in a conventional manner.

Advantages and features of the present invention and methods for achieving them will be apparent with reference to the embodiments described below in detail. However, the present invention is not limited to the embodiments disclosed below, but may be implemented in various forms. It is provided to fully convey the scope of the invention to those skilled in the art, and the present invention is defined only by the scope of the claims.

EXAMPLE

Example 1 Preparation of Peach Leaf Extract and Peach Leaf Extract Fractions and Confirmation of Their Neuronal Protective Activity

Peach leaves were incubated at 100 ° C. for 5 minutes to inactivate enzymes in living cells and finely pulverized with a hand mixer, and then allowed to stand for 24 hours with 5 times 100% methanol (v / v) of the sample at room temperature. The extract was extracted, and the extract was concentrated under reduced pressure and dried ('CM'). The dried product was dissolved in 1 liter of distilled water and fractionated using the same amount of hexane, chloroform and ethyl acetate in the order of fraction funnel ('MH', 'MC', 'ME), and the remaining was dissolved in methanol and filtered through a filter cloth. The residue was concentrated under reduced pressure and dried to obtain an extract ('MM'). 1 is a process chart showing the methanol extraction of the peach leaf and the organic solvent fraction step of the extract according to an embodiment of the present invention.

In order to confirm the neuroprotective activity of peach leaf extract and each fraction, we established an in vitro assay system using SIN-1, a generation agent of peroxynitrite, and SH-SY5Y cell, a fibroblastoma cell line, to improve cell viability. Measured. First, SH-SY5Y cells were seeded by 3 X10 ⁴ cells in 96 well dishes, and then treated with 1 mg / mL extracts library sample and 250 μM SIN-1 for 24 hours. To measure formazan formation, 25 mg of 5 mg / ml 3- (4,5-dimethylthiazoyl-2-yl) -2,5-diphenyltetrazolium bromide (MTT) solution was added and reacted for 3 hours, and then the medium was removed. The resulting formazan was dissolved in DMSO to measure A ₅₄₀ and survival rate was measured.

As a result of measuring the neuronal protective activity according to the fraction as described above, the first showed a high activity even in the methanol extract (CM), it was confirmed that the highest activity in the ethyl acetate fraction (ME). 2 is a graph showing the results of neuronal cell protective activity of each step fraction of FIG.

Example 2 Purification of Peach Leaf Extract Fraction by Chromatography and Confirmation of Neuronal Protective Activity of Peach Leaf Extract Fraction

The peach leaf ethyl acetate fraction (ME) obtained through Example 2 was purified using chromatography. Ethyl acetate fraction (ME) was loaded onto a silica gel 60 column activated with chloroform, and 5 bed volumes of chloroform and methanol (CHCl ₃ : MeOH = 100: 0 ~ 0: 100) mixed solution were developed. The neuronal protective activity against the light was examined, and finally, a small fraction having neuronal protective activity was isolated from the first chloroform-methanol 80:20 fraction (CME3a).

TLC was performed on the elution fraction (CME3a) having high neuronal cell protective activity isolated as described above. In TLC, the active fractions were separated using ethyl acetate / formic acid / D.W (65:15:20, v / v / v) as the developing solvent.

The high activity fraction (CME3aβ) obtained from TLC was collected using HPLC (u-Bondapak C ₁₈ (4.5 × 150 mm) and H ₂ O: acetonitrile = 100: 0 ~ 40: 60 solvent conditions and 1 ml / min After eluting at a flow rate of and concentrating only the active fraction (CME3aβ-III), the active fraction was subjected to HPLC under the same conditions. Process diagram showing the purification step of the ethyl acetate fraction (CME) of the peach leaf methanol extract obtained from Example 1, Figure 4 is an HPLC chromatogram showing the purification degree of the separation material of FIG.

Example 3 Evaluation of Neuronal Protective Activity According to Purification

Using the method of Example 1, the neuronal protective activity of each of the purified substances separated from the peach leaves was measured. Figure 2 is a chart showing the results of testing the inhibitory activity of the purification step material. As the purification step proceeded, more inhibitory activity was shown, and the final HPLC purified product (CME3aβ-III) showed the highest inhibitory activity at the concentration of 0.2 mg / ml.

1 is a process chart showing the methanol extraction of the peach leaf and the organic solvent fraction step of the extract according to an embodiment of the present invention.

2 is a graph showing the results of neuronal cell protective activity of each step fraction of FIG.

Figure 3 is a graph showing the neuronal protective activity of the peach leaf methanol extract (CM-M) obtained by further purification of the methanol extract (CM) of the peach leaf using an organic solvent.

Figure 3 is a process showing the purification step of the ethyl acetate fraction (CME) of the peach leaf methanol extract obtained from Example 1.

FIG. 4 is an HPLC chromatogram showing purification of the separation material of FIG. 3.

5 is a graph showing the neuronal protective activity according to the purification step of the peach leaf extract fraction.

Claims (10)

Neuronal cell protective composition comprising a peach leaf extract as an active ingredient. The method of claim 1, The peach leaf extract is a nerve, protective composition for water, an organic solvent or a mixed solvent extract thereof. The method of claim 2, The peach leaf extract is a nerve extract protective composition of methanol extract. The method of claim 1, The peach leaf extract is a peach leaf extract obtained by extracting the peach leaf with water, an organic solvent or a mixed solvent thereof, sequentially fractionating the residue using hexane, chloroforum and ethyl acetate, and concentrating under reduced pressure and drying. Cell protective composition. The method of claim 4, wherein The peach leaf extract is a peach leaf extract with methanol, the residue is sequentially fractionated using hexane, chloroforum and ethyl acetate, concentrated under reduced pressure and dried to obtain a ethyl acetate fraction of the peach leaf methanol extract obtained by drying. The method of claim 1, The peach leaf extract is extracted from the peach leaf with water, an organic solvent or a mixed solvent thereof, and the residue is sequentially fractionated using hexane, chloroforum and ethyl acetate, concentrated under reduced pressure, and dried. Chloroform in the fraction obtained by silica gel column chromatography using a mixed solvent of chloroform and methanol as a developing solvent: the peach leaf extract fraction obtained under the developing solvent conditions of 90:10 to 70:30. Neuronal cell protective composition. The method of claim 1, The peach leaf extract is a peach leaf extract obtained by extracting the peach leaf with water, an organic solvent or a mixed solvent thereof, sequentially fractionating the residue using hexane, chloroforum and ethyl acetate, and concentrating the remaining residue under reduced pressure and drying. Chloroform in the fraction obtained by silica gel column chromatography using a mixed solvent of chloroform and methanol as a developing solvent: Peach leaf extract under the developing solvent condition of methanol = 90:10 ~ 70:30 Obtaining a fraction, the composition for nerve cell protection comprising a peach leaf extract fraction further purified by performing TLC (thin layer chromatography) or HPLC (high performance liquid chromatography) on the obtained peach leaf extract fraction as an active ingredient. The method according to claim 1, wherein The composition is a cerebral nerve disease, stroke, cerebral infarction, cerebral embolism, ischemic neurological disease, degenerative neurological disease, stroke, Alzheimer's disease, Parkinson's disease, dementia, Huntington's disease, Peak disease, Creutzfeldt-Jakob disease, multiple sclerosis, ischemic brain disease, epilepsy, Lou Gehrig's disease Nerve cell damage due to illness, memory loss, trauma or disease, concussion, progressive nuclear palsy, multiple system atrophy, olive nucleus-brain-cerebellar atrophy (OPCA), Shire-Dragger syndrome, primitive-black degeneration, muscular dystrophy Neurons used for the prophylaxis or treatment of diseases selected from the group consisting of lateral sclerosis (ALS), essential tremor, cortical-basal nucleus degeneration, diffuse Lewis body disease, Parkinson-ALS-dementia complications, and pick disease Protective composition. Health functional food comprising a composition for protecting neurons according to claim 1. 1) extracting the peach leaves with water, an organic solvent or a mixed solvent thereof, and fractions were extracted sequentially with hexane, chloroforum and ethyl acetate, concentrated under reduced pressure and dried to obtain a peach leaf extract fraction; 2) Chloroform in the fraction obtained by silica gel column chromatography using a mixed solvent of chloroform and methanol as a developing solvent for the peach leaf extract fraction obtained in step 1: methanol = 90:10 to 70 Obtaining a peach leaf extract fraction under a developing solvent condition of: 30; And 3) performing a thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) on the peach leaf extract fraction obtained from step 2 to obtain a peach leaf extract fraction exhibiting neuronal protective activity. Method for Purifying Peach Leaf Extract Fraction for Neuronal Cell Protection.

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