KR20100057234A - Method, bio disk, and system for measuring blood dynamic information - Google Patents

Abstract

PURPOSE: A disk and system for measuring blood change information are provided to easily measure with little blood amount at home or clinical place and to diagnose and treat cardiovascular diseases. CONSTITUTION: A biodisk(100) comprises a diagnosis channel(200). The diagnosis channel has a blood loading chamber(210) on which anticoagulant is coated, a flow path chamber(220) having blood flow path through the loading channel, and a valve(230) between the loading chamber and flow path chamber. A system for measuring blood change information comprises: the disk; a driving unit for driving the disk using the rotation force; and a measuring unit for measuring sample information.

Description

Bio disk and measuring system for measuring blood rheological information and method thereof {Method, bio disk, and system for measuring blood dynamic information}

The present invention relates to a bio-disc and a measurement system for measuring blood rheological information, and a method thereof. A bio-disc and measurement for measuring blood rheological information that can be used as an index for diagnosis and treatment of cardiovascular diseases by using a disk and an optical system. A system and method thereof are provided.

Blood consists of tangible components (red blood cells, white blood cells, and platelets) and intangible components (plasma), and red blood cells make up a significant portion (about 40 to 50%) of the total blood.

As shown in Figure 1, by the difference in specific gravity, tangible components in the blood will sink down after a long time. Blood, which occupies 4 to 5 L in the human body, serves to transport oxygen and nutrients to tissues and also to collect waste products.

In the body, 4 to 5 L of blood undergoes repetitive flow, and blood flow in blood vessels is very important not only in large blood vessels such as aorta, but also in microvascular vessels.

Due to the recent westernization of eating habits, the prevalence of cardiovascular diseases such as hypertension, arteriosclerosis, and myocardial infarction is gradually increasing, and the direct and indirect prevention / treatment indexes of cardiovascular diseases have also become many issues.

Therefore, there is a need for a system for measuring / analyzing blood rheological information that easily determines blood flow characteristics in blood vessels at the treatment site or at home. However, until now, only ESR or blood coagulation capacity has been measured in the laboratory of a large hospital, which is also measured only for limited diseases.

The technical problem to be achieved by the present invention is to significantly reduce the amount of blood samples and the time required by using a CD / DVD disk, blood rheological information such as Erythrocyte Sedimentation Rate (Erythrocyte Sedimentation Rate) and Erythrocyte Aggregation (Erythrocyte Aggregation) It is an object of the present invention to provide a bio-disc and a measurement system and method for measuring blood flow rate information that can facilitate measurement.

As a first aspect for achieving the above technical problem, the present invention, in the bio-disc, comprising at least one diagnostic channel, the diagnostic channel, the blood loading chamber is coated with an anticoagulant formed inlet; And a flow path chamber forming a blood flow path through which the blood introduced through the loading chamber is moved.

As a second aspect for achieving the above technical problem, the present invention, blood rheology information measuring system, the anticoagulant is coated on at least a portion including a diagnostic channel capable of loading blood; A drive unit for driving the disk using a rotational force; And a measuring unit capable of measuring information of the sample in the diagnostic channel of the disk.

As a third aspect for achieving the above technical problem, the present invention comprises the steps of reacting the blood with the anticoagulant using a diagnostic channel coated with the anticoagulant is injected blood; Rotating the bio-disc at a first rotational speed that can pass through the valve; And rotating the bio-disc at a second rotational speed, and optically measuring the position of the interface between the settled blood cells and the plasma using the light emitting unit and the light receiving unit.

The present invention can easily measure blood flow rate information such as erythrocyte sedimentation rate and erythrocyte agglutination ability, which are measured only in the diagnostic examination medicine of a large hospital, at home or on-site, with low blood volume and measurement time. It can be used as a prophylactic and therapeutic index.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

While the invention allows for various modifications and variations, specific embodiments thereof are illustrated by way of example in the drawings and will be described in detail below. However, it is not intended to be exhaustive or to limit the invention to the precise forms disclosed, but rather the invention includes all modifications, equivalents, and alternatives consistent with the spirit of the invention as defined by the claims.

When an element, such as a layer, an area, is mentioned as being on another component "on", it will be understood that it may be directly on another element or there may be an intermediate element in between.

The terms first, second, etc. may be used to describe various elements, components, regions, layers, and / or regions, but such elements, components, regions, layers, and / or regions It will be understood that it should not be limited by these terms.

Dividing the blood in order of density, usually divided into plasma, middle layer, red blood cells, etc. The erythrocyte sedimentation rate is determined using the Westergren method recommended by the International Commission on Standards for Clinical Testing. In this method, 1 ml of blood is filled into a 30 cm long glass tube with a diameter of 2.5 mm to measure the degree of erythrocyte sedimentation after 60 minutes.

The present invention aims to provide a bio-disc and diagnostic device that can easily measure blood rheological information such as erythrocyte sedimentation rate and hemagglutination ability, which are important indices of blood flow, in the home or on-site.

Optical disks, driven by optical drives, can rotate at high speeds and control the speed and direction of rotation of these disks, so the disks have the right channels to receive blood or other fluids for diagnostic purposes. If it is provided, it can be used as a bio-disc using the centrifugation function by the rotation of the disc.

In general, as shown in Figure 1, if the blood containing the anticoagulant for a long time, the red blood cells are settled by the difference in specific gravity.

This erythrocyte sedimentation occurs in three stages. In the first stage, sedimentation rarely occurs during the initial 10 minutes, and time for erythrocyte erosion and aggregation is formed.

The second stage is the time when red blood cell sedimentation takes place at a constant rate for the next 40 minutes. The third stage is the last 10 minutes, when the erythrocyte sedimentation slows down and the red blood cells are filled in the lower part of the test tube. 2 shows a schematic diagram in which the ectopic phenomenon of erythrocytes separated from each other occurs.

As such, gravity (g) acts to measure blood flow rate information such as erythrocyte sedimentation rate, and the gravity time can be shortened by using the rotational force at low RPM of an optical drive such as a CD / DVD ROM. .

As shown in FIG. 3, a system capable of measuring blood flow rate information using an optical drive includes a bio disc 100 including a diagnostic channel 200 capable of loading blood, and a rotation force. The driving unit 400 for driving the disk 100 and the measurement unit 300 for measuring a phenomenon occurring in the diagnostic channel 200 of the disk 100 may be configured.

As shown in FIG. 4, the diagnostic channel 200 included in the bio disc 100 includes a blood loading chamber 210 coated with an anticoagulant such as sodium citrate, and the loading chamber 210. It includes a flow path chamber 220 for forming a blood flow path through which the blood flowed through.

In this case, a valve 230 such as a hydrophobic barrier may be positioned between the loading chamber 210 and the flow path chamber 220 to prevent the movement of the blood sample by using a capillary phenomenon.

Such a valve 230 may be omitted by designing a passive valve system through an optimal flow path design.

An inlet may be formed in the loading chamber 210, and the flow path chamber 220 may be formed of a glass tube that can be separated and mounted.

On the other hand, the peripheral portion 110 of the bio-disc 100 may include bar code disc information. The disk information may include various information for driving and reading the disk 100 such as the position of the diagnostic channel 200 of the disk 100.

The measurement unit 300 may use an optical drive, the light emitting unit 310 for emitting light having a wavelength band of 785 nm or 650 nm using an optical system used in the current CD / DVD system, and the light emitting unit The light emitted from 310 may include a light receiver 320 for receiving light reflected or transmitted through the diagnostic channel 200 of the disk 100.

Here, the light emitting unit 310 and / or may further include a lens 330 located in the light receiving unit 320, it may include a transfer unit 340 for transferring such a measuring unit 300.

Referring to the process of measuring the erythrocyte sedimentation rate using this measurement system as follows.

First, a certain amount of blood is administered to the blood loading chamber 210 of the disk according to the diameter and length of the flow path chamber 220. The amount of blood to be administered here is determined to be at least less than the volume of the flow path chamber 220.

Thereafter, the blood is reacted with the anticoagulant for a predetermined time, and then passed through the valve 230. In the case where the valve is a hydrophobic barrier, the bio disc 100 is rotated by the driving unit 400 at a speed w1 that can pass through the hydrophobic membrane.

The rotational speed of the bio disc 100 is directly related to the ESR measurement time. For example, the relationship between the revolutions per minute (rpm) and the gravitational acceleration (g) acting on the disk is directly related to the length of the flow path chamber 220.

Where m is mass, r is rotation radius, and ω is angular velocity.

In the relationship of Equation 1, it is possible to determine the rotational speed corresponding to the multiple of the earth's gravity. For example, the rotational speed w2 corresponding to 12 times the earth's gravity can be determined. Through this, by increasing the acceleration of gravity (g) to 12 times, the time for measuring the erythrocyte sedimentation rate may be reduced from 60 minutes to 5 minutes, and the sample amount may be minimized according to the diameter and length of the flow path chamber 220.

After the rotation for 5 minutes at the optimum rotation speed (w2), the position of the interface between the settled blood cells and the plasma is optically measured while moving the light emitting unit 310 and the light receiving unit 320 along the transfer unit 340.

On the other hand, erythrocyte sedimentation rate is closely related to hematocrit, which is the volume ratio of erythrocytes in the blood. Therefore, the hematocrit can be calculated by increasing the rotational speed of the disk 100 (w3) and measuring optically the position of the interface between the red blood cells and the plasma after a certain time.

Unlike the method of measuring erythrocyte sedimentation rate, erythrocytes in the blood without flow cause a tendon phenomenon as shown in FIG.

When the light received by the light receiving unit 320 at a specific position is quantified with time at a very low rotation speed of the disk 100, a graph as shown in FIG. 6 may be obtained.

That is, the erythrocyte sedimentation rate may be divided into a fast section (f1) that linearly descends among the erythrocyte aggregation in the blood, and a slow section (f2) where aggregation occurs in 3D and in 3D. .

By using such a bio-disc and a measurement system, the erythrocyte sedimentation rate and hemagglutination ability, which are important indexes of blood flow, can be easily measured at home or on-site to be used for diagnosis of various diseases such as immune diseases, inflammatory diseases and cardiovascular diseases. It can be.

The above embodiment is an example for explaining the technical idea of the present invention in detail, and the present invention is not limited to the above embodiment, various modifications are possible, and various embodiments of the technical idea are all protected by the present invention. It belongs to the scope.

1 is a schematic diagram showing the configuration of blood.

2 is a schematic diagram illustrating the stage of aggregation of erythrocytes in the blood.

3 is a cross-sectional view illustrating an embodiment of a blood rheological information measuring system.

4 is a plan view showing an example of a bio-disc.

5 is a cross-sectional view showing an example of the measurement unit.

6 is a graph showing the aggregation ability of erythrocytes in the blood measured.

Claims (12)

In the bio disc, At least one diagnostic channel, wherein the diagnostic channel, A blood loading chamber coated with an anticoagulant and having an inlet formed therein; And a flow path chamber forming a blood flow path through which the blood introduced through the loading chamber is moved. The method of claim 1, wherein the diagnostic channel, And a valve positioned between the loading chamber and the flow passage chamber. The bio-disc according to claim 2, wherein the valve is a capillary tube. The bio-disc according to claim 2, wherein the valve is a hydrophobic membrane for preventing the movement of blood samples. The bio-disc of claim 1, wherein the flow path chamber is detachable and mounted. In the blood rheological information measurement system, A disk comprising a diagnostic channel coated with at least a portion of an anticoagulant to load blood; A drive unit for driving the disk using a rotational force; And a measurement unit capable of measuring information of a sample in a diagnostic channel of the disk. The method of claim 6, wherein the measuring unit, A light emitting unit for emitting light; And a light receiver configured to receive light reflected or transmitted through the diagnostic channel of the disc by the light emitted from the light emitter. The method of claim 7, wherein the measuring unit, A lens positioned on at least one of the light emitting unit and the light receiving unit; And a transfer unit capable of transferring the light emitting unit and the light receiving unit. The method of claim 6, wherein the diagnostic channel of the disk, A blood loading chamber coated with an anticoagulant and having an inlet formed therein; A flow path chamber forming a blood flow path through which the blood introduced through the loading chamber is moved; And a valve positioned between the loading chamber and the flow passage chamber. In the method of measuring blood rheology information using the system of claim 9, Reacting the blood with the anticoagulant using a diagnostic channel coated with the anticoagulant infused with the blood; Rotating the bio-disc at a first rotational speed that can pass through the valve; And rotating the bio-disc at a second rotational speed, and optically measuring the position of the interface between the settled blood cells and the plasma using a light emitting unit and a light receiving unit. . The method of claim 10, wherein the second rotation speed is a rotation speed corresponding to 12 times the earth's gravity. The method of claim 10, further comprising optically measuring a position of an interface between red blood cells and plasma after increasing the rotation speed of the disk to a third rotation speed.

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