KR20100035199A - Apparatus for bio diagnosis of rotate type - Google Patents

Abstract

PURPOSE: A rotation type bio diagnostic apparatus is provided to measure a plurality of bio materials by installing at least one sensing material coated with a magnetic component in a rotation type disc through one time measurement. CONSTITUTION: A rotation type bio diagnostic apparatus comprises a rotation unit(30) mounting a sensing material(40) in a rotation type disc; a signal sensing unit(10) having a magnetic resistance sensor(12); and a monitoring unit(20) for monitoring a signal measured through the signal sensing unit.

Description

Apparatus for bio diagnosis of rotate type

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a bio-diagnostic device, and more particularly, to measure a plurality of bio-materials through a single measurement by mounting one or more sensing materials, which are magnetic materials coated bio-materials (antigens or specimens), onto a rotating disk. It relates to a rotation type bio-diagnostic device adapted to.

In general, a magnetic resistance sensor (magnetic sensor) is a sensor for measuring the size and direction of the magnetic field or the line of magnetic force, the magnetic field is measured by changing the properties of various materials due to the influence of the magnetic field. Hall elements or magnetoresistive (MR) elements are made using the Hall effect, magnetoresistance effect, etc., and they are also used to manufacture VTRs (Video Tape Recorders) and tape recorders.

Meanwhile, a large magnetoresistive sensor cartridge and a sensing cell array (Korean Patent Laid-Open Publication No. 2004-55387, Japanese Patent Laid-Open Publication No. 2004-205495) have been disclosed as blood component analysis means.

1 is a conceptual diagram of a conventional giant magnetoresistive sensor and a sensing cell array using the same.

Thus, a plurality of giant magnetoresistive sensor cartridges are placed in a sensing cell array consisting of N columns and M rows, and a biosensor cartridge chip consisting of the sensing cell array is prepared at the package or wafer level.

Component measurement data consisting of surrounding materials are then exposed to each large magnetoresistive sensor cartridge. Then, the respective component measurement data are measured in the sensing cell array of the giant magnetoresistive sensor cartridge, and the component data measured by the blood component analyzer is electrically analyzed.

The existing invention relates to a giant magnetoresistive sensor cartridge and a sensing cell array using the same. A technology for sensing and analyzing components of peripheral materials each consisting of a plurality of components according to the detection of different magnetic fields to separate and analyze them into electrical components Started.

Existing invention has a large magnetoresistive sensor comprising a sensing cell array Giant Magneto Resistance (GMR) element, a switching element, and a magnetic material (or forcing word line) having a plurality of row and column shapes on a biosensor cartridge chip. Arranged in a sensing cell array, the magnetic susceptibility of each of the peripheral materials having different characteristics is sensed, and the electrical components of the peripheral materials are analyzed.

Alternatively, the peripheral material composed of a plurality of different components may be separated and analyzed into electrical components according to susceptibility or permittivity.

Magnetized pair sensing sensor with MTJ (Magnetic Tunnel Junction) or GMR element, magnetoresistive sensor with MTJ element and magnetic material, dielectric constant sensor with sensing capacitor switching element, MTJ element or GMR element, current line, variable ferromagnetic layer and Magnetization Hall sensing sensors with switching elements, or large magnetoresistive (GMR) sensors composed of GMR elements, switching elements, and magnetic materials, are arranged in a sensing cell array having a plurality of row and column shapes at the biosensor tip, each having different characteristics. According to the component of the peripheral material and the size of the component that indicates the different susceptibility or dielectric constant, respectively, and the components of the peripheral material to be analyzed to be separated into electrical components.

The existing technology described the use of sensors as arrays and the sensing mechanism between sensors and bio-contents. The emphasis was placed on circuit configuration in relation to array types.

However, this sensor configuration of the existing technology is based on a complicated memory structure, and has a big problem in producing a simple sensing kit (Kit). In addition, there is a cost disadvantage in that the sensor and circuit stages using a semiconductor process such as an MRAM (Magnetic Random Access Memory) structure have to be composed of a Complementary Metal Oxide Semiconductor (CMOS) switching circuit.

Accordingly, the present invention has been proposed to solve the above conventional problems, and an object of the present invention is to mount at least one sensing material, which is a biomaterial (antigen or sample) coated with a magnetic component, on a disk of a rotating type. To provide a rotation type bio-diagnostic apparatus that can measure a plurality of bio materials through a single measurement.

Figure 2 is a block diagram of a rotation type bio diagnostic apparatus according to an embodiment of the present invention.

As shown therein, the rotating part 30 for mounting the sensing material 30 to the rotating disk 31; A signal sensing unit 10 having a GMR sensor 12 measuring the sensing material 40 mounted on the rotating unit 30; And a monitoring unit 20 for monitoring the signal measured by the signal detecting unit 10.

As shown in FIG. 6, the rotating part 30 includes a disk 31 of a rotating type and on which a sensing material 40 is mounted; A support part 32; One side is attached to the support part 32, the other side is connected to and fixed to the disk 31, and the front and rear moving parts 33 which allow the disk 31 to move in the front and rear directions; One side is attached to the support 32, the other side is fixed to the signal detecting unit 10, the vertical detecting unit 35 to move the signal detecting unit 10 in the vertical direction; configured to include It is characterized by.

The rotating unit 30, the disk 31 is connected to the front and rear moving unit 33, the rotating unit 34 to rotate the disk 31; characterized in that it further comprises a configuration.

The signal detection unit 10, as shown in Figure 5, the substrate (11); A magnetoresistance (MR) sensor 12 mounted on the substrate 11 and outputting a detection signal when sensing the sensing material 40; An electrode pattern 15 having electrodes for transmitting a detection signal of the magnetoresistive (MR) sensor 12 to the monitoring unit 20; Wire bonding (16) connecting the electrode of the electrode pattern (15) and the magnetoresistive (MR) sensor (12); It characterized in that it comprises a; epoxy molding 17 to protect the connection portion of the wire bonding 16.

The signal detecting unit 10 further includes holes 13 and 14 formed in the substrate 11 such that the magnetoresistive MR sensor 12 is mounted on the substrate 11. do.

The signal detection unit 10 may be configured to output a detection signal by applying a constant frequency to the magnetoresistive MR sensor 12.

The rotation type biodiagnostic apparatus according to the present invention can overcome the detection range limited to the size of the sensing element by mounting at least one sensing material, which is a magnetic substance coated biomaterial (antigen or sample), on the rotation type disk. In addition, noise can be distinguished between frequencies by a dynamic scanning method, thereby enabling quantitative measurement analysis. In addition, many cartridges, membranes, and antibodies that are currently in use can be installed directly on the disk surface, which can analyze multiple sensing substances (antigens or samples) with a single scan.

Referring to the preferred embodiment of the rotation type bio diagnostic apparatus according to the present invention configured as described above in detail with reference to the accompanying drawings as follows. In the following description of the present invention, detailed descriptions of well-known functions or configurations will be omitted if it is determined that the detailed description of the present invention may unnecessarily obscure the subject matter of the present invention. It is to be understood that the following terms are defined in consideration of the functions of the present invention, and may be changed according to the intention of the user, the operator, or the precedent, and the meaning of each term should be interpreted based on the contents will be.

First, the present invention is to apply a magnetoresistance (MR) chip used in the existing MRAM as a biosensor, bio-in the cartridge or membrane for POCT (Point of Care Testing) commonly used for the measurement of biomaterials (antigens or samples) The present invention is to develop a device that can mount magnetic particles to be used as a medium (antigen or sample) and effectively detect magnetic signals generated at that time.

In addition, in order to establish a multiple detection system that can detect one or more biomaterials (antigens or samples) through one measurement, a disk type device was developed to create a bio-type using a large magnetoresistance. We want to develop a sensor.

Therefore, when a magnetoresistance sensor (Magneto Resistance) is installed in the signal detection unit for the biosensor, the magnetic material coated biomaterial (antibody or sample) is detected and sensed to be separated and analyzed as an electrical component.

According to the present invention, when a large magnetoresistive device manufactured by a conventional semiconductor unit process is applied as a biosensor, the sensing element and the biomaterial are directly contacted to improve the sensitivity of the small amount of the sensing material to perform quantitative analysis. Was intended. However, in the conventional method, the sensitivity itself can be improved to 1: 1 contact, but the capacitive control of the sensing material by the limited volume of the device is limited, and each device requires a separate CMOS circuit, thereby providing high cost and It will be difficult to reuse.

 Accordingly, the present invention intends to use a non-contact magnetoresistive sensor (MR) as a sensing material between sensors for biodiagnosis. Thus, by installing a cartridge or membrane used in the existing POCT to the diagnostic kit, the measuring instrument for effective cartridge measurement was developed. In addition, the present invention can overcome the detection range that was limited only to the size of the sensing element, can distinguish the noise (noise) between frequencies by the dynamic scanning method can be quantitative measurement analysis. In addition, many cartridges, membranes, and antibodies currently in use can be installed directly on the disk surface, allowing analysis of multiple sensing substances (antigens or samples) with a single scan.

In particular, the magnetoresistive sensor (MR) type that can be used in the present invention includes a normal magnetoresistance (OMR) sensor, anisotropic magnetoresistance (AMR) sensor, a giant magnetoresistance (GMR) sensor, It is preferable to use any one selected from a colossal magnetoresistance (CMR) sensor, a tunneling magnetoresistance (TMR) sensor, and a magnetic tunneling junction (MJT) sensor. Particularly preferably, a giant magnetoresistance (GMR) sensor may be used.

Briefly, for each sensor described above, in the case of a non-magnetic conductor and a semiconductor material, the resistance changes because the conduction electrons are subjected to Lorentz force when the magnetic field is applied from the outside. In general, there is a characteristic that the change of resistance is quite small.

In addition, the anisotropic magnetoresistance (AMR) sensor uses an anisotropic magnetoresistance. In other words, in ferromagnetic conductor materials, in addition to the general magnetoresistance, there exist easy and hard axes due to spin-orbit coupling (d-band splitting by Spin-Orbit coupling). This is called AMR (Anisotropic MR) because it is determined by the angle between the external magnetic field direction and the current direction and is determined by the direction. The sensor is characterized by a 2.5% difference in resistance in each of these directions.

In addition, a giant magnetoresistance (GMR) sensor may be used, and the giant magnetoresistance (GMR) sensor has a feature that has a magnetoresistance several times to several times larger than an anisotropic magnetoresistance material. In particular, the change in resistance is caused by additional scattering of conduction electrons according to the relative spin direction difference of adjacent magnetic layers, and its mechanism is fundamentally different from that of OMR or AMR.

In addition, the Colossal Magnetoresistance (CMR) sensor described above was first discovered by von Helmolt in 1993. The main characteristic is a sensor having a characteristic of changing resistance by 10 times when a magnetic field is applied.

In addition, a tunneling magnetoresistance (TMR) sensor can be utilized as the magnetoresistance sensor of the present invention. Tunneling magnetoresistance, using the above-described GMR theory, allows the middle layer to be replaced with an electrically insulator that is not a non-magnetic material. In theory, therefore, current cannot pass through the insulator. When the thickness is reduced to nano units, it refers to a technology and a system in which electrons are allowed to pass through by a tunneling effect, which is one of quantum mechanical effects, and refers to a sensor using the same.

MJT (Magnetic Tunneling Junction) sensor uses the same concept as TMR, and it can be applied to this. SDT (Spin Dependent Tunneling) also uses external spin up / down like GMR and TMR. It refers to using a method of measuring the resistance change value, all of which can be applied to the magnetoresistive sensor of the present invention.

Figure 2 is a block diagram of a rotation type bio diagnostic apparatus according to an embodiment of the present invention.

So the present invention is configured by combining the rotating unit 30 to the signal detecting unit 10 and the monitoring unit 20.

In this case, the rotating unit 30 mounts the sensing material 30 on the disk 31 of the rotation type to allow the signal sensing unit 10 to measure one or more sensing materials 30.

The signal detecting unit 10 includes a magnetoresistance (MR) sensor 12 measuring one or more sensing substances 40 mounted on the rotating unit 30, and sensing measured by the magnetoresistive (MR) sensor 12. The signal is transmitted to the monitoring unit 20.

The monitoring unit 20 receives and monitors the signal measured by the signal detecting unit 10.

Hereinafter, in describing a preferred embodiment of the present invention, the GMR among the various magnetoresistive (MR) sensors described above will be described as an example.

3 illustrates the principle of a magnetoresistive (MR) sensor in FIG. 2. In particular, among the variously applicable sensors as described above, it is a conceptual diagram showing a sensing principle by taking a giant magnetoresistance (GMR) sensor as an example. Specifically, the spin-valve type (Giant Magneto Resistance) device is shown.

Thus, when the nonmagnetic metal layer is sandwiched between two ferromagnetic metal layers, the magnetic force of the ferromagnetic metal layer of the first layer is fixed, and the magnetic force of the ferromagnetic material of the second layer is variably adjusted so that the first layer and the magnetic force are parallel. Only the electrons spin-oriented in a particular direction use the principle of passing through the conductor. That is, according to the alignment of the magnetization directions of the two ferromagnetic layers, a difference in electrical resistance induced inside the material or a potential difference occurs, which is recognized as a digital signal. The case where the interlayer material is a conductor is a GMR device.

4 is a conceptual diagram illustrating an example of coupling a signal detector and a monitor in FIG. 2. (As mentioned above, it is a matter of course that the various examples described above can also be applied to the magnetoresistive sensor.)

Thus, one or a plurality of biosensor cartridge GMR sensors 12 are installed in the bio-cartridge, which is the signal detection unit 10, to form the electrode pattern 15. The device of the GMR sensor 12 used at this time has a volume of 0.3 mm, 0.5 mm, and 1.0 mm. The saturation field is 15Oe, and the sensitivity is at least 3.0 to at most 4.2 (mV / V). -Oe) was used. The interface of the GMR sensor 12 itself was a 5W power supply using a Wheatstone bridge, and the sensing element was measured at about 5KΩ. In addition, the standard of the cartridge (Cartridge) using the GMR for the biosensor cartridge did not limit the minimum and maximum specifications, and the cartridge standard of the signal sensing unit 10 in relation to the number of GMR elements and the electrode pattern to be installed is To be varied.

In addition, the GMR sensor 12, which is a GMR for a biosensor, may be installed in the form of a bar chip or a package.

The GMR cartridge, which is the signal detection unit 10, is coupled to the GMR Bridge-Amp, which is the monitoring unit 20. The monitoring unit 20 installed a bridge amplifier, added a frequency filtering stage to remove noise at the power stage and noise at the output stage, and LMC7101 suitable for the bridge amplifier. An amplifier was used. In particular, the sensitivity of the signal was increased by installing three differential amplifiers, and a variable resistor was installed at the output terminal to amplify the sensitivity beyond the sensitivity of the sensor cartridge itself, which is the signal sensing unit 10 at the output terminal.

5 is a view illustrating a process of the signal detection unit in FIG. 2, FIG. 5A is a view showing a hole formed in a substrate, FIG. 5B is a view showing an electrode pattern deposited on a substrate, and FIG. 5C is a view showing wire bonding. 5D is a diagram illustrating epoxy molding.

Thus, one or more GMR sensors 12 were provided on the substrate 11 to form the electrode pattern 15.

In this case, the GMR sensor 12 may be formed directly on the substrate 11 or may be inserted into the substrate by making a groove in the substrate 11. That is, the control hole 13 and the test hole 14 may be formed in the substrate 11 in advance, and the signal sensing unit 10 may be configured by inserting the GMR sensor 12 into the holes 13 and 14.

The size of the substrate 11 using the GMR for the biosensor was set to a minimum of 3x1 Cm ² to a maximum of 5x1.5 Cm ² , but the length of the GMR sensor 12 and The thickness and the size of the substrate can be varied. The type of substrate used may be any material capable of fixing the GMR sensor 12 and forming the electrode pattern 15 such as glass or plastic.

As shown in FIG. 5C, an electrode formed by the GMR sensor 12 and the electrode pattern 15 of the substrate 11 is formed by wire-bonding 16. In addition, as shown in FIG. 5D, the electrode is protected by performing epoxy molding 17 using epoxy resin at the connection portion between the GMR sensor 12 and the electrode by wire bonding 16. This protects the electrode and the GMR sensor 12 and makes it possible to improve the durability against friction that may occur during measurement.

In addition, it is possible to install one or more GMR sensors 12 in the signal detection unit 10, which allows the test line and the control line of the signal detection unit that are being used to be measured immediately. Even if a new type signal detection unit is installed, the position and number of the GMR sensor 12 can be modified accordingly.

In addition, the signal detecting unit 10 applies a constant frequency to the GMR sensor 12 and outputs a detection signal to the monitoring unit 20.

FIG. 6 is a detailed conceptual view of the rotating part of FIG. 2, and FIG. 7 is a conceptual view illustrating a front part and a side part of the disc in FIG. 6.

The disk 31 is thus of rotational type and equipped with a sensing material 40. It is also fixed to the front and rear moving parts 33 by the rotating part 34.

The front and rear moving parts 33 are attached to the support part 32, and the other side is connected to and fixed to the disk 31, so that the disk 31 is moved forward and backward along the lower surface of the horizontal axis of the support part 32. do.

The vertical movement part 35 has one surface attached to the support part 32, and the other side fixes the signal detection part 10, and the signal detection part 10 is in the vertical direction along the side of the vertical axis of the support part 32. To be moved.

In addition, the rotating unit 34 connects the disk 31 to the front and rear moving unit 33, and causes the disk 31 to rotate so that the plurality of sensing materials 40 mounted on the disk 31 are detected by the signal sensing unit 10. To be detected.

Therefore, when detecting a plurality of sensing materials 40 mounted on the disk 31, the disk 31 may be rotated and sensed while the signal sensing unit 10 is fixed, or the disk 31 is fixed. In this state, the signal detecting unit 10 may be moved by moving in the vertical direction. In addition, the distance between the sensing material 40 and the signal sensing unit 10 may be adjusted by the front and rear moving units 33.

As described above, the GMR sensor 12 has a signal detecting unit 10 attached thereto and a portion of the disk 31 attached with the sensing material 40 to be sensed can be adjusted up, down, left and right. ) And the gap and position between the disk 31 can be freely adjusted. In addition, at least one sensing material (antigen or sample) 40 to be sensed on the surface of the disk 31 may be attached, and the attachable size may be freely adjusted within the size of the disk 31. The size of the disk 31 is not limited to the radius of 3.5cm, thickness 3mm. However, since the disk 31 rotates, it is used as a material that does not bend under the influence of the rotation, and the thickness of the disk 31 may also be determined in a range that does not bend in the number of revolutions to be used.

In addition, a linear motor may be used to rotate the disk 31, and a precision controller may be used to adjust the sensing position and the distance between the disk 31 and the GMR sensor 12.

FIG. 8 is a conceptual diagram illustrating a position of a GMR sensor and a sensing material (antigen or sample) in FIG. 6.

Thus, the position of the sensing material (antigen or sample) 40 and the GMR sensor 12 to be detected on the disk 31 may be arranged as shown in FIG. 8, and the up, down, left, and right sides of the measuring device are adjusted. Thus, the position of the GMR sensor 12 and the sensing material 40 may be optimized. The sensing material (antigen or sample) 40 attached on the disk 31 may be arranged directly on the surface of the disk 31, and a membrane used in a general rapid kit may be attached to the disk 31. It can be attached and measured. It is possible to freely adjust the measuring device up, down, left and right to attach various sizes and various materials to the disk.

9 is a waveform diagram of data measured using the rotating unit of FIG. 6. 9 is a measurement of the magnetic material placed on the disk as the actual measured data value, while rotating.

As shown in FIG. 9, when the positions of the sensing material 40 having the magnetic force and the GMR sensor 12 coincide with each other, the graph as shown by reference numeral 51 was repeatedly measured. The peak spacing in the graph can be influenced by the number of rotations. If multiple samples are to be measured, a reference peak can be determined and the multiple samples from the reference peak can be analyzed. In FIG. 9, reference numeral 52 denotes an output width desired by the monitoring unit 20.

Through this, the present invention can provide a bio signal detecting unit equipped with a magnetoresistive MR sensor for the biosensor, and the magnetoresistive MR sensor can be manufactured by installing one or more. In addition, by installing an electrode pattern in the signal sensing unit to be connected to the monitoring system, the magnetic biomaterial may be detected by amplifying and signal processing the signal output from the MR sensor. In addition, the magnetic signal can be detected in a non-contact way by rotating the disk. The material can be directly loaded on the disk (antibodies, DNA, specimen, etc.). Magnetic signals can be detected in a non-contact manner. The material to be detected also has a magnetic force and includes any material that can be attached to the disk. In addition, the present invention uses a method of detecting a magnetoresistive signal using a rotary (non-contact) system, the signal is detected by applying a constant frequency to the GMR sensor for measurement in the rotary (non-contact) system.

As described above, the present invention is to measure a plurality of bio materials through one measurement by mounting one or more sensing materials, which are magnetic materials coated with biomaterials (antigens or samples), on a disk of a rotating type.

Although the above has been described as being limited to the preferred embodiment of the present invention, the present invention is not limited thereto and various changes, modifications, and equivalents may be used. Therefore, the present invention can be applied by appropriately modifying the above embodiments, it will be obvious that such application also belongs to the scope of the present invention based on the technical idea described in the claims below.

1 is a conceptual diagram of a conventional giant magnetoresistive sensor and a sensing cell array using the same.

Figure 2 is a block diagram of a rotation type bio diagnostic apparatus according to an embodiment of the present invention.

3 is a conceptual diagram illustrating a sensing principle of a magnetoresistive (MR) sensor in FIG. 2.

4 is a conceptual diagram illustrating an example of coupling a signal detector and a monitor in FIG. 2.

5 is a view illustrating a process of the signal detection unit in FIG. 2, FIG. 5A is a view showing a hole formed in a substrate, FIG. 5B is a view showing an electrode pattern deposited on a substrate, and FIG. 5C is a view showing wire bonding. 5D is a diagram illustrating epoxy molding.

6 is a detailed conceptual view of a rotating part in FIG. 2.

FIG. 7 is a conceptual view illustrating a front part and a side part of a disc in FIG. 6.

FIG. 8 is a conceptual diagram illustrating a position of a magnetoresistive (MR) sensor and a sensing material (antigen or sample) in FIG. 6.

9 is a waveform diagram of data measured using the rotating unit of FIG. 6.

Explanation of symbols on the main parts of the drawings

10: signal detection unit 11: magnetoresistance (MR) sensor

12: control hole 13: test hole

14 electrode pattern 15 wire bonding

16: epoxy molding 20: monitoring unit

30: rotating part 31: disc

32: support part 33: front and rear moving part

34: rotating part 35: up and down moving part

40: sensing material

Claims (7)

A rotating portion for mounting the sensing material on the rotating disk; A signal detecting unit having a magnetoresistive (MR) sensor for measuring a sensing material mounted on the rotating unit; A monitoring unit for monitoring a signal measured by the signal detecting unit; Rotation type bio-diagnostic device, characterized in that configured to include. The method according to claim 1, The rotating part, A disk of rotation type and mounted with sensing material; A support; One side is attached to the support portion, the other side is connected to and fixed to the disk, and the front and rear moving parts for moving the disk in the front and rear directions; One side is attached to the support portion, the other side is fixed to the signal detection unit, the vertical movement unit for moving the signal detection unit in the vertical direction; Rotation type bio-diagnostic device, characterized in that configured to include. The method according to claim 2, The rotating part, A rotating part connecting the disk to the front and rear moving parts and allowing the disk to rotate; Rotation type bio-diagnostic device, characterized in that further comprises a. The method according to any one of claims 1 to 3, The signal detection unit, A substrate; A magnetoresistance (MR) sensor mounted on the substrate and outputting a detection signal when sensing the sensing material; An electrode pattern on which an electrode for transmitting a detection signal of the magnetoresistive (MR) sensor to a monitoring unit is formed; Wire bonding connecting the electrode of the electrode pattern to the magnetoresistive (MR) sensor; An epoxy molding protecting the connection portion of the wire bonding; Rotation type bio-diagnostic device, characterized in that configured to include. The method according to claim 4, The signal detection unit, A hole formed in the substrate such that the magnetoresistive (MR) sensor is mounted on the substrate; Rotation type bio-diagnostic device, characterized in that further comprises a. The method according to any one of claims 1 to 3, The signal detection unit, Rotation type bio-diagnostic device, characterized in that for outputting a detection signal by applying a constant frequency to the magnetoresistance (MR) sensor. The method according to claim 1, wherein the sensing material, Rotation type bio-diagnostic device, characterized in that any one of a membrane, an antigen or a diagnostic kit arranged or attached directly on the disk.

Images