KR20090126432A - Composition for treating or preventing cancer - Google Patents

Abstract

PURPOSE: A composition using Panax ginseng C.A. Meyer fruit extract is provided to ensure effective anticancer activity for treating cancer such as breast cancer and liver cancer. CONSTITUTION: A composition for preventing or treating cancer contains Panax ginseng C.A. Meyer fruit extract as an active ingredient. The Panax ginseng C.A. Meyer fruit extract is obtained by extracting pulp and peel of the fruit with solvent. The fruit extract is crude saponin fraction obtained by extracting and concentrating crude saponin. The fruit extract has 0.5-3.5 of PD(protopanaxadiol) saponin based on PT(protopanaxatriol) saponin. The extract contains mineral of potassium, calcium, iron, phosphorus, magnesium or zinc.

Description

Composition For Treating or Preventing Cancer

The present invention relates to a composition for treating or preventing cancer, which contains an extract of ginseng fruit containing saponin as an active ingredient.

Ginseng (Panax ginseng C.A. Meyer) is a plant belonging to the genus Ogapi ginseng and has been used in Korea, China, and Japan for more than 2,000 years, and has been used to prevent disease and extend life.

Known efficacy and effects of ginseng to the central nervous system, anticarcinogenic effect, anticancer activity, immune function regulation, antidiabetic effect, liver function anti-inflammatory effect, cardiovascular disorders improvement, anti-arteriosclerosis, blood pressure control action, menopausal Disability improvement, effects on osteoporosis, antistress and anti-fatigue, antioxidant activity and anti-aging effects (Korea Ginseng 'Ingredients and Efficacy', Korea Ginseng and Tobacco Research Institute, 56-112, 1996).

Ginsenoside (Ginseng Saponin), a representative physiologically active ingredient of Ginseng, is distributed evenly on the ground and underground parts of Ginseng, especially ginsenoside content depending on the parts such as ginseng root (root), Ginseng leaf and Ginseng fruit. The composition is also known to be different (Attele AS et al, Biochem Pharmacol, 58; 1685-1693, 1999).

Recently, as ginseng has been focused on the chemoprevention of the concept of inhibiting or delaying the multi-step cancer development process using non-toxic compounds, dietary ingredients or drugs, It is being studied as the main material. However, most of the ginsenoside components and ginseng polysaccharides of the extract or ginseng root using the ginseng root, the anti-cancer efficacy by the components of the ginseng fruit is the situation.

Ginseng fruit is considered more precious than ginseng and has been harvested for the purpose of seed acquisition. Generally, ginseng fruit is harvested only once in 4 years of growing season. Fruit extract of American ginseng (Panax qllinqllcfoliwn L.) has anticancer efficacy against colon cancer (J Agric Food Chem. 54 (26): 9936-42) and has the effect of inhibiting the growth of human breast cancer cell line (MCF-7) Although it has been found that this is a different species from the Korean ginseng (Panax ginseng CA Meyer) used in the present invention is a natural product that is completely different in its composition.

In this regard, cancer is a malignant tumor that is composed of undifferentiated cells which are infinitely multiply and ignore proliferative conditions in the tissues, unlike normal cells, which are capable of regular and modest proliferation and inhibition. Say.

Despite the rapid development of modern medicine, the world's cancer incidence is increasing by more than 5% every year due to deterioration of the environment and the increase of elderly population. According to statistics from the 2002 National Cancer Center, men develop cancer in the order of stomach cancer, liver cancer, lung cancer and colon cancer, and the mortality rates from cancer are liver cancer, lung cancer and stomach cancer. This ranking difference between incidence and mortality is due to the characteristics of liver cancer, which is very difficult to treat once developed. On the other hand, women's cancer incidence was 16.1% of breast cancers, which was the second year of cancer in 2001, exceeding stomach cancer (15.3%). This is an increase of 66% compared to 1995, the highest rate of increase among cancers.

To date, surgical treatment, biotherapy and radiation therapy have been used for the treatment of cancer. However, recognizing that these classical therapies are not effective and fundamental methods for lowering the risk of cancer, efforts are being made to improve the biological defense ability by enhancing immune function and to develop chemoprevention agents.

Such chemical cancer prevention is intended to inhibit, delay or reverse the cancer cellization of normal cells using relatively safe chemicals without toxicity or side effects. Chemical cancer preventive agents should be extremely mild, even if they are primarily non-toxic or absent. Thus, naturally derived ingredients that have long been proven safe have been studied as promising chemical cancer preventive candidates, and most of the substances known as chemical cancer preventive agents are actually derived from plants.

Nevertheless, Panax ginseng C.A. containing saponins. No anti-cancer composition comprising Meyer's ginseng fruit extract as an active ingredient has been reported, and thus, as described below, the present invention has a significant cancer treatment effect, in particular, a significant increase in the incidence of breast cancer and liver cancer. Provide compositions that work.

Accordingly, an object of the present invention is to solve the problems of the prior art as described above and the technical problems that have been requested from the past.

Specifically, an object of the present invention is Panax ginseng C.A. Meyer ginseng fruit through the composition comprising the extract as an active ingredient, to provide a composition for treating or preventing cancer exhibiting excellent anticancer efficacy.

Cancer treatment or prevention composition according to the present invention for achieving this object is composed of a fruit extract of ginseng (Panax ginseng C.A. Meyer) containing saponin as an active ingredient.

The inventors of the present application, as shown through the following examples, according to the present invention Panax ginseng C.A. When applying a composition containing Meyer's ginseng fruit extract as an active ingredient to cancer cell lines, it has been found that can effectively inhibit the proliferation of cancer cells, through which the composition of the present invention is very effective in the treatment or prevention of cancer Confirmed.

The extract may be included in an amount of 0.01 to 100% by weight based on the total composition, when the content of the extract is included in an amount of 100% by weight of the total composition, the composition is an active ingredient exhibiting a therapeutic or prophylactic effect of cancer It may be composed only of the extract.

In one preferred embodiment, the extract may be prepared by drying the pulp and skin of the ginseng (Panax ginseng C.A. Meyer) fruit removed by separating the seeds, for example, after drying with sunlight or hot air drying.

As described above, the process of removing the seeds and drying or hot-air drying the flesh and skin of the ginseng (Panax ginseng CA Meyer) fruit is a process of pretreatment of the ginseng (Panax ginseng CA Meyer) fruit, ginseng prepared through Panax ginseng CA Meyer) fruit dried can be extracted by treating with a solvent, preferably water or ethanol, and concentrated under reduced pressure to prepare a fruit extract of Panax ginseng CA Meyer.

In another preferred embodiment, the composition may include a probeponin fraction prepared by subjecting the extract to a solvent prepared by the pre-treatment and extraction step again, extracting and concentrating the probeponin of the fruit of Panax ginseng CA Meyer have.

Specifically, by treating a solvent, preferably ether, to the extract prepared through the pre-treatment and extraction step, the fat-soluble component can be removed, and the irradiated with a butanol (BuOH) and irradiated with a siphonponin fraction can be prepared. As a result, the inventors of the present application confirmed that the composition comprising the irradiated ginseng (Panax ginseng C.A. Meyer) fruit of the irradiated saponin fraction as an active ingredient exhibits particularly excellent cancer cell growth inhibitory effect, that is, anticancer efficacy.

In one preferred embodiment, the total amount of saponin contained in the extract may be 20 to 70% by weight, preferably 25 to 65% by weight based on the total extract, the extract is PT (Protopanaxatriol) -based saponin The ratio of Protopanaxadiol (PD) -based saponin to (ginsenoside) may be 0.5 to 3.5, preferably 0.6 to 3.0.

In addition, the extract is one or more rich minerals selected from the group consisting of potassium, calcium, iron, phosphorus, magnesium and zinc, and / or vitamin B1, vitamin B2, vitamin B6, vitamin C, vitamin E, vitamin K, niacin (vitamin B3) and folic acid (vitamin B9).

Compared to ginseng root, saponin content is very high, and the composition of PT (Protopanaxatriol) saponins "saponins Rb1, Rb2, Rc and Rd" and PD (Protopanaxadiol) saponins "saponin Re, Rg1 and Rg2" are completely different. It is a unique composition of the composition including the fruit extract of Panax ginseng CA Meyer, which has abundant minerals and vitamins, as well as its fruit characteristics, which is remarkable in the composition and composition of the composition using the known ginseng root extract as an active ingredient. It is clear that there is a difference.

The composition according to the present invention is effective for treating or preventing cancer, and the cancer may be breast cancer and liver cancer. In one preferred embodiment, the composition can exert an effect of inhibiting proliferation, in particular of breast cancer and liver cancer cell lines.

The composition according to the present invention may be a food composition and a pharmaceutical composition containing a ginseng (Panax ginseng C. A. Meyer) fruit extract as an active ingredient, the food composition may be a health functional food composition.

The pharmaceutical composition according to the present invention may be administered orally in the form of solid, semisolid or liquid by adding a commercially available inorganic or organic carrier, or parenteral, rectal, topical, transdermal, intravenous, intramuscular, intraperitoneal, subcutaneous. And the like, especially oral administration is preferred.

The preparation for oral administration includes tablets, pills, granules, soft and hard capsules, powders, fine granules, powders, emulsions, syrups, pellets and beverages. Etc. can be mentioned. In addition, preparations for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like.

In order to formulate the active ingredient of the present invention, it can be easily formulated according to the conventional method, and surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffers, suspensions, and other commonly used auxiliaries can be suitably used.

In addition, the dosage of the active ingredient will vary depending on the age, sex and weight of the subject to be treated, the specific disease or pathology to be treated, the severity of the disease or pathology, the route of administration and the judgment of the prescriber. Dosage determination based on these factors is within the level of those skilled in the art, and generally the dosage may range from 0.001 mg / kg / day to approximately 2000 mg / kg / day.

Hereinafter, the present invention will be described in more detail with reference to Examples, but the following Examples are provided to illustrate the present invention, and the scope of the present invention is not limited thereto.

 Example 1 Preparation of Panax ginseng C.A. Meyer Fruit Extract

1) Ginseng (Panax ginseng CA Meyer) fruit pretreatment: Ginseng (Panax ginseng CA Meyer) fruit is harvested, the seeds are separated and removed, and then the flesh and skin of Panax ginseng CA Meyer fruit are dried by sunlight or hot air drying. (Panax ginseng CA Meyer) fruit dried raw material was prepared.

2) Preparation of ginseng (Panax ginseng CA Meyer) fruit extract: 1L of ginseng (Panax ginseng CA Meyer) fruit extract was added to 3 kg of water and reflux extracted, filtered and concentrated under reduced pressure at 40 ~ 45 ℃ ginseng (Panax ginseng CA Meyer) fruit extract 300 g of fruit extract was obtained.

Example 2 Preparation of Irradiated Gonponin Fraction from Panax ginseng C.A. Meyer Fruit Extract

Ginseng (Panax ginseng C.A. Meyer) fruits and extracts were prepared, and the extracts were treated with ether to remove fat-soluble components.

Comparative Example 1 Preparation of Ginseng Root Extract

Except for using ginseng root (root) instead of ginseng (Panax ginseng C.A. Meyer) fruit in Example 1, was prepared in the same manner as in Example 1.

Experimental Example 1 Component Comparison of Panax ginseng C.A. Meyer Fruit Extract

Analysis of Ginsenoside (Ginseng Saponin) Components of Panax ginseng C.A.Meyer Fruit and Ginseng Root

Ginsenoside component analysis was performed by HPLC using Examples 1 and 2 and Comparative Example 1, and the results are shown in Table 1.

The ginseng (Panax ginseng CA Meyer) fruit extract prepared in Example 1 and the irradiated saponin fraction prepared in Example 2 were each about 2 times higher than the ginseng root extract prepared in Comparative Example 1 in the total saponin content, It contained four times the content of ginsenosides, and ginsenosides were PD (Protopanaxadiol: Ginsenosides Rb1, Rb2, Rc and Rd) -based ginsenosides and PT (Protopanaxatriol: Ginsenosides Re, Rg1 and Rg2) -based ginsenosides. When divided by the ratio of, the values of Comparative Example 1, Example 1, and Example 2 were 3.54, 1.78, 0.76, respectively, in the composition of Panax ginseng CA Meyer fruit and ginseng root showed distinct differences and characteristics. It was.

Mineral Analysis of Panax ginseng C.A. Meyer Fruit Extract

In order to distinguish the ginseng (Panax ginseng CA Meyer) fruit extract prepared in Example 1 has the characteristics of 'fruit' different from ginseng, mineral components including vitamins were analyzed, and the results are shown in Table 2 below. .

As described above, the ginseng (Panax ginseng CA Meyer) fruit composition used in the present invention contains more ginseng saponin than ginseng root, and the nature of the saponin composition is the opposite, and also unlike the ginseng root vitamin And it was confirmed that the rich content of 16 kinds of minerals, based on the following experiments on the anticancer efficacy of the fruit of Panax ginseng CA Meyer.

Experimental Example 2 Evaluation of Anticancer Effects of Panax ginseng C.A. Meyer Fruit Extract

One. Growth Inhibitory Effects of Panax ginseng C.A. Meyer Fruit Extracts on Breast Cancer Cell Lines

Cell culture and material processing

In order to evaluate the cancer cell growth efficacy of Panax ginseng CA Meyer fruit extract, cell survival rate was quantitatively measured using MTT assay. For MTT assay, breast cancer (MDA-MB-231) or liver cancer (HepG2) cells were placed in a 48-well dish at a density of 1 × 10 ⁴ / well, followed by 10% FBS, 100 IU / ml penicillin and 100 g / ml streptomycin. DMEM (Dulbeccos Modification of Eagles Medium, Sigma Co.) comprising a culture medium was continuously supplied with a mixed gas of air (95%) and CO2 (5%) in a 37 ℃ incubator. After 24 hours after transplanting the cells into the culture vessel, the culture medium was replaced with FBS free DMEM, and the sample to measure the activity was treated to the cultured cells at final concentrations of 100 and 250 μg / ml, and nothing was treated as a control. After the test sample was added and incubated for 48 hours, MTT solution (final concentration 1 mg / ml) was added and incubated for 2 hours. Thereafter, the culture medium was removed and Dimethyl sulfoxide (DMSO) was added to measure the optical density at 540 nm with a microplate reader. Breast cancer and liver cancer cell proliferation inhibitory efficacy results are shown in Table 3 and Figure 1, liver cancer cell proliferation inhibitory efficacy results are shown in Table 4 and FIG.

Cell viability (% of control) = (absorbance of material treated group / absorbance of control group) X 100

Through Table 3 and Figure 1, it can be confirmed that ginseng (Panax ginseng CA Meyer) fruit extract inhibits the proliferation of breast cancer cell line more than ginseng root extract, ginseng (Panax ginseng CA Meyer) fruit extract irradiated It can be seen that the more effectively inhibit the growth of breast cancer cell line.

In addition, while the ginseng root extract hardly inhibits the proliferation of liver cancer cell lines, the ginseng (Panax ginseng CA Meyer) fruit extract was treated with a final concentration of 250 μg / ml and the ginseng ( Panax ginseng CA Meyer) When the final concentration of the irradiated ponponin fraction of the fruit extract is 100, 250 μg / ml, it can be confirmed that the proliferation of liver cancer cell line is effectively inhibited.

This suggests that ultimately, when ginseng (Panax ginseng C.A. Meyer) fruit extract according to the present invention contains anti-cancer efficacy by effectively inhibiting the proliferation of breast cancer, liver cancer cells.

Example 3 Preparation of Pill

Ginseng (Panax ginseng C.A. Meyer) fruit extract prepared in Example 1, 30% by weight of corn starch, 20% by weight glycerin and 20% by weight of sorbitol powder were mixed, and the ring was prepared using a stirrer. The final weight of the contents was 3.5 g.

Example 4 Preparation of Tablet

30% by weight of Panax ginseng CA Meyer fruit extract prepared by Example 1, 20.5% by weight lactose, 20% by weight dextrin, 20% by weight maltitol powder and 7% by weight xylitol powder were mixed, using a fluid bed dryer After granulation, 2.5% by weight of sugar ester was added to a tablet press. The final weight of the contents was 2 g.

Example 5 Preparation of Granules

30% by weight of Panax ginseng C.A. Meyer fruit extract prepared by Example 1, 5% by weight of xylitol and 65% by weight of isomalt were mixed and molded into granules using a fluidized bed granulator and then filled into fabrics. The final weight of the contents was 2 g.

As described above, the composition according to the present invention, by containing a ginseng (Panax ginseng CA Meyer) fruit extract having an ingredient and composition different from the ginseng root in the ground portion of ginseng as an active ingredient, excellent anticancer against cancer, especially breast and liver cancer Can be effective.

Those skilled in the art to which the present invention pertains will be able to perform various applications and modifications within the scope of the present invention based on the above contents.

1 is a graph showing the results of evaluating breast cancer cell inhibition efficacy of the group treated with the composition according to the present invention;

Figure 2 is a graph showing the results of evaluating the effect of inhibiting liver cancer cells of the group treated with the composition according to the present invention.

Claims (9)

Cancer ginseng (Panax ginseng C.A. Meyer) containing saponin composition for the treatment or prevention of cancer comprising the extract as an active ingredient. The method of claim 1, wherein the extract is a composition for treating or preventing cancer, characterized in that the extract is manufactured by drying the flesh and skin of the ginseng fruit with a solvent. According to claim 1, wherein the extract is a ginseng (Panax ginseng C.A. Meyer) composition for the treatment or prophylaxis of cancer characterized in that the fraction of the saponponin prepared by extracting and concentrating the saponin of the fruit. According to any one of claims 1 to 3, wherein the saponin is a composition for treating or preventing cancer, characterized in that it comprises 20 to 70% by weight based on the total dry weight of the extract. According to any one of claims 1 to 3, wherein the extract is PD (Protopanaxatriol) of the PD (Protopanaxadiol) -based saponin ratio of PT to Protopanaxatriol-based saponin is a composition for the treatment or prevention of cancer, characterized in that 0.5 to 3.5. The method of claim 1, wherein the extract comprises one or more minerals selected from the group consisting of potassium, calcium, iron, phosphorus, magnesium and zinc. The method according to any one of claims 1 to 3, wherein the extract is in the group consisting of vitamin B1, vitamin B2, vitamin B6, vitamin C, vitamin E, vitamin K, niacin (vitamin B3) and folic acid (vitamin B9) A composition for treating or preventing cancer, characterized in that it comprises one or more selected vitimins. The method of claim 1, wherein the extract is a cancer treatment or prevention composition, characterized in that it comprises 0.01 to 100% by weight based on the entire composition. According to claim 1, wherein the cancer is cancer or liver cancer, characterized in that the composition for treating or preventing cancer.

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