KR20090088757A

KR20090088757A - Data processing, analysis method of gene expression data to identify endogenous reference genes

Info

Publication number: KR20090088757A
Application number: KR1020080014214A
Authority: KR
Inventors: 신영기; 권미정; 오은설; 인용호; 고상석
Original assignee: 재단법인서울대학교산학협력재단
Priority date: 2008-02-15
Filing date: 2008-02-15
Publication date: 2009-08-20

Abstract

A method for treating and analyzing gene expression data is provided to totally analyze data in data base and identify standard expression gene. A method for selecting candidate standard expression gene comprises: a step of digitalizing the expression amount of gene in EST, SAGE and microarray data set; a step of selecting a gene which is expressed in different tissues with zero rate and expression amount of gene; In the digitalization of gene expression amount, the math equation 1 and 2 is used. The math equation 1 is EST gene expression amount = (the number of EST of specific gene in specific library/the number of total EST in specific library) X 1000000, and math equation 2 is SAGE gene expression amount = (the number of tag of specific gene in specific library / the number of total tag in specific library) X 1000000.

Description

Data processing, analysis method of gene expression data to identify endogenous reference genes}

본 발명은 표준 발현 유전자(Endogenous Reference Genes; ERG)를 발굴하기 위한 유전자 발현 데이터 처리, 분석 방법 및 상기 방법에 의해 수득된 표준 발현 유전자를 특이적으로 증폭할 수 있는 프라이머쌍 및/또는 프로브를 포함하는 유전자 발현율 정량용 조성물에 관한 것으로, 더욱 상세하게는 EST, SAGE 및 마이크로어레이 데이터를 대상으로 제로비율 및 변동계수를 이용하여 선별하는 데이터 처리, 분석 방법과 상기 방법에 의해 수득된 표준 발현 유전자를 특이적으로 증폭할 수 있는 프라이머쌍 및/또는 프로브를 포함하는 유전자 발현율 정량용 조성물에 관한 것이다.The present invention includes gene expression data processing, analysis methods for discovering Endogenous Reference Genes (ERGs), and primer pairs and / or probes capable of specifically amplifying the standard expression genes obtained by the above methods. The present invention relates to a composition for quantifying a gene expression rate, and more particularly, to a data processing, an analysis method, and a standard expression gene obtained by the method for selecting EST, SAGE, and microarray data using a zero ratio and a coefficient of variation. The present invention relates to a composition for quantifying gene expression, including a primer pair and / or a probe which can be specifically amplified.

각각의 세포에는 5만 내지 10만 개 정도의 유전자가 있지만, 세포는 선택적으로 유전자를 사용한다. 그 중 상당수는 세포 자신의 생명유지활동이나 일상적인 활동을 하기 위한 유전자이다. 이러한 유전자를 유지 유전자(housekeeping gene; 이하 HKG)라고 부른다. 또한, 내인성 표준 발현 유전자는, 특정 유전자의 기능을 밝히거나, 특정 기능의 유전자를 탐색하거나, 특정 조건의 생물체의 발현 양상 작성 및 그 외의 다양한 분자생물학적 목적에 의해, 특정 혹은 다수의 유전자 발현량의 비교를 위해 개발된 전령 RNA(messenger RNA; 이하 mRNA)의 정량분석을 이용하는 다양한 유전자발현 측정법에서, 측정하고자 하는 표적 유전자의 mRNA의 발현량의 상대적 정량화를 위한 표준화(normalization)에 활용되고 있는 유지 유전자를 의미한다.Each cell has between 50,000 and 100,000 genes, but cells optionally use genes. Many of them are genes for the cell's own life-sustaining or daily activities. This gene is called a housekeeping gene (HKG). In addition, endogenous standard expression genes may be used to identify specific or multiple gene expression amounts by identifying functions of specific genes, searching for genes with specific functions, or preparing expression patterns of organisms under specific conditions and various other molecular biological purposes. In various gene expression assays using quantitative analysis of messenger RNA (hereinafter, referred to as mRNA) developed for comparison, maintenance genes utilized for normalization for the relative quantification of mRNA expression levels of target genes to be measured. Means.

내인성 표준 발현 유전자는 다른 시료 간의 비교적 정확한 유전자 발현 비교를 위해 mRNA 발현량의 표준화에 가장 보편적으로 사용돼왔다(Vandesompele J et al., Genome Biol 3(7), p. RESEARCH0034, 2002). 즉, 전통적인 역전사중합효소연쇄반응법(reverse transcriptase polymerase chain reaction: 이하 RT-PCR)에서 최근 개발된 정량적 실시간 중합효소연쇄반응법(quantitative real time PCR: 이하 qRT-PCR), 유전자발현계열분석(serial analysis of gene expression: 이하 SAGE) 및 마이크로어레이(Microarray)에 이르는 유전자 발현 분석법들에 내인성 표준 발현 유전자를 보편적으로 사용하고 있다. 내인성 표준 발현 유전자로써 통상적으로 활용되는 글리세르알데히드삼인산탈수소효소(glyceraldehyde-3-phosphate dehydrogenase: 이하, GAPDH) 및 β-액틴(β-actin: 이하 ACTB)의 유전자들은 상기 유전자들이 세포 및 조직종류에 상관없이 샘플 간에 일정한 수준으로 발현되며 실험적 처리에 의해 변하지 않을 것이라는 가정하에 적절한 검증 없이 사용돼 오고 있다.Endogenous standard expression genes have been most commonly used to standardize mRNA expression levels for comparatively accurate gene expression comparisons between different samples (Vandesompele J et al., Genome Biol 3 (7), p. RESEARCH0034, 2002). In other words, the recently developed quantitative real time PCR (qRT-PCR), gene expression sequence analysis (serial) in the traditional reverse transcriptase polymerase chain reaction (RT-PCR) endogenous standard expression genes are commonly used in gene expression assays ranging from SAGE) to Microarray. The genes of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin (ACTB) commonly used as endogenous standard expression genes can be used for cell and tissue types. It has been used without proper validation, assuming that it is expressed at a constant level between samples and will not change by experimental processing.

그러나 전통적인 내인성 표준 발현 유전자의 발현이 조직 및 세포종류에 따라 다르며 시료의 처리, 발생학적 단계(developmental stage) 및 병리학적 상태(pathological states)를 포함한 실험 조건들에 의해 조절될 수 있다는 사실은 이미 잘 알려져 있다(Bereta J and Bereta M, Biochem Biophys Res Commun 217(1)363-369, 1995; Tricarico C et al., Anal Biochem 309(2):293-300, 2002; Thellin O et al., J Biotechnol 75(2-3):291-295, 1999; Rubie C et al., Mol Cell Probes 19(2):101-109, 2005; Schmittgen TD and Zakrajsek BA, J Biochem Biophys Methods 46(1-2):69-81, 2000; Zhong H and JSimons W, Biochem Biophys Res Commun 259(3):523-526, 1999; Selvey S et al., Mol Cell Probes 15(5):307-311, 2001; Wu YY and LRees J, Acta Derm Venereol 80(1):2-3, 2000; Lee PD et al., Genome Res 12(2):292-297, 2002; Hamalainen HK et al., Anal Biochem 299(1):63-70, 2001). 상대 정량(relative quantification)시 부적절한 내인성 표준 발현 유전자를 사용함으로써 잘못된 발현 분석이 이루어질 수 있으며, 이러한 우려는 이미 여러 연구자가 제기하고 있다(Tricarico C et al., Anal Biochem 309(2):293-300 2002; Dheda K et al., Anal Biochem 344(1):141-143, 2005; de Kok JB et al., Lab Invest 85(1):154-159, 2005; Brunner AM et al., BMC Plant Biol 4:14, 2004). 비록 노던 블롯(northern blot)이나 전통적인 RT-PCR 같은 정성적인 성향을 띄는 mRNA량의 비교분석에서는, 정확한 표준화가 필요하지 않을 수 있지만, 높은 민감도(sensitivity)와 정확성(accuracy)의 결과를 나타내는 유전자 발현 측정법인 qRT-PCR의 경우에서는, 특히 적절한 내인성 표준 발현 유전자의 선 택은 매우 중요하다(Huggett J et al., Genes Immun 6(4):279-284, 2005).However, it is well established that the expression of traditional endogenous standard expression genes varies by tissue and cell type and can be controlled by experimental conditions, including sample processing, developmental stages, and pathological states. Bereta J and Bereta M, Biochem Biophys Res Commun 217 (1) 363-369, 1995; Tricarico C et al., Anal Biochem 309 (2): 293-300, 2002; Thellin O et al., J Biotechnol 75 (2-3): 291-295, 1999; Rubie C et al., Mol Cell Probes 19 (2): 101-109, 2005; Schmittgen TD and Zakrajsek BA, J Biochem Biophys Methods 46 (1-2): 69-81, 2000; Zhong H and JSimons W, Biochem Biophys Res Commun 259 (3): 523-526, 1999; Selvey S et al., Mol Cell Probes 15 (5): 307-311, 2001; Wu YY and LRees J, Acta Derm Venereol 80 (1): 2-3, 2000; Lee PD et al., Genome Res 12 (2): 292-297, 2002; Hamalainen HK et al., Anal Biochem 299 (1): 63 -70, 2001). Inadequate expression analysis can be achieved by using inappropriate endogenous standard expression genes in relative quantification, and this concern has already been raised by several researchers (Tricarico C et al., Anal Biochem 309 (2): 293-300). 2002; Dheda K et al., Anal Biochem 344 (1): 141-143, 2005; de Kok JB et al., Lab Invest 85 (1): 154-159, 2005; Brunner AM et al., BMC Plant Biol 4:14, 2004). Although comparative analysis of qualitatively quantitative mRNA levels, such as Northern blots or traditional RT-PCR, may not require precise standardization, gene expression may result in high sensitivity and accuracy. In the case of the qRT-PCR assay, the selection of appropriate endogenous standard expression genes is particularly important (Huggett J et al., Genes Immun 6 (4): 279-284, 2005).

전통적인 내인성 표준 발현 유전자들의 적절한 검증 및 더 적합한 내인성 표준 발현 유전자 탐색의 중요성이 인식되면서 보편적으로 사용되는 내인성 표준 발현 유전자 중에서 특정 실험 조건에서 가장 적절한 유전자를 선택하거나, mRNA 정량을 위해 일반적으로 쓰이는 전통적인 내인성 표준 발현 유전자보다 우월한 새로운 유전자들을 찾기 위한 많은 연구들이 수행돼 오고 있다. 그러나 대부분의 기존 연구들은 보편적으로 사용되는 내인성 표준 발현 유전자 중 특정 실험 시스템이나 제한된 조직들에서 가장 안정적인 발현을 보이는 유전자들을 선택하는데 집중돼 왔다(Goidin D et al., Anal Biochem 295(1):17-21, 2001; Haller F et al., Anal Biochem 335(1):1-9, 2004; Ohl F et al., J Mol Med 83(12):1014-1024, 2005; Radonic A et al., Biochem Biophys Res Commun 313(4):856-862, 2004). 현재 qRT-PCR 결과를 사용하여 다수의 내인성 표준 발현 유전자 중 가장 적합한 유전자들을 찾는 몇몇 프로그램들이 존재한다(Vandesompele J et al., Genome Biol 3(7), p. RESEARCH0034, 2002; Pfaffl MW et al., Biotechnol Lett 26(6):509-15, 2004; Andersen CL et al., Cancer Res 64(15):5245-5250, 2004).With the recognition of the importance of proper verification of traditional endogenous standard expression genes and the search for more suitable endogenous standard expression genes, the selection of the most appropriate genes for specific experimental conditions among the commonly used endogenous standard expression genes, or the traditional endogenous genes commonly used for mRNA quantification Many studies have been conducted to find new genes superior to standard expression genes. However, most existing studies have focused on the selection of the most commonly used endogenous standard expression genes with the most stable expression in specific experimental systems or restricted tissues (Goidin D et al., Anal Biochem 295 (1): 17 -21, 2001; Haller F et al., Anal Biochem 335 (1): 1-9, 2004; Ohl F et al., J Mol Med 83 (12): 1014-1024, 2005; Radonic A et al., Biochem Biophys Res Commun 313 (4): 856-862, 2004). There are currently several programs using the qRT-PCR results to find the most suitable genes among a number of endogenous standard expression genes (Vandesompele J et al., Genome Biol 3 (7), p. RESEARCH0034, 2002; Pfaffl MW et al. , Biotechnol Lett 26 (6): 509-15, 2004; Andersen CL et al., Cancer Res 64 (15): 5245-5250, 2004).

더불어, 새로운 내인성 표준 발현 유전자의 탐색은 주로 마이크로어레이 데이터에 근거해 왔다(Hamalainen HK et al., Anal Biochem 299(1):63-70, 2001; Hoerndli FJ et al., Anal Biochem 335(1):30-41, 2004; Czechowski T et al., Plant Physiol 139(1):5-17, 2005; Jin P et al., BMC Genomics, 5(1):55, 2004; Kobayashi MS et al., J Neurosci Res 76(4):512-518, 2004; Shulzhenko N et al., Biochem Biophys Res Commun 337(1):306-12, 2005). 그러나 이미 알려진 대로 마이크로어레이는 그 방법 상에서 프로브(probes)와 의도하지 않은 전사체들(transcripts)과의 부정확한 교차 혼성화(cross hybridization), 다른 프로브 세트(probe set)와 혼성화 효율(hybridization efficiencies) 및 프로브와 전사체의 결합 시 부정확성(transcript annotation)과 같은 몇 가지 문제점 및 제한(오류)들을 가지고 있다(Haverty PM et al., Bioinformatics 20(18):3431-3441, 2004; van Ruissen F et al., BMC Genomics 6:91, 2005). 또한 발현 유전자(expressed sequence tag: 이하 EST) 및 SAGE가 시료(cDNA libraries) 전체의 전사체들(transcripts)의 발현 양상을 볼 수 있는 것과 달리 마이크로어레이는 칩(chip)상에 있는 유전자들의 발현만 측정할 수 있는 한계가 있다(van Ruissen F et al., BMC Genomics 6:91, 2005). 그래서 여러 방법으로 생성된 유전자 발현데이터들을 같이 사용하는 것이 한 방법에 의해 생성된 데이터의 제한 점을 보완해 줄 수 있을 것으로 기대된다. 예를 들어 SAGE 방법은 EST보다 적은 량 존재하는 전사체(low abundance transcripts)를 측정하는데 더 민감한 방법이다(Sun M et al., BMC Genomics 5(1):1-4, 2004).In addition, the search for new endogenous standard expression genes has been based primarily on microarray data (Hamalainen HK et al., Anal Biochem 299 (1): 63-70, 2001; Hoerndli FJ et al., Anal Biochem 335 (1)). : 30-41, 2004; Czechowski T et al., Plant Physiol 139 (1): 5-17, 2005; Jin P et al., BMC Genomics, 5 (1): 55, 2004; Kobayashi MS et al., J Neurosci Res 76 (4): 512-518, 2004; Shulzhenko N et al., Biochem Biophys Res Commun 337 (1): 306-12, 2005). However, as is already known, microarrays inaccurate cross hybridization of probes with unintended transcripts, hybridization efficiencies with other probe sets, and There are some problems and limitations (errors) such as transcript annotation when combining probes and transcripts (Haverty PM et al., Bioinformatics 20 (18): 3431-3441, 2004; van Ruissen F et al. , BMC Genomics 6:91, 2005). Also, unlike expressed sequence tags (ESTs) and SAGEs, the expression of transcripts throughout cDNA libraries can be seen in microarrays only. There is a limit to measurable (van Ruissen F et al., BMC Genomics 6:91, 2005). Therefore, the use of gene expression data generated by various methods is expected to complement the limitations of the data generated by one method. For example, the SAGE method is more sensitive to measuring low abundance transcripts that are present in less than EST (Sun M et al., BMC Genomics 5 (1): 1-4, 2004).

이상적인 내인성 표준 발현 유전자가 존재하지 않을 수도 있지만 여러 대규모 유전자 발현 데이터를 통해 대부분의 실험조건에 적용될 수 있는 전통적인 내인성 표준 발현 유전자보다 더 이상적인 보편적으로 사용 가능한 내인성 표준 발현 유전자를 찾는 것은 가능하다.Although ideal endogenous standard expression genes may not be present, it is possible to find a universally available endogenous standard expression gene that is more ideal than traditional endogenous standard expression genes that can be applied to most experimental conditions through several large gene expression data.

이에, 본 발명자들은 공개된 EST 및 SAGE 유전자 발현량 데이터와 함께, 마이크로어레이 데이터를 이용하여 더 많은 시료에서 더 안정된 발현을 보임으로써 특정 조직이나 연구에 국한되지 않고 보편적으로 유전자 발현 표준화에 유용하게 사용될 수 있는 기존 유전자들보다 더 나은 새로운 내인성 표준 발현 유전자를 발굴하기 위한 유전자 발현 데이터의 처리방법과 상기의 방법에 의해 발굴된 내인성 표준 발현 유전자를 밝힘으로써 본 발명을 완성하였다.Accordingly, the present inventors, along with the published EST and SAGE gene expression data, show more stable expression in more samples using microarray data, which is not limited to specific tissues or studies, but is generally useful for standardizing gene expression. The present invention has been completed by revealing a method of processing gene expression data for discovering new endogenous standard expression genes better than existing genes and endogenous standard expression genes discovered by the above method.

본 발명의 목적은, 기존의 표준 발현 유전자보다 더 안정된 발현을 보임으로써, 유전자 발현 표준화에 유용하게 사용될 수 있는 새로운 내인성 표준 발현 유전자를 발굴하기 위해 통계적 개념을 도입한 상이한 유전자 발현 데이터 통합적 처리, 분석 방법과 상기 내인성 표준 발현 유전자를 증폭시킬 수 있는 프라이머쌍 및/또는 프로브를 포함하는 유전자 발현율 정량용 조성물을 제공하는 것이다.An object of the present invention is to integrate expression and processing of different gene expression data, which introduces a statistical concept to discover new endogenous standard expression genes that can be useful for standardizing gene expression by showing more stable expression than existing standard expression genes. It is to provide a method for quantifying gene expression rate comprising a method and a primer pair and / or probe capable of amplifying the endogenous standard expression gene.

상기 목적을 달성하기 위하여, 본 발명은 In order to achieve the above object, the present invention

1) EST, SAGE 및 마이크로어레이 데이터 세트에서 유전자의 발현량을 수치화하는 단계; 및,1) quantifying the expression levels of genes in the EST, SAGE and microarray data sets; And,

2) 단계 1)의 유전자 발현량과 제로비율을 이용하여 여러 조직에서 항상 발현되는 유전자를 선별하는 단계를 포함하는 후보 표준 발현 유전자(Endogenous Reference Genes; ERG)의 선별방법을 제공한다.2) Provides a method for screening candidate Endogenous Reference Genes (ERGs) including selecting genes that are always expressed in various tissues using the gene expression amount and zero ratio of step 1).

또한, 상기 방법에 의하여 선별된 후보 표준 발현 유전자를 검출할 수 있는 검출 시약을 포함하는 상기 후보 표준 발현 유전자의 검출용 조성물을 제공한다.The present invention also provides a composition for detecting the candidate standard expression gene, comprising a detection reagent capable of detecting the candidate standard expression gene selected by the method.

또한, 본 발명은 1) 정량 대상 유전자를 증폭할 수 있는 프라이머쌍 및/또는 프로브를 이용하여 상기 정량 대상 유전자를 실시간 PCR로 증폭하고, 상기 조성물을 이용하여 시료에 포함되어 있는 후보 표준 발현 유전자를 실시간 PCR로 증폭하 는 단계; 및In addition, the present invention 1) amplifying the quantitative gene by real-time PCR using a primer pair and / or a probe that can amplify the quantitative gene by real-time PCR, and using the composition to identify the candidate standard expression gene contained in the sample Amplifying by real-time PCR; And

2) 단계 1)의 정량 대상 유전자의 발현 정도 및 후보 표준 발현 유전자의 발현 정도를 표준화하는 단계를 포함하는 정량 대상 유전자의 발현량 정량 방법을 제공한다.2) provides a method for quantifying the expression amount of the gene to be quantified, comprising the step of standardizing the expression level of the gene to be quantified in step 1) and the expression level of the candidate standard expression gene.

또한, 본 발명은 상기 방법으로 선별된 후보 표준 발현 유전자의 변동계수(coefficient of variation, CV)를 측정하여 변동계수가 낮은 순으로 표준 발현 유전자를 선별하는 단계를 포함하는 유전자 발현량 측정 시 참고용 유전자의 선별방법을 제공한다.In addition, the present invention is for reference when measuring the gene expression amount comprising the step of selecting the standard expression gene in the order of low coefficient of variation by measuring the coefficient of variation (CV) of the candidate standard expression gene selected by the above method Provide a method for screening genes.

또한, 본 발명은 상기 방법에 의하여 선별된 참고용 유전자를 검출할 수 있는 검출 시약을 포함하는 상기 참고용 유전자의 검출용 조성물을 제공한다.The present invention also provides a composition for detecting the reference gene comprising a detection reagent capable of detecting the reference gene selected by the method.

또한, 본 발명은 1) 피검체로부터 수득된 RNA로부터 cDNA를 수득하는 단계;In addition, the present invention comprises the steps of 1) obtaining cDNA from RNA obtained from the subject;

2) 단계 1)의 cDNA를 주형 DNA로 하여 대상 유전자를 증폭할 수 있는 프라이머쌍 및/또는 프로브를 이용하여 상기 대상 유전자를 실시간 PCR로 증폭하고, 상기 조성물을 이용하여 시료에 포함되어 있는 후보 표준 발현 유전자를 실시간 PCR로 증폭하는 단계; 및,2) Amplification of the target gene by real-time PCR using primer pairs and / or probes capable of amplifying the target gene using the cDNA of step 1) as a template DNA, and the candidate standard included in the sample using the composition. Amplifying the expressed gene by real-time PCR; And,

3) 단계 2)의 대상 유전자의 발현 정도 및 후보 표준 발현 유전자의 발현 정도를 표준화하는 단계를 포함하는 대상 유전자의 발현량 정량 방법을 제공한다.3) It provides a method for quantifying the expression level of the target gene comprising the step of normalizing the expression level of the target gene of step 2) and the expression of the candidate standard expression gene.

아울러, 본 발명은 1) 피검체로부터 수득된 게놈 DNA를 주형 DNA로 하여 대상 유전자를 증폭할 수 있는 프라이머쌍 및/또는 프로브를 이용하여 상기 대상 유전자를 실시간 PCR로 증폭하고, 상기 조성물을 이용하여 시료에 포함되어 있는 후 보 표준 발현 유전자를 실시간 PCR로 증폭하는 단계; 및,In addition, the present invention 1) using a primer pair and / or a probe that can amplify a target gene using genomic DNA obtained from the subject as a template DNA amplify the target gene by real-time PCR, using the composition Amplifying the candidate standard expression gene contained in the sample by real-time PCR; And,

2) 단계 1)의 대상 유전자의 발현 정도 및 후보 표준 발현 유전자의 발현 정도를 표준화하는 단계를 포함하는 대상 유전자의 게놈 DNA 내의 증폭여부 감별 방법을 제공한다.2) Provides a method for discriminating whether amplification in the genomic DNA of the target gene comprising the step of normalizing the expression level of the target gene of step 1) and the expression of the candidate standard expression gene.

이하 본 발명에서 사용한 용어를 설명한다.Hereinafter, terms used in the present invention will be described.

용어 "후보 표준 발현 유전자"는 본 발명의 방법에 의해 선별된 것으로, 다양한 조직에서 동시에 발현되는 유지 유전자(housekeeping gene, HKG)의 특성을 갖는 유전자를 의미한다.The term “candidate standard expression gene” is selected by the method of the present invention and means a gene having the characteristics of a housekeeping gene (HKG) expressed simultaneously in various tissues.

용어 "참고용 유전자"는 "후보 표준 발현 유전자" 중 선택된 것으로, 세포 내의 대부분의 전사체와 비슷한 낮은 발현량 및 낮은 발현량 변동을 보이는 특성을 갖는 유전자로써, 본 명세서에서 용어 "표준 발현 유전자" 또는 "내인성 표준 발현 유전자"와 동일한 의미로 사용된다.The term "reference gene" is selected among the "candidate standard expression genes" and is a gene having characteristics showing low expression amount and low expression variation similar to most transcripts in a cell, and as used herein, the term "standard expression gene" Or "endogenous standard expression gene".

이하 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.

본 발명은 The present invention

2) 단계 1)의 유전자 발현량과 제로비율을 이용하여 여러 조직에서 항상 발현되는 유전자를 선별하는 단계를 포함하는 후보 표준 발현 유전 자(Endogenous Reference Genes; ERG)의 선별방법을 제공한다.2) Provides a method for screening candidate endogenous reference genes (ERGs), including selecting genes that are always expressed in various tissues using the gene expression amount and zero ratio of step 1).

내인성 표준 발현 유전자(Endogenous Reference Genes; ERG)는 다른 시료 간의 비교적 정확한 유전자 발현 비교를 위해 mRNA 발현량의 표준화에 가장 보편적으로 사용되어 온 유전자이다. RT-PCR(reverse transcriptase polymerase chain reaction), qRT-PCR(quantitative real time PCR), 유전자발현계열분석(serial analysis of gene expression: 이하 SAGE) 및 마이크로어레이(microarray)에 이르는 유전자 발현 분석법들에 내인성 표준 발현 유전자를 보편적으로 사용하고 있다. 내인성 표준 발현 유전자로써 통상적으로 활용되는 글리세르알데히드삼인산 탈수소효소(glyceraldehyde-3-phosphate dehydrogenase: 이하, GAPDH) 및 β-액틴(β-actin: 이하 ACTB)의 유전자들은 상기 유전자들이 유래의 다양성에 상관없이 시료 간에 일정한 수준으로 발현되며 실험적 처리에 의해 변하지 않을 것이라는 가정하에 적절한 검증 없이 사용돼 오고 있다. 그러나, 조직 및 세포 종류에 따라 다르며 시료의 처리, 발생학적 단계(developmental stage) 및 병리학적 상태(pathological states)를 포함한 실험 조건들에 의해 조절될 수 있다는 사실은 이미 잘 알려져 있다.Endogenous Reference Genes (ERGs) are the most commonly used genes for standardizing mRNA expression levels for comparatively accurate gene expression comparisons between different samples. Endogenous standards for gene expression assays ranging from reverse transcriptase polymerase chain reaction (RT-PCR), quantitative real time PCR (qRT-PCR), serial analysis of gene expression (SAGE) and microarray Expression genes are commonly used. The genes of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin (ACTB) commonly used as endogenous standard expression genes correlate with the diversity from which these genes are derived. It has been used without proper validation under the assumption that it is expressed at a constant level between samples and will not be changed by experimental treatment. However, it is well known that it depends on tissue and cell type and can be controlled by experimental conditions, including treatment of samples, developmental stages and pathological states.

이에, 본 발명자들은 인체의 여러 조직에서 비슷한 수준으로 발현하는 유지 유전자(housekeeping gene, HKG) 탐색을 위해, 먼저, 데이터 세트를 구성하고자 공개 CGAP 사이트(The Cancer Genome Project, //cgap.nci.nih.gov/)로부터 EST(expressed sequence tag) 및 SAGE 인간 유전자 발현데이터와 Affymetrix Human Genome U133(HG-U133) 어레이 세트를 기반으로 제작된 Gene Logic 사의 GeneExpress Oncology Datasuite^TM으로부터 마이크로어레이 유전자 발현 자료를 입수하였다. 상기의 데이터 베이스를 통합하여 데이터 세트를 구성하였으나 활용 가능한 데이터 베이스는 이에 한정되지 않는다. 데이터 세트로부터 입수한 발현자료를 이용하여 하기 <수학식 1> 및 <수학식 2>에 의해 발현량을 결정하였다. 즉 하기 수학식 1로는 특정한 라이브러리 내에서의 특정한 유전자의 EST(expressed sequence tag) 발현 수준을 알 수 있으며, 하기 수학식 2로는 특정한 라이브러리 내에서의 특정한 유전자의 SAGE 발현 수준을 알 수 있다.Thus, the present inventors, in order to search for a housekeeping gene (HKG) that is expressed at similar levels in various tissues of the human body, first, to construct a data set, the public CGAP site (The Cancer Genome Project, //cgap.nci.nih microarray gene expression data were obtained from GeneLogic's GeneExpress Oncology Datasuite ^™ based on the EST (expressed sequence tag) and SAGE human gene expression data and the Affymetrix Human Genome U133 (HG-U133) array set. . Although a data set is constructed by integrating the above databases, the available databases are not limited thereto. Using the expression data obtained from the data set, the expression amount was determined by the following Equation 1 and Equation 2. That is, Equation 1 below shows the expression level of an EST (expressed sequence tag) of a specific gene in a specific library, and Equation 2 below shows the SAGE expression level of a specific gene in a specific library.

본 발명자들은 상이한 데이터 베이스의 데이터를 통합적으로 분석하여 공통사항을 도출하기 위해 새로이 '제로비율'을 도입하여 특정 유전자가 여러 조직에 걸쳐 대부분의 상황에서 발현되는 유지 유전자(housekeeping gene, HKG)일 가능성을 측정하였다.The present inventors introduced a new 'zero ratio' in order to collectively analyze data from different databases to derive commonalities, so that a specific gene is a housekeeping gene (HKG) expressed in most situations across multiple tissues. Was measured.

제로비율이란 특정 유전자가 발현되지 않은 조직의 개수를 총 조직의 개수로 나눈 것으로, 제로비율이 낮을수록 유지 유전자(HKG)일 가능성이 높다고 할 수 있다. 상기의 '제로비율' 개념을 활용하여 EST, 짤막한 절편의 SAGE(serial analysis of gene expression short; ShortSAGE) 및 긴 절편의 SAGE(serial analysis of gene expression long; LongSAGE) 데이터세트에서 공통적으로 제로비율이 낮은 유전자들을 선택하여 이를 2,087개의 유전자로 분류하였다. 상기 방법으로 분류된 2,087개의 유전자를 하기 "후보 표준 발현 유전자" 또는 "후보 ERG"라고도 할 수 있다. 상기에서 제로비율이 낮다는 것은 EST에서는 제로비율이 0.4, ShortSAGE에서는 제로비율이 0.1, LongSAGE에서는 제로비율이 0.3 보다 작음을 의미한다. 다른 데이터 베이스인 마이크로어레이(Affymetrix HG-U133, CA) 데이터세트에서 EST와 SAGE 데이터세트에서 선별된 2,087개의 유전자의 평균 유전자 발현량 및 변동계수(coefficient of variation, CV)(%)를 구하였다. 상기 2,087개의 유전자 중 1,990개의 유니젠 클러스터(5,238개의 프로브 세트, 5,317 절편)에 대한 발현 데이터가 얻어졌다.The zero ratio is the number of tissues in which a specific gene is not expressed divided by the total number of tissues. The lower the zero ratio, the higher the probability of being a maintenance gene (HKG). ZE, the SAGE (serial analysis of gene expression short; ShortSAGE) of short fragments and the SAGE (serial analysis of gene expression long (LongSAGE) dataset of long fragments) have low zero ratio in common Genes were selected and classified into 2,087 genes. The 2,087 genes classified by this method may also be referred to as "candidate standard expression genes" or "candidate ERGs". The low zero ratio means that the zero ratio is 0.4 in EST, the zero ratio is 0.1 in ShortSAGE, and the zero ratio is 0.3 in LongSAGE. The average gene expression and coefficient of variation (CV) (%) of 2,087 genes selected from the EST and SAGE datasets were obtained from another database, the microarray (Affymetrix HG-U133, CA) dataset. Expression data for 1,990 Unigen clusters (5,238 probe sets, 5,317 fragments) of the 2,087 genes were obtained.

그 결과, 4개의 데이터세트[EST(expressed sequence tag), ShortSAGE, LongSAGE 및 마이크로어레이 데이터세트] 간 평균 유전자 발현량은 유의한 상관관계를 나타냈다(도 3 참조). CV의 경우 비록 유의성을 보여주긴 했지만 평균 유전자 발현량보다는 데이터세트 간에 낮은 상관 관계를 보여주었다(도 4 참조).As a result, the mean gene expression amount between the four datasets (expressed sequence tag (EST), ShortSAGE, LongSAGE and microarray dataset) showed a significant correlation (see FIG. 3). In the case of CV, although it showed significance, there was a low correlation between the datasets rather than the average gene expression (see FIG. 4).

이와 더불어, 각 데이터세트의 후보 ERG와 비-ERG의 유전자 발현을 비교한 결과에서는 후보ERG의 평균 유전자 발현량이 비-ERG 보다 4개의 데이터세트 모두에 서 유의하게 높았다(p<0.0001)(도 5 참조).In addition, comparing the gene expression of candidate ERG and non-ERG in each dataset, the mean gene expression of candidate ERG was significantly higher in all four datasets than non-ERG (p <0.0001) (FIG. 5). Reference).

상기 선별된 후보 표준 발현 유전자는 Accession No. Hs 120(PRDX6), Accession No. Hs 142(SULT1A1), Accession No. Hs 202(BZRP), Accession No. Hs 429(ATP5G3), Accession No. Hs 695(CSTB), Accession No. Hs 808(HNRPF), Accession No. Hs 861(MAPK3), Accession No. Hs 1063(SNRPC), Accession No. Hs 1103(TGFB1), Accession No. Hs 2430(TCFL1), Accession No. Hs 2533(ALDH9A1), Accession No. Hs 2795(LDHA), Accession No. Hs 2853(PCBP1), Accession No. Hs 3100(KARS), Accession No. Hs 3254(MRPL23), Accession No. Hs 3353(G3BP), Accession No. Hs 3416(ADFP), Accession No. Hs 439(STOML2), Accession No. Hs 3530(FUSIP1), Accession No. Hs 3989(PLXNB2), Accession No. Hs 4055(KLF6), Accession No. Hs 4742(GPAA1), Accession No. Hs 4747(DKC1), Accession No. Hs 4766(FAM32A), Accession No. Hs 4859(CCNL1), Accession No. Hs 4997(RBM23), Accession No. Hs 4998(TMOD3), Accession No. Hs 5062(TM4SF8), Accession No. Hs 5086(MGC10433), Accession No. Hs 5120(DNCL1), Accession No. Hs 5158(ILK), Accession No. Hs 5245(FLJ20643), Accession No. Hs 5258(MAGED1), Accession No. Hs 5268(ZDHHC4), Accession No. Hs 5298(ADIPOR1), Accession No. Hs 5308(UBA52), Accession No. Hs 5324(C2orf25), Accession No. Hs 5345(RNPEPL1), Accession No. Hs 5662(GNB2L1), Accession No. Hs 5710(CREG1), Accession No. Hs 5719(CNAP1), Accession No. Hs 5912(FBXO7), Accession No. Hs 5947(RAB8A), Accession No. Hs 6396(JTB), Accession No. Hs 6454(RGS19IP1), Accession No. Hs 6459(GPR172A), Accession No. Hs 6551(ATP6AP1), Accession No. Hs 6891(SFRS6), Accession No. Hs 7101(ANAPC5), Accession No. Hs 7236(NOSIP), Accession No. Hs 7476(ATP6V0B), Accession No. Hs 7527(DKFZP566E144), Accession No. Hs 7744(NDUFV1), Accession No. Hs 7753(CALU), Accession No. Hs 7768(FIBP), Accession No. Hs 7862(PNRC2), Accession No. Hs 7910(RYBP), Accession No. Hs 7917(HIG1), Accession No. Hs 8102(RPS20), Accession No. Hs 8372(UQCR), Accession No. Hs 8737(WDR6), Accession No. Hs 8752(TMEM4), Accession No. Hs 8765(DDX42), Accession No. Hs 8859(CANT1), Accession No. Hs 8867(CYR61), Accession No. Hs 9003(FLJ13868), Accession No. Hs 9015(MGC52000), Accession No. Hs 9043(C14orf120), Accession No. Hs 9234(NIFIE14), Accession No. Hs 9235(NME4), Accession No. Hs 9527(C2orf28), Accession No. Hs 9534(SEC11L1), Accession No. Hs 9573(ABCF1), Accession No. Hs 9589(UBQLN1), Accession No. Hs 9788(NDFIP1), Accession No. Hs 9825(CGI-128), Accession No. Hs 9857(DCXR), Accession No. Hs 10326(COPE), Accession No. Hs 10842(RAN), Accession No. Hs 10848(BMS1L), Accession No. Hs 11125(SPCS1), Accession No. Hs 11184(UBE2R2), Accession No. Hs 11223(IDH1), Accession No. Hs 11355(TMPO), Accession No. Hs 11463(UMP-CMPK), Accession No. Hs 12013(ABCE1), Accession No. Hs 12084(TUFM), Accession No. Hs 12102(SNX3), Accession No. Hs 12107(BC-2), Accession No. Hs 12109(WDR39), Accession No. Hs 12144(KIAA1033), Accession No. Hs 12152(SRPRB), Accession No. Hs 12272(BECN1), Accession No. Hs 12341(ADAR), Accession No. Hs 12457(NUP133), Accession No. Hs 12865(NSFL1C), Accession No. Hs 13662(MGC5508), Accession No. Hs 14317(NOLA3), Accession No. Hs 14333(FLJ10349), Accession No. Hs 14745(C10orf9), Accession No. Hs 14839(POLR2G), Accession No. Hs 14846(SLC7A1), Accession No. Hs 14894(TGOLN2), Accession No. Hs 15277(C16orf33), Accession No. Hs 15591(COPS6), Accession No. Hs 15738(RAB7), Accession No. Hs 16059(HSPC009), Accession No. Hs 16130(E2-230K), Accession No. Hs 16349(KIAA0431), Accession No. Hs 17118(FLJ11730), Accession No. Hs 17250(MGC4767), Accession No. Hs 17680(FUCA2), Accession No. Hs 17731(FLJ12892), Accession No. Hs 17883(PPM1G), Accession No. Hs 18069(LGMN), Accession No. Hs 18128(C20orf44), Accession No. Hs 18349(MRPL15), Accession No. Hs 19673(MAF1), Accession No. Hs 20013(P29), Accession No. Hs 20107(KNS2), Accession No. Hs 20157(CDK5RAP3), Accession No. Hs 20521(HRMT1L2), Accession No. Hs 20529(LOC127262), Accession No. Hs 20573(IGF1R), Accession No. Hs 20716(TIMM17A), Accession No. Hs 22393(DENR), Accession No. Hs 22543(UBE3A), Accession No. Hs 22546(CYBASC3), Accession No. Hs 22616(KIAA0664), Accession No. Hs 23033(LOC92912), Accession No. Hs 23111(FARSLA), Accession No. Hs 23978(SAFB), Accession No. Hs 24301(POLR2E), Accession No. Hs 24379(TRAPPC1), Accession No. Hs 24601(FBLN1), Accession No. Hs 24950(RGS5), Accession No. Hs 25155(NET1), Accession No. Hs 25450(SLC29A1), Accession No. Hs 25723(MTVR1), Accession No. Hs 26010(PFKP), Accession No. Hs 26023(FOXJ3), Accession No. Hs 26136(MGC14156), Accession No. Hs 26232(MAN2C1), Accession No. Hs 26403(GSTZ1), Accession No. Hs 26518(TM4SF7), Accession No. Hs 27222(NOLA2), Accession No. Hs 28491(SAT), Accession No. Hs 28914(APRT), Accession No. Hs 29203(GBL), Accession No. Hs 29665(CLSTN1), Accession No. Hs 30011(MGC2963), Accession No. Hs 30026(HSPC182), Accession No. Hs 30345(TRAP1), Accession No. Hs 30954(PMVK), Accession No. Hs 31053(CKAP1), Accession No. Hs 31334(C20orf14), Accession No. Hs 31387(DKFZP564J0123), Accession No. Hs 34045(CDCA4), Accession No. Hs 34576(TAX1BP1), Accession No. Hs 34906(BLOC1S2), Accession No. Hs 35052(TEGT), Accession No. Hs 35828(MARK3), Accession No. Hs 36587(PPP1R7), Accession No. Hs 36927(HSPH1), Accession No. Hs 37616(STRA13), Accession No. Hs 37916(DPP7), Accession No. Hs 42806(Cab45), Accession No. Hs 43297(MTPN), Accession No. Hs 47062(POLR2I), Accession No. Hs 50098(NDUFA4), Accession No. Hs 50308(HIP2), Accession No. Hs 50425(TEBP), Accession No. Hs 53066(HSPBP1), Accession No. Hs 54277(FAM50A), Accession No. Hs 54457(CD81), Accession No. Hs 54642(MAT2B), Accession No. Hs 54649(RY1), Accession No. Hs 55682(EIF3S7), Accession No. Hs 55847(MRPL51), Accession No. Hs 58488(CTNNAL1), Accession No. Hs 58992(SMC4L1), Accession No. Hs 59486(HSDL2), Accession No. Hs 61812(PTPN12), Accession No. Hs 65234(DDX27), Accession No. Hs 65238(RNF40), Accession No. Hs 66048(BPY2IP1), Accession No. Hs 66915(C22orf16), Accession No. Hs 68714(SFRS1), Accession No. Hs 9293(HEXB), Accession No. Hs 69554(RNF126), Accession No. Hs 69855(UNR), Accession No. Hs 71465(SQLE), Accession No. Hs 71787(MRPS7), Accession No. Hs 73527(CSNK2B), Accession No. Hs 73722(APEX1), Accession No. Hs 73799(GNAI3), Accession No. Hs 73965(SFRS2), Accession No. Hs 74047(ETFB), Accession No. Hs 74050(FVT1), Accession No. Hs 74137(TMP21), Accession No. Hs 74375(DVL1), Accession No. Hs 74405(YWHAQ), Accession No. Hs 74471(GJA1), Accession No. Hs 74563(OAZ2), Accession No. Hs 74564(SSR2), Accession No. Hs 74576(GDI1), Accession No. Hs 75056(TIMM13), Accession No. Hs 75061(MARCKSL1), Accession No. Hs 75066(TSN), Accession No. Hs 75087(FASTK), Accession No. Hs 75117(ILF2), Accession No. Hs 75133(TFAM), Accession No. Hs 75139(ARFIP2), Accession No. Hs 75189(DAP), Accession No. Hs 75227(NDUFA9), Accession No. Hs 75243(BRD2), Accession No. Hs 75249(ARL6IP), Accession No. Hs 75254(IRF3), Accession No. Hs 75318(TUBA1), Accession No. Hs 75348(PSME1), Accession No. Hs 75438(QDPR), Accession No. Hs 75527(ADSL), Accession No. Hs 75724(COPB2), Accession No. Hs 75798(C20orf111), Accession No. Hs 75841(C12orf8), Accession No. Hs 75890(MBTPS1), Accession No. Hs 75914(RNP24), Accession No. Hs 76111(DAG1), Accession No. Hs 76394(ECHS1), Accession No. Hs 76480(UBL4), Accession No. Hs 76662(ZDHHC16), Accession No. Hs 76686(GPX1), Accession No. Hs 76847(GANAB), Accession No. Hs 77060(PSMB6), Accession No. Hs 77269(GNAI2), Accession No. Hs 77313(CDK10), Accession No. Hs 77422(PLP2), Accession No. Hs 77558(HMGN3), Accession No. Hs 77578(USP9X), Accession No. Hs 77793(CSK), Accession No. Hs 77897(SF3A3), Accession No. Hs 77961(HLA-B), Accession No. Hs 77978(DKFZp761I2123), Accession No. Hs 78466(PSMD8), Accession No. Hs 78601(UROD), Accession No. Hs 78771(PGK1), Accession No. Hs 78880(ILVBL), Accession No. Hs 78888(DBI), Accession No. Hs 78989(ADH5), Accession No. Hs 79064(DHPS), Accession No. Hs 79081(PPP1CC), Accession No. Hs 79088(RCN2), Accession No. Hs 79101(CCNG1), Accession No. Hs 79110(NCL), Accession No. Hs 79322(QARS), Accession No. Hs 79335(SMARCD1), Accession No. Hs 79387(PSMC5), Accession No. Hs 79402(POLR2C), Accession No. Hs 79411(RPA2), Accession No. Hs 79625(C20orf149), Accession No. Hs 80545(RPL37), Accession No. Hs 80919(SYPL), Accession No. Hs 80986(ATP5G1), Accession No. Hs 81328(NFKBIA), Accession No. Hs 81424(SUMO1), Accession No. Hs 81848(RAD21), Accession No. Hs 81964(SEC24C), Accession No. Hs 82201(CSNK2A2), Accession No. Hs 82327(GSS), Accession No. Hs 82719(MGC21416), Accession No. Hs 82793(PSMB3), Accession No. Hs 82887(PPP1R11), Accession No. Hs 82890(DAD1), Accession No. Hs 82916(CCT6A), Accession No. Hs 82927(AMPD2), Accession No. Hs 83190(FASN), Accession No. Hs 83347(AAMP), Accession No. Hs 83383(PRDX4), Accession No. Hs 83734(STX4A), Accession No. Hs 83753(SNRPB), Accession No. Hs 83765(DHFR), Accession No. Hs 83916(NDUFA5), Accession No. Hs 84359(GABARAP), Accession No. Hs 84753(FLJ12442), Accession No. Hs 85155(ZFP36L1), Accession No. Hs 85769(ERBP), Accession No. Hs 85962(DERPC), Accession No. Hs 86131(FADD), Accession No. Hs 87752(MSN), Accession No. Hs 89545(PSMB4), Accession No. Hs 89643(TKT), Accession No. Hs 89649(EPHX1), Accession No. Hs 89781(UBTF), Accession No. Hs 89864(SKIV2L), Accession No. Hs 90061(PGRMC1), Accession No. Hs 90093(HSPA4), Accession No. Hs 90107(ADRM1), Accession No. Hs 90443(NDUFS8), Accession No. Hs 91142(KHSRP), Accession No. Hs 91531(MLLT6), Accession No. Hs 93659(ERP70), Accession No. Hs 93832(LOC54499), Accession No. Hs 95577(CDK4), Accession No. Hs 96530(COX11), Accession No. Hs 96852(FLJ21128), Accession No. Hs 96996(HNRPA0), Accession No. Hs 97616(SH3GL1), Accession No. Hs 97887(RCN1), Accession No. Hs 98751(FUBP3), Accession No. Hs 98791(ACTR1B), Accession No. Hs 102696(MCTS1), Accession No. Hs 102798(PSMA1), Accession No. Hs 103561(ARL6IP4), Accession No. Hs 103834(MGC5576), Accession No. Hs 104839(TIMP2), Accession No. Hs 105547(NPDC1), Accession No. Hs 106185(RALGDS), Accession No. Hs 106876(ATP6V0D1), Accession No. Hs 106909(ANAPC13), Accession No. Hs 107003(CCNB1IP1), Accession No. Hs 107101(FLJ31031), Accession No. Hs 107387(C7orf20), Accession No. Hs 107393(C3orf4), Accession No. Hs 108029(SH3BGRL), Accession No. Hs 108080(CSRP1), Accession No. Hs 108371(E2F4), Accession No. Hs 108408(APH-1A), Accession No. Hs 108957(RPS27L), Accession No. Hs 108969(PTD008), Accession No. Hs 109051(SH3BGRL3), Accession No. Hs 109052(C14orf2), Accession No. Hs 109672(SIAT7F), Accession No. Hs 109798(C6orf48), Accession No. Hs 110695(SF3B5), Accession No. Hs 110849(ESRRA), Accession No. Hs 111286(MRPS11), Accession No. Hs 111577(ITM2C), Accession No. Hs 111801(ARS2), Accession No. Hs 112058(SIVA), Accession No. Hs 112318(TOMM7), Accession No. Hs 112955(NUDT5), Accession No. Hs 114033(SSR1), Accession No. Hs 114286(CD9), Accession No. Hs 114412(TXNL1), Accession No. Hs 115474(RFC3), Accession No. Hs 115792(EXOSC7), Accession No. Hs 116448(GLS), Accession No. Hs 117176(PABPN1), Accession No. Hs 117715(ST5), Accession No. Hs 118110(BST2), Accession No. Hs 118400(FSCN1), Accession No. Hs 118463(PNPLA2), Accession No. Hs 118638(NME1), Accession No. Hs 118722(FUT8), Accession No. Hs 118964(p66alpha), Accession No. Hs 118983(GSDMDC1), Accession No. Hs 19177(ARF3), Accession No. Hs 119192(H2AFZ), Accession No. Hs 119251(UQCRC1), Accession No. Hs 119591(AP2S1), Accession No. Hs 119598(RPL3), Accession No. Hs 120323(DNAPTP6), Accession No. Hs 121088(NUP153), Accession No. Hs 121549(CDIPT), Accession No. Hs 122363(WIPI-2), Accession No. Hs 122523(SND1), Accession No. Hs 124126(ARPC1A), Accession No. Hs 124147(FBXL11), Accession No. Hs 124246(C10orf119), Accession No. Hs 124366(BBX), Accession No. Hs 125113(CCT8), Accession No. Hs 125867(EVL), Accession No. Hs 125898(GNAS), Accession No. Hs 126497(AEBP2), Accession No. Hs 126774(RAMP), Accession No. Hs 126938(NAPA), Accession No. Hs 127092(DHX38), Accession No. Hs 127249(EAP30), Accession No. Hs 127386(MAMDC2), Accession No. Hs 127764(RAB5C), Accession No. Hs 128065(CTSC), Accession No. Hs 128199(SEPT11), Accession No. Hs 128548(WDR1), Accession No. Hs 129634(CINP), Accession No. Hs 129673(EIF4A1), Accession No. Hs 130031(TRIO), Accession No. Hs 130098(DDX23), Accession No. Hs 130293(CROP), Accession No. Hs 130413(TM9SF2), Accession No. Hs 131226(BNIP3L), Accession No. Hs 132497(PRNPIP), Accession No. Hs 132513 HSD17B12), Accession No. Hs 133892(TPM1), Accession No. Hs 134074(SLC35E1), Accession No. Hs 134688(PSMD13), Accession No. Hs 135406(CEBPZ), Accession No. Hs 136905(UREB1), Accession No. Hs 136947(RALY), Accession No. Hs 137510(NCOR2), Accession No. Hs 138860(ARHGAP1), Accession No. Hs 139896(MAEA), Accession No. Hs 140452(M6PRBP1), Accession No. Hs 142442(HP1-BP74), Accession No. Hs 143187(DDX49), Accession No. Hs 143766(DRPLA), Accession No. Hs 143873(S100A10), Accession No. Hs 144058(EBSP), Accession No. Hs 144468(MGC3234), Accession No. Hs 144835(EEF1G), Accession No. Hs 144868(VTI1B), Accession No. Hs 144941(MUF1), Accession No. Hs 144949(ZNF313), Accession No. Hs 144980(SCAMP4), Accession No. Hs 145049(PLEKHM2), Accession No. Hs 145442(MAP2K1), Accession No. Hs 145575(UBL3), Accession No. Hs 146070(TPM3), Accession No. Hs 146393(HERPUD1), Accession No. Hs 146602(QP-C), Accession No. Hs 146804(SPIN), Accession No. Hs 146806(CUL1), Accession No. Hs 147433(PCNA), Accession No. Hs 148078(RBAF600), Accession No. Hs 148272(CCM2), Accession No. Hs 148330(ARF4), Accession No. Hs 148340(PTPRG), Accession No. Hs 148670(RHOBTB1), Accession No. Hs 149004(FBXO31), Accession No. Hs 149957(RPS6KA1), Accession No. Hs 149983(PEX14), Accession No. Hs 150107(BIRC6), Accession No. Hs 150540(BC002942), Accession No. Hs 150580(SUI1), Accession No. Hs 150837(TXNDC5), Accession No. Hs 151134(OXA1L), Accession No. Hs 151220(KIAA0992), Accession No. Hs 151413(GMFB), Accession No. Hs 151787(U5-116KD), Accession No. Hs 152536(p44S10), Accession No. Hs 153177(RPS28), Accession No. Hs 154023(TXNDC4), Accession No. Hs 154073(SLC35B1), Accession No. Hs 155165(ZFPL1), Accession No. Hs 155218(HNRPUL1), Accession No. Hs 155396(NFE2L2), Accession No. Hs 155829(KIAA0676), Accession No. Hs 156171(PSMC6), Accession No. Hs 156367(RPS29), Accession No. Hs 156667(KIAA1536), Accession No. Hs 157160(MRPS34), Accession No. Hs 157351(PTD004), Accession No. Hs 157379(H2AFV), Accession No. Hs 157394(HAGH), Accession No. Hs 159014(PRPF4B), Accession No. Hs 159118(AMD1), Accession No. Hs 159130(RAF1), Accession No. Hs 159161(ARHGDIA), Accession No. Hs 159699(FBXO21), Accession No. Hs 159799(THRAP2), Accession No. Hs 160958(CDC37), Accession No. Hs 161357(PDHB), Accession No. Hs 162032(HBP1), Accession No. Hs 162233(CHD4), Accession No. Hs 162877(PACSIN2), Accession No. Hs 163645(MOCS2), Accession No. Hs 163776(UBE2J1), Accession No. Hs 163893(PICALM), Accession No. Hs 165195(VAPA), Accession No. Hs 166011(CTNND1), Accession No. Hs 166204(PHF1), Accession No. Hs 166463(HNRPU), Accession No. Hs 166924(SEC13L1), Accession No. Hs 166975(SFRS5), Accession No. Hs 167535(SRP54), Accession No. Hs 168073(TRPC4AP), Accession No. Hs 168799(METTL3), Accession No. Hs 169611(DIABLO), Accession No. Hs 169718(CNN2), Accession No. Hs 170107(UQCRFS1), Accession No. Hs 170131(NFIC), Accession No. Hs 170553(CNOT7), Accession No. Hs 170622(CFL1), Accession No. Hs 171626(SKP1A), Accession No. Hs 172550(PTBP1), Accession No. Hs 172755(BRP44L), Accession No. Hs 172928(COL1A1), Accession No. Hs 173024(NYREN18), Accession No. Hs 173162(NOC4), Accession No. Hs 173381(DPYSL2), Accession No. Hs 173464(FKBP8), Accession No. Hs 173611(NDUFS2), Accession No. Hs 173705(LOC401152), Accession No. Hs 173724(CKB), Accession No. Hs 174050(EDF1), Accession No. Hs 174195(IFITM2), Accession No. Hs 175473(AK1), Accession No. Hs 175955(YT521), Accession No. Hs 177530(ATP5E), Accession No. Hs 177766(PARP1), Accession No. Hs 178551(RPL8), Accession No. Hs 178728(MBD3), Accession No. Hs 179986(FLOT1), Accession No. Hs 180141(CFL2), Accession No. Hs 180312(MRPS16), Accession No. Hs 180414(HSPA8), Accession No. Hs 180877(H3F3B), Accession No. Hs 180903(384D8-2), Accession No. Hs 180909(PRDX1), Accession No. Hs 180933(CXXC1), Accession No. Hs 181046(DUSP3), Accession No. Hs 181112(MED4), Accession No. Hs 181163(HMGN2), Accession No. Hs 181244(HLA-A), Accession No. Hs 181368(PRPF8), Accession No. Hs 181444(TMEM9), Accession No. Hs 182255(NHP2L1), Accession No. Hs 182626(C22orf5), Accession No. Hs 182885(SLC35B2), Accession No. Hs 183684(EIF4G2), Accession No. Hs 183706(ADD1), Accession No. Hs 183800(RANGAP1), Accession No. Hs 183850(DCTD), Accession No. Hs 183994(PPP1CA), Accession No. Hs 184062(C20orf24), Accession No. Hs 184211(PMPCB), Accession No. Hs 184233(HSPA9B), Accession No. Hs 184492(ELAVL1), Accession No. Hs 185172(GNB2), Accession No. Hs 185597(SPG7), Accession No. Hs 187199(MALAT1), Accession No. Hs 187635(RPS15A), Accession No. Hs 187763(BRD4), Accession No. Hs 187866(SDFR1), Accession No. Hs 187946(SLC20A1), Accession No. Hs 188501(PAFAH1B2), Accession No. Hs 188614(PLEKHA5), Accession No. Hs 188879(RBM6), Accession No. Hs 188882(NUDT3), Accession No. Hs 189075(PTK9), Accession No. Hs 189119(CXXC5), Accession No. Hs 189329(SMURF1), Accession No. Hs 189716(NDUFAB1), Accession No. Hs 189772(CCT2), Accession No. Hs 190028(GSTO1), Accession No. Hs 190086(MRCL3), Accession No. Hs 190334(RAP1A), Accession No. Hs 190384(COPS4), Accession No. Hs 190722(HSPC142), Accession No. Hs 190904(STRN4), Accession No. Hs 191186(TTC17), Accession No. Hs 191346(SEPT7), Accession No. Hs 191518(DHX9), Accession No. Hs 191987(UBE2J2), Accession No. Hs 192316(CDC2L1), Accession No. Hs 192374(TRA1), Accession No. Hs 192425(EIF3S8), Accession No. Hs 193118(RAI17), Accession No. Hs 193163(BIN1), Accession No. Hs 193491(TUBB6), Accession No. Hs 194329(TCEAL4), Accession No. Hs 194718(ZNF265), Accession No. Hs 195464(FLNA), Accession No. Hs 195642(C17orf27), Accession No. Hs 196983(SSFA2), Accession No. Hs 198281(PKM2), Accession No. Hs 199561(RANBP2), Accession No. Hs 199625(HAX1), Accession No. Hs 200063(HDAC7A), Accession No. Hs 200600(SCAMP3), Accession No. Hs 200804(SDCBP), Accession No. Hs 201253(ch-TOG), Accession No. Hs 201390(WDR45L), Accession No. Hs 201712(GLG1), Accession No. Hs 202011(GK001), Accession No. Hs 202085(VDAC1), Accession No. Hs 202166(HNRPH1), Accession No. Hs 202179(SMN2), Accession No. Hs 203099(KIAA0261), Accession No. Hs 203910(SGTA), Accession No. Hs 204041(AHSA1), Accession No. Hs 204773(MEP50), Accession No. Hs 205163(MRPL3), Accession No. Hs 206500(CTTN), Accession No. Hs 206824(MGC71993), Accession No. Hs 208597(CTBP1), Accession No. Hs 209983(STMN1), Accession No. Hs 210469(ELMO2), Accession No. Hs 210532(KIAA0141), Accession No. Hs 211463(DNM2), Accession No. Hs 211594(PSMC4), Accession No. Hs 211914(NDUFS7), Accession No. Hs 212102(TXNDC7), Accession No. Hs 212395(CIZ1), Accession No. Hs 213061(NUCKS), Accession No. Hs 213470(PSMB7), Accession No. Hs 213541, Accession No. Hs 213666(KIAA0460), Accession No. Hs 213724(SUPT16H), Accession No. Hs 216653(FBXO9), Accession No. Hs 220950(FOXO3A), Accession No. Hs 221847(SLC38A2), Accession No. Hs 222510(DAZAP1), Accession No. Hs 223141(DDX21), Accession No. Hs 224607(SDC1), Accession No. Hs 226007(RDH11), Accession No. Hs 226117(H1F0), Accession No. Hs 226755(YWHAH), Accession No. Hs 227067(ATAD3A), Accession No. Hs 227253(TOMM70A), Accession No. Hs 227777(PTP4A1), Accession No. Hs 229641(PC4), Accession No. Hs 231295(PITPNC1), Accession No. Hs 231616(HSPC023), Accession No. Hs 232194(KIAA0174), Accession No. Hs 232543(PDCD4), Accession No. Hs 233458(NFYC), Accession No. Hs 233552(CDC2L5), Accession No. Hs 233952(PSMA7), Accession No. Hs 234521(MAPKAPK3), Accession No. Hs 236030(SMARCC2), Accession No. Hs 237536(MGC20781), Accession No. Hs 237971(XTP3TPA), Accession No. Hs 238839(SCYL1), Accession No. Hs 240170(MGC2731), Accession No. Hs 241336(ATPIF1), Accession No. Hs 241543(POLDIP2), Accession No. Hs 241558(ARIH2), Accession No. Hs 241575(GNPTG), Accession No. Hs 241576(DERL1), Accession No. Hs 241579(SERPINH1), Accession No. Hs 242458(SPG21), Accession No. Hs 242947(DGKI), Accession No. Hs 246112(ASCC3L1), Accession No. Hs 246310(ATP5J), Accession No. Hs 246413(CPNE1), Accession No. Hs 246781(FBXO11), Accession No. Hs 247077(RHOA), Accession No. Hs 247186(FBS1), Accession No. Hs 247975(HSPD1), Accession No. Hs 248267(MPST), Accession No. Hs 248941(TAF9), Accession No. Hs 49600(DLGAP4), Accession No. Hs 250009(ARL10C), Accession No. Hs 250429(SUPT6H), Accession No. Hs 250758(PSMC3), Accession No. Hs 250899(HSBP1), Accession No. Hs 250905(LOC51234), Accession No. Hs 251531(PSMA4), Accession No. Hs 252457(MVD), Accession No. Hs 252713(TTC15), Accession No. Hs 252967 DKFZp566C0424), Accession No. Hs 253726(PAPOLA), Accession No. Hs 253903(STOM), Accession No. Hs 254042(BAT1), Accession No. Hs 255015(VPS24), Accession No. Hs 255093(PFKL), Accession No. Hs 255932(XRN2), Accession No. Hs 255935(BTG1), Accession No. Hs 255973(CRI1), Accession No. Hs 256301(MGC13170), Accession No. Hs 256549(NUBP2), Accession No. Hs 257008(PLD3), Accession No. Hs 257341(SAV1), Accession No. Hs 57761(SH3BP5), Accession No. Hs 258551(DNPEP), Accession No. Hs 258563(FEZ2), Accession No. Hs 258798 C10orf86), Accession No. Hs 259461(PALM2-AKAP2), Accession No. Hs 260603(PIP5K2B), Accession No. Hs 262823(FLJ10326), Accession No. Hs 265829(ITGA3), Accession No. Hs 268488(KIAA1185), Accession No. Hs 268530(GPS1), Accession No. Hs 268742(C13orf12), Accession No. Hs 268849(GLO1), Accession No. Hs 268939(MATR3), Accession No. Hs 269528(MAK3), Accession No. Hs 269577(PTPRA), Accession No. Hs 269782(GNAQ), Accession No. Hs 269944(MTCH2), Accession No. Hs 270291(ACTN4), Accession No. Hs 270428(SUCLG1), Accession No. Hs 270525(LASS5), Accession No. Hs 270869(ZNF410), Accession No. Hs 271135(ATP5C1), Accession No. Hs 271695(NOB1P), Accession No. Hs 272062(PTPRF), Accession No. Hs 272168(TDE1), Accession No. Hs 272630(ATP6V1D), Accession No. Hs 272927(SEC23A), Accession No. Hs 273077(TMEM14B), Accession No. Hs 274184(TFE3), Accession No. Hs 274772(C15orf15), Accession No. Hs 274873(CARS), Accession No. Hs 275243(S100A6), Accession No. Hs 275775(SEPP1), Accession No. Hs 275865(PCNP), Accession No. Hs 276878(NUP93), Accession No. Hs 277035(MGLL), Accession No. Hs 277517(C11orf2), Accession No. Hs 278186(ARHGEF1), Accession No. Hs 278362(MEA), Accession No. Hs 278426(PDAP1), Accession No. Hs 278429(C9orf78), Accession No. Hs 278500(GNPDA1), Accession No. Hs 278569(SNX17), Accession No. Hs 278573(CD59), Accession No. Hs 278721(SLC39A7), Accession No. Hs 279061(C17orf25), Accession No. Hs 279245(TACC1), Accession No. Hs 279257(PCMT1), Accession No. Hs 279413(POLD1), Accession No. Hs 279529(PX19), Accession No. Hs 279583(DREV1), Accession No. Hs 279623(SEPX1), Accession No. Hs 279640(TPR), Accession No. Hs 279652(MRPL4), Accession No. Hs 279669(TUBG1), Accession No. Hs 279696(SUMF2), Accession No. Hs 279806(DDX5), Accession No. Hs 79836(COMMD9), Accession No. Hs 279920(YWHAB), Accession No. Hs 279929(TMED9), Accession No. Hs 80202(SBF1), Accession No. Hs 280342(PRKAR1A), Accession No. Hs 280378(SNRPB2), Accession No. Hs 282410(CALM1), Accession No. Hs 282700(SPCS2), Accession No. Hs 282901(RNPC2), Accession No. Hs 282998(RBM9), Accession No. Hs 283111(C14orf124), Accession No. Hs 283454(BNIP2), Accession No. Hs 283521(RHEB), Accession No. Hs 283610(APG4B), Accession No. Hs 283652(IDI1), Accession No. Hs 283739(UBQLN4), Accession No. Hs 284208(ANKRD25), Accession No. Hs 284279(HMOX2), Accession No. Hs 284286(MRPS24), Accession No. Hs 284491(PDXK), Accession No. Hs 285354(MAX), Accession No. Hs 285976(LASS2), Accession No. Hs 286221(ARF1), Accession No. Hs 286226(MYO1C), Accession No. Hs 288193(KPNA4), Accession No. Hs 288856(PFDN5), Accession No. Hs 288969(HSCARG), Accession No. Hs 289008(C6orf68), Accession No. Hs 289092(COTL1), Accession No. Hs 289123(DCTN2), Accession No. Hs 289271(CYC1), Accession No. Hs 290243(GBF1), Accession No. Hs 290404(SLC25A3), Accession No. Hs 290758(DDB1), Accession No. Hs 291587(ARID1B), Accession No. Hs 292026(EIF4E2), Accession No. Hs 292063(EIF4B), Accession No. Hs 292078(LARP), Accession No. Hs 292265(ZMYND11), Accession No. Hs 292457, Accession No. Hs 292493(G22P1), Accession No. Hs 292524(CCNH), Accession No. Hs 292579(PTDSS1), Accession No. Hs 293563(FLJ12666), Accession No. Hs 295917(ATP6V1B2), Accession No. Hs 297324(TIMP3), Accession No. Hs 298198(CKLFSF3), Accession No. Hs 298280(ATP5A1), Accession No. Hs 298654(DUSP6), Accession No. Hs 299002(FBL), Accession No. Hs 299055(GDI2), Accession No. Hs 300141(RPL39), Accession No. Hs 300684(RCP9), Accession No. Hs 300772(TPM2), Accession No. Hs 300816 RAB1B), Accession No. Hs 300834(GALNT2), Accession No. Hs 301404(RBM3), Accession No. Hs 301412(Ufc1), Accession No. Hs 302742(MRPS6), Accession No. Hs 302903(UBE2I), Accession No. Hs 303676(G3BP2), Accession No. Hs 304192(DSTN), Accession No. Hs 304682(CST3), Accession No. Hs 306123(MAGEF1), Accession No. Hs 306242(RANBP9), Accession No. Hs 306329(ZA20D3), Accession No. Hs 306425(IBTK), Accession No. Hs 308122(ITPK1), Accession No. Hs 308340(NUP188), Accession No. Hs 308709(GRP58), Accession No. Hs 309090(SFRS7), Accession No. Hs 309231(C6orf153), Accession No. Hs 309641(RNF11), Accession No. Hs 309753(STARD3NL), Accession No. Hs 309849(C14orf159), Accession No. Hs 310542(TOMM40), Accession No. Hs 310645(RAB1A), Accession No. Hs 311072(MRPS35), Accession No. Hs 311346(CMAS), Accession No. Hs 311609(DDX39), Accession No. Hs 311640(RPS27A), Accession No. Hs 312098(ADAM15), Accession No. Hs 313847(TXNDC11), Accession No. Hs 314263(BAZ2A), Accession No. Hs 314359(EIF3S12), Accession No. Hs 315177(IFRD2), Accession No. Hs 315230(GC20), Accession No. Hs 319334(NASP), Accession No. Hs 321391(MGC4549), Accession No. Hs 321541(RAB11A), Accession No. Hs 323363(APG9L1), Accession No. Hs 323489(FLJ20758), Accession No. Hs 324250(NDUFB2), Accession No. Hs 324844(VKORC1), Accession No. Hs 325650(EHD2), Accession No. Hs 326387(MORF4L2), Accession No. Hs 330384(CORO1C), Accession No. Hs 331431(SCC-112), Accession No. Hs 333388(EEF1D), Accession No. Hs 333579(HSPC152), Accession No. Hs 333786(PSMA2), Accession No. Hs 333823(MRPL13), Accession No. Hs 334017(K-ALPHA-1), Accession No. Hs 334479(TRAF7), Accession No. Hs 334534(GNS), Accession No. Hs 334587(RBPMS), Accession No. Hs 334713(BMSC-UbP), Accession No. Hs 334851(LASP1), Accession No. Hs 334868(PPP2R5E), Accession No. Hs 335003(ANKRD11), Accession No. Hs 335057(SEPT2), Accession No. Hs 335163(KIAA1102), Accession No. Hs 335918(FDPS), Accession No. Hs 337295(STIP1), Accession No. Hs 337766(TXNRD1), Accession No. Hs 339278(COPB), Accession No. Hs 339639(COX7A2L), Accession No. Hs 339697(GRINA), Accession No. Hs 343911(EI24), Accession No. Hs 345694(KCMF1), Accession No. Hs 346868(EBNA1BP2), Accession No. Hs 348418(DR1), Accession No. Hs 349656(SCARB2), Accession No. Hs 350194(ZMAT2), Accession No. Hs 350229(CASC3), Accession No. Hs 350268(IRF2BP2), Accession No. Hs 350364(C9orf10OS), Accession No. Hs 350927(SLC25A6), Accession No. Hs 351099(FLJ10241), Accession No. Hs 351296(LOC51035), Accession No. Hs 351316(TM4SF1), Accession No. Hs 351474(PAQR4), Accession No. Hs 351680, Accession No. Hs 351875(COX6C), Accession No. Hs 352341(STCH), Accession No. Hs 352656(GHITM), Accession No. Hs 352768(PSMB1), Accession No. Hs 354056(POR), Accession No. Hs 355141(TNIP1), Accession No. Hs 355606(MGC23909), Accession No. Hs 355643(RNPS1), Accession No. Hs 355708(FLJ20507), Accession No. Hs 355750(MGC5306), Accession No. Hs 355753(DKFZp586M1819), Accession No. Hs 355867(MARS), Accession No. Hs 355927(VDAC2), Accession No. Hs 355934(SFPQ), Accession No. Hs 355983(BZW1), Accession No. Hs 356061(MAP1LC3B), Accession No. Hs 356096(FLJ10350), Accession No. Hs 356190(UBB), Accession No. Hs 356270(SDHD), Accession No. Hs 356285(HMGN1), Accession No. Hs 356331(PPIA), Accession No. Hs 356366(RPS2), Accession No. Hs 356371(RPL28), Accession No. Hs 356377(LOC149603), Accession No. Hs 356467(MGC2747), Accession No. Hs 356501(PHF6), Accession No. Hs 356502(RPLP1), Accession No. Hs 356549(SNRPD3), Accession No. Hs 356630(NUTF2), Accession No. Hs 356647(SNX6), Accession No. Hs 356654(PSMC1), Accession No. Hs 56766(), Accession No. Hs 356769(MAN2B1), Accession No. Hs 356799, Accession No. Hs 357901(SOX4), Accession No. Hs 362728(SEP15), Accession No. Hs 365116(U2AF1), Accession No. Hs 368084(LRPPRC), Accession No. Hs 368149(CCT7), Accession No. Hs 368157(PYGB), Accession No. Hs 368240(DYRK1A), Accession No. Hs 68264(PPP2R5C), Accession No. Hs 368376(SRPR), Accession No. Hs 368402(LOC51337), Accession No. Hs 368404(EXT2), Accession No. Hs 368525(PDLIM1), Accession No. Hs 368598(LEREPO4), Accession No. Hs 368934(MGC40157), Accession No. Hs 368985(TRIP12), Accession No. Hs 369017(RAB2), Accession No. Hs 369052(SELT), Accession No. Hs 369068(DNCLI2), Accession No. Hs 369125(PSMD14), Accession No. Hs 369285(DKFZP434B168), Accession No. Hs 369356(MLL5), Accession No. Hs 369606(CPSF6), Accession No. Hs 369607(GAK), Accession No. Hs 369614(COPS2), Accession No. Hs 369615(FLJ20551), Accession No. Hs 369761(DAZAP2), Accession No. Hs 369785(MGC2749), Accession No. Hs 369920(RAP1B), Accession No. Hs 370024(SEC31L1), Accession No. Hs 370247(APLP2), Accession No. Hs 370292(BCCIP), Accession No. Hs 370312(FNTA), Accession No. Hs 370408(COMT), Accession No. Hs 370581(CAP1), Accession No. Hs 370770(XPO1), Accession No. Hs 370771(CDKN1A), Accession No. Hs 370895(RPN2), Accession No. Hs 370927(PRO1855), Accession No. Hs 370937(TAPBP), Accession No. Hs 371001(EIF3S9), Accession No. Hs 371416(CARM1), Accession No. Hs 371563(RAB14), Accession No. Hs 371788(DKFZP547E1010), Accession No. Hs 371889(ATP1A1), Accession No. Hs 372003(C9orf10), Accession No. Hs 372050(SMAP-5), Accession No. Hs 372286(CUL3), Accession No. Hs 372331(SPTAN1), Accession No. Hs 372541(KBTBD2), Accession No. Hs 372616(ARL1), Accession No. Hs 372914(NDRG1), Accession No. Hs 373550(TGIF), Accession No. Hs 373741(HM13), Accession No. Hs 373763(HNRPR), Accession No. Hs 373952(CAMTA2), Accession No. Hs 373959(VGLL4), Accession No. Hs 374043(ASXL1), Accession No. Hs 374257(SIAT4A), Accession No. Hs 374378(CKS1B), Accession No. Hs 374477(EWSR1), Accession No. Hs 374503(MORF4L1), Accession No. Hs 374588(RPL17), Accession No. Hs 374596(TPT1), Accession No. Hs 374650(IFITM3), Accession No. Hs 374973(PRPF4), Accession No. Hs 375001(TLN1), Accession No. Hs 375108(CD24), Accession No. Hs 375217(RNF31), Accession No. Hs 376046(BTN3A2), Accession No. Hs 376933(GUK1), Accession No. Hs 377155(LYRIC), Accession No. Hs 378103(RPS5), Accession No. Hs 378532(HBS1L), Accession No. Hs 378808(eIF2A), Accession No. Hs 380403(PCGF4), Accession No. Hs 380774(DDX3X), Accession No. Hs 380953(RPL38), Accession No. Hs 380973(SUMO2), Accession No. Hs 381008(HLA-E), Accession No. Hs 381058(KIAA0146), Accession No. Hs 381072(PPIF), Accession No. Hs 381123(RPL21), Accession No. Hs 381126(RPS14), Accession No. Hs 381189(CBX3), Accession No. Hs 381219, Accession No. Hs 381256(GLTP), Accession No. Hs 382044(MRPS2), Accession No. Hs 382168(NCOA3), Accession No. Hs 385913(ANP32E), Accession No. Hs 385986(UBE2B), Accession No. Hs 386434(ANXA7), Accession No. Hs 386465(CHERP), Accession No. Hs 386939(USP7), Accession No. Hs 387208(FAU), Accession No. Hs 387804(PABPC1), Accession No. Hs 388034(RXRB), Accession No. Hs 388654(ATP6V1G1), Accession No. Hs 388664(RPL11), Accession No. Hs 388739(XRCC5), Accession No. Hs 388927(YY1), Accession No. Hs 388956(C19orf22), Accession No. Hs 389037(MCM3APAS), Accession No. Hs 389107(ATP6V0C), Accession No. Hs 389171(PINK1), Accession No. Hs 389649(DDX48), Accession No. Hs 389734(TCEAL8), Accession No. Hs 389996(CHCHD2), Accession No. Hs 390667(GSTK1), Accession No. Hs 393201(ACTR2), Accession No. Hs 395482(PTK2), Accession No. Hs 396644(PAIP2), Accession No. Hs 396740(NIP30), Accession No. Hs 396783(SLC9A3R1), Accession No. Hs 397609(RPS16), Accession No. Hs 399800(AKAP8L), Accession No. Hs 400295(RPL30), Accession No. Hs 401509(RBM10), Accession No. Hs 401903(COX5A), Accession No. Hs 401929(RPL10), Accession No. Hs 403917(STK24), Accession No. Hs 404056(EIF3S1), Accession No. Hs 404321(GARS), Accession No. Hs 405144(SFRS3), Accession No. Hs 405410(OGT), Accession No. Hs 405514(LOC284058), Accession No. Hs 405590(EIF3S6), Accession No. Hs 405880(MRPS21), Accession No. Hs 405942(LOC339229), Accession No. Hs 406062(NDUFA11), Accession No. Hs 406068(UBE2M), Accession No. Hs 406096(ZA20D2), Accession No. Hs 406277(SF3A1), Accession No. Hs 406300(RPL23), Accession No. Hs 406423(SF3B2), Accession No. Hs 406510(ATP5B), Accession No. Hs 406520(LOC389541), Accession No. Hs 406534(HMG20B), Accession No. Hs 406590(PGR1), Accession No. Hs 406620(RPS10), Accession No. Hs 406683(RPS15), Accession No. Hs 406799(RAB18), Accession No. Hs 406840(SLC35A4), Accession No. Hs 407368(C19orf13), Accession No. Hs 407580(PKP4), Accession No. Hs 407995(MIF), Accession No. Hs 408018(RPL36), Accession No. Hs 408073(RPS6), Accession No. Hs 408236(TXNL5), Accession No. Hs 408257(NDUFS6), Accession No. Hs 408293(KAB), Accession No. Hs 408324(FLJ10769), Accession No. Hs 408428(CHES1), Accession No. Hs 408581(SVIL), Accession No. Hs 408909(GOLPH3), Accession No. Hs 409140(ATP5O), Accession No. Hs 409223(SSR4), Accession No. Hs 409230(AGPAT1), Accession No. Hs 409834(PHPT1), Accession No. Hs 410197(IDH3G), Accession No. Hs 410596(HAN11), Accession No. Hs 410817(RPL13), Accession No. Hs 411480(AUP1), Accession No. Hs 411641(EIF4EBP1), Accession No. Hs 411847(MAPK6), Accession No. Hs 412103(EFHA1), Accession No. Hs 412117(ANXA6), Accession No. Hs 412196(ESRRBL1), Accession No. Hs 412433(AIP), Accession No. Hs 412468(KLHDC3), Accession No. Hs 412842(C10orf7), Accession No. Hs 413036(WBSCR22), Accession No. Hs 413482(C21orf33), Accession No. Hs 414579(SCOTIN), Accession No. Hs 415342(KIAA1049), Accession No. Hs 416049(TNPO2), Accession No. Hs 416436(TRIM50A), Accession No. Hs 417004(S100A11), Accession No. Hs 417029(DERP6), Accession No. Hs 418123(CTSL), Accession No. Hs 418175(VPS28), Accession No. Hs 418233(MRPL24), Accession No. Hs 418450(MRPL11), Accession No. Hs 418533(BUB3), Accession No. Hs 418668(ATP5D), Accession No. Hs 419640(PARK7), Accession No. Hs 420269(COL6A2), Accession No. Hs 420272(H2AFY), Accession No. Hs 421257(RPL7), Accession No. Hs 421509(CCT4), Accession No. Hs 422113(ZNF511), Accession No. Hs 423935(RDBP), Accession No. Hs 423968(TTC11), Accession No. Hs 424126(SERF2), Accession No. Hs 424908(LSM5), Accession No. Hs 425777(UBE2L6), Accession No. Hs 426296(C10orf104), Accession No. Hs 426359(DKFZp564J157), Accession No. Hs 429052(ITGB1), Accession No. Hs 429353(SEPN1), Accession No. Hs 429581(RTN4), Accession No. Hs 429819(PITPNA), Accession No. Hs 429839(MGC23908), Accession No. Hs 430425(GNB1), Accession No. Hs 430551(IQGAP1), Accession No. Hs 430606(CS), Accession No. Hs 430657(ARF5), Accession No. Hs 430733(CLNS1A), Accession No. Hs 431101(GNG12), Accession No. Hs 431367(C6orf55), Accession No. Hs 431498(FOXP1), Accession No. Hs 431550(MAP4K4), Accession No. Hs 431668(COX6B1), Accession No. Hs 431850(MAPK1), Accession No. Hs 431861(PPP5C), Accession No. Hs 431926 NFKB1), Accession No. Hs 432121(PRDX2), Accession No. Hs 432438(EML4), Accession No. Hs 432491(ESD), Accession No. Hs 432690(SLC39A9), Accession No. Hs 432760(CAPZB), Accession No. Hs 432898(RPL4), Accession No. Hs 432976(NR1H2), Accession No. Hs 433154(PLSCR3), Accession No. Hs 433201(CDK2AP1), Accession No. Hs 433222(NPC2), Accession No. Hs 433291(ARD1), Accession No. Hs 433307(BCKDHA), Accession No. Hs 433343(SRRM2), Accession No. Hs 433345, Accession No. Hs 433419(COX4I1), Accession No. Hs 433512(ACTR3), Accession No. Hs 433529(RPS11), Accession No. Hs 433540(DNAJC8), Accession No. Hs 433573(Bles03), Accession No. Hs 433615(TUBB2), Accession No. Hs 433701(RPL37A), Accession No. Hs 433722(KIAA1967), Accession No. Hs 33732(CLK1), Accession No. Hs 433750(EIF4G1), Accession No. Hs 433759(BANF1), Accession No. Hs 433795(SHC1), Accession No. Hs 433863(PBP), Accession No. Hs 433901(COX8A), Accession No. Hs 433951(GPX4), Accession No. Hs 434102(HMGB1), Accession No. Hs 434207(HARS2), Accession No. Hs 434219(ANKHD1), Accession No. Hs 434401(ZNF638), Accession No. Hs 434937(PPIB), Accession No. Hs 434953(HMGB2), Accession No. Hs 434980(APP), Accession No. Hs 435044(TBC1D22A), Accession No. Hs 435064(KIAA1608), Accession No. Hs 435120(KIF1C), Accession No. Hs 435136(TXN), Accession No. Hs 435166(LBR), Accession No. Hs 435231(ZFR), Accession No. Hs 435255(UBXD1), Accession No. Hs 435326(ACTL6A), Accession No. Hs 435512(PPP3CA), Accession No. Hs 435535(ZNF395), Accession No. Hs 435610(WAC), Accession No. Hs 435741(GCSH), Accession No. Hs 435759(THAP4), Accession No. Hs 435771(API5), Accession No. Hs 435841(TNRC15), Accession No. Hs 435850(LYPLA1), Accession No. Hs 435933(PHF10), Accession No. Hs 435948(ATAD1), Accession No. Hs 435952(CDK5RAP1), Accession No. Hs 435974(MTHFD1), Accession No. Hs 436035(TUBA6), Accession No. Hs 436093(BAT2), Accession No. Hs 436204(ZNF289), Accession No. Hs 436298(EMP1), Accession No. Hs 436405(IDH3B), Accession No. Hs 436437(ALDH2), Accession No. Hs 436446(ARMET), Accession No. Hs 436500(DBNL), Accession No. Hs 436568(CD74), Accession No. Hs 436578(POLR2F), Accession No. Hs 436657(CLU), Accession No. Hs 436687(SET), Accession No. Hs 436803(VBP1), Accession No. Hs 437056(SUPT5H), Accession No. Hs 437060(CYCS), Accession No. Hs 437110(ANXA2), Accession No. Hs 437178(ACADVL), Accession No. Hs 437256(GRINL1A), Accession No. Hs 437277(MGAT4B), Accession No. Hs 437367(GBAS), Accession No. Hs 437388(PIGT), Accession No. Hs 437403(PP), Accession No. Hs 437594(RPLP2), Accession No. Hs 437638(XBP1), Accession No. Hs 437779(C11orf10), Accession No. Hs 437831(C14orf32), Accession No. Hs 438072(UNC84A), Accession No. Hs 438219(GPS2), Accession No. Hs 438429(RPS19), Accession No. Hs 438678(TALDO1), Accession No. Hs 438720(MCM7), Accession No. Hs 438970(TBL1XR1), Accession No. Hs 438974(CUTL1), Accession No. Hs 439480(RBM5), Accession No. Hs 439481(SUPT4H1), Accession No. Hs 439548(FLJ22875), Accession No. Hs 439552, Accession No. Hs 439815(HBXIP), Accession No. Hs 440382(RFP), Accession No. Hs 440544(CLIC4), Accession No. Hs 440599(DDX1), Accession No. Hs 440604(PSMD7), Accession No. Hs 440899(TTYH3), Accession No. Hs 440932(SEPT9), Accession No. Hs 440960(RAD23A), Accession No. Hs 440961(CAST), Accession No. Hs 441072(POLR2L), Accession No. Hs 441550(C20orf22), Accession No. Hs 442344(IRS2), Accession No. Hs 442798(RNF10), Accession No. Hs 443134(GBA2), Accession No. Hs 443379(PSMD11), Accession No. Hs 443837(NPEPPS), Accession No. Hs 443914(SOD1), Accession No. Hs 444279(DKFZp761C169), Accession No. Hs 444356(GRB2), Accession No. Hs 444468(CTDSP1), Accession No. Hs 444472(SDHC), Accession No. Hs 444569(VMP1), Accession No. Hs 444673(CRR9), Accession No. Hs 444724(AZI2), Accession No. Hs 444818(CGGBP1), Accession No. Hs 444931(CRSP6), Accession No. Hs 444969(C2orf4), Accession No. Hs 444986(METAP2), Accession No. Hs 445081(NS5ATP13TP2), Accession No. Hs 445351(LGALS1), Accession No. Hs 445394(VPS29), Accession No. Hs 445498(SKIIP), Accession No. Hs 445511(RIOK3), Accession No. Hs 445570(CD63), Accession No. Hs 445803(DC2), Accession No. Hs 445893(KHDRBS1), Accession No. Hs 445977(GTF3A), Accession No. Hs 446017(WSB1), Accession No. Hs 446091(WTAP), Accession No. Hs 446123(CAPZA2), Accession No. Hs 446149(LDHB), Accession No. Hs 446260(PSMA6), Accession No. Hs 446336(PXN), Accession No. Hs 446345(FTH1), Accession No. Hs 446414(CD47), Accession No. Hs 446427(OAZ1), Accession No. Hs 446445(YIF1), Accession No. Hs 446450(ITM2B), Accession No. Hs 446574(TMSB10), Accession No. Hs 446588(RPS13), Accession No. Hs 446623(HNRPL), Accession No. Hs 446628(RPS4X), Accession No. Hs 446641(ARAF), Accession No. Hs 446852(EIF3S6IP), Accession No. Hs 447477(ST13), Accession No. Hs 447492(PGAM1), Accession No. Hs 447547(VPS35), Accession No. Hs 48226(RPLP0), Accession No. Hs 448588(NGFRAP1), Accession No. Hs 448646(RPL27A), Accession No. Hs 448879, Accession No. Hs 449114(HNRPC), Accession No. Hs 449171(HNRPK), Accession No. Hs 454534(USF2), Accession No. Hs 454699(IL6ST), Accession No. Hs 456507(PKD1-like), Accession No. Hs 456557(FLJ10597), Accession No. Hs 458320(DC12), Accession No. Hs 458358(TSPYL1), Accession No. Hs 458414(IFITM1), Accession No. Hs 458458(C19orf27), Accession No. Hs 458747(ANP32A), Accession No. Hs 459106(OAZIN), Accession No. Hs 459149(BTBD1), Accession No. Hs 459174(FLJ23790), Accession No. Hs 459211(AKAP13), Accession No. Hs 459596(MPG), Accession No. Hs 459649(CLCN7), Accession No. Hs 459927(PTMA), Accession No. Hs 459940(LITAF), Accession No. Hs 460238(SH3GLB2), Accession No. Hs 460317(ALS4), Accession No. Hs 460336(GGA2), Accession No. Hs 460468(XPO6), Accession No. Hs 460499(ATXN2L), Accession No. Hs 460574(LOC124446), Accession No. Hs 460923(CNOT1), Accession No. Hs 460929(GOT2), Accession No. Hs 460978(APPBP1), Accession No. Hs 461047(G6PD), Accession No. Hs 461131(CYB5-M), Accession No. Hs 461361(CFDP1), Accession No. Hs 461379(GABARAPL2), Accession No. Hs 461722(HSPC176), Accession No. Hs 461777(PCOLN3), Accession No. Hs 461896(CRK), Accession No. Hs 461925(RPA1), Accession No. Hs 462035(UBE2G1), Accession No. Hs 462086(RIP), Accession No. Hs 462306(UBE2S), Accession No. Hs 462316(TTC19), Accession No. Hs 462492(USP22), Accession No. Hs 462550(PIGS), Accession No. Hs 462956(PPARBP), Accession No. Hs 462998(IGFBP4), Accession No. Hs 463010(SMARCE1), Accession No. Hs 463035(FKBP10), Accession No. Hs 463041(RERE), Accession No. Hs 463059(STAT3), Accession No. Hs 463295(CDC27), Accession No. Hs 463506(AKAP1), Accession No. Hs 463702(BCAS3), Accession No. Hs 463797(C1orf33), Accession No. Hs 464071(PGD), Accession No. Hs 464137(ACOX1), Accession No. Hs 464210(SYNGR2), Accession No. Hs 464336(P4HB), Accession No. Hs 464438(AGTRAP), Accession No. Hs 464472(MRLC2), Accession No. Hs 464595(PPP4R1), Accession No. Hs 464652(TNFSF5IP1), Accession No. Hs 464912(P15RS), Accession No. Hs 465224(NARS), Accession No. Hs 465374(EFHD2), Accession No. Hs 465498(TXNL4A), Accession No. Hs 465529(MIDN), Accession No. Hs 465543(BTBD2), Accession No. Hs 465627(MAP2K2), Accession No. Hs 465645(C19orf10), Accession No. Hs 465808(HNRPM), Accession No. Hs 465849(PIN1), Accession No. Hs 465924(SDHB), Accession No. Hs 466044(PKN1), Accession No. Hs 466088(TPM4), Accession No. Hs 466148(NR2F6), Accession No. Hs 466471(GPI), Accession No. Hs 466693(SIRT2), Accession No. Hs 466766(LTBP4), Accession No. Hs 466775(SNRPA), Accession No. Hs 467084(EIF4G3), Accession No. Hs 467097(SNRP70), Accession No. Hs 467192(PPP2R1A), Accession No. Hs 467279(LENG4), Accession No. Hs 467284(RPS9), Accession No. Hs 467408(TRIM28), Accession No. Hs 467637(CDC42), Accession No. Hs 467696(HPCAL1), Accession No. Hs 467701(ODC1), Accession No. Hs 467807(LAPTM4A), Accession No. Hs 467824(PUM2), Accession No. Hs 467960(RAB10), Accession No. Hs 468018(PPP1CB), Accession No. Hs 468415(PIGF), Accession No. Hs 468442(CALM2), Accession No. Hs 468760(AFTIPHILIN), Accession No. Hs 469022(DGUOK), Accession No. Hs 469171(DKFZP564D0478), Accession No. Hs 469331(STARD7), Accession No. Hs 469820(RALB), Accession No. Hs 469863(YWHAZ), Accession No. Hs 469925(FLJ14346), Accession No. Hs 469970(SFRS4), Accession No. Hs 470091(YWHAE), Accession No. Hs 470233(ARL5), Accession No. Hs 470417, Accession No. Hs 470477(PTP4A2), Accession No. Hs 470577(EIF2S2), Accession No. Hs 470588(KPNA6), Accession No. Hs 470943(STAT1), Accession No. Hs 471011(SF3B1), Accession No. Hs 471104(NOP5/NOP58), Accession No. Hs 471207(NDUFS1), Accession No. s 471441(PSMB2), Accession No. Hs 471461(ACSL3), Accession No. Hs 471593(CAB39), Accession No. Hs 471768(MGC4796), Accession No. Hs 471818(M11S1), Accession No. Hs 471851(HDLBP), Accession No. Hs 471873(DTYMK), Accession No. Hs 471933(FKBP1A), Accession No. Hs 471975(C20orf116), Accession No. Hs 472010(PRNP), Accession No. Hs 472024(C20orf30), Accession No. Hs 472031(UBE2D3), Accession No. Hs 472038(CGI-94), Accession No. Hs 472056(SYNCRIP), Accession No. Hs 472119(MKKS), Accession No. Hs 472185(NDUFS5), Accession No. Hs 472213(RRBP1), Accession No. Hs 472330(C20orf3), Accession No. Hs 472475(MACF1), Accession No. Hs 472535(AKIP), Accession No. Hs 472558(SDBCAG84), Accession No. Hs 472651(BLCAP), Accession No. Hs 472737(TOP1), Accession No. Hs 473296(TPD52L2), Accession No. Hs 473583(NSEP1), Accession No. Hs 473648(GART), Accession No. Hs 473721(SLC2A1), Accession No. Hs 473761(RTN3), Accession No. Hs 473788(OTUB1), Accession No. Hs 474005(SUMO3), Accession No. Hs 474010(PTTG1IP), Accession No. Hs 474053(COL6A1), Accession No. Hs 474083(B4GALT2), Accession No. Hs 474213(UFD1L), Accession No. Hs 474584(AKR1A1), Accession No. Hs 474643(HSPC117), Accession No. Hs 474751(MYH9), Accession No. Hs 474833(CSNK1E), Accession No. Hs 474914(RUTBC3), Accession No. Hs 474938(SLC25A17), Accession No. Hs 474949(RBX1), Accession No. Hs 474982(ACO2), Accession No. Hs 475125(ATXN10), Accession No. Hs 475319(LRRFIP2), Accession No. Hs 475382(FLJ22405), Accession No. Hs 475392(LOC55831), Accession No. Hs 475663(RAB5A), Accession No. Hs 475733(TOP2B), Accession No. Hs 475812(SIMP), Accession No. Hs 476018(CTNNB1), Accession No. Hs 476033(TLP19), Accession No. Hs 476179(SMARCC1), Accession No. Hs 476221(IHPK2), Accession No. Hs 76231(IMPDH2), Accession No. Hs 476308(ALAS1), Accession No. Hs 476365(SCP2), Accession No. Hs 476448(FLNB), Accession No. Hs 476706(MRPL37), Accession No. Hs 476930(DKFZP564O123), Accession No. Hs 477157(DULLARD), Accession No. Hs 477789(ATP1B3), Accession No. Hs 477892(GYG), Accession No. Hs 478000(MBNL1), Accession No. Hs 478044(PA2G4), Accession No. Hs 478553(EIF4A2), Accession No. Hs 479208(FBXL5), Accession No. Hs 479264(LAP3), Accession No. Hs 479634(SLC30A9), Accession No. Hs 479693(SFRS11), Accession No. Hs 479728(GAPD), Accession No. Hs 479747(BCAR1), Accession No. Hs 479814(POLR2B), Accession No. Hs 480073(HNRPD), Accession No. Hs 480311(PDLIM5), Accession No. Hs 480465(SCYE1), Accession No. Hs 80653(ANXA5), Accession No. Hs 481571(UQCRH), Accession No. Hs 481720(MYO10), Accession No. Hs 481898(KAT3), Accession No. Hs 482144(RPL26), Accession No. Hs 482363(SLC30A5), Accession No. Hs 482526(TINP1), Accession No. Hs 482868(KIAA0372), Accession No. Hs 483036(PJA2), Accession No. Hs 483067(C5orf13), Accession No. Hs 483305(HINT1), Accession No. Hs 483408(PPP2CA), Accession No. Hs 483454(CNN3), Accession No. Hs 483486(JMJD1B), Accession No. Hs 484138(FBXW11), Accession No. Hs 484188(ATP6V0E), Accession No. 484242(ETEA), Accession No. Hs 484288(DDX41), Accession No. Hs 484363(RNF130), Accession No. Hs 484551(CPM), Accession No. Hs 484813(DEK), Accession No. Hs 485155(RPL35), Accession No. Hs 485195(SORT1), Accession No. Hs 485246(PSMA5), Accession No. Hs 485262(MTCH1), Accession No. Hs 485365(AHCYL1), Accession No. Hs 485616(DST), Accession No. Hs 486542(BCLAF1), Accession No. Hs 487027(VIL2), Accession No. Hs 487054(TCP1), Accession No. Hs 487635(BZW2), Accession No. Hs 487774(HNRPA2B1), Accession No. Hs 488171(KIAA1068), Accession No. Hs 488181(OGDH), Accession No. Hs 488307(DKFZP564K0822), Accession No. Hs 488478(FLJ10099), Accession No. Hs 488671(BAZ1B), Accession No. Hs 489207(ASNS), Accession No. Hs 489284(ARPC1B), Accession No. Hs 489287(CPSF4), Accession No. Hs 489336(SYAP1), Accession No. Hs 489615(PBEF1), Accession No. Hs 490203(CALD1), Accession No. Hs 490394(SSBP1), Accession No. Hs 490415(ZYX), Accession No. Hs 490745(DNAJB6), Accession No. Hs 490795(CHR2SYT), Accession No. Hs 490874(MTX1), Accession No. Hs 491336(ELP3), Accession No. Hs 491359(LMNA), Accession No. Hs 491440(PPP2CB), Accession No. Hs 491494(CCT3), Accession No. Hs 491597(VDAC3), Accession No. Hs 491695(UBE2V2), Accession No. Hs 491745(TCEA1), Accession No. Hs 491988(TRAM1), Accession No. Hs 492236(WDR42A), Accession No. Hs 492314(LAPTM4B), Accession No. Hs 492445(EDD), Accession No. Hs 492599(EIF3S3), Accession No. Hs 492805(CGI-07), Accession No. Hs 493362(AK3L1), Accession No. Hs 493750(WDR40A), Accession No. Hs 494173(ANXA1), Accession No. Hs 494419(LAMP1), Accession No. Hs 494457(NINJ1), Accession No. Hs 494604(ANP32B), Accession No. Hs 494614(XTP2), Accession No. Hs 494691(PFN1), Accession No. Hs 494700(CDW92), Accession No. Hs 494985(FBXW2), Accession No. Hs 495039(NDUFA8), Accession No. Hs 495349(KIAA0515), Accession No. Hs 495471(PMPCA), Accession No. Hs 495605(CD99), Accession No. Hs 495851(MGC4825), Accession No. Hs 495960(ATP6AP2), Accession No. Hs 496068(PCTK1), Accession No. Hs 496098(DKFZp761A052), Accession No. Hs 496271, Accession No. Hs 496487(ATF4), Accession No. Hs 496646(IL13RA1), Accession No. Hs 496684(LAMP2), Accession No. Hs 497183(IVNS1ABP), Accession No. Hs 497599(WARS), Accession No. Hs 497692(C1orf48), Accession No. Hs 497893(ENAH), Accession No. Hs 498239(FH), Accession No. Hs 498313(ADSS), Accession No. Hs 498317(PNAS-4), Accession No. Hs 498455(KIAA0217), Accession No. Hs 498548(RBM17), Accession No. Hs 498727(DHCR24), Accession No. Hs 499145(YME1L1), Accession No. Hs 499158(GGA1), Accession No. Hs 499594(TIMM23), Accession No. Hs 499833(C10orf74), Accession No. Hs 499891(HNRPH3), Accession No. Hs 499925(VPS26), Accession No. Hs 499960(SARA1), Accession No. Hs 500067(PPP3CB), Accession No. Hs 500101(VCL), Accession No. Hs 500375(ENTPD6), Accession No. Hs 500409(GLUD1), Accession No. Hs 500546(IDE), Accession No. Hs 500674(SMBP), Accession No. Hs 500775(ZNF207), Accession No. Hs 500842(MGEA5), Accession No. Hs 500874(CUEDC2), Accession No. Hs 501012(ADD3), Accession No. Hs 501023(MXI1), Accession No. Hs 501203(TIAL1), Accession No. Hs 501293(BSG), Accession No. Hs 501309(CIRBP), Accession No. Hs 501353(PLEKHJ1), Accession No. Hs 501376(UROS), Accession No. Hs 501420(NCLN), Accession No. Hs 501629(IER2), Accession No. Hs 501684(NAP1L4), Accession No. Hs 501735(STIM1), Accession No. Hs 501853(C11orf15), Accession No. Hs 501924(USP47), Accession No. Hs 501991(MLSTD2), Accession No. Hs 502302(CAT), Accession No. Hs 502328(CD44), Accession No. Hs 502461(DGKZ), Accession No. Hs 502528(NDUFS3), Accession No. Hs 502630(C11orf31), Accession No. Hs 502659(RHOC), Accession No. Hs 502705(PRP19), Accession No. Hs 502745(FADS2), Accession No. Hs 502769(SLC3A2), Accession No. Hs 502773(MTCBP-1), Accession No. Hs 502823(PRDX5), Accession No. Hs 502829(SF1), Accession No. Hs 502836(ARL2), Accession No. Hs 502842(CAPN1), Accession No. Hs 502872(MAP3K11), Accession No. Hs 502876(RHOB), Accession No. Hs 503093(ZFP36L2), Accession No. Hs 503222(RAB6A), Accession No. Hs 503251(PME-1), Accession No. Hs 503597(HSPC148), Accession No. Hs 503709(PORIMIN), Accession No. Hs 503716(MGC2714), Accession No. Hs 503787(DARS), Accession No. Hs 504237(ITM1), Accession No. Hs 504517(RPS27), Accession No. Hs 504613(PTMS), Accession No. Hs 504620(REA), Accession No. Hs 504687(MYL9), Accession No. Hs 504828(DDX47), Accession No. Hs 504895(STRAP), Accession No. Hs 505033(KRAS2), Accession No. Hs 505059(PSMD4), Accession No. Hs 505625(C12orf10), Accession No. Hs 505652(COPZ1), Accession No. Hs 505676(CIP29), Accession No. Hs 505705(MYL6), Accession No. Hs 505806(PBXIP1), Accession No. Hs 505824(CGI-51), Accession No. Hs 506215(RARS), Accession No. Hs 506325(NUDT4), Accession No. Hs 06759(ATP2A2), Accession No. Hs 506861(DDX54), Accession No. Hs 507074(KIAA0152), Accession No. Hs 507162(FLJ12750), Accession No. Hs 507584(MGC9850), Accession No. Hs 507680(PFAAP5), Accession No. Hs 507910(PGRMC2), Accession No. Hs 507916(TGFB1I4), Accession No. Hs 508010(FNDC3A), Accession No. Hs 508644(FLJ10154), Accession No. Hs 509163(KIAA1181), Accession No. Hs 509226(FKBP3), Accession No. Hs 509264(KLHDC2), Accession No. Hs 509414(KTN1), Accession No. Hs 509622(RGL2), Accession No. Hs 509736(HSPCB), Accession No. Hs 509791(ERH), Accession No. Hs 509909(NUMB), Accession No. Hs 510087(ENSA), Accession No. Hs 510328(DDX24), Accession No. Hs 510402(MCP), Accession No. Hs 511067(FLJ10579), Accession No. Hs 511138(DKFZP564G2022), Accession No. Hs 511149(SNAP23), Accession No. Hs 511425(SRP9), Accession No. Hs 511504(TCF12), Accession No. Hs 511862, Accession No. Hs 511952(CBX6), Accession No. Hs 512005(ARPC3), Accession No. Hs 512465(SURF4), Accession No. Hs 512525(RPS17), Accession No. Hs 512607(MIR16), Accession No. Hs 512640(PRKCSH), Accession No. Hs 512661(KIAA1160), Accession No. Hs 512676, Accession No. Hs 512693(FLJ20859), Accession No. Hs 512756(THAP7), Accession No. Hs 512815(AP3D1), Accession No. Hs 512857(CD151), Accession No. Hs 512867(H63), Accession No. Hs 512908(ARPP-19), Accession No. Hs 513043(C15orf12), Accession No. Hs 513055(REC14), Accession No. Hs 513057(RANBP5), Accession No. Hs 513058(TMED3), Accession No. Hs 513071(MESDC1), Accession No. Hs 513083(RPL9), Accession No. Hs 513141(IDH2), Accession No. Hs 513145(NEUGRIN), Accession No. Hs 513153(FURIN), Accession No. Hs 513230(MRPL28), Accession No. Hs 513242(RHOT2), Accession No. Hs 513261(C16orf34), Accession No. Hs 513266(NDUFB10), Accession No. Hs 513470(NFATC2IP), Accession No. Hs 513488(MVP), Accession No. Hs 513490(ALDOA), Accession No. Hs 513520(BCKDK), Accession No. Hs 513522(FUS), Accession No. Hs 513631(ARL2BP), Accession No. Hs 513856(DPH2L1), Accession No. Hs 513984(FLII), Accession No. Hs 514012(MAP2K3), Accession No. Hs 514036(SDF2), Accession No. Hs 514038(FLOT2), Accession No. Hs 514174(JUP), Accession No. Hs 514196(RPL27), Accession No. Hs 514211(MGC4251), Accession No. Hs 514216(CGI-69), Accession No. Hs 514220(GRN), Accession No. Hs 514297(FLJ13855), Accession No. Hs 514303(PHB), Accession No. Hs 514435(SF3B3), Accession No. Hs 514489(WBP2), Accession No. Hs 514535(LGALS3BP), Accession No. Hs 514581(ACTG1), Accession No. Hs 514590(HGS), Accession No. Hs 514819(AP2B1), Accession No. Hs 514870(ATP5F1), Accession No. Hs 514920(NDP52), Accession No. Hs 514934(CAPZA1), Accession No. Hs 515003(C19orf6), Accession No. Hs 515005(STK11), Accession No. Hs 515018(GNA13), Accession No. Hs 515053(AES), Accession No. Hs 515070(EEF2), Accession No. Hs 515092(CLPP), Accession No. Hs 515155(MGC2803), Accession No. Hs 515162(CALR), Accession No. Hs 515164(GADD45GIP1), Accession No. Hs 515210(DNAJB1), Accession No. Hs 515255(LSM4), Accession No. Hs 515266(RENT1), Accession No. Hs 515271(SFRS14), Accession No. Hs 515329(RPL22), Accession No. Hs 515371(CAPNS1), Accession No. Hs 515406(AKT2), Accession No. Hs 515417(EGLN2), Accession No. Hs 515432(DEDD2), Accession No. Hs 515472(SNRPD2), Accession No. Hs 515475(SYMPK), Accession No. Hs 515487(CALM3), Accession No. Hs 515494(SLC1A5), Accession No. Hs 515500(SAE1), Accession No. Hs 515515(KDELR1), Accession No. Hs 515517(RPL18), Accession No. Hs 515524(NUCB1), Accession No. Hs 515540(PTOV1), Accession No. Hs 515550(LOC284361), Accession No. Hs 515598(PRPF31), Accession No. Hs 515607(PPP1R12C), Accession No. Hs 515642(GPSN2), Accession No. Hs 515785(BLVRB), Accession No. Hs 515846(RUVBL2), Accession No. Hs 515848(HADHB), Accession No. Hs 515890(YPEL5), Accession No. Hs 516075(TIA1), Accession No. Hs 516077(FLJ14668), Accession No. Hs 516087(TEX261), Accession No. Hs 516111(DCTN1), Accession No. Hs 516114(WBP1), Accession No. Hs 516157(MAT2A), Accession No. Hs 516450(FLJ20297), Accession No. Hs 516522(FLJ21919), Accession No. Hs 516539(HNRPA3), Accession No. Hs 516587(UBE2Q), Accession No. Hs 516633(NCKAP1), Accession No. Hs 516711(CHPF), Accession No. Hs 516790(ARHGEF2), Accession No. Hs 516807(STK25), Accession No. Hs 516826(TRIB3), Accession No. Hs 516855(CENPB), Accession No. Hs 517080(SLC35C2), Accession No. Hs 517106(CEBPB), Accession No. Hs 517134(C20orf43), Accession No. Hs 517145(ENO1), Accession No. Hs 517168(TAGLN2), Accession No. Hs 517216(PEA15), Accession No. Hs 517232(PEX19), Accession No. Hs 517240(IFNGR2), Accession No. Hs 517262(SON), Accession No. Hs 517293(F11R), Accession No. Hs 517338(ATP6V1E1), Accession No. Hs 17342(DEDD), Accession No. Hs 517356(COL18A1), Accession No. Hs 517357(DGCR2), Accession No. Hs 517421(PCQAP), Accession No. Hs 517438(ASCC2), Accession No. Hs 517517(EP300), Accession No. Hs 517543(PES1), Accession No. Hs 517582(MCM5), Accession No. Hs 517622(UNC84B), Accession No. Hs 517641(L3MBTL2), Accession No. Hs 517666(DIA1), Accession No. Hs 517731(PP2447), Accession No. Hs 517768(DKFZP564B167), Accession No. Hs 517792(C3orf10), Accession No. Hs 517817(MGC3222), Accession No. Hs 517821, Accession No. Hs 517888(CRTAP), Accession No. Hs 517948(DHX30), Accession No. Hs 517949(MAP4), Accession No. Hs 517969(APEH), Accession No. Hs 517981(TUSC2), Accession No. Hs 518060(ARL6IP5), Accession No. Hs 518123(TFG), Accession No. Hs 518236(SEC61A1), Accession No. Hs 518244(RPN1), Accession No. Hs 518249(ZNF9), Accession No. Hs 518250(COPG), Accession No. Hs 518265(H41), Accession No. Hs 518326(SERP1), Accession No. Hs 518346(SSR3), Accession No. Hs 518374(QSCN6), Accession No. Hs 518424(NDUFB5), Accession No. Hs 518460(AP2M1), Accession No. Hs 518464(PSMD2), Accession No. Hs 518525(GLUL), Accession No. Hs 518551(RPL31), Accession No. Hs 518608(PP784), Accession No. Hs 518609(ARPC5), Accession No. Hs 518750(OCIAD1), Accession No. Hs 18805(HMGA1), Accession No. Hs 518827(CCNI), Accession No. Hs 519276(MAPKAPK2), Accession No. Hs 519304(PELO), Accession No. Hs 519346(ERBB2IP), Accession No. Hs 519347(SFRS12), Accession No. Hs 519520(RPS25), Accession No. Hs 519523(SERPINB6), Accession No. Hs 519557(TMEM14C), Accession No. Hs 519718(TTC1), Accession No. Hs 519756(STK10), Accession No. Hs 519818(MGAT1), Accession No. Hs 519909(MARCKS), Accession No. Hs 519930(C6orf62), Accession No. Hs 520026(VARS2), Accession No. Hs 520028(HSPA1A), Accession No. Hs 520037(NEU1), Accession No. Hs 520070(C6orf82), Accession No. Hs 520140(SRF), Accession No. Hs 520189(ELOVL5), Accession No. Hs 520205(EIF2AK1), Accession No. Hs 520210(KDELR2), Accession No. Hs 520287(C6orf111), Accession No. Hs 520313(CD164), Accession No. Hs 520383(STX7), Accession No. Hs 520421(PERP), Accession No. Hs 520459(GTF2I), Accession No. Hs 520623(C7orf27), Accession No. Hs 520640(ACTB), Accession No. Hs 520740(SCRN1), Accession No. Hs 520794(YKT6), Accession No. Hs 520898(CTSB), Accession No. Hs 520943(WBSCR1), Accession No. Hs 520967(MDH2), Accession No. Hs 520973(HSPB1), Accession No. Hs 520974(YWHAG), Accession No. Hs 521064(ZNF655), Accession No. Hs 521151(FLJ22301), Accession No. Hs 521289(REPIN1), Accession No. Hs 521487(MGC8721), Accession No. Hs 521640(RAD23B), Accession No. Hs 521809(COBRA1), Accession No. Hs 521903(LY6E), Accession No. Hs 521924(SIAHBP1), Accession No. Hs 521969(NDUFB11), Accession No. Hs 521973(WDR13), Accession No. Hs 522074(DSIPI), Accession No. Hs 522110(CREB3), Accession No. Hs 522114(CLTA), Accession No. Hs 522310(NANS), Accession No. Hs 522373(GSN), Accession No. Hs 522394(HSPA5), Accession No. Hs 522463(EEF1A1), Accession No. Hs 522507(FBXW5), Accession No. Hs 522584(TMSB4X), Accession No. Hs 522590(EIF1AX), Accession No. Hs 522632(TIMP1), Accession No. Hs 522665(MAGED2), Accession No. Hs 522675(FLJ12525), Accession No. Hs 522752(PSMD10), Accession No. Hs 522817(BCAP31), Accession No. Hs 522819(IRAK1), Accession No. Hs 522823(EMD), Accession No. Hs 522932(NCOA4), Accession No. Hs 522995(EIF4EBP2), Accession No. Hs 523004(PSAP), Accession No. Hs 523012(DDIT4), Accession No. Hs 523054(SMP1), Accession No. Hs 523131(TRAPPC3), Accession No. Hs 523145(DDOST), Accession No. Hs 523215(NDUFB8), Accession No. Hs 523238(NOLC1), Accession No. Hs 523262(C1orf8), Accession No. Hs 523299(EIF3S10), Accession No. Hs 523302(PRDX3), Accession No. Hs 523560(HSPCA), Accession No. Hs 523680(SSRP1), Accession No. Hs 523789(TncRNA), Accession No. Hs 523829(POLD4), Accession No. Hs 523836(GSTP1), Accession No. Hs 523852(CCND1), Accession No. Hs 523875(INPPL1), Accession No. Hs 524009(AASDHPPT), Accession No. Hs 524081(DPAGT1), Accession No. Hs 524084(RNF26), Accession No. Hs 524161(RSU1), Accession No. Hs 524171(RAD52), Accession No. Hs 524183(FKBP4), Accession No. Hs 524195(ARHGAP21), Accession No. Hs 524214(MLF2), Accession No. Hs 524219(TPI1), Accession No. Hs 524271(PHC2), Accession No. Hs 524367(CBARA1), Accession No. Hs 524395(TUBA3), Accession No. Hs 524464(ATP5G2), Accession No. Hs 524502(RNF41), Accession No. Hs 524530(CTDSP2), Accession No. Hs 524590(RAB21), Accession No. Hs 524599(NAP1L1), Accession No. Hs 524690(PPIE), Accession No. Hs 524788(RAB35), Accession No. Hs 524809(RSN), Accession No. Hs 524899(SAP18), Accession No. Hs 524920(ZFP91), Accession No. Hs 524969(Ufm1), Accession No. Hs 525134(FLJ20277), Accession No. Hs 525163(ANKRD10), Accession No. Hs 525232(LRP10), Accession No. Hs 525238(C14orf119), Accession No. Hs 525330(ARF6), Accession No. Hs 525391(FLJ20580), Accession No. Hs 525527(RER1), Accession No. Hs 525626(PACS1L), Accession No. Hs 525899(C6orf49), Accession No. Hs 526464(PML), Accession No. Hs 526521(MDH1), Accession No. Hs 527105(HNRPDL), Accession No. Hs 527193(RPS23), Accession No. Hs 527348(AKAP9), Accession No. Hs 527412(ASAH1), Accession No. Hs 527861(OS-9), Accession No. Hs 527862(PKD1), Accession No. Hs 527980(DUT), Accession No. Hs 528050(HARS), Accession No. Hs 528222(NDUFS4), Accession No. Hs 528300(PITRM1), Accession No. Hs 528305(DDX17), Accession No. Hs 528572(SCAM-1), Accession No. Hs 528668(RPL6), Accession No. Hs 528780(GSPT1), Accession No. Hs 528803(UQCRC2), Accession No. Hs 529059(EIF3S4), Accession No. Hs 529132(SEPW1), Accession No. Hs 529244(NCK2), Accession No. Hs 529280(ANAPC7), Accession No. Hs 529303(ARPC2), Accession No. Hs 529369(AFAP), Accession No. Hs 529400(IFNAR1), Accession No. Hs 529420(UBE2G2), Accession No. Hs 529591(TLOC1), Accession No. Hs 529618(TFRC), Accession No. Hs 529631(RPL35A), Accession No. Hs 529782(VCP), Accession No. Hs 529798(BTF3), Accession No. Hs 529862(CSNK1A1), Accession No. Hs 529890(CANX), Accession No. Hs 529892(SQSTM1), Accession No. Hs 529957(SEC63), Accession No. Hs 530096(EIF3S2), Accession No. Hs 530118(LUC7L2), Accession No. Hs 530291(ANXA11), Accession No. Hs 530314(SSNA1), Accession No. Hs 530331(PDHA1), Accession No. Hs 530381(PIM3), Accession No. Hs 530412(PAI-RBP1), Accession No. Hs 530436(STXBP3), Accession No. Hs 530479(PMF1), Accession No. Hs 530687(RNH), Accession No. Hs 530734(MRPL16), Accession No. Hs 530753(FLJ20625), Accession No. Hs 530823(COPS7A), Accession No. Hs 530862(PRKAG1), Accession No. Hs 531081(LGALS3), Accession No. Hs 531089(PSMA3), Accession No. Hs 531176(SARS), Accession No. Hs 531330(CBWD1), Accession No. Hs 531614(BTBD14B), Accession No. Hs 531752(RANBP3), Accession No. Hs 531856(GAS5), Accession No. Hs 531876(DNCL2A), Accession No. Hs 531879(RAD1), Accession No. Hs 532359(RPL5), Accession No. Hs 532399(KIAA0663), Accession No. Hs 532755(GTL3), Accession No. Hs 532790(NMT1), Accession No. Hs 532793(KPNB1), Accession No. Hs 532803(HN1), Accession No. Hs 532826(MCL1), Accession No. Hs 532853(NDUFB7), Accession No. Hs 533030(HRIHFB2122), Accession No. Hs 533059(TUBB), Accession No. Hs 533122(SFRS10), Accession No. Hs 533136(LRPAP1), Accession No. Hs 533192(TOMM20), Accession No. Hs 533222(HSA9761), Accession No. Hs 533245(DDX46), Accession No. Hs 533282(NONO), Accession No. Hs 533308(PPP2R5D), Accession No. Hs 533317(VIM), Accession No. Hs 533437(TCEB1), Accession No. Hs 533440(WWP1), Accession No. Hs 533474(PPP1R8), Accession No. Hs 533479(LYPLA2), Accession No. Hs 533526(ATRX), Accession No. Hs 533624(H3F3A), Accession No. Hs 533712(RBM4), Accession No. Hs 533732(SRP14), Accession No. Hs 533771(STUB1), Accession No. Hs 533782(KRT8), Accession No. Hs 533977(TXNIP), Accession No. Hs 533985(EXOC7), Accession No. Hs 533986(ZNF258), Accession No. Hs 534125(HLA-C), Accession No. Hs 534168(NDUFA1), Accession No. Hs 534212(SEC22L1), Accession No. Hs 534255(B2M), Accession No. Hs 534307(CCND3), Accession No. Hs 534314(EIF5A), Accession No. Hs 534326(ITGB4BP), Accession No. Hs 534338(PPP4C), Accession No. Hs 534346(RPS7), Accession No. Hs 534350(SMARCB1), Accession No. Hs 534453(GRIM19), Accession No. Hs 534456(ANAPC11), Accession No. Hs 534457(C14orf166), Accession No. Hs 534473(TOMM22), Accession No. Hs 534483(MGC2941), Accession No. Hs 536275(PACS1), Accession No. Hs 541269(NDUFB9), Accession No. Hs 546248(CTSD), Accession No. Hs 546250(DNCI2), Accession No. Hs 546253(FDFT1), Accession No. Hs 546261(HNRPA1), Accession No. Hs 546269(RPL10A), Accession No. Hs 546271(PCBP2), Accession No. Hs 546286(RPS3), Accession No. Hs 546289(RPS12), Accession No. Hs 546290(RPS18), Accession No. Hs 546291(NET-5), Accession No. Hs 546339(SMAP), Accession No. Hs 546356(RPL13A), Accession No. Hs 546394(HSPC016), Accession No. Hs 547759(SSBP3), Accession No. Hs 549178(C9orf86), Accession No. Hs 552590(HTF9C), Accession No. Hs 553496(PGM3), Accession No. Hs 553512(C3F), Accession No. Hs 554767(NUP88), Accession No. Hs 554776(SREBF1), Accession No. Hs 554894(FLJ12953), Accession No. Hs 554896(MGC11257), Accession No. Hs 555194(FAM36A), Accession No. Hs 555866(C1QBP), Accession No. Hs 555873(HNRPAB), Accession No. Hs 555875(IDH3A), Accession No. Hs 555889(PSMC2), Accession No. Hs 555890(RBBP4), Accession No. Hs 555911(RBM8A), Accession No. Hs 555969(RIC8), Accession No. Hs 555971(PP1201), Accession No. Hs 555973(MRPS25), Accession No. Hs 555994(LONP), Accession No. Hs 556267(FBXL10), Accession No. Hs 556461(NDUFV2), Accession No. Hs 556795(PAICS), Accession No. Hs 557550(NPM1), Accession No. Hs 558296(ACP1), Accession No. Hs 558313(COX6A1), Accession No. Hs 558322(EEF1B2), Accession No. Hs 558325(EIF5), Accession No. Hs 558328(FKBP5), Accession No. Hs 558330(FTL), Accession No. Hs 558338(HSPE1), Accession No. Hs 558345(IK), Accession No. Hs 558354(LAMR1), Accession No. Hs 558360(NDUFB4), Accession No. Hs 558361(NME2), Accession No. Hs 558362(NUMA1), Accession No. Hs 558376(RAC1), Accession No. Hs 558381(RNU65), Accession No. Hs 558382(RPL15), Accession No. Hs 558383(RPL18A), Accession No. Hs 558384(RPL19), Accession No. Hs 558385(RPL23A), Accession No. Hs 558386(RPL34), Accession No. Hs 558388(RPS3A), Accession No. Hs 558389(RPS8), Accession No. Hs 558390(RPS24), Accession No. Hs 558391(RPS26), Accession No. Hs 558396(SCD), Accession No. Hs 558424(CSDA), Accession No. Hs 558426(EIF3S5), Accession No. Hs 558429(G10), Accession No. Hs 558431(RPL14), Accession No. Hs 558442(PDCD6IP), Accession No. Hs 558448(TXNL2), Accession No. Hs 558453(ATP5L), Accession No. Hs 558454(NUDC), Accession No. Hs 558458(COPS8), Accession No. Hs 558473(C18orf10), Accession No. Hs 558499(8D6A), Accession No. Hs 558511(PSARL), Accession No. Hs 558521(C2orf33), Accession No. Hs 558591(ORMDL1), Accession No. Hs 558825(PDE4DIP), Accession No. Hs 558995, Accession No. Hs 567260(CD2BP2), Accession No. Hs 567263(C1orf43), Accession No. Hs 567267(ATP2C1) 및 Accession No. Hs 567279(HCNGP) 로 이루어진 군으로부터 선택된 하나 또는 둘 이상의 유전자 인 것을 특징으로 한다. 또한, 상기 검출 시약은 상기 후보 표준 발현 유전자를 특이적으로 증폭할 수 있는 프라이머쌍 및/또는 프로브인 것을 특징으로 한다.The selected candidate standard expression gene was Accession No. Hs 120 (PRDX6), Accession No. Hs 142 (SULT1A1), Accession No. Hs 202 (BZRP), Accession No. Hs 429 (ATP5G3), Accession No. Hs 695 (CSTB), Accession No. Hs 808 (HNRPF), Accession No. Hs 861 (MAPK3), Accession No. Hs 1063 (SNRPC), Accession No. Hs 1103 (TGFB1), Accession No. Hs 2430 (TCFL1), Accession No. Hs 2533 (ALDH9A1), Accession No. Hs 2795 (LDHA), Accession No. Hs 2853 (PCBP1), Accession No. Hs 3100 (KARS), Accession No. Hs 3254 (MRPL23), Accession No. Hs 3353 (G3BP), Accession No. Hs 3416 (ADFP), Accession No. Hs 439 (STOML2), Accession No. Hs 3530 (FUSIP1), Accession No. Hs 3989 (PLXNB2), Accession No. Hs 4055 (KLF6), Accession No. Hs 4742 (GPAA1), Accession No. Hs 4747 (DKC1), Accession No. Hs 4766 (FAM32A), Accession No. Hs 4859 (CCNL1), Accession No. Hs 4997 (RBM23), Accession No. Hs 4998 (TMOD3), Accession No. Hs 5062 (TM4SF8), Accession No. Hs 5086 (MGC10433), Accession No. Hs 5120 (DNCL1), Accession No. Hs 5158 (ILK), Accession No. Hs 5245 (FLJ20643), Accession No. Hs 5258 (MAGED1), Accession No. Hs 5268 (ZDHHC4), Accession No. Hs 5298 (ADIPOR1), Accession No. Hs 5308 (UBA52), Accession No. Hs 5324 (C2orf25), Accession No. Hs 5345 (RNPEPL1), Accession No. Hs 5662 (GNB2L1), Accession No. Hs 5710 (CREG1), Accession No. Hs 5719 (CNAP1), Accession No. Hs 5912 (FBXO7), Accession No. Hs 5947 (RAB8A), Accession No. Hs 6396 (JTB), Accession No. Hs 6454 (RGS19IP1), Accession No. Hs 6459 (GPR172A), Accession No. Hs 6551 (ATP6AP1), Accession No. Hs 6891 (SFRS6), Accession No. Hs 7101 (ANAPC5), Accession No. Hs 7236 (NOSIP), Accession No. Hs 7476 (ATP6V0B), Accession No. Hs 7527 (DKFZP566E144), Accession No. Hs 7744 (NDUFV1), Accession No. Hs 7753 (CALU), Accession No. Hs 7768 (FIBP), Accession No. Hs 7862 (PNRC2), Accession No. Hs 7910 (RYBP), Accession No. Hs 7917 (HIG1), Accession No. Hs 8102 (RPS20), Accession No. Hs 8372 (UQCR), Accession No. Hs 8737 (WDR6), Accession No. Hs 8752 (TMEM4), Accession No. Hs 8765 (DDX42), Accession No. Hs 8859 (CANT1), Accession No. Hs 8867 (CYR61), Accession No. Hs 9003 (FLJ13868), Accession No. Hs 9015 (MGC52000), Accession No. Hs 9043 (C14orf120), Accession No. Hs 9234 (NIFIE14), Accession No. Hs 9235 (NME4), Accession No. Hs 9527 (C2orf28), Accession No. Hs 9534 (SEC11L1), Accession No. Hs 9573 (ABCF1), Accession No. Hs 9589 (UBQLN1), Accession No. Hs 9788 (NDFIP1), Accession No. Hs 9825 (CGI-128), Accession No. Hs 9857 (DCXR), Accession No. Hs 10326 (COPE), Accession No. Hs 10842 (RAN), Accession No. Hs 10848 (BMS1L), Accession No. Hs 11125 (SPCS1), Accession No. Hs 11184 (UBE2R2), Accession No. Hs 11223 (IDH1), Accession No. Hs 11355 (TMPO), Accession No. Hs 11463 (UMP-CMPK), Accession No. Hs 12013 (ABCE1), Accession No. Hs 12084 (TUFM), Accession No. Hs 12102 (SNX3), Accession No. Hs 12107 (BC-2), Accession No. Hs 12109 (WDR39), Accession No. Hs 12144 (KIAA1033), Accession No. Hs 12152 (SRPRB), Accession No. Hs 12272 (BECN1), Accession No. Hs 12341 (ADAR), Accession No. Hs 12457 (NUP133), Accession No. Hs 12865 (NSFL1C), Accession No. Hs 13662 (MGC5508), Accession No. Hs 14317 (NOLA3), Accession No. Hs 14333 (FLJ10349), Accession No. Hs 14745 (C10orf9), Accession No. Hs 14839 (POLR2G), Accession No. Hs 14846 (SLC7A1), Accession No. Hs 14894 (TGOLN2), Accession No. Hs 15277 (C16orf33), Accession No. Hs 15591 (COPS6), Accession No. Hs 15738 (RAB7), Accession No. Hs 16059 (HSPC009), Accession No. Hs 16130 (E2-230K), Accession No. Hs 16349 (KIAA0431), Accession No. Hs 17118 (FLJ11730), Accession No. Hs 17250 (MGC4767), Accession No. Hs 17680 (FUCA2), Accession No. Hs 17731 (FLJ12892), Accession No. Hs 17883 (PPM1G), Accession No. Hs 18069 (LGMN), Accession No. Hs 18128 (C20orf44), Accession No. Hs 18349 (MRPL15), Accession No. Hs 19673 (MAF1), Accession No. Hs 20013 (P29), Accession No. Hs 20107 (KNS2), Accession No. Hs 20157 (CDK5RAP3), Accession No. Hs 20521 (HRMT1L2), Accession No. Hs 20529 (LOC127262), Accession No. Hs 20573 (IGF1R), Accession No. Hs 20716 (TIMM17A), Accession No. Hs 22393 (DENR), Accession No. Hs 22543 (UBE3A), Accession No. Hs 22546 (CYBASC3), Accession No. Hs 22616 (KIAA0664), Accession No. Hs 23033 (LOC92912), Accession No. Hs 23111 (FARSLA), Accession No. Hs 23978 (SAFB), Accession No. Hs 24301 (POLR2E), Accession No. Hs 24379 (TRAPPC1), Accession No. Hs 24601 (FBLN1), Accession No. Hs 24950 (RGS5), Accession No. Hs 25155 (NET1), Accession No. Hs 25450 (SLC29A1), Accession No. Hs 25723 (MTVR1), Accession No. Hs 26010 (PFKP), Accession No. Hs 26023 (FOXJ3), Accession No. Hs 26136 (MGC14156), Accession No. Hs 26232 (MAN2C1), Accession No. Hs 26403 (GSTZ1), Accession No. Hs 26518 (TM4SF7), Accession No. Hs 27222 (NOLA2), Accession No. Hs 28491 (SAT), Accession No. Hs 28914 (APRT), Accession No. Hs 29203 (GBL), Accession No. Hs 29665 (CLSTN1), Accession No. Hs 30011 (MGC2963), Accession No. Hs 30026 (HSPC182), Accession No. Hs 30345 (TRAP1), Accession No. Hs 30954 (PMVK), Accession No. Hs 31053 (CKAP1), Accession No. Hs 31334 (C20orf14), Accession No. Hs 31387 (DKFZP564J0123), Accession No. Hs 34045 (CDCA4), Accession No. Hs 34576 (TAX1BP1), Accession No. Hs 34906 (BLOC1S2), Accession No. Hs 35052 (TEGT), Accession No. Hs 35828 (MARK3), Accession No. Hs 36587 (PPP1R7), Accession No. Hs 36927 (HSPH1), Accession No. Hs 37616 (STRA13), Accession No. Hs 37916 (DPP7), Accession No. Hs 42806 (Cab45), Accession No. Hs 43297 (MTPN), Accession No. Hs 47062 (POLR2I), Accession No. Hs 50098 (NDUFA4), Accession No. Hs 50308 (HIP2), Accession No. Hs 50425 (TEBP), Accession No. Hs 53066 (HSPBP1), Accession No. Hs 54277 (FAM50A), Accession No. Hs 54457 (CD81), Accession No. Hs 54642 (MAT2B), Accession No. Hs 54649 (RY1), Accession No. Hs 55682 (EIF3S7), Accession No. Hs 55847 (MRPL51), Accession No. Hs 58488 (CTNNAL1), Accession No. Hs 58992 (SMC4L1), Accession No. Hs 59486 (HSDL2), Accession No. Hs 61812 (PTPN12), Accession No. Hs 65234 (DDX27), Accession No. Hs 65238 (RNF40), Accession No. Hs 66048 (BPY2IP1), Accession No. Hs 66915 (C22orf16), Accession No. Hs 68714 (SFRS1), Accession No. Hs 9293 (HEXB), Accession No. Hs 69554 (RNF126), Accession No. Hs 69855 (UNR), Accession No. Hs 71465 (SQLE), Accession No. Hs 71787 (MRPS7), Accession No. Hs 73527 (CSNK2B), Accession No. Hs 73722 (APEX1), Accession No. Hs 73799 (GNAI3), Accession No. Hs 73965 (SFRS2), Accession No. Hs 74047 (ETFB), Accession No. Hs 74050 (FVT1), Accession No. Hs 74137 (TMP21), Accession No. Hs 74375 (DVL1), Accession No. Hs 74405 (YWHAQ), Accession No. Hs 74471 (GJA1), Accession No. Hs 74563 (OAZ2), Accession No. Hs 74564 (SSR2), Accession No. Hs 74576 (GDI1), Accession No. Hs 75056 (TIMM13), Accession No. Hs 75061 (MARCKSL1), Accession No. Hs 75066 (TSN), Accession No. Hs 75087 (FASTK), Accession No. Hs 75117 (ILF2), Accession No. Hs 75133 (TFAM), Accession No. Hs 75139 (ARFIP2), Accession No. Hs 75189 (DAP), Accession No. Hs 75227 (NDUFA9), Accession No. Hs 75243 (BRD2), Accession No. Hs 75249 (ARL6IP), Accession No. Hs 75254 (IRF3), Accession No. Hs 75318 (TUBA1), Accession No. Hs 75348 (PSME1), Accession No. Hs 75438 (QDPR), Accession No. Hs 75527 (ADSL), Accession No. Hs 75724 (COPB2), Accession No. Hs 75798 (C20orf111), Accession No. Hs 75841 (C12orf8), Accession No. Hs 75890 (MBTPS1), Accession No. Hs 75914 (RNP24), Accession No. Hs 76111 (DAG1), Accession No. Hs 76394 (ECHS1), Accession No. Hs 76480 (UBL4), Accession No. Hs 76662 (ZDHHC16), Accession No. Hs 76686 (GPX1), Accession No. Hs 76847 (GANAB), Accession No. Hs 77060 (PSMB6), Accession No. Hs 77269 (GNAI2), Accession No. Hs 77313 (CDK10), Accession No. Hs 77422 (PLP2), Accession No. Hs 77558 (HMGN3), Accession No. Hs 77578 (USP9X), Accession No. Hs 77793 (CSK), Accession No. Hs 77897 (SF3A3), Accession No. Hs 77961 (HLA-B), Accession No. Hs 77978 (DKFZp761I2123), Accession No. Hs 78466 (PSMD8), Accession No. Hs 78601 (UROD), Accession No. Hs 78771 (PGK1), Accession No. Hs 78880 (ILVBL), Accession No. Hs 78888 (DBI), Accession No. Hs 78989 (ADH5), Accession No. Hs 79064 (DHPS), Accession No. Hs 79081 (PPP1CC), Accession No. Hs 79088 (RCN2), Accession No. Hs 79101 (CCNG1), Accession No. Hs 79110 (NCL), Accession No. Hs 79322 (QARS), Accession No. Hs 79335 (SMARCD1), Accession No. Hs 79387 (PSMC5), Accession No. Hs 79402 (POLR2C), Accession No. Hs 79411 (RPA2), Accession No. Hs 79625 (C20orf149), Accession No. Hs 80545 (RPL37), Accession No. Hs 80919 (SYPL), Accession No. Hs 80986 (ATP5G1), Accession No. Hs 81328 (NFKBIA), Accession No. Hs 81424 (SUMO1), Accession No. Hs 81848 (RAD21), Accession No. Hs 81964 (SEC24C), Accession No. Hs 82201 (CSNK2A2), Accession No. Hs 82327 (GSS), Accession No. Hs 82719 (MGC21416), Accession No. Hs 82793 (PSMB3), Accession No. Hs 82887 (PPP1R11), Accession No. Hs 82890 (DAD1), Accession No. Hs 82916 (CCT6A), Accession No. Hs 82927 (AMPD2), Accession No. Hs 83190 (FASN), Accession No. Hs 83347 (AAMP), Accession No. Hs 83383 (PRDX4), Accession No. Hs 83734 (STX4A), Accession No. Hs 83753 (SNRPB), Accession No. Hs 83765 (DHFR), Accession No. Hs 83916 (NDUFA5), Accession No. Hs 84359 (GABARAP), Accession No. Hs 84753 (FLJ12442), Accession No. Hs 85155 (ZFP36L1), Accession No. Hs 85769 (ERBP), Accession No. Hs 85962 (DERPC), Accession No. Hs 86131 (FADD), Accession No. Hs 87752 (MSN), Accession No. Hs 89545 (PSMB4), Accession No. Hs 89643 (TKT), Accession No. Hs 89649 (EPHX1), Accession No. Hs 89781 (UBTF), Accession No. Hs 89864 (SKIV2L), Accession No. Hs 90061 (PGRMC1), Accession No. Hs 90093 (HSPA4), Accession No. Hs 90107 (ADRM1), Accession No. Hs 90443 (NDUFS8), Accession No. Hs 91142 (KHSRP), Accession No. Hs 91531 (MLLT6), Accession No. Hs 93659 (ERP70), Accession No. Hs 93832 (LOC54499), Accession No. Hs 95577 (CDK4), Accession No. Hs 96530 (COX11), Accession No. Hs 96852 (FLJ21128), Accession No. Hs 96996 (HNRPA0), Accession No. Hs 97616 (SH3GL1), Accession No. Hs 97887 (RCN1), Accession No. Hs 98751 (FUBP3), Accession No. Hs 98791 (ACTR1B), Accession No. Hs 102696 (MCTS1), Accession No. Hs 102798 (PSMA1), Accession No. Hs 103561 (ARL6IP4), Accession No. Hs 103834 (MGC5576), Accession No. Hs 104839 (TIMP2), Accession No. Hs 105547 (NPDC1), Accession No. Hs 106185 (RALGDS), Accession No. Hs 106876 (ATP6V0D1), Accession No. Hs 106909 (ANAPC13), Accession No. Hs 107003 (CCNB1IP1), Accession No. Hs 107101 (FLJ31031), Accession No. Hs 107387 (C7orf20), Accession No. Hs 107393 (C3orf4), Accession No. Hs 108029 (SH3BGRL), Accession No. Hs 108080 (CSRP1), Accession No. Hs 108371 (E2F4), Accession No. Hs 108408 (APH-1A), Accession No. Hs 108957 (RPS27L), Accession No. Hs 108969 (PTD008), Accession No. Hs 109051 (SH3BGRL3), Accession No. Hs 109052 (C14orf2), Accession No. Hs 109672 (SIAT7F), Accession No. Hs 109798 (C6orf48), Accession No. Hs 110695 (SF3B5), Accession No. Hs 110849 (ESRRA), Accession No. Hs 111286 (MRPS11), Accession No. Hs 111577 (ITM2C), Accession No. Hs 111801 (ARS2), Accession No. Hs 112058 (SIVA), Accession No. Hs 112318 (TOMM7), Accession No. Hs 112955 (NUDT5), Accession No. Hs 114033 (SSR1), Accession No. Hs 114286 (CD9), Accession No. Hs 114412 (TXNL1), Accession No. Hs 115474 (RFC3), Accession No. Hs 115792 (EXOSC7), Accession No. Hs 116448 (GLS), Accession No. Hs 117176 (PABPN1), Accession No. Hs 117715 (ST5), Accession No. Hs 118110 (BST2), Accession No. Hs 118400 (FSCN1), Accession No. Hs 118463 (PNPLA2), Accession No. Hs 118638 (NME1), Accession No. Hs 118722 (FUT8), Accession No. Hs 118964 (p66alpha), Accession No. Hs 118983 (GSDMDC1), Accession No. Hs 19177 (ARF3), Accession No. Hs 119192 (H2AFZ), Accession No. Hs 119251 (UQCRC1), Accession No. Hs 119591 (AP2S1), Accession No. Hs 119598 (RPL3), Accession No. Hs 120323 (DNAPTP6), Accession No. Hs 121088 (NUP153), Accession No. Hs 121549 (CDIPT), Accession No. Hs 122363 (WIPI-2), Accession No. Hs 122523 (SND1), Accession No. Hs 124126 (ARPC1A), Accession No. Hs 124147 (FBXL11), Accession No. Hs 124246 (C10orf119), Accession No. Hs 124366 (BBX), Accession No. Hs 125113 (CCT8), Accession No. Hs 125867 (EVL), Accession No. Hs 125898 (GNAS), Accession No. Hs 126497 (AEBP2), Accession No. Hs 126774 (RAMP), Accession No. Hs 126938 (NAPA), Accession No. Hs 127092 (DHX38), Accession No. Hs 127249 (EAP30), Accession No. Hs 127386 (MAMDC2), Accession No. Hs 127764 (RAB5C), Accession No. Hs 128065 (CTSC), Accession No. Hs 128199 (SEPT11), Accession No. Hs 128548 (WDR1), Accession No. Hs 129634 (CINP), Accession No. Hs 129673 (EIF4A1), Accession No. Hs 130031 (TRIO), Accession No. Hs 130098 (DDX23), Accession No. Hs 130293 (CROP), Accession No. Hs 130413 (TM9SF2), Accession No. Hs 131226 (BNIP3L), Accession No. Hs 132497 (PRNPIP), Accession No. Hs 132513 HSD17B12), Accession No. Hs 133892 (TPM1), Accession No. Hs 134074 (SLC35E1), Accession No. Hs 134688 (PSMD13), Accession No. Hs 135406 (CEBPZ), Accession No. Hs 136905 (UREB1), Accession No. Hs 136947 (RALY), Accession No. Hs 137510 (NCOR2), Accession No. Hs 138860 (ARHGAP1), Accession No. Hs 139896 (MAEA), Accession No. Hs 140452 (M6PRBP1), Accession No. Hs 142442 (HP1-BP74), Accession No. Hs 143187 (DDX49), Accession No. Hs 143766 (DRPLA), Accession No. Hs 143873 (S100A10), Accession No. Hs 144058 (EBSP), Accession No. Hs 144468 (MGC3234), Accession No. Hs 144835 (EEF1G), Accession No. Hs 144868 (VTI1B), Accession No. Hs 144941 (MUF1), Accession No. Hs 144949 (ZNF313), Accession No. Hs 144980 (SCAMP4), Accession No. Hs 145049 (PLEKHM2), Accession No. Hs 145442 (MAP2K1), Accession No. Hs 145575 (UBL3), Accession No. Hs 146070 (TPM3), Accession No. Hs 146393 (HERPUD1), Accession No. Hs 146602 (QP-C), Accession No. Hs 146804 (SPIN), Accession No. Hs 146806 (CUL1), Accession No. Hs 147433 (PCNA), Accession No. Hs 148078 (RBAF600), Accession No. Hs 148272 (CCM2), Accession No. Hs 148330 (ARF4), Accession No. Hs 148340 (PTPRG), Accession No. Hs 148670 (RHOBTB1), Accession No. Hs 149004 (FBXO31), Accession No. Hs 149957 (RPS6KA1), Accession No. Hs 149983 (PEX14), Accession No. Hs 150107 (BIRC6), Accession No. Hs 150540 (BC002942), Accession No. Hs 150580 (SUI1), Accession No. Hs 150837 (TXNDC5), Accession No. Hs 151134 (OXA1L), Accession No. Hs 151220 (KIAA0992), Accession No. Hs 151413 (GMFB), Accession No. Hs 151787 (U5-116KD), Accession No. Hs 152536 (p44S10), Accession No. Hs 153177 (RPS28), Accession No. Hs 154023 (TXNDC4), Accession No. Hs 154073 (SLC35B1), Accession No. Hs 155165 (ZFPL1), Accession No. Hs 155218 (HNRPUL1), Accession No. Hs 155396 (NFE2L2), Accession No. Hs 155829 (KIAA0676), Accession No. Hs 156171 (PSMC6), Accession No. Hs 156367 (RPS29), Accession No. Hs 156667 (KIAA1536), Accession No. Hs 157160 (MRPS34), Accession No. Hs 157351 (PTD004), Accession No. Hs 157379 (H2AFV), Accession No. Hs 157394 (HAGH), Accession No. Hs 159014 (PRPF4B), Accession No. Hs 159118 (AMD1), Accession No. Hs 159130 (RAF1), Accession No. Hs 159161 (ARHGDIA), Accession No. Hs 159699 (FBXO21), Accession No. Hs 159799 (THRAP2), Accession No. Hs 160958 (CDC37), Accession No. Hs 161357 (PDHB), Accession No. Hs 162032 (HBP1), Accession No. Hs 162233 (CHD4), Accession No. Hs 162877 (PACSIN2), Accession No. Hs 163645 (MOCS2), Accession No. Hs 163776 (UBE2J1), Accession No. Hs 163893 (PICALM), Accession No. Hs 165195 (VAPA), Accession No. Hs 166011 (CTNND1), Accession No. Hs 166204 (PHF1), Accession No. Hs 166463 (HNRPU), Accession No. Hs 166924 (SEC13L1), Accession No. Hs 166975 (SFRS5), Accession No. Hs 167535 (SRP54), Accession No. Hs 168073 (TRPC4AP), Accession No. Hs 168799 (METTL3), Accession No. Hs 169611 (DIABLO), Accession No. Hs 169718 (CNN2), Accession No. Hs 170107 (UQCRFS1), Accession No. Hs 170131 (NFIC), Accession No. Hs 170553 (CNOT7), Accession No. Hs 170622 (CFL1), Accession No. Hs 171626 (SKP1A), Accession No. Hs 172550 (PTBP1), Accession No. Hs 172755 (BRP44L), Accession No. Hs 172928 (COL1A1), Accession No. Hs 173024 (NYREN18), Accession No. Hs 173162 (NOC4), Accession No. Hs 173381 (DPYSL2), Accession No. Hs 173464 (FKBP8), Accession No. Hs 173611 (NDUFS2), Accession No. Hs 173705 (LOC401152), Accession No. Hs 173724 (CKB), Accession No. Hs 174050 (EDF1), Accession No. Hs 174195 (IFITM2), Accession No. Hs 175473 (AK1), Accession No. Hs 175955 (YT521), Accession No. Hs 177530 (ATP5E), Accession No. Hs 177766 (PARP1), Accession No. Hs 178551 (RPL8), Accession No. Hs 178728 (MBD3), Accession No. Hs 179986 (FLOT1), Accession No. Hs 180141 (CFL2), Accession No. Hs 180312 (MRPS16), Accession No. Hs 180414 (HSPA8), Accession No. Hs 180877 (H3F3B), Accession No. Hs 180903 (384D8-2), Accession No. Hs 180909 (PRDX1), Accession No. Hs 180933 (CXXC1), Accession No. Hs 181046 (DUSP3), Accession No. Hs 181112 (MED4), Accession No. Hs 181163 (HMGN2), Accession No. Hs 181244 (HLA-A), Accession No. Hs 181368 (PRPF8), Accession No. Hs 181444 (TMEM9), Accession No. Hs 182255 (NHP2L1), Accession No. Hs 182626 (C22orf5), Accession No. Hs 182885 (SLC35B2), Accession No. Hs 183684 (EIF4G2), Accession No. Hs 183706 (ADD1), Accession No. Hs 183800 (RANGAP1), Accession No. Hs 183850 (DCTD), Accession No. Hs 183994 (PPP1CA), Accession No. Hs 184062 (C20orf24), Accession No. Hs 184211 (PMPCB), Accession No. Hs 184233 (HSPA9B), Accession No. Hs 184492 (ELAVL1), Accession No. Hs 185172 (GNB2), Accession No. Hs 185597 (SPG7), Accession No. Hs 187199 (MALAT1), Accession No. Hs 187635 (RPS15A), Accession No. Hs 187763 (BRD4), Accession No. Hs 187866 (SDFR1), Accession No. Hs 187946 (SLC20A1), Accession No. Hs 188501 (PAFAH1B2), Accession No. Hs 188614 (PLEKHA5), Accession No. Hs 188879 (RBM6), Accession No. Hs 188882 (NUDT3), Accession No. Hs 189075 (PTK9), Accession No. Hs 189119 (CXXC5), Accession No. Hs 189329 (SMURF1), Accession No. Hs 189716 (NDUFAB1), Accession No. Hs 189772 (CCT2), Accession No. Hs 190028 (GSTO1), Accession No. Hs 190086 (MRCL3), Accession No. Hs 190334 (RAP1A), Accession No. Hs 190384 (COPS4), Accession No. Hs 190722 (HSPC142), Accession No. Hs 190904 (STRN4), Accession No. Hs 191186 (TTC17), Accession No. Hs 191346 (SEPT7), Accession No. Hs 191518 (DHX9), Accession No. Hs 191987 (UBE2J2), Accession No. Hs 192316 (CDC2L1), Accession No. Hs 192374 (TRA1), Accession No. Hs 192425 (EIF3S8), Accession No. Hs 193118 (RAI17), Accession No. Hs 193163 (BIN1), Accession No. Hs 193491 (TUBB6), Accession No. Hs 194329 (TCEAL4), Accession No. Hs 194718 (ZNF265), Accession No. Hs 195464 (FLNA), Accession No. Hs 195642 (C17orf27), Accession No. Hs 196983 (SSFA2), Accession No. Hs 198281 (PKM2), Accession No. Hs 199561 (RANBP2), Accession No. Hs 199625 (HAX1), Accession No. Hs 200063 (HDAC7A), Accession No. Hs 200600 (SCAMP3), Accession No. Hs 200804 (SDCBP), Accession No. Hs 201253 (ch-TOG), Accession No. Hs 201390 (WDR45L), Accession No. Hs 201712 (GLG1), Accession No. Hs 202011 (GK001), Accession No. Hs 202085 (VDAC1), Accession No. Hs 202166 (HNRPH1), Accession No. Hs 202179 (SMN2), Accession No. Hs 203099 (KIAA0261), Accession No. Hs 203910 (SGTA), Accession No. Hs 204041 (AHSA1), Accession No. Hs 204773 (MEP50), Accession No. Hs 205 163 (MRPL3), Accession No. Hs 206 500 (CTTN), Accession No. Hs 206824 (MGC71993), Accession No. Hs 208597 (CTBP1), Accession No. Hs 209983 (STMN1), Accession No. Hs 210469 (ELMO2), Accession No. Hs 210532 (KIAA0141), Accession No. Hs 211463 (DNM2), Accession No. Hs 211594 (PSMC4), Accession No. Hs 211914 (NDUFS7), Accession No. Hs 212102 (TXNDC7), Accession No. Hs 212395 (CIZ1), Accession No. Hs 213061 (NUCKS), Accession No. Hs 213470 (PSMB7), Accession No. Hs 213541, Accession No. Hs 213666 (KIAA0460), Accession No. Hs 213724 (SUPT16H), Accession No. Hs 216653 (FBXO9), Accession No. Hs 220950 (FOXO3A), Accession No. Hs 221847 (SLC38A2), Accession No. Hs 222510 (DAZAP1), Accession No. Hs 223141 (DDX21), Accession No. Hs 224607 (SDC1), Accession No. Hs 226007 (RDH11), Accession No. Hs 226117 (H1F0), Accession No. Hs 226755 (YWHAH), Accession No. Hs 227067 (ATAD3A), Accession No. Hs 227253 (TOMM70A), Accession No. Hs 227777 (PTP4A1), Accession No. Hs 229641 (PC4), Accession No. Hs 231295 (PITPNC1), Accession No. Hs 231616 (HSPC023), Accession No. Hs 232194 (KIAA0174), Accession No. Hs 232543 (PDCD4), Accession No. Hs 233458 (NFYC), Accession No. Hs 233552 (CDC2L5), Accession No. Hs 233952 (PSMA7), Accession No. Hs 234521 (MAPKAPK3), Accession No. Hs 236030 (SMARCC2), Accession No. Hs 237536 (MGC20781), Accession No. Hs 237971 (XTP3TPA), Accession No. Hs 238839 (SCYL1), Accession No. Hs 240170 (MGC2731), Accession No. Hs 241336 (ATPIF1), Accession No. Hs 241543 (POLDIP2), Accession No. Hs 241558 (ARIH2), Accession No. Hs 241575 (GNPTG), Accession No. Hs 241576 (DERL1), Accession No. Hs 241579 (SERPINH1), Accession No. Hs 242458 (SPG21), Accession No. Hs 242947 (DGKI), Accession No. Hs 246112 (ASCC3L1), Accession No. Hs 246310 (ATP5J), Accession No. Hs 246413 (CPNE1), Accession No. Hs 246781 (FBXO11), Accession No. Hs 247077 (RHOA), Accession No. Hs 247186 (FBS1), Accession No. Hs 247975 (HSPD1), Accession No. Hs 248267 (MPST), Accession No. Hs 248941 (TAF9), Accession No. Hs 49600 (DLGAP4), Accession No. Hs 250009 (ARL10C), Accession No. Hs 250429 (SUPT6H), Accession No. Hs 250758 (PSMC3), Accession No. Hs 250899 (HSBP1), Accession No. Hs 250905 (LOC51234), Accession No. Hs 251531 (PSMA4), Accession No. Hs 252457 (MVD), Accession No. Hs 252713 (TTC15), Accession No. Hs 252967 DKFZp566C0424), Accession No. Hs 253726 (PAPOLA), Accession No. Hs 253903 (STOM), Accession No. Hs 254042 (BAT1), Accession No. Hs 255015 (VPS24), Accession No. Hs 255093 (PFKL), Accession No. Hs 255932 (XRN2), Accession No. Hs 255935 (BTG1), Accession No. Hs 255973 (CRI1), Accession No. Hs 256301 (MGC13170), Accession No. Hs 256549 (NUBP2), Accession No. Hs 257008 (PLD3), Accession No. Hs 257341 (SAV1), Accession No. Hs 57761 (SH3BP5), Accession No. Hs 258551 (DNPEP), Accession No. Hs 258563 (FEZ2), Accession No. Hs 258798 C10orf86), Accession No. Hs 259461 (PALM2-AKAP2), Accession No. Hs 260603 (PIP5K2B), Accession No. Hs 262823 (FLJ10326), Accession No. Hs 265829 (ITGA3), Accession No. Hs 268488 (KIAA1185), Accession No. Hs 268530 (GPS1), Accession No. Hs 268742 (C13orf12), Accession No. Hs 268849 (GLO1), Accession No. Hs 268939 (MATR3), Accession No. Hs 269528 (MAK3), Accession No. Hs 269577 (PTPRA), Accession No. Hs 269782 (GNAQ), Accession No. Hs 269944 (MTCH2), Accession No. Hs 270291 (ACTN4), Accession No. Hs 270428 (SUCLG1), Accession No. Hs 270525 (LASS5), Accession No. Hs 270869 (ZNF410), Accession No. Hs 271135 (ATP5C1), Accession No. Hs 271695 (NOB1P), Accession No. Hs 272062 (PTPRF), Accession No. Hs 272168 (TDE1), Accession No. Hs 272630 (ATP6V1D), Accession No. Hs 272927 (SEC23A), Accession No. Hs 273077 (TMEM14B), Accession No. Hs 274184 (TFE3), Accession No. Hs 274772 (C15orf15), Accession No. Hs 274873 (CARS), Accession No. Hs 275243 (S100A6), Accession No. Hs 275775 (SEPP1), Accession No. Hs 275865 (PCNP), Accession No. Hs 276878 (NUP93), Accession No. Hs 277035 (MGLL), Accession No. Hs 277517 (C11orf2), Accession No. Hs 278186 (ARHGEF1), Accession No. Hs 278362 (MEA), Accession No. Hs 278426 (PDAP1), Accession No. Hs 278429 (C9orf78), Accession No. Hs 278500 (GNPDA1), Accession No. Hs 278569 (SNX17), Accession No. Hs 278573 (CD59), Accession No. Hs 278721 (SLC39A7), Accession No. Hs 279061 (C17orf25), Accession No. Hs 279245 (TACC1), Accession No. Hs 279257 (PCMT1), Accession No. Hs 279413 (POLD1), Accession No. Hs 279529 (PX19), Accession No. Hs 279583 (DREV1), Accession No. Hs 279623 (SEPX1), Accession No. Hs 279640 (TPR), Accession No. Hs 279652 (MRPL4), Accession No. Hs 279669 (TUBG1), Accession No. Hs 279696 (SUMF2), Accession No. Hs 279806 (DDX5), Accession No. Hs 79836 (COMMD9), Accession No. Hs 279920 (YWHAB), Accession No. Hs 279929 (TMED9), Accession No. Hs 80202 (SBF1), Accession No. Hs 280342 (PRKAR1A), Accession No. Hs 280378 (SNRPB2), Accession No. Hs 282410 (CALM1), Accession No. Hs 282700 (SPCS2), Accession No. Hs 282901 (RNPC2), Accession No. Hs 282998 (RBM9), Accession No. Hs 283111 (C14orf124), Accession No. Hs 283454 (BNIP2), Accession No. Hs 283521 (RHEB), Accession No. Hs 283610 (APG4B), Accession No. Hs 283652 (IDI1), Accession No. Hs 283739 (UBQLN4), Accession No. Hs 284208 (ANKRD25), Accession No. Hs 284279 (HMOX2), Accession No. Hs 284286 (MRPS24), Accession No. Hs 284491 (PDXK), Accession No. Hs 285354 (MAX), Accession No. Hs 285976 (LASS2), Accession No. Hs 286221 (ARF1), Accession No. Hs 286226 (MYO1C), Accession No. Hs 288193 (KPNA4), Accession No. Hs 288856 (PFDN5), Accession No. Hs 288969 (HSCARG), Accession No. Hs 289008 (C6orf68), Accession No. Hs 289092 (COTL1), Accession No. Hs 289123 (DCTN2), Accession No. Hs 289271 (CYC1), Accession No. Hs 290243 (GBF1), Accession No. Hs 290404 (SLC25A3), Accession No. Hs 290758 (DDB1), Accession No. Hs 291587 (ARID1B), Accession No. Hs 292026 (EIF4E2), Accession No. Hs 292063 (EIF4B), Accession No. Hs 292078 (LARP), Accession No. Hs 292265 (ZMYND11), Accession No. Hs 292457, Accession No. Hs 292493 (G22P1), Accession No. Hs 292524 (CCNH), Accession No. Hs 292579 (PTDSS1), Accession No. Hs 293563 (FLJ12666), Accession No. Hs 295917 (ATP6V1B2), Accession No. Hs 297324 (TIMP3), Accession No. Hs 298198 (CKLFSF3), Accession No. Hs 298280 (ATP5A1), Accession No. Hs 298654 (DUSP6), Accession No. Hs 299002 (FBL), Accession No. Hs 299055 (GDI2), Accession No. Hs 300141 (RPL39), Accession No. Hs 300684 (RCP9), Accession No. Hs 300772 (TPM2), Accession No. Hs 300816 RAB1B), Accession No. Hs 300834 (GALNT2), Accession No. Hs 301404 (RBM3), Accession No. Hs 301412 (Ufc1), Accession No. Hs 302742 (MRPS6), Accession No. Hs 302903 (UBE2I), Accession No. Hs 303676 (G3BP2), Accession No. Hs 304 192 (DSTN), Accession No. Hs 304682 (CST3), Accession No. Hs 306123 (MAGEF1), Accession No. Hs 306242 (RANBP9), Accession No. Hs 306329 (ZA20D3), Accession No. Hs 306425 (IBTK), Accession No. Hs 308122 (ITPK1), Accession No. Hs 308340 (NUP188), Accession No. Hs 308709 (GRP58), Accession No. Hs 309090 (SFRS7), Accession No. Hs 309231 (C6orf153), Accession No. Hs 309641 (RNF11), Accession No. Hs 309753 (STARD3NL), Accession No. Hs 309849 (C14orf159), Accession No. Hs 310542 (TOMM40), Accession No. Hs 310645 (RAB1A), Accession No. Hs 311072 (MRPS35), Accession No. Hs 311346 (CMAS), Accession No. Hs 311609 (DDX39), Accession No. Hs 311640 (RPS27A), Accession No. Hs 312098 (ADAM15), Accession No. Hs 313847 (TXNDC11), Accession No. Hs 314263 (BAZ2A), Accession No. Hs 314359 (EIF3S12), Accession No. Hs 315177 (IFRD2), Accession No. Hs 315230 (GC20), Accession No. Hs 319334 (NASP), Accession No. Hs 321391 (MGC4549), Accession No. Hs 321541 (RAB11A), Accession No. Hs 323363 (APG9L1), Accession No. Hs 323489 (FLJ20758), Accession No. Hs 324250 (NDUFB2), Accession No. Hs 324844 (VKORC1), Accession No. Hs 325650 (EHD2), Accession No. Hs 326387 (MORF4L2), Accession No. Hs 330384 (CORO1C), Accession No. Hs 331431 (SCC-112), Accession No. Hs 333388 (EEF1D), Accession No. Hs 333579 (HSPC152), Accession No. Hs 333786 (PSMA2), Accession No. Hs 333823 (MRPL13), Accession No. Hs 334017 (K-ALPHA-1), Accession No. Hs 334479 (TRAF7), Accession No. Hs 334534 (GNS), Accession No. Hs 334587 (RBPMS), Accession No. Hs 334713 (BMSC-UbP), Accession No. Hs 334851 (LASP1), Accession No. Hs 334868 (PPP2R5E), Accession No. Hs 335003 (ANKRD11), Accession No. Hs 335057 (SEPT2), Accession No. Hs 335163 (KIAA1102), Accession No. Hs 335918 (FDPS), Accession No. Hs 337295 (STIP1), Accession No. Hs 337766 (TXNRD1), Accession No. Hs 339278 (COPB), Accession No. Hs 339639 (COX7A2L), Accession No. Hs 339697 (GRINA), Accession No. Hs 343911 (EI24), Accession No. Hs 345694 (KCMF1), Accession No. Hs 346868 (EBNA1BP2), Accession No. Hs 348418 (DR1), Accession No. Hs 349656 (SCARB2), Accession No. Hs 350194 (ZMAT2), Accession No. Hs 350229 (CASC3), Accession No. Hs 350268 (IRF2BP2), Accession No. Hs 350364 (C9orf10OS), Accession No. Hs 350927 (SLC25A6), Accession No. Hs 351099 (FLJ10241), Accession No. Hs 351296 (LOC51035), Accession No. Hs 351316 (TM4SF1), Accession No. Hs 351474 (PAQR4), Accession No. Hs 351680, Accession No. Hs 351875 (COX6C), Accession No. Hs 352341 (STCH), Accession No. Hs 352656 (GHITM), Accession No. Hs 352768 (PSMB1), Accession No. Hs 354056 (POR), Accession No. Hs 355141 (TNIP1), Accession No. Hs 355606 (MGC23909), Accession No. Hs 355643 (RNPS1), Accession No. Hs 355708 (FLJ20507), Accession No. Hs 355750 (MGC5306), Accession No. Hs 355753 (DKFZp586M1819), Accession No. Hs 355867 (MARS), Accession No. Hs 355927 (VDAC2), Accession No. Hs 355934 (SFPQ), Accession No. Hs 355983 (BZW1), Accession No. Hs 356061 (MAP1LC3B), Accession No. Hs 356096 (FLJ10350), Accession No. Hs 356190 (UBB), Accession No. Hs 356270 (SDHD), Accession No. Hs 356285 (HMGN1), Accession No. Hs 356331 (PPIA), Accession No. Hs 356366 (RPS2), Accession No. Hs 356371 (RPL28), Accession No. Hs 356377 (LOC149603), Accession No. Hs 356467 (MGC2747), Accession No. Hs 356501 (PHF6), Accession No. Hs 356502 (RPLP1), Accession No. Hs 356549 (SNRPD3), Accession No. Hs 356630 (NUTF2), Accession No. Hs 356647 (SNX6), Accession No. Hs 356654 (PSMC1), Accession No. Hs 56766 (), Accession No. Hs 356769 (MAN2B1), Accession No. Hs 356799, Accession No. Hs 357901 (SOX4), Accession No. Hs 362728 (SEP15), Accession No. Hs 365116 (U2AF1), Accession No. Hs 368084 (LRPPRC), Accession No. Hs 368149 (CCT7), Accession No. Hs 368157 (PYGB), Accession No. Hs 368240 (DYRK1A), Accession No. Hs 68264 (PPP2R5C), Accession No. Hs 368376 (SRPR), Accession No. Hs 368402 (LOC51337), Accession No. Hs 368404 (EXT2), Accession No. Hs 368525 (PDLIM1), Accession No. Hs 368598 (LEREPO4), Accession No. Hs 368934 (MGC40157), Accession No. Hs 368985 (TRIP12), Accession No. Hs 369017 (RAB2), Accession No. Hs 369052 (SELT), Accession No. Hs 369068 (DNCLI2), Accession No. Hs 369125 (PSMD14), Accession No. Hs 369285 (DKFZP434B168), Accession No. Hs 369356 (MLL5), Accession No. Hs 369606 (CPSF6), Accession No. Hs 369607 (GAK), Accession No. Hs 369614 (COPS2), Accession No. Hs 369615 (FLJ20551), Accession No. Hs 369761 (DAZAP2), Accession No. Hs 369785 (MGC2749), Accession No. Hs 369920 (RAP1B), Accession No. Hs 370024 (SEC31L1), Accession No. Hs 370247 (APLP2), Accession No. Hs 370292 (BCCIP), Accession No. Hs 370312 (FNTA), Accession No. Hs 370408 (COMT), Accession No. Hs 370581 (CAP1), Accession No. Hs 370770 (XPO1), Accession No. Hs 370771 (CDKN1A), Accession No. Hs 370895 (RPN2), Accession No. Hs 370927 (PRO1855), Accession No. Hs 370937 (TAPBP), Accession No. Hs 371001 (EIF3S9), Accession No. Hs 371416 (CARM1), Accession No. Hs 371563 (RAB14), Accession No. Hs 371788 (DKFZP547E1010), Accession No. Hs 371889 (ATP1A1), Accession No. Hs 372003 (C9orf10), Accession No. Hs 372050 (SMAP-5), Accession No. Hs 372286 (CUL3), Accession No. Hs 372331 (SPTAN1), Accession No. Hs 372541 (KBTBD2), Accession No. Hs 372616 (ARL1), Accession No. Hs 372914 (NDRG1), Accession No. Hs 373550 (TGIF), Accession No. Hs 373741 (HM13), Accession No. Hs 373763 (HNRPR), Accession No. Hs 373952 (CAMTA2), Accession No. Hs 373959 (VGLL4), Accession No. Hs 374043 (ASXL1), Accession No. Hs 374257 (SIAT4A), Accession No. Hs 374378 (CKS1B), Accession No. Hs 374477 (EWSR1), Accession No. Hs 374503 (MORF4L1), Accession No. Hs 374588 (RPL17), Accession No. Hs 374596 (TPT1), Accession No. Hs 374650 (IFITM3), Accession No. Hs 374973 (PRPF4), Accession No. Hs 375001 (TLN1), Accession No. Hs 375108 (CD24), Accession No. Hs 375217 (RNF31), Accession No. Hs 376046 (BTN3A2), Accession No. Hs 376933 (GUK1), Accession No. Hs 377155 (LYRIC), Accession No. Hs 378103 (RPS5), Accession No. Hs 378532 (HBS1L), Accession No. Hs 378808 (eIF2A), Accession No. Hs 380403 (PCGF4), Accession No. Hs 380774 (DDX3X), Accession No. Hs 380953 (RPL38), Accession No. Hs 380973 (SUMO2), Accession No. Hs 381008 (HLA-E), Accession No. Hs 381058 (KIAA0146), Accession No. Hs 381072 (PPIF), Accession No. Hs 381123 (RPL21), Accession No. Hs 381126 (RPS14), Accession No. Hs 381189 (CBX3), Accession No. Hs 381219, Accession No. Hs 381256 (GLTP), Accession No. Hs 382044 (MRPS2), Accession No. Hs 382168 (NCOA3), Accession No. Hs 385913 (ANP32E), Accession No. Hs 385986 (UBE2B), Accession No. Hs 386434 (ANXA7), Accession No. Hs 386465 (CHERP), Accession No. Hs 386939 (USP7), Accession No. Hs 387208 (FAU), Accession No. Hs 387804 (PABPC1), Accession No. Hs 388034 (RXRB), Accession No. Hs 388654 (ATP6V1G1), Accession No. Hs 388664 (RPL11), Accession No. Hs 388739 (XRCC5), Accession No. Hs 388927 (YY1), Accession No. Hs 388956 (C19orf22), Accession No. Hs 389037 (MCM3APAS), Accession No. Hs 389107 (ATP6V0C), Accession No. Hs 389171 (PINK1), Accession No. Hs 389649 (DDX48), Accession No. Hs 389734 (TCEAL8), Accession No. Hs 389996 (CHCHD2), Accession No. Hs 390667 (GSTK1), Accession No. Hs 393201 (ACTR2), Accession No. Hs 395482 (PTK2), Accession No. Hs 396644 (PAIP2), Accession No. Hs 396740 (NIP30), Accession No. Hs 396783 (SLC9A3R1), Accession No. Hs 397609 (RPS16), Accession No. Hs 399800 (AKAP8L), Accession No. Hs 400295 (RPL30), Accession No. Hs 401509 (RBM10), Accession No. Hs 401903 (COX5A), Accession No. Hs 401929 (RPL10), Accession No. Hs 403917 (STK24), Accession No. Hs 404056 (EIF3S1), Accession No. Hs 404321 (GARS), Accession No. Hs 405144 (SFRS3), Accession No. Hs 405410 (OGT), Accession No. Hs 405514 (LOC284058), Accession No. Hs 405590 (EIF3S6), Accession No. Hs 405880 (MRPS21), Accession No. Hs 405942 (LOC339229), Accession No. Hs 406062 (NDUFA11), Accession No. Hs 406068 (UBE2M), Accession No. Hs 406096 (ZA20D2), Accession No. Hs 406277 (SF3A1), Accession No. Hs 406300 (RPL23), Accession No. Hs 406423 (SF3B2), Accession No. Hs 406510 (ATP5B), Accession No. Hs 406520 (LOC389541), Accession No. Hs 406534 (HMG20B), Accession No. Hs 406590 (PGR1), Accession No. Hs 406620 (RPS10), Accession No. Hs 406683 (RPS15), Accession No. Hs 406799 (RAB18), Accession No. Hs 406840 (SLC35A4), Accession No. Hs 407368 (C19orf13), Accession No. Hs 407580 (PKP4), Accession No. Hs 407995 (MIF), Accession No. Hs 408018 (RPL36), Accession No. Hs 408073 (RPS6), Accession No. Hs 408236 (TXNL5), Accession No. Hs 408257 (NDUFS6), Accession No. Hs 408293 (KAB), Accession No. Hs 408324 (FLJ10769), Accession No. Hs 408428 (CHES1), Accession No. Hs 408581 (SVIL), Accession No. Hs 408909 (GOLPH3), Accession No. Hs 409140 (ATP5O), Accession No. Hs 409223 (SSR4), Accession No. Hs 409230 (AGPAT1), Accession No. Hs 409834 (PHPT1), Accession No. Hs 410197 (IDH3G), Accession No. Hs 410596 (HAN11), Accession No. Hs 410817 (RPL13), Accession No. Hs 411480 (AUP1), Accession No. Hs 411641 (EIF4EBP1), Accession No. Hs 411847 (MAPK6), Accession No. Hs 412103 (EFHA1), Accession No. Hs 412117 (ANXA6), Accession No. Hs 412196 (ESRRBL1), Accession No. Hs 412433 (AIP), Accession No. Hs 412468 (KLHDC3), Accession No. Hs 412842 (C10orf7), Accession No. Hs 413036 (WBSCR22), Accession No. Hs 413482 (C21orf33), Accession No. Hs 414579 (SCOTIN), Accession No. Hs 415342 (KIAA1049), Accession No. Hs 416049 (TNPO2), Accession No. Hs 416436 (TRIM50A), Accession No. Hs 417004 (S100A11), Accession No. Hs 417029 (DERP6), Accession No. Hs 418123 (CTSL), Accession No. Hs 418175 (VPS28), Accession No. Hs 418233 (MRPL24), Accession No. Hs 418450 (MRPL11), Accession No. Hs 418533 (BUB3), Accession No. Hs 418668 (ATP5D), Accession No. Hs 419640 (PARK7), Accession No. Hs 420269 (COL6A2), Accession No. Hs 420272 (H2AFY), Accession No. Hs 421257 (RPL7), Accession No. Hs 421509 (CCT4), Accession No. Hs 422113 (ZNF511), Accession No. Hs 423935 (RDBP), Accession No. Hs 423968 (TTC11), Accession No. Hs 424126 (SERF2), Accession No. Hs 424908 (LSM5), Accession No. Hs 425777 (UBE2L6), Accession No. Hs 426296 (C10orf104), Accession No. Hs 426359 (DKFZp564J157), Accession No. Hs 429052 (ITGB1), Accession No. Hs 429353 (SEPN1), Accession No. Hs 429581 (RTN4), Accession No. Hs 429819 (PITPNA), Accession No. Hs 429839 (MGC23908), Accession No. Hs 430425 (GNB1), Accession No. Hs 430551 (IQGAP1), Accession No. Hs 430606 (CS), Accession No. Hs 430657 (ARF5), Accession No. Hs 430733 (CLNS1A), Accession No. Hs 431101 (GNG12), Accession No. Hs 431367 (C6orf55), Accession No. Hs 431498 (FOXP1), Accession No. Hs 431550 (MAP4K4), Accession No. Hs 431668 (COX6B1), Accession No. Hs 431850 (MAPK1), Accession No. Hs 431861 (PPP5C), Accession No. Hs 431926 NFKB1), Accession No. Hs 432121 (PRDX2), Accession No. Hs 432438 (EML4), Accession No. Hs 432491 (ESD), Accession No. Hs 432690 (SLC39A9), Accession No. Hs 432760 (CAPZB), Accession No. Hs 432898 (RPL4), Accession No. Hs 432976 (NR1H2), Accession No. Hs 433154 (PLSCR3), Accession No. Hs 433201 (CDK2AP1), Accession No. Hs 433222 (NPC2), Accession No. Hs 433291 (ARD1), Accession No. Hs 433307 (BCKDHA), Accession No. Hs 433343 (SRRM2), Accession No. Hs 433345, Accession No. Hs 433419 (COX4I1), Accession No. Hs 433512 (ACTR3), Accession No. Hs 433529 (RPS11), Accession No. Hs 433540 (DNAJC8), Accession No. Hs 433573 (Bles03), Accession No. Hs 433615 (TUBB2), Accession No. Hs 433701 (RPL37A), Accession No. Hs 433722 (KIAA1967), Accession No. Hs 33732 (CLK1), Accession No. Hs 433750 (EIF4G1), Accession No. Hs 433759 (BANF1), Accession No. Hs 433795 (SHC1), Accession No. Hs 433863 (PBP), Accession No. Hs 433901 (COX8A), Accession No. Hs 433951 (GPX4), Accession No. Hs 434102 (HMGB1), Accession No. Hs 434207 (HARS2), Accession No. Hs 434219 (ANKHD1), Accession No. Hs 434401 (ZNF638), Accession No. Hs 434937 (PPIB), Accession No. Hs 434953 (HMGB2), Accession No. Hs 434980 (APP), Accession No. Hs 435044 (TBC1D22A), Accession No. Hs 435064 (KIAA1608), Accession No. Hs 435120 (KIF1C), Accession No. Hs 435136 (TXN), Accession No. Hs 435166 (LBR), Accession No. Hs 435231 (ZFR), Accession No. Hs 435255 (UBXD1), Accession No. Hs 435326 (ACTL6A), Accession No. Hs 435512 (PPP3CA), Accession No. Hs 435535 (ZNF395), Accession No. Hs 435610 (WAC), Accession No. Hs 435741 (GCSH), Accession No. Hs 435759 (THAP4), Accession No. Hs 435771 (API5), Accession No. Hs 435841 (TNRC15), Accession No. Hs 435850 (LYPLA1), Accession No. Hs 435933 (PHF10), Accession No. Hs 435948 (ATAD1), Accession No. Hs 435952 (CDK5RAP1), Accession No. Hs 435974 (MTHFD1), Accession No. Hs 436035 (TUBA6), Accession No. Hs 436093 (BAT2), Accession No. Hs 436204 (ZNF289), Accession No. Hs 436298 (EMP1), Accession No. Hs 436405 (IDH3B), Accession No. Hs 436437 (ALDH2), Accession No. Hs 436446 (ARMET), Accession No. Hs 436500 (DBNL), Accession No. Hs 436568 (CD74), Accession No. Hs 436578 (POLR2F), Accession No. Hs 436657 (CLU), Accession No. Hs 436687 (SET), Accession No. Hs 436803 (VBP1), Accession No. Hs 437056 (SUPT5H), Accession No. Hs 437060 (CYCS), Accession No. Hs 437110 (ANXA2), Accession No. Hs 437178 (ACADVL), Accession No. Hs 437256 (GRINL1A), Accession No. Hs 437277 (MGAT4B), Accession No. Hs 437367 (GBAS), Accession No. Hs 437388 (PIGT), Accession No. Hs 437403 (PP), Accession No. Hs 437594 (RPLP2), Accession No. Hs 437638 (XBP1), Accession No. Hs 437779 (C11orf10), Accession No. Hs 437831 (C14orf32), Accession No. Hs 438072 (UNC84A), Accession No. Hs 438219 (GPS2), Accession No. Hs 438429 (RPS19), Accession No. Hs 438678 (TALDO1), Accession No. Hs 438720 (MCM7), Accession No. Hs 438970 (TBL1XR1), Accession No. Hs 438974 (CUTL1), Accession No. Hs 439480 (RBM5), Accession No. Hs 439481 (SUPT4H1), Accession No. Hs 439548 (FLJ22875), Accession No. Hs 439552, Accession No. Hs 439815 (HBXIP), Accession No. Hs 440382 (RFP), Accession No. Hs 440544 (CLIC4), Accession No. Hs 440599 (DDX1), Accession No. Hs 440604 (PSMD7), Accession No. Hs 440899 (TTYH3), Accession No. Hs 440932 (SEPT9), Accession No. Hs 440960 (RAD23A), Accession No. Hs 440961 (CAST), Accession No. Hs 441072 (POLR2L), Accession No. Hs 441550 (C20orf22), Accession No. Hs 442344 (IRS2), Accession No. Hs 442798 (RNF10), Accession No. Hs 443134 (GBA2), Accession No. Hs 443379 (PSMD11), Accession No. Hs 443837 (NPEPPS), Accession No. Hs 443914 (SOD1), Accession No. Hs 444279 (DKFZp761C169), Accession No. Hs 444356 (GRB2), Accession No. Hs 444468 (CTDSP1), Accession No. Hs 444472 (SDHC), Accession No. Hs 444569 (VMP1), Accession No. Hs 444673 (CRR9), Accession No. Hs 444724 (AZI2), Accession No. Hs 444818 (CGGBP1), Accession No. Hs 444931 (CRSP6), Accession No. Hs 444969 (C2orf4), Accession No. Hs 444986 (METAP2), Accession No. Hs 445081 (NS5ATP13TP2), Accession No. Hs 445351 (LGALS1), Accession No. Hs 445394 (VPS29), Accession No. Hs 445498 (SKIIP), Accession No. Hs 445511 (RIOK3), Accession No. Hs 445570 (CD63), Accession No. Hs 445803 (DC2), Accession No. Hs 445893 (KHDRBS1), Accession No. Hs 445977 (GTF3A), Accession No. Hs 446017 (WSB1), Accession No. Hs 446091 (WTAP), Accession No. Hs 446123 (CAPZA2), Accession No. Hs 446149 (LDHB), Accession No. Hs 446260 (PSMA6), Accession No. Hs 446336 (PXN), Accession No. Hs 446345 (FTH1), Accession No. Hs 446414 (CD47), Accession No. Hs 446427 (OAZ1), Accession No. Hs 446445 (YIF1), Accession No. Hs 446450 (ITM2B), Accession No. Hs 446574 (TMSB10), Accession No. Hs 446588 (RPS13), Accession No. Hs 446623 (HNRPL), Accession No. Hs 446628 (RPS4X), Accession No. Hs 446641 (ARAF), Accession No. Hs 446852 (EIF3S6IP), Accession No. Hs 447477 (ST13), Accession No. Hs 447492 (PGAM1), Accession No. Hs 447547 (VPS35), Accession No. Hs 48226 (RPLP0), Accession No. Hs 448588 (NGFRAP1), Accession No. Hs 448646 (RPL27A), Accession No. Hs 448879, Accession No. Hs 449114 (HNRPC), Accession No. Hs 449171 (HNRPK), Accession No. Hs 454534 (USF2), Accession No. Hs 454699 (IL6ST), Accession No. Hs 456507 (PKD1-like), Accession No. Hs 456557 (FLJ10597), Accession No. Hs 458320 (DC12), Accession No. Hs 458358 (TSPYL1), Accession No. Hs 458414 (IFITM1), Accession No. Hs 458458 (C19orf27), Accession No. Hs 458747 (ANP32A), Accession No. Hs 459106 (OAZIN), Accession No. Hs 459149 (BTBD1), Accession No. Hs 459174 (FLJ23790), Accession No. Hs 459211 (AKAP13), Accession No. Hs 459596 (MPG), Accession No. Hs 459649 (CLCN7), Accession No. Hs 459927 (PTMA), Accession No. Hs 459940 (LITAF), Accession No. Hs 460238 (SH3GLB2), Accession No. Hs 460317 (ALS4), Accession No. Hs 460336 (GGA2), Accession No. Hs 460468 (XPO6), Accession No. Hs 460499 (ATXN2L), Accession No. Hs 460574 (LOC124446), Accession No. Hs 460923 (CNOT1), Accession No. Hs 460929 (GOT2), Accession No. Hs 460978 (APPBP1), Accession No. Hs 461047 (G6PD), Accession No. Hs 461131 (CYB5-M), Accession No. Hs 461361 (CFDP1), Accession No. Hs 461379 (GABARAPL2), Accession No. Hs 461722 (HSPC176), Accession No. Hs 461777 (PCOLN3), Accession No. Hs 461896 (CRK), Accession No. Hs 461925 (RPA1), Accession No. Hs 462035 (UBE2G1), Accession No. Hs 462086 (RIP), Accession No. Hs 462306 (UBE2S), Accession No. Hs 462316 (TTC19), Accession No. Hs 462492 (USP22), Accession No. Hs 462550 (PIGS), Accession No. Hs 462956 (PPARBP), Accession No. Hs 462998 (IGFBP4), Accession No. Hs 463010 (SMARCE1), Accession No. Hs 463035 (FKBP10), Accession No. Hs 463041 (RERE), Accession No. Hs 463059 (STAT3), Accession No. Hs 463295 (CDC27), Accession No. Hs 463506 (AKAP1), Accession No. Hs 463702 (BCAS3), Accession No. Hs 463797 (C1orf33), Accession No. Hs 464071 (PGD), Accession No. Hs 464137 (ACOX1), Accession No. Hs 464210 (SYNGR2), Accession No. Hs 464336 (P4HB), Accession No. Hs 464438 (AGTRAP), Accession No. Hs 464472 (MRLC2), Accession No. Hs 464595 (PPP4R1), Accession No. Hs 464652 (TNFSF5IP1), Accession No. Hs 464912 (P15RS), Accession No. Hs 465224 (NARS), Accession No. Hs 465374 (EFHD2), Accession No. Hs 465498 (TXNL4A), Accession No. Hs 465529 (MIDN), Accession No. Hs 465543 (BTBD2), Accession No. Hs 465627 (MAP2K2), Accession No. Hs 465645 (C19orf10), Accession No. Hs 465808 (HNRPM), Accession No. Hs 465849 (PIN1), Accession No. Hs 465924 (SDHB), Accession No. Hs 466044 (PKN1), Accession No. Hs 466088 (TPM4), Accession No. Hs 466148 (NR2F6), Accession No. Hs 466471 (GPI), Accession No. Hs 466693 (SIRT2), Accession No. Hs 466766 (LTBP4), Accession No. Hs 466775 (SNRPA), Accession No. Hs 467084 (EIF4G3), Accession No. Hs 467097 (SNRP70), Accession No. Hs 467192 (PPP2R1A), Accession No. Hs 467279 (LENG4), Accession No. Hs 467284 (RPS9), Accession No. Hs 467408 (TRIM28), Accession No. Hs 467637 (CDC42), Accession No. Hs 467696 (HPCAL1), Accession No. Hs 467701 (ODC1), Accession No. Hs 467807 (LAPTM4A), Accession No. Hs 467824 (PUM2), Accession No. Hs 467960 (RAB10), Accession No. Hs 468018 (PPP1CB), Accession No. Hs 468415 (PIGF), Accession No. Hs 468442 (CALM2), Accession No. Hs 468760 (AFTIPHILIN), Accession No. Hs 469022 (DGUOK), Accession No. Hs 469171 (DKFZP564D0478), Accession No. Hs 469331 (STARD7), Accession No. Hs 469820 (RALB), Accession No. Hs 469863 (YWHAZ), Accession No. Hs 469925 (FLJ14346), Accession No. Hs 469970 (SFRS4), Accession No. Hs 470091 (YWHAE), Accession No. Hs 470233 (ARL5), Accession No. Hs 470417, Accession No. Hs 470477 (PTP4A2), Accession No. Hs 470577 (EIF2S2), Accession No. Hs 470588 (KPNA6), Accession No. Hs 470943 (STAT1), Accession No. Hs 471011 (SF3B1), Accession No. Hs 471104 (NOP5 / NOP58), Accession No. Hs 471207 (NDUFS1), Accession No. s 471441 (PSMB2), Accession No. Hs 471461 (ACSL3), Accession No. Hs 471593 (CAB39), Accession No. Hs 471768 (MGC4796), Accession No. Hs 471818 (M11S1), Accession No. Hs 471851 (HDLBP), Accession No. Hs 471873 (DTYMK), Accession No. Hs 471933 (FKBP1A), Accession No. Hs 471975 (C20orf116), Accession No. Hs 472010 (PRNP), Accession No. Hs 472024 (C20orf30), Accession No. Hs 472031 (UBE2D3), Accession No. Hs 472038 (CGI-94), Accession No. Hs 472056 (SYNCRIP), Accession No. Hs 472119 (MKKS), Accession No. Hs 472185 (NDUFS5), Accession No. Hs 472213 (RRBP1), Accession No. Hs 472330 (C20orf3), Accession No. Hs 472475 (MACF1), Accession No. Hs 472535 (AKIP), Accession No. Hs 472558 (SDBCAG84), Accession No. Hs 472651 (BLCAP), Accession No. Hs 472737 (TOP1), Accession No. Hs 473296 (TPD52L2), Accession No. Hs 473583 (NSEP1), Accession No. Hs 473648 (GART), Accession No. Hs 473721 (SLC2A1), Accession No. Hs 473761 (RTN3), Accession No. Hs 473788 (OTUB1), Accession No. Hs 474005 (SUMO3), Accession No. Hs 474010 (PTTG1IP), Accession No. Hs 474053 (COL6A1), Accession No. Hs 474083 (B4GALT2), Accession No. Hs 474213 (UFD1L), Accession No. Hs 474584 (AKR1A1), Accession No. Hs 474643 (HSPC117), Accession No. Hs 474751 (MYH9), Accession No. Hs 474833 (CSNK1E), Accession No. Hs 474914 (RUTBC3), Accession No. Hs 474938 (SLC25A17), Accession No. Hs 474949 (RBX1), Accession No. Hs 474982 (ACO2), Accession No. Hs 475125 (ATXN10), Accession No. Hs 475319 (LRRFIP2), Accession No. Hs 475382 (FLJ22405), Accession No. Hs 475392 (LOC55831), Accession No. Hs 475663 (RAB5A), Accession No. Hs 475733 (TOP2B), Accession No. Hs 475812 (SIMP), Accession No. Hs 476018 (CTNNB1), Accession No. Hs 476033 (TLP19), Accession No. Hs 476179 (SMARCC1), Accession No. Hs 476221 (IHPK2), Accession No. Hs 76231 (IMPDH2), Accession No. Hs 476308 (ALAS1), Accession No. Hs 476365 (SCP2), Accession No. Hs 476448 (FLNB), Accession No. Hs 476706 (MRPL37), Accession No. Hs 476930 (DKFZP564O123), Accession No. Hs 477157 (DULLARD), Accession No. Hs 477789 (ATP1B3), Accession No. Hs 477892 (GYG), Accession No. Hs 478000 (MBNL1), Accession No. Hs 478044 (PA2G4), Accession No. Hs 478553 (EIF4A2), Accession No. Hs 479208 (FBXL5), Accession No. Hs 479264 (LAP3), Accession No. Hs 479634 (SLC30A9), Accession No. Hs 479693 (SFRS11), Accession No. Hs 479728 (GAPD), Accession No. Hs 479747 (BCAR1), Accession No. Hs 479814 (POLR2B), Accession No. Hs 480073 (HNRPD), Accession No. Hs 480311 (PDLIM5), Accession No. Hs 480465 (SCYE1), Accession No. Hs 80653 (ANXA5), Accession No. Hs 481571 (UQCRH), Accession No. Hs 481720 (MYO10), Accession No. Hs 481898 (KAT3), Accession No. Hs 482144 (RPL26), Accession No. Hs 482363 (SLC30A5), Accession No. Hs 482526 (TINP1), Accession No. Hs 482868 (KIAA0372), Accession No. Hs 483036 (PJA2), Accession No. Hs 483067 (C5orf13), Accession No. Hs 483305 (HINT1), Accession No. Hs 483408 (PPP2CA), Accession No. Hs 483454 (CNN3), Accession No. Hs 483486 (JMJD1B), Accession No. Hs 484138 (FBXW11), Accession No. Hs 484188 (ATP6V0E), Accession No. 484242 (ETEA), Accession No. Hs 484288 (DDX41), Accession No. Hs 484363 (RNF130), Accession No. Hs 484551 (CPM), Accession No. Hs 484813 (DEK), Accession No. Hs 485155 (RPL35), Accession No. Hs 485195 (SORT1), Accession No. Hs 485246 (PSMA5), Accession No. Hs 485262 (MTCH1), Accession No. Hs 485365 (AHCYL1), Accession No. Hs 485616 (DST), Accession No. Hs 486542 (BCLAF1), Accession No. Hs 487027 (VIL2), Accession No. Hs 487054 (TCP1), Accession No. Hs 487635 (BZW2), Accession No. Hs 487774 (HNRPA2B1), Accession No. Hs 488171 (KIAA1068), Accession No. Hs 488181 (OGDH), Accession No. Hs 488307 (DKFZP564K0822), Accession No. Hs 488478 (FLJ10099), Accession No. Hs 488671 (BAZ1B), Accession No. Hs 489207 (ASNS), Accession No. Hs 489284 (ARPC1B), Accession No. Hs 489287 (CPSF4), Accession No. Hs 489336 (SYAP1), Accession No. Hs 489615 (PBEF1), Accession No. Hs 490203 (CALD1), Accession No. Hs 490394 (SSBP1), Accession No. Hs 490415 (ZYX), Accession No. Hs 490745 (DNAJB6), Accession No. Hs 490795 (CHR2SYT), Accession No. Hs 490874 (MTX1), Accession No. Hs 491336 (ELP3), Accession No. Hs 491359 (LMNA), Accession No. Hs 491440 (PPP2CB), Accession No. Hs 491494 (CCT3), Accession No. Hs 491597 (VDAC3), Accession No. Hs 491695 (UBE2V2), Accession No. Hs 491745 (TCEA1), Accession No. Hs 491988 (TRAM1), Accession No. Hs 492236 (WDR42A), Accession No. Hs 492314 (LAPTM4B), Accession No. Hs 492445 (EDD), Accession No. Hs 492599 (EIF3S3), Accession No. Hs 492805 (CGI-07), Accession No. Hs 493362 (AK3L1), Accession No. Hs 493750 (WDR40A), Accession No. Hs 494173 (ANXA1), Accession No. Hs 494419 (LAMP1), Accession No. Hs 494457 (NINJ1), Accession No. Hs 494604 (ANP32B), Accession No. Hs 494614 (XTP2), Accession No. Hs 494691 (PFN1), Accession No. Hs 494700 (CDW92), Accession No. Hs 494985 (FBXW2), Accession No. Hs 495039 (NDUFA8), Accession No. Hs 495349 (KIAA0515), Accession No. Hs 495471 (PMPCA), Accession No. Hs 495605 (CD99), Accession No. Hs 495851 (MGC4825), Accession No. Hs 495960 (ATP6AP2), Accession No. Hs 496068 (PCTK1), Accession No. Hs 496098 (DKFZp761A052), Accession No. Hs 496271, Accession No. Hs 496487 (ATF4), Accession No. Hs 496646 (IL13RA1), Accession No. Hs 496684 (LAMP2), Accession No. Hs 497183 (IVNS1ABP), Accession No. Hs 497599 (WARS), Accession No. Hs 497692 (C1orf48), Accession No. Hs 497893 (ENAH), Accession No. Hs 498239 (FH), Accession No. Hs 498313 (ADSS), Accession No. Hs 498317 (PNAS-4), Accession No. Hs 498455 (KIAA0217), Accession No. Hs 498548 (RBM17), Accession No. Hs 498727 (DHCR24), Accession No. Hs 499145 (YME1L1), Accession No. Hs 499158 (GGA1), Accession No. Hs 499594 (TIMM23), Accession No. Hs 499833 (C10orf74), Accession No. Hs 499891 (HNRPH3), Accession No. Hs 499925 (VPS26), Accession No. Hs 499960 (SARA1), Accession No. Hs 500067 (PPP3CB), Accession No. Hs 500101 (VCL), Accession No. Hs 500375 (ENTPD6), Accession No. Hs 500409 (GLUD1), Accession No. Hs 500546 (IDE), Accession No. Hs 500674 (SMBP), Accession No. Hs 500775 (ZNF207), Accession No. Hs 500842 (MGEA5), Accession No. Hs 500874 (CUEDC2), Accession No. Hs 501012 (ADD3), Accession No. Hs 501023 (MXI1), Accession No. Hs 501203 (TIAL1), Accession No. Hs 501293 (BSG), Accession No. Hs 501309 (CIRBP), Accession No. Hs 501353 (PLEKHJ1), Accession No. Hs 501376 (UROS), Accession No. Hs 501420 (NCLN), Accession No. Hs 501629 (IER2), Accession No. Hs 501684 (NAP1L4), Accession No. Hs 501735 (STIM1), Accession No. Hs 501853 (C11orf15), Accession No. Hs 501924 (USP47), Accession No. Hs 501991 (MLSTD2), Accession No. Hs 502302 (CAT), Accession No. Hs 502328 (CD44), Accession No. Hs 502461 (DGKZ), Accession No. Hs 502528 (NDUFS3), Accession No. Hs 502630 (C11orf31), Accession No. Hs 502659 (RHOC), Accession No. Hs 502705 (PRP19), Accession No. Hs 502745 (FADS2), Accession No. Hs 502769 (SLC3A2), Accession No. Hs 502773 (MTCBP-1), Accession No. Hs 502823 (PRDX5), Accession No. Hs 502829 (SF1), Accession No. Hs 502836 (ARL2), Accession No. Hs 502842 (CAPN1), Accession No. Hs 502872 (MAP3K11), Accession No. Hs 502876 (RHOB), Accession No. Hs 503093 (ZFP36L2), Accession No. Hs 503222 (RAB6A), Accession No. Hs 503251 (PME-1), Accession No. Hs 503597 (HSPC148), Accession No. Hs 503709 (PORIMIN), Accession No. Hs 503716 (MGC2714), Accession No. Hs 503787 (DARS), Accession No. Hs 504237 (ITM1), Accession No. Hs 504517 (RPS27), Accession No. Hs 504613 (PTMS), Accession No. Hs 504620 (REA), Accession No. Hs 504687 (MYL9), Accession No. Hs 504828 (DDX47), Accession No. Hs 504895 (STRAP), Accession No. Hs 505033 (KRAS2), Accession No. Hs 505059 (PSMD4), Accession No. Hs 505625 (C12orf10), Accession No. Hs 505652 (COPZ1), Accession No. Hs 505676 (CIP29), Accession No. Hs 505705 (MYL6), Accession No. Hs 505806 (PBXIP1), Accession No. Hs 505824 (CGI-51), Accession No. Hs 506215 (RARS), Accession No. Hs 506325 (NUDT4), Accession No. Hs 06759 (ATP2A2), Accession No. Hs 506861 (DDX54), Accession No. Hs 507074 (KIAA0152), Accession No. Hs 507162 (FLJ12750), Accession No. Hs 507584 (MGC9850), Accession No. Hs 507680 (PFAAP5), Accession No. Hs 507910 (PGRMC2), Accession No. Hs 507916 (TGFB1I4), Accession No. Hs 508010 (FNDC3A), Accession No. Hs 508644 (FLJ10154), Accession No. Hs 509163 (KIAA1181), Accession No. Hs 509226 (FKBP3), Accession No. Hs 509264 (KLHDC2), Accession No. Hs 509414 (KTN1), Accession No. Hs 509622 (RGL2), Accession No. Hs 509736 (HSPCB), Accession No. Hs 509791 (ERH), Accession No. Hs 509909 (NUMB), Accession No. Hs 510087 (ENSA), Accession No. Hs 510328 (DDX24), Accession No. Hs 510402 (MCP), Accession No. Hs 511067 (FLJ10579), Accession No. Hs 511138 (DKFZP564G2022), Accession No. Hs 511149 (SNAP23), Accession No. Hs 511425 (SRP9), Accession No. Hs 511504 (TCF12), Accession No. Hs 511862, Accession No. Hs 511952 (CBX6), Accession No. Hs 512005 (ARPC3), Accession No. Hs 512465 (SURF4), Accession No. Hs 512525 (RPS17), Accession No. Hs 512607 (MIR16), Accession No. Hs 512640 (PRKCSH), Accession No. Hs 512661 (KIAA1160), Accession No. Hs 512676, Accession No. Hs 512693 (FLJ20859), Accession No. Hs 512756 (THAP7), Accession No. Hs 512815 (AP3D1), Accession No. Hs 512857 (CD151), Accession No. Hs 512867 (H63), Accession No. Hs 512908 (ARPP-19), Accession No. Hs 513043 (C15orf12), Accession No. Hs 513055 (REC14), Accession No. Hs 513057 (RANBP5), Accession No. Hs 513058 (TMED3), Accession No. Hs 513071 (MESDC1), Accession No. Hs 513083 (RPL9), Accession No. Hs 513141 (IDH2), Accession No. Hs 513145 (NEUGRIN), Accession No. Hs 513153 (FURIN), Accession No. Hs 513230 (MRPL28), Accession No. Hs 513242 (RHOT2), Accession No. Hs 513261 (C16orf34), Accession No. Hs 513266 (NDUFB10), Accession No. Hs 513470 (NFATC2IP), Accession No. Hs 513488 (MVP), Accession No. Hs 513490 (ALDOA), Accession No. Hs 513520 (BCKDK), Accession No. Hs 513522 (FUS), Accession No. Hs 513631 (ARL2BP), Accession No. Hs 513856 (DPH2L1), Accession No. Hs 513984 (FLII), Accession No. Hs 514012 (MAP2K3), Accession No. Hs 514036 (SDF2), Accession No. Hs 514038 (FLOT2), Accession No. Hs 514174 (JUP), Accession No. Hs 514196 (RPL27), Accession No. Hs 514211 (MGC4251), Accession No. Hs 514216 (CGI-69), Accession No. Hs 514220 (GRN), Accession No. Hs 514297 (FLJ13855), Accession No. Hs 514303 (PHB), Accession No. Hs 514435 (SF3B3), Accession No. Hs 514489 (WBP2), Accession No. Hs 514535 (LGALS3BP), Accession No. Hs 514581 (ACTG1), Accession No. Hs 514590 (HGS), Accession No. Hs 514819 (AP2B1), Accession No. Hs 514870 (ATP5F1), Accession No. Hs 514920 (NDP52), Accession No. Hs 514934 (CAPZA1), Accession No. Hs 515003 (C19orf6), Accession No. Hs 515005 (STK11), Accession No. Hs 515018 (GNA13), Accession No. Hs 515053 (AES), Accession No. Hs 515070 (EEF2), Accession No. Hs 515092 (CLPP), Accession No. Hs 515155 (MGC2803), Accession No. Hs 515162 (CALR), Accession No. Hs 515164 (GADD45GIP1), Accession No. Hs 515210 (DNAJB1), Accession No. Hs 515255 (LSM4), Accession No. Hs 515266 (RENT1), Accession No. Hs 515271 (SFRS14), Accession No. Hs 515329 (RPL22), Accession No. Hs 515371 (CAPNS1), Accession No. Hs 515406 (AKT2), Accession No. Hs 515417 (EGLN2), Accession No. Hs 515432 (DEDD2), Accession No. Hs 515472 (SNRPD2), Accession No. Hs 515475 (SYMPK), Accession No. Hs 515487 (CALM3), Accession No. Hs 515494 (SLC1A5), Accession No. Hs 515500 (SAE1), Accession No. Hs 515515 (KDELR1), Accession No. Hs 515517 (RPL18), Accession No. Hs 515524 (NUCB1), Accession No. Hs 515540 (PTOV1), Accession No. Hs 515550 (LOC284361), Accession No. Hs 515598 (PRPF31), Accession No. Hs 515607 (PPP1R12C), Accession No. Hs 515642 (GPSN2), Accession No. Hs 515785 (BLVRB), Accession No. Hs 515846 (RUVBL2), Accession No. Hs 515848 (HADHB), Accession No. Hs 515890 (YPEL5), Accession No. Hs 516075 (TIA1), Accession No. Hs 516077 (FLJ14668), Accession No. Hs 516087 (TEX261), Accession No. Hs 516111 (DCTN1), Accession No. Hs 516114 (WBP1), Accession No. Hs 516157 (MAT2A), Accession No. Hs 516450 (FLJ20297), Accession No. Hs 516522 (FLJ21919), Accession No. Hs 516539 (HNRPA3), Accession No. Hs 516587 (UBE2Q), Accession No. Hs 516633 (NCKAP1), Accession No. Hs 516711 (CHPF), Accession No. Hs 516790 (ARHGEF2), Accession No. Hs 516807 (STK25), Accession No. Hs 516826 (TRIB3), Accession No. Hs 516855 (CENPB), Accession No. Hs 517080 (SLC35C2), Accession No. Hs 517106 (CEBPB), Accession No. Hs 517134 (C20orf43), Accession No. Hs 517145 (ENO1), Accession No. Hs 517168 (TAGLN2), Accession No. Hs 517216 (PEA15), Accession No. Hs 517232 (PEX19), Accession No. Hs 517240 (IFNGR2), Accession No. Hs 517262 (SON), Accession No. Hs 517293 (F11R), Accession No. Hs 517338 (ATP6V1E1), Accession No. Hs 17342 (DEDD), Accession No. Hs 517356 (COL18A1), Accession No. Hs 517357 (DGCR2), Accession No. Hs 517421 (PCQAP), Accession No. Hs 517438 (ASCC2), Accession No. Hs 517517 (EP300), Accession No. Hs 517543 (PES1), Accession No. Hs 517582 (MCM5), Accession No. Hs 517622 (UNC84B), Accession No. Hs 517641 (L3MBTL2), Accession No. Hs 517666 (DIA1), Accession No. Hs 517731 (PP2447), Accession No. Hs 517768 (DKFZP564B167), Accession No. Hs 517792 (C3orf10), Accession No. Hs 517817 (MGC3222), Accession No. Hs 517821, Accession No. Hs 517888 (CRTAP), Accession No. Hs 517948 (DHX30), Accession No. Hs 517949 (MAP4), Accession No. Hs 517969 (APEH), Accession No. Hs 517981 (TUSC2), Accession No. Hs 518060 (ARL6IP5), Accession No. Hs 518123 (TFG), Accession No. Hs 518236 (SEC61A1), Accession No. Hs 518244 (RPN1), Accession No. Hs 518249 (ZNF9), Accession No. Hs 518250 (COPG), Accession No. Hs 518265 (H41), Accession No. Hs 518326 (SERP1), Accession No. Hs 518346 (SSR3), Accession No. Hs 518374 (QSCN6), Accession No. Hs 518424 (NDUFB5), Accession No. Hs 518460 (AP2M1), Accession No. Hs 518464 (PSMD2), Accession No. Hs 518525 (GLUL), Accession No. Hs 518551 (RPL31), Accession No. Hs 518608 (PP784), Accession No. Hs 518609 (ARPC5), Accession No. Hs 518750 (OCIAD1), Accession No. Hs 18805 (HMGA1), Accession No. Hs 518827 (CCNI), Accession No. Hs 519276 (MAPKAPK2), Accession No. Hs 519304 (PELO), Accession No. Hs 519346 (ERBB2IP), Accession No. Hs 519347 (SFRS12), Accession No. Hs 519520 (RPS25), Accession No. Hs 519523 (SERPINB6), Accession No. Hs 519557 (TMEM14C), Accession No. Hs 519718 (TTC1), Accession No. Hs 519756 (STK10), Accession No. Hs 519818 (MGAT1), Accession No. Hs 519909 (MARCKS), Accession No. Hs 519930 (C6orf62), Accession No. Hs 520026 (VARS2), Accession No. Hs 520028 (HSPA1A), Accession No. Hs 520037 (NEU1), Accession No. Hs 520070 (C6orf82), Accession No. Hs 520140 (SRF), Accession No. Hs 520189 (ELOVL5), Accession No. Hs 520205 (EIF2AK1), Accession No. Hs 520210 (KDELR2), Accession No. Hs 520287 (C6orf111), Accession No. Hs 520313 (CD164), Accession No. Hs 520383 (STX7), Accession No. Hs 520421 (PERP), Accession No. Hs 520459 (GTF2I), Accession No. Hs 520623 (C7orf27), Accession No. Hs 520640 (ACTB), Accession No. Hs 520740 (SCRN1), Accession No. Hs 520794 (YKT6), Accession No. Hs 520898 (CTSB), Accession No. Hs 520943 (WBSCR1), Accession No. Hs 520967 (MDH2), Accession No. Hs 520973 (HSPB1), Accession No. Hs 520974 (YWHAG), Accession No. Hs 521064 (ZNF655), Accession No. Hs 521151 (FLJ22301), Accession No. Hs 521289 (REPIN1), Accession No. Hs 521487 (MGC8721), Accession No. Hs 521640 (RAD23B), Accession No. Hs 521809 (COBRA1), Accession No. Hs 521903 (LY6E), Accession No. Hs 521924 (SIAHBP1), Accession No. Hs 521969 (NDUFB11), Accession No. Hs 521973 (WDR13), Accession No. Hs 522074 (DSIPI), Accession No. Hs 522110 (CREB3), Accession No. Hs 522114 (CLTA), Accession No. Hs 522310 (NANS), Accession No. Hs 522373 (GSN), Accession No. Hs 522394 (HSPA5), Accession No. Hs 522463 (EEF1A1), Accession No. Hs 522507 (FBXW5), Accession No. Hs 522584 (TMSB4X), Accession No. Hs 522590 (EIF1AX), Accession No. Hs 522632 (TIMP1), Accession No. Hs 522665 (MAGED2), Accession No. Hs 522675 (FLJ12525), Accession No. Hs 522752 (PSMD10), Accession No. Hs 522817 (BCAP31), Accession No. Hs 522819 (IRAK1), Accession No. Hs 522823 (EMD), Accession No. Hs 522932 (NCOA4), Accession No. Hs 522995 (EIF4EBP2), Accession No. Hs 523004 (PSAP), Accession No. Hs 523012 (DDIT4), Accession No. Hs 523054 (SMP1), Accession No. Hs 523131 (TRAPPC3), Accession No. Hs 523145 (DDOST), Accession No. Hs 523215 (NDUFB8), Accession No. Hs 523238 (NOLC1), Accession No. Hs 523262 (C1orf8), Accession No. Hs 523299 (EIF3S10), Accession No. Hs 523302 (PRDX3), Accession No. Hs 523560 (HSPCA), Accession No. Hs 523680 (SSRP1), Accession No. Hs 523789 (TncRNA), Accession No. Hs 523829 (POLD4), Accession No. Hs 523836 (GSTP1), Accession No. Hs 523852 (CCND1), Accession No. Hs 523875 (INPPL1), Accession No. Hs 524009 (AASDHPPT), Accession No. Hs 524081 (DPAGT1), Accession No. Hs 524084 (RNF26), Accession No. Hs 524161 (RSU1), Accession No. Hs 524171 (RAD52), Accession No. Hs 524183 (FKBP4), Accession No. Hs 524195 (ARHGAP21), Accession No. Hs 524214 (MLF2), Accession No. Hs 524219 (TPI1), Accession No. Hs 524271 (PHC2), Accession No. Hs 524367 (CBARA1), Accession No. Hs 524395 (TUBA3), Accession No. Hs 524464 (ATP5G2), Accession No. Hs 524502 (RNF41), Accession No. Hs 524530 (CTDSP2), Accession No. Hs 524590 (RAB21), Accession No. Hs 524599 (NAP1L1), Accession No. Hs 524690 (PPIE), Accession No. Hs 524788 (RAB35), Accession No. Hs 524809 (RSN), Accession No. Hs 524899 (SAP18), Accession No. Hs 524920 (ZFP91), Accession No. Hs 524969 (Ufm1), Accession No. Hs 525134 (FLJ20277), Accession No. Hs 525163 (ANKRD10), Accession No. Hs 525232 (LRP10), Accession No. Hs 525238 (C14orf119), Accession No. Hs 525330 (ARF6), Accession No. Hs 525391 (FLJ20580), Accession No. Hs 525527 (RER1), Accession No. Hs 525626 (PACS1L), Accession No. Hs 525899 (C6orf49), Accession No. Hs 526464 (PML), Accession No. Hs 526521 (MDH1), Accession No. Hs 527105 (HNRPDL), Accession No. Hs 527193 (RPS23), Accession No. Hs 527348 (AKAP9), Accession No. Hs 527412 (ASAH1), Accession No. Hs 527861 (OS-9), Accession No. Hs 527862 (PKD1), Accession No. Hs 527980 (DUT), Accession No. Hs 528050 (HARS), Accession No. Hs 528222 (NDUFS4), Accession No. Hs 528300 (PITRM1), Accession No. Hs 528305 (DDX17), Accession No. Hs 528572 (SCAM-1), Accession No. Hs 528668 (RPL6), Accession No. Hs 528780 (GSPT1), Accession No. Hs 528803 (UQCRC2), Accession No. Hs 529059 (EIF3S4), Accession No. Hs 529132 (SEPW1), Accession No. Hs 529244 (NCK2), Accession No. Hs 529280 (ANAPC7), Accession No. Hs 529303 (ARPC2), Accession No. Hs 529369 (AFAP), Accession No. Hs 529400 (IFNAR1), Accession No. Hs 529420 (UBE2G2), Accession No. Hs 529591 (TLOC1), Accession No. Hs 529618 (TFRC), Accession No. Hs 529631 (RPL35A), Accession No. Hs 529782 (VCP), Accession No. Hs 529798 (BTF3), Accession No. Hs 529862 (CSNK1A1), Accession No. Hs 529890 (CANX), Accession No. Hs 529892 (SQSTM1), Accession No. Hs 529957 (SEC63), Accession No. Hs 530096 (EIF3S2), Accession No. Hs 530118 (LUC7L2), Accession No. Hs 530291 (ANXA11), Accession No. Hs 530314 (SSNA1), Accession No. Hs 530331 (PDHA1), Accession No. Hs 530381 (PIM3), Accession No. Hs 530412 (PAI-RBP1), Accession No. Hs 530436 (STXBP3), Accession No. Hs 530479 (PMF1), Accession No. Hs 530687 (RNH), Accession No. Hs 530734 (MRPL16), Accession No. Hs 530753 (FLJ20625), Accession No. Hs 530823 (COPS7A), Accession No. Hs 530862 (PRKAG1), Accession No. Hs 531081 (LGALS3), Accession No. Hs 531089 (PSMA3), Accession No. Hs 531176 (SARS), Accession No. Hs 531330 (CBWD1), Accession No. Hs 531614 (BTBD14B), Accession No. Hs 531752 (RANBP3), Accession No. Hs 531856 (GAS5), Accession No. Hs 531876 (DNCL2A), Accession No. Hs 531879 (RAD1), Accession No. Hs 532359 (RPL5), Accession No. Hs 532399 (KIAA0663), Accession No. Hs 532755 (GTL3), Accession No. Hs 532790 (NMT1), Accession No. Hs 532793 (KPNB1), Accession No. Hs 532803 (HN1), Accession No. Hs 532826 (MCL1), Accession No. Hs 532853 (NDUFB7), Accession No. Hs 533030 (HRIHFB2122), Accession No. Hs 533059 (TUBB), Accession No. Hs 533122 (SFRS10), Accession No. Hs 533136 (LRPAP1), Accession No. Hs 533192 (TOMM20), Accession No. Hs 533222 (HSA9761), Accession No. Hs 533245 (DDX46), Accession No. Hs 533282 (NONO), Accession No. Hs 533308 (PPP2R5D), Accession No. Hs 533317 (VIM), Accession No. Hs 533437 (TCEB1), Accession No. Hs 533440 (WWP1), Accession No. Hs 533474 (PPP1R8), Accession No. Hs 533479 (LYPLA2), Accession No. Hs 533526 (ATRX), Accession No. Hs 533624 (H3F3A), Accession No. Hs 533712 (RBM4), Accession No. Hs 533732 (SRP14), Accession No. Hs 533771 (STUB1), Accession No. Hs 533782 (KRT8), Accession No. Hs 533977 (TXNIP), Accession No. Hs 533985 (EXOC7), Accession No. Hs 533986 (ZNF258), Accession No. Hs 534125 (HLA-C), Accession No. Hs 534168 (NDUFA1), Accession No. Hs 534212 (SEC22L1), Accession No. Hs 534255 (B2M), Accession No. Hs 534307 (CCND3), Accession No. Hs 534314 (EIF5A), Accession No. Hs 534326 (ITGB4BP), Accession No. Hs 534338 (PPP4C), Accession No. Hs 534346 (RPS7), Accession No. Hs 534350 (SMARCB1), Accession No. Hs 534453 (GRIM19), Accession No. Hs 534456 (ANAPC11), Accession No. Hs 534457 (C14orf166), Accession No. Hs 534473 (TOMM22), Accession No. Hs 534483 (MGC2941), Accession No. Hs 536275 (PACS1), Accession No. Hs 541269 (NDUFB9), Accession No. Hs 546248 (CTSD), Accession No. Hs 546250 (DNCI2), Accession No. Hs 546253 (FDFT1), Accession No. Hs 546261 (HNRPA1), Accession No. Hs 546269 (RPL10A), Accession No. Hs 546271 (PCBP2), Accession No. Hs 546286 (RPS3), Accession No. Hs 546289 (RPS12), Accession No. Hs 546290 (RPS18), Accession No. Hs 546291 (NET-5), Accession No. Hs 546339 (SMAP), Accession No. Hs 546356 (RPL13A), Accession No. Hs 546394 (HSPC016), Accession No. Hs 547759 (SSBP3), Accession No. Hs 549178 (C9orf86), Accession No. Hs 552590 (HTF9C), Accession No. Hs 553496 (PGM3), Accession No. Hs 553512 (C3F), Accession No. Hs 554767 (NUP88), Accession No. Hs 554776 (SREBF1), Accession No. Hs 554894 (FLJ12953), Accession No. Hs 554896 (MGC11257), Accession No. Hs 555194 (FAM36A), Accession No. Hs 555866 (C1QBP), Accession No. Hs 555873 (HNRPAB), Accession No. Hs 555875 (IDH3A), Accession No. Hs 555889 (PSMC2), Accession No. Hs 555890 (RBBP4), Accession No. Hs 555911 (RBM8A), Accession No. Hs 555969 (RIC8), Accession No. Hs 555971 (PP1201), Accession No. Hs 555973 (MRPS25), Accession No. Hs 555994 (LONP), Accession No. Hs 556267 (FBXL10), Accession No. Hs 556461 (NDUFV2), Accession No. Hs 556795 (PAICS), Accession No. Hs 557550 (NPM1), Accession No. Hs 558296 (ACP1), Accession No. Hs 558313 (COX6A1), Accession No. Hs 558322 (EEF1B2), Accession No. Hs 558325 (EIF5), Accession No. Hs 558328 (FKBP5), Accession No. Hs 558330 (FTL), Accession No. Hs 558338 (HSPE1), Accession No. Hs 558345 (IK), Accession No. Hs 558354 (LAMR1), Accession No. Hs 558360 (NDUFB4), Accession No. Hs 558361 (NME2), Accession No. Hs 558362 (NUMA1), Accession No. Hs 558376 (RAC1), Accession No. Hs 558381 (RNU65), Accession No. Hs 558382 (RPL15), Accession No. Hs 558383 (RPL18A), Accession No. Hs 558384 (RPL19), Accession No. Hs 558385 (RPL23A), Accession No. Hs 558386 (RPL34), Accession No. Hs 558388 (RPS3A), Accession No. Hs 558389 (RPS8), Accession No. Hs 558390 (RPS24), Accession No. Hs 558391 (RPS26), Accession No. Hs 558396 (SCD), Accession No. Hs 558424 (CSDA), Accession No. Hs 558426 (EIF3S5), Accession No. Hs 558429 (G10), Accession No. Hs 558431 (RPL14), Accession No. Hs 558442 (PDCD6IP), Accession No. Hs 558448 (TXNL2), Accession No. Hs 558453 (ATP5L), Accession No. Hs 558454 (NUDC), Accession No. Hs 558458 (COPS8), Accession No. Hs 558473 (C18orf10), Accession No. Hs 558499 (8D6A), Accession No. Hs 558511 (PSARL), Accession No. Hs 558521 (C2orf33), Accession No. Hs 558591 (ORMDL1), Accession No. Hs 558825 (PDE4DIP), Accession No. Hs 558995, Accession No. Hs 567260 (CD2BP2), Accession No. Hs 567263 (C1orf43), Accession No. Hs 567267 (ATP2C1) and Accession No. One or more genes selected from the group consisting of Hs 567279 (HCNGP). In addition, the detection reagent is characterized in that the primer pair and / or probe that can specifically amplify the candidate standard expression gene.

상기 프라이머쌍은 상기 유전자의 센스 서열에서 선택되는 10 ~ 50 bp 길이의 센스 프라이머 및 유전자의 안티센스 서열에서 선택되는 10 ~ 50 bp 길이의 안티센스 프라이머인 것이 바람직하다. 상기 센스 프라이머의 길이는 12 ~ 30 bp인 것이 바람직하며, 15 ~ 26 bp인 것이 더욱 바람직하며, 17 ~ 22 bp인 것이 가장 바람직하며 또한 상기 안티 센스 프라이머의 길이는 12 ~ 30 bp인 것이 바람직하며, 15 ~ 26 bp인 것이 더욱 바람직하며, 17 ~ 22 bp인 것이 가장 바람직하나, 상기 유전자를 증폭시킬 수 있는 프라이머라면 길이에 제한 받는 것은 아니다. 상기 프라이머쌍은 통상의 널리 알려진 프라이머 제작 프로그램을 이용하여 제작될 수 있으며, 상기 유전자를 증폭할 수만 있다면, 유전자상에서의 프라이머의 위치가 한정되는 것은 아니다.The primer pair is preferably a 10 to 50 bp long sense primer selected from the sense sequence of the gene and an antisense primer of 10 to 50 bp length selected from the antisense sequence of the gene. The length of the sense primer is preferably 12 to 30 bp, more preferably 15 to 26 bp, most preferably 17 to 22 bp and the length of the anti-sense primer is preferably 12 to 30 bp, , 15 to 26 bp is more preferable, and 17 to 22 bp is most preferred, but if the primer that can amplify the gene is not limited in length. The primer pair may be prepared using a conventional well-known primer manufacturing program, and as long as the gene can be amplified, the position of the primer on the gene is not limited.

상기의 방법으로 수득된 2,087개의 유전자를 FunCat(Functional Classification Catalogue, Version 2.0, Ruepp A et al., Nucleic Acids Res 32:5539-45, 2004)을 이용하여 분류한 결과, 이전 보고된 HKG(housekeeping gene)의 경우 대사 및 리보솜 단백질이 많이 포함된 것(Eisenberg E & Levanon EY, Trends Genet 19:362-5, 2003; Hsiao LL et al., Physiol Genomics 7:97-104, 2001)에 비해 단백질 운명(protein fate, 23%, 308/1318) 및 세포 운송(cellular transport, 21%, 273/1318)에 관여하는 단백질이 다수 포함되었다(도 2 참조). 또한, 상기 2,087개의 유전자에서 CpG 섬의 빈도를 분석한 결과, 이전 문헌 결과들(Larsen F et al., Genomics 13:1095-107, 1992; Ponger L et al., Genome Res 11:1854-60, 2001)과 유사하게 높은 빈도로 CpG 섬이 발견되었다(표 1 참조).2,087 genes obtained by the above method were classified using FunCat (Functional Classification Catalogue, Version 2.0, Ruepp A et al., Nucleic Acids Res 32: 5539-45, 2004), and previously reported HKG (housekeeping gene) ) Compared to protein-rich fates (Eisenberg E & Levanon EY, Trends Genet 19: 362-5, 2003; Hsiao LL et al., Physiol Genomics 7: 97-104, 2001). protein fate, 23%, 308/1318) and a large number of proteins involved in cellular transport (21%, 273/1318) were included (see Figure 2). In addition, the analysis of the frequency of CpG islands in the 2,087 genes showed that the previous literature results (Larsen F et al., Genomics 13: 1095-107, 1992; Ponger L et al., Genome Res 11: 1854-60, Similarly, CpG islands were found with high frequency (see Table 1).

또한, 상기 유전자 검출은 마이크로어레이, SAGE(Serial Analysis of Gene Expression) 또는 qRT-PCR(quantitative real time PCR) 방법으로 측정될 수 있다. 상기 유전자 중에서 EST 및 LongSAGE에서는 EEF1A1이 가장 발현량이 많은 전사체로 ShortSAGE에서는 DGK1, HG-U133 마이크로어레이(microarray)에서는 RPL10A가 각각 가장 많이 발현되는 유전자였다(표 2 참조).In addition, the gene detection may be measured by microarray, SAGE (Serial Analysis of Gene Expression) or qRT-PCR (quantitative real time PCR) method. Among the genes, EEF1A1 was the most expressed in EST and LongSAGE, and DGK1 was expressed in ShortSAGE and RPL10A was expressed in HG-U133 microarray (see Table 2).

아울러, 상기와 같이 본 발명에 의해 선별되고 확인된 표준 발현 유전자들은 후보 표준 발현 유전자로서 발현량 안정성 검증을 통해 정량 대상 유전자의 발현량 분석 시 참고로 사용되는 표준발현 유전자들로 매우 유용하게 이용될 수 있다.In addition, the standard expression genes selected and identified by the present invention as described above are very useful as the standard expression genes used as a reference when analyzing the expression level of the gene to be quantitated by verifying expression stability as candidate standard expression genes. Can be.

또한, 본 발명은 1) 정량 대상 유전자를 증폭할 수 있는 프라이머쌍 및/또는 프로브를 이용하여 상기 정량 대상 유전자를 실시간 PCR로 증폭하고, 상기 조성물을 이용하여 시료에 포함되어 있는 후보 표준 발현 유전자를 실시간 PCR로 증폭하는 단계; 및In addition, the present invention 1) amplify the quantitative gene by real-time PCR using a primer pair and / or probe that can amplify the quantitative gene by real-time PCR, and using the composition to identify the candidate standard expression gene contained in the sample Amplifying by real-time PCR; And

또한, 본 발명은 상기 방법으로 선별된 후보 표준 발현 유전자의 변동계수(coefficient of variation, CV)를 측정하여 변동계수가 낮은 순으로 표준 발현 유전자를 선별하는 단계를 포함하는 유전자 발현량 측정 시 참고용 유전자의 선별 방법을 제공한다.In addition, the present invention is for reference when measuring the gene expression amount comprising the step of selecting the standard expression gene in the order of low coefficient of variation by measuring the coefficient of variation (CV) of the candidate standard expression gene selected by the above method Provides a method for screening genes.

상기 참고용 유전자는 인체의 대부분 조직에서 발현하는 유전자인 것을 특징으로 한다. 상기 참고용 유전자는 유전자 발현량의 상대적 정량 분석 시, 시료 간 발현량의 표준화에 사용할 수 있다. 또한, 상기 변동계수는 유전자 발현량의 변화가 클수록 값이 커지는 것을 특징으로 한다.The reference gene is characterized in that the gene expressed in most tissues of the human body. The reference gene may be used for standardization of the expression level between samples when the relative quantitative analysis of the gene expression amount. In addition, the coefficient of variation is characterized in that the larger the change in the amount of gene expression value.

본 발명의 후보 표준 발현 유전자(이하, 후보 ERG)의 선별방법에 의해 선별된 유전자 들 중, EST, ShortSAGE, LongSAGE 및 마이크로어레이를 포함한 각 데이터세트에서 각 유니젠 클러스터에 대해 CV를 계산하고 CV가 낮은 순으로 유전자를 정렬한 후, 후보 ERG 중에서 여러 조직에서 비교적 발현량이 일정한 표준 발현 유전자를 선별하였다. 그 결과, 각 데이터세트에서 CV가 낮은 순으로 선택한 400개(후보 ERG의 약 20%에 해당) 유전자들 중에서 4 개 데이터세트에 공통적인 13개 표준 발현 유전자를 선별하였다(도 1 및 표 3 참조). 상기 유전자에는 Accession No. Hs 500775(ZNF207), Accession No. Hs 446427(OAZ1), Accession No. Hs 530118(LUC7L2), Accession No. Hs 208597(CTBP1), Accession No. Hs 440382(TRIM27), Accession No. Hs 444279(GPBP1), Accession No. Hs 250009(ARL8B), Accession No. Hs 9589(UBQLN1), Accession No. Hs 253726(PAPOLA), Accession No. Hs 146806(CUL1), Accession No. Hs 533222(DIMT1L), Accession No. Hs 494985(FBXW2) 또는 Accession No. Hs 242458(SPG21) 등의 표준 발현 유전자(Endogenous Reference Genes; ERG)가 포함된다.Among the genes selected by the selection method of candidate standard expression genes (hereinafter, candidate ERGs) of the present invention, CVs are calculated for each Unigen cluster in each dataset including EST, ShortSAGE, LongSAGE, and microarray. After sorting the genes in order, standard expression genes were selected from among the candidate ERGs with relatively constant expression levels in various tissues. As a result, 13 standard expression genes common to four datasets were selected from 400 genes (approximately 20% of candidate ERGs) selected in the lowest CV order in each dataset (see Figure 1 and Table 3). ). The gene includes Accession No. Hs 500775 (ZNF207), Accession No. Hs 446427 (OAZ1), Accession No. Hs 530118 (LUC7L2), Accession No. Hs 208597 (CTBP1), Accession No. Hs 440382 (TRIM27), Accession No. Hs 444279 (GPBP1), Accession No. Hs 250009 (ARL8B), Accession No. Hs 9589 (UBQLN1), Accession No. Hs 253726 (PAPOLA), Accession No. Hs 146806 (CUL1), Accession No. Hs 533222 (DIMT1L), Accession No. Hs 494985 (FBXW2) or Accession No. Endogenous Reference Genes (ERGs) such as Hs 242458 (SPG21).

즉, 상기 표준 발현 유전자는 대다수의 조직에서 발현되는 2087개 유전자들 중에서 낮은 발현량 변동을 나타내는 유전자를 의미한다. 또한 상기 표준 발현 유전자는 세포 내 존재하는 대부분의 전사체의 낮은 발현량과 비슷한 발현량을 나타내는 것을 특징으로 한다.That is, the standard expression gene refers to a gene showing a low expression variation among 2087 genes expressed in the majority of tissues. In addition, the standard expression gene is characterized in that the expression level similar to the low expression level of most transcripts present in the cell.

상기 방법에 의해 선별된 13개의 표준 발현 유전자와 전통적으로 사용되는 표준 발현 유전자 13개(표 4 참조)를 유전자 발현량 및 CV 측면에서 비교한 결과, 전통적 표준 발현 유전자 중 6개는 모든 데이터세트에서 표준 발현 유전자보다 높은 평균 발현량을 보였고, 나머지 유전자들은 표준 발현 유전자와 비슷하거나 낮은 발현량을 나타내었다. 발현량은 전통적 표준 발현 유전자가 표준 발현 유전자보다 대체적으로 크고(도 6 참조), 내인성 표준 발현 유전자는 전통적인 내인성 표준 발현 유전자보다 상대적으로 낮은 CV를 보였다(도 7 참조).A comparison of the 13 standard expression genes selected by the above method and 13 standard expression genes (see Table 4) used in the past, in terms of gene expression and CV, shows that 6 of the traditional standard expression genes were found in all datasets. The average expression level was higher than that of the standard expression gene, and the remaining genes showed similar or lower expression levels than the standard expression gene. The expression amount was generally larger than the conventional standard expression gene (see FIG. 6), and the endogenous standard expression gene showed a relatively lower CV than the traditional endogenous standard expression gene (see FIG. 7).

또한 새로 발굴된 13개 유전자들의 표준 발현 유전자로서의 적합성을 더 확인하기 위해 유전자 발현에 영향을 줄 수 있는 유전자 카피 수 변이(gene copy number variation) 를 Database of Genomic Variants(//projects.tcag.ca/variation/)를 이용해 검토하였다(표 5 참조). 그 결과, 새로 발굴된 13개 유전자 중에는 OAZ1과 DIMT1L만 카피수 변이가 발견된 염색체 부위에 위치한 반면 기존의 전통적인 표준 발현 유전자들의 경우 상당수 유전자들(ACTB, GAPDH, PGK1, B2M, TBP, TFRC, ALAS1)이 유전자 카피수 변이가 알려진 부위에 위치하는 것으로 나타났다.In addition, gene copy number variation, which may affect gene expression, was further identified by Database of Genomic Variants ( //projects.tcag.ca/ variation / ) was used (see Table 5). As a result, among the 13 newly discovered genes, only OAZ1 and DIMT1L were located in the chromosomal region where copy number variation was found, whereas many of the existing standard expression genes (ACTB, GAPDH, PGK1, B2M, TBP, TFRC, ALAS1) were found. ) Is located at the site where the gene copy number variation is known.

더 나아가, 인체 조직 시료들 및 암 세포주들을 입수하여(표 6 참조) ERG의 정량적 실시간 RT-PCR(qRT-PCR)을 수행(표 7 참조)한 결과, 13개의 새로운 ERG는 인체 동결조직 및 암 세포주를 포함하는 48개 시료 모두에서 발현되었다(도 8 참조). 새로운 13개 ERG의 Cp는(Crossing Point, qRT-PCR 시 형광 시그날이 배경 시그날을 넘는 시점 cycle수를 가리킴) 19 - 29의 범위의 값을 보였다(도 9 참조). 또한, geNorm v3.4 소프트웨어(Vandesompele J et al., Genome Biol 3:RESEARCH0034, 2002) 및 NormFinder 소프트웨어(Andersen CL et al., Cancer Res 64:5245-50, 2004)를 이용하여 상기에서 실시한 PCR 효율(표 8 참조)을 고려하고 수학식 4를 이용하여 ERG의 발현 안정성을 분석한 결과, geNorm 프로그램 및 NormFinder 프로그램으로 계산된 유전자 발현 안정성은 새로 발굴된 13개 ERG 대다수가 기존의 전통적인 ERG보다 모두 높았다(표 9 참조). 표 9에서 발현 안정성 값(expression stability value, M by geNorm, S by NormFinder)가 낮은 값일수록 높은 발현 안정성을 의미한다.Furthermore, human tissue samples and cancer cell lines were obtained (see Table 6) and subjected to quantitative real-time RT-PCR (qRT-PCR) of ERG (see Table 7), resulting in 13 new ERGs for human frozen tissue and cancer. It was expressed in all 48 samples including cell lines (see FIG. 8). The Cp of the new 13 ERGs (crossing point, qRT-PCR, indicates the number of time cycles over which the fluorescence signal crosses the background signal) has a value ranging from 19 to 29 (see FIG. 9). In addition, PCR efficiency as described above using geNorm v3.4 software (Vandesompele J et al., Genome Biol 3: RESEARCH0034, 2002) and NormFinder software (Andersen CL et al., Cancer Res 64: 5245-50, 2004) Taking into account (see Table 8) and analyzing the expression stability of ERG using Equation 4, the gene expression stability calculated by the geNorm program and NormFinder program showed that the majority of the 13 newly discovered ERGs were higher than the conventional ERGs. (See Table 9). In Table 9, a lower expression stability value (M by geNorm, S by NormFinder) means higher expression stability.

상대적 발현량 =(1+E)^△Cp Relative expression amount = (1 + E) ^ΔCp

△Cp = 평균 Cp - 시료 Cp; ΔCp = mean Cp—sample Cp;

평균 Cp = 가장 낮은 Cp 값; 및 Mean Cp = lowest Cp value; And

E = PCR 효율 E = PCR efficiency

geNorm에 의해 계산된 값(M)과 마이크로어레이, LongSAGE 에서의 CV 간 유의한 상관관계는 없는 반면, EST, ShortSAGE 에서의 각 CV와 M 값 사이에는 유의한 상관관계가 있었다. 마찬가지로, NormFinder에 의해 계산된 안정성 값(S)도 마이크로어레이를 제외한 EST, ShortSAGE 및 LongSAGE와 유의한 상관관계를 나타냈으며, M 및 S 값 모두 ShortSAGE의 CV와 가장 높은 일치도를 보였다(표 10 참조). There was no significant correlation between the values calculated by geNorm (M) and microarray and CV in LongSAGE, while there was a significant correlation between CV and M values in EST and ShortSAGE. Similarly, the stability values (S) calculated by NormFinder showed significant correlations with EST, ShortSAGE and LongSAGE, except for microarrays, and M and S values showed the highest agreement with CV of ShortSAGE (see Table 10). .

상기와 같은 결과에 의해, 본 발명에 의해 발굴된 표준 발현 유전자(ERG)의 발현이 전통적인 유전자들보다 더 안정적임을 확인하였다.As a result, it was confirmed that the expression of the standard expression gene (ERG) discovered by the present invention is more stable than the conventional genes.

생체 내에 존재하는 전사체의 대부분이 그 발현량이 적은 것을 감안하면(Warrington JA et al., Physiol Genomics 2(3):143-147, 2000), 발현량이 큰 내인성 표준 발현 유전자를 표준화에 사용하는 것은 적합하지 않을 수도 있다. 동일한 선형 배율에서 측정하기 위해서는 목적 유전자와 비슷한 발현을 보이는 내인성 표준 발현 유전자 사용이 권고 된다(Szabo A et al., Genome Biol 5(8):R59, 2004; RocheAppliedScience Technical Note No. LC 15/2005). 따라서 본 발명에 의해 선택되고 확인된 내인성 표준 발현 유전자는, 전통적 내인성 표준 발현 유전자보다 낮은 변화량과 더불어, 상대적으로 낮은 발현량을 나타내므로 상기 내인성 표준 발현 유전자를 증폭시키는 표준화에 더 적합하고 보편적으로 사용될 수 있을 것으로 생각된다(Czechowski T et al., Plant Physiol 139(1):5-17, 2005).Given that most of the transcripts present in vivo have low expression levels (Warrington JA et al., Physiol Genomics 2 (3): 143-147, 2000), the use of endogenous standard expression genes with high expression levels for standardization It may not be suitable. In order to measure at the same linear magnification, it is recommended to use endogenous standard expression genes showing similar expression as the target gene (Szabo A et al., Genome Biol 5 (8): R59, 2004; RocheApplied Science Technical Note No. LC 15/2005). . Therefore, the endogenous standard expression genes selected and identified by the present invention exhibit relatively low expression levels with a lower amount of change than the traditional endogenous standard expression genes, and thus are more suitable and commonly used for standardization of amplifying the endogenous standard expression genes. It is thought to be possible (Czechowski T et al., Plant Physiol 139 (1): 5-17, 2005).

상기 참고용 유전자는 세포 내 존재하는 대부분의 전사체의 낮은 발현량과 비슷한 낮은 발현량을 나타내는 것을 특징으로 한다. 또한, 상기 참고용 유전자는 Accession No. Hs 500775(ZNF207), Accession No. Hs 446427(OAZ1), Accession No. Hs 530118(LUC7L2), Accession No. Hs 208597(CTBP1), Accession No. Hs 440382(TRIM27), Accession No. Hs 444279(GPBP1), Accession No. Hs 250009(ARL8B), Accession No. Hs 9589(UBQLN1), Accession No. Hs 253726(PAPOLA), Accession No. Hs 146806(CUL1), Accession No. Hs 533222(DIMT1L), Accession No. Hs 494985(FBXW2) 및 Accession No. Hs 242458(SPG21)으로 이루어진 군으로부터 선택된 하나 또는 둘 이상의 유전자인 것을 특징으로 한다. 상기 검출 시약은 참고용 유전자를 특이적으로 증폭할 수 있는 프라이머쌍 및/또는 프로브인 것을 특징으로 한다.The reference gene is characterized by a low expression level similar to the low expression level of most transcripts present in the cell. In addition, the reference gene is Accession No. Hs 500775 (ZNF207), Accession No. Hs 446427 (OAZ1), Accession No. Hs 530118 (LUC7L2), Accession No. Hs 208597 (CTBP1), Accession No. Hs 440382 (TRIM27), Accession No. Hs 444279 (GPBP1), Accession No. Hs 250009 (ARL8B), Accession No. Hs 9589 (UBQLN1), Accession No. Hs 253726 (PAPOLA), Accession No. Hs 146806 (CUL1), Accession No. Hs 533222 (DIMT1L), Accession No. Hs 494985 (FBXW2) and Accession No. It is characterized in that one or more genes selected from the group consisting of Hs 242458 (SPG21). The detection reagent is characterized in that the primer pair and / or probe that can specifically amplify the reference gene.

아울러, 상기 유전자 검출은 마이크로어레이, SAGE(Serial Analysis of Gene Expression) 또는 qRT-PCR(quantitative real time PCR) 방법으로 측정되는 것을 특징으로 한다.In addition, the gene detection may be measured by microarray, SAGE (Serial Analysis of Gene Expression) or qRT-PCR (quantitative real time PCR) method.

아울러, 본 발명은 1) 피검체로부터 수득된 게놈 DNA를 주형 DNA로 하여 대상 유전자를 증폭할 수 있는 프라이머쌍 및/또는 프로브를 이용하여 상기 대상 유전자를 실시간 PCR로 증폭하고, 상기 조성물을 이용하여 시료에 포함되어 있는 후보 표준 발현 유전자를 실시간 PCR로 증폭하는 단계; 및,In addition, the present invention 1) using a primer pair and / or a probe that can amplify a target gene using genomic DNA obtained from the subject as a template DNA amplify the target gene by real-time PCR, using the composition Amplifying the candidate standard expression gene included in the sample by real-time PCR; And,

본 발명의 방법은 '제로비율' 및 CV의 개념을 도입함으로써 상이한 데이터 베이스의 자료를 통합적으로 분석하여 표준 발현 유전자를 발굴하려는 목적에 적합하게 사용될 수 있고, 이로부터 발굴한 2,087개의 유전자는 대부분의 조직에서 발현되는 유지 유전자로서 발현 안정성 검증을 통해 향후 상대적 유전자 발현 분석 시 참고용 표준 발현 유전자로서 유용하게 이용될 수 있으며, 상기 2,087개의 유전자 중에서 13개의 표준 발현 유전자는 GAPDH 및 ACTB 같은 기존의 표준 발현 유전자보다 더 안정적인 발현과 동시에 더 낮은 발현량을 보임으로써, 상대적으로 발현량이 적은 생체 내의 보편적인 유전자들의 표준화에 더 적합할 것이다.The method of the present invention can be suitably used for the purpose of integrating analysis of data from different databases to discover standard expression genes by introducing the concept of 'zero ratio' and CV, and the 2,087 genes extracted from the As a maintenance gene expressed in tissues, it can be usefully used as a reference expression gene for future relative gene expression analysis through verification of expression stability. Among the 2,087 genes, 13 standard expression genes express existing standard expressions such as GAPDH and ACTB. By showing a more stable expression and a lower expression at the same time than a gene, it will be more suitable for the standardization of universal genes in a relatively small expression in vivo.

이하, 본 발명을 실시예 및 실험예에 의해 상세히 설명하다. 단 하기 실시예 및 실험예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용을 한정하지는 않는다.Hereinafter, the present invention will be described in detail by Examples and Experimental Examples. However, the following Examples and Experimental Examples are merely illustrative of the present invention and do not limit the content of the present invention.

<실시예 1> 유전자 발현 데이터세트의 구축Example 1 Construction of Gene Expression Dataset

EST(expressed sequence tag) 및 SAGE(serial analysis of gene expression) 인간 유전자 발현데이터는 공개 CGAP site(The Cancer Genome Project, //cgap.nci.nih.gov/)로부터 수집하였다. 마이크로어레이 유전자발현 자료는 Affymetrix Human Genome U133 array set을 기반으로 제작된 Gene Logic 사의 GeneExpress Oncology Datasuite^TM으로부터 입수하였다.EST (expressed sequence tag) and SAGE (serial analysis of gene expression) human gene expression data were collected from public CGAP site (The Cancer Genome Project, //cgap.nci.nih.gov/). Microarray gene expression data were obtained from GeneExpress Oncology Datasuite ^™ from Gene Logic, based on the Affymetrix Human Genome U133 array set.

CGAP 사이트로부터 수집하여 완성된 EST의 데이터세트는, Hs_LibData.dat(31/Oct/05) 파일에 있는 총 8,633개의 유전자 라이브러리(library) 중 하기의 조건, 즉,The data set of the completed EST collected from the CGAP site has the following conditions, among the total 8,633 gene libraries in the Hs_LibData.dat (31 / Oct / 05) file,

1) 표준화 작업을 거치지 않음(non-normalized); 및,1) non-normalized; And,

2) 시퀀스의 핵산 숫자가 >10,000을 만족하는 77개의 선택된 라이브러리를 포함하였다. 각 라이브러리의 EST 발현빈도는 Hs_ExprData.dat(31/Oct/05) 파일에서 입수하였다. 77개의 라이브러리를 포함하는 EST 데이터세트는 정상 및 종양 샘플을 포함한 서로 다른 29개 조직 및 26,117개의 유니젠 클러스터로 구성되었다.2) 77 selected libraries in which the nucleic acid number of the sequence satisfies> 10,000. EST expression frequency of each library was obtained from the file Hs_ExprData.dat (31 / Oct / 05). The EST dataset, comprising 77 libraries, consisted of 29 different tissues, including normal and tumor samples, and 26,117 Unigen clusters.

또한, 짤막한 절편의 SAGE(이하; ShortSAGE) 및 긴 절편의 SAGE(이하; LongSAGE) 데이터세트는, 38,290 개의 유니젠 클러스터를 포함하는 280개의 라이브러리(발현량 표시 파일명: Hs_short.frequencies.gz, 05/Dec/06) 및 21,868개의 유니젠 클러스터를 포함하는 46개의 라이브러리(발현량 표시 파일명: Hs_long.frequencies.gz, 05/Dec/06)를 각각 사용하여 생성되었다. 또한, 파일명 Hs_short.best_gene.gz(05/Dec/06) 및 파일명 Hs_long.best_gene.gz(05/Dec/06)를 이용하여 각 유니젠 클러스터에 SAGE 태그(tags)를 대응시켰다. ShortSAGE 및 LongSAGE 데이터세트는, 정상 및 종양샘플을 포함한 각각 28개 및 9개의 서로 다른 조직들에서의 SAGE 발현량의 정보들로 구성되었다.In addition, the short section SAGE (shortSAGE) and long section SAGE (LongSAGE) datasets include 280 libraries containing 38,290 Unizen clusters (expression display file name: Hs_short.frequencies.gz, 05 / Dec). / 06) and 46 libraries containing 21,868 Unizen clusters (expression display file name: Hs_long.frequencies.gz, 05 / Dec / 06), respectively. In addition, SAGE tags were associated with each Unigen cluster using the file name Hs_short.best_gene.gz (05 / Dec / 06) and the file name Hs_long.best_gene.gz (05 / Dec / 06). The ShortSAGE and LongSAGE datasets consisted of information on SAGE expression levels in 28 and 9 different tissues, respectively, including normal and tumor samples.

아울러, 마이크로어레이 데이터세트는 뇌(brain), 유방(breast), 대 장(colon), 식도(esophagus), 신장(kidney), 간(liver), 폐(lung), 림프절(lymph node), 난소(ovary), 췌장(pancreas), 전립샘(prostate), 직장(rectum) 및 위(stomach)를 포함하는, 13개 조직의 567 샘플들에서 얻어진 Affymetrix 유전자 발현자료를 포함하였다(표 11). Affymetrix(CA)의 HG-U133과 44,760개의 서로 다른 프로브세트들[총 44,928 프로브세트(probe sets)]로 마이크로어레이 데이터세트를 구성하였다.In addition, microarray datasets include brain, breast, colon, esophagus, kidney, liver, lung, lymph node, and ovaries. Affymetrix gene expression data obtained from 567 samples of 13 tissues, including ovary, pancreas, prostate, rectum and stomach, were included (Table 11). Microarray datasets were constructed with Affymetrix (CA) HG-U133 and 44,760 different probe sets (44,928 probe sets total).

<실시예 2> 후보 ERG의 선별Example 2 Selection of Candidate ERGs

본 발명은 실시예 1의 데이터세트를 통합하여 데이터세트 내의 유니젠 클러스터에 대해 각각의 발현량을 분석하여 인체의 대다수의 조직에서 발현하는 유지 유전자(housekeeping gene; 이하, HKG)의 탐색에 이용하였다.The present invention was integrated with the dataset of Example 1 to analyze the respective expression levels for the Unigen cluster in the dataset was used to search for housekeeping genes (hereinafter referred to as HKG) expressed in the majority of tissues of the human body.

EST 데이터세트의 유전자 발현량은, 수학식 1에 따라 특정 라이브러리 내에서 해당 유전자의 EST수를, 주어진 상기 라이브러리 내의 전체 EST 수로 나누고, 이에 1,000,000을 곱해 계산하였다.The gene expression amount of the EST dataset was calculated by dividing the number of EST of the gene in a specific library by the total number of ESTs in the given library according to Equation 1, and multiplying it by 1,000,000.

SAGE 데이터세트의 유전자 발현량은 수학식 2에 따라 특정 라이브러리 내에서 해당 유전자의 태그의 수를, 전체 태그 수로 나눈 값에, 1,000,000을 곱해서 계산하였다.The gene expression level of the SAGE dataset was calculated by multiplying the number of tags of the gene in a specific library by the total number of tags and multiplying it by 1,000,000 according to equation (2).

<수학식 1><Equation 1>

<수학식 2><Equation 2>

아울러, 마이크로어레이 데이터세트의 발현량은 어피메트릭 마이크로어레이 수트 5.0(Affymetric Microarray Suite 5.0)의 분석 알고리즘을 이용하여, 각 전사체(transcript)별로 계산된 신호 강도(signal intensity)로써 나타냈다. 또한, 데이터세트 내의 모든 라이브러리에서 특정 유전자의 발현량의 평균값을 평균 유전자 발현량으로 정의하였다.In addition, the expression level of the microarray dataset was expressed as signal intensity calculated for each transcript using an analysis algorithm of Affymetric Microarray Suite 5.0. In addition, the average value of the expression level of a specific gene in all libraries in the dataset was defined as the average gene expression level.

내인성 표준 발현 유전자를 발굴하기 위해 먼저 EST 및 SAGE 데이터세트를 사용하여 대부분의 조직에서 발현되는 HKG을 찾았다. 특정 유전자가 여러 조직에 걸쳐 대부분의 상황에서 발현되는 HKG일 가능성의 측정을 위해, EST와 SAGE의 데이터세트에 포함되는 모든 조직 중에서 상기 유전자가 발현되지 않는 조직의 비율을 계산하였다. 하나의 조직에 해당하는 여러 라이브러리 중 하나의 라이브러리에서라도 상기 유전자의 발현이 탐지되면 그 유전자는 해당 조직에서 발현되는 것으로 간주하고, 비교 대상이 되는 조직의 총 개수와 상기 유전자가 발현하지 않은 조직의 수의 비율로 제로 비율을 정의하였다. 즉, 제로 비율의 '0' 값은 데이터세트에 포함되어있는 모든 조직에서 발현된다는 것을, 반대로 '1'은 상기의 어떠한 조직에서도 발현되지 않는다는 것을 나타내었다.To identify endogenous standard expression genes, we first used the EST and SAGE datasets to find HKG expressed in most tissues. To determine the likelihood that a particular gene is an HKG expressed in most situations across multiple tissues, the percentage of tissues that do not express the gene among all tissues included in the EST and SAGE datasets was calculated. When the expression of the gene is detected in one of several libraries corresponding to one tissue, the gene is considered to be expressed in the tissue, and the total number of tissues to be compared and the number of tissues in which the gene is not expressed The ratio of zero is defined as the ratio of. In other words, a zero ratio '0' value is expressed in all tissues included in the dataset, whereas '1' indicates no expression in any of the tissues.

<수학식 3><Equation 3>

EST와 SAGE 데이터세트의 제로 비율에 대한 컷오프 값은, 2,600 프로브을 포함하는 Affymetrix U95A 칩(CA)을 사용하여, 47개의 서로 다른 인체 조직 및 세포주로부터 얻은 101개 시료를 대상으로 했던, 이전의 마이크로어레이 데이터로부터 확인된 575개의 HKG 대다수가 포함되도록(Eisenberg E and Levanon EY, Trends Genet, 19(7), 362-365, 2003), 데이터세트에 대하여 개별적으로 정하였다. EST에서는 제로 비율이 0.4 미만(EST, 4,027 유니젠 클러스터), ShortSAGE에서는 0.1 미만(4,758 유니젠 클러스터) 그리고 LongSAGE에서는 0.3 미만(4,569 유니젠 클러스터)인 유전자들이 각각 선별되었다. 상기 3개의 데이터세트에서 공통적인 2,087개 유전자가 선택되었다.The cutoff values for the zero ratios of the EST and SAGE datasets were the previous microarrays, which were subjected to 101 samples from 47 different human tissues and cell lines, using an Affymetrix U95A chip (CA) containing 2,600 probes. Datasets were individually determined to include the majority of the 575 HKG identified from the data (Eisenberg E and Levanon EY, Trends Genet, 19 (7), 362-365, 2003). In EST, genes with a zero ratio of less than 0.4 (EST, 4,027 Unigen clusters), shortSAGE less than 0.1 (4,758 Unigen clusters) and LongSAGE less than 0.3 (4,569 Unigen clusters) were selected. A common 2,087 genes were selected from the three datasets.

U95A에 의한 결과의 575개의 유전자 중, EST에서는 451(78.43%)개, ShortSAGE에서는 446(77.57%)개 및 LongSAGE에서는 446(77.57%)개의 유전자들이 HKG에 각각 포함되었다. 또한, 상기의 575개의 HKG 중 비록 몇몇 유전자들의 제로 비율이 예상외로 0.5보다 큰 값을 보이기도 했지만, 575개의 HKG 대부분은 본 발명의 데이터세트, EST, ShortSAGE 및 LongSAGE에서 낮은 제로 비율을 보였다. 또한, RPLP0, ACTB, PPIA, GAPDH, PGK1, B2M 및 TFRC같은 전통적인 내인성 표준 발현 유전자가 2,087개의 HKG에 포함되었다.Of the 575 genes resulting from U95A, 451 (78.43%) in EST, 446 (77.57%) in ShortSAGE and 446 (77.57%) in LongSAGE were included in HKG, respectively. In addition, although the zero ratio of some genes among the 575 HKGs was unexpectedly greater than 0.5, most of the 575 HKGs showed low zero ratios in the dataset, EST, ShortSAGE and LongSAGE of the present invention. In addition, traditional endogenous standard expression genes such as RPLP0, ACTB, PPIA, GAPDH, PGK1, B2M and TFRC were included in 2,087 HKGs.

또한, 마이크로어레이 데이터세트(Affymetrix HG-U133, CA)에서 EST와 SAGE 데이터세트에서 선별된 HKG의 평균 유전자 발현량 및 CV(%)를 구하였다. HKG에 해당하는 5,238 서로 다른 프로브세트(5317 단편, 1,990 유니젠 클러스터에 대한 유전자 발현 데이터)의 마이크로어레이 발현데이터가 얻어졌다.In addition, the average gene expression and CV (%) of HKG selected from the EST and SAGE datasets were obtained from the microarray dataset (Affymetrix HG-U133, CA). Microarray expression data of 5,238 different probe sets (5317 fragments, gene expression data for 1,990 Unigen clusters) corresponding to HKG were obtained.

유니젠 클러스터를 사용하여 각각의 데이터세트를 대응시켰고, HG-U133 칩 상에는 하나의 유전자 또는 유니젠 클러스터에 대해 다양한 발현 강도를 보이는 여러 프로브 세트가 대응되는 경우들이 있다. 이 경우 여러 프로브 세트 중 가장 낮은 CV를 보이는 프로브 세트를 선택하였다.Unigen clusters were used to map each dataset, and on the HG-U133 chip, there were cases where multiple probe sets with different expression intensities for one gene or Unigen cluster were mapped. In this case, the probe set showing the lowest CV among several probe sets was selected.

상기에서 수득한 2,087개의 HKG을 하기에서는 "후보 ERG" 또는 "후보 표준 발현 유전자"라고 칭하였다.The 2,087 HKGs obtained above were called "candidate ERG" or "candidate standard expression gene" in the following.

<실험예 1> 후보ERG의 특성분석Experimental Example 1 Characterization of Candidate ERG

<1-1> 후보 ERG의 기능적 분류<1-1> Functional Classification of Candidate ERGs

상기에서 수득된 2,087개의 유전자를 FunCat(Functional Classification Catalogue, Version 2.0, Ruepp, A., et al. Nucleic Acids Res, 32, 5539-45, 2004)을 이용하여 분류하였다. The 2,087 genes obtained above were classified using FunCat (Functional Classification Catalogue, Version 2.0, Ruepp, A., et al. Nucleic Acids Res, 32, 5539-45, 2004).

그 결과, 2,087개의 후보 ERG 중 1689개의 유니진 클러스터(UniGene clusters)의 GO 기(terms)가 MIPS(Munich Information Center for Protein Sequences) FunCat(Functional Catalogue)에 할당되었다. 1689개의 유니진 클러스터 중 생물학적 과정(biological process)에 해당하는 GO 기(terms)에 할당된 1318개의 유니진 클러스터가 기능적으로 분류되었다. 1318개 유전자들은 다양한 기본적인 세포 기능에 관여됨을 보여줬는데 이전 보고된 HKG의 경우 대사 및 리보솜 단백질이 많이 포함된 것(Eisenberg, E. and Levanon, E.Y. Trends Genet, 19, 362-5, 2003; Hsiao, L.L., et al. Physiol Genomics, 7, 97-104, 2001)에 비해 단백질 운명(protein fate, 23%, 308/1318) 및 세포 운송(cellular transport, 21%, 273/1318)에 관여하는 단백질이 다수 포함되었다(도 2). As a result, GO terms of 1689 UniGene clusters of 2,087 candidate ERGs were assigned to the MIPS (Munich Information Center for Protein Sequences) FunCat (Functional Catalog). Of the 1689 unigene clusters, 1318 unigene clusters assigned to GO terms corresponding to biological processes were classified functionally. 1318 genes have been shown to be involved in a variety of basic cellular functions, including previously reported high levels of metabolic and ribosome proteins in HKG (Eisenberg, E. and Levanon, EY Trends Genet, 19, 362-5, 2003; Hsiao, Compared with LL, et al. Physiol Genomics, 7, 97-104, 2001), proteins involved in protein fate (23%, 308/1318) and cellular transport (21%, 273/1318) Many were included (FIG. 2).

<1-2> 후보ERG의 CpG 섬(CpG island) 분석 <1-2> CpG island analysis of candidate ERG

본 발명의 ERG의 주석달린 전사 시작점(annotated transcription start)의 상위 서열(upstream sequences)은 UCSC 사이트(//hgdownload.cse.ucsc.edu/goldenPath/hg18/bigZips/)로부터 입수하였다. 전사 시작 자리로부터 1000, 2000 및 5000 bp 앞부분(bases upstream)의 CpG 섬을 CpGIE 프로그램(Wang, Y. and Leung, F. C. Bioinformatics, 20, 1170-7, 2004)을 이용하여 DNA 서열은 500 bp 보다 길고, G+C를 55% 포함하며, CpG 관찰값/기대값(Observed/Expected)이 0.65(Takai, D. and Jones, P. A. Proc Natl Acad Sci U S A, 99, 3740-5, 2002)인 것을 기준으로 하여 찾았다. The upstream sequences of the annotated transcription start of the ERG of the present invention were obtained from the UCSC site ( //hgdownload.cse.ucsc.edu/goldenPath/hg18/bigZips/) . DNA sequences longer than 500 bp using CpGIE programs (Wang, Y. and Leung, FC Bioinformatics, 20, 1170-7, 2004) for CpG islands 1000, 2000 and 5000 bp upstream from the start of transcription. , 55% G + C, and CpG Observed / Expected is 0.65 (Takai, D. and Jones, PA Proc Natl Acad Sci USA, 99, 3740-5, 2002) I found it.

후보 ERG 및 비-후보 ERG 간 CpG 섬을 가진 유전자 빈도의 통계학적 유의성은 Z-테스트(Z-test, R program, //www.R-project.org)를 이용하여 분석하였고, p<0.05를 유의한 것으로 간주하였다.Statistical significance of gene frequencies with CpG islands between candidate and non-candidate ERGs was analyzed using a Z-test (Z-test, R program, //www.R-project.org) and p <0.05 Considered significant.

대부분의 후보 ERG는 이전 문헌 결과들(Larsen, F., et al., Genomics, 13, 1095-107. 1992; Ponger, L., et al., Genome Res, 11, 1854-60. 42, 2001)과 유사하게 앞부분(upstream) 서열에서 높은 빈도로(1000 bp; 70%, 2000 bp; 73%, 5000bp; 76%) CpG 섬을 가지는 것으로 나타난 반면, 비-후보 ERG의 앞부분(upstream)에서는 낮은 빈도로 발견되었다(p<0.001, 표 1).Most candidate ERGs have been described in previous literature (Larsen, F., et al., Genomics, 13, 1095-107. 1992; Ponger, L., et al., Genome Res, 11, 1854-60. 42, 2001 Similarly), it appears to have a high frequency (1000 bp; 70%, 2000 bp; 73%, 5000 bp; 76%) CpG islands in the upstream sequence, while low in the upstream of the non-candidate ERG. Frequency was found (p <0.001, Table 1).

후보 ERG 및 비-후보 ERG의 전사 시작 자리 앞부분 서열(upstream sequences)에서 CpG 섬을 가진 유전자들의 비율 비교Comparison of the ratio of genes with CpG islands in the upstream sequences of transcription start sites of candidate ERG and non-candidate ERG 　 후보 ERG(개수=2002)Candidate ERG (Number = 2002) 비-후보 ERG(개수=14848)Non-candidate ERG (number = 14848) 　 앞부분 bpFront part bp 유전자 수Gene count CpG 섬을 가진 유전자 비율Gene Ratio with CpG Islets 유전자 수Gene count CpG 섬을 가진 유전자 비율Gene Ratio with CpG Islets p-값 p -value 50005000 15161516 76%76% 80368036 54%54% <0.001<0.001 20002000 14561456 73%73% 74107410 50%50% <0.001<0.001 10001000 14101410 70%70% 70487048 47%47% <0.001<0.001

<실험예 2> 상관분석Experimental Example 2 Correlation Analysis

두 군간 선형관계 측정의 척도인 상관계수를 이용한 분석을 시행하였으며, p<0.05 이면 유의한 것으로 간주하였다. 모든 계산 및 산포도(scatter plots)의 작성은 통계 분석용 프로그램인 R-패키지(R-package; //www.r-project.org)를 사용하여 수행하였다.An analysis was performed using the correlation coefficient, a measure of linear relationship between the two groups, and p <0.05 was considered significant. All calculations and scatter plots were made using R-package (//www.r-project.org), a statistical analysis program.

<2-1> 데이터세트 간 후보 ERG 상관분석<2-1> Candidate ERG Correlation Between Datasets

우선, 4개의 데이터세트로부터 선별된 후보 ERG를, 유전자 발현량 및 CV 측면에서 비교하기 위해, 피어슨(Pearson) 및 스피어만 순위(Spearman's rank) 상관분석을 각각 수행하였다. 그 결과, 도 3은 후보 ERG의 4개의 데이터세트 간 유전자발현에 대한 산포도(scatter plot)로, 4개의 데이터세트 간 유전자 발현에서 유의한 상관관계가 관찰되었다. ShortSAGE과 LongSAGE의 스피어만 상관계수 값이 0.853(p<0.0001)으로 가장 높았고, EST와 마이크로어레이는 가장 낮은 0.339(p<0.0001)의 스피어만 상관계수 값을 보여주었다.First, Pearson and Spearman's rank correlations were performed to compare candidate ERGs selected from four datasets in terms of gene expression and CV, respectively. As a result, FIG. 3 is a scatter plot of gene expression between four datasets of candidate ERGs, and a significant correlation was observed in gene expression between the four datasets. The Spearman correlation coefficient of ShortSAGE and LongSAGE was the highest at 0.853 (p <0.0001), and EST and microarray showed the lowest Spearman correlation coefficient of 0.339 (p <0.0001).

또한, 각 데이터세트별로 여러 라이브러리에서의 후보 ERG의 발현량의 일관성을 조사하기 위해 CV(%)를 구하여, 상기와 마찬가지로 피어슨(Pearson) 및 스피어만 순위(Spearman's rank) 상관분석을 각각 수행하였다. CV의 경우 비록 유의한 상관관계(p≤0.001)를 보여주긴 했지만, 유전자 발현의 상관관계보다는 데이터세트 간 낮은 상관관계(스피어만 상관계수<0.5)를 보여주었다(도 4).In addition, CV (%) was obtained to investigate the consistency of the expression levels of candidate ERGs in various libraries for each data set, and Pearson and Spearman's rank correlation analysis was performed as above. Although CV showed a significant correlation (p ≦ 0.001), it showed a lower correlation (Spearman correlation coefficient <0.5) between the datasets than the correlation of gene expression (FIG. 4).

상기의 결과는, 분석되는 시료의 종류나 수에 따라 발현량의 차이가 있을 수 있기 때문에, 각 데이터세트에 포함된 조직의 종류 및 시료 수의 차이에 기인한 것일 수도 있다.The above results may be due to the difference in the number of samples and the type of tissue included in each dataset, since the expression amount may vary depending on the type and number of samples to be analyzed.

<실험예 3> 후보 ERG와 비-후보 ERG 간 비교<Experiment 3> Comparison between candidate ERG and non-candidate ERG

후보 ERG 및 비-후보 ERG 간 평균 유전자 발현이 유의하게 차이가 나는지 t-검정을 사용하여 평가하였다. 도 5는 각 데이터세트의 후보 ERG 및 비-후보 ERG의 유전자 발현 분포를 나타낸다. 예상한 대로 후보 ERG의 평균 유전자 발현량이 비-후보 ERG보다 4개의 데이터세트 모두에서 유의하게 높았다(p<0.0001). 상기의 결과는 이전의 보고들과 일치한다(Eisenberg E and Levanon EY, Trends Genet, 19(7), 362-365, 2003).Significant differences in mean gene expression between candidate ERG and non-candidate ERG were assessed using the t-test. 5 shows the gene expression distribution of candidate ERG and non-candidate ERG in each dataset. As expected, mean gene expression of candidate ERGs was significantly higher in all four datasets than non-candidate ERGs (p <0.0001). The results are in agreement with previous reports (Eisenberg E and Levanon EY, Trends Genet, 19 (7), 362-365, 2003).

EST에서는 73.83-9324.87, ShortSAGE에서는 28.58 -4086.25, LongSAGE에서는 17.47-4621.60, HG-U133 마이크로어레이(microarray)에서는 1.47-18598.26 범위의 발현을 각각 보였다. 상당수의 리보솜(ribosomal) 단백질들은 4개의 데이터세트에서 모두 가장 발현량이 높은 유전자에 속하였다. EST 및 LongSAGE에서는 EEF1A1이, ShortSAGE에서는 DGK1이, HG-U133 마이크로어레이에서는 RPL10A이 각각 가장 많이 발현되는 유전자였다(표 2).EST expressions ranged from 73.83-9324.87, 28.58-4086.25 for ShortSAGE, 17.47-4621.60 for LongSAGE, and 1.47-18598.26 for HG-U133 microarray. Many of the ribosomal proteins belonged to the highest expression genes in all four datasets. EEF1A1 in EST and LongSAGE, DGK1 in ShortSAGE and RPL10A in HG-U133 microarray were the most expressed genes (Table 2).

Mean: 평균유전자 발현량, CV: 변동계수, 0's P: 제로비율Mean: Mean gene expression, CV: Coefficient of variation, 0's P: Zero ratio

<실시예 3> 표준 발현 유전자(ERG)의 발굴Example 3 Discovery of Standard Expression Gene (ERG)

표준 발현 유전자는 하기의 과정에 의해 상기 후보 표준 발현 유전자 중에서 추가로 선택하였다.Standard expression genes were further selected from the candidate standard expression genes by the following procedure.

우선, EST, ShortSAGE, LongSAGE 및 마이크로어레이(Affymetrix HG-U133, CA)을 포함한 각 데이터세트에서, 각각의 유니젠 클러스터에 대해 CV를 계산하고 CV가 낮은 순으로 유전자를 정렬하였다. CV가 낮은 순으로 각각의 데이터세트에서 400개의 유전자를 선별한 후(후보 ERG의 약 20%에 해당), 4개의 데이터세트에서 공통으로 선별된 내인성 표준 발현 유전자 13개를 발굴하였다(표 3). 내인성 표준 발현 유전자에는 Accession No. Hs 500775(ZNF207), Accession No. Hs 446427(OAZ1), Accession No. Hs 530118(LUC7L2), Accession No. Hs 208597(CTBP1), Accession No. Hs 440382(TRIM27), Accession No. Hs 444279(GPBP1), Accession No. Hs 250009(ARL8B), Accession No. Hs 9589(UBQLN1), Accession No. Hs 253726(PAPOLA), Accession No. Hs 146806(CUL1), Accession No. Hs 533222(DIMT1L), Accession No. Hs 494985(FBXW2) 및 Accession No. Hs 242458(SPG21)가 포함된다.First, in each dataset including EST, ShortSAGE, LongSAGE and Microarray (Affymetrix HG-U133, CA), CV was calculated for each Unigen cluster and the genes were sorted in descending order of CV. After selecting 400 genes from each dataset in descending order of CV (approximately 20% of candidate ERGs), 13 common endogenous standard expression genes were selected from four datasets (Table 3). . Endogenous standard expression genes include Accession No. Hs 500775 (ZNF207), Accession No. Hs 446427 (OAZ1), Accession No. Hs 530118 (LUC7L2), Accession No. Hs 208597 (CTBP1), Accession No. Hs 440382 (TRIM27), Accession No. Hs 444279 (GPBP1), Accession No. Hs 250009 (ARL8B), Accession No. Hs 9589 (UBQLN1), Accession No. Hs 253726 (PAPOLA), Accession No. Hs 146806 (CUL1), Accession No. Hs 533222 (DIMT1L), Accession No. Hs 494985 (FBXW2) and Accession No. Hs 242458 (SPG21) is included.

Gene Oncology는 Gene Ontology site (//www.geneontology.org/)에서 검색되었다.Gene Oncology was retrieved from the Gene Ontology site ( //www.geneontology.org/ ).

발굴된 유전자의 대부분(ZNF207, OAZ1, CTBP1, PAPOLA 및 FBXW2)은, 기본 세포 생리학적 과정 중 특히, 세포 대사(cellular metabolism)와 관련한 것들이었고, TRIM27 및 CUL1은 세포 증식(proliferation)과 관련한 유전자들이었다. OAZ1은 ShortSAGE 및 LongSAGE에서 가장 낮은 CV를 나타내었고, EST에서는 CTBP1 및 마이크로어레이에서는 ZNF207가 각각 가장 안정적인 발현을 보이는 유전자였다. 폴리아민 생합성(Polyamine biosynthesis)에 관여하는 OAZ1은 4개의 데이터세트에 걸쳐 가장 높은 발현량을 보였고, 또한, EST 데이터세트를 제외하고 상대적으로 낮은 변이를 보였다.Most of the genes discovered (ZNF207, OAZ1, CTBP1, PAPOLA and FBXW2) were related to cellular metabolism, in particular during basic cell physiological processes, while TRIM27 and CUL1 were related to cell proliferation. It was. OAZ1 had the lowest CV in ShortSAGE and LongSAGE, and ZNF207 was the most stable gene in EST in CTBP1 and microarray, respectively. OAZ1 involved in polyamine biosynthesis had the highest expression across four datasets and also showed relatively low mutations with the exception of the EST dataset.

<실험예 4> 데이터세트 간 표준 발현 유전자 상관분석 Experimental Example 4 Correlation of Standard Expression Genes Between Datasets

본 발명에 의해 선별된 내인성 표준 발현 유전자(ERG)를, 4개의 데이터세트 간 유전자 발현량 및 CV에 관해 비교하기 위해, 실험예 2와 동일한 방법으로 피어슨(Pearson) 및 스피어만 순위(Spearman's rank) 상관분석을 각각 수행하였다.In order to compare the endogenous standard expression gene (ERG) selected by the present invention with respect to gene expression amount and CV between four datasets, Pearson and Spearman's rank in the same manner as in Example 2 Correlation analyzes were performed respectively.

그 결과, 유전자 발현량은 일부 데이터 세트 간 유의한 상관관계를 보였지만 CV는 어느 데이터 세트 간에서도 유의한 상관관계를 보이지 않았다.As a result, gene expression showed a significant correlation between some data sets, but CV did not show a significant correlation between any data sets.

13개의 ERG 발현은 비록 스피어만 상관관계 측면에서는 EST-마이크로어레이 간(0.374, P=0.206), ShortSAGE-마이크로어레이 간(0.511, P=0.076) 유의성이 관찰되지 않았지만 피어슨 상관계수 측면에서 유의한 상관관계를 보였다(p<0.001). 데이터세트간 CV는 유의한 상관관계를 보이지 않았다(P>0.05). SAGES hort 및 LongSAGE에서는 CDC42 및 MYL6가 각각 ERG 중 모든 조직에서 발현되면서 가장 안정적인 발현을 보이는 유전자들이었다. HBP1 및 PSMC1은 EST 및 마이크로어레이에서 각각 가장 낮은 CV를 보였다.Thirteen ERG expressions were significantly correlated in terms of Pearson's correlation coefficient, although no significant differences were observed between EST-microarray (0.374, P = 0.206) and ShortSAGE-microarray (0.511, P = 0.076) in terms of Spearman correlation. Relationship was shown (p <0.001). The CV between the datasets showed no significant correlation (P> 0.05). In SAGES hort and LongSAGE, CDC42 and MYL6 were the most stable genes, expressed in all tissues of ERG, respectively. HBP1 and PSMC1 showed the lowest CV in EST and microarray, respectively.

<실험예 5> 유전자 발현 데이터세트에서의 새로운 표준 발현 유전자와 전통적 표준 발현유전자간 비교 Experimental Example 5 Comparison of New Standard Expression Genes and Traditional Standard Expression Genes in Gene Expression Datasets

본 발명에 의해 선별된 13개의 내인성 표준 발현 유전자와 전통적으로 사용되는 내인성 표준 발현 유전자 13개(표 4)를 유전자 발현량 및 CV 측면에서 비교하였다. 13 endogenous standard expression genes selected by the present invention and 13 conventionally used endogenous standard expression genes (Table 4) were compared in terms of gene expression and CV.

그 결과 분석된 전통적인 내인성 표준 발현 유전자 중 RPLP0, ACTB, PPIA, GAPDH, PGK1 및 B2M은 상대적으로 높은 발현량을 보였지만, GUSB, HPRT1, TBP, TFRC, ALAS1, H6PD 및 HMBS는 낮은 발현량을 보였다(도 6). 상기의 결과들은 qRT-PCR을 사용하여 잠재성 내인성 표준 발현 유전자의 mRNA 발현량을 측정한 이전 연구 결과와 일치한다(Radonic A et al., Biochem Biophys Res Commun, 313(4), 856-862, 2004). 발굴된 내인성 표준 발현 유전자의 발현량은 전통적 내인성 표준 발현 유전자의 저발현 군의 발현량과 비슷하거나 조금 높은 편이었다.As a result, RPLP0, ACTB, PPIA, GAPDH, PGK1, and B2M showed relatively high expression levels, but GUSB, HPRT1, TBP, TFRC, ALAS1, H6PD, and HMBS showed low expression levels. 6). These results are consistent with previous studies that measured the mRNA expression level of latent endogenous standard expression genes using qRT-PCR (Radonic A et al., Biochem Biophys Res Commun, 313 (4), 856-862, 2004). The expression level of the endogenous standard expression genes discovered was similar to or slightly higher than that of the low expression group of the traditional endogenous standard expression genes.

또한, 새로 발굴된 내인성 표준 발현 유전자는 몇몇 유전자들을 제외하고 전통적인 내인성 표준 발현 유전자보다 대체적으로 상대적으로 낮은 CV를 보여줬다(도 7). 즉 상대적으로 낮은 발현량 변화를 보여줬다. 상기의 결과는 본 발명에 의해 발굴된 내인성 표준 발현 유전자의 발현이 전통적 내인성 표준 발현 유전자들보다 더 안정적인 것을 나타낸다.In addition, newly discovered endogenous standard expression genes showed a relatively lower CV than traditional endogenous standard expression genes with the exception of some genes (FIG. 7). In other words, it showed a relatively low expression change. The above results indicate that the expression of endogenous standard expression genes discovered by the present invention is more stable than traditional endogenous standard expression genes.

<실험 예 6> 표준 발현 유전자들의 카피 수 변이 Experimental Example 6 Copy Number Variation of Standard Expression Genes

새로 발굴한 13개 ERGs의 유전자 카피수 변이(gene copy number variations)를 Database of Genomic Variants(//projects.tcag.ca/variation/)를 이용해 조사하였다. 새로 발굴된 13개 표준 발현 유전자중에서는 OAZ1과 DIMT1L 두 유전자들만이 카피수 변이(copy number variations)가 발견된 염색체 부위(chromosome region)에 위치하는 것으로 나타났다(표 5). 반면 전통적인 표준 발현 유전자들 중 상당수(ACTB, GAPDH, PGK1, B2M, TBP, TFRC, ALAS1) 가 카피수 변이가 알려진 유전자 부위(genomic locus)에 위치하였다(표 5). 즉 위 결과에 따르면 새로 발굴된 13개 표준 발현 유전자 중 2개 유전자를 제외한 나머지 유전자들은 유전자 카피수에 변이가 없을 가능성이 높으므로 상대적 유전자 증폭(gene amplification) 측정 시 참고 유전자로 사용될 수 있음을 제시한다.Gene copy number variations of 13 newly discovered ERGs were investigated using the Database of Genomic Variants ( //projects.tcag.ca/variation/ ). Of the 13 newly discovered standard expression genes, only two genes, OAZ1 and DIMT1L, were found in the chromosome region where copy number variations were found (Table 5). On the other hand, many of the standard expression genes (ACTB, GAPDH, PGK1, B2M, TBP, TFRC, ALAS1) were located in the genomic locus where copy number variation was known (Table 5). In other words, the above results suggest that the remaining genes except two of the 13 newly discovered standard expression genes may be used as reference genes when measuring relative gene amplification because there is a high possibility that there is no variation in the number of gene copies. do.

<실험 예 7> 표준 발현 유전자(ERG)의 검증Experimental Example 7 Validation of Standard Expression Gene (ERG)

<7-1> 정량적 실시간 RT-PCR(qRT-PCR)을 이용한 ERG의 발현량 검증<7-1> Verification of ERG Expression Level Using Quantitative Real-Time RT-PCR (qRT-PCR)

데이터세트로부터 선택된 ERG의 발현 안정성을 검증하기 위해 26개 동결 인체 조직, 60개 formalin-fixed, paraffin embedded(FFPE) 인체 조직 및 22개 인체 암 세포주를 포함한 총 108개 인체 시료들이 이용되었다(표 6). 60개 FFPE 조직에는 유방암 10개,정상 위장 8개, 위암 9개, 정상 난소 10개, 난소샘종 4개, 난소경계종양 9개 및 난소암 10개 조직이 각각 포함되었다, 이 조직들 및 세포주로부터 총 RNA를 추출하고, 동결 인체 조직 및 인체 암 세포주 시료 같은 경우 A260/280≥ 1.80 및 rRNA(28S/18S)≥ 1.0을 만족하는 RNA 시료들이 qRT-PCR에 이용되었다. 표준 방법에 따라 RNA로부터 cDNA를 합성한 후에 정량적 실시간 RT-PCR에 이용하기 위하여 1:3(cDNA:DW)으로 희석하였다. 사용한 PCR 프라이머 및 Universal Probe Library(UPL) 번호는 하기 표 7에 나타내었다.A total of 108 human samples, including 26 frozen human tissues, 60 formalin-fixed, paraffin embedded (FFPE) human tissues and 22 human cancer cell lines, were used to validate the expression stability of ERG selected from the dataset (Table 6). ). The 60 FFPE tissues included 10 breast cancers, 8 normal stomachs, 9 gastric cancers, 10 normal ovaries, 4 ovarian adenomas, 9 ovarian border tumors and 10 ovarian cancers, respectively. Total RNA was extracted and RNA samples satisfying A260 / 280 ≧ 1.80 and rRNA (28S / 18S) ≧ 1.0 for frozen human tissue and human cancer cell line samples were used for qRT-PCR. CDNA was synthesized from RNA according to standard methods and then diluted 1: 3 (cDNA: DW) for use in quantitative real-time RT-PCR. PCR primers used and Universal Probe Library (UPL) numbers are shown in Table 7 below.

qRT-PCR에서 사용한 전통적인 ERG는 기존 문헌들과 상업용 키트들에서의 사용빈도 및 본 발명의 데이터베이스에서 예측된 CV를 근거로 선택되었다. 가장 흔히 사용되는 ERG인 8개의 유전자(B2M, ACTB, GAPDH, HMBS, PPIA, HPRT1, TBP 및 H6PD)들은 Taqman human endogenous control plate(Applied Biosystems) 및 HKG selection kit(Roche Applied Science, Ohl F et, al., J Mol Med 83:1014-24, 2005; RocheAppliedScience Technical Note No. LC 15/2005; Applied Biosystems, 2001)와 같은 상업용 키트에 포함되어 있다. 각 유전자는 FAM-conjugate UPL probe(Rhoche Applied Science) 또는 custom-made specific probe(TIB MOLBIOL GmbH, Germany)를 이용하여 530 ㎚에서 측정되었다. 모든 PCR 반응은 표준 방법에 따라 Lightcycler 2.0(Roche Applied Science, USA)에서 수행되었다.The traditional ERG used in qRT-PCR was chosen based on the frequency of use in existing literature and commercial kits and the CV predicted in the database of the invention. The eight most commonly used ERG genes (B2M, ACTB, GAPDH, HMBS, PPIA, HPRT1, TBP, and H6PD) are available from Taqman human endogenous control plates (Applied Biosystems) and HKG selection kit (Roche Applied Science, Ohl F et, al. , J Mol Med 83: 1014-24, 2005; Roche Applied Science Technical Note No. LC 15/2005; Applied Biosystems, 2001). Each gene was measured at 530 nm using a FAM-conjugate UPL probe (Rhoche Applied Science) or a custom-made specific probe (TIB MOLBIOL GmbH, Germany). All PCR reactions were performed in Lightcycler 2.0 (Roche Applied Science, USA) according to standard methods.

각각의 유전자들의 PCR 효율은 위암세포주인 MKN74 세포주의 cDNA의 연속적 희석(serial dilution) 방법(Pfaffl MW et al., Nucleic Acids Res 29: e45, 2001)으로 측정하고, Lightcycler software 4.0(Roche Applied Science, USA)을 이용하여 계산하였으며, 90 ~ 100%의 값을 보였다(표 8).PCR efficiency of each gene was measured by serial dilution method of cDNA of gastric cancer cell line MKN74 cell line (Pfaffl MW et al., Nucleic Acids Res 29: e45, 2001), and Lightcycler software 4.0 (Roche Applied Science, USA), and showed a value of 90 to 100% (Table 8).

또한 각각의 조직 시료에서 프로브 각각의 PCR 효율을 LinRegPCR 프로그램으로 추정하였다(Ramakers C et al., Neurosci Lett 339:62-6, 2003). 모든 측정은 세 번 측정된 것이며 평균 Cp가 계산되었다. 실험적 변이를 최소화하기 위해 다른 조직 시료에서의 동일한 유전자는 동일 PCR 조건에서 측정하였다. H6PD의 Cp값은 폐, 간, 유방 및 신장 정상조직을 포함해서 4개 샘플들에서 측정되지 않았기 때문에 추후 계산에서 제외되었다. Cp 값의 CV는 3번 반복실험에서 5% 미만이었다.In addition, PCR efficiency of each probe in each tissue sample was estimated by the LinRegPCR program (Ramakers C et al., Neurosci Lett 339: 62-6, 2003). All measurements were taken three times and the average Cp was calculated. To minimize experimental variation, the same genes in different tissue samples were measured under the same PCR conditions. Cp values of H6PD were excluded from further calculations because they were not measured in four samples, including lung, liver, breast and kidney normal tissues. The CV of the Cp value was less than 5% in three replicates.

인체 동결 조직 및 암 세포주를 포함한 48개 시료 각각에서의 H6PD를 제외한 20개 유전자들의 발현은 도 8에 나타내었다. 13개의 새로운 ERG는 모든 48개 시료들에서 발현되었다. 7개의 전통적인 ERG는 넓은 발현 범위(Cp 값: 13.52 ~ 29.39)를 보인 반면, H6PD는 몇몇 조직에서 발현되지 않았다. 13개 ERG의 Cp는 18.90 ~ 28.79의 범위의 값을 보였다(도 9). 전통적인 ERG는 높게 발현되는 유전자들(median < 20 cycles)과 낮게 발현되는 유전자들(median > 23 cycles)로 나누어질 수 있었다. 높게 발현되는 유전자들에는 B2M, PPIA, GAPDH 및 ACTB이 포함되었으며 HPRT1, TBP, 및 HMBS는 발현량이 낮은 유전자들에 속하였다. OAZ1을 제외한 12개 ERG는 전통적인 ERG의 고발현군과 저발현군 사이의 발현 수준을 나타냈다. ERGs 중 ZNF2007은 가장 발현이 높은 유전자였으며 UBQLN1, CUL1이 그 다음으로 높았다. OAZ1은 가장 발현량이 낮은 유전자였다.Expression of 20 genes except H6PD in each of 48 samples, including human frozen tissue and cancer cell lines, is shown in FIG. 8. Thirteen new ERGs were expressed in all 48 samples. Seven traditional ERGs showed a wide range of expression (Cp values: 13.52-29.39), while H6PD was not expressed in some tissues. Cp of the 13 ERGs ranged from 18.90 to 28.79 (FIG. 9). Traditional ERG could be divided into high expressed genes (median <20 cycles) and low expressed genes (median> 23 cycles). Highly expressed genes included B2M, PPIA, GAPDH and ACTB and HPRT1, TBP, and HMBS belonged to low expression genes. Twelve ERGs, except OAZ1, showed expression levels between the high and low expression groups of traditional ERG. Among the ERGs, ZNF2007 was the most expressed gene, followed by UBQLN1 and CUL1. OAZ1 was the lowest expression gene.

<7-2> qRT-PCR 자료 근거 유전자 발현 안정성 분석을 이용한 ERG의 검증<7-2> Validation of ERG using qRT-PCR data-based gene expression stability analysis

유전자 발현 안정성 분석을 위해 geNorm v3.4 소프트웨어(Vandesompele J et al., Genome Biol 3, RESEARCH0034, 2002) 및 NormFinder 소프트웨어(Andersen CL et al., Cancer Res 64:5245-50, 2004)를 각각 사용하였다. geNorm 및 Normfinder 분석을 위해 표 8에 제시된 유전자들의 PCR 효율을 고려하여 Cp 값들을 상대적 발현량으로 변환하였다(GeNorm software manual, update on September 6, 2004. //medgen.ugnet.be/~jvdesomp/genorm/). 상대적 발현량은 하기 수학식 4와 같이 계산하였다.GeNorm v3.4 software (Vandesompele J et al., Genome Biol 3, RESEARCH0034, 2002) and NormFinder software (Andersen CL et al., Cancer Res 64: 5245-50, 2004) were used for gene expression stability analysis, respectively. . For geNorm and Normfinder analysis, the Cp values were converted to relative expression in consideration of the PCR efficiencies of the genes shown in Table 8 (GeNorm software manual, update on September 6, 2004. //medgen.ugnet.be/~jvdesomp/genorm / ). Relative expression was calculated as in Equation 4.

<수학식 4><Equation 4>

상대적 발현량 =(1+E)^△Cp Relative expression amount = (1 + E) ^ΔCp

△Cp = 평균 Cp - 시료 Cp; ΔCp = mean Cp—sample Cp;

평균 Cp = 가장 낮은 Cp 값; 및 Mean Cp = lowest Cp value; And

E = PCR 효율 E = PCR efficiency

geNorm 또는 NormFinder로 계산된 유전자 발현 안정성(하기에 geNorm으로 계산된 안정성은 M으로, NormFinder로 계산된 안정성은 S로 표기함)과 각각의 데이터세트에서 계산된 CV간 상관관계는 R 분석 소프트웨어(//www.R-project.org)를 이용하여 분석하였다.The correlation between the gene expression stability calculated with geNorm or NormFinder (hereafter, the stability calculated with geNorm is denoted by M and the stability calculated by NormFinder is denoted by S) and the CV calculated for each dataset are R analysis software (/ /www.R-project.org) .

모든 유전자들은 geNorm 프로그램으로 분석할 경우, geNorm 프로그램의 디폴트 리미트(default limit) 1.5 아래의 낮은 M 값(<0.9)을 보였으며 높은 발현 안정성을 나타내었다(표 9). 평균 발현 안정성 M의 값이 낮을수록 더 안정적인 발현을 보이는 것을 의미한다. GPBP1와 CUL1이 가장 안정적인 발현을 보이는 유전자들인 것으로 나타났다.분석된 유전자 중 B2M은 가장 높은 M 값(0.888)을 보이는 가장 발현량이 안정하지 않은 유전자였으며, 그 다음으로는 ACTB(0.843), HMBS(0.815), GAPDH(0.793) 순으로 불안정한 발현량을 보이는 유전자들인 것으로 나타났다.All genes showed low M values (<0.9) below the default limit 1.5 of the geNorm program and high expression stability when analyzed by the geNorm program (Table 9). Lower mean expression stability M means more stable expression. GPBP1 and CUL1 were the most stable genes, and B2M was the most unstable gene with the highest M value (0.888), followed by ACTB (0.843) and HMBS (0.815). ), GAPDH (0.793) in order to show the unstable expression of the genes.

다른 프로그램인 NormFinder를 이용했을 경우에는 TBP및 PAPOLA가 가장 상위 2개 순위에 해당하는 유전자들이었다(표 9). geNorm 결과와 유사하게 B2M, ACTB, GAPDH, HMBS 및 HPRT1은 새로운 ERG보다 덜 안정적인 것으로 나타났다. 두 프로그램 결과 모두 새로 발굴된 ERG 대부분이 기존의 전통적인 ERG보다 상대적으로 더 높은 발현 안정성을 보인다는 것을 증명하였다. 게다가 PCR 효율에 의해 계산된 상대적 발현량으로 유전자 발현 안정성을 LinRegPCR 프로그램을 이용해서 분석했을 경우에도 대부분의 ERG는 고전적인 ERG보다 더 안정적인 발현을 보였다.Using another program, NormFinder, TBP and PAPOLA were the two highest ranked genes (Table 9). Similar to the geNorm results, B2M, ACTB, GAPDH, HMBS and HPRT1 were less stable than the new ERG. Both program results demonstrated that most of the newly discovered ERGs showed relatively higher expression stability than conventional ERGs. Furthermore, even when the gene expression stability was analyzed using the LinRegPCR program with the relative expression amount calculated by PCR efficiency, most ERGs showed more stable expression than the classical ERGs.

geNorm에 의해 계산된 M 값과 마이크로어레이, LongSAGE 에서의 CV 간 유의한 상관관계는 없는 반면(p > 0.05) EST(피어슨 상관계수: 0.676, p=0.001), ShortSAGE(Pearson 상관계수: 0.659, p=0.002)에서의 각 CV와 M 값 사이에는 유의한 상관관계가 있었다. 마찬가지로, NormFinder에 의해 계산된 발현 안정성 값(S)도 마이크로어레이를 제외한 EST, ShortSAGE 및 LongSAGE과 유의한 상관관계를 나타냈다. M 및 S값 모두 Affymetrix 보다 EST, ShortSAGE의 CV와 더 높은 일치도를 보였다(표 10).There was no significant correlation between M value calculated by geNorm, microarray and CV in LongSAGE (p> 0.05), whereas EST (Pearson's correlation coefficient: 0.676, p = 0.001) and ShortSAGE (Pearson's correlation coefficient: 0.659, p = 0.002), there was a significant correlation between each CV and M value. Similarly, expression stability values (S) calculated by NormFinder also showed a significant correlation with EST, ShortSAGE and LongSAGE, except microarray. Both M and S values showed higher agreement with CV of EST and ShortSAGE than Affymetrix (Table 10).

더 나아가 13개 새로운 ERGs가 RNA의 분해(degradation)가 많은 조직에서도 사용될 수 있는지 시험해 보기 위해 60개 FFPE 시료들에서 qRT-PCR로 그 발현을 조사하였다. DIMT1L을 제외하고 모든 유전자들이 60개 모든 시료들에서 그 발현이 측정 가능하였고 고전적인 ERGs의 경우 18.85~ 33.02의 Cp 범위를 새로운 ERGs의 경우 23.33 내지 31.38의 Cp범위를 보였다(도 9). DIMT1L은 5개 시료들에서 증폭되지 않았기 때문에 이후 안정성 분석에서 제외되었다. 실험에 포함된 시료의 종류의 차이에도 불구하고 몇몇 유전자들을 제외하고 이전 48개 시료들에서 관찰된 것과 유사한 발현 양상이 관찰되었다. 상기 결과는 본 발명에서 새로 발굴된 ERG가 FFPE 시료에서의 유전자 발현 측정 시에도 사용될 수 있다는 것을 뒷받침한다. 48 개 시료에서와 마찬가지로 geNorm 및 NormFinder로 계산한 결과 대부분의 새로운 ERG가 기존 ERG 보다 낮은 안정성 값을 보이며 FFPE 시료에서도 안정성 측면에서 더 우월하다는 것이 증명되었다(표 9).Furthermore, the expression was examined by qRT-PCR in 60 FFPE samples to test whether 13 new ERGs could be used in many tissues for degradation of RNA. Except for DIMT1L, all genes were measurable in all 60 samples, with Cp ranging from 18.85 to 33.02 for classical ERGs and 23.33 to 31.38 for new ERGs (FIG. 9). DIMT1L was not amplified in five samples and was subsequently excluded from the stability analysis. Despite the differences in the types of samples included in the experiment, with the exception of some genes, expression patterns similar to those observed in the previous 48 samples were observed. The above results support that the newly discovered ERG in the present invention can also be used for measuring gene expression in FFPE samples. As with 48 samples, calculations with geNorm and NormFinder proved that most new ERGs had lower stability values than existing ERGs and were superior in stability to FFPE samples (Table 9).

*: 결장의 반지세포암중에서 정상부분; 및 *: Normal part of ring cell carcinoma of the colon; And

**: 융모양막염의 증거는 없지만 병소의 석회화가 있고, 정상의 두 배꼽동맥 및 하나의 정맥이 있는 미성숙 태반. **: Immature placenta with no evidence of chorioamnitis but with calcification of the lesion and two normal umbilical arteries and one vein.

실시간 PCR에 사용된 프라이머 및 Taqman 프로브Primers and Taqman Probes Used for Real-Time PCR 유전자 이름Gene name UPL 번호UPL number 프라이머primer 증폭산물 (bp)Amplification products (bp) 센스 프라이머 Sense primer 안티센스 프라이머Antisense primer GAPDH GAPDH 6060 서열번호 1: agccacatcgctcagaca SEQ ID NO: agccacatcgctcagaca 서열번호 2: gcccaatacgaccaaatcc SEQ ID NO: gcccaatacgaccaaatcc 6666 ACTBACTB 6464 서열번호 3: ccaaccgcgagaagatga SEQ ID NO: ccaaccgcgagaagatga 서열번호 4: ccagaggcgtacagggatag SEQ ID NO: ccagaggcgtacagggatag 9797 B2MB2M 4242 서열번호 5: ttctggcctggaggctatc SEQ ID NO: ttctggcctggaggctatc 서열번호 6: tcaggaaatttgactttccattcSEQ ID NO: 6 tcaggaaatttgactttccattc 8686 PPIAPPIA ## 서열번호 7: catctgcactgccaagactgag SEQ ID NO: 7: catctgcactgccaagactgag 서열번호 8: tgcaatccagctaggcatg SEQ ID NO: tgcaatccagctaggcatg 326326 HPRT1HPRT1 7373 서열번호 9: tgaccttgatttattttgcatacc SEQ ID NO: tgaccttgatttattttgcatacc 서열번호 10: cgagcaagacgttcagtcct SEQ ID NO: 10 cgagcaagacgttcagtcct 102102 HMBSHMBS 2626 서열번호 11: tgtggtgggaaccagctc SEQ ID NO: tgtggtgggaaccagctc 서열번호 12: tgttgaggtttccccgaatSEQ ID NO: 12: tgttgaggtttccccgaat 9292 TBPTBP 33 서열번호 13: gctggcccatagtgatcttt SEQ ID NO: 13: gctggcccatagtgatcttt 서열번호 14: cttcacacgccaagaaacagtSEQ ID NO: 14 cttcacacgccaagaaacagt 6060 H6PDH6PD 8989 서열번호 15: tggagatcatcatgaaagagaccSEQ ID NO: 15: tggagatcatcatgaaagagacc 서열번호 16: gcgaatgacaccgtactcctSEQ ID NO: 16: gcgaatgacaccgtactcct 7474 ZNF207ZNF207 2727 서열번호 17: ctgtttcctagcacagcacaaSEQ ID NO: 17 ctgtttcctagcacagcacaa 서열번호 18: ggtttgaaatctgtaccaacaggSEQ ID NO: 18 ggtttgaaatctgtaccaacagg 6565 OAZ1OAZ1 7474 서열번호 19: caccatgccgctcctaagSEQ ID NO: 19 caccatgccgctcctaag 서열번호 20: gagggagaccctggaactctSEQ ID NO: 20 gagggagaccctggaactct 6767 LUC7L2LUC7L2 8585 서열번호 21: cgatcacacagcaagaatcc SEQ ID NO: 21 cgatcacacagcaagaatcc 서열번호 22: agatcgatgtctgcgatgcSEQ ID NO: 22 agatcgatgtctgcgatgc 6060 CTBP1CTBP1 7777 서열번호 23: actgcgtgaccctgcact SEQ ID NO: 23 actgcgtgaccctgcact 서열번호 24: gccccttgtctcatctgcSEQ ID NO: 24 gccccttgtctcatctgc 8686 TRIM27TRIM27 77 서열번호 25: caggcacgagctgaactctSEQ ID NO: 25 caggcacgagctgaactct 서열번호 26: agctgctcaaactcccaaacSEQ ID NO: 26 agctgctcaaactcccaaac 7171 GPBP1GPBP1 44 서열번호 27: tcacttgaggcagaacacagaSEQ ID NO: 27 tcacttgaggcagaacacaga 서열번호 28: agcacatgtttcatcattttcac SEQ ID NO: 28 agcacatgtttcatcattttcac 7575 UBQLN1UBQLN1 7373 서열번호 29: gaatcctgaccttgctgcac SEQ ID NO: 29 gaatcctgaccttgctgcac 서열번호 30: ttgggagctgttgtctcattt SEQ ID NO: 30: ttgggagctgttgtctcattt 9292 ARL8BARL8B 8282 서열번호 31: aagcatgtgggagcggtat　 SEQ ID NO: 31 aagcatgtgggagcggtat 서열번호 32: cgatctgcagcatctatcatgt SEQ ID NO: 32 cgatctgcagcatctatcatgt 6666 PAPOLAPAPOLA 7878 서열번호 33: gctacgaagaccagtccattgSEQ ID NO: 33 gctacgaagaccagtccattg 서열번호 34: tgttggtcacagatgctgct SEQ ID NO: 34: tgttggtcacagatgctgct 9191 CUL1CUL1 6565 서열번호 35: gcgaggtcctcactcagcSEQ ID NO: 35 gcgaggtcctcactcagc 서열번호 36: ttctttctcaattagaatgtcaatgcSEQ ID NO: 36: ttctttctcaattagaatgtcaatgc 8686 DIMT1L DIMT1L 7777 서열번호 37: tccagtgttgtaaggatagaacctaag SEQ ID NO: 37 tccagtgttgtaaggatagaacctaag 서열번호 38: CcttactagaccatcccattcctSEQ ID NO: 38 Ccttactagaccatcccattcct 7575 FBXW2FBXW2 33 서열번호 39: cggctctgcagacttcact　 SEQ ID NO: 39 cggctctgcagacttcact 서열번호 40: ttgcacttctgcaaaactacct SEQ ID NO: 40 ttgcacttctgcaaaactacct 111111 SPG21SPG21 2121 서열번호 41: gatgtctttttccggcagatSEQ ID NO: 41 gatgtctttttccggcagat 서열번호 42: cgagatggtcccaataaactg SEQ ID NO: 42 cgagatggtcccaataaactg 8888

#: F-ttcttgctggtcttgccatTcctgga-p; T: TAMRA-표지; F: FAM-표지; P: 포스페이트.#: F-ttcttgctggtcttgccatTcctgga-p; T: TAMRA-labeled; F: FAM-label; P: phosphate.

각각 유전자들의 PCR 효율PCR efficiency of each gene 유전자명Gene name UPL 프로브 번호UPL probe number PCR 효율(희석)^* PCR efficiency (dilution) ^* PCR 효율(LinRegPCR)^** PCR efficiency (LinRegPCR) ^** GAPDH GAPDH 6060 1.8991.899 1.735±0.048(137)1.735 ± 0.048 (137) ACTBACTB 6464 2.0382.038 1.491±0.034(137)1.491 ± 0.034 (137) B2MB2M 4242 1.8681.868 1.717±0.068(140)1.717 ± 0.068 (140) PPIAPPIA ## 1.8771.877 1.773±0.058(142)1.773 ± 0.058 (142) HPRT1HPRT1 7373 1.8001.800 1.771±0.024(143)1.771 ± 0.024 (143) HMBSHMBS 2626 1.9541.954 1.431±0.031(143)1.431 ± 0.031 (143) TBPTBP 33 1.8261.826 1.447±0.038(142)1.447 ± 0.038 (142) H6PDH6PD 8989 1.8741.874 1.832±0.026(64)1.832 ± 0.026 (64) ZNF207ZNF207 2727 1.8691.869 1.648±0.018(142)1.648 ± 0.018 (142) OAZ1OAZ1 7474 2.0682.068 1.498±0.059(142)1.498 ± 0.059 (142) LUC7L2LUC7L2 8585 1.8291.829 1.709±0.047(143)1.709 ± 0.047 (143) CTBP1CTBP1 7777 2.0642.064 1.651±0.055(141)1.651 ± 0.055 (141) TRIM27TRIM27 77 1.9081.908 1.693±0.034(143)1.693 ± 0.034 (143) GPBP1GPBP1 44 1.8441.844 1.715±0.031(141)1.715 ± 0.031 (141) UBQLN1UBQLN1 7373 1.8641.864 1.723±0.027(143)1.723 ± 0.027 (143) ARL8BARL8B 8282 1.8381.838 1.499±0.074(139)1.499 ± 0.074 (139) PAPOLAPAPOLA 7878 1.8301.830 1.509±0.032(141)1.509 ± 0.032 (141) CUL1CUL1 6565 1.8101.810 1.695±0.027(139)1.695 ± 0.027 (139) DIMT1L DIMT1L 7777 1.9061.906 1.655±0.037(141)1.655 ± 0.037 (141) FBXW2FBXW2 33 1.8911.891 1.638±0.02(142)1.638 ± 0.02 (142) SPG21SPG21 2121 1.8261.826 1.636±0.021(142)1.636 ± 0.021 (142)

*: PCR 효율; MKN 74 위암세포의 cDNA로부터 연속 희석하고 Roche Lightcycler software 4.0으로 계산함;*: PCR efficiency; Serial dilution from cDNA of MKN 74 gastric cancer cells and calculated with Roche Lightcycler software 4.0;

**: PCR 효율; LinRegPCR(Ramakers et al., Neurosci Lett 339(1):62-66, 2003)을 이용하여 측정됨; 및,**: PCR efficiency; Measured using LinRegPCR (Ramakers et al., Neurosci Lett 339 (1): 62-66, 2003); And,

실시간 PCR 데이터에 근거해 geNorm 및 NormFinder에 의해 계산된 새로운 ERG 및 기존 ERG의 발현 안정성 Expression stability of new and existing ERGs calculated by geNorm and NormFinder based on real-time PCR data 48 시료48 samples 60 FFPE 시료* 60 FFPE Samples * GeNormGenorm 　　 NormFinderNormFinder 　 GeNormGenorm 　 NormFinderNormFinder 유전자gene 평균 발현 안정성MAverage expression stability M 　 유전자gene 안정성 수치SStability Figure S 유전자gene 평균 발현 안정성MAverage expression stability M 　 유전자gene 안정성 수치SStability Figure S GPBP1GPBP1 0.496 0.496 　 TBPTBP 0.2760.276 GPBP1 GPBP1 0.409 0.409 ARL8BARL8B 0.2330.233 CUL1CUL1 PAPOLAPAPOLA 0.2800.280 PAPOLAPAPOLA LUC7L2LUC7L2 0.2350.235 PAPOLAPAPOLA 0.536 0.536 CUL1CUL1 0.2870.287 ARL8BARL8B 0.437 0.437 OAZ1OAZ1 0.2470.247 TBPTBP 0.548 0.548 LUC7L2LUC7L2 0.2900.290 CTBP1 CTBP1 0.454 0.454 CTBP1CTBP1 0.2510.251 LUC7L2LUC7L2 0.565 0.565 CTBP1CTBP1 0.3120.312 LUC7L2 LUC7L2 0.483 0.483 UBQLN1UBQLN1 0.2730.273 TRIM27TRIM27 0.585 0.585 GPBP1GPBP1 0.3170.317 SPG21SPG21 0.509 0.509 SPG21SPG21 0.2800.280 FBXW2FBXW2 0.597 0.597 TRIM27TRIM27 0.3170.317 FBXW2FBXW2 0.528 0.528 FBXW2FBXW2 0.2860.286 CTBP1CTBP1 0.608 0.608 FBXW2FBXW2 0.3290.329 OAZ1 OAZ1 0.545 0.545 PAPOLAPAPOLA 0.2900.290 UBQLN1UBQLN1 0.623 0.623 DIMT1LDIMT1L 0.3640.364 UBQLN1 UBQLN1 0.555 0.555 TRIM27TRIM27 0.3270.327 DIMT1LDIMT1L 0.637 0.637 PPIAPPIA 0.3830.383 TRIM27 TRIM27 0.567 0.567 GPBP1GPBP1 0.3450.345 PPIAPPIA 0.661 0.661 UBQLN1UBQLN1 0.3980.398 TBP TBP 0.580 0.580 HPRT1HPRT1 0.3680.368 OAZ1OAZ1 0.682 0.682 OAZ1OAZ1 0.4380.438 CUL1CUL1 0.593 0.593 CUL1CUL1 0.3830.383 ZNF207ZNF207 0.709 0.709 ARL8BARL8B 0.4940.494 HPRT1 HPRT1 0.609 0.609 TBPTBP 0.4020.402 ARL8BARL8B 0.731 0.731 SPG21SPG21 0.5020.502 ZNF207 ZNF207 0.625 0.625 HMBSHMBS 0.4070.407 SPG21SPG21 0.749 0.749 ZNF207ZNF207 0.5020.502 HMBS HMBS 0.641 0.641 ZNF207ZNF207 0.4400.440 HPRT1HPRT1 0.770 0.770 HPRT1HPRT1 0.5160.516 GAPDH GAPDH 0.668 0.668 PPIAPPIA 0.4610.461 GAPDHGAPDH 0.793 0.793 HMBSHMBS 0.5870.587 PPIA PPIA 0.692 0.692 GAPDHGAPDH 0.5270.527 HMBSHMBS 0.815 0.815 GAPDHGAPDH 0.5910.591 B2M B2M 0.715 0.715 B2MB2M 0.5300.530 ACTBACTB 0.843 0.843 ACTBACTB 0.6180.618 ACTB ACTB 0.737 0.737 ACTBACTB 0.5410.541 B2MB2M 0.888 0.888 B2MB2M 0.8150.815 　　　　

*DIMT1L는 분석에서 제외되었다.* DIMT1L was excluded from the analysis.

M: geNorm 프로그램으로 계산된 평균 발현 안정성; 및,M: mean expression stability calculated by the geNorm program; And,

S: NormFinder 프로그램으로 계산된 안정성 값.S: Stability value calculated by the NormFinder program.

M: geNorm 프로그램으로 계산된 평균 발현 안정성; 및, M: mean expression stability calculated by the geNorm program; And,

S: NormFinder 프로그램으로 계산된 안정성 값. S: Stability value calculated by the NormFinder program.

HG-U133 어레이에서 13종의 조직을 포함하는 567 시료의 리스트List of 567 Samples Containing 13 Tissues from HG-U133 Array 조직group 카테고리category 형태학morphology 시료 갯수Number of samples BrainBrain BenignBenign MeningiomaMeningioma 77 2323 MalignantMalignant Glioblastoma MultiformeGlioblastoma Multiforme 77 OligodendrogliomaOligodendroglioma 66 MedulloblastomaMedulloblastoma 33 BreastBreast NormalNormal Normal TissueNormal Tissue 1818 7474 MalignantMalignant Infiltrating Duct CarcionmaInfiltrating Duct Carcionma 3636 Infiltrating Duct and Lobular Carcinoma Infiltrating Duct and Lobular Carcinoma 77 Infiltrating Lobular Carcinoma Infiltrating Lobular Carcinoma 1313 ColonColon NormalNormal Normal TissueNormal Tissue 2222 6262 MalignantMalignant AdenocarcinomaAdenocarcinoma 3333 Mucinous Adenocarcinoma Mucinous Adenocarcinoma 77 EsophagusEsophagus NormalNormal Normal TissueNormal Tissue 1111 1717 MalignantMalignant AdenocarcinomaAdenocarcinoma 66 KidneyKidney NormalNormal Normal TissueNormal Tissue 2626 5151 BenignBenign OncocytomaOncocytoma 55 MalignantMalignant Clear Cell AdenocarcinomaClear cell adenocarcinoma 66 Renal Cell Carcinoma Renal Cell Carcinoma 1414 LiverLiver NormalNormal Normal TissueNormal Tissue 1010 2929 MalignantMalignant Hepatocellular CarcinomaHepatocellular Carcinoma 1919 LungLung NormalNormal Normal TissueNormal Tissue 2626 5858 MalignantMalignant AdenocarcinomaAdenocarcinoma 1515 Squamous Cell Carcinoma Squamous Cell Carcinoma 1717 Lymph NodeLymph Node NormalNormal Normal TissueNormal Tissue 44 2323 MalignantMalignant Hodgkin's DiseaseHodgkin's disease 44 Malignant LymphomaMalignant Lymphoma 1515 OvaryOvary NormalNormal Normal TissueNormal Tissue 1010 5050 MalignantMalignant AdenocarcinomaAdenocarcinoma 55 Clear Cell AdenocarcinomaClear cell adenocarcinoma 66 Mucinous CystadenocarcinomaMucinous Cystadenocarcinoma 55 Serous Cystadenocarcinoma Serous Cystadenocarcinoma 66 Papillary Serous Adenocarcinoma Papillary Serous Adenocarcinoma 1818 PancreasPancreas NormalNormal Normal TissueNormal Tissue 1313 4141 MalignantMalignant AdenocarcinomaAdenocarcinoma 2828 ProstateProstate NormalNormal Normal TissueNormal Tissue 1414 4141 MalignantMalignant AdenocarcinomaAdenocarcinoma 2727 RectumRectum NormalNormal Normal TissueNormal Tissue 1717 3838 MalignantMalignant AdenocarcinomaAdenocarcinoma 2121 StomachStomach NormalNormal Normal TissueNormal Tissue 1717 6060 MalignantMalignant AdenocarcinomaAdenocarcinoma 3737 Signet Ring Cell CarcinomaSignet Ring Cell Carcinoma 66 총gun 567567

도 1은 내인성 표준 발현 유전자(Endogenous Reference Genes; ERG) 발굴 방법을 나타내는 모식도이다.1 is a schematic diagram showing a method for discovering endogenous reference genes (ERGs).

도 2는 FunCat(Functional Classification Catalogue)에 의해 분류된 후보ERG의 기능적 분포를 나타내는 그림이다:2 is a diagram showing the functional distribution of candidate ERGs classified by FunCat (Functional Classification Catalog):

숫자: 유전자의 개수; Number: number of genes;

A: 단백질 운명(접힘, 변형, 용도); A: protein fate (folding, modification, use);

B: 세포 이동, 이동 촉진 및 이동 경로; B: cell migration, migration promotion and migration pathway;

C: 전사; C: transcription;

D: 세포 정보교환/신호전달 체계; D: cell information exchange / signaling system;

E: 세포 주기 및 DNA 프로세싱; E: cell cycle and DNA processing;

F: 단백질 합성; F: protein synthesis;

G: 물질대사; G: metabolism;

H: 에너지; H: energy;

I: 세포 운명; I: cell fate;

J: 세포 환경의 상호작용; J: interaction of the cellular environment;

K: 환경의 상호작용(체계적); K: environmental interaction (systematic);

L: 기관 차이; L: organ difference;

M: 발달(체계적); M: developmental (systematic);

N: 단백질 활성 규제; N: protein activity regulation;

O: 조직 차이; O: tissue difference;

P: 세포 구성물의 생물발생설; P: biogenesis of cell constructs;

Q: 세포 구출, 방어 및 병독성; 및 Q: cell rescue, defense and virulence; And

R: 세포 형태 차이. R: Cell morphology difference.

도 3은 2,087개의 후보 ERG의 4개의 데이터세트[EST(expressed sequence tag), 짤막한 절편의 SAGE(serial analysis of gene expression short; ShortSAGE), 긴 절편의 SAGE(serial analysis of gene expression long; LongSAGE) 및 마이크로어레이 데이터세트] 사이의 유전자 발현량의 상관관계를 나타내는 그림이다.FIG. 3 shows four datasets (EST (expressed sequence tag) of 2,087 candidate ERGs, short segment SAGE (serial analysis of gene expression short; ShortSAGE), long segment SAGE (serial analysis of gene expression long; LongSAGE) and Microarray dataset].

도 4는 2,087개의 후보 ERG의 4개의 데이터세트(EST, ShortSAGE, LongSAGE 및 마이크로어레이 데이터세트) 사이의 변동계수(CV,%)의 상관관계를 나타내는 그림이다.FIG. 4 is a diagram showing a correlation of coefficients of variation (CV,%) between four data sets (EST, ShortSAGE, LongSAGE and microarray datasets) of 2,087 candidate ERGs.

도 5는 각 데이터세트에서의 본 발명에 의해 선별된 후보 ERG 및 비-후보 ERG 발현 비교를 나타내는 그래프이다:5 is a graph showing comparison of candidate ERG and non-candidate ERG expression selected by the present invention in each dataset:

상자그림(Box and Whisker plots): 자연 로그 값(ln)으로 표시된 발현값의 Box and Whisker plots: The expression of the expression in natural logarithms (ln)

분포; Distribution;

상자의 밑면: 전체 발현값 중 작은 순으로 25%에 해당하는 발현값; 및 Underside of the box: expression value corresponding to 25% in descending order of total expression values; And

상자의 윗면: 발현값 중 작은 순으로 75%에 해당하는 발현값. Top of box: 75% of the expression values, in descending order.

도 6은 본 발명에 의해 선별된 13개의 내인성 표준 발현 유전자(ERG)와 임의로 선택된 13개의 전통적 내인성 표준 발현 유전자 사이의 유전자 발현량의 비교를 나타내는 그래프이다.FIG. 6 is a graph showing the comparison of gene expression levels between 13 endogenous standard expression genes (ERG) selected by the present invention and 13 optionally selected endogenous standard expression genes.

도 7은 본 발명에 의해 선별된 13개의 내인성 표준 발현 유전자(ERG)와 임의로 선택된 13개의 전통적 내인성 표준 발현 유전자 사이의 CV(%) 비교도이다.FIG. 7 is a comparison of CV (%) between 13 endogenous standard expression genes (ERG) selected by the present invention and 13 optionally selected endogenous standard expression genes.

도 8은 실시간 PCR로 측정한 인체 동결 조직 및 암 세포주를 포함하는 48개 각 시료들에서의 신규 및 전통적 ERG의 mRNA 수준의 분포를 나타내는 그림이다.FIG. 8 is a diagram showing the distribution of mRNA levels of new and traditional ERGs in 48 individual samples including human frozen tissue and cancer cell lines measured by real time PCR.

도 9는 각 신규 및 전통적 ERG의 48개 시료(동결조직 및 암 세포 주 시료 포함)및 60개 FFPE(formalin-fixed paraffin-embedded) 조직 시료에서의 실시간 PCR로 측정된 Cp 값으로 표현된 mRNA 수준 범위를 나타내는 그래프이다. 상자그림의 가운데 선은 Cp값의 중간값을 의미하며 밑면은 Cp값의 작은 순으로 25%에 해당하는 값을, 윗면은 Cp값의 75%에 해당하는 값을 의미한다.Figure 9 mRNA levels expressed by Cp values measured by real-time PCR in 48 samples of each new and traditional ERG (including frozen tissue and cancer cell line samples) and 60 formalin-fixed paraffin-embedded tissue samples. Graph showing range. The middle line of the box figure represents the median of the Cp values, the bottom side represents 25% of the Cp values, and the top side represents 75% of the Cp values.

<110> Seoul National University <120> Data processing, analysis method of gene expression data to identify housekeeping Gene or endogenous reference genes <130> 8p-02-22 <160> 42 <170> KopatentIn 1.71 <210> 1 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> GAPDH F <400> 1 agccacatcg ctcagaca 18 <210> 2 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> GAPDH R <400> 2 gcccaatacg accaaatcc 19 <210> 3 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> ACTB F <400> 3 ccaaccgcga gaagatga 18 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> ACTB R <400> 4 ccagaggcgt acagggatag 20 <210> 5 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> B2M F <400> 5 ttctggcctg gaggctatc 19 <210> 6 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> B2M R <400> 6 tcaggaaatt tgactttcca ttc 23 <210> 7 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> PPIA F <400> 7 catctgcact gccaagactg ag 22 <210> 8 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> PPIA R <400> 8 tgcaatccag ctaggcatg 19 <210> 9 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> HPRT1 F <400> 9 tgaccttgat ttattttgca tacc 24 <210> 10 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> HPRT1 R <400> 10 cgagcaagac gttcagtcct 20 <210> 11 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> HMBS F <400> 11 tgtggtggga accagctc 18 <210> 12 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> HMBS R <400> 12 tgttgaggtt tccccgaat 19 <210> 13 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TBP F <400> 13 gctggcccat agtgatcttt 20 <210> 14 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> TBP R <400> 14 cttcacacgc caagaaacag t 21 <210> 15 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> H6PD F <400> 15 tggagatcat catgaaagag acc 23 <210> 16 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> H6PD R <400> 16 gcgaatgaca ccgtactcct 20 <210> 17 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> ZNF207 F <400> 17 ctgtttccta gcacagcaca a 21 <210> 18 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> ZNF207 R <400> 18 ggtttgaaat ctgtaccaac agg 23 <210> 19 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> OAZ1 F <400> 19 caccatgccg ctcctaag 18 <210> 20 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> OAZ1 R <400> 20 gagggagacc ctggaactct 20 <210> 21 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> LUC7L2 F <400> 21 cgatcacaca gcaagaatcc 20 <210> 22 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> LUC7L2 R <400> 22 agatcgatgt ctgcgatgc 19 <210> 23 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> CTBP1 F <400> 23 actgcgtgac cctgcact 18 <210> 24 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> CTBP1 R <400> 24 gccccttgtc tcatctgc 18 <210> 25 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> TRIM27 F <400> 25 caggcacgag ctgaactct 19 <210> 26 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TRIM27 R <400> 26 agctgctcaa actcccaaac 20 <210> 27 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> GPBP1 F <400> 27 tcacttgagg cagaacacag a 21 <210> 28 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> GPBP1 R <400> 28 agcacatgtt tcatcatttt cac 23 <210> 29 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> UBQLN1 F <400> 29 gaatcctgac cttgctgcac 20 <210> 30 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> UBQLN1 R <400> 30 ttgggagctg ttgtctcatt t 21 <210> 31 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> ARL8B F <400> 31 aagcatgtgg gagcggtat 19 <210> 32 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> ARL8B R <400> 32 cgatctgcag catctatcat gt 22 <210> 33 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> PAPOLA F <400> 33 gctacgaaga ccagtccatt g 21 <210> 34 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> PAPOLA R <400> 34 tgttggtcac agatgctgct 20 <210> 35 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> CUL1 F <400> 35 gcgaggtcct cactcagc 18 <210> 36 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> CUL1 R <400> 36 ttctttctca attagaatgt caatgc 26 <210> 37 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> DIMT1L F <400> 37 tccagtgttg taaggataga acctaag 27 <210> 38 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> DIMT1L R <400> 38 ccttactaga ccatcccatt cct 23 <210> 39 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> FBXW2 F <400> 39 cggctctgca gacttcact 19 <210> 40 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FBXW2 R <400> 40 ttgcacttct gcaaaactac ct 22 <210> 41 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SPG21 F <400> 41 gatgtctttt tccggcagat 20 <210> 42 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SPG21 R <400> 42 cgagatggtc ccaataaact g 21 <110> Seoul National University <120> Data processing, analysis method of gene expression data to identify housekeeping Gene or endogenous reference genes <130> 8p-02-22 <160> 42 <170> KopatentIn 1.71 <210> 1 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> GAPDH F <400> 1 agccacatcg ctcagaca 18 <210> 2 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> GAPDH R <400> 2 gcccaatacg accaaatcc 19 <210> 3 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> ACTB F <400> 3 ccaaccgcga gaagatga 18 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> ACTB R <400> 4 ccagaggcgt acagggatag 20 <210> 5 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> B2M F <400> 5 ttctggcctg gaggctatc 19 <210> 6 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> B2M R <400> 6 tcaggaaatt tgactttcca ttc 23 <210> 7 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> PPIA F <400> 7 catctgcact gccaagactg ag 22 <210> 8 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> PPIA R <400> 8 tgcaatccag ctaggcatg 19 <210> 9 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> HPRT1 F <400> 9 tgaccttgat ttattttgca tacc 24 <210> 10 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> HPRT1 R <400> 10 cgagcaagac gttcagtcct 20 <210> 11 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> HMBS F <400> 11 tgtggtggga accagctc 18 <210> 12 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> HMBS R <400> 12 tgttgaggtt tccccgaat 19 <210> 13 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TBP F <400> 13 gctggcccat agtgatcttt 20 <210> 14 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> TBP R <400> 14 cttcacacgc caagaaacag t 21 <210> 15 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> H6PD F <400> 15 tggagatcat catgaaagag acc 23 <210> 16 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> H6PD R <400> 16 gcgaatgaca ccgtactcct 20 <210> 17 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> ZNF207 F <400> 17 ctgtttccta gcacagcaca a 21 <210> 18 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> ZNF207 R <400> 18 ggtttgaaat ctgtaccaac agg 23 <210> 19 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> OAZ1 F <400> 19 caccatgccg ctcctaag 18 <210> 20 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> OAZ1 R <400> 20 gagggagacc ctggaactct 20 <210> 21 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> LUC7L2 F <400> 21 cgatcacaca gcaagaatcc 20 <210> 22 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> LUC7L2 R <400> 22 agatcgatgt ctgcgatgc 19 <210> 23 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> CTBP1 F <400> 23 actgcgtgac cctgcact 18 <210> 24 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> CTBP1 R <400> 24 gccccttgtc tcatctgc 18 <210> 25 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> TRIM27 F <400> 25 caggcacgag ctgaactct 19 <210> 26 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TRIM27 R <400> 26 agctgctcaa actcccaaac 20 <210> 27 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> GPBP1 F <400> 27 tcacttgagg cagaacacag a 21 <210> 28 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> GPBP1 R <400> 28 agcacatgtt tcatcatttt cac 23 <210> 29 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> UBQLN1 F <400> 29 gaatcctgac cttgctgcac 20 <210> 30 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> UBQLN1 R <400> 30 ttgggagctg ttgtctcatt t 21 <210> 31 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> ARL8B F <400> 31 aagcatgtgg gagcggtat 19 <210> 32 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> ARL8B R <400> 32 cgatctgcag catctatcat gt 22 <210> 33 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> PAPOLA F <400> 33 gctacgaaga ccagtccatt g 21 <210> 34 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> PAPOLA R <400> 34 tgttggtcac agatgctgct 20 <210> 35 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> CUL1 F <400> 35 gcgaggtcct cactcagc 18 <210> 36 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> CUL1 R <400> 36 ttctttctca attagaatgt caatgc 26 <210> 37 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> DIMT1L F <400> 37 tccagtgttg taaggataga acctaag 27 <210> 38 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> DIMT1L R <400> 38 ccttactaga ccatcccatt cct 23 <210> 39 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> FBXW2 F <400> 39 cggctctgca gacttcact 19 <210> 40 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FBXW2 R <400> 40 ttgcacttct gcaaaactac ct 22 <210> 41 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SPG21 F <400> 41 gatgtctttt tccggcagat 20 <210> 42 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SPG21 R <400> 42 cgagatggtc ccaataaact g 21

Claims

1) quantifying the expression levels of genes in the EST, SAGE and microarray data sets; And,

2) A method for screening candidate endogenous reference genes (ERGs) comprising selecting a gene that is always expressed in various tissues using the gene expression amount and zero ratio of step 1).

The method of claim 1, wherein the expression level of the gene expression in step 1) is characterized by using the following equations (1) and (2).

.

The method of claim 1, wherein the data set of step 1) expresses mRNAs derived from CGAP EST (Hs_ExprData.dat), SAGE (Hs_short.frequencies.gz and Hs_long.frequencies.gz) and microarray (Affymetrix HG-U133, CA). A method comprising the numerical value data.

The method of claim 1, wherein the zero ratio of step 2) is described by Equation 3 below.

.

A composition for detection of the candidate standard expression gene comprising a detection reagent capable of detecting the candidate standard expression gene selected by the method of claim 1.

The method of claim 5, wherein the selected candidate standard expression gene is Accession No. 6. Hs 120 (PRDX6), Accession No. Hs 142 (SULT1A1), Accession No. Hs 202 (BZRP), Accession No. Hs 429 (ATP5G3), Accession No. Hs 695 (CSTB), Accession No. Hs 808 (HNRPF), Accession No. Hs 861 (MAPK3), Accession No. Hs 1063 (SNRPC), Accession No. Hs 1103 (TGFB1), Accession No. Hs 2430 (TCFL1), Accession No. Hs 2533 (ALDH9A1), Accession No. Hs 2795 (LDHA), Accession No. Hs 2853 (PCBP1), Accession No. Hs 3100 (KARS), Accession No. Hs 3254 (MRPL23), Accession No. Hs 3353 (G3BP), Accession No. Hs 3416 (ADFP), Accession No. Hs 439 (STOML2), Accession No. Hs 3530 (FUSIP1), Accession No. Hs 3989 (PLXNB2), Accession No. Hs 4055 (KLF6), Accession No. Hs 4742 (GPAA1), Accession No. Hs 4747 (DKC1), Accession No. Hs 4766 (FAM32A), Accession No. Hs 4859 (CCNL1), Accession No. Hs 4997 (RBM23), Accession No. Hs 4998 (TMOD3), Accession No. Hs 5062 (TM4SF8), Accession No. Hs 5086 (MGC10433), Accession No. Hs 5120 (DNCL1), Accession No. Hs 5158 (ILK), Accession No. Hs 5245 (FLJ20643), Accession No. Hs 5258 (MAGED1), Accession No. Hs 5268 (ZDHHC4), Accession No. Hs 5298 (ADIPOR1), Accession No. Hs 5308 (UBA52), Accession No. Hs 5324 (C2orf25), Accession No. Hs 5345 (RNPEPL1), Accession No. Hs 5662 (GNB2L1), Accession No. Hs 5710 (CREG1), Accession No. Hs 5719 (CNAP1), Accession No. Hs 5912 (FBXO7), Accession No. Hs 5947 (RAB8A), Accession No. Hs 6396 (JTB), Accession No. Hs 6454 (RGS19IP1), Accession No. Hs 6459 (GPR172A), Accession No. Hs 6551 (ATP6AP1), Accession No. Hs 6891 (SFRS6), Accession No. Hs 7101 (ANAPC5), Accession No. Hs 7236 (NOSIP), Accession No. Hs 7476 (ATP6V0B), Accession No. Hs 7527 (DKFZP566E144), Accession No. Hs 7744 (NDUFV1), Accession No. Hs 7753 (CALU), Accession No. Hs 7768 (FIBP), Accession No. Hs 7862 (PNRC2), Accession No. Hs 7910 (RYBP), Accession No. Hs 7917 (HIG1), Accession No. Hs 8102 (RPS20), Accession No. Hs 8372 (UQCR), Accession No. Hs 8737 (WDR6), Accession No. Hs 8752 (TMEM4), Accession No. Hs 8765 (DDX42), Accession No. Hs 8859 (CANT1), Accession No. Hs 8867 (CYR61), Accession No. Hs 9003 (FLJ13868), Accession No. Hs 9015 (MGC52000), Accession No. Hs 9043 (C14orf120), Accession No. Hs 9234 (NIFIE14), Accession No. Hs 9235 (NME4), Accession No. Hs 9527 (C2orf28), Accession No. Hs 9534 (SEC11L1), Accession No. Hs 9573 (ABCF1), Accession No. Hs 9589 (UBQLN1), Accession No. Hs 9788 (NDFIP1), Accession No. Hs 9825 (CGI-128), Accession No. Hs 9857 (DCXR), Accession No. Hs 10326 (COPE), Accession No. Hs 10842 (RAN), Accession No. Hs 10848 (BMS1L), Accession No. Hs 11125 (SPCS1), Accession No. Hs 11184 (UBE2R2), Accession No. Hs 11223 (IDH1), Accession No. Hs 11355 (TMPO), Accession No. Hs 11463 (UMP-CMPK), Accession No. Hs 12013 (ABCE1), Accession No. Hs 12084 (TUFM), Accession No. Hs 12102 (SNX3), Accession No. Hs 12107 (BC-2), Accession No. Hs 12109 (WDR39), Accession No. Hs 12144 (KIAA1033), Accession No. Hs 12152 (SRPRB), Accession No. Hs 12272 (BECN1), Accession No. Hs 12341 (ADAR), Accession No. Hs 12457 (NUP133), Accession No. Hs 12865 (NSFL1C), Accession No. Hs 13662 (MGC5508), Accession No. Hs 14317 (NOLA3), Accession No. Hs 14333 (FLJ10349), Accession No. Hs 14745 (C10orf9), Accession No. Hs 14839 (POLR2G), Accession No. Hs 14846 (SLC7A1), Accession No. Hs 14894 (TGOLN2), Accession No. Hs 15277 (C16orf33), Accession No. Hs 15591 (COPS6), Accession No. Hs 15738 (RAB7), Accession No. Hs 16059 (HSPC009), Accession No. Hs 16130 (E2-230K), Accession No. Hs 16349 (KIAA0431), Accession No. Hs 17118 (FLJ11730), Accession No. Hs 17250 (MGC4767), Accession No. Hs 17680 (FUCA2), Accession No. Hs 17731 (FLJ12892), Accession No. Hs 17883 (PPM1G), Accession No. Hs 18069 (LGMN), Accession No. Hs 18128 (C20orf44), Accession No. Hs 18349 (MRPL15), Accession No. Hs 19673 (MAF1), Accession No. Hs 20013 (P29), Accession No. Hs 20107 (KNS2), Accession No. Hs 20157 (CDK5RAP3), Accession No. Hs 20521 (HRMT1L2), Accession No. Hs 20529 (LOC127262), Accession No. Hs 20573 (IGF1R), Accession No. Hs 20716 (TIMM17A), Accession No. Hs 22393 (DENR), Accession No. Hs 22543 (UBE3A), Accession No. Hs 22546 (CYBASC3), Accession No. Hs 22616 (KIAA0664), Accession No. Hs 23033 (LOC92912), Accession No. Hs 23111 (FARSLA), Accession No. Hs 23978 (SAFB), Accession No. Hs 24301 (POLR2E), Accession No. Hs 24379 (TRAPPC1), Accession No. Hs 24601 (FBLN1), Accession No. Hs 24950 (RGS5), Accession No. Hs 25155 (NET1), Accession No. Hs 25450 (SLC29A1), Accession No. Hs 25723 (MTVR1), Accession No. Hs 26010 (PFKP), Accession No. Hs 26023 (FOXJ3), Accession No. Hs 26136 (MGC14156), Accession No. Hs 26232 (MAN2C1), Accession No. Hs 26403 (GSTZ1), Accession No. Hs 26518 (TM4SF7), Accession No. Hs 27222 (NOLA2), Accession No. Hs 28491 (SAT), Accession No. Hs 28914 (APRT), Accession No. Hs 29203 (GBL), Accession No. Hs 29665 (CLSTN1), Accession No. Hs 30011 (MGC2963), Accession No. Hs 30026 (HSPC182), Accession No. Hs 30345 (TRAP1), Accession No. Hs 30954 (PMVK), Accession No. Hs 31053 (CKAP1), Accession No. Hs 31334 (C20orf14), Accession No. Hs 31387 (DKFZP564J0123), Accession No. Hs 34045 (CDCA4), Accession No. Hs 34576 (TAX1BP1), Accession No. Hs 34906 (BLOC1S2), Accession No. Hs 35052 (TEGT), Accession No. Hs 35828 (MARK3), Accession No. Hs 36587 (PPP1R7), Accession No. Hs 36927 (HSPH1), Accession No. Hs 37616 (STRA13), Accession No. Hs 37916 (DPP7), Accession No. Hs 42806 (Cab45), Accession No. Hs 43297 (MTPN), Accession No. Hs 47062 (POLR2I), Accession No. Hs 50098 (NDUFA4), Accession No. Hs 50308 (HIP2), Accession No. Hs 50425 (TEBP), Accession No. Hs 53066 (HSPBP1), Accession No. Hs 54277 (FAM50A), Accession No. Hs 54457 (CD81), Accession No. Hs 54642 (MAT2B), Accession No. Hs 54649 (RY1), Accession No. Hs 55682 (EIF3S7), Accession No. Hs 55847 (MRPL51), Accession No. Hs 58488 (CTNNAL1), Accession No. Hs 58992 (SMC4L1), Accession No. Hs 59486 (HSDL2), Accession No. Hs 61812 (PTPN12), Accession No. Hs 65234 (DDX27), Accession No. Hs 65238 (RNF40), Accession No. Hs 66048 (BPY2IP1), Accession No. Hs 66915 (C22orf16), Accession No. Hs 68714 (SFRS1), Accession No. Hs 9293 (HEXB), Accession No. Hs 69554 (RNF126), Accession No. Hs 69855 (UNR), Accession No. Hs 71465 (SQLE), Accession No. Hs 71787 (MRPS7), Accession No. Hs 73527 (CSNK2B), Accession No. Hs 73722 (APEX1), Accession No. Hs 73799 (GNAI3), Accession No. Hs 73965 (SFRS2), Accession No. Hs 74047 (ETFB), Accession No. Hs 74050 (FVT1), Accession No. Hs 74137 (TMP21), Accession No. Hs 74375 (DVL1), Accession No. Hs 74405 (YWHAQ), Accession No. Hs 74471 (GJA1), Accession No. Hs 74563 (OAZ2), Accession No. Hs 74564 (SSR2), Accession No. Hs 74576 (GDI1), Accession No. Hs 75056 (TIMM13), Accession No. Hs 75061 (MARCKSL1), Accession No. Hs 75066 (TSN), Accession No. Hs 75087 (FASTK), Accession No. Hs 75117 (ILF2), Accession No. Hs 75133 (TFAM), Accession No. Hs 75139 (ARFIP2), Accession No. Hs 75189 (DAP), Accession No. Hs 75227 (NDUFA9), Accession No. Hs 75243 (BRD2), Accession No. Hs 75249 (ARL6IP), Accession No. Hs 75254 (IRF3), Accession No. Hs 75318 (TUBA1), Accession No. Hs 75348 (PSME1), Accession No. Hs 75438 (QDPR), Accession No. Hs 75527 (ADSL), Accession No. Hs 75724 (COPB2), Accession No. Hs 75798 (C20orf111), Accession No. Hs 75841 (C12orf8), Accession No. Hs 75890 (MBTPS1), Accession No. Hs 75914 (RNP24), Accession No. Hs 76111 (DAG1), Accession No. Hs 76394 (ECHS1), Accession No. Hs 76480 (UBL4), Accession No. Hs 76662 (ZDHHC16), Accession No. Hs 76686 (GPX1), Accession No. Hs 76847 (GANAB), Accession No. Hs 77060 (PSMB6), Accession No. Hs 77269 (GNAI2), Accession No. Hs 77313 (CDK10), Accession No. Hs 77422 (PLP2), Accession No. Hs 77558 (HMGN3), Accession No. Hs 77578 (USP9X), Accession No. Hs 77793 (CSK), Accession No. Hs 77897 (SF3A3), Accession No. Hs 77961 (HLA-B), Accession No. Hs 77978 (DKFZp761I2123), Accession No. Hs 78466 (PSMD8), Accession No. Hs 78601 (UROD), Accession No. Hs 78771 (PGK1), Accession No. Hs 78880 (ILVBL), Accession No. Hs 78888 (DBI), Accession No. Hs 78989 (ADH5), Accession No. Hs 79064 (DHPS), Accession No. Hs 79081 (PPP1CC), Accession No. Hs 79088 (RCN2), Accession No. Hs 79101 (CCNG1), Accession No. Hs 79110 (NCL), Accession No. Hs 79322 (QARS), Accession No. Hs 79335 (SMARCD1), Accession No. Hs 79387 (PSMC5), Accession No. Hs 79402 (POLR2C), Accession No. Hs 79411 (RPA2), Accession No. Hs 79625 (C20orf149), Accession No. Hs 80545 (RPL37), Accession No. Hs 80919 (SYPL), Accession No. Hs 80986 (ATP5G1), Accession No. Hs 81328 (NFKBIA), Accession No. Hs 81424 (SUMO1), Accession No. Hs 81848 (RAD21), Accession No. Hs 81964 (SEC24C), Accession No. Hs 82201 (CSNK2A2), Accession No. Hs 82327 (GSS), Accession No. Hs 82719 (MGC21416), Accession No. Hs 82793 (PSMB3), Accession No. Hs 82887 (PPP1R11), Accession No. Hs 82890 (DAD1), Accession No. Hs 82916 (CCT6A), Accession No. Hs 82927 (AMPD2), Accession No. Hs 83190 (FASN), Accession No. Hs 83347 (AAMP), Accession No. Hs 83383 (PRDX4), Accession No. Hs 83734 (STX4A), Accession No. Hs 83753 (SNRPB), Accession No. Hs 83765 (DHFR), Accession No. Hs 83916 (NDUFA5), Accession No. Hs 84359 (GABARAP), Accession No. Hs 84753 (FLJ12442), Accession No. Hs 85155 (ZFP36L1), Accession No. Hs 85769 (ERBP), Accession No. Hs 85962 (DERPC), Accession No. Hs 86131 (FADD), Accession No. Hs 87752 (MSN), Accession No. Hs 89545 (PSMB4), Accession No. Hs 89643 (TKT), Accession No. Hs 89649 (EPHX1), Accession No. Hs 89781 (UBTF), Accession No. Hs 89864 (SKIV2L), Accession No. Hs 90061 (PGRMC1), Accession No. Hs 90093 (HSPA4), Accession No. Hs 90107 (ADRM1), Accession No. Hs 90443 (NDUFS8), Accession No. Hs 91142 (KHSRP), Accession No. Hs 91531 (MLLT6), Accession No. Hs 93659 (ERP70), Accession No. Hs 93832 (LOC54499), Accession No. Hs 95577 (CDK4), Accession No. Hs 96530 (COX11), Accession No. Hs 96852 (FLJ21128), Accession No. Hs 96996 (HNRPA0), Accession No. Hs 97616 (SH3GL1), Accession No. Hs 97887 (RCN1), Accession No. Hs 98751 (FUBP3), Accession No. Hs 98791 (ACTR1B), Accession No. Hs 102696 (MCTS1), Accession No. Hs 102798 (PSMA1), Accession No. Hs 103561 (ARL6IP4), Accession No. Hs 103834 (MGC5576), Accession No. Hs 104839 (TIMP2), Accession No. Hs 105547 (NPDC1), Accession No. Hs 106185 (RALGDS), Accession No. Hs 106876 (ATP6V0D1), Accession No. Hs 106909 (ANAPC13), Accession No. Hs 107003 (CCNB1IP1), Accession No. Hs 107101 (FLJ31031), Accession No. Hs 107387 (C7orf20), Accession No. Hs 107393 (C3orf4), Accession No. Hs 108029 (SH3BGRL), Accession No. Hs 108080 (CSRP1), Accession No. Hs 108371 (E2F4), Accession No. Hs 108408 (APH-1A), Accession No. Hs 108957 (RPS27L), Accession No. Hs 108969 (PTD008), Accession No. Hs 109051 (SH3BGRL3), Accession No. Hs 109052 (C14orf2), Accession No. Hs 109672 (SIAT7F), Accession No. Hs 109798 (C6orf48), Accession No. Hs 110695 (SF3B5), Accession No. Hs 110849 (ESRRA), Accession No. Hs 111286 (MRPS11), Accession No. Hs 111577 (ITM2C), Accession No. Hs 111801 (ARS2), Accession No. Hs 112058 (SIVA), Accession No. Hs 112318 (TOMM7), Accession No. Hs 112955 (NUDT5), Accession No. Hs 114033 (SSR1), Accession No. Hs 114286 (CD9), Accession No. Hs 114412 (TXNL1), Accession No. Hs 115474 (RFC3), Accession No. Hs 115792 (EXOSC7), Accession No. Hs 116448 (GLS), Accession No. Hs 117176 (PABPN1), Accession No. Hs 117715 (ST5), Accession No. Hs 118110 (BST2), Accession No. Hs 118400 (FSCN1), Accession No. Hs 118463 (PNPLA2), Accession No. Hs 118638 (NME1), Accession No. Hs 118722 (FUT8), Accession No. Hs 118964 (p66alpha), Accession No. Hs 118983 (GSDMDC1), Accession No. Hs 19177 (ARF3), Accession No. Hs 119192 (H2AFZ), Accession No. Hs 119251 (UQCRC1), Accession No. Hs 119591 (AP2S1), Accession No. Hs 119598 (RPL3), Accession No. Hs 120323 (DNAPTP6), Accession No. Hs 121088 (NUP153), Accession No. Hs 121549 (CDIPT), Accession No. Hs 122363 (WIPI-2), Accession No. Hs 122523 (SND1), Accession No. Hs 124126 (ARPC1A), Accession No. Hs 124147 (FBXL11), Accession No. Hs 124246 (C10orf119), Accession No. Hs 124366 (BBX), Accession No. Hs 125113 (CCT8), Accession No. Hs 125867 (EVL), Accession No. Hs 125898 (GNAS), Accession No. Hs 126497 (AEBP2), Accession No. Hs 126774 (RAMP), Accession No. Hs 126938 (NAPA), Accession No. Hs 127092 (DHX38), Accession No. Hs 127249 (EAP30), Accession No. Hs 127386 (MAMDC2), Accession No. Hs 127764 (RAB5C), Accession No. Hs 128065 (CTSC), Accession No. Hs 128199 (SEPT11), Accession No. Hs 128548 (WDR1), Accession No. Hs 129634 (CINP), Accession No. Hs 129673 (EIF4A1), Accession No. Hs 130031 (TRIO), Accession No. Hs 130098 (DDX23), Accession No. Hs 130293 (CROP), Accession No. Hs 130413 (TM9SF2), Accession No. Hs 131226 (BNIP3L), Accession No. Hs 132497 (PRNPIP), Accession No. Hs 132513 HSD17B12), Accession No. Hs 133892 (TPM1), Accession No. Hs 134074 (SLC35E1), Accession No. Hs 134688 (PSMD13), Accession No. Hs 135406 (CEBPZ), Accession No. Hs 136905 (UREB1), Accession No. Hs 136947 (RALY), Accession No. Hs 137510 (NCOR2), Accession No. Hs 138860 (ARHGAP1), Accession No. Hs 139896 (MAEA), Accession No. Hs 140452 (M6PRBP1), Accession No. Hs 142442 (HP1-BP74), Accession No. Hs 143187 (DDX49), Accession No. Hs 143766 (DRPLA), Accession No. Hs 143873 (S100A10), Accession No. Hs 144058 (EBSP), Accession No. Hs 144468 (MGC3234), Accession No. Hs 144835 (EEF1G), Accession No. Hs 144868 (VTI1B), Accession No. Hs 144941 (MUF1), Accession No. Hs 144949 (ZNF313), Accession No. Hs 144980 (SCAMP4), Accession No. Hs 145049 (PLEKHM2), Accession No. Hs 145442 (MAP2K1), Accession No. Hs 145575 (UBL3), Accession No. Hs 146070 (TPM3), Accession No. Hs 146393 (HERPUD1), Accession No. Hs 146602 (QP-C), Accession No. Hs 146804 (SPIN), Accession No. Hs 146806 (CUL1), Accession No. Hs 147433 (PCNA), Accession No. Hs 148078 (RBAF600), Accession No. Hs 148272 (CCM2), Accession No. Hs 148330 (ARF4), Accession No. Hs 148340 (PTPRG), Accession No. Hs 148670 (RHOBTB1), Accession No. Hs 149004 (FBXO31), Accession No. Hs 149957 (RPS6KA1), Accession No. Hs 149983 (PEX14), Accession No. Hs 150107 (BIRC6), Accession No. Hs 150540 (BC002942), Accession No. Hs 150580 (SUI1), Accession No. Hs 150837 (TXNDC5), Accession No. Hs 151134 (OXA1L), Accession No. Hs 151220 (KIAA0992), Accession No. Hs 151413 (GMFB), Accession No. Hs 151787 (U5-116KD), Accession No. Hs 152536 (p44S10), Accession No. Hs 153177 (RPS28), Accession No. Hs 154023 (TXNDC4), Accession No. Hs 154073 (SLC35B1), Accession No. Hs 155165 (ZFPL1), Accession No. Hs 155218 (HNRPUL1), Accession No. Hs 155396 (NFE2L2), Accession No. Hs 155829 (KIAA0676), Accession No. Hs 156171 (PSMC6), Accession No. Hs 156367 (RPS29), Accession No. Hs 156667 (KIAA1536), Accession No. Hs 157160 (MRPS34), Accession No. Hs 157351 (PTD004), Accession No. Hs 157379 (H2AFV), Accession No. Hs 157394 (HAGH), Accession No. Hs 159014 (PRPF4B), Accession No. Hs 159118 (AMD1), Accession No. Hs 159130 (RAF1), Accession No. Hs 159161 (ARHGDIA), Accession No. Hs 159699 (FBXO21), Accession No. Hs 159799 (THRAP2), Accession No. Hs 160958 (CDC37), Accession No. Hs 161357 (PDHB), Accession No. Hs 162032 (HBP1), Accession No. Hs 162233 (CHD4), Accession No. Hs 162877 (PACSIN2), Accession No. Hs 163645 (MOCS2), Accession No. Hs 163776 (UBE2J1), Accession No. Hs 163893 (PICALM), Accession No. Hs 165195 (VAPA), Accession No. Hs 166011 (CTNND1), Accession No. Hs 166204 (PHF1), Accession No. Hs 166463 (HNRPU), Accession No. Hs 166924 (SEC13L1), Accession No. Hs 166975 (SFRS5), Accession No. Hs 167535 (SRP54), Accession No. Hs 168073 (TRPC4AP), Accession No. Hs 168799 (METTL3), Accession No. Hs 169611 (DIABLO), Accession No. Hs 169718 (CNN2), Accession No. Hs 170107 (UQCRFS1), Accession No. Hs 170131 (NFIC), Accession No. Hs 170553 (CNOT7), Accession No. Hs 170622 (CFL1), Accession No. Hs 171626 (SKP1A), Accession No. Hs 172550 (PTBP1), Accession No. Hs 172755 (BRP44L), Accession No. Hs 172928 (COL1A1), Accession No. Hs 173024 (NYREN18), Accession No. Hs 173162 (NOC4), Accession No. Hs 173381 (DPYSL2), Accession No. Hs 173464 (FKBP8), Accession No. Hs 173611 (NDUFS2), Accession No. Hs 173705 (LOC401152), Accession No. Hs 173724 (CKB), Accession No. Hs 174050 (EDF1), Accession No. Hs 174195 (IFITM2), Accession No. Hs 175473 (AK1), Accession No. Hs 175955 (YT521), Accession No. Hs 177530 (ATP5E), Accession No. Hs 177766 (PARP1), Accession No. Hs 178551 (RPL8), Accession No. Hs 178728 (MBD3), Accession No. Hs 179986 (FLOT1), Accession No. Hs 180141 (CFL2), Accession No. Hs 180312 (MRPS16), Accession No. Hs 180414 (HSPA8), Accession No. Hs 180877 (H3F3B), Accession No. Hs 180903 (384D8-2), Accession No. Hs 180909 (PRDX1), Accession No. Hs 180933 (CXXC1), Accession No. Hs 181046 (DUSP3), Accession No. Hs 181112 (MED4), Accession No. Hs 181163 (HMGN2), Accession No. Hs 181244 (HLA-A), Accession No. Hs 181368 (PRPF8), Accession No. Hs 181444 (TMEM9), Accession No. Hs 182255 (NHP2L1), Accession No. Hs 182626 (C22orf5), Accession No. Hs 182885 (SLC35B2), Accession No. Hs 183684 (EIF4G2), Accession No. Hs 183706 (ADD1), Accession No. Hs 183800 (RANGAP1), Accession No. Hs 183850 (DCTD), Accession No. Hs 183994 (PPP1CA), Accession No. Hs 184062 (C20orf24), Accession No. Hs 184211 (PMPCB), Accession No. Hs 184233 (HSPA9B), Accession No. Hs 184492 (ELAVL1), Accession No. Hs 185172 (GNB2), Accession No. Hs 185597 (SPG7), Accession No. Hs 187199 (MALAT1), Accession No. Hs 187635 (RPS15A), Accession No. Hs 187763 (BRD4), Accession No. Hs 187866 (SDFR1), Accession No. Hs 187946 (SLC20A1), Accession No. Hs 188501 (PAFAH1B2), Accession No. Hs 188614 (PLEKHA5), Accession No. Hs 188879 (RBM6), Accession No. Hs 188882 (NUDT3), Accession No. Hs 189075 (PTK9), Accession No. Hs 189119 (CXXC5), Accession No. Hs 189329 (SMURF1), Accession No. Hs 189716 (NDUFAB1), Accession No. Hs 189772 (CCT2), Accession No. Hs 190028 (GSTO1), Accession No. Hs 190086 (MRCL3), Accession No. Hs 190334 (RAP1A), Accession No. Hs 190384 (COPS4), Accession No. Hs 190722 (HSPC142), Accession No. Hs 190904 (STRN4), Accession No. Hs 191186 (TTC17), Accession No. Hs 191346 (SEPT7), Accession No. Hs 191518 (DHX9), Accession No. Hs 191987 (UBE2J2), Accession No. Hs 192316 (CDC2L1), Accession No. Hs 192374 (TRA1), Accession No. Hs 192425 (EIF3S8), Accession No. Hs 193118 (RAI17), Accession No. Hs 193163 (BIN1), Accession No. Hs 193491 (TUBB6), Accession No. Hs 194329 (TCEAL4), Accession No. Hs 194718 (ZNF265), Accession No. Hs 195464 (FLNA), Accession No. Hs 195642 (C17orf27), Accession No. Hs 196983 (SSFA2), Accession No. Hs 198281 (PKM2), Accession No. Hs 199561 (RANBP2), Accession No. Hs 199625 (HAX1), Accession No. Hs 200063 (HDAC7A), Accession No. Hs 200600 (SCAMP3), Accession No. Hs 200804 (SDCBP), Accession No. Hs 201253 (ch-TOG), Accession No. Hs 201390 (WDR45L), Accession No. Hs 201712 (GLG1), Accession No. Hs 202011 (GK001), Accession No. Hs 202085 (VDAC1), Accession No. Hs 202166 (HNRPH1), Accession No. Hs 202179 (SMN2), Accession No. Hs 203099 (KIAA0261), Accession No. Hs 203910 (SGTA), Accession No. Hs 204041 (AHSA1), Accession No. Hs 204773 (MEP50), Accession No. Hs 205 163 (MRPL3), Accession No. Hs 206 500 (CTTN), Accession No. Hs 206824 (MGC71993), Accession No. Hs 208597 (CTBP1), Accession No. Hs 209983 (STMN1), Accession No. Hs 210469 (ELMO2), Accession No. Hs 210532 (KIAA0141), Accession No. Hs 211463 (DNM2), Accession No. Hs 211594 (PSMC4), Accession No. Hs 211914 (NDUFS7), Accession No. Hs 212102 (TXNDC7), Accession No. Hs 212395 (CIZ1), Accession No. Hs 213061 (NUCKS), Accession No. Hs 213470 (PSMB7), Accession No. Hs 213541, Accession No. Hs 213666 (KIAA0460), Accession No. Hs 213724 (SUPT16H), Accession No. Hs 216653 (FBXO9), Accession No. Hs 220950 (FOXO3A), Accession No. Hs 221847 (SLC38A2), Accession No. Hs 222510 (DAZAP1), Accession No. Hs 223141 (DDX21), Accession No. Hs 224607 (SDC1), Accession No. Hs 226007 (RDH11), Accession No. Hs 226117 (H1F0), Accession No. Hs 226755 (YWHAH), Accession No. Hs 227067 (ATAD3A), Accession No. Hs 227253 (TOMM70A), Accession No. Hs 227777 (PTP4A1), Accession No. Hs 229641 (PC4), Accession No. Hs 231295 (PITPNC1), Accession No. Hs 231616 (HSPC023), Accession No. Hs 232194 (KIAA0174), Accession No. Hs 232543 (PDCD4), Accession No. Hs 233458 (NFYC), Accession No. Hs 233552 (CDC2L5), Accession No. Hs 233952 (PSMA7), Accession No. Hs 234521 (MAPKAPK3), Accession No. Hs 236030 (SMARCC2), Accession No. Hs 237536 (MGC20781), Accession No. Hs 237971 (XTP3TPA), Accession No. Hs 238839 (SCYL1), Accession No. Hs 240170 (MGC2731), Accession No. Hs 241336 (ATPIF1), Accession No. Hs 241543 (POLDIP2), Accession No. Hs 241558 (ARIH2), Accession No. Hs 241575 (GNPTG), Accession No. Hs 241576 (DERL1), Accession No. Hs 241579 (SERPINH1), Accession No. Hs 242458 (SPG21), Accession No. Hs 242947 (DGKI), Accession No. Hs 246112 (ASCC3L1), Accession No. Hs 246310 (ATP5J), Accession No. Hs 246413 (CPNE1), Accession No. Hs 246781 (FBXO11), Accession No. Hs 247077 (RHOA), Accession No. Hs 247186 (FBS1), Accession No. Hs 247975 (HSPD1), Accession No. Hs 248267 (MPST), Accession No. Hs 248941 (TAF9), Accession No. Hs 49600 (DLGAP4), Accession No. Hs 250009 (ARL10C), Accession No. Hs 250429 (SUPT6H), Accession No. Hs 250758 (PSMC3), Accession No. Hs 250899 (HSBP1), Accession No. Hs 250905 (LOC51234), Accession No. Hs 251531 (PSMA4), Accession No. Hs 252457 (MVD), Accession No. Hs 252713 (TTC15), Accession No. Hs 252967 DKFZp566C0424), Accession No. Hs 253726 (PAPOLA), Accession No. Hs 253903 (STOM), Accession No. Hs 254042 (BAT1), Accession No. Hs 255015 (VPS24), Accession No. Hs 255093 (PFKL), Accession No. Hs 255932 (XRN2), Accession No. Hs 255935 (BTG1), Accession No. Hs 255973 (CRI1), Accession No. Hs 256301 (MGC13170), Accession No. Hs 256549 (NUBP2), Accession No. Hs 257008 (PLD3), Accession No. Hs 257341 (SAV1), Accession No. Hs 57761 (SH3BP5), Accession No. Hs 258551 (DNPEP), Accession No. Hs 258563 (FEZ2), Accession No. Hs 258798 C10orf86), Accession No. Hs 259461 (PALM2-AKAP2), Accession No. Hs 260603 (PIP5K2B), Accession No. Hs 262823 (FLJ10326), Accession No. Hs 265829 (ITGA3), Accession No. Hs 268488 (KIAA1185), Accession No. Hs 268530 (GPS1), Accession No. Hs 268742 (C13orf12), Accession No. Hs 268849 (GLO1), Accession No. Hs 268939 (MATR3), Accession No. Hs 269528 (MAK3), Accession No. Hs 269577 (PTPRA), Accession No. Hs 269782 (GNAQ), Accession No. Hs 269944 (MTCH2), Accession No. Hs 270291 (ACTN4), Accession No. Hs 270428 (SUCLG1), Accession No. Hs 270525 (LASS5), Accession No. Hs 270869 (ZNF410), Accession No. Hs 271135 (ATP5C1), Accession No. Hs 271695 (NOB1P), Accession No. Hs 272062 (PTPRF), Accession No. Hs 272168 (TDE1), Accession No. Hs 272630 (ATP6V1D), Accession No. Hs 272927 (SEC23A), Accession No. Hs 273077 (TMEM14B), Accession No. Hs 274184 (TFE3), Accession No. Hs 274772 (C15orf15), Accession No. Hs 274873 (CARS), Accession No. Hs 275243 (S100A6), Accession No. Hs 275775 (SEPP1), Accession No. Hs 275865 (PCNP), Accession No. Hs 276878 (NUP93), Accession No. Hs 277035 (MGLL), Accession No. Hs 277517 (C11orf2), Accession No. Hs 278186 (ARHGEF1), Accession No. Hs 278362 (MEA), Accession No. Hs 278426 (PDAP1), Accession No. Hs 278429 (C9orf78), Accession No. Hs 278500 (GNPDA1), Accession No. Hs 278569 (SNX17), Accession No. Hs 278573 (CD59), Accession No. Hs 278721 (SLC39A7), Accession No. Hs 279061 (C17orf25), Accession No. Hs 279245 (TACC1), Accession No. Hs 279257 (PCMT1), Accession No. Hs 279413 (POLD1), Accession No. Hs 279529 (PX19), Accession No. Hs 279583 (DREV1), Accession No. Hs 279623 (SEPX1), Accession No. Hs 279640 (TPR), Accession No. Hs 279652 (MRPL4), Accession No. Hs 279669 (TUBG1), Accession No. Hs 279696 (SUMF2), Accession No. Hs 279806 (DDX5), Accession No. Hs 79836 (COMMD9), Accession No. Hs 279920 (YWHAB), Accession No. Hs 279929 (TMED9), Accession No. Hs 80202 (SBF1), Accession No. Hs 280342 (PRKAR1A), Accession No. Hs 280378 (SNRPB2), Accession No. Hs 282410 (CALM1), Accession No. Hs 282700 (SPCS2), Accession No. Hs 282901 (RNPC2), Accession No. Hs 282998 (RBM9), Accession No. Hs 283111 (C14orf124), Accession No. Hs 283454 (BNIP2), Accession No. Hs 283521 (RHEB), Accession No. Hs 283610 (APG4B), Accession No. Hs 283652 (IDI1), Accession No. Hs 283739 (UBQLN4), Accession No. Hs 284208 (ANKRD25), Accession No. Hs 284279 (HMOX2), Accession No. Hs 284286 (MRPS24), Accession No. Hs 284491 (PDXK), Accession No. Hs 285354 (MAX), Accession No. Hs 285976 (LASS2), Accession No. Hs 286221 (ARF1), Accession No. Hs 286226 (MYO1C), Accession No. Hs 288193 (KPNA4), Accession No. Hs 288856 (PFDN5), Accession No. Hs 288969 (HSCARG), Accession No. Hs 289008 (C6orf68), Accession No. Hs 289092 (COTL1), Accession No. Hs 289123 (DCTN2), Accession No. Hs 289271 (CYC1), Accession No. Hs 290243 (GBF1), Accession No. Hs 290404 (SLC25A3), Accession No. Hs 290758 (DDB1), Accession No. Hs 291587 (ARID1B), Accession No. Hs 292026 (EIF4E2), Accession No. Hs 292063 (EIF4B), Accession No. Hs 292078 (LARP), Accession No. Hs 292265 (ZMYND11), Accession No. Hs 292457, Accession No. Hs 292493 (G22P1), Accession No. Hs 292524 (CCNH), Accession No. Hs 292579 (PTDSS1), Accession No. Hs 293563 (FLJ12666), Accession No. Hs 295917 (ATP6V1B2), Accession No. Hs 297324 (TIMP3), Accession No. Hs 298198 (CKLFSF3), Accession No. Hs 298280 (ATP5A1), Accession No. Hs 298654 (DUSP6), Accession No. Hs 299002 (FBL), Accession No. Hs 299055 (GDI2), Accession No. Hs 300141 (RPL39), Accession No. Hs 300684 (RCP9), Accession No. Hs 300772 (TPM2), Accession No. Hs 300816 RAB1B), Accession No. Hs 300834 (GALNT2), Accession No. Hs 301404 (RBM3), Accession No. Hs 301412 (Ufc1), Accession No. Hs 302742 (MRPS6), Accession No. Hs 302903 (UBE2I), Accession No. Hs 303676 (G3BP2), Accession No. Hs 304 192 (DSTN), Accession No. Hs 304682 (CST3), Accession No. Hs 306123 (MAGEF1), Accession No. Hs 306242 (RANBP9), Accession No. Hs 306329 (ZA20D3), Accession No. Hs 306425 (IBTK), Accession No. Hs 308122 (ITPK1), Accession No. Hs 308340 (NUP188), Accession No. Hs 308709 (GRP58), Accession No. Hs 309090 (SFRS7), Accession No. Hs 309231 (C6orf153), Accession No. Hs 309641 (RNF11), Accession No. Hs 309753 (STARD3NL), Accession No. Hs 309849 (C14orf159), Accession No. Hs 310542 (TOMM40), Accession No. Hs 310645 (RAB1A), Accession No. Hs 311072 (MRPS35), Accession No. Hs 311346 (CMAS), Accession No. Hs 311609 (DDX39), Accession No. Hs 311640 (RPS27A), Accession No. Hs 312098 (ADAM15), Accession No. Hs 313847 (TXNDC11), Accession No. Hs 314263 (BAZ2A), Accession No. Hs 314359 (EIF3S12), Accession No. Hs 315177 (IFRD2), Accession No. Hs 315230 (GC20), Accession No. Hs 319334 (NASP), Accession No. Hs 321391 (MGC4549), Accession No. Hs 321541 (RAB11A), Accession No. Hs 323363 (APG9L1), Accession No. Hs 323489 (FLJ20758), Accession No. Hs 324250 (NDUFB2), Accession No. Hs 324844 (VKORC1), Accession No. Hs 325650 (EHD2), Accession No. Hs 326387 (MORF4L2), Accession No. Hs 330384 (CORO1C), Accession No. Hs 331431 (SCC-112), Accession No. Hs 333388 (EEF1D), Accession No. Hs 333579 (HSPC152), Accession No. Hs 333786 (PSMA2), Accession No. Hs 333823 (MRPL13), Accession No. Hs 334017 (K-ALPHA-1), Accession No. Hs 334479 (TRAF7), Accession No. Hs 334534 (GNS), Accession No. Hs 334587 (RBPMS), Accession No. Hs 334713 (BMSC-UbP), Accession No. Hs 334851 (LASP1), Accession No. Hs 334868 (PPP2R5E), Accession No. Hs 335003 (ANKRD11), Accession No. Hs 335057 (SEPT2), Accession No. Hs 335163 (KIAA1102), Accession No. Hs 335918 (FDPS), Accession No. Hs 337295 (STIP1), Accession No. Hs 337766 (TXNRD1), Accession No. Hs 339278 (COPB), Accession No. Hs 339639 (COX7A2L), Accession No. Hs 339697 (GRINA), Accession No. Hs 343911 (EI24), Accession No. Hs 345694 (KCMF1), Accession No. Hs 346868 (EBNA1BP2), Accession No. Hs 348418 (DR1), Accession No. Hs 349656 (SCARB2), Accession No. Hs 350194 (ZMAT2), Accession No. Hs 350229 (CASC3), Accession No. Hs 350268 (IRF2BP2), Accession No. Hs 350364 (C9orf10OS), Accession No. Hs 350927 (SLC25A6), Accession No. Hs 351099 (FLJ10241), Accession No. Hs 351296 (LOC51035), Accession No. Hs 351316 (TM4SF1), Accession No. Hs 351474 (PAQR4), Accession No. Hs 351680, Accession No. Hs 351875 (COX6C), Accession No. Hs 352341 (STCH), Accession No. Hs 352656 (GHITM), Accession No. Hs 352768 (PSMB1), Accession No. Hs 354056 (POR), Accession No. Hs 355141 (TNIP1), Accession No. Hs 355606 (MGC23909), Accession No. Hs 355643 (RNPS1), Accession No. Hs 355708 (FLJ20507), Accession No. Hs 355750 (MGC5306), Accession No. Hs 355753 (DKFZp586M1819), Accession No. Hs 355867 (MARS), Accession No. Hs 355927 (VDAC2), Accession No. Hs 355934 (SFPQ), Accession No. Hs 355983 (BZW1), Accession No. Hs 356061 (MAP1LC3B), Accession No. Hs 356096 (FLJ10350), Accession No. Hs 356190 (UBB), Accession No. Hs 356270 (SDHD), Accession No. Hs 356285 (HMGN1), Accession No. Hs 356331 (PPIA), Accession No. Hs 356366 (RPS2), Accession No. Hs 356371 (RPL28), Accession No. Hs 356377 (LOC149603), Accession No. Hs 356467 (MGC2747), Accession No. Hs 356501 (PHF6), Accession No. Hs 356502 (RPLP1), Accession No. Hs 356549 (SNRPD3), Accession No. Hs 356630 (NUTF2), Accession No. Hs 356647 (SNX6), Accession No. Hs 356654 (PSMC1), Accession No. Hs 56766 (), Accession No. Hs 356769 (MAN2B1), Accession No. Hs 356799, Accession No. Hs 357901 (SOX4), Accession No. Hs 362728 (SEP15), Accession No. Hs 365116 (U2AF1), Accession No. Hs 368084 (LRPPRC), Accession No. Hs 368149 (CCT7), Accession No. Hs 368157 (PYGB), Accession No. Hs 368240 (DYRK1A), Accession No. Hs 68264 (PPP2R5C), Accession No. Hs 368376 (SRPR), Accession No. Hs 368402 (LOC51337), Accession No. Hs 368404 (EXT2), Accession No. Hs 368525 (PDLIM1), Accession No. Hs 368598 (LEREPO4), Accession No. Hs 368934 (MGC40157), Accession No. Hs 368985 (TRIP12), Accession No. Hs 369017 (RAB2), Accession No. Hs 369052 (SELT), Accession No. Hs 369068 (DNCLI2), Accession No. Hs 369125 (PSMD14), Accession No. Hs 369285 (DKFZP434B168), Accession No. Hs 369356 (MLL5), Accession No. Hs 369606 (CPSF6), Accession No. Hs 369607 (GAK), Accession No. Hs 369614 (COPS2), Accession No. Hs 369615 (FLJ20551), Accession No. Hs 369761 (DAZAP2), Accession No. Hs 369785 (MGC2749), Accession No. Hs 369920 (RAP1B), Accession No. Hs 370024 (SEC31L1), Accession No. Hs 370247 (APLP2), Accession No. Hs 370292 (BCCIP), Accession No. Hs 370312 (FNTA), Accession No. Hs 370408 (COMT), Accession No. Hs 370581 (CAP1), Accession No. Hs 370770 (XPO1), Accession No. Hs 370771 (CDKN1A), Accession No. Hs 370895 (RPN2), Accession No. Hs 370927 (PRO1855), Accession No. Hs 370937 (TAPBP), Accession No. Hs 371001 (EIF3S9), Accession No. Hs 371416 (CARM1), Accession No. Hs 371563 (RAB14), Accession No. Hs 371788 (DKFZP547E1010), Accession No. Hs 371889 (ATP1A1), Accession No. Hs 372003 (C9orf10), Accession No. Hs 372050 (SMAP-5), Accession No. Hs 372286 (CUL3), Accession No. Hs 372331 (SPTAN1), Accession No. Hs 372541 (KBTBD2), Accession No. Hs 372616 (ARL1), Accession No. Hs 372914 (NDRG1), Accession No. Hs 373550 (TGIF), Accession No. Hs 373741 (HM13), Accession No. Hs 373763 (HNRPR), Accession No. Hs 373952 (CAMTA2), Accession No. Hs 373959 (VGLL4), Accession No. Hs 374043 (ASXL1), Accession No. Hs 374257 (SIAT4A), Accession No. Hs 374378 (CKS1B), Accession No. Hs 374477 (EWSR1), Accession No. Hs 374503 (MORF4L1), Accession No. Hs 374588 (RPL17), Accession No. Hs 374596 (TPT1), Accession No. Hs 374650 (IFITM3), Accession No. Hs 374973 (PRPF4), Accession No. Hs 375001 (TLN1), Accession No. Hs 375108 (CD24), Accession No. Hs 375217 (RNF31), Accession No. Hs 376046 (BTN3A2), Accession No. Hs 376933 (GUK1), Accession No. Hs 377155 (LYRIC), Accession No. Hs 378103 (RPS5), Accession No. Hs 378532 (HBS1L), Accession No. Hs 378808 (eIF2A), Accession No. Hs 380403 (PCGF4), Accession No. Hs 380774 (DDX3X), Accession No. Hs 380953 (RPL38), Accession No. Hs 380973 (SUMO2), Accession No. Hs 381008 (HLA-E), Accession No. Hs 381058 (KIAA0146), Accession No. Hs 381072 (PPIF), Accession No. Hs 381123 (RPL21), Accession No. Hs 381126 (RPS14), Accession No. Hs 381189 (CBX3), Accession No. Hs 381219, Accession No. Hs 381256 (GLTP), Accession No. Hs 382044 (MRPS2), Accession No. Hs 382168 (NCOA3), Accession No. Hs 385913 (ANP32E), Accession No. Hs 385986 (UBE2B), Accession No. Hs 386434 (ANXA7), Accession No. Hs 386465 (CHERP), Accession No. Hs 386939 (USP7), Accession No. Hs 387208 (FAU), Accession No. Hs 387804 (PABPC1), Accession No. Hs 388034 (RXRB), Accession No. Hs 388654 (ATP6V1G1), Accession No. Hs 388664 (RPL11), Accession No. Hs 388739 (XRCC5), Accession No. Hs 388927 (YY1), Accession No. Hs 388956 (C19orf22), Accession No. Hs 389037 (MCM3APAS), Accession No. Hs 389107 (ATP6V0C), Accession No. Hs 389171 (PINK1), Accession No. Hs 389649 (DDX48), Accession No. Hs 389734 (TCEAL8), Accession No. Hs 389996 (CHCHD2), Accession No. Hs 390667 (GSTK1), Accession No. Hs 393201 (ACTR2), Accession No. Hs 395482 (PTK2), Accession No. Hs 396644 (PAIP2), Accession No. Hs 396740 (NIP30), Accession No. Hs 396783 (SLC9A3R1), Accession No. Hs 397609 (RPS16), Accession No. Hs 399800 (AKAP8L), Accession No. Hs 400295 (RPL30), Accession No. Hs 401509 (RBM10), Accession No. Hs 401903 (COX5A), Accession No. Hs 401929 (RPL10), Accession No. Hs 403917 (STK24), Accession No. Hs 404056 (EIF3S1), Accession No. Hs 404321 (GARS), Accession No. Hs 405144 (SFRS3), Accession No. Hs 405410 (OGT), Accession No. Hs 405514 (LOC284058), Accession No. Hs 405590 (EIF3S6), Accession No. Hs 405880 (MRPS21), Accession No. Hs 405942 (LOC339229), Accession No. Hs 406062 (NDUFA11), Accession No. Hs 406068 (UBE2M), Accession No. Hs 406096 (ZA20D2), Accession No. Hs 406277 (SF3A1), Accession No. Hs 406300 (RPL23), Accession No. Hs 406423 (SF3B2), Accession No. Hs 406510 (ATP5B), Accession No. Hs 406520 (LOC389541), Accession No. Hs 406534 (HMG20B), Accession No. Hs 406590 (PGR1), Accession No. Hs 406620 (RPS10), Accession No. Hs 406683 (RPS15), Accession No. Hs 406799 (RAB18), Accession No. Hs 406840 (SLC35A4), Accession No. Hs 407368 (C19orf13), Accession No. Hs 407580 (PKP4), Accession No. Hs 407995 (MIF), Accession No. Hs 408018 (RPL36), Accession No. Hs 408073 (RPS6), Accession No. Hs 408236 (TXNL5), Accession No. Hs 408257 (NDUFS6), Accession No. Hs 408293 (KAB), Accession No. Hs 408324 (FLJ10769), Accession No. Hs 408428 (CHES1), Accession No. Hs 408581 (SVIL), Accession No. Hs 408909 (GOLPH3), Accession No. Hs 409140 (ATP5O), Accession No. Hs 409223 (SSR4), Accession No. Hs 409230 (AGPAT1), Accession No. Hs 409834 (PHPT1), Accession No. Hs 410197 (IDH3G), Accession No. Hs 410596 (HAN11), Accession No. Hs 410817 (RPL13), Accession No. Hs 411480 (AUP1), Accession No. Hs 411641 (EIF4EBP1), Accession No. Hs 411847 (MAPK6), Accession No. Hs 412103 (EFHA1), Accession No. Hs 412117 (ANXA6), Accession No. Hs 412196 (ESRRBL1), Accession No. Hs 412433 (AIP), Accession No. Hs 412468 (KLHDC3), Accession No. Hs 412842 (C10orf7), Accession No. Hs 413036 (WBSCR22), Accession No. Hs 413482 (C21orf33), Accession No. Hs 414579 (SCOTIN), Accession No. Hs 415342 (KIAA1049), Accession No. Hs 416049 (TNPO2), Accession No. Hs 416436 (TRIM50A), Accession No. Hs 417004 (S100A11), Accession No. Hs 417029 (DERP6), Accession No. Hs 418123 (CTSL), Accession No. Hs 418175 (VPS28), Accession No. Hs 418233 (MRPL24), Accession No. Hs 418450 (MRPL11), Accession No. Hs 418533 (BUB3), Accession No. Hs 418668 (ATP5D), Accession No. Hs 419640 (PARK7), Accession No. Hs 420269 (COL6A2), Accession No. Hs 420272 (H2AFY), Accession No. Hs 421257 (RPL7), Accession No. Hs 421509 (CCT4), Accession No. Hs 422113 (ZNF511), Accession No. Hs 423935 (RDBP), Accession No. Hs 423968 (TTC11), Accession No. Hs 424126 (SERF2), Accession No. Hs 424908 (LSM5), Accession No. Hs 425777 (UBE2L6), Accession No. Hs 426296 (C10orf104), Accession No. Hs 426359 (DKFZp564J157), Accession No. Hs 429052 (ITGB1), Accession No. Hs 429353 (SEPN1), Accession No. Hs 429581 (RTN4), Accession No. Hs 429819 (PITPNA), Accession No. Hs 429839 (MGC23908), Accession No. Hs 430425 (GNB1), Accession No. Hs 430551 (IQGAP1), Accession No. Hs 430606 (CS), Accession No. Hs 430657 (ARF5), Accession No. Hs 430733 (CLNS1A), Accession No. Hs 431101 (GNG12), Accession No. Hs 431367 (C6orf55), Accession No. Hs 431498 (FOXP1), Accession No. Hs 431550 (MAP4K4), Accession No. Hs 431668 (COX6B1), Accession No. Hs 431850 (MAPK1), Accession No. Hs 431861 (PPP5C), Accession No. Hs 431926 NFKB1), Accession No. Hs 432121 (PRDX2), Accession No. Hs 432438 (EML4), Accession No. Hs 432491 (ESD), Accession No. Hs 432690 (SLC39A9), Accession No. Hs 432760 (CAPZB), Accession No. Hs 432898 (RPL4), Accession No. Hs 432976 (NR1H2), Accession No. Hs 433154 (PLSCR3), Accession No. Hs 433201 (CDK2AP1), Accession No. Hs 433222 (NPC2), Accession No. Hs 433291 (ARD1), Accession No. Hs 433307 (BCKDHA), Accession No. Hs 433343 (SRRM2), Accession No. Hs 433345, Accession No. Hs 433419 (COX4I1), Accession No. Hs 433512 (ACTR3), Accession No. Hs 433529 (RPS11), Accession No. Hs 433540 (DNAJC8), Accession No. Hs 433573 (Bles03), Accession No. Hs 433615 (TUBB2), Accession No. Hs 433701 (RPL37A), Accession No. Hs 433722 (KIAA1967), Accession No. Hs 33732 (CLK1), Accession No. Hs 433750 (EIF4G1), Accession No. Hs 433759 (BANF1), Accession No. Hs 433795 (SHC1), Accession No. Hs 433863 (PBP), Accession No. Hs 433901 (COX8A), Accession No. Hs 433951 (GPX4), Accession No. Hs 434102 (HMGB1), Accession No. Hs 434207 (HARS2), Accession No. Hs 434219 (ANKHD1), Accession No. Hs 434401 (ZNF638), Accession No. Hs 434937 (PPIB), Accession No. Hs 434953 (HMGB2), Accession No. Hs 434980 (APP), Accession No. Hs 435044 (TBC1D22A), Accession No. Hs 435064 (KIAA1608), Accession No. Hs 435120 (KIF1C), Accession No. Hs 435136 (TXN), Accession No. Hs 435166 (LBR), Accession No. Hs 435231 (ZFR), Accession No. Hs 435255 (UBXD1), Accession No. Hs 435326 (ACTL6A), Accession No. Hs 435512 (PPP3CA), Accession No. Hs 435535 (ZNF395), Accession No. Hs 435610 (WAC), Accession No. Hs 435741 (GCSH), Accession No. Hs 435759 (THAP4), Accession No. Hs 435771 (API5), Accession No. Hs 435841 (TNRC15), Accession No. Hs 435850 (LYPLA1), Accession No. Hs 435933 (PHF10), Accession No. Hs 435948 (ATAD1), Accession No. Hs 435952 (CDK5RAP1), Accession No. Hs 435974 (MTHFD1), Accession No. Hs 436035 (TUBA6), Accession No. Hs 436093 (BAT2), Accession No. Hs 436204 (ZNF289), Accession No. Hs 436298 (EMP1), Accession No. Hs 436405 (IDH3B), Accession No. Hs 436437 (ALDH2), Accession No. Hs 436446 (ARMET), Accession No. Hs 436500 (DBNL), Accession No. Hs 436568 (CD74), Accession No. Hs 436578 (POLR2F), Accession No. Hs 436657 (CLU), Accession No. Hs 436687 (SET), Accession No. Hs 436803 (VBP1), Accession No. Hs 437056 (SUPT5H), Accession No. Hs 437060 (CYCS), Accession No. Hs 437110 (ANXA2), Accession No. Hs 437178 (ACADVL), Accession No. Hs 437256 (GRINL1A), Accession No. Hs 437277 (MGAT4B), Accession No. Hs 437367 (GBAS), Accession No. Hs 437388 (PIGT), Accession No. Hs 437403 (PP), Accession No. Hs 437594 (RPLP2), Accession No. Hs 437638 (XBP1), Accession No. Hs 437779 (C11orf10), Accession No. Hs 437831 (C14orf32), Accession No. Hs 438072 (UNC84A), Accession No. Hs 438219 (GPS2), Accession No. Hs 438429 (RPS19), Accession No. Hs 438678 (TALDO1), Accession No. Hs 438720 (MCM7), Accession No. Hs 438970 (TBL1XR1), Accession No. Hs 438974 (CUTL1), Accession No. Hs 439480 (RBM5), Accession No. Hs 439481 (SUPT4H1), Accession No. Hs 439548 (FLJ22875), Accession No. Hs 439552, Accession No. Hs 439815 (HBXIP), Accession No. Hs 440382 (RFP), Accession No. Hs 440544 (CLIC4), Accession No. Hs 440599 (DDX1), Accession No. Hs 440604 (PSMD7), Accession No. Hs 440899 (TTYH3), Accession No. Hs 440932 (SEPT9), Accession No. Hs 440960 (RAD23A), Accession No. Hs 440961 (CAST), Accession No. Hs 441072 (POLR2L), Accession No. Hs 441550 (C20orf22), Accession No. Hs 442344 (IRS2), Accession No. Hs 442798 (RNF10), Accession No. Hs 443134 (GBA2), Accession No. Hs 443379 (PSMD11), Accession No. Hs 443837 (NPEPPS), Accession No. Hs 443914 (SOD1), Accession No. Hs 444279 (DKFZp761C169), Accession No. Hs 444356 (GRB2), Accession No. Hs 444468 (CTDSP1), Accession No. Hs 444472 (SDHC), Accession No. Hs 444569 (VMP1), Accession No. Hs 444673 (CRR9), Accession No. Hs 444724 (AZI2), Accession No. Hs 444818 (CGGBP1), Accession No. Hs 444931 (CRSP6), Accession No. Hs 444969 (C2orf4), Accession No. Hs 444986 (METAP2), Accession No. Hs 445081 (NS5ATP13TP2), Accession No. Hs 445351 (LGALS1), Accession No. Hs 445394 (VPS29), Accession No. Hs 445498 (SKIIP), Accession No. Hs 445511 (RIOK3), Accession No. Hs 445570 (CD63), Accession No. Hs 445803 (DC2), Accession No. Hs 445893 (KHDRBS1), Accession No. Hs 445977 (GTF3A), Accession No. Hs 446017 (WSB1), Accession No. Hs 446091 (WTAP), Accession No. Hs 446123 (CAPZA2), Accession No. Hs 446149 (LDHB), Accession No. Hs 446260 (PSMA6), Accession No. Hs 446336 (PXN), Accession No. Hs 446345 (FTH1), Accession No. Hs 446414 (CD47), Accession No. Hs 446427 (OAZ1), Accession No. Hs 446445 (YIF1), Accession No. Hs 446450 (ITM2B), Accession No. Hs 446574 (TMSB10), Accession No. Hs 446588 (RPS13), Accession No. Hs 446623 (HNRPL), Accession No. Hs 446628 (RPS4X), Accession No. Hs 446641 (ARAF), Accession No. Hs 446852 (EIF3S6IP), Accession No. Hs 447477 (ST13), Accession No. Hs 447492 (PGAM1), Accession No. Hs 447547 (VPS35), Accession No. Hs 48226 (RPLP0), Accession No. Hs 448588 (NGFRAP1), Accession No. Hs 448646 (RPL27A), Accession No. Hs 448879, Accession No. Hs 449114 (HNRPC), Accession No. Hs 449171 (HNRPK), Accession No. Hs 454534 (USF2), Accession No. Hs 454699 (IL6ST), Accession No. Hs 456507 (PKD1-like), Accession No. Hs 456557 (FLJ10597), Accession No. Hs 458320 (DC12), Accession No. Hs 458358 (TSPYL1), Accession No. Hs 458414 (IFITM1), Accession No. Hs 458458 (C19orf27), Accession No. Hs 458747 (ANP32A), Accession No. Hs 459106 (OAZIN), Accession No. Hs 459149 (BTBD1), Accession No. Hs 459174 (FLJ23790), Accession No. Hs 459211 (AKAP13), Accession No. Hs 459596 (MPG), Accession No. Hs 459649 (CLCN7), Accession No. Hs 459927 (PTMA), Accession No. Hs 459940 (LITAF), Accession No. Hs 460238 (SH3GLB2), Accession No. Hs 460317 (ALS4), Accession No. Hs 460336 (GGA2), Accession No. Hs 460468 (XPO6), Accession No. Hs 460499 (ATXN2L), Accession No. Hs 460574 (LOC124446), Accession No. Hs 460923 (CNOT1), Accession No. Hs 460929 (GOT2), Accession No. Hs 460978 (APPBP1), Accession No. Hs 461047 (G6PD), Accession No. Hs 461131 (CYB5-M), Accession No. Hs 461361 (CFDP1), Accession No. Hs 461379 (GABARAPL2), Accession No. Hs 461722 (HSPC176), Accession No. Hs 461777 (PCOLN3), Accession No. Hs 461896 (CRK), Accession No. Hs 461925 (RPA1), Accession No. Hs 462035 (UBE2G1), Accession No. Hs 462086 (RIP), Accession No. Hs 462306 (UBE2S), Accession No. Hs 462316 (TTC19), Accession No. Hs 462492 (USP22), Accession No. Hs 462550 (PIGS), Accession No. Hs 462956 (PPARBP), Accession No. Hs 462998 (IGFBP4), Accession No. Hs 463010 (SMARCE1), Accession No. Hs 463035 (FKBP10), Accession No. Hs 463041 (RERE), Accession No. Hs 463059 (STAT3), Accession No. Hs 463295 (CDC27), Accession No. Hs 463506 (AKAP1), Accession No. Hs 463702 (BCAS3), Accession No. Hs 463797 (C1orf33), Accession No. Hs 464071 (PGD), Accession No. Hs 464137 (ACOX1), Accession No. Hs 464210 (SYNGR2), Accession No. Hs 464336 (P4HB), Accession No. Hs 464438 (AGTRAP), Accession No. Hs 464472 (MRLC2), Accession No. Hs 464595 (PPP4R1), Accession No. Hs 464652 (TNFSF5IP1), Accession No. Hs 464912 (P15RS), Accession No. Hs 465224 (NARS), Accession No. Hs 465374 (EFHD2), Accession No. Hs 465498 (TXNL4A), Accession No. Hs 465529 (MIDN), Accession No. Hs 465543 (BTBD2), Accession No. Hs 465627 (MAP2K2), Accession No. Hs 465645 (C19orf10), Accession No. Hs 465808 (HNRPM), Accession No. Hs 465849 (PIN1), Accession No. Hs 465924 (SDHB), Accession No. Hs 466044 (PKN1), Accession No. Hs 466088 (TPM4), Accession No. Hs 466148 (NR2F6), Accession No. Hs 466471 (GPI), Accession No. Hs 466693 (SIRT2), Accession No. Hs 466766 (LTBP4), Accession No. Hs 466775 (SNRPA), Accession No. Hs 467084 (EIF4G3), Accession No. Hs 467097 (SNRP70), Accession No. Hs 467192 (PPP2R1A), Accession No. Hs 467279 (LENG4), Accession No. Hs 467284 (RPS9), Accession No. Hs 467408 (TRIM28), Accession No. Hs 467637 (CDC42), Accession No. Hs 467696 (HPCAL1), Accession No. Hs 467701 (ODC1), Accession No. Hs 467807 (LAPTM4A), Accession No. Hs 467824 (PUM2), Accession No. Hs 467960 (RAB10), Accession No. Hs 468018 (PPP1CB), Accession No. Hs 468415 (PIGF), Accession No. Hs 468442 (CALM2), Accession No. Hs 468760 (AFTIPHILIN), Accession No. Hs 469022 (DGUOK), Accession No. Hs 469171 (DKFZP564D0478), Accession No. Hs 469331 (STARD7), Accession No. Hs 469820 (RALB), Accession No. Hs 469863 (YWHAZ), Accession No. Hs 469925 (FLJ14346), Accession No. Hs 469970 (SFRS4), Accession No. Hs 470091 (YWHAE), Accession No. Hs 470233 (ARL5), Accession No. Hs 470417, Accession No. Hs 470477 (PTP4A2), Accession No. Hs 470577 (EIF2S2), Accession No. Hs 470588 (KPNA6), Accession No. Hs 470943 (STAT1), Accession No. Hs 471011 (SF3B1), Accession No. Hs 471104 (NOP5 / NOP58), Accession No. Hs 471207 (NDUFS1), Accession No. s 471441 (PSMB2), Accession No. Hs 471461 (ACSL3), Accession No. Hs 471593 (CAB39), Accession No. Hs 471768 (MGC4796), Accession No. Hs 471818 (M11S1), Accession No. Hs 471851 (HDLBP), Accession No. Hs 471873 (DTYMK), Accession No. Hs 471933 (FKBP1A), Accession No. Hs 471975 (C20orf116), Accession No. Hs 472010 (PRNP), Accession No. Hs 472024 (C20orf30), Accession No. Hs 472031 (UBE2D3), Accession No. Hs 472038 (CGI-94), Accession No. Hs 472056 (SYNCRIP), Accession No. Hs 472119 (MKKS), Accession No. Hs 472185 (NDUFS5), Accession No. Hs 472213 (RRBP1), Accession No. Hs 472330 (C20orf3), Accession No. Hs 472475 (MACF1), Accession No. Hs 472535 (AKIP), Accession No. Hs 472558 (SDBCAG84), Accession No. Hs 472651 (BLCAP), Accession No. Hs 472737 (TOP1), Accession No. Hs 473296 (TPD52L2), Accession No. Hs 473583 (NSEP1), Accession No. Hs 473648 (GART), Accession No. Hs 473721 (SLC2A1), Accession No. Hs 473761 (RTN3), Accession No. Hs 473788 (OTUB1), Accession No. Hs 474005 (SUMO3), Accession No. Hs 474010 (PTTG1IP), Accession No. Hs 474053 (COL6A1), Accession No. Hs 474083 (B4GALT2), Accession No. Hs 474213 (UFD1L), Accession No. Hs 474584 (AKR1A1), Accession No. Hs 474643 (HSPC117), Accession No. Hs 474751 (MYH9), Accession No. Hs 474833 (CSNK1E), Accession No. Hs 474914 (RUTBC3), Accession No. Hs 474938 (SLC25A17), Accession No. Hs 474949 (RBX1), Accession No. Hs 474982 (ACO2), Accession No. Hs 475125 (ATXN10), Accession No. Hs 475319 (LRRFIP2), Accession No. Hs 475382 (FLJ22405), Accession No. Hs 475392 (LOC55831), Accession No. Hs 475663 (RAB5A), Accession No. Hs 475733 (TOP2B), Accession No. Hs 475812 (SIMP), Accession No. Hs 476018 (CTNNB1), Accession No. Hs 476033 (TLP19), Accession No. Hs 476179 (SMARCC1), Accession No. Hs 476221 (IHPK2), Accession No. Hs 76231 (IMPDH2), Accession No. Hs 476308 (ALAS1), Accession No. Hs 476365 (SCP2), Accession No. Hs 476448 (FLNB), Accession No. Hs 476706 (MRPL37), Accession No. Hs 476930 (DKFZP564O123), Accession No. Hs 477157 (DULLARD), Accession No. Hs 477789 (ATP1B3), Accession No. Hs 477892 (GYG), Accession No. Hs 478000 (MBNL1), Accession No. Hs 478044 (PA2G4), Accession No. Hs 478553 (EIF4A2), Accession No. Hs 479208 (FBXL5), Accession No. Hs 479264 (LAP3), Accession No. Hs 479634 (SLC30A9), Accession No. Hs 479693 (SFRS11), Accession No. Hs 479728 (GAPD), Accession No. Hs 479747 (BCAR1), Accession No. Hs 479814 (POLR2B), Accession No. Hs 480073 (HNRPD), Accession No. Hs 480311 (PDLIM5), Accession No. Hs 480465 (SCYE1), Accession No. Hs 80653 (ANXA5), Accession No. Hs 481571 (UQCRH), Accession No. Hs 481720 (MYO10), Accession No. Hs 481898 (KAT3), Accession No. Hs 482144 (RPL26), Accession No. Hs 482363 (SLC30A5), Accession No. Hs 482526 (TINP1), Accession No. Hs 482868 (KIAA0372), Accession No. Hs 483036 (PJA2), Accession No. Hs 483067 (C5orf13), Accession No. Hs 483305 (HINT1), Accession No. Hs 483408 (PPP2CA), Accession No. Hs 483454 (CNN3), Accession No. Hs 483486 (JMJD1B), Accession No. Hs 484138 (FBXW11), Accession No. Hs 484188 (ATP6V0E), Accession No. 484242 (ETEA), Accession No. Hs 484288 (DDX41), Accession No. Hs 484363 (RNF130), Accession No. Hs 484551 (CPM), Accession No. Hs 484813 (DEK), Accession No. Hs 485155 (RPL35), Accession No. Hs 485195 (SORT1), Accession No. Hs 485246 (PSMA5), Accession No. Hs 485262 (MTCH1), Accession No. Hs 485365 (AHCYL1), Accession No. Hs 485616 (DST), Accession No. Hs 486542 (BCLAF1), Accession No. Hs 487027 (VIL2), Accession No. Hs 487054 (TCP1), Accession No. Hs 487635 (BZW2), Accession No. Hs 487774 (HNRPA2B1), Accession No. Hs 488171 (KIAA1068), Accession No. Hs 488181 (OGDH), Accession No. Hs 488307 (DKFZP564K0822), Accession No. Hs 488478 (FLJ10099), Accession No. Hs 488671 (BAZ1B), Accession No. Hs 489207 (ASNS), Accession No. Hs 489284 (ARPC1B), Accession No. Hs 489287 (CPSF4), Accession No. Hs 489336 (SYAP1), Accession No. Hs 489615 (PBEF1), Accession No. Hs 490203 (CALD1), Accession No. Hs 490394 (SSBP1), Accession No. Hs 490415 (ZYX), Accession No. Hs 490745 (DNAJB6), Accession No. Hs 490795 (CHR2SYT), Accession No. Hs 490874 (MTX1), Accession No. Hs 491336 (ELP3), Accession No. Hs 491359 (LMNA), Accession No. Hs 491440 (PPP2CB), Accession No. Hs 491494 (CCT3), Accession No. Hs 491597 (VDAC3), Accession No. Hs 491695 (UBE2V2), Accession No. Hs 491745 (TCEA1), Accession No. Hs 491988 (TRAM1), Accession No. Hs 492236 (WDR42A), Accession No. Hs 492314 (LAPTM4B), Accession No. Hs 492445 (EDD), Accession No. Hs 492599 (EIF3S3), Accession No. Hs 492805 (CGI-07), Accession No. Hs 493362 (AK3L1), Accession No. Hs 493750 (WDR40A), Accession No. Hs 494173 (ANXA1), Accession No. Hs 494419 (LAMP1), Accession No. Hs 494457 (NINJ1), Accession No. Hs 494604 (ANP32B), Accession No. Hs 494614 (XTP2), Accession No. Hs 494691 (PFN1), Accession No. Hs 494700 (CDW92), Accession No. Hs 494985 (FBXW2), Accession No. Hs 495039 (NDUFA8), Accession No. Hs 495349 (KIAA0515), Accession No. Hs 495471 (PMPCA), Accession No. Hs 495605 (CD99), Accession No. Hs 495851 (MGC4825), Accession No. Hs 495960 (ATP6AP2), Accession No. Hs 496068 (PCTK1), Accession No. Hs 496098 (DKFZp761A052), Accession No. Hs 496271, Accession No. Hs 496487 (ATF4), Accession No. Hs 496646 (IL13RA1), Accession No. Hs 496684 (LAMP2), Accession No. Hs 497183 (IVNS1ABP), Accession No. Hs 497599 (WARS), Accession No. Hs 497692 (C1orf48), Accession No. Hs 497893 (ENAH), Accession No. Hs 498239 (FH), Accession No. Hs 498313 (ADSS), Accession No. Hs 498317 (PNAS-4), Accession No. Hs 498455 (KIAA0217), Accession No. Hs 498548 (RBM17), Accession No. Hs 498727 (DHCR24), Accession No. Hs 499145 (YME1L1), Accession No. Hs 499158 (GGA1), Accession No. Hs 499594 (TIMM23), Accession No. Hs 499833 (C10orf74), Accession No. Hs 499891 (HNRPH3), Accession No. Hs 499925 (VPS26), Accession No. Hs 499960 (SARA1), Accession No. Hs 500067 (PPP3CB), Accession No. Hs 500101 (VCL), Accession No. Hs 500375 (ENTPD6), Accession No. Hs 500409 (GLUD1), Accession No. Hs 500546 (IDE), Accession No. Hs 500674 (SMBP), Accession No. Hs 500775 (ZNF207), Accession No. Hs 500842 (MGEA5), Accession No. Hs 500874 (CUEDC2), Accession No. Hs 501012 (ADD3), Accession No. Hs 501023 (MXI1), Accession No. Hs 501203 (TIAL1), Accession No. Hs 501293 (BSG), Accession No. Hs 501309 (CIRBP), Accession No. Hs 501353 (PLEKHJ1), Accession No. Hs 501376 (UROS), Accession No. Hs 501420 (NCLN), Accession No. Hs 501629 (IER2), Accession No. Hs 501684 (NAP1L4), Accession No. Hs 501735 (STIM1), Accession No. Hs 501853 (C11orf15), Accession No. Hs 501924 (USP47), Accession No. Hs 501991 (MLSTD2), Accession No. Hs 502302 (CAT), Accession No. Hs 502328 (CD44), Accession No. Hs 502461 (DGKZ), Accession No. Hs 502528 (NDUFS3), Accession No. Hs 502630 (C11orf31), Accession No. Hs 502659 (RHOC), Accession No. Hs 502705 (PRP19), Accession No. Hs 502745 (FADS2), Accession No. Hs 502769 (SLC3A2), Accession No. Hs 502773 (MTCBP-1), Accession No. Hs 502823 (PRDX5), Accession No. Hs 502829 (SF1), Accession No. Hs 502836 (ARL2), Accession No. Hs 502842 (CAPN1), Accession No. Hs 502872 (MAP3K11), Accession No. Hs 502876 (RHOB), Accession No. Hs 503093 (ZFP36L2), Accession No. Hs 503222 (RAB6A), Accession No. Hs 503251 (PME-1), Accession No. Hs 503597 (HSPC148), Accession No. Hs 503709 (PORIMIN), Accession No. Hs 503716 (MGC2714), Accession No. Hs 503787 (DARS), Accession No. Hs 504237 (ITM1), Accession No. Hs 504517 (RPS27), Accession No. Hs 504613 (PTMS), Accession No. Hs 504620 (REA), Accession No. Hs 504687 (MYL9), Accession No. Hs 504828 (DDX47), Accession No. Hs 504895 (STRAP), Accession No. Hs 505033 (KRAS2), Accession No. Hs 505059 (PSMD4), Accession No. Hs 505625 (C12orf10), Accession No. Hs 505652 (COPZ1), Accession No. Hs 505676 (CIP29), Accession No. Hs 505705 (MYL6), Accession No. Hs 505806 (PBXIP1), Accession No. Hs 505824 (CGI-51), Accession No. Hs 506215 (RARS), Accession No. Hs 506325 (NUDT4), Accession No. Hs 06759 (ATP2A2), Accession No. Hs 506861 (DDX54), Accession No. Hs 507074 (KIAA0152), Accession No. Hs 507162 (FLJ12750), Accession No. Hs 507584 (MGC9850), Accession No. Hs 507680 (PFAAP5), Accession No. Hs 507910 (PGRMC2), Accession No. Hs 507916 (TGFB1I4), Accession No. Hs 508010 (FNDC3A), Accession No. Hs 508644 (FLJ10154), Accession No. Hs 509163 (KIAA1181), Accession No. Hs 509226 (FKBP3), Accession No. Hs 509264 (KLHDC2), Accession No. Hs 509414 (KTN1), Accession No. Hs 509622 (RGL2), Accession No. Hs 509736 (HSPCB), Accession No. Hs 509791 (ERH), Accession No. Hs 509909 (NUMB), Accession No. Hs 510087 (ENSA), Accession No. Hs 510328 (DDX24), Accession No. Hs 510402 (MCP), Accession No. Hs 511067 (FLJ10579), Accession No. Hs 511138 (DKFZP564G2022), Accession No. Hs 511149 (SNAP23), Accession No. Hs 511425 (SRP9), Accession No. Hs 511504 (TCF12), Accession No. Hs 511862, Accession No. Hs 511952 (CBX6), Accession No. Hs 512005 (ARPC3), Accession No. Hs 512465 (SURF4), Accession No. Hs 512525 (RPS17), Accession No. Hs 512607 (MIR16), Accession No. Hs 512640 (PRKCSH), Accession No. Hs 512661 (KIAA1160), Accession No. Hs 512676, Accession No. Hs 512693 (FLJ20859), Accession No. Hs 512756 (THAP7), Accession No. Hs 512815 (AP3D1), Accession No. Hs 512857 (CD151), Accession No. Hs 512867 (H63), Accession No. Hs 512908 (ARPP-19), Accession No. Hs 513043 (C15orf12), Accession No. Hs 513055 (REC14), Accession No. Hs 513057 (RANBP5), Accession No. Hs 513058 (TMED3), Accession No. Hs 513071 (MESDC1), Accession No. Hs 513083 (RPL9), Accession No. Hs 513141 (IDH2), Accession No. Hs 513145 (NEUGRIN), Accession No. Hs 513153 (FURIN), Accession No. Hs 513230 (MRPL28), Accession No. Hs 513242 (RHOT2), Accession No. Hs 513261 (C16orf34), Accession No. Hs 513266 (NDUFB10), Accession No. Hs 513470 (NFATC2IP), Accession No. Hs 513488 (MVP), Accession No. Hs 513490 (ALDOA), Accession No. Hs 513520 (BCKDK), Accession No. Hs 513522 (FUS), Accession No. Hs 513631 (ARL2BP), Accession No. Hs 513856 (DPH2L1), Accession No. Hs 513984 (FLII), Accession No. Hs 514012 (MAP2K3), Accession No. Hs 514036 (SDF2), Accession No. Hs 514038 (FLOT2), Accession No. Hs 514174 (JUP), Accession No. Hs 514196 (RPL27), Accession No. Hs 514211 (MGC4251), Accession No. Hs 514216 (CGI-69), Accession No. Hs 514220 (GRN), Accession No. Hs 514297 (FLJ13855), Accession No. Hs 514303 (PHB), Accession No. Hs 514435 (SF3B3), Accession No. Hs 514489 (WBP2), Accession No. Hs 514535 (LGALS3BP), Accession No. Hs 514581 (ACTG1), Accession No. Hs 514590 (HGS), Accession No. Hs 514819 (AP2B1), Accession No. Hs 514870 (ATP5F1), Accession No. Hs 514920 (NDP52), Accession No. Hs 514934 (CAPZA1), Accession No. Hs 515003 (C19orf6), Accession No. Hs 515005 (STK11), Accession No. Hs 515018 (GNA13), Accession No. Hs 515053 (AES), Accession No. Hs 515070 (EEF2), Accession No. Hs 515092 (CLPP), Accession No. Hs 515155 (MGC2803), Accession No. Hs 515162 (CALR), Accession No. Hs 515164 (GADD45GIP1), Accession No. Hs 515210 (DNAJB1), Accession No. Hs 515255 (LSM4), Accession No. Hs 515266 (RENT1), Accession No. Hs 515271 (SFRS14), Accession No. Hs 515329 (RPL22), Accession No. Hs 515371 (CAPNS1), Accession No. Hs 515406 (AKT2), Accession No. Hs 515417 (EGLN2), Accession No. Hs 515432 (DEDD2), Accession No. Hs 515472 (SNRPD2), Accession No. Hs 515475 (SYMPK), Accession No. Hs 515487 (CALM3), Accession No. Hs 515494 (SLC1A5), Accession No. Hs 515500 (SAE1), Accession No. Hs 515515 (KDELR1), Accession No. Hs 515517 (RPL18), Accession No. Hs 515524 (NUCB1), Accession No. Hs 515540 (PTOV1), Accession No. Hs 515550 (LOC284361), Accession No. Hs 515598 (PRPF31), Accession No. Hs 515607 (PPP1R12C), Accession No. Hs 515642 (GPSN2), Accession No. Hs 515785 (BLVRB), Accession No. Hs 515846 (RUVBL2), Accession No. Hs 515848 (HADHB), Accession No. Hs 515890 (YPEL5), Accession No. Hs 516075 (TIA1), Accession No. Hs 516077 (FLJ14668), Accession No. Hs 516087 (TEX261), Accession No. Hs 516111 (DCTN1), Accession No. Hs 516114 (WBP1), Accession No. Hs 516157 (MAT2A), Accession No. Hs 516450 (FLJ20297), Accession No. Hs 516522 (FLJ21919), Accession No. Hs 516539 (HNRPA3), Accession No. Hs 516587 (UBE2Q), Accession No. Hs 516633 (NCKAP1), Accession No. Hs 516711 (CHPF), Accession No. Hs 516790 (ARHGEF2), Accession No. Hs 516807 (STK25), Accession No. Hs 516826 (TRIB3), Accession No. Hs 516855 (CENPB), Accession No. Hs 517080 (SLC35C2), Accession No. Hs 517106 (CEBPB), Accession No. Hs 517134 (C20orf43), Accession No. Hs 517145 (ENO1), Accession No. Hs 517168 (TAGLN2), Accession No. Hs 517216 (PEA15), Accession No. Hs 517232 (PEX19), Accession No. Hs 517240 (IFNGR2), Accession No. Hs 517262 (SON), Accession No. Hs 517293 (F11R), Accession No. Hs 517338 (ATP6V1E1), Accession No. Hs 17342 (DEDD), Accession No. Hs 517356 (COL18A1), Accession No. Hs 517357 (DGCR2), Accession No. Hs 517421 (PCQAP), Accession No. Hs 517438 (ASCC2), Accession No. Hs 517517 (EP300), Accession No. Hs 517543 (PES1), Accession No. Hs 517582 (MCM5), Accession No. Hs 517622 (UNC84B), Accession No. Hs 517641 (L3MBTL2), Accession No. Hs 517666 (DIA1), Accession No. Hs 517731 (PP2447), Accession No. Hs 517768 (DKFZP564B167), Accession No. Hs 517792 (C3orf10), Accession No. Hs 517817 (MGC3222), Accession No. Hs 517821, Accession No. Hs 517888 (CRTAP), Accession No. Hs 517948 (DHX30), Accession No. Hs 517949 (MAP4), Accession No. Hs 517969 (APEH), Accession No. Hs 517981 (TUSC2), Accession No. Hs 518060 (ARL6IP5), Accession No. Hs 518123 (TFG), Accession No. Hs 518236 (SEC61A1), Accession No. Hs 518244 (RPN1), Accession No. Hs 518249 (ZNF9), Accession No. Hs 518250 (COPG), Accession No. Hs 518265 (H41), Accession No. Hs 518326 (SERP1), Accession No. Hs 518346 (SSR3), Accession No. Hs 518374 (QSCN6), Accession No. Hs 518424 (NDUFB5), Accession No. Hs 518460 (AP2M1), Accession No. Hs 518464 (PSMD2), Accession No. Hs 518525 (GLUL), Accession No. Hs 518551 (RPL31), Accession No. Hs 518608 (PP784), Accession No. Hs 518609 (ARPC5), Accession No. Hs 518750 (OCIAD1), Accession No. Hs 18805 (HMGA1), Accession No. Hs 518827 (CCNI), Accession No. Hs 519276 (MAPKAPK2), Accession No. Hs 519304 (PELO), Accession No. Hs 519346 (ERBB2IP), Accession No. Hs 519347 (SFRS12), Accession No. Hs 519520 (RPS25), Accession No. Hs 519523 (SERPINB6), Accession No. Hs 519557 (TMEM14C), Accession No. Hs 519718 (TTC1), Accession No. Hs 519756 (STK10), Accession No. Hs 519818 (MGAT1), Accession No. Hs 519909 (MARCKS), Accession No. Hs 519930 (C6orf62), Accession No. Hs 520026 (VARS2), Accession No. Hs 520028 (HSPA1A), Accession No. Hs 520037 (NEU1), Accession No. Hs 520070 (C6orf82), Accession No. Hs 520140 (SRF), Accession No. Hs 520189 (ELOVL5), Accession No. Hs 520205 (EIF2AK1), Accession No. Hs 520210 (KDELR2), Accession No. Hs 520287 (C6orf111), Accession No. Hs 520313 (CD164), Accession No. Hs 520383 (STX7), Accession No. Hs 520421 (PERP), Accession No. Hs 520459 (GTF2I), Accession No. Hs 520623 (C7orf27), Accession No. Hs 520640 (ACTB), Accession No. Hs 520740 (SCRN1), Accession No. Hs 520794 (YKT6), Accession No. Hs 520898 (CTSB), Accession No. Hs 520943 (WBSCR1), Accession No. Hs 520967 (MDH2), Accession No. Hs 520973 (HSPB1), Accession No. Hs 520974 (YWHAG), Accession No. Hs 521064 (ZNF655), Accession No. Hs 521151 (FLJ22301), Accession No. Hs 521289 (REPIN1), Accession No. Hs 521487 (MGC8721), Accession No. Hs 521640 (RAD23B), Accession No. Hs 521809 (COBRA1), Accession No. Hs 521903 (LY6E), Accession No. Hs 521924 (SIAHBP1), Accession No. Hs 521969 (NDUFB11), Accession No. Hs 521973 (WDR13), Accession No. Hs 522074 (DSIPI), Accession No. Hs 522110 (CREB3), Accession No. Hs 522114 (CLTA), Accession No. Hs 522310 (NANS), Accession No. Hs 522373 (GSN), Accession No. Hs 522394 (HSPA5), Accession No. Hs 522463 (EEF1A1), Accession No. Hs 522507 (FBXW5), Accession No. Hs 522584 (TMSB4X), Accession No. Hs 522590 (EIF1AX), Accession No. Hs 522632 (TIMP1), Accession No. Hs 522665 (MAGED2), Accession No. Hs 522675 (FLJ12525), Accession No. Hs 522752 (PSMD10), Accession No. Hs 522817 (BCAP31), Accession No. Hs 522819 (IRAK1), Accession No. Hs 522823 (EMD), Accession No. Hs 522932 (NCOA4), Accession No. Hs 522995 (EIF4EBP2), Accession No. Hs 523004 (PSAP), Accession No. Hs 523012 (DDIT4), Accession No. Hs 523054 (SMP1), Accession No. Hs 523131 (TRAPPC3), Accession No. Hs 523145 (DDOST), Accession No. Hs 523215 (NDUFB8), Accession No. Hs 523238 (NOLC1), Accession No. Hs 523262 (C1orf8), Accession No. Hs 523299 (EIF3S10), Accession No. Hs 523302 (PRDX3), Accession No. Hs 523560 (HSPCA), Accession No. Hs 523680 (SSRP1), Accession No. Hs 523789 (TncRNA), Accession No. Hs 523829 (POLD4), Accession No. Hs 523836 (GSTP1), Accession No. Hs 523852 (CCND1), Accession No. Hs 523875 (INPPL1), Accession No. Hs 524009 (AASDHPPT), Accession No. Hs 524081 (DPAGT1), Accession No. Hs 524084 (RNF26), Accession No. Hs 524161 (RSU1), Accession No. Hs 524171 (RAD52), Accession No. Hs 524183 (FKBP4), Accession No. Hs 524195 (ARHGAP21), Accession No. Hs 524214 (MLF2), Accession No. Hs 524219 (TPI1), Accession No. Hs 524271 (PHC2), Accession No. Hs 524367 (CBARA1), Accession No. Hs 524395 (TUBA3), Accession No. Hs 524464 (ATP5G2), Accession No. Hs 524502 (RNF41), Accession No. Hs 524530 (CTDSP2), Accession No. Hs 524590 (RAB21), Accession No. Hs 524599 (NAP1L1), Accession No. Hs 524690 (PPIE), Accession No. Hs 524788 (RAB35), Accession No. Hs 524809 (RSN), Accession No. Hs 524899 (SAP18), Accession No. Hs 524920 (ZFP91), Accession No. Hs 524969 (Ufm1), Accession No. Hs 525134 (FLJ20277), Accession No. Hs 525163 (ANKRD10), Accession No. Hs 525232 (LRP10), Accession No. Hs 525238 (C14orf119), Accession No. Hs 525330 (ARF6), Accession No. Hs 525391 (FLJ20580), Accession No. Hs 525527 (RER1), Accession No. Hs 525626 (PACS1L), Accession No. Hs 525899 (C6orf49), Accession No. Hs 526464 (PML), Accession No. Hs 526521 (MDH1), Accession No. Hs 527105 (HNRPDL), Accession No. Hs 527193 (RPS23), Accession No. Hs 527348 (AKAP9), Accession No. Hs 527412 (ASAH1), Accession No. Hs 527861 (OS-9), Accession No. Hs 527862 (PKD1), Accession No. Hs 527980 (DUT), Accession No. Hs 528050 (HARS), Accession No. Hs 528222 (NDUFS4), Accession No. Hs 528300 (PITRM1), Accession No. Hs 528305 (DDX17), Accession No. Hs 528572 (SCAM-1), Accession No. Hs 528668 (RPL6), Accession No. Hs 528780 (GSPT1), Accession No. Hs 528803 (UQCRC2), Accession No. Hs 529059 (EIF3S4), Accession No. Hs 529132 (SEPW1), Accession No. Hs 529244 (NCK2), Accession No. Hs 529280 (ANAPC7), Accession No. Hs 529303 (ARPC2), Accession No. Hs 529369 (AFAP), Accession No. Hs 529400 (IFNAR1), Accession No. Hs 529420 (UBE2G2), Accession No. Hs 529591 (TLOC1), Accession No. Hs 529618 (TFRC), Accession No. Hs 529631 (RPL35A), Accession No. Hs 529782 (VCP), Accession No. Hs 529798 (BTF3), Accession No. Hs 529862 (CSNK1A1), Accession No. Hs 529890 (CANX), Accession No. Hs 529892 (SQSTM1), Accession No. Hs 529957 (SEC63), Accession No. Hs 530096 (EIF3S2), Accession No. Hs 530118 (LUC7L2), Accession No. Hs 530291 (ANXA11), Accession No. Hs 530314 (SSNA1), Accession No. Hs 530331 (PDHA1), Accession No. Hs 530381 (PIM3), Accession No. Hs 530412 (PAI-RBP1), Accession No. Hs 530436 (STXBP3), Accession No. Hs 530479 (PMF1), Accession No. Hs 530687 (RNH), Accession No. Hs 530734 (MRPL16), Accession No. Hs 530753 (FLJ20625), Accession No. Hs 530823 (COPS7A), Accession No. Hs 530862 (PRKAG1), Accession No. Hs 531081 (LGALS3), Accession No. Hs 531089 (PSMA3), Accession No. Hs 531176 (SARS), Accession No. Hs 531330 (CBWD1), Accession No. Hs 531614 (BTBD14B), Accession No. Hs 531752 (RANBP3), Accession No. Hs 531856 (GAS5), Accession No. Hs 531876 (DNCL2A), Accession No. Hs 531879 (RAD1), Accession No. Hs 532359 (RPL5), Accession No. Hs 532399 (KIAA0663), Accession No. Hs 532755 (GTL3), Accession No. Hs 532790 (NMT1), Accession No. Hs 532793 (KPNB1), Accession No. Hs 532803 (HN1), Accession No. Hs 532826 (MCL1), Accession No. Hs 532853 (NDUFB7), Accession No. Hs 533030 (HRIHFB2122), Accession No. Hs 533059 (TUBB), Accession No. Hs 533122 (SFRS10), Accession No. Hs 533136 (LRPAP1), Accession No. Hs 533192 (TOMM20), Accession No. Hs 533222 (HSA9761), Accession No. Hs 533245 (DDX46), Accession No. Hs 533282 (NONO), Accession No. Hs 533308 (PPP2R5D), Accession No. Hs 533317 (VIM), Accession No. Hs 533437 (TCEB1), Accession No. Hs 533440 (WWP1), Accession No. Hs 533474 (PPP1R8), Accession No. Hs 533479 (LYPLA2), Accession No. Hs 533526 (ATRX), Accession No. Hs 533624 (H3F3A), Accession No. Hs 533712 (RBM4), Accession No. Hs 533732 (SRP14), Accession No. Hs 533771 (STUB1), Accession No. Hs 533782 (KRT8), Accession No. Hs 533977 (TXNIP), Accession No. Hs 533985 (EXOC7), Accession No. Hs 533986 (ZNF258), Accession No. Hs 534125 (HLA-C), Accession No. Hs 534168 (NDUFA1), Accession No. Hs 534212 (SEC22L1), Accession No. Hs 534255 (B2M), Accession No. Hs 534307 (CCND3), Accession No. Hs 534314 (EIF5A), Accession No. Hs 534326 (ITGB4BP), Accession No. Hs 534338 (PPP4C), Accession No. Hs 534346 (RPS7), Accession No. Hs 534350 (SMARCB1), Accession No. Hs 534453 (GRIM19), Accession No. Hs 534456 (ANAPC11), Accession No. Hs 534457 (C14orf166), Accession No. Hs 534473 (TOMM22), Accession No. Hs 534483 (MGC2941), Accession No. Hs 536275 (PACS1), Accession No. Hs 541269 (NDUFB9), Accession No. Hs 546248 (CTSD), Accession No. Hs 546250 (DNCI2), Accession No. Hs 546253 (FDFT1), Accession No. Hs 546261 (HNRPA1), Accession No. Hs 546269 (RPL10A), Accession No. Hs 546271 (PCBP2), Accession No. Hs 546286 (RPS3), Accession No. Hs 546289 (RPS12), Accession No. Hs 546290 (RPS18), Accession No. Hs 546291 (NET-5), Accession No. Hs 546339 (SMAP), Accession No. Hs 546356 (RPL13A), Accession No. Hs 546394 (HSPC016), Accession No. Hs 547759 (SSBP3), Accession No. Hs 549178 (C9orf86), Accession No. Hs 552590 (HTF9C), Accession No. Hs 553496 (PGM3), Accession No. Hs 553512 (C3F), Accession No. Hs 554767 (NUP88), Accession No. Hs 554776 (SREBF1), Accession No. Hs 554894 (FLJ12953), Accession No. Hs 554896 (MGC11257), Accession No. Hs 555194 (FAM36A), Accession No. Hs 555866 (C1QBP), Accession No. Hs 555873 (HNRPAB), Accession No. Hs 555875 (IDH3A), Accession No. Hs 555889 (PSMC2), Accession No. Hs 555890 (RBBP4), Accession No. Hs 555911 (RBM8A), Accession No. Hs 555969 (RIC8), Accession No. Hs 555971 (PP1201), Accession No. Hs 555973 (MRPS25), Accession No. Hs 555994 (LONP), Accession No. Hs 556267 (FBXL10), Accession No. Hs 556461 (NDUFV2), Accession No. Hs 556795 (PAICS), Accession No. Hs 557550 (NPM1), Accession No. Hs 558296 (ACP1), Accession No. Hs 558313 (COX6A1), Accession No. Hs 558322 (EEF1B2), Accession No. Hs 558325 (EIF5), Accession No. Hs 558328 (FKBP5), Accession No. Hs 558330 (FTL), Accession No. Hs 558338 (HSPE1), Accession No. Hs 558345 (IK), Accession No. Hs 558354 (LAMR1), Accession No. Hs 558360 (NDUFB4), Accession No. Hs 558361 (NME2), Accession No. Hs 558362 (NUMA1), Accession No. Hs 558376 (RAC1), Accession No. Hs 558381 (RNU65), Accession No. Hs 558382 (RPL15), Accession No. Hs 558383 (RPL18A), Accession No. Hs 558384 (RPL19), Accession No. Hs 558385 (RPL23A), Accession No. Hs 558386 (RPL34), Accession No. Hs 558388 (RPS3A), Accession No. Hs 558389 (RPS8), Accession No. Hs 558390 (RPS24), Accession No. Hs 558391 (RPS26), Accession No. Hs 558396 (SCD), Accession No. Hs 558424 (CSDA), Accession No. Hs 558426 (EIF3S5), Accession No. Hs 558429 (G10), Accession No. Hs 558431 (RPL14), Accession No. Hs 558442 (PDCD6IP), Accession No. Hs 558448 (TXNL2), Accession No. Hs 558453 (ATP5L), Accession No. Hs 558454 (NUDC), Accession No. Hs 558458 (COPS8), Accession No. Hs 558473 (C18orf10), Accession No. Hs 558499 (8D6A), Accession No. Hs 558511 (PSARL), Accession No. Hs 558521 (C2orf33), Accession No. Hs 558591 (ORMDL1), Accession No. Hs 558825 (PDE4DIP), Accession No. Hs 558995, Accession No. Hs 567260 (CD2BP2), Accession No. Hs 567263 (C1orf43), Accession No. Hs 567267 (ATP2C1) and Accession No. And one or more genes selected from the group consisting of Hs 567279 (HCNGP).

The composition of claim 5, wherein the detection reagent is a primer pair and / or probe capable of specifically amplifying a candidate standard expression gene.

The composition of claim 7, wherein the primer pairs are 10-50 bp long sense primers selected from the sense sequences of the genes and 10-50 bp long antisense primers selected from the antisense sequences of the genes.

The composition of claim 8, wherein the length of the sense primer is 12 to 30 bp.

The composition of claim 8, wherein the antisense primer is 12 to 30 bp in length.

6. The composition of claim 5, wherein the gene detection is measured by microarray, Serial Analysis of Gene Expression (SAGE) or quantitative real time PCR (qRT-PCR) method.

Selecting a reference gene when measuring the gene expression amount comprising measuring the coefficient of variation (CV) of the candidate standard expression gene selected by the method of claim 1 and selecting the standard expression gene in the order of low coefficient of variation Way.

The method of claim 12, wherein the reference gene is a gene expressed in most tissues of the human body.

The method according to claim 12, wherein the reference gene can be used for standardization of the expression level between samples in the relative quantitative analysis of the gene expression level.

13. The method of claim 12, wherein the coefficient of variation is larger as the change in gene expression amount increases.

A composition for detecting the reference gene, comprising a detection reagent capable of detecting the reference gene selected by the method of claim 12.

The method of claim 16, wherein the reference gene is Accession No. Hs 500775 (ZNF207), Accession No. Hs 446427 (OAZ1), Accession No. Hs 530118 (LUC7L2), Accession No. Hs 208597 (CTBP1), Accession No. Hs 440382 (TRIM27), Accession No. Hs 444279 (GPBP1), Accession No. Hs 250009 (ARL8B), Accession No. Hs 9589 (UBQLN1), Accession No. Hs 253726 (PAPOLA), Accession No. Hs 146806 (CUL1), Accession No. Hs 533222 (DIMT1L), Accession No. Hs 494985 (FBXW2) and Accession No. And one or more genes selected from the group consisting of Hs 242458 (SPG21).

17. The composition of claim 16, wherein the detection reagent is a primer pair and / or probe capable of specifically amplifying a reference gene.

The composition of claim 16, wherein the reference gene exhibits an expression level similar to the low expression level of most transcripts present in the cell.

19. The composition of claim 18, wherein the primer pairs are 10-50 bp long sense primers selected from the sense sequences of the genes and 10-50 bp long antisense primers selected from the antisense sequences of the genes.

The composition of claim 20, wherein the length of the sense primer is 12 to 30 bp.

The composition of claim 20, wherein the antisense primer is 12 to 30 bp in length.

21. The composition of claim 20, wherein the gene detection is measured by microarray, Serial Analysis of Gene Expression (SAGE) or quantitative real time PCR (qRT-PCR) method.

1) obtaining cDNA from RNA obtained from the subject;

2) amplifying the target gene by real-time PCR using primer pairs and / or probes capable of amplifying the target gene using the cDNA of step 1) as a template DNA, and using the composition of claim 5 Amplifying the candidate standard expression gene by real-time PCR; And,

3) A method of quantifying the expression level of the target gene comprising the step of normalizing the expression level of the target gene of step 2) and the expression level of the candidate standard expression gene.

1) obtaining cDNA from RNA obtained from the subject;

2) amplifying the target gene by real-time PCR using a primer pair and / or a probe capable of amplifying the target gene using the cDNA of step 1) as a template DNA, and using the composition of claim 16 in the sample. Amplifying the candidate standard expression gene by real-time PCR; And,

1) Amplifying the target gene by real-time PCR using primer pairs and / or probes capable of amplifying the target gene using the genomic DNA obtained from the subject as a template DNA, and including it in the sample using the composition of claim 5 Amplifying the candidate standard expressing gene by real-time PCR; And,

2) A method for discriminating amplification in genomic DNA of a target gene comprising the step of normalizing the expression level of the target gene of step 1) and the expression level of the candidate standard expression gene.

1) Amplifying the target gene by real-time PCR using primer pairs and / or probes capable of amplifying the target gene using genomic DNA obtained from the subject as a template DNA, and including it in the sample using the composition of claim 16. Amplifying the candidate standard expressing gene by real-time PCR; And,

2) Amplification and discrimination method in the genomic DNA of the gene to be quantified, comprising the step of standardizing the expression level of the target gene and the candidate standard expression gene of step 1).