KR20090073954A - A method to produce a spirulina algae using deep sea water - Google Patents

Abstract

A method for producing a spirulina algae using deep sea water is provided to massively produce the spirulina algae using concentrated salt water as a medium for spirulina algae. A method for producing a spirulina algae using deep sea water comprise: a step of collecting deep sea water and preparing concentrated salt water; a step of culturing the spirulina algae in the concentrated water; and a step of producing spirulina algae product from the cultured spirulina algae. In the process for culturing the spirulina algae, the sunlight and 2000-6500Lux of light are used in the daytime and in the night.

Description

A method to produce a spirulina algae using deep sea water}

The present invention relates to a method for the production of spirulina algae, more specifically, the brine concentrated in the reverse osmosis membrane while producing a drinking water and the like by taking deep sea water deeper than 200m deep from the sea surface The present invention relates to a method for producing spirulina or algae at low cost industrially and efficiently throughout the year through day and night without being affected by the culture site in large quantities.

In conventional algae culture, sunlight has been cultivated as a light source among the cultures installed outdoors, and in the case of producing algae with sunlight as a light source among the cultures installed outdoors, the amount of irradiation light fluctuates due to factors such as season, climate, day and night, and region. Since the fluctuation of the algae yield is severe with the fluctuation of these factors, there was a problem that a large amount of algae cannot be cultured at low cost industrially and efficiently throughout the year through day and night without being affected by the culture site.

In addition, the culture of Spirulina algae, such as Spirulina Platensis, is a modification of the composition of the SOT medium developed by Mr. Ogawa Takahira, et al. In the case of 5, there was a problem in that the production cost was high because the artificial growth medium had to be supplied at a lower specific growth rate (also referred to as the non-growth rate) than when the deep ocean water was used as a medium. In the case of 7, the method of culturing the deep seawater mixed with the freshwater or the deep seawater as a medium also had a problem that the specific growth rate was lower than that when the concentrated deep seawater was concentrated.

Literature Information of the Prior Art

[Document 1] Republic of Korea Patent Registration No. 10-0564109 (2006 03. 20)

[Document 2] Korean Patent Registration No. 10-0622025 (2006 09. 01)

[Document 3] Korean Patent Registration No. 10-0697610 (2007.03.14)

[Document 4] Korean Patent Registration No. 10-0704436 (2007. 03. 30)

[Patent 5] Japanese Patent Application Laid-open No. 5-184347 (July 27, 1993)

[Document 6] Japanese Patent Publication No. 2002-262858 (September 17, 2002)

[Document 7] Japanese Patent Publication No. 2002-315568 (October 29, 2002)

In order to solve the above problems, intake of the deep sea water of the seabed deeper than 200m deep in the sea level with a large amount of nutrients required for the growth of algae to prepare a drink, salt with concentrated brine concentrated in the reverse osmosis membrane In the process of producing the spirulina algae (Spirulina algae) to provide a method for producing a low cost efficiently.

In the present invention, in the production of spirulina algae (Spirulina algae), taking the deep sea water of the seabed deeper (sea 底 層) deeper than 200 m from the sea surface to produce concentrated brine, spirulina algae using the concentrated brine as a culture medium The step of culturing, characterized in that consisting of producing a spirulina algae product from the cultured spirulina algae.

The present invention is a hygienic safe and continuous by using the concentrated brine as a culture medium of spirulina or algae in the process of producing a drinking water, the brine is concentrated and brine unfiltered to produce a drinking water by taking the deep sea water reverse osmosis Because of its high efficiency and low-cost cultivation, it is expected to have an effect widely used in the production of spirulina and algae.

First, when the characteristics of deep ocean water are reviewed, the deep ocean water contains various minerals necessary for the growth of animals and plants, as shown in the following Table 1 "Analysis of Significant Values in Ocean Deep Water and Superficial Sea Water" Is contained in extremely small amounts, and has a low concentration of harmful microorganisms and pollutants, and a high concentration of nutrients necessary for the growth of algae.

Table 1 Analysis of Significant Values in Deep-sea and Superficial Seawater

division Ulleungdo Hyunpo Murodo, Koji Prefecture, Japan 650m deep sea water Surface waters 374m deep sea water Surface waters   General Item Water temperature (℃)  0.5 23 11.5 20.3 pH 7.8 8.15 7.8 8.15 DO dissolved oxygen (mg / l) 6 8 7.80 8.91 TOC Organic Carbon (mg / L) 0.6  - 0.962 1.780 COD _Mn (mg / L) 0.2 0.6  -  - Soluble eluent residue (mg / l) - - 47,750 37,590 M-alkalido (mg / l) - - 114.7 110.5    Main element Cl chloride ion (wt%) 2.69 with NaCl 2.75 with NaCl 2.237 2.192 Na sodium (wt%) 1.080 1.030 Mg magnesium (mg / l) 1,270 1,280 1,290 1,310 Ca Calcium (mg / L) 406 405 402 403 K potassium (mg / L) 380 - 414 399 Br Clear (mg / L) 68.2 - 68.8 68.1 Sr Strontium (mg / L) 7.76 - 7.77 7.61 B boron (mg / l) 4.45 - 4.44 4.48 Ba barium (mg / l) 0.007 - 0.044 0.025 F Fluorine (mg / L) 0.52 - 0.53 0.56 ^{_{SO 4 2 - (㎎ / ℓ}} ) 2,836 - 2,810 2,627  Nutrient salts NH ₄ ⁺ ammonia nitrogen (mg / l) 0.05 - 0.05 0.03 NO ₃ ^- Nitrogen Nitrate (mg / l) 0.28 0.04 1.158 0.081 PO ₄ ^{3 -} phosphate (mg / l) 0.06 0.012 0177 0.028 Si silicon (mg / l) 2.8 0.44 1.89 0.32   Trace element Pb lead (μg / ℓ) 0.11 - 0.102 0.087 Cd cadmium (㎍ / ℓ) 0.05 - 0.028 0.008 Cu copper (㎍ / ℓ) 0.26 - 0.153 0.272 Fe iron (㎍ / ℓ) 0.23 - 0.217 0.355 Mn manganese (µg / l) 0.26 - 0.265 0.313 Ni nickel (µg / l) 0.36 - 0.387 0.496 Zn zinc (μg / ℓ) 0.45 - 0.624 0.452 As Arsenic (㎍ / ℓ) 0.04 - 1.051 0.440 Mo molybdenum (µg / l) 5.11 - 5.095 5.565 Cr chromium (µg / l) 0.02 - - - Number of bacteria Number of live bacteria (dog / ml) 0 520 0 540 E. coli count (pcs / ml) voice voice voice voice

Although the history of deep sea water use is very short, various researches have been conducted in the non-fishery fields such as food, medical, health industry, beverages, and cosmetics, starting with the fisheries field.

Deep sea water is usually called deep sea water that is deeper than 200m deep, and unlike surface sea water, it is not exposed to sunlight, so plankton and life cannot grow, so nutrients (榮 養 鹽) High concentration of 농도) and density difference according to water temperature, so there is no contaminant in surface seawater because it is not mixed with surface seawater. There are characteristics such as stability, cleanliness, eutrophicity, mineral balance characteristics, and maturation characteristics.

1. Low temperature safety

While the surface temperature of surface seawater fluctuates greatly with the seasons, deep ocean waters are stable at low temperatures without changing the water temperature with the seasons.

In 1968, the US Navy submerged Armin sank in the deep sea and after about a year and a half, sandwiches were left intact, intact, and the eggs were in high pressure. The salt and minerals infiltrated into the protein changed the elasticity and the elasticity, but it was not deteriorated at all. Usually, the sandwich would rot even if only one week was put in the refrigerator at the same temperature as the deep sea. This is because the growth of microorganisms was suppressed in the state of low temperature and high pressure.

In particular, deep sea waters in the East Sea of Korea settled by dense differences in cold seawater that melted the drift ice in the Sea of Okhotsk, off the Vladivostok between Ostrov Sakhalin and Hokkaido. As the deep water flows in and blocks the Japanese archipelago, it slows down the flow. At deep seabeds that are deeper than 300m above sea level, the water temperature is 1 ~ 2 ℃ throughout the year, and the waters off Muroto in Kouchi Prefecture, Hawaii or Japan Compared with deep ocean water, it has a characteristic of about 8 to 11 ° C. lower.

2. Cleanliness

Because it is deep in the seabed, it is difficult to receive pollution from river water and air on land, and there are few chemicals, pollutants and bacteria.

① physical cleanliness

Physical cleanliness is said to be less suspended suspended matter, and deep sea water contains less suspended solids than surface seawater.

② biological cleanliness

The biggest problem in the intake of seawater is the propagation of adherent organisms. In general, in surface water intake systems, the adherent organisms propagate in the intake pipe, and the resistance of the pipe increases and it becomes impossible to take in water. The total number of live bacteria such as microorganisms, chlorella, etc. is characterized by a small amount from one tenth to one hundredth of the surface number.

③ chemical cleanliness

Since deep seawater does not mix with contaminated surface waters, it is not contaminated with so-called environmental pollutants such as dioxins, PCBs, organic chlorine compounds, and organic tin.

3. eutrophicity

Deep sea water is about 5 to 10 times higher than that of surface seawater compared to surface seawater. Nitrogen, phosphorus and silicic acid, which are the nutrient sources of algae and phytoplankton (mainly diatoms of microorganisms with chlorophyll) Inorganic nutrients are abundantly contained.

At sea levels deeper than 150 m above sea level, the amount of light is less than 1%, and at further depths, phytoplankton are unable to photosynthesize, so nutrients are not consumed by phytoplankton and are accumulated and accumulated in the lower layers. High concentration of nutrients

4. Characteristics of minerals

Sea water contains more than 70 kinds of elements, and deep sea water has such characteristics that it contains various kinds of elements.

Although there are many important elements necessary for the growth of animals and plants, they are necessary, but mineral traces that contain very small amounts of deeply related to human health, such as copper and zinc, essential trace elements that are harmful to intake in large quantities. There is a good character.

5. Aging

Deep sea water has a lower pH than surface sea water (around 7.8), and the organic content is low, while deep sea water is separated from surface sea water, and small clusters of water molecules are kept for a long time under low pressure and high pressure. Micro-clustered water, which is liquefied, has stable water quality.

Microorganisms, which play a major role in the fermentation and ripening of foods, are characterized by cell membranes and many minerals in the cells. These microorganisms have an active metabolic activity when enough minerals are supplied. It inhibits the growth of harmful microorganisms in (相互 拮抗 關係).

The production of useful microorganisms in water with sufficient minerals is called the BMW System (Bacteria-Mineral-Water System).

The present invention proposes a method of culturing spirulina or algae using nutrient-rich marine deep water in a seabed deeper than 200m deep from the sea level using the above-described principle.

Water molecules form clusters by hydrogen bonding, and the method of measuring the number of such water molecule populations is currently known as Nuclear magnetic resonance (NMR). ¹⁷ O-NMR Spectrum half-width of () is estimated indirectly, and approximately 1/10 of the nuclear magnetic resonance ¹⁷ O-NMR Half-width (폭) of the water molecules It is known as the number of groups.

When the hydrogen bonds of water molecules are partially cleaved by small cleavage, the surface tension decreases, the permeability is improved, and the taste is improved. The same treatment is sometimes referred to as "aging."

In general, when produced from river water, the nuclear magnetic resonance ¹⁷ O-NMR half-value width is 130 to 150 Hz, and 13 to 15 water molecules form an aggregate. Such water is called bound water. In the case of deep ocean water, there are significant differences depending on the location. For deep ocean water taken at 1,400 meters off the coast of Urasoe City, Okinawa, Japan, the value of the nuclear magnetic resonance ¹⁷ O-NMR half-width Was 78㎐, and the deep seawater taken from Ulleungdo's Hyeonpo 650m was 65.5㎐. As such, water having a small value of the nuclear magnetic resonance ¹⁷ O-NMR half width is referred to as microclustered water.

In the present invention, the measurement of the specific gravity of the solution to determine the concentration of salinity is measured by Baume's hydrometer, and the Baumedo (° Be) of the Baume hydrometer is used to measure the specific gravity of the liquid. It is a numerical value of the scale when it floats, and heavy bomedo for heavy liquids that is heavier than water specific gravity, and light bomedo for hard liquids that is lighter than specific gravity of water. Among them, the heavy liquid is 0 ° Be of pure water, the 15% saline is 15 ° Be, and the division is divided into 15 equal parts, and the hard solution is 0 ° Be of 10% saline. The pure water is 10 ° Be, and the division is divided into 15 equal parts.Bomedo (° Be) is a salt because it approximates the salt concentration (wt%) in seawater. It is also widely used as a measure of concentration.

The relationship between the Bume (° Be) and the specific gravity (d) of the liquid is

For heavy media that has a specific gravity of liquid greater than that of water

d = 144.3 / (144.3- ° Be). … … … … … … … … … … … … … … … … … ①

In the case of an alarm field where the specific gravity of the liquid is lower than the specific gravity of the water

d = 144.3 / (134.3 + ° Be). … … … … … … … … … … … … … … … … … ②

Spirulina is a Latin word meaning “Spira” in Latin language. Spirulina is characterized by its propagation under the special environment of strong alkali, high temperature salt lake. It grows in alkaline salt lakes such as Lake Chad in Africa, Lake Nakuru, and Lake Texcoco in Mexico. Chlorella) and others are attracting attention as a future protein source.

Spirulina is a turquoise spiral, 300-500 µm long, 8 µm long, Spirulina abbreviata, Spirulina adriatica, Spirulina agilis, Spirulina azilisshima agilissima, Spirulina albida, Spirulina ardissoni, Spirulina breviaticulata, Spirulina corakiana, Spirulina corruana Spirulina cabrida (Spirulina caldaria), Spirulina cavanillesiana, Spirulina crispum, Spirulina curta, Spirulina flavovirens, Spirulina flapis Spirulina geitleri, Spirulina zicantia lina gigantea, Spirulina gomontiana, Spirulina gomontii, Spirulina gordiana, Spirulina jenneri, Spirulina igneri, Spirulina ipirina Spirulina labyrinthiformis, Spirulina laxa, Spirulina laxissima, Spirulina maiol, Spirulina major, Spirulina margarita Spirulina margarita ), Spirulina maxima, Spirulina mediterranea, Spirulina meneghiniana, Spirulina miniata, Spirulina nordsteadi (Spirulina nordstedi) okensis, Spirulina platensis, Spirulina princeps, Spirulina pseudovacuolata, Spirulina regis, Spirulina rosea, Spirulina rosero, Spirulina schroederi, Spirulina schroederi siamese, Spirulina spirulinoides, Spirulina subsalsa, Spirulina subtilissima, Spirulina tenerrima, Spirulina tenru, or Spirulina (Spirulina tenuis), Spirulina tenerrima, Spirulina tenuissima, Spirulina undulans, Spirulina versicolor, Spirulina versiru and wexi Of cyanobacteria, Blue-green algae It breeds in seawater and hot brine of tropical region with high salinity and strong alkalinity. The composition is 60 ~ 70% of protein, 6 ~ 9% of lipid and 15 ~ 20 of carbohydrate. It is composed of%, contains vitamins, minerals (Mineral), cellulose (Cellulose), etc., contains carotenoids (Carotinoid), chlorophyll (Chlorophyll), phycocyanin (Phycocyan). It contains all the essential amino acids and is also rich in essential fatty acids, linolenic acid and gamma linolenic acid. In addition, digestive absorption rate is more than 95% is characterized by good digestion.

Phycocyanin, a spirulina color derived from spirulina, is a non-tar-based natural pigment and is used in many fields such as ice cream, confectionery, and dairy products.

The inventors of the present invention are marine that the intake Sim stories by the heating treatment with the microfiltration pretreatment (Micro-filtration) nanofiltration (Nanofiltration) sulfate ion (SO ₄ ^{2 -)} in the removal of the then reverse tuyeogwa (Reverse osmosis The filtrate filtered in the process is a beverage in the beverage manufacturing process, and the concentrated brine concentrated without filtration is evaporated from the salt in Cheonil, and the salt is precipitated to produce salt. Attention is drawn to the spontaneous growth of spirulina algae and the spontaneous growth of spirulina algae in the evaporation zone with a water temperature of 25 to 35 ° C and a specific gravity of 8 to 18 ° Be. As a result of observation, it was confirmed that the spirulina or algae can be efficiently produced, and the present invention will be described in detail with reference to the accompanying drawings.

I. Steps to produce concentrated brine

1. Intake and warming process

In the pretreatment process, the deep sea water of the seabed deeper than 200m deep from the sea surface is taken out and warmed so that the subsequent treatment can be smoothly processed.

In FIG. 1, the deep sea water intakes deep sea water from a deep seabed deeper than 200 m from the sea surface, and the intake method is to take down pipes from a ship to a depth of 200 m deep, or to a deep sea bed deeper than 200 m deep from sea level. The pipe is installed to take water with a pump, or the pipe is installed from the sea level to the sea floor deeper than 200m deep, and the intake well is installed below the sea level to take water according to the siphon principle.

The deep sea water collected in the sump is low in temperature and high in viscosity, and the treatment efficiency is low, so it is supplied with heat from a boiler (in summer, the surface water temperature may be used), and then warmed to 20 to 30 ° C to filter the pretreatment. Send to fair

2. Pretreatment Filtration Process

The pretreatment filtration process removes suspended solids (SS) by filtration through sand filtration, micro filtration or ultra filtration alone or a combination of two or more processes. Next, it is sent to the nanofiltration process.

At this time, the filtration pressure is determined in consideration of the pressure loss of the filter and the pressure loss of the pipe according to the operating conditions.In the case of sand filtration, the filtration speed is 6-10 m / hour, and the effective diameter of the filter sand Is 0.3 to 0.45 mm, the uniformity factor is 2.0 or less, and the thickness of a fibrous layer is 0.5 to 1.0 m.

At this time, if the turbidity of the deep ocean water taken is 2 mg / ℓ or less, it is not necessary to sand filtration.

Micro-filter and ultra-filter are not limited to the type of filtration membrane, and the supply pressure of the pump is decided by considering the filtration speed and the pressure loss according to the vendor's specifications. do.

In microfiltration or ultrafiltration, filtration treats the water's fouling index (FI) in the range of 2-4.

The FI value is a numerical value representing the fine turbidity concentration in the target water.

FI = (1-T ₀ / T ₁₅ ) x 100/15... … … … … … … … … … … … … … … … … … … … ①

Where T ₀ is the time required for filtration of the first 500 ml of sample water when the sample water was filtered under pressure of 0.2 MPa using a 0.45 μm microfiltration membrane, and T ₁₅ was filtered for 15 minutes in the same state as T _0. It is time required for filtration of 500 ml of sample water after that.

2. Nano filtration process

Nanofiltration process, the sulfate ions that cause scale (Scale) generated in the reverse tuyeogwa of the post processing (SO ₄ ^{2 -)} as is the main purpose to remove, deep ocean water, removing the suspended solids in the water in the pre-filtering step is a nanofiltration Sulfate-containing water that has not been sent to the process is discharged, and desulfurized ion mineral salt, which is filtered water, is sent to the reverse osmosis filtration process.

Transmission sequence of the ion in the nanofiltration membrane, if the cation is ^{Ca 2 +> Mg 2 +>} Li +> Na +> K +> and NH ₄ ^+, if the anion is _{^{SO 4 2 - »HCO 3 ->}} F - ^{> Cl -> Br -> NO} 3 -> and SiO _2, a sulfate ion (SO ₄ ^{2 -),} if the it is difficult to permeate than Mg ^{+ 2} and Ca ^{+ 2.}

In the nanofiltration process, the supply pressure is 15 to 20 kg / cm 2, which is lower than the osmotic pressure of 25 kg / cm 2 of the deep seawater with a salt concentration of 3.5 wt%. In the case of spiral, the membrane permeability is 0.7 to 1.4 m 3 / If m 2 · day, the membrane permeate amount is 70 to 80% of the inflow rate.

Example 1

After warming the deep seawater as shown in Table 1 at 25 ° C and pretreating the FI value to 3.2 by ultrafiltration, it is a cross-linked polyamide material manufactured by Toray Industries, Ltd. The membrane permeate was 80% of the influent when the pressure was supplied to the membrane at 20 kg / cm 2 G using the spiral nanofiltration membrane of SU-610, and the membrane permeate was 1.2 m 3 / m 2 · day. Analysis of the main components of the non-sulfate-ion-containing water and the filtered desulfurized ionmine water is shown in Table 2.

Table 2 Analysis of Principal Components of Unfiltered Sulfate-Ion Water and Filtrated Desulphate-ion Mineral Saline by Nanofiltration

 Item      Pretreated Marine Deep Water (Raw Water)         Filtrated Desulphate Ion Mineral Saline    Unfiltered sulfate-containing water      pH        7.80         7.24          7.82 Na ⁺ (mg / L)   10,800    9,650     15,400 Cl ^- (㎎ / ℓ)   22,370   17,300     42,650 Ca ^{2 +} (㎎ / ℓ)      456      338        928 Mg ^{2 +} (㎎ / ℓ)    1,300    1,060      2,260 K ⁺ (mg / L)      414      355        650 _{^{SO 4 2 - (㎎ / ℓ}} )    2,833      319     12,890

As shown in Table 2, as a result of nanofiltration of deep sea water, Na was removed by 28.5%, calcium (Ca), 41%, and magnesium (Mg) by 35%, but more than 90% of sulfate ions were removed.

3. Reverse osmosis filtration process

When the filtered water from which sulfate ions have been removed in the nanofiltration process is supplied to the reverse osmosis filtration process, the filtered water, which is the demineralized water filtered by supplying the operating pressure to the reverse osmosis membrane at 50 to 70 kg / cm 2, is sent to the beverage production process and concentrated. Concentrated brine is sent to a concentrated brine reservoir (1).

In case of reverse filtration membrane of reverse osmosis filtration process, when operating pressure is 55 ~ 56㎏ / ㎠ and membrane permeate amount is 0.5 ~ 0.8㎥ / ㎡ · day, the salinity of filtered water is 99.0 ~ 99.85wt%. The salt contained in the deep sea water is concentrated while 40 to 60% of the influent is filtered.

The membrane module of the nanofiltration and the reverse osmosis (Module) form is tubular (tubular), hollow fiber (hollow fiber; hollow spiral), spiral (螺旋 形), flat plate (平板 形) Any form such as a frame may be used, and the material of the film is not particularly limited.

As the material of the nanofiltration membrane, polyamide-based, polypiperazineamide-based, polyesteramide-based, or crosslinked water-soluble vinyl polymer can be used. The membrane structure has a dense layer on one side of the membrane, and is an asymmetric membrane composed of micropores gradually from a large pore toward the inside of the membrane or toward one side of the membrane. A composite membrane having a very thin separation functional layer formed of another material on the dense layer of the membrane can be used, and a piperazine polyamide composite membrane is preferable, but in the present invention, the material and structure of the membrane It is not limiting.

Example 2

The filtrate filtered by the nanofiltration of Example 1 was a crosslinked polyamide-based composite membrane model number SU-810 in a high pressure reverse osmosis membrane of Toray Industries, Ltd., Japan. The membrane permeate was 52% of the influent when the pressure was fed to the membrane at 60 kg / cm 2 G using a spiral reverse osmosis membrane of. Analysis of the main components of the concentrated brine without concentration is shown in Table 3 below.

Table 3 Analysis of Major Components of Filtrate and Unfiltered Concentrated Saline in Reverse Osmosis

Item Influent Filtered filtrate Concentrated brine pH 7.24 7.20 7.28 Na ⁺ (mg / L) 9,650 38.7 20,063 Cl ^- (㎎ / ℓ) 17,300 71.6 35,478 Ca ^{2 +} (㎎ / ℓ) 338 0.6 703 Mg ^{2 +} (㎎ / ℓ) 1,060 1.9 2,206 K ⁺ (mg / L) 355 1.7 737 _{^{SO 4 2 - (㎎ / ℓ}} ) 319 3.7 1,584

As shown in Table 3, most of the substances removed by deep osmosis from deep ocean water were over 99%.

II. Incubating Spirulina algae

1. Magnetization process

When the concentrated brine concentrated without filtration in the reverse osmosis filtration process is supplied to the concentrated brine storage tank (1), the ferrous quaternary oxide (Fe) magnetized with a magnetic flux density of 100 to 400 gauss (Fe) ₃ O ₄₎ or magnetite (磁鐵鑛) hydrochloric acid in a powder 3~10wt% (prepared by dissolving HCl) aqueous solution of a divalent, trivalent, and a total iron concentration supplied to 3~100㎎ / ℓ range of iron salts in the concentrated brine, brine and concentrated The transfer pump (2) is sent to a magnetizer (3) installed on the discharge side for magnetization, and then sent to the carbon dioxide dissolution, heating and pH regulator (6).

However, when producing spirulina or algae having a low iron concentration, supplying the divalent and trivalent iron salts is omitted, and the concentrated brine is magnetized.

The magnetizer 3 uses one of a constant voltage conductor tube magnetizer and a permanent magnet, and the constant voltage conductor tube magnetizer is made of synthetic resin (PVC, PE, styrene resin, etc.), ebonite, FRP. When a low voltage of AC or DC in the range of 0.5 to 5 Volts is applied to a coil wound on a cylindrical tube of an insulating material such as Bakelite, a magnetic field is formed inside the coil. Instead of the constant voltage conductive tube magnetizer, permanent magnets magnetized in the range of 10,000 to 15,000 G (Gauss) are used.

The flow rate of supplying the concentrated brine of the concentrated brine storage tank 1 to the magnetizer 3 installed on the discharge side of the concentrated brine transfer pump 2 has a flow rate of 2 in the magnetic field inside the magnetizer 3. The magnetized treated brine is dissolved in carbon dioxide gas, heated, and sent to the pH regulator 6, while the concentrated brine treated by magnetization is returned to the concentrated brine reservoir 1 so that the range of -10 m / sec is maintained.

2. Carbonic acid gas melting, heating and pH adjustment process

When the magnetized concentrated brine is dissolved in carbon dioxide gas, heated and pH regulator 6, hot water or steam is supplied from the boiler while stirring with a stirrer 7 to adjust the temperature of the brine water temperature control controller (TIC). : The temperature indicating controller is used to control the temperature in the range of 25 ~ 42 ℃ and remove the fine dust from the boiler exhaust gas containing carbon dioxide (CO ₂ ) with a bag filter or electric precipitator. Carbon dioxide gas (CO ₂ ) capacity per volume of concentrated brine in the range of 0.03 to 0.08 vol% is blown into the blower (5) to improve the dissolution efficiency of carbon dioxide while supplying carbon dioxide to the bottom of the pH regulator (6). In order to dissolve the carbon dioxide gas, the carbon dioxide gas is dissolved in concentrated brine while the carbon dioxide is dissolved, heated and the internal pressure of the pH regulator 6 is adjusted to a range of 0.3 to 0.5 kg / cm 2 G by a pressure indicating controller (PIC). In addition, one type of alkaline aqueous solution of sodium hydroxide (NaOH), potassium hydroxide (KOH) or calcium hydroxide (Ca (OH) ₂ ) is supplied to a pH range of 9 to 11 by a pH indicating controller (pHIC). Then, it is sent to the algae culture tank (11).

The carbon dioxide melting, heating and pH regulator 6 is made of titanium, bronze, or flame resistant stainless steel. The carbon dioxide melting, heating and pH regulator 6 The heating jacket (Heating jacket, 8) is provided on the outside, the heating jacket (8) outside the heat insulating material (9).

The stirrer 7 is a propeller type, and the stirring speed is 180 to 360 rpm, and the size of the propeller is such that the ratio of the supply flow rate (Q) / capacity (V) is 1 to 4, and the material is heated. And one of titanium, bronze, or flame resistant stainless steel, such as the pH regulator 6.

In this case, the reaction between NaOH and carbon dioxide (CO ₂ ) as an alkali agent produces carbonate as follows.

NaOH + CO ₂ → NaHCO ₃ … … … … … … … … … … … … … … … … … … … ②

NaHCO ₃ + NaOH ↔ Na ₂ CO ₃ + H ₂ O. … … … … … … … … … … … … … … ③

3. Cultivation process of spirulina algae

When carbonic acid gas melting, heating and pH-adjusted concentrated brine are supplied to the algae culture tank 11 from the carbon dioxide gas melting, heating and pH regulator 6, spirulina algae by daylight 12 On the day when the weather is cloudy or at night, the amount of irradiation light 2000-6500 Lux (Lux) is irradiated with light from the back 13 of the spirulina or algae and sent to the algae settling tank (15).

Spirulina algae cultured in the algae culture tank (11) is supplied to the algae settling tank (15), when settled down the algae settling tank (15) boils to the bottom of the condensate tank (16) to the bottom (Cone) to collect the return pump (17) The cultured spirulina algae, which is a surplus alga, are sent to the surplus algae concentration tank 20 of the dehydration step while being returned to the algae culture tank 11 for the algae.

The concentrated brine overflowing to the upper part of the algae settling tank 15 is sent to the brine storage tank 18 together with the dehydration filtrate discharged from the dehydration process, followed by the salt production process by the brine transfer pump 19. Send to.

The flow rate of returning the algae precipitated in the algae settling tank 15 to the top of the algae culture tank 11 for the seeding algae by the conveying pump 17 is MLSS (Mixed liquor) of the culture solution of the algae culture tank 11. It is returned to maintain the concentration of suspended solids in the range of 2,500 to 3,500 mg / L.

The structure of the algae culture tank 11 is piled with soil, such as evaporation of salt salt, and the bottom is made of clay chopped by the clay to be waterproof, or a rectangular concrete (Concrete) structure. In addition, the depth of the algae culture tank 11 is in the range of 10 cm to 5 m, the capacity of the tank is to be a residence time in the range of 1 to 10 days.

The algae culture tank 11 is provided with one type of transparent dome 14 made of vinyl or glass, through which sunlight is easily transmitted, so that foreign matter does not flow in the rain without rain flowing in during rain. do.

The algae sedimentation tank 15 is a salt-resistant algae sedimentation tank lake that can boil and collect spirulina or algae that have settled inside the circular concrete structure to the lower cone part, as in the final sedimentation tank of the standard activated sludge treatment process of sewage and wastewater. 16), the surface area of the bath is determined by the cross-flow area in the range of overflow load of 16-30m 3 / m 2 · day, the depth of the tank is 2 ~ 4m, the slope of the bottom bottom The range of 1/10 to 2/10 is set, and the rotational speed of the algae settling tank 16 is set to 0.02 to 0.05 rpm.

When the diameter of the algae sedimentation tank 15 is 3 m or less, the algae sedimentation tank rake 16 is omitted, and the inclined surface of the bottom bottom is 45 to 60 degrees.

The carbon source for Spirulina algae is acetic acid, citric acid, tartaric acid, palmitic acid, oxalic acid, malic acid. , Succinic acid, Maleic acid, Male acid, Oxalic acid, Butyric acid, Fumaric acid, Formic acid, Picric acid, Picric acid, Ascorbic acid organic acids such as acid) or mixtures of one or more of these organic acid salts may be used in place of carbon dioxide gas or may be used together with carbon dioxide gas.

III. Steps to produce spirulina algae

1. Concentration and Primary Dewatering Process of Cultured Spirulina Algae

When the cultured spirulina alga, which is the excess algae, is supplied to the surplus algae concentration tank 20 of the dehydration process, the supernatant flowing over the surplus algae concentration tank 20 is sent to the brine storage tank 18, and the brine is transported. The pump (19) is sent to the salt manufacturing process, and the concentrated algae precipitated under the surplus algae concentration tank (20) is sent to the dehydration tank transfer pump (22) to the dehydrator to remove the algae dehydrated in the water content range of 75 to 82 wt%. Send to the second dehydration process.

The structure and material of the surplus algae thickening tank 20 are the same as those of the algae settling tank 15, and the surface area of the tank determines the cross-sectional area in the range of 60 to 90 kg / m 2 · solid load, and the depth of the tank is 3 to 4m.

The dehydrator uses one type of centrifugal dehydrator, roll belt press dehydrator, filter press dehydrator, vacuum dehydrator or screw press dehydrator. do.

2. Cleaning and Secondary Dewatering Process

The concentrated algae of the cultured spirulina algae and the dehydrated algae in the first dehydration process is injected with 10 to 20 kg of fresh water per kilogram of dehydrated algae because the salt concentration is high, and then the same dehydrator as the primary dehydrator Dehydrated algae with water content ranging from 75 to 82 wt% is sent to the drying process.

3. Drying process

In the secondary dehydration process, spirulina algae dehydrated at a water content in the range of 75 to 82 wt% are dried and inspected and packaged to 5-12 wt% in the drying process to produce spirulina algae products.

Dryer is one of band dryer, spray dryer, drum dryer, fluidized bed dryer, fixed bed dryer or vacuum freeze dryer Dry with a dryer.

Example 3

A vinyl house consisting of a transparent dome of PVC (Poly vinyl chloride) on top of an algae incubator (11) consisting of four concrete structures consisting of 10 m (length) x 10 m (width) x 0.2 m (depth). 10 mg / l concentration of divalent and trivalent iron salts in concentrated concentrated brine without being filtered through the reverse osmosis membrane of Example 2 in the algae culture tank 11 provided with a waterproof solar lamp 13). The solution was magnetized using neodymium permanent magnet magnetized to 12,000 gauss, and dissolved in carbon dioxide at 0.05 vol%, and caustic soda was adjusted to 10 to 30 ° C. Spirulina Platensis while irradiating 5,000 lux from the solar light (13) during the day and no sunlight at night while continuously supplying at a flow rate of 1 ㎥ / hour warmed ) And cultured into an algae settling tank (15) with a diameter of 1mΦ × 2m. The precipitated algae is returned to the end stage of the algae culture tank 11 so that the MLSS concentration of the culture medium of the algae culture tank 11 is maintained in the range of 3,000 to 3,500 mg / l by the return pump 17, and the surplus algae spirulina pratensis The production rate of was 86.5g / ㎡ · day, the algae production was 8.65kg / day was confirmed to be significantly improved than the prior art algae production rate.

As described above, the deep sea water deeper than 200m deep from the sea surface is collected, warmed and pre-filtered, and then filtered through reverse osmosis to prepare the drink, while the concentrated brine, which is not filtered, is concentrated to produce salt and brine. The production of expensive spirulina or algae using concentrated brine in the middle of the process is compared to the production method of spirulina or algae using conventional SOT medium or culture medium in which the composition of this medium is changed, or simply using deep sea water to produce spirulina or algae. It can be seen that the culture efficiency was greatly improved.

In addition, since deep sea water does not contain harmful pollutants or harmful microorganisms, it has the characteristic of producing hygienically safe spirulina or algae.

1 is a process chart for producing Spirulina algae from deep sea water

Figure 2 is a process chart of spirulina algae

<Explanation of symbols for main parts of drawing>

1: concentrated brine reservoir 2: concentrated brine transfer pump

3: magnetizer 4: carbon dioxide gas supply blower

5: CO2 supply blower 6: CO2 melting, heating and pH regulator

7: Stirrer 8: Heating jacket

9: insulation material 10: algae culture tank blower

11: algae culture 12: sun

13: lamp 14: transparent dome

15: algae settler 16: algae settler lake

17: return pump 18: salt water reservoir

19: brine transfer pump 20: surplus algae concentrate tank

21: algae sedimentation tank lake 22: concentrated algae transfer pump

FI: Flow indicator M: Motor

TI: Temperature indicator

TT: Temperature transmitter

TIC: Temperature indicating controller

pHT: pH Transmitter

pHIC: pH indicating controller

PT: Pressure transmitter

PIC: Pressure indicating controller

Claims (3)

Filtration of sand filtration, micro filtration, or ultra filtration alone or in combination of two or more that was taken from the deep sea water deeper than 200m from sea level and warmed to 20 ~ 30 ℃ After removing the suspended solids in the water (Suspended solid), and then in the reverse osmosis filtration after nanofiltration, the filtered water is sent to the beverage production process to produce concentrated concentrated brine without filtration, When the concentrated brine is supplied to the concentrated brine storage tank (1), the bivalent and trivalent iron salts are supplied, subjected to magnetization, and then dissolved in carbon dioxide, heated, and sent to a pH regulator (6) to bring the temperature to 25 to 42 ° C. adjusted to, and carbon dioxide (CO ₂₎ the volume of concentrated brine (volume) per carbon dioxide (CO ₂₎ capacity of the pH range of 9 to 11 by injecting a carbon dioxide gas, and injecting an alkaline agent to 0.03~0.08vol% range After feeding it, it is sent to the algae culture tank (11) to cultivate spirulina algae by the sun (12) light during the day, the amount of irradiation light 2000 to 6500 lux (Lux) on a cloudy day or night The concentrated brine irradiated with light from the back (13), cultured spirulina algae, and sent to the algae settling tank (15) and overflowed to the top was sent to the brine storage tank (18) together with the dewatering filtrate discharged from the dewatering process. , To the salt manufacturing process by the brine transfer pump (19), Algae precipitated by the boil to the bottom of the condensation tank (16) to the condensation (Cone) portion of the boiled together with the return pump (17) to the algae culture tank (11) for the algae culture tank (11) while culturing the surplus algae Culturing the spirulina algae, The supernatant which is supplied to the cultured spirulina algae to the surplus algae concentrating tank 20 and overflowed to the upper side of the surplus algae concentrating tank 20 is sent to the brine storage tank 18 and precipitated under the surplus algae concentrating tank 20. The concentrated algae are sent to the concentrated algae transfer pump (22) to the primary dehydrator, and the dehydrated algae is sent to the washing and secondary dehydration processes, and the dehydrated algae is sent to the drying process to dry the moisture content to 5-12 wt%, and to produce spirulina or algae products. Method for producing spirulina algae using deep sea water, characterized in that the step of producing a. The method according to claim 1, wherein the supply of divalent and trivalent iron salts is omitted, and spirulina or algae are produced using deep sea water. The method of claim 1, wherein instead of the carbon dioxide (CO ₂ ), acetic acid, citric acid, tartaric acid, palmitic acid, oxalic acid, malic acid ), Succinic acid, maleic acid, maleic acid, oxalic acid, butyric acid, fumaric acid, formic acid, picic acid, picric acid or ascorbic acid ( A method for producing spirulina or algae using deep ocean water using organic acids such as ascorbic acid or mixtures of one or more of these organic acid salts, or in combination with carbon dioxide gas.

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