KR20090060124A - Novel hydrogenases purified from thermococcus spp., genes encoding them, and methods for producing hydrogen using microorganism having the genes - Google Patents

Abstract

A hydrogenase which is isolated from a Thermococcus spp. is provided to economically and efficiently produce hydrogen by culturing a hyperthermophilic strain under the specific culture condition. A hydrogenase comprises an amino acid selected from the sequence numbers 1-112. A gene coding the hydrogenase comprises base of the sequence numbers 113-225. A method for producing a hydrogen comprises culturing Thermococcus spp. The Thermococcus spp. is Thermococcus onnurineus NA1(KCTC 10859BP). The culture is culturing in a medium of anaerobic condition supplying carbon monoxide.

Description

Novel hydrogenases purified from Thermococcus spp., Genes encoding them, and Methods for producing hydrogen using microorganisms having a new hydrogenase isolated from Tｈｅｒｍｏｃｏｃｃｕｓ ｓｐｐ. the genes}

The present invention relates to a new hydrogenase found from a new strain belonging to the genus Thermococcus , a gene encoding the same, and a method of producing hydrogen using the same.

Hydrogen energy has been spotlighted as an energy to replace fossil energy in the future because it does not emit substances that may adversely affect the environment such as carbon dioxide, NOx, and SOx while the calorific value per weight is three times higher than that of oil.

Conventionally used hydrogen production methods include electrolysis of water, thermal cracking of natural gas or naphtha, or steam reforming. However, these methods again have a problem of making high-temperature and high-pressure conditions using fossil fuels, and generate a mixed gas containing carbon monoxide, which causes a difficult problem of removing carbon monoxide from such gases.

On the other hand, the biological hydrogen production method using microorganisms does not need to make a high-temperature, high-pressure condition by inputting additional energy, and does not include carbon monoxide in the generated gas. Such biological hydrogen production methods can be broadly divided into using photosynthetic microorganisms and non-photosynthetic microorganisms (mainly anaerobic microorganisms). Examples of the former include the Republic of Korea Patent No. 10-0680624 "Hydrogen production method using the photosynthetic bacterium Rhodobacter spheroides strain excellent hydrogen production at high salt concentration" and the like.

However, high concentration culture technology of photosynthetic bacteria using light as an energy source has not yet been sufficiently developed, and conventional photosynthetic bacteria have a disadvantage of severe substrate inhibition when there is a high partial pressure substrate. In addition, they have a problem that hydrogen production performance can be continued only in the presence of light.

Therefore, attempts are continuously made to produce hydrogen using microorganisms capable of producing hydrogen using organic carbon. For example, Korean Patent No. 10-0315663 "Sysitebacterium strain Y19 and hydrogen production by this." ", Republic of Korea Patent No. 10-0315662" Rhodoshudo Monas Palustasis P4 and thereby hydrogen production "and the like.

The inventors found that the highly thermophilic mycobacteria Thermococcus A new protein of onnurineus NA1 (Accession No .: KCTC 10859BP) and a gene encoding it were filed on December 8, 2007 (Korean Patent Application No. 10-2007-0127255), the present invention is the patent application Of these proteins and genes, especially those related to hydrogen production. The inventors found that the strain produced a large amount of hydrogen even in a high temperature environment as a result of experiments related to the hydrogen generating ability of the strain, and in particular, it is possible to generate a large amount of hydrogen under culture conditions in which carbon monoxide is added. The present invention was completed.

Disclosure of Invention An object of the present invention is to provide a hydrogenase of highly thermophilic Thermococcus spp. Which can produce hydrogen even in a high temperature environment, and a gene encoding the same, and to provide a method for efficiently producing hydrogen using the same.

As a means for achieving the above object, the present invention provides a highly thermophilic Thermococcus spp. Capable of producing hydrogen under anaerobic culture conditions. Provided is a hydrogenase which a strain possesses and a gene encoding the same.

The present invention provides a method of producing hydrogen by culturing the strain and a method of producing hydrogen using the gene.

In a first aspect, the present invention provides a highly thermophilic mycobacteria Thermococcus which generates hydrogen under anaerobic conditions. onnurineus NA1 (Accession No .: KCTC 10859BP) and the hydrogenase it produces.

The strain was isolated from deep-sea hydrothermal spouts in the Park Manus region in East Manus Basin, and was deposited on October 7, 2005 to the Korea Institute of Biotechnology and Biotechnology Center (KCTC), October 2005. On day 20 he was given a deposit number of KCTC 10859BP. Characteristics and culture methods of the strains are described in Korean Patent Application No. 10-2007-0127255, which is the basic application of the present application.

More specifically, the hydrogenase provides a protein and functional equivalent thereof including any one or more amino acid sequences selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 112. “Functional equivalents” include amino acid sequence variants in which some or all of these proteins are substituted, or some of the amino acids are deleted or added. Substitutions of amino acids are preferably conservative substitutions. Examples of conservative substitutions of amino acids present in nature are as follows; Aliphatic amino acids (Gly, Ala, Pro), hydrophobic amino acids (Ile, Leu, Val), aromatic amino acids (Phe, Tyr, Trp), acidic amino acids (Asp, Glu), basic amino acids (His, Lys, Arg, Gln, Asn ) And sulfur-containing amino acids (Cys, Met). Deletion of amino acids is preferably located at portions not directly involved in the activity of the hydrogenase.

In a second aspect the present invention provides a gene encoding the hydrogenase. More specifically, the gene is a gene encoding a hydrogenase, characterized in that it comprises at least one nucleotide sequence selected from the group consisting of SEQ ID NO: 113 to SEQ ID NO: 225. The base sequences of SEQ ID NOs: 113 to 179 encode amino acids of SEQ ID NOs: 1 to 67, respectively, and the base sequences of SEQ ID NOs: 180 and 181 represent amino acids of SEQ ID NO: 68, and the base sequences of SEQ ID NOs: 182 to 225, respectively, SEQ ID NOs: The sequence encodes an amino acid from 69 to 112;

An isolated DNA segment containing the gene can be used in the form of a recombinant vector. "Vector" means a nucleic acid molecule capable of binding and transferring another nucleic acid to it. An "expression vector" includes a plasmid, cosmid or phage capable of synthesizing a protein encoded by each recombinant gene carried by the vector. Preferred vectors are vectors capable of self-replicating and expressing the nucleic acid to which they are bound.

The recombinant vector can be used to transform cells such as prokaryotes, fungi, plants, animal cells and the like to produce transformed cells capable of producing hydrogen with high efficiency. The term 'transformation' refers to the incorporation of foreign DNA or RNA into a cell, resulting in a change in the genotype of the cell.

In a third aspect the present invention is directed to Thermococcus spp. It provides a method of producing hydrogen by culturing. The Thermococcus spp. Is preferably Thermococcus onnurineus NA1 (Accession No .: KCTC 10859BP).

The culture is possible even at a high temperature of 80 ℃, can increase the efficiency when using a medium to which formate (formate) or starch (starch) is added. In addition, it can increase the efficiency if cultured by supplying medium and carbon monoxide made in anaerobic conditions.

Unlike the conventional chemical production method, the hydrogen production method according to the present invention does not require high temperature and high pressure conditions, and may generate hydrogen at room temperature and normal pressure conditions, and does not generate harmful by-products. In addition, compared with the conventional technology of producing hydrogen using microorganisms, it is possible to produce high purity hydrogen with high efficiency and has the advantage of producing hydrogen even at high temperature conditions.

Therefore, the high temperature carbon monoxide discharged from the petroleum refining process, etc. can be captured immediately without a separate cooling process and can be utilized for hydrogen generation, and can also be usefully used in terms of air purification.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the following examples are illustrative of the present invention, and the contents of the present invention are not limited by the examples.

Example  One. Thermococcus onnurineus NA1  Strain Culture and Hydrogenase Gene Analysis

(1) culture conditions

As a general culture condition, cells were cultured in yeast extract-peptone-sulfur (YPS) medium in an anaerobic environment at 80 ° C (Holden et al. 2001). Tests for physiological properties include 1.0 ml trace element mixture, 1 ml vitamin solution (Balch, WE, GE Fox, LJ Magrum, CR Woese, and RS Wolfe. 1979. Methanogens: reevaluation of a unique biological group.Microbiol. 43: 260-296.), Modified medium 1 (Sokolova, TG, C. Jeanthon, N. A Kostrikina, NA Chernyh) with addition of NaCl (30 gl ⁻¹ ) and yeast extract (0.5 gl ⁻¹ ) , AV Lebedinsky, E. Stackebrandt, and EA Bonch-Osmolovskaya. 2004.The first evidence of anaerobic CO oxidation coupled with H ₂ production by a hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent.Extremophiles 8: 317-323.) Was carried out. pH was adjusted to 8.0 using NaOH. The medium made in anaerobic conditions was placed in a 25-ml serum bottle and the remaining gas layer (15 ml) was filled with N ₂ / CO ₂ (80:20, 1 bar) or 100% CO. Formic acid salt (formate) or in the case of culture in starch (starch), pressure sterilization (autoclaving) to each 10 g L ^{before-medium} soluble starch ¹ (Sigma) ^- formate sodium (Sigma) or 5 g L ¹ Was added. All cultures for physiological testing were performed at 80 ° C. for 2 days.

(2) gene sequencing

The genome sequence of T. onnurineus NA1 was determined using whole genome shotgun sequencing and pyrosequencing. For capillary sequencing, 2- to 3-kb size insert libraries (11,028 clones), 40-kb insert libraries (1,870 clones), and 35-kb insert libraries ( 288 clones) were constructed and sequenced using the ABI 3730XL sequencer (Applied Biosystems). For fatigue sequencing, 581,990 DNA fragments were sequenced using GS-20 sequencer (454 Life Sciences). The contigs generated by both sequencers were combined and the sequencing gap was filled by clone walking and PCR sequencing. ORF and RNA genes encoding proteins were predicted through a combination of Glimmer 3.0 (University of Maryland), GSFinder, and RBSFinder and a manual ORF fitting process. After determining all ORFs, BLASTp searches (Tatusova, RL, DA Natale, IV Garkavtsev, TA Tatusova, UT) for the NCBI nr protein database, the Kyoto Encyclopedia of Genes and Genomes (KEGG), and the Clusters of Orthologous Groups of proteins (COGs) database. Shankavaram, BS Rao, B. Kiryutin, MY Galperin, ND Fedorova, and EV Koonin. 2001.The COG database: new developments in phylogenetic classification of proteins from complete genomes.Nucleic Acids Res. 29: 22-28. Protein sequence was further analyzed. tRNA-encoding sites were predicted using tRNA scan-SE (Lowe, TM, and SR Eddy. 1997. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.Nucleic Acids Res. 25: 955- 964.).

(3) protein analysis

T. onnurineus NA1 cells were suspended in 100 mM Tris-HCl buffer (pH 6.8) containing 4% SDS and 4 mM EDTA, boiled for 10 minutes, and centrifuged at 22,000 g for 20 minutes. Cell lysates were separated using 12% SDS-PAGE and 30 fractions were obtained based on molecular size. Then trypsin (Promega, USA) was used to in-gel digest them (Kim, YH, K. Cho, SH Yun, JY Kim, KH Kwon, JS Yoo, and SI). Kim. 2006. Analysis of aromatic catabolic pathways in Pseudomonas putida KT 2440 by combined proteomic approach: 2-DE / MS and cleavable ICAT analysis. Proteomics 6: 1301-1318.), Trypsin-decomposed material was dissolved in 0.5% trifluoroacetic acid solution for analysis by mass spectrometry (Thermo Finnigan LTQ IT). Peptide identification was performed using the Sequest program (Thermo Finnigan, San Jose, Calif.).

(4) Total RNA Isolation and RT-PCR Analysis

50-ml of T. onnurineus NA1 culture was added to modified medium 1 with various concentrations of yeast extract in N ₂ / CO ₂ (80:20, 1 bar) or 100% CO gas phase. The mid-exponential growth phase was raised. Cells were obtained by centrifugation at 6,000 × g for 30 minutes. The pellet was resuspended in 50 μl 50 mM Tris-HCl buffer (pH 7.5) to which 500 μl Trizol reagent (Invitrogen, Carlsbad, Calif.) Was added. The cells were lysed through freezing and thawing and samples were extracted using 200 μl of chloroform. The aqueous phase containing total RNA was further treated via ethanol precipitation and then resuspended in distilled water. The concentration and integrity of RNA was determined by 0.8% agarose gel analysis and absorbance measurements at 260 and 280 nm. Reverse transcription and PCR amplification were performed using SuperScript ^TM II Reverse Transcriptase (Invitrogen) as a method according to the product instructions. As a control, the following two sets of primers were used for the amplification of carbon monooxide dehydrogenase (CODH) and Hsp60 (chaperonine).

CODH gene (forward, 5'-GGACCATGTAGAATCGAYCCGTTY-3 '(SEQ ID NO: 226) and reverse, 5'-TTCRTTTCCGGTACAGCA-3' (SEQ ID NO: 227))

Hsp60 gene (forward, 5'-ATGGCACAGCTTAGTGGACAG-3 '(SEQ ID NO: 228) and reverse, 5'-CAAGGATTTCCTGGGCTTTCTC-3' (SEQ ID NO: 229)).

(5) Analysis result-General characteristics of gene

In the hydrothermal vent area, Thermococcus spp. In order to elucidate the reason for the successful dominant species, the genome sequence of T. onnurineus NA1 was determined by combining arbitrary wholeome shotgun sequencing and pyrosequencing. As a result, T. onnurineus NA1 has no separate exochromosomal elements and has a single circular chromosome (1,847,607 bp), resulting in a total of 1,976 coding DNA sequences. , CDSs) (Table 1, Figure 1). 1104 CDS (55.8%) can be annotated via homology and domain search, but the function of the remaining 872 CDSs could not be predicted from the primary structure. When searching for protein similarity on a genome-wide scale, 82.7% of T. onnurineus NA1 protein showed similarity to that of Thermococcales .

Table 1. General characteristics of the T. onnurineus NA1 (NA1) genome with T. kodakaraensis KOD1 (KOD1) and other Pyrococcus strains.

NA1 KOD1 P. abyssi P. horikoshii P. furiosus Gene size (bp) 1,847,607 2,088,737 1,765,118 1,738,505 1,908,256 Protein coding sites 90.1% 92.1% 91.1% 91.2% 92.5% GC content 51.0% 52.0% 44.7% 41.9% 40.8% CDSs ^a 1976 2306 1784 2064 2065 tRNAs 46 46 46 46 46 rRNAs 5S (x2), 7S, 16S, 32S 5S (x2), 7S, 16S, 32S 5S (x2), 7S, 16S, 32S 5S (x2), 7S, 16S, 32S 5S (x2), 7S, 16S, 32S

^a A protein set of strains was obtained from the RefSeq collection of NCBI.

(6) Analysis Result-Hydrogenase Cluster

The genome of T. onnurineus NA1 was found to have a high percentage of hydrogenase and related proteins (5.5%), which is a redox including CO dehydrogenase and formate dehydrogenase. It shows the preservation of the reducing power associated with the enzymes and an increase in recycling (FIG. 2). As shown in FIG. 2, Pyrococcus spp. And two membrane-bound hydrogenases, Mbh (TON_1583-1595, SEQ ID NOs: 100 to 112), Mbx (TON_0486-0498, SEQ ID NOs: 35 to 47), and two cytoplasms, reported in T. kodakaraensis KOD1. In the T. onnurineus NA1 genome, together with Sulf-I (TON_0533-0544, SEQ ID NOs: 48 to 59) and Sulf-II (TON_0051-0055, SEQ ID NOs: 1 to 5), the cytoplasmic NiFe-hydrogenase Additional hydrogenase clusters Hyg4-I (S1: TON_0261-0289, SEQ ID NOs: 6 to 34), Hyg4-II (S2: TON_1016-1031, SEQ ID NOs: 60 to 75), Hyg4-III (S3: TON_1559-1582, sequence Numbers 76 to 99). Analysis of the gene cluster of the hydrogenase with CDSs of 31 archaeal genomes revealed that the primary sequences were specific for Hyg4-I, Hyg4-II and Hyg4-III, which were hydrogenases of P. abyssi and R. rubrum . The similarity with -4 components is shown to be low (FIG. 3). Like the additional hydrogenases, the Hyg4-III cluster (TON_1559-1582, SEQ ID NOs: 76-99) is responsible for the α / β / γ subunits of the F420 hydrogenase (TON_1559-1561, SEQ ID NOs: 76-78) in the genome. Included. Α / β / γ subunits of F420 hydrogenases has had a specific primary sequence, which Methanococcus maripaludis of crude enzyme (coenzyme) F420-reducing hydrogenase ( YP_001097176) (33%) and Methanococcus It shows similarity with the coenzyme F420-reducing hydrogenase (NP_987940) (33%) of maripaludis S2 (FIGS. 3 and 5). Homologues to the F420-hydrogenase of thermococcales have not been reported. The function of this additional T. onnurineus NA1 hydrogenase is not yet clear, so further analysis is needed.

(7) Analysis result-CODH gene cluster and carboxydotrophic growth

As mentioned above, T. onnurineus NA1 was found to have specific gene clusters (Hyg4-II (S2), TON_1016-1020, SEQ ID NOs: 172 to 176), which was the hydrogenase of the Hyg4-II gene cluster (TON_1021). -1031, SEQ ID NOs: 177 to 188), together with potential transcriptional regulators (TON_1016, SEQ ID NO: 172), CODH accessory protein (CooC, TON_1019, SEQ ID NO: 175), CODH catalytic subunit ( CooS, TON_1018, SEQ ID NO: 174), and the base sequence encoding the electron transfer protein (CooF, TON_1017, SEQ ID NO: 173) (Fig. 2). Carbon monoxide (CO), heart enzymes CooS (TON_1018, SEQ ID NO: 174) of the metabolism of the microorganisms is Methanosarcina CODH (AAM06652) (60%) and Methanosarcina of acetivorans C2A mazei Significant similarities were observed with CODHs of CO-oxidizing methanogenic archaea, such as CODH (AAM29817) (59%) of Go1 (FIGS. 3 and 4). This is a single function CODH (Bonam, D., L. Lehman, GP Roberts, and PW) without the acetyl-CoA synthesis / cleavage activity of the bifunctional CODH / ACS enzyme. Ludden. 1989. Regulation of carbon monoxide dehydrogenase and hydrogenase in Rhodospirillum rubrum : effects of CO and oxygen on synthesis and activity. J. Bacteriol. 171: 3102-3107. And Wu, MQ Ren, AS Durkin, SC Daugherty, L. M Brinkac, RJ Dodson, R. Madupu, SA Sullivan, JF Kolonay, WC Nelson, LJ Tallon, KM Jones, LE Ulrich, JM Gonzalez, IB Zhulin, FT Robb , and JA Eisen. 2005. Life in hot carbon monoxide: the complete genome sequence of Carboxydothermus hydrogenoformans Z-2901. PLoS Genet . 1: e65.). Fox et al . (Fox, JD, RL Kerby, GP Roberts, and PW Ludden. 1996. Characterization of the CO-induced, CO-tolerant hydrogenase from Rhodospirillum rubrum and the gene encoding the large subunit of the enzyme. J. Bacteriol. 178: 1515-1524.), According to Rhodospirillum The rubrum 's monofunctional CODH / hydrogenase complex is involved in CO-driven proton respiration where energy is conserved by gradients of hydrogen ion concentration across the cell membrane. In this regard, to demonstrate that CODH clusters can play a similar role in energy conservation by oxidizing CO, we tested whether T. onnurineus NA1 can utilize CO. The results show that the strain actually grows better in CO gaseous environments with or without sulfur in medium 1 than in basal medium, even though the growth rate is slightly lower than in YPS medium (FIG. 6A). (FIG. 6A, b). Growth in the CO gas environment is associated with the transcription of the CooS gene, which can be induced by the presence of CO (FIG. 6C). It should be noted that the addition of sulfur reduces the transcription of the CooS gene. These results support the hypothesis that T. onnurineus NA1 generates energy from CO.

Example  2. Analysis of Gas Composition

(1) Analysis method

Hydrogen gas was measured using a gas chromatograph HP 5890 series II (Hewlett Packard) equipped with an HP-PLOT Molesieve column (Agilent) and a TCD detector. Argon was used as the gas carrier. In order to quantify hydrogen gas, a gas calibration standard (Supleco) containing 1% (w / w) of each component (CO, CO ₂ , H ₂ , CH ₄ and O ₂ ) in nitrogen was used.

(2) Hydrogen production using various materials

In order to determine if the repeated multiple hydrogenases allow T. onnurineus NA1 to produce hydrogen efficiently in various environments, hydrogen production rates were analyzed using various energy sources (Table 2). As a result, the NA1 strain showed that hydrogen can be produced using starch, CO, and formate (formate) even in the absence of sulfur. CO and formate in particular have been very good energy sources for the efficient production of hydrogen.

Table 2. Comparison of hydrogen production of T. onnurineus NA1 under various conditions

badge Hydrogen Generation (mmol / l) M + CO 30.7 M + Formate 49.7 M + starch 15.6

 M, modified medium I

Hydrogen productivity of NA1 strain in batch culture was determined by T. kodakaraensis KOD1 and Pyrococcus Similar to that obtained in continuous culture of furiosus . Hyperthermophilic archaea, despite low volumetric productivity, has a high specific production rate compared to mesophilic bacterial fermentation or hydrogen production by photosynthetic photobacteria. ) And has many advantages, such as high purity hydrogen production. The high hydrogen production rates described herein are much more likely to be improved by optimization of the culture conditions and process design or by metabolic engineering.

1 shows Thermococcales strains, T. onnurineus NA1 (NA1), T. kodakaraensis , P. furiosus , and P. abyssi . Venn diagram showing the common and specific regions of the proteome. Protein sets for these strains were obtained from the RefSeq collection of NCBI.

Figure 2 is a representative of the hydrogenase gene cluster of T. onnurineus NA1. A, B, C, D; Membrane-bound hydrogenases and cytoplasmic NiFe-hydrogenases in the cytoplasm. S1, S2, S3; T. onnurineus NA1. Hydrogenases specific for the genes were colored according to COG functional categories. TON_0051-0055 is SEQ ID NO: 1 to 5, TON_0486-0498 is SEQ ID NO: 35 to 47, TON_0533-0544 is SEQ ID NO: 48 to 59, TON_1583-1595 is SEQ ID NO: 100 to 112, and TON_0261-0289 is SEQ ID NO: 6 to 34, TON_1016-1031 shows SEQ ID NO: 60 to 75, and TON_1559-1582 shows SEQ ID NO: 76 to 99.

Figure 3 shows the distribution and conservation pattern of the hydrogenase gene cluster of 31 archaea genomes. The areas marked with blue angles (first, third, and fifth angles below) represent CDSs with low (<25%) similarity to the CDSs of 31 archaea genomes. Black areas indicate CDSs similar to P. abyssi hydrogenase 4. The archaea genes corresponding to 1 to 31 are as follows.

number gene number gene One Aeropyrum_pernix 17 Pyrococcus_abyssi 2 Pyrobaculum_aerophilum 18 Pyrococcus_furiosus 3 Sulfolobus_acidocaldarius_DSM_639 19 Pyrococcus_horikoshii 4 Sulfolobus_solfataricus 20 Thermococcus_kodakaraensis_KOD1 5 Sulfolobus_tokodaii 21 Archaeogiobus_fulgidus 6 Haloarcula_marismortui_ATCC_43049 22 Methanosarcina_barkeri_fusaro 7 Natronomonas_pharaonis 23 Methanosarcina_mazei 8 Halobacterium_sp 24 Methanosarcina_acetivorans 9 Haloquadratum_walsbyi 25 Methanospirillum_hungatei_JF-1 10 Methanococcoides_burtonii_DSM_6242 26 Methanobacterium_thermoautotrophicum 11 Picrophilus_torridus_DSM_9790 27 Methanococcus_jannaschii 12 Thermoplasma_acidophilum 28 Methanococcus_maripaludis_S2 13 Thermoplasma_volcanium 29 Methanosphaera_stadtmanae 14 Methanosaeta_thermophila_PT 30 Methanopyrus_kandleri 15 Pyrobaculum_islandicum_DSM_4184 31 Nanoarchaeum_equitans 16 Thermofilum_pendens_Hrk_5

4 and 5 compare alpha subunits of CODH and F420 hydrogenase proteins. FIG. 4 is a phylogenetic tree of CODH, and FIG. 5 is a phylogenetic tree of F420 hydrogenase alpha subunit. Homologs of each protein in the phylogenetic tree were obtained from the NCBI nr database.

6 is a growth table of T. onnurineus NA1 depending on CO. T. onnurineus NA1 was grown in medium 1 added CO (2, equilateral triangle), sulfur (3, square) or both (4, inverted triangle). As a control, those cultured in a medium without additives (1, won) and YPS medium (C) were indicated. DAPI-stained cells on the filter were counted directly with a fluorescence microscope. a , Effect of various yeast extract (YE) extract concentrations on the composition of the medium. b , Growth curve of T. onnurineus NA1 in medium 1 with other supplements added. c, Transcription analysis of the CODH gene.

<110> KOREAN OCEAN RESEARCH & DEVELOPMENT INSTITUTE <120> Novel hydrogenases purified from Thermococcus spp., Genes          encoding them, and Methods for producing hydrogen using          microorganism having the genes. <150> KR 10-2007-0127255 <151> 2007-12-08 <160> 229 <170> KopatentIn 1.71 <210> 1 <211> 107 <212> PRT <213> Thermococcus onnurineus NA1 <400> 1 Met Gly Phe Leu Ser Arg Leu Phe Gly Gly Lys Lys Glu Thr Asp Thr   1 5 10 15 Glu Glu Ile Gln Ile Val Ser Arg Lys Pro Val Gly Lys Phe His Val              20 25 30 Glu Lys Val Phe His Ile Met Gly Arg Glu Thr Leu Val Gly Thr Val          35 40 45 Glu Arg Gly Val Ile Tyr Pro Gly Tyr Lys Val Lys Gly Lys Lys Ala      50 55 60 Ala Val Ile Tyr Arg Ile Glu Lys Gly Arg Lys Ala Val Asp Phe Val  65 70 75 80 Val Asp Gly Asp Lys Ala Ala Leu Ile Leu Glu Gly Ile Thr Lys Ala                  85 90 95 Glu Glu Gly Asp Thr Leu Glu Val Tyr Gln Ser             100 105 <210> 2 <211> 415 <212> PRT <213> Thermococcus onnurineus NA1 <400> 2 Met Ile Ile Glu Leu Arg Glu Phe Thr Arg Val Glu Gly Asn Gly Lys   1 5 10 15 Ala Glu Ile Val Val Glu Asn Gly Glu Val Lys Asp Val Arg Leu Lys              20 25 30 Ile Val Glu Gly Pro Arg Phe Phe Glu Leu Leu Thr Leu Gly Arg His          35 40 45 Tyr Tyr Asp Val Pro Asp Leu Glu Ala Arg Ile Cys Ala Ile Cys Tyr      50 55 60 Leu Ser His Ser Val Ala Ser Val Leu Gly Ile Glu Lys Ala Phe Gly  65 70 75 80 Val Glu Val Ser Glu Glu Ile Gln Leu Leu Arg Glu Leu Gly Leu Ile                  85 90 95 Gly Glu Leu Leu Glu Ser His Ala Leu His Leu Tyr Leu Leu Val Ala             100 105 110 Pro Asp Val Phe Gly Tyr Pro Asp Ala Ile Arg Met Ala Thr Lys His         115 120 125 Gly Glu Leu Val Lys Glu Gly Leu Ala Leu Lys Ala Phe Gly Asn Ser     130 135 140 Ile Arg Glu Leu Ile Gly Gly Arg Glu Ile His Gly Ile Asn Val Lys 145 150 155 160 Pro Gly Gly Phe Gly Arg Tyr Pro Thr Val Glu Glu Leu Glu Asn Ile                 165 170 175 Glu Arg Glu Ser Gly Ala Leu Leu Arg Leu Ala Arg Arg Ala Val Arg             180 185 190 Leu Phe Ala Ser Leu Glu Pro Tyr Gly Glu Lys Ala Gly His Phe Val         195 200 205 Ala Thr Asp Gly Tyr Leu Trp Gly Asp Lys Leu Ile Ser Asp Glu Asp     210 215 220 Gly Ser Phe His Tyr Thr Glu Arg Ile Glu Glu Arg Ser Leu Val Tyr 225 230 235 240 Ser Phe Ala Lys Gln Ser Arg Tyr Lys Gly Glu Pro Phe Phe Val Gly                 245 250 255 Ala Leu Pro Arg Leu Leu Leu Lys Ala Glu Met Leu Thr Pro Thr Ala             260 265 270 Lys Arg Leu Phe Glu Glu His Arg Glu Lys Leu Ala Thr Gly Tyr Val         275 280 285 Ser Tyr Asn Asn Leu Ala Gln Ala Ile Glu Leu Val Tyr Ala Leu Glu     290 295 300 Arg Ala Gly Glu Ile Ala Lys Lys Leu Leu Asp Lys Gly Ile Lys Gly 305 310 315 320 Glu Asn Val Pro Val Glu Val Lys Glu Gly Glu Gly Ile Gly Tyr Val                 325 330 335 Glu Ala Pro Arg Gly Val Leu Ile His His Tyr Arg Ile Asp Ser Gly             340 345 350 Gly Lys Ile Ala Tyr Ser Asn Ile Ile Thr Pro Thr Ala Leu Asn His         355 360 365 Ala Met Met Glu Ala Ser Leu Phe Lys Glu Ala Arg Lys Leu Tyr Gly     370 375 380 Glu Thr Asp Glu Thr Val Leu Val Gln Arg Leu Glu Glu Thr Val Arg 385 390 395 400 Ala Phe Asp Pro Cys Ile Ser Cys Ser Val His Ile Val Lys Leu                 405 410 415 <210> 3 <211> 246 <212> PRT <213> Thermococcus onnurineus NA1 <400> 3 Met Met Asp Lys Leu Lys Leu Ala Val Phe Glu Leu Thr Asp Cys Gly   1 5 10 15 Gly Cys Ala Leu Asn Ile Leu Phe Leu Tyr Glu Lys Leu Phe Asp Leu              20 25 30 Leu Glu Phe Tyr Glu Ile Thr Glu Phe His Met Ala Thr Ser Leu Ser          35 40 45 Glu Gly Ser His Tyr Asp Val Ala Leu Val Thr Gly Thr Val Ser Ser      50 55 60 Gln Arg Asp Leu Ala Leu Leu Lys Glu Ala Arg Asn His Ser Asp Tyr  65 70 75 80 Leu Ile Ala Leu Gly Thr Cys Ala Thr His Gly Ser Val Gln Ala Ser                  85 90 95 Val Glu Gly Ser Ile Arg Glu Lys Leu Lys Arg Val Tyr Gly Asp Glu             100 105 110 Gly Asn Pro Met Arg Ala Leu Asp Ser Lys Pro Val Val Glu Tyr Val         115 120 125 Ala Val Asp Phe Ala Leu Pro Gly Cys Pro Tyr Asp Lys Asn Glu Val     130 135 140 Tyr Gln Val Leu Met Asp Ile Ala Lys Gly Ile Glu Pro Val Lys Lys 145 150 155 160 Asp Tyr Pro Val Cys Val Glu Cys Lys Leu Asn Glu Tyr Glu Cys Val                 165 170 175 Leu Val Lys Lys Gly Leu Pro Cys Leu Gly Pro Ile Thr Tyr Gly Gly             180 185 190 Cys Asn Ala Ala Cys Ile Arg Ser Gly Leu Gly Cys Ile Gly Cys Arg         195 200 205 Gly Pro Leu Pro Gly Glu Val Asn Pro Ala Ser Glu Tyr Glu Ile Leu     210 215 220 Lys Asp Leu Gly Tyr Asp Asp Asp Tyr Ile Leu Arg Lys Phe Lys Thr 225 230 235 240 Phe Ala Arg Trp Glu Pro                 245 <210> 4 <211> 291 <212> PRT <213> Thermococcus onnurineus NA1 <400> 4 Met Ser Glu Asn Pro His Gln Thr Tyr Asp Ala Arg Ile Leu Glu Val   1 5 10 15 Lys Asp Leu Thr Pro Arg Glu Lys Leu Phe Thr Leu Arg Phe Leu Asp              20 25 30 Pro Glu Ile Gly Glu His Phe Thr Phe Lys Pro Gly Gln Phe Val Ile          35 40 45 Val Asp Ile Arg Gly Phe Gly Glu Phe Pro Ile Ser Leu Cys Ser Ser      50 55 60 Pro Thr Arg Lys Gly Tyr Ile Gln Leu Cys Ile Arg Lys Ala Gly Arg  65 70 75 80 Met Thr Lys Phe Ile His Gln Met Lys Glu Gly Glu Val Val Gly Ile                  85 90 95 Arg Gly Pro Tyr Gly Asn Gly Phe Pro Met Glu Lys Met Glu Gly Ser             100 105 110 Asn Leu Leu Leu Val Ala Gly Gly Leu Gly Met Ala Pro Leu Arg Ser         115 120 125 Val Leu Trp Tyr Ala Ile Asp Thr Gly Lys Tyr Glu His Val Trp Leu     130 135 140 Leu Tyr Gly Thr Lys Ala Tyr Glu Asp Ile Leu Phe Arg Asp Glu Ile 145 150 155 160 Ile His Leu Leu Lys His Gly Asp Ala Val Gly Cys Ser Val Lys Leu                 165 170 175 Ala Tyr Glu Val Glu Ser Pro Ser Cys Ile Tyr Leu Glu Arg Gly Phe             180 185 190 Phe Asp Arg Val Cys Lys Gly Val Val Thr Asp Leu Phe Arg Gly Glu         195 200 205 Glu Phe Asp Val Asp Lys Ala Tyr Ala Leu Ile Cys Gly Pro Pro Val     210 215 220 Met Tyr Arg Phe Val Ile Lys Glu Leu Leu Asp Arg Lys Leu Ser Pro 225 230 235 240 Gly Arg Ile Tyr Met Thr Leu Glu Arg Arg Met Arg Cys Gly Ile Gly                 245 250 255 Lys Cys Gly His Cys Ile Val Gly Thr Ser Thr Ser Ile Lys Tyr Val             260 265 270 Cys Lys Asp Gly Pro Val Phe Thr Tyr Trp Asp Ala Leu Ser Thr Arg         275 280 285 Gly leu ile     290 <210> 5 <211> 334 <212> PRT <213> Thermococcus onnurineus NA1 <400> 5 Leu Arg Tyr Val Lys Leu Ser Ser Glu Asn Phe Ser Ser Phe Phe Glu   1 5 10 15 Ser Leu Arg Asn Trp Gly Lys Val Tyr Ala Pro Ile Lys Arg Gly Ser              20 25 30 Ile Tyr Thr Phe Gln Glu Val His Glu Leu Gly Glu Ile Glu Leu Asn          35 40 45 Tyr Thr Arg Thr Met Leu Pro Pro Lys Lys Phe Phe Val Arg Pro Arg      50 55 60 Asp Glu Ile Leu Arg Leu Lys Asn Gly Arg Trp Glu Asn Gly Thr Asp  65 70 75 80 Ala Glu Pro Ile Val Leu Phe Gly Leu His Ser Cys Asp Met His Gly                  85 90 95 Leu Lys Ile Leu Asp Lys Val Tyr Leu Asp Glu Pro Ala Asp Pro Tyr             100 105 110 Tyr Lys Ala Arg Arg Glu Lys Thr Phe Ile Val Gly Ile Ser Cys Met         115 120 125 Pro Asp Glu Tyr Cys Phe Cys Lys Ser Leu Gly Thr His Phe Ala Met     130 135 140 Asp Gly Phe Asp Leu Phe Leu His Glu Leu Pro Asp Gly Trp Leu Val 145 150 155 160 Arg Ile Gly Ser Val Arg Gly His Glu Val Val Trp Glu Asn Gly Glu                 165 170 175 Leu Phe Glu Glu Val Thr Asp Glu Asp Leu Lys His Phe Lys Glu Phe             180 185 190 Glu Glu Arg Arg Ala Asn Ala Phe Gln Lys Glu Ile Pro Gln Glu Gly         195 200 205 Leu Ala Asp Met Leu Asp Leu Ala Tyr Asn Ser Pro Val Trp Lys Glu     210 215 220 Tyr Ala Glu Ile Cys Leu Ala Cys Gly Asn Cys Asn Met Val Cys Pro 225 230 235 240 Thr Cys Arg Cys Tyr Glu Val Cys Asp Asn Trp Ile Ser Ala Tyr Asp                 245 250 255 Ala Val Arg Glu Arg Arg Tyr Asp Ser Cys Phe Met Glu Asn His Gly             260 265 270 Leu Val Ala Gly Gly His Asn Phe Arg Pro Thr Arg Leu Asp Arg Phe         275 280 285 Arg His Arg Tyr Tyr Cys Lys Ser Tyr Phe Asp Pro Ser Ser Gly Tyr     290 295 300 Asn Cys Val Gly Cys Gly Arg Cys Asp Glu Phe Cys Pro Ala Lys Ile 305 310 315 320 Glu His Val Lys Val Leu Glu Glu Val Arg Gly Ser Leu Arg                 325 330 <210> 6 <211> 139 <212> PRT <213> Thermococcus onnurineus NA1 <400> 6 Met His Glu Trp Ala Leu Ala Asp Gly Ile Val Arg Thr Ala Leu Asp   1 5 10 15 Tyr Ala Gln Arg Glu Gly Ala Lys Lys Leu Leu Ala Ile Gln Val Val              20 25 30 Leu Gly Glu Leu Gln Asp Val Asn Ala Glu Ile Val Glu Phe Ala Met          35 40 45 Lys Glu Leu Leu Lys Gly Thr Ile Gly Glu Gly Ala Glu Ile Glu Phe      50 55 60 Ile Glu Glu Glu Ala Val Phe Lys Cys Arg Asp Cys Gly His Glu Trp  65 70 75 80 Lys Leu Lys Glu Val Lys Gly Ser Phe Asp Glu Arg Ile Lys Glu Asp                  85 90 95 Ile His Phe Ile Pro Glu Val Val His Ala Phe Leu Ala Cys Pro Lys             100 105 110 Cys Gly Ser Arg Asp Phe Glu Val Val Gln Gly Arg Gly Val Tyr Ile         115 120 125 Ser Gly Ile Lys Ile Glu Lys Glu Gly Glu Ala     130 135 <210> 7 <211> 248 <212> PRT <213> Thermococcus onnurineus NA1 <400> 7 Met Ile Ala Ile Asp Pro Arg Val Lys Gly Ile Glu Ala Arg Leu Glu   1 5 10 15 Asn Val Lys Arg Ile Ile Pro Val Val Ser Gly Lys Gly Gly Val Gly              20 25 30 Lys Ser Leu Val Ser Thr Thr Leu Ala Leu Val Leu Ala Glu Lys Gly          35 40 45 Tyr Lys Val Gly Leu Leu Asp Leu Asp Phe His Gly Ala Ser Asp His      50 55 60 Val Ile Leu Gly Phe Glu Pro Glu Asp Phe Pro Glu Glu Asp Arg Gly  65 70 75 80 Val Val Pro Pro Thr Val His Gly Ile Lys Phe Met Ser Ile Val Tyr                  85 90 95 Tyr Ser Glu Asp Lys Pro Thr Pro Met Arg Gly Met Glu Ile Ser Asp             100 105 110 Ala Leu Ile Glu Leu Leu Thr Ile Thr Arg Trp Asp Glu Leu Asp Tyr         115 120 125 Leu Val Ile Asp Met Pro Pro Gly Leu Gly Asp Gln Phe Leu Asp Val     130 135 140 Leu Arg Phe Leu Lys Arg Gly Glu Phe Leu Val Val Ala Thr Pro Ser 145 150 155 160 Lys Leu Ser Leu Asn Ile Val Arg Lys Leu Leu Thr Leu Leu Lys Glu                 165 170 175 Arg Asp Tyr Lys Ile Leu Gly Ile Val Glu Asn Met Lys Leu His Ser             180 185 190 Glu Gln Arg Asn Asn Glu Lys Gly Ile Glu Ala Leu Ala Glu Glu Phe         195 200 205 Asp Val Pro Tyr Leu Ala Gly Ile Pro Leu Tyr Asp Asp Leu Glu Glu     210 215 220 Lys Ile Gly Lys Pro Glu Glu Leu Leu Arg Thr Glu Phe Ala Glu Arg 225 230 235 240 Ile Arg Glu Val Ala Gly Lys Leu                 245 <210> 8 <211> 158 <212> PRT <213> Thermococcus onnurineus NA1 <400> 8 Met Glu Leu Glu Glu Leu Phe Gln Asn Ala Lys Arg Ile Val Ile Cys   1 5 10 15 Gly Ile Gly Asn Glu Leu Arg Gly Asp Asp Ala Phe Gly Val Leu Val              20 25 30 Ala Glu Met Leu Ala Glu Leu Val Lys Ser Pro Asn Val Leu Val Ile          35 40 45 Asn Cys Gly Glu Val Pro Glu Ser Tyr Thr Gly Lys Ile Val Asp Phe      50 55 60 Lys Pro Asp Leu Ile Ile Phe Val Asp Ala Val Asp Phe Gly Gly Glu  65 70 75 80 His Gly Glu Leu Val Val Ala Asp Pro Glu Gly Thr Leu Gly Glu Ala                  85 90 95 Val Ser Thr His Ser Leu Pro Leu Lys Phe Leu Val Gln Tyr Leu Lys             100 105 110 Thr Lys Val Asn Ala Lys Phe Val Leu Ile Gly Cys Gln Pro Arg Ser         115 120 125 Leu Gly Leu Phe Glu Glu Pro Ser Gly Val Ile Arg Glu Arg Ala Glu     130 135 140 Met Leu Ala Gln Ala Ile Ala Asp Ser Leu Asn Gly Gly Glu 145 150 155 <210> 9 <211> 193 <212> PRT <213> Thermococcus onnurineus NA1 <400> 9 Met Ile Gly Ala Val Leu Ala Gly Gly Arg Gly Arg Arg Phe Gly Gly   1 5 10 15 Asn Lys Leu Leu Phe Arg Ile Asp Gly Lys Pro Leu Val Leu Tyr Ala              20 25 30 Val Glu Ala Leu Glu Ala Thr Lys Glu Ile Asp Gly Ile Val Leu Val          35 40 45 Ala Ser Pro Glu Asn Ala Glu Arg Leu Lys Lys Phe Gly Tyr Glu Val      50 55 60 Ile Val Asp Glu Leu Met Val Gly Pro Ile Gly Gly Ile Tyr Thr Ala  65 70 75 80 Leu Ser Leu Asp Asp Ala Phe Val Val Ala Gly Asp Met Pro Leu Ile                  85 90 95 Val Pro Glu Phe Val Asp Tyr Ile Ile Arg Glu Phe Arg Lys Ser Gly             100 105 110 Lys Ala Val Cys Val Pro Arg Trp Ser Asn Gly Tyr Ile Glu Pro Leu         115 120 125 His Ala Ala Tyr Ser Glu Arg Phe Arg Lys Ile Leu Glu Glu Gln Ile     130 135 140 Lys Ser Gly Asp Tyr Met Ile Arg Lys Ala Ile Glu Arg Val Asp Thr 145 150 155 160 Cys Tyr Leu Pro Ile Glu Glu Leu Pro Glu Arg Trp Arg Lys Ser Phe                 165 170 175 Phe Asn Val Asn Arg Lys Glu Asp Leu Arg Lys Leu Thr Tyr Thr Ser             180 185 190 Phe     <210> 10 <211> 146 <212> PRT <213> Thermococcus onnurineus NA1 <400> 10 Met Ser Ile Glu Ala Ala Lys Gln Ser Leu Leu Gln Arg Ile Arg Glu   1 5 10 15 Phe Tyr Gly Asp Asn Leu Leu Ser Val Val Phe Tyr Gly Ala His Leu              20 25 30 Lys Gly Gly Leu Asp Glu Ile Asp Val Leu Val Ile Ile Asp Glu Pro          35 40 45 Tyr Asp Pro Val Lys Ile Asn Arg Val Ala Asp Phe Ile Glu Asn Ile      50 55 60 Lys Glu Pro Val Glu Glu Glu Tyr Gly His Phe Ile Ala Phe Glu Leu  65 70 75 80 Tyr Thr Arg Glu Glu Ala Glu Asn Phe His Ser Ser Phe Leu Asp Val                  85 90 95 Ala Lys Ala His Glu Ile Ala Tyr Asp Arg Asp Asn Tyr Phe Gln Asn             100 105 110 Leu Leu Arg Glu Met Leu Asn Pro Lys Lys Thr Ile Glu Tyr Val Lys         115 120 125 Tyr Ile Ser Thr Ile Glu Tyr Ile Pro Val Asp Glu Glu Lys Arg Glu     130 135 140 Glu gln 145 <210> 11 <211> 521 <212> PRT <213> Thermococcus onnurineus NA1 <400> 11 Met Asp Val Val Gly Leu Thr Pro Val Ile Pro Val Ile Phe Ala Phe   1 5 10 15 Ala Leu Pro Leu Thr Ser Ile Ile Val Lys Gly Asn Arg Lys Ile Ile              20 25 30 Gln Ala Tyr Ala Leu Leu Gly Thr Gly Leu Thr Leu Ile Ser Ala Leu          35 40 45 Lys Leu Phe Glu Leu Val Tyr Ser Ser Glu Lys Pro Pro Ile Tyr Thr      50 55 60 Phe Gly Gly Trp Thr Ala Pro Ile Gly Ile Ile Tyr Glu Val Asp Arg  65 70 75 80 Thr Gly Ala Leu Ile Thr Leu Leu Thr Ala Ala Leu Met Phe Leu Ile                  85 90 95 Ala Ile Tyr Ser Tyr Arg Tyr Leu Glu His Glu Glu Gly Leu Glu Trp             100 105 110 Tyr Tyr Thr Leu Tyr Leu Gly Leu Glu Ala Gly Leu Ile Gly Val Leu         115 120 125 Leu Thr Gly Asp Ala Phe Asn Leu Phe Val Met Ile Glu Val Thr Ser     130 135 140 Ile Ala Ala Tyr Ala Leu Val Ala Phe Tyr Arg Gly Arg Lys Asp Ala 145 150 155 160 Val His Ala Ala Leu Lys Tyr Ala Phe Ile Gly Ala Ile Gly Thr Thr                 165 170 175 Met Tyr Phe Leu Ala Leu Gly Ile Leu Tyr Gly Ala Phe Gly Thr Val             180 185 190 Asn Leu Ala Asp Leu Ser Ala Lys Val His Gly Leu Ser Phe Pro Val         195 200 205 Thr Gly Ser Pro Val Gly Asn Ile Val Ile Ala Ser Gly Val Ala Leu     210 215 220 Ala Leu Ala Thr Trp Ala Phe Leu Ile Lys Ala Ala Ile Val Pro Asn 225 230 235 240 His Phe Trp Leu Pro Glu Ala His Pro Ala Ala Pro Ser Pro Ile Ser                 245 250 255 Ala Val Leu Ser Gly Leu Val Val Asn Val Gly Val Tyr Ala Leu Ile             260 265 270 Arg Phe Leu Tyr Thr Val Tyr Gly Gly Glu Leu Thr Pro Gly Leu Ala         275 280 285 Lys Ile Val Gly Thr Leu Gly Thr Val Val Ile Ala Leu Gly Ala Val     290 295 300 Ser Ala Leu Phe Gly Ala Leu Met Met Asn Ala Gln Arg Asp Val Lys 305 310 315 320 Lys Leu Ile Ala Tyr Ser Thr Ile Met His Met Gly Tyr Leu Phe Met                 325 330 335 Ala Val Gly Leu Gly Thr Gln Leu Gly Leu Gln Ala Ala Leu Phe His             340 345 350 Ile Ile Asn His Ala Ile Ala Lys Ala Leu Leu Phe Leu Ala Ala Gly         355 360 365 Met Phe Ile His Ala Ile Gly Ser Arg Asp Ile Asn Asp Met Ala Gly     370 375 380 Leu Gly Arg Gln Met Pro Met Ala Thr Leu Gly Leu Ala Ile Ala Thr 385 390 395 400 Leu Ser Leu Val Gly Ile Pro Pro Phe Asn Val Phe Phe Ser Lys Leu                 405 410 415 Leu Leu Phe Asp Ala Leu Met Glu Lys Ser Leu Ala Leu Ala Met Val             420 425 430 Ile Val Val Ser Ser Val Thr Ala Leu Val Ala Tyr Met Arg Val Phe         435 440 445 Tyr Lys Ile Trp Leu Gly Lys Pro Ser Arg Glu Val Ala Val Gly Glu     450 455 460 Ser Lys Ser Met Ala Phe Val Val Leu Leu Leu Ala Ala Ala Val Val 465 470 475 480 Val Ile Gly Leu Ala Ala Pro Val Ile Leu Gln His Tyr Ile Glu Pro                 485 490 495 Ala Ala Thr Gln Thr Met Asp Tyr Lys Leu Tyr Ile Gln Thr Ala Leu             500 505 510 Glu Tyr Ala Ser Arg Leu Lys Leu Leu         515 520 <210> 12 <211> 161 <212> PRT <213> Thermococcus onnurineus NA1 <400> 12 Met Arg Gly Val Ile Pro Thr Ala Leu Leu Ala Phe Ile Thr Tyr Ile   1 5 10 15 Ile Phe Ser Gly Ser Ser Ser Pro Tyr Asp Met Phe Thr Gly Ala Val              20 25 30 Val Ala Ile Gly Val Gly Leu Leu Met Gly Arg Tyr Val Val Gln Asn          35 40 45 Asp Ala Lys Ala Met Asn Pro Val Arg Trp Leu Trp Gly Val Ile Tyr      50 55 60 Phe Leu Trp Tyr Met Leu Val Ala Glu Thr Lys Ser His Ile Asp Val  65 70 75 80 Ile Ile Arg Ile Ile Thr Gly Asn Tyr Asn Pro Gly Ile Val Lys Val                  85 90 95 Pro Ile Gly Val Glu Thr Ser Tyr Ala Lys Thr Leu Val Ala Asn Ser             100 105 110 Ile Thr Asn Thr Pro Gly Thr Val Val Val Asp Met Asp Asp Lys Tyr         115 120 125 Phe Tyr Val Asn Trp Ile Asp Val Thr Thr Leu Asp Pro Glu Thr Ala     130 135 140 Lys Gly Glu Ile Ser Ala Asp Phe Glu Arg Phe Ala Lys Arg Ile Leu 145 150 155 160 Glu     <210> 13 <211> 130 <212> PRT <213> Thermococcus onnurineus NA1 <400> 13 Met Ser Thr Ser Glu Phe Leu Trp Ala Tyr Leu Trp Thr Val Leu Leu   1 5 10 15 Leu Thr Leu Ala Val Ser Leu Tyr Gly Ile Ile Ala Arg Pro Asn Met              20 25 30 Leu Lys Lys Ile Ile Ser Leu Thr Ile Leu Gly Asp Ala Val Asn Val          35 40 45 Met Val Val Leu Ile Gly Tyr His Leu Thr Tyr Pro Ile Ala Pro Pro      50 55 60 Ile Leu Pro Ser Leu Ser Lys Glu Ala Leu Glu Gly Phe Val Ser Ser  65 70 75 80 Ala Val Asp Pro Leu Pro Gln Ala Leu Val Ile Thr Ala Val Val Ile                  85 90 95 Gly Met Ala Val Asn Met Leu Leu Ala Val Leu Ala Ile Gln Leu Tyr             100 105 110 Arg Leu Tyr Gly Thr Leu Asp Val Arg Glu Ile Ala Lys Arg Gly Gly         115 120 125 Glu glu     130 <210> 14 <211> 257 <212> PRT <213> Thermococcus onnurineus NA1 <400> 14 Met Arg Gly Lys Ser Leu Ile Thr Ala Val Ile Ile Leu Thr Phe Ala   1 5 10 15 Ala Leu Met Thr Tyr Ala Val Ile Ser Leu Gln Val Phe Gly Glu Gly              20 25 30 Thr Gly Val Arg Pro Leu Gly Glu Phe Tyr Leu Glu Asn Ser Tyr Phe          35 40 45 Gly Asp Tyr Ser Ala Lys Ser Pro Glu Val Ile Thr Ser Ile Leu Trp      50 55 60 Asp Tyr Arg Gly Ile Asp Thr Leu Phe Glu Thr Ala Val Phe Phe Leu  65 70 75 80 Ala Ile Ile Gly Ser Leu Thr Val Phe Arg Leu Thr Lys Glu Gln Glu                  85 90 95 Lys Glu Val Lys Lys Ala Glu Ser Thr Pro Thr Glu Leu Thr Pro Ile             100 105 110 Val Lys Asp Val Thr Lys Val Ile Val Val Met Ile Leu Ala Val Ser         115 120 125 Ala Ser Ile Ala Leu His Gly His Leu Thr Pro Gly Gly Gly Phe Gln     130 135 140 Gly Gly Ser Ala Leu Ala Val Ala Pro Leu Leu Ile Ile Ala Ala Tyr 145 150 155 160 Ser Lys Tyr Thr Leu Glu Gly His Gly Leu Asp Lys Thr Arg Ala Leu                 165 170 175 Ile Leu Arg Ser Ile Gly Leu Leu Gly Ile Ala Leu Val Ala Leu Val             180 185 190 Pro Leu Leu Ser Gly Gly Phe Ile Met Gln Asn Gln Pro Ile Phe Pro         195 200 205 Ala Glu Ile Glu Ser Gln Leu Ile Gly Gly Ser Leu Ile Tyr Tyr Asn     210 215 220 Phe Phe Glu Phe Leu Ala Val Gly Val Gly Phe Thr Ala Val Phe Leu 225 230 235 240 Leu Leu Ser Ile Pro Glu Glu Lys Phe Lys Lys Ile Leu Gly Val Lys                 245 250 255 Lys     <210> 15 <211> 84 <212> PRT <213> Thermococcus onnurineus NA1 <400> 15 Val Ile Glu Val His Leu Leu Ile Leu Ser Leu Ala Leu Ile Leu Gly   1 5 10 15 Phe Ile Ala Ser Tyr Leu Ala Val Met Glu Arg Asp Leu Leu Lys Ala              20 25 30 Val Gly Phe Ser Ser Val Gln Ala Ile Ala Tyr Ala Ile Ala Phe Tyr          35 40 45 Ile Leu Met Ala Pro Asp Ile Val Leu Thr Tyr Ile Ala Ile Ala Val      50 55 60 Gly Ile Tyr Ser Ala Leu Leu Ile Phe Ala Ile Ser Lys Thr Glu Arg  65 70 75 80 Tyr Glu Val Val                 <210> 16 <211> 118 <212> PRT <213> Thermococcus onnurineus NA1 <400> 16 Met Ser Ile Leu Phe Tyr Ile Gly Ala Leu Leu Ile Val Ile Gly Ala   1 5 10 15 Leu Cys Asp Phe Phe Gly Ala Leu Gly Leu Leu Arg Phe Pro Asn Phe              20 25 30 Tyr Val Arg Leu His Ala Ala Thr Val Gly Ile Ile Gly Gly Ala Ala          35 40 45 Val Pro Leu Phe Gly Val Ala Leu Leu Ala Leu Gly Ala Asp Phe Leu      50 55 60 Pro His Arg Tyr Ala Ile Ala Gly Ala Ser Phe Ile Thr Gly Ile Ile  65 70 75 80 Val Leu Leu Ala Ser Pro Ala Gly Ser His Ala Leu Ala Tyr Ala Ala                  85 90 95 His Arg Ala Lys Leu Val Glu Trp Glu Pro Lys Val Asp His Leu Gly             100 105 110 Glu Val Arg Lys Ser Asp         115 <210> 17 <211> 94 <212> PRT <213> Thermococcus onnurineus NA1 <400> 17 Met Ser Leu Glu Ser Gln Phe Phe Leu Leu Met Lys Phe Val Ile Pro   1 5 10 15 Val Tyr Leu Leu Ala Phe Ile Ile Tyr Ala Val Arg Ala Phe Lys Gly              20 25 30 Pro Thr Ile Ala Asp Ile Ile Leu Ala Val Asp Cys Leu Ser Phe Asp          35 40 45 Val Ala Ala Phe Met Ala Ile Leu Ala Val Tyr Phe Lys Ser Val Tyr      50 55 60 Leu Val Ser Gly Ala Met Ile Leu Ala Leu Trp Gly Tyr Leu Leu Asp  65 70 75 80 Ile Tyr Ile Ala Lys His Leu Val Ser Lys Glu Val Gly Ala                  85 90 <210> 18 <211> 101 <212> PRT <213> Thermococcus onnurineus NA1 <400> 18 Met Pro Thr Lys Arg Gly Thr Trp His Leu Leu Lys Leu Val Tyr Val   1 5 10 15 Asn Pro Pro Tyr Val Ser Lys Ala Met Ala His Phe Ala Gly Asp Met              20 25 30 Ala Val Leu Glu Glu Ile Gly Thr Leu Asn Glu Lys Ser Val Glu Ile          35 40 45 Ile Lys Glu Ser Leu Gly Ala Lys Gly Asn Glu Ile Asp Leu Ser Ile      50 55 60 Gly Asp Leu Ile Leu Ile Pro Val Glu Arg Leu Pro Glu Asp Asp Arg  65 70 75 80 Lys Leu Leu Leu Lys Val Ala Gln Glu Leu Gly Ala Asp Ala Lys Leu                  85 90 95 Lys Ile Val Arg Gly             100 <210> 19 <211> 266 <212> PRT <213> Thermococcus onnurineus NA1 <400> 19 Met Ser Gly Leu Lys Ser Val Trp Val Phe His Val Asp Ser Gly Ser   1 5 10 15 Cys Asn Gly Cys Asp Ile Glu Ile Leu Asp Val Leu Thr Pro Tyr Tyr              20 25 30 Asp Ala Glu Arg Leu Gly Ile Lys Leu Val Pro Ser Pro Arg His Ala          35 40 45 Asp Ala Leu Leu Val Ser Gly Pro Leu Thr Arg Gln Thr Tyr Tyr Ala      50 55 60 Val Lys Ala Ala Tyr Glu Ala Met Pro Pro Lys Pro Arg Ile Val Val  65 70 75 80 Ala Ile Gly Thr Cys Ala Ser Ser Gly Gly Ile Phe Tyr Asn Gly Tyr                  85 90 95 Pro Ile Tyr Asn Pro Asn Pro Glu Arg Gly Ser Asp Arg Leu Arg Thr             100 105 110 Gly Gly Ile Glu Val Leu Leu Ala Glu Tyr Gly Lys Lys Pro Asp Met         115 120 125 Tyr Ile Pro Gly Cys Pro Pro Ser Pro Glu Glu Ile Leu Tyr Gly Leu     130 135 140 Ala Gln Leu Leu Gly Leu Lys Glu Lys Lys Met Lys Gly Glu Tyr Tyr 145 150 155 160 Tyr Ala Asp Glu Ile Glu Phe Val Leu Pro Glu Arg Pro Ile Glu Glu                 165 170 175 Arg Ile Tyr Leu Thr Leu Arg Glu Ser Leu Arg Arg Val Val Gly Tyr             180 185 190 Phe Asp Arg Glu Lys Val Leu Glu Asp Phe Met Ala Leu Val Glu Lys         195 200 205 Ala Gln Glu Ser Glu Asn Pro Arg Glu Arg Leu His Glu Leu Val Ile     210 215 220 Gly Tyr Phe Leu Arg Glu Lys Asp Ser Arg Val Lys Phe Ala Ile Arg 225 230 235 240 Phe Leu Glu Asn Glu Tyr Trp Arg Leu Lys Asp Ala Tyr Glu Lys Arg                 245 250 255 His Leu Ala Leu Val Lys Ala Gly Val Arg             260 265 <210> 20 <211> 205 <212> PRT <213> Thermococcus onnurineus NA1 <400> 20 Met Ala Gln Ala Ile Ser Phe Thr Asp Arg Leu Lys Phe Trp Lys Arg   1 5 10 15 Pro Glu Glu Asp Val Lys Lys Ala Pro Val Thr Thr Ser Tyr Pro Phe              20 25 30 Val Asp Ile Glu Lys Pro Pro Glu Tyr Arg Gly Ile Pro Arg Ile Asp          35 40 45 Pro His Leu Cys Ile Gly Cys Gly Ala Cys Val Arg Ala Cys Pro Pro      50 55 60 Asp Ala Leu Thr Ile Glu Trp Asp Phe Glu Asn Gly Arg Lys Arg Ile  65 70 75 80 Val Phe Asn Ala Ala Arg Cys Ile Arg Cys His Arg Cys Val Glu Val                  85 90 95 Cys Pro Thr Gly Ala Met Gln Gly Thr Thr Arg Phe Glu Ile Ala Thr             100 105 110 Pro Asn Lys Glu Asp Leu Ile Glu Val Val Asp His Lys Leu Tyr Arg         115 120 125 Cys Pro Arg Cys Gly Arg Tyr Glu Glu Phe Thr Glu Arg Gln Ile Gly     130 135 140 Lys Met Phe Gln Ile Leu Pro Glu Glu Val Ile Asp Gln His Gly Ile 145 150 155 160 Ala Glu Arg Ala Phe Leu Cys Arg Glu Cys Arg Met Glu Glu Ser Ala                 165 170 175 Lys Thr Leu Ala Val Gln Gly Pro Tyr Ala Asp Ser Leu Leu Leu Ser             180 185 190 Leu Tyr Pro Arg Gly Ser Lys Val Met Gly Glu Arg Arg         195 200 205 <210> 21 <211> 583 <212> PRT <213> Thermococcus onnurineus NA1 <400> 21 Met Glu Cys Ser Val Cys Ala Gly Gly Cys Arg Ser Ala Glu Val Glu   1 5 10 15 Asp Val Leu Glu Asp Gly His Leu Lys Glu Phe Val Glu Lys Phe Arg              20 25 30 Gly Ala Ile Phe Glu Cys Lys Lys Leu Thr Arg Asn Gln Tyr Leu Phe          35 40 45 Ile Val Asp Arg Glu Ala Leu Pro Glu Met Val Leu His Trp His Asn      50 55 60 His Ser Glu Leu Lys Glu Thr His Phe Ser Met Gly Thr Gly Thr Asp  65 70 75 80 Glu Arg Asn Ile Ala Gly Lys Phe Thr Tyr Ala Pro Val Ile Asn Val                  85 90 95 Ala Val Glu Pro Gly Asn Gly Glu Arg Asn Tyr Trp Val Ile Leu Lys             100 105 110 Ala Tyr Leu Asp Glu Asp Asn Pro Glu Phe Pro Ser Ile Ala Ala Lys         115 120 125 Leu Pro Ala Ala Leu Trp Ala Glu Arg Glu Val Tyr Asp Leu Leu Gly     130 135 140 Phe Asn Pro Lys Gly His Pro Asp Leu Arg Arg Leu Val Leu Pro Glu 145 150 155 160 Asp Trp Pro Glu Gly Val Tyr Pro Leu Arg Lys Asp His Asp Tyr Lys                 165 170 175 Ala Ser Pro Met Asp Thr Pro Lys Cys Tyr Tyr Lys Pro Gly Pro Pro             180 185 190 Asp Thr Met Thr Val Pro Ile Gly Pro Tyr His Leu Ala Leu Asp Glu         195 200 205 Pro Ala His Phe Arg Ile Phe Val Lys Gly Glu Thr Val Val Asp Val     210 215 220 Asp Tyr Arg Gly Phe Tyr Ser His Arg Gly Ile Glu Lys Ile Gly Glu 225 230 235 240 Gly Arg Leu Thr Tyr Asn Gln Val Leu Phe Ile Ala Glu Arg Ile Cys                 245 250 255 Gly Ile Cys Gly Phe Gln His Ser Thr Ser Tyr Ala Gln Ala Val Glu             260 265 270 Asn Ile Ala Gly Val Glu Ile Pro Glu Arg Ala Met Tyr Ile Arg Thr         275 280 285 Ile Met Leu Glu Ile Glu Arg Ile His Ser His Met Leu Trp Ala Gly     290 295 300 Val Ala Ala His Leu Thr Gly Phe Asp Thr Gly Phe Met His Ala Trp 305 310 315 320 Arg Val Arg Glu Pro Val Met Trp Leu Ala Glu Arg Leu Thr Gly Asn                 325 330 335 Arg Lys Thr Tyr Gly Ile Asn Ile Val Gly Gly Val Arg Arg Asp Phe             340 345 350 Leu Asp Tyr Arg Lys Glu Met Ile Met Glu Lys Ile Lys Glu Leu Arg         355 360 365 Arg Gln Val Glu Glu Phe Ile Glu Ile Ala Thr Gly Thr Ala Thr Phe     370 375 380 Val Lys Arg Ala Glu Gly Val Gly Ile Leu Pro Tyr Lys Val Ala Lys 385 390 395 400 Ala Tyr Ser Val Leu Gly Pro Asn Gly Arg Ala Ser Gly Arg Asn Ile                 405 410 415 Asp Ile Arg Arg Asp Gln Pro Phe Ala Ala Tyr Lys Asp Leu Asp Phe             420 425 430 Lys Val Pro Val Tyr Lys Glu Gly Asp Val Leu Ala Arg Phe Leu Ile         435 440 445 Arg Met Asp Glu Val Leu Glu Ser Ile Trp Ile Ile Glu Gln Ala Ile     450 455 460 Asp Gln Met Pro Gly Gly Asp Val Phe Val Pro Ile Gly Glu Leu Pro 465 470 475 480 Glu Tyr Glu Glu Ala Leu Gly Tyr Ser Glu Ala Pro Arg Gly Glu Val                 485 490 495 Ile His Tyr Val Met Thr Asp Lys Lys Asn Lys Val Tyr Arg Trp Lys             500 505 510 Val Arg Ala Pro Thr Tyr Asn Asn Leu Pro Ala Val Pro Glu Met Leu         515 520 525 Lys Gly Tyr Ser Val Ala Asp Ala Pro Leu Ile Ile Ala Ser Ile Asp     530 535 540 Pro Cys Tyr Ser Cys Thr Glu Arg Val Gln Ile Val Asp Val Glu Thr 545 550 555 560 Gly Lys Ala Gln Thr Leu Asn Glu Gln Gln Phe Asn Met Leu Ser Ile                 565 570 575 Gln Lys Gly Lys Gly Val Ala             580 <210> 22 <211> 300 <212> PRT <213> Thermococcus onnurineus NA1 <400> 22 Met Asp Tyr Val Ser Ile Ila Ala Ala Pro Ile Val Leu Phe Leu Leu   1 5 10 15 Pro Pro Phe Leu Asp Gly Ile Gly Arg Arg Ile Lys Ala Arg Ile Gln              20 25 30 Tyr Arg Arg Gly Pro Pro Ile Met Gln Thr Phe Tyr Asp Leu Glu Lys          35 40 45 Leu Leu Lys Leu Pro Ser Val Leu Pro Thr Glu Gly Pro Ile Phe Arg      50 55 60 Leu Ala Pro Tyr Ile Ala Leu Ala Ser Ala Ile Ala Gly Gly Leu Met  65 70 75 80 Leu Pro Phe Gly Ser Glu Pro Val Leu Ala Phe Gly Lys Ser Leu Ile                  85 90 95 Val Phe Phe Tyr Val Met Ala Met Val Ser Val Val Met Ile Leu Ala             100 105 110 Ala Phe Ser Val Gln Asn Ala Phe Ser His Ile Gly Gly His Arg Glu         115 120 125 Val Met Leu Ile Leu Ser Ile Glu Pro Val Leu Ala Val Val Phe Gly     130 135 140 Val Leu Ala Phe Lys Leu Gly Thr Leu Asn Val Ala Glu Met Pro Phe 145 150 155 160 Ser Ala Asn Leu Ser Leu Ser Val Ala Leu Ala Tyr Ile Leu Leu Ala                 165 170 175 Tyr Ala Val Tyr Val Glu Gly Gly Phe Val Pro Phe Asp Ile Ala Glu             180 185 190 Ala Glu Thr Glu Val Ile Gly Gly Pro Leu Thr Glu Tyr Ser Gly Arg         195 200 205 Leu Leu Gly Val Phe Lys Tyr Ala Leu Leu Val Lys Arg Val Val Leu     210 215 220 Leu Trp Leu Leu Ala Ser Met Ile Val Ile Pro Ala Met Arg Ser Leu 225 230 235 240 Gly Ile Thr Ser Ser Met Ala Leu Leu Val Ala Gln Leu Val Val Thr                 245 250 255 Phe Leu Leu Tyr Ser Leu Ala Val Ala Val Glu Ala Ala Asn Ala Arg             260 265 270 Leu Arg Ile Asp Gln Ala Val Ser Leu Asn Lys Lys Val Phe Leu Met         275 280 285 Ser Leu Ala Val Leu Ile Ile Ala Leu Val Gly Trp     290 295 300 <210> 23 <211> 659 <212> PRT <213> Thermococcus onnurineus NA1 <400> 23 Met Met Glu Ile Pro Ile Ala Leu Tyr Ser Leu Ser Ala Ile Ser Gly   1 5 10 15 Leu Ile Gly Asp Phe Lys Arg Ser Ile Lys Ile Ser Ser Val Leu Ser              20 25 30 Ala Ile Ala Ser Leu Ser Leu Leu Gly Ile Ala Ala His Ala Leu Ser          35 40 45 Arg Gly Leu Pro Val Gln Glu Ser Phe Leu Gly Ile Pro Leu Ile Ile      50 55 60 Asp Ser Leu Ser Leu Pro Phe Leu Phe Ile Ila Ala Leu Leu Ser Leu  65 70 75 80 Val Val Ser Val Tyr Ser Ile Ser Tyr Met Glu Val His Arg Asp Thr                  85 90 95 Gly Arg Pro Leu Ala Tyr Thr Ile Leu Tyr Gly Thr Phe Val Leu Ser             100 105 110 Ile Val Phe Val Ala Leu Thr Ser Asn Leu Leu Trp Phe Val Phe Phe         115 120 125 Trp Glu Leu Met Thr Leu Thr Ser Phe Ile Phe Val Ser Trp Arg Glu     130 135 140 Gln Asp Ala Gly Ile Lys Tyr Leu Leu Thr Met Gln Leu Ala Asn Thr 145 150 155 160 Val Pro Leu Phe Val Ala Leu Gly Ile Ile Tyr Ser Ala Thr Gly Ser                 165 170 175 Phe Ser Val Asp Tyr Ala Thr Leu Arg Glu Val Ala Ser Ser Leu Ser             180 185 190 Pro Val Gln Leu Lys Leu Leu Tyr Ala Met Phe Leu Val Thr Phe Leu         195 200 205 Ala Lys Ser Gly Ser Val Pro Phe Gln Phe Trp Val Pro Asp Ala Tyr     210 215 220 Glu Ala Ala Pro Ser Asn Ile Ala Ser Leu Met Ala Gly Val Met Glu 225 230 235 240 Lys Met Ala Val Tyr Gly Leu Ile Arg Leu Leu Cys Asn Ala Leu Pro                 245 250 255 Cys Ser Glu Gly Ile Gly Tyr Val Leu Val Ile Val Gly Ile Leu Thr             260 265 270 Met Thr Phe Gly Thr Leu Tyr Ala Leu Arg Glu Thr His Ala Lys Arg         275 280 285 Leu Leu Ala Tyr Ser Ser Val Gly Gln Met Gly Tyr Ile Trp Phe Ala     290 295 300 Val Gly Met Gly Met Ile Phe Leu Thr Met Gly Met Glu Ser Leu Ala 305 310 315 320 Tyr Leu Ala Phe Leu Ala Gly Val Phe His Ser Phe Asn His Thr Leu                 325 330 335 Phe Lys Gly Leu Leu Phe Leu Ile Ser Gly Asn Phe Glu Tyr Ser Ala             340 345 350 Gly Thr Ala Asp Leu Asn Glu Leu Gly Gly Leu Arg Arg Ala Met Pro         355 360 365 Tyr Ser Ser Leu Phe Thr Val Ile Gly Ala Leu Ser Leu Ala Gly Val     370 375 380 Pro Leu Phe Ser Gly Phe Leu Ser Lys Trp Met Ile Tyr Gln Ala Gly 385 390 395 400 Tyr Tyr Ser Gly Ile Gly Leu Phe Val Phe Gly Ser Val Met Ala Val                 405 410 415 Phe Met Ser Ala Val Thr Leu Ala Tyr Ser Leu Lys Leu Tyr Thr Ser             420 425 430 Ala Phe Gly Gly Glu Pro Asn Glu Arg Thr Glu Asn Ala Arg Glu Val         435 440 445 Pro Ser Gly Met Leu Leu Gly Glu Gly Ile Ile Ala Leu Thr Ser Leu     450 455 460 Ala Val Gly Ile Leu Pro Ala Ile Ala Tyr Pro Ile Leu Thr Ile Ser 465 470 475 480 Leu Asn Gly Gly Asp Val Thr Val Thr Met Gly Ser Ile Ser Thr Asp                 485 490 495 Phe Glu Tyr Phe Ser Pro Ile Ala Leu Leu Leu Ala Val Ser Phe Ile             500 505 510 Ala Val Ala Ser Tyr Phe Val Phe Arg Pro Lys Thr Thr Asn Val Lys         515 520 525 Pro Trp Asn Thr Gly Ala Leu Phe Leu Pro Glu Glu Arg Tyr Gly Ala     530 535 540 Lys Ala Arg Asp Tyr Tyr Arg Gln Tyr Phe Thr Glu Met Glu Gly Leu 545 550 555 560 Tyr Lys Leu Gly Ser Ala Ala Gly Lys Val Gly Arg Val Leu Leu Ser                 565 570 575 Ala Leu Met Ser Val Tyr Leu Val Leu Ala Arg Gly Leu Val Tyr Thr             580 585 590 Gly Arg Glu Lys Lys Arg Ser Phe Thr Leu Asp Glu Leu Arg His Arg         595 600 605 Thr Val Arg Tyr Leu Asp Glu Ala Phe Phe Ala Pro Met Met Asp Leu     610 615 620 Leu Lys Asn Ile Ala Val Leu Ala Ala Gly Ile Ser Val Ser Met Asp 625 630 635 640 Glu Leu Phe Leu Ala Ser Met Leu Thr Thr Val Ile Ile Leu Ala Leu                 645 650 655 Leu Val Leu             <210> 24 <211> 483 <212> PRT <213> Thermococcus onnurineus NA1 <400> 24 Met Asn Ala Ser Pro Phe Ile Ile Ser Phe Leu Ile Pro Leu Leu Leu   1 5 10 15 Gly Pro Leu Leu Phe Lys Leu Asp Gly Arg Lys Ala Asp Val Phe Met              20 25 30 Leu Ile Ala Val Val Ser Ser Phe Leu Ala Asn Leu Val Gly Val Leu          35 40 45 Glu Tyr Leu Lys Val Gly Gly Ala His His Ile Val Tyr Leu Glu Thr      50 55 60 Ser Ser Leu Gly Glu Val Tyr Gly Val Ile Ile Asp Pro Met Ser Val  65 70 75 80 Leu Val Gly Phe Val Val Ser Leu Ala Gly Val Leu Phe Leu Leu Tyr                  85 90 95 Ala Val Asp Tyr Met Ser Glu Arg Asn Lys Gln His Pro Val Tyr Ser             100 105 110 Asp Lys Gly Arg Phe Tyr Ala Trp Met Val Ile Phe Val Gly Ala Thr         115 120 125 Leu Ala Phe Ile Tyr Ser Ser Thr Thr Leu Gln Leu Leu Ile Phe Phe     130 135 140 Glu Ile Met Gly Leu Ala Cys Trp Gly Val Val Gly Tyr Tyr Lys Gly 145 150 155 160 Pro Lys Ala Glu Arg Ala Ala Tyr Lys Ala Leu Leu Val Pro Asn Phe                 165 170 175 Gly Ala Met Val Gly Leu Tyr Thr Thr Val Gly Ile Gly Ile Leu Lys             180 185 190 Leu His Asp Leu Ser Ile Tyr Ala Leu Gln Asn Leu Asn Asp Glu Leu         195 200 205 Lys Leu Leu Val Phe Leu Gly Val Met Val Ala Ala Phe Thr Lys Ser     210 215 220 Ala Gln Phe Pro Leu Tyr Ser Trp Leu Pro Asp Ala Met Ala Ala Pro 225 230 235 240 Thr Pro Ala Ser Ala Phe Leu His Gly Ala Ala Met Val Glu Met Gly                 245 250 255 Val Tyr Leu Leu Ala Arg Val Thr Gln Phe Met Gln Pro Ile Pro Glu             260 265 270 Thr Ala Phe Tyr Val Met Leu Val Phe Val Ser Leu Thr Leu Leu Ile         275 280 285 Ala Ile Leu Tyr Tyr Pro Leu Gln Lys Asp Ala Lys Arg Leu Leu Ala     290 295 300 Tyr Ser Thr Ile Ala Glu Ala Gly Val Met Tyr Val Gly Val Leu Tyr 305 310 315 320 Ala Val Leu Gly Ser Val Tyr Gly Leu Gln Ala Ala Met Phe Gln Leu                 325 330 335 Ala Asn His Ala Phe Val Lys Gly Leu Ala Phe Leu Thr Ala Gly Thr             340 345 350 Phe Ser Tyr Ala Phe Gly Thr Leu Asp Met Glu Lys Ile Arg Gly Leu         355 360 365 Gly Lys Leu Val Pro Val Val Gly Ala Ser Trp Phe Leu Ala Leu Leu     370 375 380 Gly Leu Ala Gly Val Pro Pro Leu Gly Leu Phe Phe Ser Lys Ala Tyr 385 390 395 400 Leu Phe Met Asn Ala Ser Ser Ile Thr Ser Trp Val Gly Trp Ile Pro                 405 410 415 Leu Phe Leu Val Leu Ala Asp Ala Thr Val Phe Leu Ala Val Ser Leu             420 425 430 Gly Trp Ile Lys Arg Met Val Phe Ser Glu Pro Leu Gln Glu Ser Ala         435 440 445 Glu Val Ser Pro Leu Met Arg Phe Val Leu Val Val Leu Ile Val Leu     450 455 460 Ser Ile Val Ala Pro Phe Leu Ser Val Lys Leu Val Thr Gln Ile Gly 465 470 475 480 Phe Met Gly             <210> 25 <211> 165 <212> PRT <213> Thermococcus onnurineus NA1 <400> 25 Met Ser Lys Lys Ile Phe Ile Asp Phe Lys Arg Cys Ile Ala Cys Lys   1 5 10 15 Ala Cys Glu Val Ala Cys Glu Met Glu His Gly Glu Ala Arg Ile Arg              20 25 30 Val Phe Glu Phe Pro Asp Leu Thr Ser Val Ala Phe Asn Cys Arg His          35 40 45 Cys Glu Lys Ala Pro Cys Met Glu Val Cys Pro Val Asn Ala Leu Ser      50 55 60 Lys Asp Asp Asp Gly Ala Val Val Leu Asp Pro Leu Lys Cys Ile Gly  65 70 75 80 Cys Leu Met Cys Gly Leu Ala Cys Pro Phe Gly Ile Pro Lys Ile Asp                  85 90 95 Glu Tyr Asn Lys Ile Met Asp Lys Cys Asp Leu Cys Ala His Arg Arg             100 105 110 Ala Glu Gly Lys Leu Pro Ala Cys Val Ser Ala Cys Pro Thr Glu Ala         115 120 125 Leu Lys Tyr Gly Asp Ile Asn Asp Val Leu Trp Ala Arg Glu Gly Lys     130 135 140 Ile Val Ala Glu Leu Lys Asp Ile Gly Asp Arg Thr Asn Val Leu Glu 145 150 155 160 Ala Tyr Leu Ile Arg                 165 <210> 26 <211> 689 <212> PRT <213> Thermococcus onnurineus NA1 <400> 26 Met Gly Met Ala Glu Lys Arg Ile Ser Val Val Cys Pro Trp Cys Ser   1 5 10 15 Val Gly Cys Arg Phe Tyr Ile Val Asn Val Asn Gly Tyr Pro Lys Lys              20 25 30 Ile Glu Phe Asp Tyr Asp His Asp Ile Arg Asn His Gly Lys Leu Cys          35 40 45 Pro Lys Gly Val Ala Ala Phe Gln His Leu Arg His Pro Asp Arg Leu      50 55 60 Lys Lys Pro Leu Lys Arg Val Gly Glu Arg Gly Glu Gly Lys Phe Lys  65 70 75 80 Glu Ile Ser Trp Glu Glu Ala Ile Lys Glu Ile Ala Gln Lys Leu Ser                  85 90 95 Glu Ile Lys Glu Lys Tyr Gly Ser Glu Ala Leu Ala Phe Leu Gly Ser             100 105 110 Glu Arg Cys Ser Ile Glu Glu Asn Tyr Val Leu Gln Lys Leu Ala Arg         115 120 125 Ala Leu Gly Thr Asn Asn Ile Glu Tyr Val Cys Arg Met Cys Gln Ser     130 135 140 Thr Ala Val Ala Gly Lys Gly Met Val Leu Gly His Pro Gly Leu Thr 145 150 155 160 Asn Pro Phe Glu Asp Ile Leu Lys Ala Lys Val Ile Val Leu Trp Gly                 165 170 175 Tyr Asn Pro Ala Ala Thr Asn Pro Val Phe Phe Gly Gln Tyr Ile Glu             180 185 190 Lys Ala Ile Leu Asp Asn Asn Ala Thr Leu Ile Val Val Asp Pro Arg         195 200 205 Lys Thr Lys Thr Ala Lys Tyr Ala Asp Ile His Leu Gln Pro Tyr Pro     210 215 220 Gly Thr Asp Leu Ala Ile Ala Leu Ala Met Leu Asn Val Ile Ile Thr 225 230 235 240 Glu Glu Leu Tyr Asp Lys Asp Phe Val Ala Glu Arg Ala Glu Gly Leu                 245 250 255 Glu Glu Leu Ala Lys Thr Val Glu Lys Tyr Thr Pro Glu Trp Ala Glu             260 265 270 Lys Val Ser Gly Val Pro Ala Glu Leu Ile Arg Lys Ala Ala Ile Thr         275 280 285 Phe Ala Thr Ala Gly Thr Ala Ala Leu Leu Thr Asn Glu Gly Val Asn     290 295 300 Gln His Ala Asn Gly Thr Arg Thr Val Met Ala Ile Thr Glu Met Met 305 310 315 320 Val Leu Cys Gly Tyr Phe Gly Lys Glu Gly Val Met Ser Gly Ala Ile                 325 330 335 Pro Gly Ala His Asn Gly Met Gly Ala Gly Leu Met Gly Ile Gly Pro             340 345 350 His Glu Leu Pro Gly Arg Phe Pro Leu His Ala Glu Glu His Lys Arg         355 360 365 Arg Ile Glu Glu Ala Trp Gly Phe Lys Ile Pro Glu Lys Pro Gly Ile     370 375 380 Thr Tyr Val Glu Met Ile Asp Ala Ile Leu Glu Gly Lys Leu Lys Ala 385 390 395 400 Leu Tyr Val Met Gly Thr Asn Pro Ala Lys Ala Leu Pro Asn Leu Lys                 405 410 415 Lys Ala Glu Glu Ala Phe Lys Asn Ile Glu Phe Leu Val Val Gln Asp             420 425 430 Ile Phe Leu Thr Glu Thr Ala Lys Tyr Ala Asp Ile Val Leu Pro Ala         435 440 445 Ala Ala Trp Phe Glu Lys Asp Gly Thr Ala Ile Ser Phe Glu Arg Arg     450 455 460 Val Gln Arg Ser Phe Lys Ala Ala Asp Ala Pro Gly Glu Ala Lys Pro 465 470 475 480 Asp Trp Glu Ile Leu Val Met Leu Ala Lys Glu Leu Gly Phe Gly Glu                 485 490 495 Tyr Phe Asn Tyr Ser Asp Ala Asp Asp Ile Leu Arg Glu Ile Asn Arg             500 505 510 Ile Ile Pro Pro Leu Ala Gly Ala Thr Pro Glu Arg Leu Lys Lys Asn         515 520 525 Leu Lys Gly Cys Met Ile Pro Cys Pro Asp Glu Asn Thr Glu Val Pro     530 535 540 Arg Leu Phe Val Gln Gly Phe Leu Thr Pro Asn Gly Lys Ala Gln Leu 545 550 555 560 Ile Pro Val Glu Tyr Lys Glu Pro Gly Glu Val Pro Asp Glu Glu Tyr                 565 570 575 Pro Phe Trp Leu Thr Asn Tyr Arg Phe Val Gly His Phe His Thr Gly             580 585 590 Thr Met Ser His Arg Ser Lys Ser Leu Ser Lys Arg Trp Pro Glu Glu         595 600 605 Tyr Ile Glu Ile Asn Glu Asn Asp Ala Lys Arg Leu Gly Ile Lys Asp     610 615 620 Gly Asp Leu Val Arg Val Glu Thr Arg Arg Ala Ala Leu Val Leu Arg 625 630 635 640 Ala Lys Val Thr Pro His Ile Arg Glu Gly Val Val Ala Ala Pro Trp                 645 650 655 His Trp Asp Phe Asn Tyr Leu Thr Thr Asp Val Leu Asp Glu Tyr Ala             660 665 670 Lys Met Pro Glu Leu Lys Thr Ala Ala Cys Arg Ile Ser Lys Val Glu         675 680 685 Gly     <210> 27 <211> 183 <212> PRT <213> Thermococcus onnurineus NA1 <400> 27 Met Asp Thr Lys Glu Arg Ile Leu Lys Ala Ala Glu Glu Leu Phe Ala   1 5 10 15 Glu Lys Gly Tyr Asp Lys Thr Thr Val Asp Glu Ile Val Glu Lys Ala              20 25 30 Arg Val Ala Lys Gly Thr Phe Tyr Asn Tyr Phe Lys Ser Lys Glu Glu          35 40 45 Leu Ile Lys Ile Val Ala Leu Gln Ser Leu Pro Tyr Ser Ala Ile Arg      50 55 60 Glu Glu Leu Glu Lys Glu His Glu Asn Leu Ala Asp Phe Leu His Ser  65 70 75 80 Ile Ala Lys Ala Tyr Ile Val Tyr Tyr Cys Asp Pro Val Leu Arg Ser                  85 90 95 Leu Phe Phe Tyr Thr Leu Ala Val Lys Gly Lys Ile Arg Glu Val Glu             100 105 110 Glu Ile His Arg Lys Phe Cys Thr Glu Ala Ile Ser Lys Gly Ala Lys         115 120 125 Lys Ile Ser Gln Leu Ala Asn Val Asp Glu Lys Thr Ala Met Val Val     130 135 140 Phe Lys Ala Phe Tyr Gly Ala Leu Phe Ser Arg Leu Val Phe Ala Glu 145 150 155 160 Tyr Ser Cys Thr Pro Asp Val Asp Tyr Val Ser Glu Leu Ile Gln Leu                 165 170 175 Ile Glu Lys Ser Leu Lys Asn             180 <210> 28 <211> 81 <212> PRT <213> Thermococcus onnurineus NA1 <400> 28 Met Cys Leu Ala Val Pro Ala Lys Val Leu Glu Ile Asn Gly Asn Val   1 5 10 15 Ala Ile Val Asp Phe Gly Gly Val Lys Arg Glu Ala Arg Leu Asp Leu              20 25 30 Leu Pro Asp Val Lys Val Gly Asp Tyr Val Ile Val His Thr Gly Phe          35 40 45 Ala Ile Glu Lys Leu Asp Glu Glu Arg Ala Lys Glu Ile Leu Glu Ala      50 55 60 Trp Asp Glu Val Phe Arg Ile Thr Arg Gly Glu Glu Leu Pro Arg Gly  65 70 75 80 Tyr     <210> 29 <211> 118 <212> PRT <213> Thermococcus onnurineus NA1 <400> 29 Met Leu Glu Lys Phe Arg Asp Arg Gly Leu Ala Gln Lys Ile Val Lys   1 5 10 15 Lys Ile His Glu Glu Ala Asn Gly Leu Glu Glu Val Arg Phe Met His              20 25 30 Val Cys Gly Thr His Glu Asp Thr Val Thr Arg Ser Gly Ile Arg Ser          35 40 45 Leu Leu Pro Glu Asn Val Lys Ile Val Ser Gly Pro Gly Cys Pro Val      50 55 60 Cys Ile Thr Pro Val Glu Asp Ile Val Lys Met Gln Glu Ile Met Arg  65 70 75 80 Gln Ala His Glu Glu Gly Glu Arg Ile Ile Leu Thr Thr Phe Gly Asp                  85 90 95 Met Tyr Lys Ile Pro Thr Pro Arg Gly Ser Phe Ala Asp Leu Arg Ala             100 105 110 Lys Ala Met Thr Leu Gly         115 <210> 30 <211> 230 <212> PRT <213> Thermococcus onnurineus NA1 <400> 30 Val Val His Phe Ser Pro Gly Phe Glu Thr Thr Thr Ala Pro Ala Ala   1 5 10 15 Gly Met Leu Asn Ser Val Val Glu Glu Gly Leu Glu Asn Phe Lys Ile              20 25 30 Tyr Ser Val His Arg Leu Thr Pro Pro Ala Val Glu Thr Leu Val Lys          35 40 45 Gln Gly Thr Lys Phe His Gly Leu Ile Asp Pro Gly His Val Ser Thr      50 55 60 Ile Ile Gly Val Lys Gly Trp Glu Tyr Ile Thr Thr Glu Tyr Gly Ile  65 70 75 80 Pro Gln Val Ile Ala Gly Phe Glu Pro Val Asp Met Leu Leu Ala Ile                  85 90 95 Leu Leu Leu Leu Lys Met Val Lys Ala Gly Glu Ala Arg Ile Ile Asn             100 105 110 Glu Tyr Thr Arg Ala Val Arg Tyr Glu Gly Asn Val Thr Ala Gln Arg         115 120 125 Leu Val Asp Lys Phe Phe Glu Val Lys Asp Ala Arg Trp Arg Ala Leu     130 135 140 Gly Val Met Pro Lys Ser Gly Leu Glu Leu Lys Lys Glu Trp Lys Glu 145 150 155 160 Leu Glu Ile Arg Thr Tyr Tyr Asp Pro Glu Val Pro Lys Leu Pro Asp                 165 170 175 Leu Glu Lys Gly Cys Leu Cys Gly Ala Ile Leu Arg Gly Leu Ala Leu             180 185 190 Pro Pro Gln Cys Pro His Phe Gly Lys Thr Cys Thr Pro Arg Gln Pro         195 200 205 Ile Gly Pro Cys Met Val Ser Tyr Glu Gly Thr Cys Ser Ile Phe Tyr     210 215 220 Lys Tyr Gly Ala Leu Phe 225 230 <210> 31 <211> 813 <212> PRT <213> Thermococcus onnurineus NA1 <400> 31 Met Gln His Leu Leu Gln Ile Trp Gly Phe Val Leu Thr Pro Lys Val   1 5 10 15 Gln Asn Ser Phe Gln Val Phe Asn Phe Trp Phe Gln Lys Pro Ile Lys              20 25 30 Thr Val Lys Thr Lys Lys Lys Ala Val Arg Gly Met Lys Ala Tyr Arg          35 40 45 Leu His Val Gln Gly Ile Val Gln Ala Val Gly Phe Arg Pro Phe Val      50 55 60 Tyr Arg Ile Ala His Glu His Gly Leu Arg Gly Tyr Val Lys Asn Leu  65 70 75 80 Gly Asp Ala Gly Val Glu Ile Val Val Glu Gly Pro Glu Lys Asp Ile                  85 90 95 Gly Ala Phe Leu Leu Asp Leu Arg Glu Lys Leu Pro Pro Leu Ala Lys             100 105 110 Ile Glu Lys Ile Lys Lys Arg Glu Ile Pro Ile Gln Gly Phe Asp Arg         115 120 125 Phe Tyr Ile Glu Lys Ser Ser Gln Gly Gly Ser Gly Gly Asp Ser Ile     130 135 140 Ile Pro Pro Asp Ile Ala Ile Cys Glu Asp Cys Leu Arg Glu Leu Phe 145 150 155 160 Asp Pro Thr Asn Lys Arg Tyr Met Tyr Pro Phe Ile Val Cys Thr Asn                 165 170 175 Cys Gly Pro Arg Phe Thr Ile Ile Glu Asp Leu Pro Tyr Asp Arg Val             180 185 190 Asn Thr Thr Met Arg Glu Phe Pro Met Cys Asp Phe Cys Glu Ser Glu         195 200 205 Tyr Lys Asp Pro Leu Asn Arg Arg Tyr His Ala Glu Pro Val Cys Cys     210 215 220 Pro Val Cys Gly Pro Ser Tyr Arg Leu Tyr Thr Asn Asp Gly Glu Glu 225 230 235 240 Leu Ile Gly Asp Pro Leu Lys Lys Ala Ala Glu Leu Ile Asp Asn Gly                 245 250 255 Tyr Ile Val Ala Ile Lys Gly Ile Gly Gly Ile His Leu Ala Cys Asp             260 265 270 Ala Thr Arg Glu Asp Val Val Ala Glu Leu Arg Lys Arg Ile His Arg         275 280 285 Pro Gln Lys Pro Phe Ala Ile Met Ala Lys Asp Val Glu Thr Ile Glu     290 295 300 Glu Phe Ala Phe Ile Ser Pro Glu Glu Leu Glu Glu Leu Lys Ser Tyr 305 310 315 320 Arg Arg Pro Ile Met Thr Leu Arg Lys Lys Glu Pro Phe Pro Leu Pro                 325 330 335 Glu Asn Leu Ala Pro Gly Leu His Thr Ile Gly Val Met Leu Pro Tyr             340 345 350 Ala Gly Thr His Tyr Ile Leu Phe His Tyr Ser Lys Thr Pro Val Tyr         355 360 365 Val Met Thr Ser Ala Asn Tyr Pro Gly Met Pro Met Val Lys Asp Asn     370 375 380 Glu Arg Ala Phe Glu Glu Leu Lys Asp Val Ala Asp Tyr Phe Leu Leu 385 390 395 400 His Asn Arg Lys Ile Leu Asn Arg Ala Asp Asp Ser Val Val Arg Phe                 405 410 415 Val Asn Gly Arg Arg Ala Val Ile Arg Arg Ser Arg Gly Phe Val Pro             420 425 430 Leu Pro Ile Glu Ile Pro Phe Asp Tyr Arg Gly Leu Ala Val Gly Ala         435 440 445 Glu Leu Met Asn Ala Phe Gly Val Thr Lys Asn Gly Lys Ile Tyr Pro     450 455 460 Ser Gln Tyr Ile Gly Asn Thr Ser Lys Val Glu Val Leu Glu Phe Met 465 470 475 480 Arg Glu Ala Val Glu His Phe Lys Arg Ile Leu Arg Val Arg Asp Phe                 485 490 495 Asp Leu Ile Val Ala Asp Leu His Pro Gly Tyr Asn Thr Thr Lys Leu             500 505 510 Ala Met Glu Met Ala Asn Glu Leu Gly Val Glu Phe Leu Gln Val Gln         515 520 525 His His Tyr Ala His Ile Ala Ser Ile Met Ala Glu His Asn Leu Asp     530 535 540 Glu Ile Val Gly Ile Ala Val Asp Gly Val Gly Tyr Gly Thr Asp Gly 545 550 555 560 His Thr Trp Gly Gly Glu Val Ile Tyr Leu Ser Tyr Glu Asp Val Glu                 565 570 575 Arg Leu Ala His Ile Asp Tyr Tyr Pro Leu Pro Gly Gly Asp Leu Ala             580 585 590 Ser Tyr Tyr Pro Leu Arg Ala Leu Met Gly Ile Leu Ser Lys Val Tyr         595 600 605 Gly Ile Glu Glu Leu Glu Glu Val Ile Glu Lys Cys Cys Pro Lys Ala     610 615 620 Ile Glu Ser Leu Arg Tyr Gly Lys Val Glu Phe Asn Val Val Leu Thr 625 630 635 640 Gln Leu Ala Lys Glu Val Asn Thr Ser Tyr Ala Ser Ser Thr Gly Arg                 645 650 655 Val Leu Asp Ala Phe Ser Val Leu Leu Asn Val Ala Tyr Arg Arg His             660 665 670 Tyr Glu Gly Glu Pro Ala Met Lys Leu Glu Ser Phe Ala Ile Arg Gly         675 680 685 Lys Asn Asp Leu Gly Phe Ser Val Pro Val Glu Gly Glu Leu Ile Lys     690 695 700 Val Glu Glu Leu Phe Gln Gln Ala Leu Glu Ile Asn Ala Ser Pro Ala 705 710 715 720 Asp Val Ala Tyr Ser Val His Leu Ala Leu Gly Arg Ala Phe Ala Glu                 725 730 735 Ile Ala Val Glu Lys Ala Lys Glu Phe Gly Val Lys Asn Val Gly Ile             740 745 750 Ser Gly Gly Val Ala Phe Asn Glu Leu Ile Val Lys Thr Val Arg Lys         755 760 765 Ile Val Glu Ala Asn Gly Leu Arg Phe Tyr Ser Thr Tyr Glu Val Pro     770 775 780 Arg Gly Asp Asn Gly Ile Asn Val Gly Gln Ala Phe Leu Gly Gly Leu 785 790 795 800 Tyr Leu Glu Gly Tyr Leu Thr Lys Glu Asp Leu Met Leu                 805 810 <210> 32 <211> 336 <212> PRT <213> Thermococcus onnurineus NA1 <400> 32 Met Lys Ile Lys Leu Glu His Gly Ala Gly Gly Glu Ile Met Glu Glu   1 5 10 15 Leu Leu Arg Asp Val Ile Leu Lys Asn Leu Ser Leu Lys Ser Ala Gly              20 25 30 Gly Ile Gly Leu Asp Ala Leu Asp Asp Gly Ala Thr Ile Pro Phe Gly          35 40 45 Asp Lys His Ile Val Phe Thr Ile Asp Gly His Thr Val Lys Pro Leu      50 55 60 Phe Phe Pro Gly Gly Asp Ile Gly Arg Leu Ala Val Ser Gly Thr Val  65 70 75 80 Asn Asp Leu Ala Val Met Gly Ala Lys Pro Leu Ala Leu Ala Asn Ser                  85 90 95 Met Ile Ile Gly Glu Gly Phe Asp Gly Glu Asp Leu Lys Lys Ile Leu             100 105 110 Arg Ser Met Asp Lys Thr Ala Ser Glu Val Pro Val Pro Ile Val Thr         115 120 125 Gly Asp Thr Lys Val Val Glu Asp Glu Ile Gly Met Phe Val Ile Thr     130 135 140 Ala Gly Ile Gly Ile Ala Glu Arg Pro Ile Ser Asp Ala Gly Ala Lys 145 150 155 160 Val Gly Asp Ala Val Leu Ile Ser Gly Thr Ile Gly Asp His Gly Ile                 165 170 175 Ala Leu Met Ser His Arg Glu Gly Ile Ser Phe Glu Thr Glu Leu Lys             180 185 190 Ser Asp Val Ala Pro Ile Trp Glu Val Val Glu Ala Val Ala Lys Ala         195 200 205 Ile Gly Trp Glu Asn Ile His Ala Met Lys Asp Pro Thr Arg Gly Gly     210 215 220 Leu Ser Asn Ala Leu Asn Glu Met Ala Arg Lys Ala Asn Val Gly Ile 225 230 235 240 Leu Ile Arg Glu Ala Asp Val Pro Ile Arg Pro Glu Val Arg Ala Ala                 245 250 255 Ser Glu Met Leu Gly Ile Ser Pro Phe Asp Val Ala Asn Glu Gly Lys             260 265 270 Val Val Met Ile Val Pro Lys Glu His Ala Glu Glu Ala Leu Glu Ala         275 280 285 Met Arg Lys Thr Glu Lys Gly Lys Asn Ala Ala Ile Gly Glu Val     290 295 300 Ile Glu Asp Tyr Arg Gly Lys Val Leu Val Glu Thr Gly Ile Gly Gly 305 310 315 320 Lys Arg Phe Leu Glu Pro Pro Ala Gly Asp Pro Val Pro Arg Val Cys                 325 330 335 <210> 33 <211> 97 <212> PRT <213> Thermococcus onnurineus NA1 <400> 33 Met Ile Ile Ala Lys Pro Cys Val Thr Met Lys Gly Ile Val Ile Ser   1 5 10 15 Gly Tyr Ser Trp Asp Arg Lys Val Glu Val Asp Leu Thr Arg Thr Ala              20 25 30 Gln Cys Leu Lys Glu Lys Gly Tyr Val Ile Lys Lys Ile Leu Pro Gly          35 40 45 Met Met Leu Ile Leu Glu Met Glu Gly Tyr Glu Val Ser Val Tyr Pro      50 55 60 Ser Gly Lys Ile Ile Ile Lys Leu Leu Glu Asp Ser Lys Lys Gly Glu  65 70 75 80 Glu Met Ala Arg Ile Ile Tyr Asp Cys Ala Gly Ile Leu Glu Val Val                  85 90 95 Ser     <210> 34 <211> 399 <212> PRT <213> Thermococcus onnurineus NA1 <400> 34 Met Arg Ile Pro Glu Asp Val Arg Lys Asp Ile Pro Leu Thr Asn Glu   1 5 10 15 Val Ile Tyr Phe Asp Asn Thr Ala Thr Ser Leu Thr Pro Lys Pro Val              20 25 30 Val Glu Ala Met Asp Glu Tyr Tyr Leu Lys Tyr Arg Ala Asn Val His          35 40 45 Arg Gly Val His Arg Leu Ser Gln Met Ala Thr His Lys Tyr Glu Glu      50 55 60 Ser Arg Lys Ile Val Ala Asp Phe Ile Gly Ala Lys Phe Glu Glu Ile  65 70 75 80 Val Phe Thr Lys Asn Thr Ser Glu Ser Leu Asn Leu Val Ala Leu Gly                  85 90 95 Leu Gly His Ile Phe Lys Arg Gly Asp Lys Ile Val Thr Thr Pro Tyr             100 105 110 Glu His His Ser Asp Leu Leu Pro Trp Gln Arg Leu Ala Thr Lys Leu         115 120 125 Gly Leu Lys Leu Glu Phe Ile Glu Gly Asp Asp Glu Gly Asn Leu Asp     130 135 140 Leu Ser Asp Ala Glu Lys Lys Ile Lys Gly Ala Lys Leu Val Ala Val 145 150 155 160 Gln His Val Ser Asn Ala Leu Gly Val Ile His Glu Val Glu Glu Leu                 165 170 175 Gly Lys Ile Ala Lys Asp Glu Gly Ala Ile Phe Val Val Asp Ala Ala             180 185 190 Gln Ser Ala Gly His Met Glu Val Asn Val Lys Lys Leu His Ala Asp         195 200 205 Phe Leu Ala Phe Ser Gly His Lys Gly Pro Met Gly Pro Thr Gly Ile     210 215 220 Gly Val Leu Tyr Ile Arg Glu Glu Phe Phe Asp Thr Phe Glu Pro Pro 225 230 235 240 Leu Ile Gly Gly Gly Thr Ile Glu Asp Val Ser Leu Asp Gly Tyr Lys                 245 250 255 Leu Thr Glu Pro Pro Glu Arg Phe Glu Ala Gly Thr Pro Asn Ile Gly             260 265 270 Gly Ala Ile Gly Leu Ala Ala Gly Ile Arg Tyr Ile Glu Arg Ile Gly         275 280 285 Leu Gly Arg Ile Glu Arg Gln Glu His Lys Leu Val Lys Arg Thr Thr     290 295 300 Glu Gly Leu Asp Glu Leu Glu Val Pro Trp Tyr Gly Pro Arg Asn Leu 305 310 315 320 Lys Lys His Ala Gly Val Val Ser Phe Asn Val Pro Gly Leu His Pro                 325 330 335 His Asp Val Ala Ala Ile Leu Asp Asp His Ser Ile Met Val Arg Ser             340 345 350 Gly His His Cys Ala Leu Pro Val Met Lys Lys Leu Gly Ile Asn Gly         355 360 365 Thr Val Arg Ala Ser Phe His Val Tyr Asn Ser Leu Glu Glu Val Glu     370 375 380 Thr Phe Leu Gly Val Met Glu Glu Leu Val Lys Gly Leu Lys Thr 385 390 395 <210> 35 <211> 232 <212> PRT <213> Thermococcus onnurineus NA1 <400> 35 Met Glu Val Asp Phe Lys Val Ala Pro Glu Glu Lys Val Arg Lys Lys   1 5 10 15 Pro Ser Phe Ile Lys Pro Trp Met Gly Leu Lys Tyr Leu Phe Lys Lys              20 25 30 Pro Val Thr Ile Lys Ile Pro Tyr Glu Arg Val Gln Ile Ala Lys Asp          35 40 45 Tyr Arg Gly Phe His Thr Leu Asp Trp Lys Lys Cys Val Gly Cys Asn      50 55 60 Phe Cys Gly Gln Ile Cys Pro Ala Arg Ala Ile Glu Met Thr Trp Ile  65 70 75 80 Glu Val Asp Gly Lys Met Glu Lys Arg Pro His Pro Lys Ile Asp Tyr                  85 90 95 Gly Arg Cys Thr Phe Cys Glu Phe Cys Val Asp Val Cys Pro Pro Gly             100 105 110 Ala Leu Gly Phe Ile Glu Asn Tyr Ile Leu Thr Thr Glu Trp Lys Asp         115 120 125 Glu Glu Leu Glu Leu Phe Asp Trp Val Pro Ile His Pro Asp Lys Phe     130 135 140 Arg Glu Ile Asn Glu Lys Phe Pro Asp Tyr Arg Phe Pro Val Glu Lys 145 150 155 160 Ile Glu Phe Asn Lys Glu Thr Lys Glu Val Thr Tyr Tyr Leu Arg Asp                 165 170 175 Gly Glu Val Met Lys Phe Lys Ile Leu Gly Tyr Gly Ile Arg Pro Pro             180 185 190 Lys Pro Pro Thr Lys Pro Ala Gln Lys Ala Ala Ala Lys Ala Ala Glu         195 200 205 Lys Asn Asp Thr Lys Pro Val Glu Lys Pro Thr Glu Lys Lys Glu Ala     210 215 220 Gly Lys Ile Glu Glu Lys Lys Glu 225 230 <210> 36 <211> 391 <212> PRT <213> Thermococcus onnurineus NA1 <400> 36 Met Val Ser Gln Asn Glu Leu Ile Arg Glu Ala Arg Glu Asn Gly Met   1 5 10 15 Asp Leu Leu Pro Ile Asp Lys Asp Thr Tyr Glu Leu Phe Phe Gly Pro              20 25 30 Gln His Met Ala Thr Glu Asn Phe Ser Ile Ile Leu Lys Met Asp Gly          35 40 45 His Arg Val Val Lys Ala Ile Ala Asn Pro Gly Phe Leu His Arg Gly      50 55 60 Phe Glu Lys Leu Ala Glu Tyr Arg Pro Trp His Thr Asn Ile Ala Leu  65 70 75 80 Leu Leu Arg Ile Cys Val Pro Glu Pro Asp Val Pro Glu Ala Ile Tyr                  85 90 95 Ser Met Ala Val Asp Glu Ile Ile Gly Trp Glu Val Pro Glu Arg Ala             100 105 110 Gln Trp Ile Arg Thr Thr Val Leu Glu Met Ala Arg Val Ser Ala Tyr         115 120 125 Leu Phe Trp Ile Met Gly Leu Ser Phe Lys Leu Gly Val Tyr Thr Ala     130 135 140 Gly Gln Trp Ala Ala Ala Tyr Arg Glu Arg Leu Met Ala Leu Phe Glu 145 150 155 160 Gln Leu Thr Gly Ala Arg Val Tyr His Ile Tyr Thr Ile Pro Gly Gly                 165 170 175 Val Arg Arg Asp Ile Pro Gly Asp Lys Trp Leu Arg Gln Leu Lys Asp             180 185 190 Thr Val Glu Tyr Ile Arg Ser Lys Leu Ser Asp Phe Asp Asn Leu Val         195 200 205 Phe Glu Asn Tyr Val Ala His Arg Arg Leu Glu Gly Ile Gly Val Met     210 215 220 Asp Lys Lys Phe Ala Leu Ala Glu Gly Val Thr Gly Pro Asn Leu Arg 225 230 235 240 Ala Thr Gly Val Pro Tyr Asp Val Arg Arg Ala Asp Pro Tyr Leu Leu                 245 250 255 Tyr Pro Glu Leu Asp Phe Glu Val Pro Val Leu Lys Glu Gly Asp Ala             260 265 270 Leu Ala Arg Ala Leu Ile Arg Arg Phe Glu Leu Glu Gln Asp Leu Tyr         275 280 285 Ile Leu Asp Gln Leu Leu Glu Met Gly Pro Pro Ser Gly Pro Tyr Lys     290 295 300 Val Glu Asp Pro Lys Leu Lys Asn Leu Pro Arg Phe Lys Val Pro Ala 305 310 315 320 Gly Asp Ala Phe Ala His Val Glu Ser Thr Lys Gly Asp Phe Gly Ala                 325 330 335 Tyr Val Val Ser Asp Gly Lys His Lys Pro Tyr Arg Val Gln Ile Arg             340 345 350 Gly Pro Ser Ile Ala His Gly Val Arg Val Leu Glu Gln Leu Leu Val         355 360 365 Gly Ala Arg Ile Ala Asp Val Pro Val Ile Leu Met Ser Leu Asp Asn     370 375 380 Cys Pro Pro Asp Ile Asp Arg 385 390 <210> 37 <211> 177 <212> PRT <213> Thermococcus onnurineus NA1 <400> 37 Met Gly Glu Val Lys Trp Glu Arg Glu Gln Met Leu Val Asp Lys Ile   1 5 10 15 Leu Glu Lys Ala Pro Tyr Ala Glu Gly Lys Val Arg Arg Glu Arg Arg              20 25 30 Ile Glu Phe Ser Ile Pro Ala Asp Arg Ile Arg Asp Phe Leu Met Leu          35 40 45 Leu Arg Asp Asn Asp Phe Glu Leu Met Leu Gln Ile Thr Thr Val Asp      50 55 60 Trp Pro Asn Asp Gly Glu Leu Glu Leu Ile Tyr Gln Met Trp Ser Val  65 70 75 80 Thr His Arg Thr His Ala Met Val Arg Thr Arg Ile Pro Arg Asp Leu                  85 90 95 Asp Lys Ala Arg Met Pro Thr Val Lys Asp Ile Tyr Pro Val Ala Glu             100 105 110 Thr Tyr Glu Arg Asp Ala His Asp Phe Phe Gly Val Tyr Phe Glu Gly         115 120 125 Asn Glu Lys Met Glu Met Pro Trp Ile Leu Asp Asp Thr Glu Gln Gly     130 135 140 Leu Phe Pro His Arg Lys Asp Phe Asp Met Leu Thr Tyr Val Lys Lys 145 150 155 160 Lys Tyr Lys Leu Leu Asp Arg Phe Asp Glu Asp Lys Asp Asn Tyr Val                 165 170 175 Ile     <210> 38 <211> 190 <212> PRT <213> Thermococcus onnurineus NA1 <400> 38 Met Val Asp Trp Arg Leu Phe Glu Pro Leu Phe Asn Tyr Ala Arg Lys   1 5 10 15 Lys Ser Leu Trp Ile Val Ser Phe Cys Thr Gly Cys Gly Gly Ile Glu              20 25 30 Met Pro Pro Leu Met Thr Ser Arg Tyr Asp Leu Glu Arg Phe Gly Met          35 40 45 Ile Pro Asp Pro Ser Pro Arg Gln Tyr Asp Leu Phe Leu Ile Thr Gly      50 55 60 Tyr Val Thr Pro Lys Thr Leu Lys Arg Ile Ile Ile Thr Tyr Glu Met  65 70 75 80 Ala Pro Asp Pro Lys Tyr Val Leu Ala His Gly Ser Cys Pro Leu Asn                  85 90 95 Gly Gly Ile Tyr Trp Asp Ala Tyr Asn Ala Ile Lys His Leu Asp Lys             100 105 110 Tyr Ile Pro Val Asp Val Val Ile Ala Gly Cys Met Pro Arg Pro Glu         115 120 125 Ala Val Met Asp Gly Ile Gln Lys Ile Met Glu Met Ile Glu Asn Gly     130 135 140 Thr Ala Asp Gly Trp Lys Arg Tyr Lys Glu Asn Tyr Glu Trp Tyr Lys 145 150 155 160 Lys Asn Gln Asp Glu Leu Phe Gly Glu Gly Trp Arg Glu Arg Glu Ala                 165 170 175 Arg Arg Trp Ile Pro Trp Leu Val Asp Lys Lys Lys Glu Glu             180 185 190 <210> 39 <211> 309 <212> PRT <213> Thermococcus onnurineus NA1 <400> 39 Met Leu Glu Val Ala Leu Lys Ala Leu Phe Leu Leu Ile Tyr Ala Thr   1 5 10 15 Phe Val Gly Phe Met Phe Met Gly Ile Ile Arg Lys Val Ser Ala Arg              20 25 30 Ile His Arg Arg Val Gly Pro Pro Ile Tyr Gln Pro Ile Leu Asp Thr          35 40 45 Ile Lys Phe Phe Gly Lys Lys Glu Asn Ile Thr His Gly Leu Ile Tyr      50 55 60 Asp Phe Gly Ile Ile Tyr Ala Leu Gly Ala Thr Val Leu Ala Leu Phe  65 70 75 80 Phe Ile Pro Leu Gly Ser Val Ser Ile Leu Arg Ala Tyr Gly Asp Leu                  85 90 95 Ile Leu Ile Thr Phe Leu Leu Glu Ile Pro Met Leu Gly Ile Met Phe             100 105 110 Ala Ala Met Ser Ser Gly Asn Pro Tyr Ala Gly Ile Gly Ala Gln Arg         115 120 125 Ala Leu Leu Thr Met Leu Ala Ile Gln Val Pro Leu Gly Phe Ala Ile     130 135 140 Ile Ala Leu Ala Glu Phe Tyr Gly Thr Phe Ser Thr Tyr Glu Ile Val 145 150 155 160 Met Ala Gln Gln Ile Ser Gly Trp Ser Ile Ile His Leu Pro Leu Leu                 165 170 175 Leu Ala Ala Ile Ala Tyr Asp Ile Val Leu Gln Ala Met Phe Gly Lys             180 185 190 Glu Pro Phe Asp Ile Met Ile Ala Pro Gly Glu Ile Ser Leu Gly Pro         195 200 205 Met Val Glu Phe Gly Gly Lys His Met Gly Ile Leu Gln Ile Gln His     210 215 220 Ala Ile Ala Leu Phe Ala Glu Thr Leu Phe Phe Ser Asn Ile Phe Leu 225 230 235 240 Gly Gly Ala Val Ile Thr Ala Phe Ala Ser Pro Ile Leu Asn Thr Ile                 245 250 255 Ala Ser Leu Ala Ile Leu Leu Val Lys Gln Ile Ala Val Leu Leu Ile             260 265 270 Ala Val Phe Val Ser Thr Ile Phe Pro Arg Phe Thr Ile Asp Gln Ala         275 280 285 Ala Lys Phe Tyr Trp Lys Trp Pro Thr Ile Ile Ala Ala Leu Gly Ala     290 295 300 Ile Leu Ala Ser Leu 305 <210> 40 <211> 620 <212> PRT <213> Thermococcus onnurineus NA1 <400> 40 Met Ile Asn Glu Leu Ile Val Ile Ile Phe Ala Pro Leu Ile Ala Gly   1 5 10 15 Val Ile Ala Trp Ala Leu Asp Ile Lys Val Ile Arg Glu Leu Leu Gly              20 25 30 Val Ala Gly Ala Ala Leu Pro Leu Ala Tyr Leu Ile Lys Leu Tyr Pro          35 40 45 Thr Val Asp Ala Glu Gly Met Ile Gln His Thr Leu Asn Phe Ala Gly      50 55 60 Phe Thr Leu Glu Phe Thr Leu Thr His Met Ser Trp Ile Phe Ala Met  65 70 75 80 Ile Ala Gly Val Val Gly Leu Ala Ala Val Leu Gly Met Val Ser Thr                  85 90 95 Ser Lys Asn Gly Tyr Glu Trp Leu Phe Ala Leu Met Ser Leu Thr Gly             100 105 110 Val Tyr Gly Ile Phe Ile Ala Tyr Asp Leu Leu Gly Phe Phe Ile Phe         115 120 125 Trp Glu Ile Met Thr Phe Gly Ser Phe Met Met Val Leu Lys Tyr Asn     130 135 140 Arg Glu Ala Ser Leu Lys Tyr Phe Val Leu Ser Val Ile Gly Ala Tyr 145 150 155 160 Ala Met Leu Ile Ala Ile Gly Ile Ile Tyr Ala Lys Val Gly Ser Phe                 165 170 175 Ser Phe Ile Glu Val Tyr Arg Ala Phe Ser Gln Asp Ala Ala Leu Ala             180 185 190 Ala Val Gly Ser Ser Thr Ile Phe Ser Lys Gly Glu Ile Ala Leu Ile         195 200 205 Phe Gly Leu Phe Leu Val Ala Phe Gly Val Lys Ala Gly Met Phe Pro     210 215 220 Leu His Val Trp Ala Pro Asp Ala Tyr Ser Glu Thr Asn Gln Ser Tyr 225 230 235 240 Thr Ala Met Phe Ser Gly Val Leu Ser Lys Thr Gly Val Tyr Gly Phe                 245 250 255 Ile Leu Ile Tyr Met Leu Ile Gly Tyr Arg Leu Leu Tyr Glu Phe Gly             260 265 270 Thr Ile Arg Thr Val Pro Lys Phe Gly Tyr Ile Ile Ala Phe Leu Gly         275 280 285 Gly Leu Thr Ile Ile Val Gly Gly Ile Leu Ala Ala Leu Gln Glu Asp     290 295 300 Ile Arg Lys Leu Phe Ala Tyr Ser Ser Ile Ser Gln Ile Gly Tyr Ile 305 310 315 320 Leu Val Gly Ile Gly Val Gly Thr Ala Leu Ser Ile Glu Ala Ala Ile                 325 330 335 Tyr His Ala Ile Ser His Ala Leu Phe Lys Gly Leu Phe Phe Leu Ile             340 345 350 Val Ala Thr Ile Ile Tyr Arg Thr Gly Lys Thr Glu Phe Lys Asp Met         355 360 365 Gly Gly Leu Ala Glu Lys Met Pro Val Thr Phe Ala Met Ala Phe Val     370 375 380 Ala Ile Leu Ser Leu Ala Gly Ile Pro Pro Met Val Gly Phe Ala Ser 385 390 395 400 Lys Trp Leu Ile Phe Glu Ser Val Ile Ser Gln Asn Met Pro Ile Leu                 405 410 415 Gly Ala Met Val Phe Phe Gly Ser Ala Val Gly Phe Val Tyr Leu Ile             420 425 430 Arg Phe Thr Tyr Ala Val Trp Phe Gly Gln Arg Pro Thr Asp Leu Asp         435 440 445 Asp Thr Lys Asp Ala Pro Leu Pro Leu Ala Ile Gly Met Gly Ile Leu     450 455 460 Ala Val Leu Asn Val Val Phe Gly Val Ala Pro Gly Leu Val Ala Gln 465 470 475 480 Glu Leu Asn Lys Ile Phe Gly Arg Glu Val Ile Gly Gly Thr Ile Trp                 485 490 495 Glu Leu Asn Leu Gly Phe Gly Lys Tyr Asn Gly Leu Leu Leu Thr Val             500 505 510 Trp Phe Val Val Gly Met Leu Ile Ala Ala Ile Val Tyr Phe Met Gly         515 520 525 Ala Gly Val Arg Lys Val Pro Val Thr Asp Thr Tyr Gln Ser Gly Asn     530 535 540 Pro Val Thr Met Glu Tyr Asn Leu Thr Ile Arg Arg Asn Phe Phe Leu 545 550 555 560 Pro Leu Lys Glu Ala Leu Ser Phe Trp Leu Arg Met Ser Phe Asp Arg                 565 570 575 Phe Tyr Lys Asp Val Gly Lys Thr Val Glu Asp Phe Ala Glu Thr Met             580 585 590 Arg Asn Tyr Val Tyr Asn Gly Asn Val Gln Ser Tyr Ala Trp Tyr Leu         595 600 605 Ala Ile Val Leu Leu Ile Leu Ala Met Trp Gly Val     610 615 620 <210> 41 <211> 495 <212> PRT <213> Thermococcus onnurineus NA1 <400> 41 Met Asn Gly Gln Tyr Ala Ala Leu Leu Ile Ala Leu Pro Leu Ile Ser   1 5 10 15 Ala Phe Phe Val Pro Val Phe Lys Gly Met Gly Lys Asn Ala Val Arg              20 25 30 Tyr Phe Leu Ile Leu Val Thr Ala Leu Gln Thr Gly Ile Ala Ala Trp          35 40 45 Val Phe Lys Glu Val Tyr Thr Thr Gly Gln Pro Ile Ile Val Ile Ala      50 55 60 Gly Gly Trp Lys Pro Pro Val Gly Ile Asn Leu Tyr Leu Gly His Phe  65 70 75 80 Ala Ala Leu Phe Val Leu Ile Ile Ala Val Val Ser Leu Phe Thr Ser                  85 90 95 Ile Phe Ser Ile Ser Ala Val Lys Thr Glu Pro Ile Asp Lys Tyr Ala             100 105 110 Met Leu Phe Leu Leu Leu Met Leu Gly Ala Thr Gly Met Ile Ala Thr         115 120 125 Gly Asp Ile Phe Asn Leu Phe Val Phe Met Glu Ile Thr Ala Ile Thr     130 135 140 Ala Tyr Ala Leu Thr Ala Tyr Asn Lys Thr Gly Glu Ser Ala Glu Ala 145 150 155 160 Ser Met Lys Tyr Ile Ile Leu Gly Gly Ile Gly Ser Ser Phe Phe Leu                 165 170 175 Ile Gly Val Ala Leu Ile Tyr Gly Ser Leu Gly Thr Leu Asn Met Ala             180 185 190 Gln Ile Ala Gln Leu Ala Ala Thr Met Asp Pro Thr Val Ala Gln Val         195 200 205 Gly Leu Ala Leu Ile Ile Leu Gly Leu Ala Val Glu Ala Glu Leu Phe     210 215 220 Pro Leu Asn Ala Trp Ala Pro Asp Ala Tyr Gln Ala Ala Pro His Pro 225 230 235 240 Ile Thr Val Met Phe Ser Ala Phe Val Val Lys Ala Ala Leu Tyr Ala                 245 250 255 Met Ala Arg Ile Ile Tyr Leu Met Gln Val Val Glu Ser Trp Gly Ser             260 265 270 Val Leu Arg Leu Leu Ile Val Met Ala Ala Leu Thr Val Val Val Ala         275 280 285 Glu Leu Ala Ala Leu Arg Gln Lys Asn Val Lys Arg Met Ile Ala Tyr     290 295 300 Ser Ser Ile Ala Gln Ile Gly Phe Ile Ala Leu Ala Phe Ala Leu Gly 305 310 315 320 Thr Gln Ala Gly Val Asp Ala Gly Val Phe His Met Ile Asn His Ala                 325 330 335 Ile Val Lys Val Leu Leu Phe Leu Ala Val Gly Tyr Val Ala Ile Thr             340 345 350 Leu Gly Gly Ala Glu Leu Glu Lys Phe Gln Gly Leu Gly Arg Arg Met         355 360 365 Pro Leu Thr Ala Phe Ala Ile Ser Val Gly Ala Val Ala Thr Ile Gly     370 375 380 Ile Pro Leu Phe Asn Val Phe Trp Ser Lys Ile Arg Ile Ile Leu Ala 385 390 395 400 Thr Leu Asn Val Gly Tyr Thr Trp Ala Ala Val Leu Val Leu Ser Ala                 405 410 415 Ser Val Val Glu Ala Val Tyr Tyr Phe Arg Leu Leu His Ala Ile Trp             420 425 430 Phe Glu Gly Glu Gly Glu Arg Ile Ser Glu Ser Met Val Ile Gly Leu         435 440 445 Met Met Phe Phe Leu Ala Ala Leu Val Val Val Ile Gly Val Tyr Pro     450 455 460 Asp Tyr Val Trp Glu Leu Ala Arg Lys Ala Gly Glu Asp Ile Phe Asn 465 470 475 480 Val Ala Gln Tyr Val Lys Asn Val Pro Leu Met Gly Val Gly Ala                 485 490 495 <210> 42 <211> 114 <212> PRT <213> Thermococcus onnurineus NA1 <400> 42 Met Ile Ser Val Tyr Tyr Phe Gly Ala Ile Ala Leu Ile Leu Val Gly   1 5 10 15 Leu Tyr Ala Ile Leu Val Lys Lys Asn Leu Leu Lys Ile Leu Ile Gly              20 25 30 Leu Ser Ile Met Glu Thr Gly Val Asn Leu Leu Leu Ile Ser Ile Gly          35 40 45 Tyr Val Ser Gly Lys Ser Ala Pro Ile Leu Ser Glu Gly Ile Gly Pro      50 55 60 Ser Gln Ala Val Asp Pro Ile Pro Gln Ala Leu Val Leu Thr Ala Ile  65 70 75 80 Val Ile Gly Val Ala Thr Thr Ala Met Ala Leu Ser Val Ala Ile Leu                  85 90 95 Ile Tyr Glu Lys Tyr Gly Thr Leu Asn Val Glu Glu Ile Arg Arg Leu             100 105 110 Arg gly         <210> 43 <211> 236 <212> PRT <213> Thermococcus onnurineus NA1 <400> 43 Met Val Val Lys Arg Ala Leu Ala Ile Ile Thr Leu Leu Ile Ily Gly   1 5 10 15 Tyr Trp Leu Ala Gln Gly Leu Ala Gly Val Pro Phe Gly Glu Asp Lys              20 25 30 Met Leu Val Gly Gln Tyr Tyr Leu Asp Asn Val Lys Gln Gln Thr Gly          35 40 45 Ser Val Asn Ala Val Thr Ala Val Val Val Asn Tyr Arg Gly Phe Asp      50 55 60 Thr Leu Gly Glu Val Thr Val Leu Phe Ile Ala Ser Thr Gly Val Gly  65 70 75 80 Ala Leu Leu Trp Arg Arg Lys Lys Glu Arg Thr Ala Lys Thr Glu Gly                  85 90 95 Ser Ile Val Leu Thr Thr Gly Thr Arg Leu Leu Phe Pro Phe Val Met             100 105 110 Leu Phe Gly Ala Tyr Ile Phe Ile His Gly His Leu Thr Pro Gly Gly         115 120 125 Gly Phe Pro Gly Gly Ala Thr Ile Ala Thr Ala Phe Leu Leu Leu Tyr     130 135 140 Leu Ala Phe Ile Thr Tyr Glu Ile Pro His Arg Gly Phe Glu Val Thr 145 150 155 160 Glu Gly Leu Ala Gly Met Ser Tyr Val Ala Val Gly Leu Val Gly Leu                 165 170 175 Ala Ile Gly Gly Tyr Phe Leu Phe Asp Trp Ile Trp Gln Thr Trp Gln             180 185 190 Phe Gly Trp Asp Asn Val Gly Arg Leu Leu Ser Gly Gly Phe Ile Pro         195 200 205 Ile Ile Tyr Thr Ile Ile Gly Ile Lys Val Gly Thr Glu Leu Ser Gly     210 215 220 Ile Ile Asp Asn Met Leu Lys Glu Glu Val Ser Glu 225 230 235 <210> 44 <211> 94 <212> PRT <213> Thermococcus onnurineus NA1 <400> 44 Met Asn Leu Ala Tyr Tyr Ile Pro His Leu Ile Val Ala Ile Met Val   1 5 10 15 Val Ser Ala Ile Leu Ala Val Glu Trp Lys Asp Ile Leu Ala Ala Ala              20 25 30 Val Gly Met Ala Ala Val Ser Leu Phe Ala Ser Leu Leu Phe Phe Phe          35 40 45 Leu Gln Ala Pro Asp Val Ala Met Thr Glu Ala Ala Ile Gly Ala Ala      50 55 60 Leu Ser Gly Ala Val Phe Ile Phe Ala Ile Lys Arg Thr Gln Arg Phe  65 70 75 80 Glu Thr Glu Glu Glu Glu Thr Pro Gly Trp Trp Val Arg Trp                  85 90 <210> 45 <211> 136 <212> PRT <213> Thermococcus onnurineus NA1 <400> 45 Met Asn Ala Leu Thr Val Ile Gly Glu Val Leu Val Leu Leu Gly Thr   1 5 10 15 Phe Phe Tyr Phe Leu Ser Ala Leu Gly Leu Ile Arg Met Pro Asp Val              20 25 30 Tyr Asn Arg Met Gln Thr Ser Thr Lys Ser Ala Thr Leu Gly Ser Leu          35 40 45 Gly Val Ile Val Gly Val Gly Ile Trp Ala Leu Gly Thr Asp Phe Gly      50 55 60 Ser Ala Ala Trp Leu Thr Lys Thr Ile Val Ile Ala Val Phe Leu Leu  65 70 75 80 Leu Thr Asn Pro Ile Ser Ala His Ala Leu Ile Arg Ala Ala Tyr Lys                  85 90 95 Ser Gly Ile Pro Leu Trp Glu Gly Ser Ile Val Asp Lys Tyr Ser Glu             100 105 110 His Leu Glu Ala Lys Val Lys Ala Glu Glu Thr Pro Glu Glu Thr Asp         115 120 125 Glu Thr Gly Glu Gly Gly Glu Glu     130 135 <210> 46 <211> 84 <212> PRT <213> Thermococcus onnurineus NA1 <400> 46 Met Ile Gly Ile Asn Val Tyr Leu Ala Leu Ile Ala Ile Ala Thr Leu   1 5 10 15 Leu Ser Met Tyr Arg Ile Phe Arg Gly Pro Thr Thr Val Asp Arg Ile              20 25 30 Val Ala Val Asp Ile Met Thr Thr Ile Thr Thr Gly Leu Met Val Leu          35 40 45 Phe Ala Leu Tyr Tyr Gln Arg Met Ile Phe Leu Asp Val Ala Leu Val      50 55 60 Tyr Ala Ile Leu Ala Phe Gly Gly Val Met Ala Phe Ala Arg Tyr Met  65 70 75 80 Glu Gly Gly Leu                 <210> 47 <211> 168 <212> PRT <213> Thermococcus onnurineus NA1 <400> 47 Met Gly Glu Ala Ser Lys Ile Ser Arg Tyr Leu Tyr Thr Val Ile Val   1 5 10 15 Leu Phe Leu Ile Trp Leu Phe Leu Thr Ala Ser Leu Asp Pro Gln Glu              20 25 30 Leu Gly Phe Gly Leu Leu Leu Ser Leu Ile Val Gly Ala Phe Thr Tyr          35 40 45 Glu Ile Phe Thr Thr Asn Gly Leu Ala Asn Leu His Pro Lys Arg Ile      50 55 60 Ala Tyr Ala Ile Ala Tyr Ile Pro Tyr Phe Leu Trp Ala Met Ile Met  65 70 75 80 Ala Asn Leu Asp Val Ala Tyr Arg Val Leu His Pro Lys Arg Pro Ile                  85 90 95 Asn Pro Gly Ile Val Glu Cys Arg Thr Val Leu Lys Ser Asp Val Gly             100 105 110 Lys Leu Ser Leu Ala Asn Ser Ile Thr Leu Thr Pro Gly Thr Ile Thr         115 120 125 Leu Asp Val Asp Gly Asp Arg Tyr Phe Ile His Trp Ile Asp Val Lys     130 135 140 Asp Asp Ser Val Glu Gly Ala Ser Lys Ser Ile Thr Glu Pro Phe Glu 145 150 155 160 Lys Phe Leu Lys Val Ile Phe Gly                 165 <210> 48 <211> 149 <212> PRT <213> Thermococcus onnurineus NA1 <400> 48 Met Glu Thr Leu Ile Leu Ala Leu Gly Asn Glu Leu Met Lys Asp Asp   1 5 10 15 Gly Val Gly Leu Lys Val Gly Arg Leu Leu Ala Glu Lys Gly Tyr Asn              20 25 30 Val Leu Glu Val Gly Thr Asp Ile Phe Met Leu Gln Ser His Tyr Ser          35 40 45 Gly Glu Glu Arg Leu Ile Ile Ile Asp Ala Ile Leu Ser Glu Lys Phe      50 55 60 Lys Pro Gly Glu Ile Ile His Val Ser Gly Gly Glu Val Phe Glu Lys  65 70 75 80 Leu Lys Ala Glu Ile Arg Ser Ala His Phe Met Gly Ala Ile Asp Gly                  85 90 95 Leu Lys Leu Leu Met Ala Leu Asp Glu Arg Leu Ala Asn Val Glu Ile             100 105 110 His Phe Ile Gly Ile Val Ala Lys Glu Ile Asn Leu Gly Met Glu Leu         115 120 125 Thr Glu Glu Val Arg Glu Ala Leu Pro Lys Ala Val Glu Leu Val Glu     130 135 140 Glu Leu Val Lys Lys 145 <210> 49 <211> 429 <212> PRT <213> Thermococcus onnurineus NA1 <400> 49 Met Lys Asn Leu Tyr Leu Pro Ile Thr Val Asp His Ile Ala Arg Val   1 5 10 15 Glu Gly Lys Gly Gly Val Glu Ile Val Val Gly Asp Asp Gly Val Lys              20 25 30 Glu Val Lys Leu Asn Ile Ile Glu Gly Pro Arg Phe Phe Glu Ala Ile          35 40 45 Thr Ile Gly Lys Lys Leu Asp Glu Ala Leu Ala Val Tyr Pro Arg Ile      50 55 60 Cys Ser Phe Cys Ser Ala Ala His Lys Leu Thr Ala Val Glu Ala Ala  65 70 75 80 Glu Lys Ala Val Gly Phe Glu Val Arg Glu Glu Ile Gln Ala Leu Arg                  85 90 95 Glu Val Leu Tyr Ile Gly Asp Met Ile Glu Ser His Ala Leu His Leu             100 105 110 Tyr Leu Leu Val Leu Pro Asp Tyr Met Gly Tyr Ser Asn Pro Leu Lys         115 120 125 Met Leu Asp Lys Tyr Lys Lys Glu Ile Asn Ile Ala Leu Asp Leu Lys     130 135 140 Asn Leu Gly Ser Trp Met Met Asp Glu Leu Gly Ala Arg Ala Ile His 145 150 155 160 Gln Glu Asn Val Val Met Gly Gly Phe Gly Lys Leu Pro Asp Lys Thr                 165 170 175 Thr Leu Glu Lys Met Lys Lys Arg Leu Gln Glu Ala Leu Pro Leu Ala             180 185 190 Glu Tyr Thr Phe Glu Leu Phe Ser Lys Leu Glu Gln Tyr Glu Glu Val         195 200 205 Glu Gly Pro Ile Ile His Met Ala Val Arg Pro Arg Gly Asp Val Tyr     210 215 220 Gly Ile Tyr Gly Asp Ala Ile Ser Val Ser Asp Gly Phe Glu Phe Pro 225 230 235 240 Ser Glu Gly Tyr Lys Lys His Met Val Glu Arg Val Val Glu His Ser                 245 250 255 Phe Ala Lys His Ser Phe Tyr Asn Gly Glu Lys Pro Phe Met Thr Gly             260 265 270 Ala Ile Ser Arg Val Val Asn His Ala Asp Lys Leu Tyr Gly Arg Ala         275 280 285 Lys Glu Leu Tyr Glu Ser His Lys Asp Leu Leu Arg Pro Thr Asn Pro     290 295 300 Phe Ala Asn Asn Leu Ala Gln Ala Leu Glu Leu Val Tyr Phe Met Glu 305 310 315 320 Arg Gly Ile Asp Leu Ile Asp Glu Ala Leu Ala Lys Trp Pro Ile Arg                 325 330 335 Pro Arg Asp Glu Val Asp Val Lys Asp Gly Phe Gly Val Ser Thr Thr             340 345 350 Glu Ala Pro Arg Gly Ile Leu Val Tyr Ala Leu Glu Val Lys Asp Gly         355 360 365 Arg Val Ala Tyr Ala Asp Ile Ile Thr Pro Thr Ala Phe Asn Leu Ala     370 375 380 Met Met Glu Val His Val Arg Met Met Ala Glu Lys His Tyr Asn Asp 385 390 395 400 Asp Pro Glu Arg Leu Lys Leu Leu Ala Glu Met Val Val Arg Ala Tyr                 405 410 415 Asp Pro Cys Ile Ser Cys Ser Val His Val Ala Arg Leu             420 425 <210> 50 <211> 265 <212> PRT <213> Thermococcus onnurineus NA1 <400> 50 Met Glu Gly Lys Val Arg Ile Gly Phe Tyr Ala Leu Thr Ser Cys Tyr   1 5 10 15 Gly Cys Gln Leu Arg Phe Ala Met Met Asp Glu Ile Leu Gln Leu Leu              20 25 30 Pro Asn Ala Glu Ile Val Cys Trp Tyr Met Leu Asp Arg Asp Ser Ser          35 40 45 Glu Asp Glu Pro Val Asp Ile Ala Phe Ile Glu Gly Ser Val Ser Thr      50 55 60 Glu Glu Glu Val Glu Leu Val Lys Lys Ile Arg Glu Asn Ala Lys Ile  65 70 75 80 Val Val Ala Val Gly Ala Cys Ala Thr Gln Gly Gly Val Gln Ser Trp                  85 90 95 Glu Lys Asp Lys Ser Leu Glu Glu Leu Trp Lys Ala Val Tyr Gly Asp             100 105 110 Gly Lys Val Lys Phe Glu Pro Lys Met Ala Glu Pro Leu Glu Asn Tyr         115 120 125 Ile Lys Val Asp Tyr Arg Ile Tyr Gly Cys Pro Pro Glu Lys Lys Asp     130 135 140 Phe Ile Tyr Ala Ile Gly Thr Phe Leu Val Gly Ser Trp Pro Glu Asp 145 150 155 160 Ile Asp Tyr Pro Val Cys Leu Glu Cys Arg Leu Lys Gly Asn Thr Cys                 165 170 175 Ile Leu Ile Glu Lys Gly Glu Pro Cys Leu Gly Pro Ile Thr Arg Ala             180 185 190 Gly Cys Asp Ala Arg Cys Pro Ser Tyr Gly Ile Ala Cys Ile Gly Cys         195 200 205 Arg Gly Ala Ile Gly Tyr Asp Val Ala Trp Phe Asp Ser Leu Ala Arg     210 215 220 Thr Phe Lys Glu Lys Gly Leu Thr Lys Glu Glu Ile Leu Glu Arg Met 225 230 235 240 Lys Ile Phe Asn Ala His Asn Pro Lys Leu Glu Glu Met Val Asp Lys                 245 250 255 Val Phe Gln Phe Gln Gly Val Lys Glu             260 265 <210> 51 <211> 291 <212> PRT <213> Thermococcus onnurineus NA1 <400> 51 Met Asn Glu Ala His Val Cys Met Cys His Asp Asn Pro Tyr Ala Leu   1 5 10 15 Asp Arg Val Lys Val Leu Arg Val Tyr Arg Leu Thr Glu Thr Glu Lys              20 25 30 Leu Phe Leu Phe Arg Phe Glu Asp Gln Glu Ile Ala Glu Asn Trp Thr          35 40 45 Phe Lys Pro Gly Gln Phe Val Gln Leu Thr Ile Pro Gly Val Gly Glu      50 55 60 Val Pro Ile Ser Ile Cys Ser Ser Pro Met Lys Arg Gly Phe Phe Glu  65 70 75 80 Leu Cys Ile Arg Lys Ala Gly Arg Val Thr Thr Val Val Val His Lys Leu                  85 90 95 Lys Pro Gly Asp Thr Val Leu Val Arg Gly Pro Tyr Gly Asn Gly Phe             100 105 110 Pro Val Asp Glu Trp Glu Gly Met Asp Leu Leu Leu Ile Ala Ala Gly         115 120 125 Leu Gly Thr Ala Pro Leu Arg Ser Val Phe Leu Tyr Ala Met Asp Asn     130 135 140 Arg Trp Lys Tyr Gly Asn Ile Thr Phe Ile Asn Thr Ala Arg Tyr Gly 145 150 155 160 Lys Asp Leu Leu Phe Tyr Lys Glu Leu Glu Ala Met Lys Asp Leu Ala                 165 170 175 Glu Ala Glu Asn Val Gln Ile Ile Gln Ser Val Thr Arg Asp Pro Asp             180 185 190 Trp Pro Gly Arg His Gly Arg Pro Gln Lys Phe Ile Val Glu Ala Asn         195 200 205 Thr Asn Pro Lys Asn Thr Ala Ile Ala Ile Cys Gly Pro Pro Arg Met     210 215 220 Tyr Lys Ala Val Phe Glu Ala Leu Ile Asn Tyr Gly Tyr Arg Pro Glu 225 230 235 240 Asn Ile Tyr Val Thr Leu Glu Arg Lys Met Lys Cys Gly Ile Gly Lys                 245 250 255 Cys Gly His Cys Asn Val Gly Thr Ser Thr Ser Trp Lys Tyr Val Cys             260 265 270 Arg Asp Gly Pro Val Phe Thr Tyr Phe Asp Ile Val Ser Thr Pro Gly         275 280 285 Leu Leu Asp     290 <210> 52 <211> 367 <212> PRT <213> Thermococcus onnurineus NA1 <400> 52 Met Arg Tyr Val Lys Leu Pro Lys Glu Asn Thr Tyr Glu Phe Leu Glu   1 5 10 15 Arg Leu Lys Asn Leu Gly Lys Leu Tyr Ala Pro Val Lys Ile Ser Asp              20 25 30 Gln Phe Tyr Asp Phe Arg Glu Ile Asp Asp Val Arg Lys Ile Glu Phe          35 40 45 Asn Tyr Thr Arg Thr Leu Met Pro Pro Lys Lys Phe Phe Phe Ala Pro      50 55 60 Arg Glu Lys Met Phe Glu Phe Ser Ile Ser Lys Ala Glu Tyr Arg Glu  65 70 75 80 Val Ile Pro Glu Val Glu Pro Phe Val Leu Phe Gly Leu His Ala Cys                  85 90 95 Asp Ile Tyr Gly Leu Lys Ile Leu Asp Ser Val Tyr Leu Asp Glu Tyr             100 105 110 Pro Asp Lys Tyr Tyr Lys Val Arg Arg Glu Lys Gly Ile Ile Ily Gly         115 120 125 Ile Ser Cys Met Pro Asp Glu Tyr Cys Phe Cys Asn Leu Leu Arg Thr     130 135 140 Asp Phe Glu His Asp Gly Phe Asp Leu Phe Phe His Glu Leu Pro Asp 145 150 155 160 Gly Trp Leu Ile Arg Ile Gly Thr Pro Thr Gly His Arg Ile Val Asp                 165 170 175 Lys Asn Ile Lys Leu Phe Thr Glu Val Ala Gln Glu Asp Ile Cys Asn             180 185 190 Phe Arg Glu Phe Glu Arg Lys Arg Ala Gln Ala Phe Arg Tyr His Glu         195 200 205 Glu Trp Asp Asn Ile His Tyr Leu Leu Glu Leu Glu Met Glu His Pro     210 215 220 Leu Trp Glu Lys Glu Ala Glu Lys Cys Phe Ala Cys Gly Asn Cys Ser 225 230 235 240 Thr Val Cys Pro Thr Cys Arg Cys Tyr Glu Val Gln Asp Ile Val Asn                 245 250 255 Leu Asp Gly Asp Thr Gly Tyr Arg Glu Arg Arg Trp Asp Ser Cys Lys             260 265 270 Phe Arg Ser His Gly Leu Val Ala Gly Gly His Asn Phe Arg Pro Thr         275 280 285 Lys Lys Asp Arg Phe Ile Asn Arg Tyr Leu Cys Lys Met Ser Phe His     290 295 300 Trp Thr Leu Gly Ile Asn Phe Cys Val Gly Cys Gly Arg Cys Thr Ala 305 310 315 320 Phe Cys Pro Ala Gly Ile Asp Phe Val Lys Asn Leu Arg Ile Ile Ala                 325 330 335 Gly Leu Glu Asp Ala Ser Cys Pro Ser Lys Leu Ser Glu Glu Ile Pro             340 345 350 Lys Lys Gly Phe Ala Tyr Ala Asn Asn Ile Arg Gly Glu Asp Ile         355 360 365 <210> 53 <211> 329 <212> PRT <213> Thermococcus onnurineus NA1 <400> 53 Met Ala Gln Asn Asn Ser Leu Val Leu Tyr Asp Val His Glu Thr Val   1 5 10 15 Asp Val Cys Ser Asn Val Gly Cys Val Lys Thr Lys Ala Thr Pro Ser              20 25 30 Arg Leu Leu Phe Ala Gly Phe Met Ala Gly Ala Tyr Ile Ala Phe Gly          35 40 45 Phe Ile Phe Ala Ile Val Ala Ser Ala Ser Phe His Pro Lys Leu Gly      50 55 60 Thr Phe Pro Asn Leu Ser Leu Phe Lys Leu Leu Leu Gly Ala Val Phe  65 70 75 80 Pro Val Gly Leu Ile Ala Val Leu Leu Gly Gly Ala Asp Leu Trp Thr                  85 90 95 Gly Asn Ala His Ile Val Thr Leu Ser Lys Met Thr Gly Arg Ala Ser             100 105 110 Val Lys Asp Val Leu Tyr Asn Trp Ile Gly Ser Tyr Thr Gly Asn Phe         115 120 125 Val Gly Ser Val Phe Leu Ala Phe Leu Ala Val Tyr Gly Thr Gly Leu     130 135 140 Met Ala Gly Gly Leu Phe Lys Asp Val Leu Ile Gly Ile Gly Asn Tyr 145 150 155 160 Lys Val Ala Leu Thr Pro Trp Lys Ala Leu Trp Leu Gly Ile Gly Cys                 165 170 175 Asn Trp Leu Val Asn Val Ala Ile Trp Leu Tyr Ile Arg Ala Lys Asp             180 185 190 Thr Ala Gly Lys Val Ile Val Thr Trp Phe Pro Ile Phe Ala Phe Val         195 200 205 Ala Ile Gly Phe Glu His Ser Ile Ala Asn Met Trp Ala Ile Ser Ala     210 215 220 Ser Ile Phe Ala Ser Asp Gly Ala Ile Ser Trp Val Gln Phe Phe His 225 230 235 240 Asn Ile Ile Pro Val Thr Ile Gly Asn Ala Ile Gly Gly Phe Leu Phe                 245 250 255 Val Gly Phe Tyr His Trp Tyr Leu Ala Asp Gly Arg Asn Ala Ile Lys             260 265 270 Glu Leu Ile Asp Phe Val Glu Val Leu Ala Leu Phe Val Phe Ile Met         275 280 285 Val Leu Ile Pro Ala Gly Ile Ala Tyr Ala Leu Ser Gly Leu Gly Asn     290 295 300 Ile Ala Thr Trp Leu Val Pro Leu Ile Ile Ser Val Tyr Gly Val Val 305 310 315 320 Met Thr Tyr Leu Val Arg Arg Ala Leu                 325 <210> 54 <211> 680 <212> PRT <213> Thermococcus onnurineus NA1 <400> 54 Met Glu Glu Phe Lys Ile Gly Leu Cys Pro Tyr Cys Gly Met Gly Cys   1 5 10 15 Arg Phe Tyr Ile Lys Thr Leu Asn Gly Gln Pro Ile Gly Ile Glu Pro              20 25 30 Tyr Pro Gly Gly Val Asn Glu Gly Lys Leu Cys Pro Lys Gly Val Ala          35 40 45 Ala Val Asp Phe Leu Arg His Lys Asp Arg Leu Lys Lys Pro Leu Lys      50 55 60 Arg Thr Glu Asn Gly Phe Val Glu Ile Ser Trp Glu Gln Ala Ile Lys  65 70 75 80 Glu Ile Ala Glu Lys Leu Leu Glu Ile Arg Glu Lys Tyr Gly Pro Asp                  85 90 95 Thr Leu Gly Phe Phe Ser Ser Ala Arg Cys Ser Asn Glu Glu Asn Tyr             100 105 110 Leu Leu Gln Lys Ile Ala Arg Leu Leu Gly Thr Asn Asn Val Asp His         115 120 125 Cys Ala Arg Leu Cys His Ala Ser Thr Val Val Gly Leu Ala Gln Thr     130 135 140 Val Gly Ala Ala Ala Gln Ser Gly Ser Tyr Thr Asp Ile Pro Lys Ala 145 150 155 160 Lys Val Leu Leu Ile Trp Gly Tyr Asn Pro Ser Glu Thr His Pro Val                 165 170 175 Leu Met Arg Tyr Ile Leu Arg Ala Arg Asp Asn Gly Ala Lys Ile Ile             180 185 190 Val Val Asp Pro Arg Lys Thr Arg Thr Val Trp Phe Ala Asp Met His         195 200 205 Leu Gln Leu Lys Pro Gly Thr Asp Ile Val Leu Ala Asn Ala Met Met     210 215 220 His Val Ile Ile Glu Glu Arg Leu Tyr Asp Arg Glu Phe Ile Met Asn 225 230 235 240 Arg Thr Lys Gly Phe Glu Lys Leu Ile Ala Ala Val Gln Lys Tyr Thr                 245 250 255 Pro Glu Tyr Ala Glu Glu Ile Thr Gly Val Pro Ala Lys Leu Ile Arg             260 265 270 Glu Ala Ala Ile Thr Phe Ala Thr Ala Gly Arg Gly Ile Val Met Trp         275 280 285 Ala Met Gly Leu Thr Gln His Val Thr Gly Ala Ala Asn Val Lys Ala     290 295 300 Leu Ala Asp Leu Ala Leu Ile Cys Gly Tyr Val Gly Arg Glu Gly Thr 305 310 315 320 Gly Leu Phe Pro Met Arg Gly Gln Asn Asn Val Gln Gly Ala Cys Asp                 325 330 335 Met Ala Ala Leu Pro Asn Val Phe Pro Gly Tyr Gln Lys Val Thr Asp             340 345 350 Asp Glu Lys Arg Lys His Val Ala Glu Ile Trp Gly Val Glu Asp Leu         355 360 365 Pro Ser Lys Pro Gly Leu Thr Ile Pro Glu Met Ile Asp Ala Ala Ala     370 375 380 Lys Gly Glu Leu Lys Ala Leu Tyr Ile Met Gly Glu Asn Pro Val Met 385 390 395 400 Ser Asp Pro Asn Thr Lys His Val Ile Glu Ala Leu Lys Asn Leu Glu                 405 410 415 Leu Leu Val Val Gln Asp Ile Phe Leu Thr Glu Thr Ala Glu Leu Ala             420 425 430 His Tyr Val Leu Pro Ala Ala Ala Tyr Ala Glu Lys Glu Gly Ser Phe         435 440 445 Thr Ala Ser Glu Arg Arg Val Gln Trp Asn Phe Lys Ala Ile Glu Pro     450 455 460 Pro Gly Glu Ala Lys Pro Asp Trp Glu Ile Leu Thr Met Leu Gly Lys 465 470 475 480 Ala Leu Gly Leu Pro Lys Phe Asp Tyr Ser Asp Val Glu Asp Ile Thr                 485 490 495 Arg Glu Ile Thr Leu Val Ala Pro Gln Tyr Arg Gly Ile Thr Pro Glu             500 505 510 Arg Leu Lys Arg Glu Val Met Gly Val Gln Trp Pro Cys Pro Ser Glu         515 520 525 Asp His Pro Gly Thr Pro Arg Leu His Val Glu Arg Phe Ala Thr Pro     530 535 540 Asp Gly Lys Ala Asn Ile Ile Pro Val Glu Phe Lys Pro Pro Ala Glu 545 550 555 560 Glu Pro Asp Glu Glu Tyr Pro Phe Ile Leu Thr Thr Phe Arg Ile Val                 565 570 575 Gly Gln Tyr His Thr Leu Thr Met Ser Asn Arg Ser Glu Ser Leu Lys             580 585 590 Lys Arg Trp Ser Ser Pro Tyr Ala Gln Ile Ser Pro Glu Asp Ala Lys         595 600 605 Lys Leu Gly Ile Gln Asp Gly Glu Met Ile Arg Ile Val Thr Arg Arg     610 615 620 Gly Ser Tyr Thr Cys Arg Ala Val Val Thr Glu Asp Val Ser Glu Gly 625 630 635 640 Val Ile Ala Val Pro Trp His Trp Gly Ala Asn Ile Leu Thr Asn Asp                 645 650 655 Val Leu Asp Pro Glu Ala Lys Ile Pro Glu Leu Lys Val Ala Ala Cys             660 665 670 Arg Val Glu Lys Ile Gly Gly Cys         675 680 <210> 55 <211> 163 <212> PRT <213> Thermococcus onnurineus NA1 <400> 55 Met Glu Lys Lys Leu Phe Ile Asn Leu Gly Arg Cys Ile Ala Cys Arg   1 5 10 15 Ala Cys Glu Val Ala Cys Glu Lys Glu His Gly Ile Ser Phe Ile Thr              20 25 30 Val Tyr Glu Phe Arg Asp Ile Ala Val Pro Leu Asn Cys Arg His Cys          35 40 45 Glu Lys Ala Pro Cys Ile Glu Val Cys Pro Thr Lys Ala Ile Tyr Arg      50 55 60 Asp Glu Asp Gly Ala Val Val Ile Asp Glu Ser Lys Cys Ile Gly Cys  65 70 75 80 Tyr Met Cys Ser Ala Val Cys Pro Tyr Ala Ile Pro Ile Val Asp Pro                  85 90 95 Ile Lys Glu Leu Ala Val Lys Cys Asp Leu Cys Ala Glu Arg Arg Lys             100 105 110 Glu Gly Arg Asp Pro Leu Cys Ala Ala Val Cys Pro Thr Asp Ala Ile         115 120 125 Ile Tyr Ala Asp Leu Asn Glu Leu Met Glu Glu Lys Arg Arg Arg Lys     130 135 140 Ala Glu Arg Ile Val Glu Ala Gln Arg Lys Ala Val Glu Thr Leu Ala 145 150 155 160 Tyr Phe Gly             <210> 56 <211> 125 <212> PRT <213> Thermococcus onnurineus NA1 <400> 56 Val Leu Lys Val Glu Leu Cys Val Gly Cys Gly Val Cys Ala Lys Ala   1 5 10 15 Cys Pro His Ser Ala Ile Ser Val Phe Glu Asp Ser Val Arg Arg Ile              20 25 30 Val Phe Asp Pro Lys Lys Cys Glu Glu Cys Ser Phe Glu Cys Asn Glu          35 40 45 Ala Cys Pro Thr Gly Ala Leu Glu Gly Lys Ser Asp Lys Arg Glu Leu      50 55 60 Val Phe Glu Phe Ala Tyr Cys Ala Ile Cys Gly Lys Arg Leu Asn Ile  65 70 75 80 Val Lys Glu Glu Ala Glu Tyr Leu Ala Lys Lys Leu Ile Glu Leu Gly                  85 90 95 Glu Asn Pro Glu Ile Ala Phe Leu Cys Asp Asp Cys Lys Arg Lys Arg             100 105 110 Leu Phe Gly Val Ala Asn Lys Tyr Glu Ala Tyr Leu Gly         115 120 125 <210> 57 <211> 352 <212> PRT <213> Thermococcus onnurineus NA1 <400> 57 Met Ser Gly Met Arg Phe Ala Phe Leu Cys Arg Glu Arg Pro Glu Pro   1 5 10 15 Thr Gly Lys Lys Ile Ala Val Ile Gly Ala Gly Pro Ala Gly Leu Ala              20 25 30 Ala Thr Gly Tyr Leu Val Cys Gln Gly His Glu Val His Val Tyr Asp          35 40 45 Lys Leu Pro Glu Pro Gly Gly Leu Met Leu Phe Gly Ile Pro Glu Phe      50 55 60 Arg Ile Pro Ile Tyr Arg Val Arg Glu Gly Tyr Glu Glu Leu Glu Arg  65 70 75 80 Val Tyr Asn Val Lys Phe Phe Thr Arg Thr Lys Val Tyr Phe Gly Asn                  85 90 95 Leu Glu Gly Glu Ser Gly Asp Glu Phe Val Glu Asn Arg Val Asp Phe             100 105 110 Lys Glu Leu Val Glu Lys Tyr Asp Ala Val Leu Ile Ala Thr Gly Thr         115 120 125 Trp Lys Cys Trp Ile Pro Asn Ile Glu Gly Ala Glu Leu Glu Gly Val     130 135 140 Phe Pro Ala Leu Glu Tyr Leu Phe Arg Ile Lys Ser Ala Lys Leu Gly 145 150 155 160 His Met Asp Trp Gly Lys Val Thr Pro Val Glu Gly Lys Lys Val Leu                 165 170 175 Val Val Gly Ala Gly His Thr Ala Val Asp Ala Ala Leu Glu Ser Val             180 185 190 Leu Leu Gly Ala Asp Lys Val Tyr Leu Ser Tyr Arg Arg Thr Ile Arg         195 200 205 Glu Ala Pro Ala Gly Ala Tyr Glu Ile Asn Leu Leu Gln Gln Arg Gly     210 215 220 Val Lys Trp Leu Glu Arg Thr Met Pro Val Arg Ile Ile Gly Glu Asn 225 230 235 240 Gly Lys Val Arg Ala Val Glu Leu Val Lys Thr Lys Leu Ser Glu Pro                 245 250 255 Asp Glu Ser Gly Arg Arg Arg Pro Val Pro Ile Glu Gly Ser Asn Phe             260 265 270 Gln Ile Asp Val Asp Tyr Val Ile Phe Ala Val Gly Gln Ser Pro Thr         275 280 285 Pro Pro Phe Ala Glu Glu Ile Asp Ile Ala Val Asp Lys Lys Gly Arg     290 295 300 Ile Val Val Asp Asn Arg His Met Thr Ser Arg Glu Gly Val Phe Ala 305 310 315 320 Ala Gly Asp Val Val Leu Gly Pro Ser Lys Val Gly Lys Ala Val Lys                 325 330 335 Asp Gly Leu Tyr Ala Ala Glu Ala Met His Met Trp Leu Met Gly Arg             340 345 350 <210> 58 <211> 168 <212> PRT <213> Thermococcus onnurineus NA1 <400> 58 Met Thr Arg Arg Ile Leu His Val Asp Tyr Ser Leu Cys Ile Gly Cys   1 5 10 15 Glu Thr Cys Glu Ala Val Cys Asp Phe Leu His Gly Gly Lys Pro Asn              20 25 30 Ile Arg Ile Tyr Tyr Thr Val Thr Gly Leu Pro Ile Pro Ile Asn Cys          35 40 45 Arg His Cys Glu Arg Ala Pro Cys Met Asp Val Cys Pro Ala Gly Ala      50 55 60 Ile Tyr Arg Asp Ser Asp Gly Ala Ile Ile Ile Asn Pro Asp Lys Cys  65 70 75 80 Ile Gly Cys Tyr Met Cys Leu Ala Val Cys Pro Phe Gly Val Pro Ser                  85 90 95 Phe Asp Val Lys Thr Lys Ala Val Thr Lys Cys Asp Met Cys Ala Asp             100 105 110 Arg Arg Arg Leu Gly Met Glu Pro Ala Cys Ala Glu Met Cys Pro Ala         115 120 125 Glu Ala Ile Phe Phe Gly Lys Pro Glu Glu Val Glu Asp Arg Ile Arg     130 135 140 Arg Arg Thr Ala Glu Arg Ile Ala Arg Glu Arg Ile Ala Ala Val Asp 145 150 155 160 Met Glu Gly Val Gly Arg Met Leu                 165 <210> 59 <211> 406 <212> PRT <213> Thermococcus onnurineus NA1 <400> 59 Met Leu Trp Glu Ser Gln Ile Pro Ile Asn Gln Val Phe Glu Leu Arg   1 5 10 15 Cys Arg Ser Met Thr Tyr Phe Gly Val Gly Ala Ile Asn Lys Phe Tyr              20 25 30 Asp Ile Ala Lys Asp Leu Lys Glu Asn Arg Gly Ile Thr Lys Val Ile          35 40 45 Leu Val Thr Gly Lys Ser Ser Tyr Lys Lys Cys Gly Ala Trp Asp Val      50 55 60 Val Lys Pro Ala Leu Glu Glu Tyr Gly Ile Glu Tyr Val His Tyr Asp  65 70 75 80 Lys Val Gly Pro Asn Pro Thr Val Asp Met Ile Asp Glu Ala Thr Gln                  85 90 95 Leu Gly Lys Glu Phe Gly Ala Gln Ala Val Ile Gly Ile Gly Gly Gly             100 105 110 Ser Pro Ile Asp Ser Ala Lys Ser Val Ala Ile Leu Leu Glu Tyr Thr         115 120 125 Asp Lys Thr Ala Arg Asp Leu Tyr Glu Leu Lys Phe Thr Pro Thr Lys     130 135 140 Ala Lys Pro Ile Ile Ala Val Asn Thr Thr His Gly Thr Gly Thr Glu 145 150 155 160 Val Asp Arg Phe Ala Val Ala Ser Ile Pro Glu Lys Glu Tyr Lys Pro                 165 170 175 Ala Ile Ala Tyr Asp Cys Ile Tyr Pro Leu Tyr Ser Ile Asp Asp Pro             180 185 190 Ala Leu Met Thr Lys Leu Pro Ala Asp Gln Thr Arg Tyr Val Thr Ile         195 200 205 Asp Ala Leu Asn His Ile Thr Glu Ala Ala Thr Thr Lys Phe Ala Ser     210 215 220 Pro Tyr Ser Ile Leu Leu Ala Gln Glu Thr Ala Arg Leu Ile Phe Asp 225 230 235 240 Tyr Leu Pro Glu Ala Leu Ala His Pro Asp Asn Leu Gln Ala Arg Tyr                 245 250 255 Tyr Leu Leu Tyr Ala Ser Ala Ile Ala Gly Ile Ser Phe Asp Asn Gly             260 265 270 Leu Leu His Phe Thr His Ala Leu Glu His Pro Leu Ser Ala Val Lys         275 280 285 Pro Asp Leu Pro His Gly Leu Gly Leu Ala Met Leu Leu Pro Ala Val     290 295 300 Ile Lys His Ile Tyr Pro Ala Thr Ala Arg Ile Leu Ala Glu Val Tyr 305 310 315 320 Arg Pro Leu Val Pro Glu Ala Lys Gly Val Pro Gly Glu Ala Glu Leu                 325 330 335 Val Ala Lys Lys Val Glu Glu Trp Leu Phe Asn Ile Gly Ile Thr Gln             340 345 350 Lys Leu Ile Asp Val Gly Phe Thr Glu Glu Asp Val Asp Lys Leu Ala         355 360 365 Glu Leu Ala Met Thr Thr Pro Ser Leu Asp Leu Leu Leu Ser Leu Ala     370 375 380 Pro Ile Glu Ala Thr Lys Glu Thr Val Ala Ala Ile Tyr Arg Asp Ser 385 390 395 400 Leu Tyr Pro Leu Asn Lys                 405 <210> 60 <211> 367 <212> PRT <213> Thermococcus onnurineus NA1 <400> 60 Met Gly Val Ile Val Val Gln Pro Ser Gly Arg Cys Asp Ala Thr Cys   1 5 10 15 Ala Asn Cys Ile Trp Arg Glu Arg Leu Ser Gly Val Met Leu Pro Gly              20 25 30 Asp Val Leu Pro Arg Ile Ala Ser Leu Leu Asp Gly Phe Arg Phe Asp          35 40 45 Glu Gly Ile Leu Met Cys Pro Asn Pro Phe Leu His Pro Lys Ile Lys      50 55 60 Ile Ile Tyr Asp Glu Leu Arg Asp Ile Ser Lys Arg Val Thr Val Phe  65 70 75 80 Ile Pro Leu Thr Ala Ser Leu Ser Asn Leu Arg Val Asp Val Leu Ala                  85 90 95 Asp Val Asp Met Ile Ser Ile Ile Val Pro Pro Met Ile Asp Ile Lys             100 105 110 Arg Gly Asp Thr Leu Ile Arg Ala Leu Glu Ser Arg Gly Ile Asp His         115 120 125 Ile Glu Ala Tyr Leu Val Phe Asn Ser Ser Ser Asp Pro Gly Glu Ile     130 135 140 Leu Arg Lys Ile Gly Glu Cys Met Lys Arg Gly Leu Arg Ile Thr Val 145 150 155 160 Gly Pro Ser Leu Phe Ser Pro Pro Ser Gly Asp Met Phe Ile Glu Ser                 165 170 175 Ile Ser Ala Arg Lys Asp Val Glu Leu Gly Leu His Tyr Gly Arg Lys             180 185 190 Tyr Leu Tyr Ser Ala Met Lys Val Phe Leu Asn Asp Tyr Pro Ile Thr         195 200 205 Leu Leu Met Ser Pro Met Asp Pro Cys Arg His Leu Tyr Val Asn Pro     210 215 220 Tyr Gly Ile Ile Ser Lys Cys Pro Asn Ser Asn Phe Ser Val Ser Tyr 225 230 235 240 Arg Glu Met Thr Arg Glu Leu Leu Arg Lys Ile Phe Phe Ser Pro Cys                 245 250 255 Pro Asn Asn Lys Asn Pro Ser Phe Val Pro Lys Val Glu Ile Ser Phe             260 265 270 Val Thr Ser Ser Gly Ile Lys Ile Pro Gly Asp Ile Met Glu Leu Leu         275 280 285 Glu Leu Ile Ser Gln Thr Arg Ser Phe Arg Ala Ala Cys Lys Ile Met     290 295 300 Gly Val Ser Pro Ser Thr Tyr Trp Glu Arg Ile Arg Asp Ile Glu Glu 305 310 315 320 Lys Leu Gly Arg Arg Leu Ile Val Ser Val Lys Gly Gly Arg Lys Lys                 325 330 335 Gly Ile Thr Val Leu Thr Gly Val Ala Leu Asp Leu Leu Lys Glu Tyr             340 345 350 Gln Arg Ile Arg Glu Arg Val Leu Leu Ser Leu Asn Glu Arg Phe         355 360 365 <210> 61 <211> 212 <212> PRT <213> Thermococcus onnurineus NA1 <400> 61 Met Pro Ala Phe Ser Gly Ser Asn Met Glu Lys Leu Thr Ile Tyr Ile   1 5 10 15 Asn Pro Glu Arg Cys Thr Gly Cys Arg Ala Cys Glu Ile Ala Cys Ala              20 25 30 Val Glu His Ser Met Ser Lys Asn Leu Phe Gly Ala Ile Phe Glu Lys          35 40 45 Pro Thr Pro Lys Pro Arg Leu Gln Val Val Val Ala Asp Phe Phe Asn      50 55 60 Val Pro Met Arg Cys Gln His Cys Glu Asp Ala Pro Cys Met Glu Ala  65 70 75 80 Cys Pro Thr Gly Ala Ile Ser Arg Thr Lys Glu Gly Phe Val Val Leu                  85 90 95 Asn Ala Asn Lys Cys Ile Gly Cys Leu Met Cys Val Met Ala Cys Pro             100 105 110 Phe Gly His Pro Lys Phe Glu Pro Glu Tyr Lys Ala Val Ile Lys Cys         115 120 125 Asp Ser Cys Val Asp Arg Val Arg Glu Gly Lys Glu Pro Ala Cys Val     130 135 140 Glu Ala Cys Pro Thr Arg Ala Leu Lys Phe Gly Thr Leu Gly Glu Ile 145 150 155 160 Leu Glu Glu Val Arg Lys Glu Lys Ala Glu Ser Leu Ile Ser Gly Leu                 165 170 175 Lys Ser Gln Gly Met Val Tyr Met Lys Pro Val Ser Glu Ser Lys Lys             180 185 190 Lys Glu Asp Leu Val Arg Pro Met Asp Leu Tyr Leu Ala Tyr Ser Asn         195 200 205 Val Val Trp Tyr     210 <210> 62 <211> 630 <212> PRT <213> Thermococcus onnurineus NA1 <400> 62 Met Ala Gly Lys Lys Val Pro Ser Lys Gln Val Ser Ile Thr Pro Gly   1 5 10 15 Val Gly Lys Leu Ile Glu Lys Ala Glu Glu Asp Gly Val Lys Thr Ala              20 25 30 Trp His Arg Phe Leu Glu Gln Gln Pro Gln Cys Gly Phe Gly Leu Leu          35 40 45 Gly Val Cys Cys Lys Asn Cys Thr Met Gly Pro Cys Arg Ile Asp Pro      50 55 60 Phe Gly Val Gly Pro Thr Lys Gly Val Cys Gly Ala Asp Ala Asp Thr  65 70 75 80 Ile Val Ala Arg Asn Ile Val Arg Met Ile Ala Ala Gly Thr Ala Gly                  85 90 95 His Ser Asp His Ser Arg Asp Val Val His Val Phe Lys Gly Ile Ala             100 105 110 Glu Gly Lys Phe Lys Asp Tyr Lys Leu Thr Asp Val Glu Lys Leu Lys         115 120 125 Glu Leu Ala Lys Ile Leu Gly Val Glu Thr Glu Gly Lys Ser Glu Asn     130 135 140 Glu Ile Ala Leu Glu Val Ala His Ile Leu Glu Met Glu Phe Gly Lys 145 150 155 160 Gln Asp Glu Glu Pro Val Arg Leu Leu Ala Ala Thr Ala Pro Lys Lys                 165 170 175 Arg Ile Lys Val Trp Glu Lys Leu Gly Val Leu Pro Arg Ala Ile Asp             180 185 190 Arg Glu Ile Cys Leu Ser Met His Arg Thr His Ile Gly Cys Asp Ala         195 200 205 Asp Pro Ala Ser Leu Leu Leu His Gly Val Arg Thr Ala Leu Ala Asp     210 215 220 Gly Trp Cys Gly Ser Met Met Ala Thr Tyr Leu Ser Asp Ile Leu Phe 225 230 235 240 Gly Thr Pro Lys Pro Ile Lys Ser Leu Ala Asn Leu Gly Val Leu Lys                 245 250 255 Glu Asp Met Val Asn Ile Ile Val His Gly His Asn Pro Ile Leu Ser             260 265 270 Met Lys Ile Ala Glu Ile Ala Gln Ser Glu Glu Met Gln Lys Leu Ala         275 280 285 Glu Gln Tyr Gly Ala Lys Gly Ile Asn Val Ala Gly Met Cys Cys Thr     290 295 300 Gly Asn Glu Val Leu Ser Arg Met Gly Val Gln Val Ala Gly Asn Phe 305 310 315 320 Leu Met Gln Glu Leu Ala Ile Ile Thr Gly Ala Val Glu Ala Val Ile                 325 330 335 Val Asp Tyr Gln Cys Leu Met Pro Ser Leu Val Asp Val Ala Ser Cys             340 345 350 Tyr His Thr Lys Ile Ile Thr Thr Glu Pro Lys Ala Arg Ile Pro Gly         355 360 365 Ala Ile His Val Glu Phe Glu Pro Glu Lys Ala Asp Glu Ile Ala Lys     370 375 380 Glu Ile Ile Lys Ile Ala Ile Glu Asn Tyr Lys Asn Arg Val Pro Ala 385 390 395 400 Lys Val Tyr Ile Pro Glu His Lys Met Glu Leu Val Ala Gly Phe Ser                 405 410 415 Val Glu Ala Ile Leu Glu Ala Leu Gly Gly Thr Leu Glu Pro Leu Ile             420 425 430 Lys Ala Leu Gln Asp Gly Thr Ile Lys Gly Ile Val Gly Ile Val Gly         435 440 445 Cys Asn Asn Pro Arg Val Lys Gln Asn Tyr Gly His Val Thr Leu Ala     450 455 460 Lys Glu Leu Ile Lys Arg Asp Ile Leu Val Val Gly Thr Gly Cys Trp 465 470 475 480 Gly Ile Ala Ala Ala Met His Gly Leu Leu Thr Pro Glu Ala Ala Glu                 485 490 495 Met Ala Gly Pro Gly Leu Lys Ala Val Cys Glu Ala Leu Gly Ile Pro             500 505 510 Pro Cys Leu His Met Gly Ser Cys Val Asp Cys Ser Arg Ile Leu Leu         515 520 525 Val Leu Ser Ala Leu Ala Asn Ala Leu Asn Val Asp Ile Ser Asp Leu     530 535 540 Pro Val Ala Gly Ser Ala Pro Glu Trp Met Ser Glu Lys Ala Val Ala 545 550 555 560 Ile Gly Thr Tyr Phe Val Ala Ser Gly Val Phe Thr His Leu Gly Val                 565 570 575 Ile Pro Pro Val Leu Gly Ser Gln Lys Val Thr Lys Leu Leu Thr Asp             580 585 590 Asp Ile Glu Asp Leu Leu Gly Gly Lys Phe Tyr Val Glu Thr Asp Pro         595 600 605 Val Lys Ala Ala Glu Thr Ile Tyr Asn Val Ile Ile Glu Lys Arg Lys     610 615 620 Lys Leu Gly Trp Pro Ile 625 630 <210> 63 <211> 269 <212> PRT <213> Thermococcus onnurineus NA1 <400> 63 Met Lys Ile Leu Val Ala Gly Lys Gly Gly Val Gly Lys Thr Thr Thr   1 5 10 15 Thr Ala Leu Leu Ala His Ile Ile Ala Asp Lys Gly Tyr Asn Val Leu              20 25 30 Thr Leu Asp Thr Asp Ser Val Pro Asn Leu Ala Gln Ser Leu Gly Val          35 40 45 Pro Tyr Asp Glu Ala Leu Asp Ile Val Pro Leu Ser Arg Asn Glu Glu      50 55 60 Leu Ala Gln Glu Arg Thr Gly Ala Lys Pro Gly Ser Gly Trp Gly Val  65 70 75 80 Leu Phe Ser Leu Thr Pro Lys Val Asp Asp Leu Val Asp Met Tyr Gly                  85 90 95 Ile Arg Ile Lys Pro Asn Leu Ser Leu Val Val Val Gly Ser Thr Glu             100 105 110 Gln Pro Lys Glu Gly Cys Leu Cys Pro Ser Ile Ala Leu Ala Lys Ala         115 120 125 Phe Leu Asn His Val Leu Leu Arg Glu Lys Asp Ile Val Ile Val Asp     130 135 140 Ser Glu Ala Gly Ala Glu Val Phe Gly Arg Gly Leu Ala Glu Lys Phe 145 150 155 160 Asp Val Met Leu Cys Val Ala Glu Pro Thr Leu Lys Ser Leu Thr Ile                 165 170 175 Ala Lys Lys Leu Leu Lys Met Gly Glu Glu Leu Asn Ile Ser Asn Leu             180 185 190 Met Leu Ile Ile Asn Lys Val His Asp Ser Met Glu Ala Ala Lys Leu         195 200 205 Tyr Ser Arg Val Phe Ser Asp Tyr Leu Val Pro Tyr His Leu Val His     210 215 220 Phe Asp Thr Ser Val Ile Lys Ala Asp Asn Glu Gly Leu Gly Val Asn 225 230 235 240 Ser Ile Pro Arg Asp Ser Arg Ile Tyr Arg Asp Val Glu Ser Leu Ala                 245 250 255 Asn Lys Leu Leu Ser Met Lys Lys Arg Ser Lys Val Val             260 265 <210> 64 <211> 64 <212> PRT <213> Thermococcus onnurineus NA1 <400> 64 Met Cys Glu Ser Lys Ala Val Val Val Glu Asn Gly Lys Glu Glu Ile   1 5 10 15 Ile Met Glu Asp Val Ala Phe Leu Tyr Phe Lys Glu Gly Lys Pro Val              20 25 30 Ile Arg Asp Ile Leu Gly Arg Glu Met Leu Leu Glu Asp Tyr Glu Leu          35 40 45 Asp Ser Ile Asp Phe Leu Arg His Ile Met Arg Phe Lys Leu Arg Arg      50 55 60 <210> 65 <211> 634 <212> PRT <213> Thermococcus onnurineus NA1 <400> 65 Met Glu Val Ile Phe Leu Phe Ile Val Ile Ile Leu Ser Val Ala Ser   1 5 10 15 Phe Ile Gly Val Phe Ser Arg Ser Ala Ile Leu Thr Lys Leu Val Asn              20 25 30 Ala Leu Ser Ala Leu Gly Ser Leu Thr Ile Ala Tyr Ala Gly Ile Val          35 40 45 Gly Leu Lys Glu Ser Val Glu Leu Asn Ile Thr Leu Leu His Leu Lys      50 55 60 Ser Asp Ser Ile Ile Asn Ala Phe Ser Thr Leu Thr Leu Lys Val Asp  65 70 75 80 Pro Leu Ser Gly Phe Phe Met Ile Ile Leu Gly Ile Leu Gly Phe Cys                  85 90 95 Thr Ser Val Tyr Gly Ile Ala Tyr Leu Asp Met Tyr Lys Gly Asp Lys             100 105 110 Arg Leu Tyr Ala Phe Asn Tyr Pro Leu Phe Leu Leu Phe Met Phe Leu         115 120 125 Val Leu Val Ser Trp Asn Leu Leu Trp Phe Val Val Phe Trp Glu Leu     130 135 140 Met Thr Leu Phe Ser Gln Phe Leu Val Ala Phe Glu Arg Asn Glu Lys 145 150 155 160 Thr Leu Ile Ala Thr Leu Lys Tyr Phe Cys Met Thr Lys Ala Ala Ala                 165 170 175 Asp Phe Met Leu Ile Ala Ile Val Leu Val Leu Ile Thr Ile Ser Gly             180 185 190 Gly Gly Asp Tyr Asp Ile Leu Ser Ser Gln Leu Val Asn Tyr Phe Arg         195 200 205 Ser His Pro Leu Glu Met Tyr Leu Val Ser Ala Gly Phe Met Ile Gly     210 215 220 Leu Gly Val Lys Ala Ala Leu Val Pro Phe His Val Trp Leu Pro Asp 225 230 235 240 Ala Tyr Val Glu Ala Pro Ser Asn Val Ser Ser Leu Leu Ser Gly Ala                 245 250 255 Met Glu Lys Met Pro Val Tyr Met Met Phe Arg Phe Phe Leu Ser Phe             260 265 270 Thr Pro Leu Thr Pro Asn Ile Gly Leu Leu Ile Ala Leu Phe Gly Thr         275 280 285 Leu Thr Leu Phe Phe Gly Thr Met Tyr Ala Leu Lys Gln Thr Asp Ser     290 295 300 Lys Arg Leu Leu Ala Tyr His Ser Val Gly Gln Ile Gly Tyr Val Val 305 310 315 320 Phe Ala Leu Gly Ala Gly Ile Tyr Leu Leu Ser Lys Gly Tyr Thr Thr                 325 330 335 Phe Gly Ala Leu Ala Leu Met Ala Ser Leu Phe His Ala Leu Asn His             340 345 350 Ala Phe Phe Lys Gly Leu Leu Phe Leu Thr Ala Gly Ser Ile Leu Tyr         355 360 365 Arg Thr Gly Ser Arg Asp Leu Asp His Leu Gly Gly Leu Ala Arg Phe     370 375 380 Met Pro Ile Thr Ala Phe Ala Ala Leu Ile Gly Ser Leu Ser Ile Ala 385 390 395 400 Gly Met Pro Pro Phe Asn Gly Phe Val Ser Lys Trp Met Ile Tyr Val                 405 410 415 Ser Thr Leu Pro Thr Pro Thr Leu Val Ser Leu Phe Gly Ala Leu Ala             420 425 430 Leu Phe Ile Ser Ala Val Thr Thr Ala Ser Phe Val Lys Tyr Phe Thr         435 440 445 Ser Ile Phe Val Arg Pro Pro Ala Lys Glu Ile Thr Val Lys Glu Val     450 455 460 Pro Val Ser Met Trp Ala Ser Gln Leu Ile Leu Ala Val Leu Cys Val 465 470 475 480 Ile Phe Gly Val Tyr Pro Ala Leu Pro Leu Glu Ala Ile Ser Lys Ala                 485 490 495 Val Asp Ser Val Gly Val Thr Thr Pro Ser Ile Thr Val Phe Pro Gly             500 505 510 Leu Ile Val Ser Asp Gly Ile Gly Asn Ile Ala Pro Leu Ala Leu Leu         515 520 525 Val Phe Ser Gly Ala Leu Thr Ala Val Leu Leu Ala Ile Phe Pro Tyr     530 535 540 Lys Ile Ser Leu Pro Val Trp Thr Thr Gly Thr Arg Arg Ser Leu Ala 545 550 555 560 Met Arg Leu Pro Ala Ser Ser Tyr Tyr Ala Ser Phe Glu Glu Glu Phe                 565 570 575 Glu Asp Val Tyr Ser Trp Gly Glu Trp Cys Val Cys Thr Thr Lys Arg             580 585 590 Leu Trp Asp Ala Thr Lys Ala Val Leu Ser Asn Phe Glu Glu Val Ser         595 600 605 Phe Asp Leu Asp Lys Met Met Thr Gly Ala Trp Leu Met Leu Leu Ile     610 615 620 Leu Leu Thr Ile Leu Gly Gly Val Leu Leu 625 630 <210> 66 <211> 293 <212> PRT <213> Thermococcus onnurineus NA1 <400> 66 Met Asn Ala Val Tyr Ala Ala Leu Asn Leu Ile Phe Ile Val Leu Phe   1 5 10 15 Ala Pro Leu Leu Asp Gly Ile Glu Arg Lys Val Lys Ala Arg Leu Gln              20 25 30 Ser Arg Gln Gly Pro Pro Leu Ile Gln Thr Trp Leu Asp Leu Leu Lys          35 40 45 Leu Phe Arg Arg Pro Asn Val Arg Pro Arg Glu Ser Val Arg Trp Leu      50 55 60 Phe Glu Pro Ala Pro Ala Ile Ala Leu Val Ser Val Leu Ala Ala Ser  65 70 75 80 Leu Phe Ile Pro Ser Leu Leu Pro Gly Ser Leu Asp Thr Trp Gly Asp                  85 90 95 Ile Ile Ala Phe Ile Tyr Leu Ser Thr Leu Ser Ala Val Ala Ile Ala             100 105 110 Leu Gly Ala Phe Ser Thr Gly Ser Pro Tyr Ala Gln Ile Gly Ser His         115 120 125 Arg Glu Val Ser Ile Ile Met Ala Glu Glu Phe Ser Leu Ala Phe Ile     130 135 140 Val Ala Ala Leu Ala Ala Ser Ser Gly Gly Leu Ser Phe Ser Arg Leu 145 150 155 160 Phe Pro Leu Gln Leu Lys Val Ser Thr Ile Thr Gly Ala Leu Ala Phe                 165 170 175 Ala Val Met Ala Tyr Val Ala Gly Ala Arg Ile Pro Phe Asp Val Ala             180 185 190 Glu Ala Glu Pro Glu Ile Val Glu Gly Pro Phe Ile Glu Phe Ser Gly         195 200 205 Lys Gly Leu Gly Met Leu Lys Leu Ser Ile Tyr Val Lys Arg Leu Leu     210 215 220 Leu Thr Thr Ile Leu Leu Asn Phe Phe Leu Pro Gln Asp Gly Thr Val 225 230 235 240 Arg Val Leu Val Tyr Val Ile Gly Leu Val Ile Ile Ser Val Val Tyr                 245 250 255 Ala Ser Ile Glu Ala His Tyr Gly Arg Phe Arg Thr Lys Asp Ala Ala             260 265 270 Arg Phe Leu Lys Arg Phe Ala Ile Val Gly Ile Leu Ser Trp Ile Leu         275 280 285 Gly Val Val Gly Trp     290 <210> 67 <211> 540 <212> PRT <213> Thermococcus onnurineus NA1 <400> 67 Met Val Phe Asp Ile Leu Lys Gly Cys Lys Ile Leu Glu His Asn Asp   1 5 10 15 Lys Met Thr Val Ala Glu Val Gly Ala Ser Asn Ile Arg Glu Ile Ala              20 25 30 Arg Ala Leu Phe Glu Arg Gly Tyr Tyr Tyr Ser Ser Gly Met Gly Val          35 40 45 Asp Glu Arg Pro Ile Asn Gly Arg Phe Ala Met Tyr His Ile Phe Asn      50 55 60 Cys Asp Thr Glu Gly Arg Tyr Val Val Leu Lys Ile Thr Ser Pro Glu  65 70 75 80 Gly Ser Pro Glu Val Pro Ser Ile Thr Pro Val Ile Lys Gly Ala Glu                  85 90 95 Trp Ser Glu Arg Glu Ala Met Asp Met Leu Gly Ile Val Phe Ser Gly             100 105 110 His Pro Lys Pro Glu Arg Leu Ile Leu Pro Asp Asp Trp Pro Glu Gly         115 120 125 Val Tyr Pro Leu Arg Lys Asp Phe Pro Tyr Asn Lys Lys Leu Pro Pro     130 135 140 Ser Lys Pro Ile Glu Lys Glu Arg Glu His Lys Lys Asp Val Met Glu 145 150 155 160 Ile Pro Leu Gly Pro Tyr His Pro Ser Leu His Glu Pro Glu Tyr Phe                 165 170 175 Glu Leu Tyr Val Lys Gly Asp Lys Val Val Asp Ala Glu Tyr Arg Gly             180 185 190 Phe His Ile His Arg Gly Met Glu Lys Leu Ala Glu Ser Arg Met Thr         195 200 205 Ile Asn Gln Ile Pro Phe Leu Ala Glu Arg Ile Cys Gly Ile Cys Gly     210 215 220 Cys Thr His Ser Ala Ala Tyr Cys Gln Ala Val Glu Asp Ala Ala Gly 225 230 235 240 Ile Tyr Val Pro Glu Arg Ala Gln Tyr Ile Arg Thr Ile Met Leu Glu                 245 250 255 Val Glu Arg Ile His Ser His Leu Leu Trp Phe Gly Val Val Cys His             260 265 270 Leu Leu Gly Phe Asp Ser Gly Phe Met His Ile Trp Arg Ala Arg Glu         275 280 285 Tyr Ile Met Asp Ile Ala Glu Leu Ile Thr Gly Asn Arg Lys Thr Tyr     290 295 300 Gly Ile Asn Ile Val Gly Gly Val Arg Arg Asp Ile Thr Glu Asp Lys 305 310 315 320 Lys Glu Lys Thr Leu Lys Leu Leu Asp Met Val Glu Lys Glu Ser Arg                 325 330 335 Glu Val Leu Asp Asn Ile Ala Glu Met Lys Glu Leu Arg Glu Arg Met             340 345 350 Glu Gly Val Gly Val Leu Pro Lys Lys Glu Ala Arg Glu Ile Gly Val         355 360 365 Val Gly Pro Met Ala Arg Ser Ser Gly Ile Asp Thr Asp Val Arg Arg     370 375 380 Asp His Pro Tyr Ala Ala Tyr Lys Asp Leu Asp Phe Lys Val Pro Val 385 390 395 400 Tyr Lys Glu Gly Asp Val Phe Ala Arg Phe Leu Val Arg Tyr Glu Glu                 405 410 415 Ile Phe Glu Ser Phe Asn Met Ile Arg Gln Ala Leu Glu Asn Met Pro             420 425 430 Pro Gly Glu Leu Ile Asn Asp Glu Tyr Glu Ile Pro Pro Phe Lys Leu         435 440 445 Gly Ile Gly Val Thr Glu Ala Pro Arg Gly Glu Asn Ile His Ala Val     450 455 460 Ile Thr Trp Gly Glu Asn Met Ile Tyr Arg Trp His Pro Arg Ala Ala 465 470 475 480 Thr Tyr Asn Asn Leu Pro Ala Val Pro Ile Met Leu Arg Gly Asn Asp                 485 490 495 Val Ala Asp Ala Pro Leu Ile Ile Ala Ser Ile Asp Pro Cys Phe Ser             500 505 510 Cys Thr Asp His Val Ser Ile Ile Asp Ser Glu Ser Gly Lys Ile Leu         515 520 525 Trp Arg Gly Pro Leu Lys Glu Gly Val Arg Arg Val     530 535 540 <210> 68 <211> 191 <212> PRT <213> Thermococcus onnurineus NA1 <400> 68 Met Val Lys Asn Ser Leu Trp Val Phe His Leu Asn Ser Gly Ser Cys   1 5 10 15 Asn Gly Cys Asp Ile Glu Ile Leu Asn Ile Phe Ala Pro Arg Asn Asp              20 25 30 Val Glu Arg Leu Gly Ile Lys Leu Val Gly Ser Pro Arg His Ala Asp          35 40 45 Ala Ile Ala Phe Thr Gly Pro Ile Thr Arg Glu Cys Leu Pro Lys Val      50 55 60 Ile Asp Ala Leu Lys Ala Val Pro Glu Pro Lys Val Val Leu Ala Ile  65 70 75 80 Gly Ala Cys Ala Cys Gly Gly Gly Ile Trp Tyr Asp Thr Tyr Ser Val                  85 90 95 Ile Gly Gly Val Lys Glu Leu Tyr Arg Ile Leu Lys Glu Glu Tyr Asn             100 105 110 Met Glu Pro Pro Ala Thr Val Phe Ile Pro Gly Cys Pro Pro Lys Pro         115 120 125 Glu Ala Ile Ile Tyr Gly Val Ala Val Ala Ser Gly Met Leu Glu Ser     130 135 140 Lys Gln Lys Lys Thr Val Tyr Val Glu Pro Glu Glu Ser Val Ala Asn 145 150 155 160 Glu Lys Leu Met Ile Ala Glu Leu Ile Ser Glu Thr Glu Lys Thr Arg                 165 170 175 His Phe Met Pro Gly Ile Val Ile Arg Gly Val Glu Asp Glu Pro             180 185 190 <210> 69 <211> 94 <212> PRT <213> Thermococcus onnurineus NA1 <400> 69 Met Ser Leu Glu Asp Thr Phe Ile Ile Phe Met Ly Les Phe Ile Ile Pro   1 5 10 15 Leu Tyr Leu Leu Ala Phe Val Ile Tyr Ala Ile Arg Ala Phe Arg Gly              20 25 30 Pro Thr Ile Pro Asp Ile Ile Leu Ala Ile Asp Cys Met Ser Phe Asp          35 40 45 Ile Ala Ala Phe Met Ala Ile Leu Ala Val Tyr Phe Lys Ser Val Phe      50 55 60 Leu Ile Ser Gly Ala Ile Thr Leu Ala Leu Trp Ala Tyr Leu Leu Asp  65 70 75 80 Ile Tyr Ile Ala Lys Tyr Leu Val Ser Arg Glu Val Gly Ala                  85 90 <210> 70 <211> 119 <212> PRT <213> Thermococcus onnurineus NA1 <400> 70 Met Ser Glu Val Leu Phe Tyr Leu Gly Ala Ile Met Ile Ile Ile Gly   1 5 10 15 Gly Ile Cys Asp Leu Phe Gly Ala Ile Gly Leu Leu Arg Phe Pro Asn              20 25 30 Phe Tyr Val Arg Leu His Ala Ala Thr Val Gly Thr Ile Gly Gly Ala          35 40 45 Val Val Pro Leu Phe Gly Val Gly Phe Leu Ala Leu Gly Ser Asn Phe      50 55 60 Leu Pro His Lys Tyr Ala Ile Ala Gly Ala Ser Phe Val Thr Gly Ile  65 70 75 80 Ile Val Leu Leu Ala Ala Pro Ala Gly Ala His Ala Leu Ala Tyr Ala                  85 90 95 Ala His Lys Ala Lys Leu Val Gln Trp Asn Pro Lys Cys Asp His Leu             100 105 110 Thr Glu Val Lys Lys His Asp         115 <210> 71 <211> 84 <212> PRT <213> Thermococcus onnurineus NA1 <400> 71 Met Ile Glu Val His Leu Leu Met Leu Ala Ile Thr Thr Ile Ile Gly   1 5 10 15 Leu Ile Phe Ser Tyr Leu Ala Ile Ile Glu Lys Asp Leu Leu Arg Ala              20 25 30 Ile Gly Phe Ser Ala Val Gln Ala Ile Ala Tyr Ala Ile Ala Phe Tyr          35 40 45 Ile Leu Met Ala Pro Asp Ile Val Leu Ala Tyr Val Ala Ile Ala Val      50 55 60 Gly Ile Tyr Ser Ala Leu Leu Val Phe Val Val Ser Lys Thr Gly Arg  65 70 75 80 Tyr Glu Val Val                 <210> 72 <211> 256 <212> PRT <213> Thermococcus onnurineus NA1 <400> 72 Met Lys Lys Leu Ile Pro Leu Val Ile Ile Leu Val Ala Ile Leu Gly   1 5 10 15 Leu Ala Tyr Tyr Ile Ala Pro Lys Leu Pro Gln Gln Thr Asp Val Arg              20 25 30 Pro Leu Gly Glu Phe Tyr Leu Gln Asn Ser Tyr Phe Gly Asp Tyr Ser          35 40 45 Ala Arg Ser Pro Glu Val Val Thr Ser Ile Leu Trp Asp Tyr Arg Gly      50 55 60 Val Asp Thr Leu Phe Glu Thr Ala Val Phe Phe Leu Ala Ile Gly  65 70 75 80 Ser Leu Thr Leu Phe Arg Leu Asn Lys Arg Gln Glu Lys Ala Ala Lys                  85 90 95 Gln Lys Thr Glu Glu Phe Thr Gly Gly Leu Thr Ile Val Val Lys Ser             100 105 110 Val Thr Lys Ile Ile Val Val Met Ile Leu Ala Val Ser Ala Ser Ile         115 120 125 Ala Leu His Gly His Leu Thr Pro Gly Gly Gly Phe Gln Gly Gly Ser     130 135 140 Ala Leu Ala Val Ala Pro Leu Leu Ile Ile Ala Ala Tyr Ser Lys Tyr 145 150 155 160 Thr Leu Glu Glu Asn Gly Leu Asp Lys Thr Arg Ala Leu Ile Leu Arg                 165 170 175 Ser Ile Gly Leu Leu Gly Ile Ala Leu Val Ala Leu Val Pro Leu Leu             180 185 190 Ser Gly Gly Phe Ile Met Gln Asn Gln Pro Val Phe Pro Ala Glu Ile         195 200 205 Asp Ser Gln Leu Ile Gly Gly Ser Leu Ile Tyr Tyr Asn Phe Phe Glu     210 215 220 Phe Leu Ala Val Gly Ala Gly Phe Thr Ala Val Phe Leu Leu Leu Ala 225 230 235 240 Ile Pro Glu Ser Ile Phe Lys Arg Phe Leu Arg Gly Asp Val Asp Glu                 245 250 255 <210> 73 <211> 134 <212> PRT <213> Thermococcus onnurineus NA1 <400> 73 Met Ser Ser Ala Val Pro Phe Ile Leu Ser Ile Ile Ile Leu Ser Leu   1 5 10 15 Ile Ala Thr Ile Met Ile Gly Leu Tyr Gly Ile Val Gly Arg Pro Asn              20 25 30 Leu Val Lys Lys Leu Ile Ala Leu Thr Ile Leu Gly Asp Thr Thr Asn          35 40 45 Leu Leu Val Val Leu Leu Gly Tyr Arg Ser Ile Tyr Pro Val Met Pro      50 55 60 Pro Ile Leu Pro Asp Leu Ser Lys Glu Thr Leu Ser Gln Phe Ile Ser  65 70 75 80 Thr Ala Val Asp Pro Leu Pro Gln Ala Leu Val Ile Thr Ala Val Val                  85 90 95 Ile Gly Met Ala Val Asn Val Leu Leu Ala Phe Ala Ile Ile Gln Ile             100 105 110 Tyr Arg Leu Tyr Gly Thr Val Asp Thr Arg Glu Leu Gln Gln Glu Leu         115 120 125 Leu Gly Gly Gly Glu Arg     130 <210> 74 <211> 161 <212> PRT <213> Thermococcus onnurineus NA1 <400> 74 Met Lys Gly Val Val Pro Thr Ala Thr Leu Ile Phe Ile Thr Tyr Leu   1 5 10 15 Ile Phe Thr Gly Ser Ala Ser Pro Phe Asp Leu Val Thr Gly Ile Ile              20 25 30 Val Ala Ile Ser Val Gly Leu Leu Ile Gly Arg Tyr Leu Val Gln Asn          35 40 45 Asp Thr Lys Ala Met Asn Pro Thr Arg Trp Leu Trp Thr Val Ile Tyr      50 55 60 Phe Ile Trp Tyr Met Leu Val Ala Glu Thr Lys Ser His Val Asp Val  65 70 75 80 Met Val Arg Thr Ile Thr Gly Lys Tyr Glu Pro Gly Ile Val Lys Val                  85 90 95 Pro Ile Gly Val Lys Thr Asp Tyr Ala Lys Thr Leu Val Ala Asn Ser             100 105 110 Ile Thr Asn Thr Pro Gly Thr Val Val Val Asp Leu Asp Asp Lys Tyr         115 120 125 Met Tyr Val Asn Trp Ile Asn Val Thr Thr Glu Asp Pro Glu Gln Val     130 135 140 Lys Gly Glu Ile Ser Ala Asp Phe Glu Lys Phe Ala Lys Arg Ile Phe 145 150 155 160 Glu     <210> 75 <211> 520 <212> PRT <213> Thermococcus onnurineus NA1 <400> 75 Met Asp Val Val Gly Ile Thr Pro Val Ile Pro Val Ile Phe Ala Phe   1 5 10 15 Ala Leu Pro Leu Phe Ser Ile Ile Ile Lys Gly Asn Arg Lys Ile Ile              20 25 30 Gln Ala Tyr Val Leu Ile Gly Thr Gly Leu Ala Leu Val Ser Thr Phe          35 40 45 Lys Leu Phe Gln Lys Thr Tyr Ala Ser Asp Arg Pro Leu Val Tyr Thr      50 55 60 Phe Gly Gly Trp Ser Ala Pro Val Gly Val Ile Tyr Glu Val Asp Lys  65 70 75 80 Leu Ser Ala Leu Ile Ala Leu Val Thr Ala Gly Leu Met Phe Leu Ile                  85 90 95 Ser Ile Tyr Ser Tyr Arg Tyr Leu Glu His Glu Asn Gly Leu Glu Trp             100 105 110 Tyr Tyr Thr Leu Tyr Leu Gly Leu Glu Ala Gly Leu Leu Gly Val Leu         115 120 125 Leu Thr Gly Asp Ala Phe Asn Leu Phe Val Met Ile Glu Val Thr Ser     130 135 140 Ile Ala Ala Tyr Ala Leu Val Met Phe Tyr Arg Asp Arg Gly Asp Ser 145 150 155 160 Val Tyr Ala Gly Leu Lys Tyr Ala Leu Ile Gly Ala Val Gly Thr Thr                 165 170 175 Met Tyr Phe Leu Ala Leu Gly Ile Leu Tyr Gly Ala Phe Gly Thr Val             180 185 190 Asn Leu Ala Asp Leu Thr Ala Lys Val His Gly Met Asp Phe Pro Leu         195 200 205 Thr Gly Ala Pro Val Gly Asp Ile Val Val Ala Ser Gly Val Ala Leu     210 215 220 Ala Leu Ala Thr Trp Ala Phe Leu Ile Lys Ala Ala Ile Val Pro Asn 225 230 235 240 His Phe Trp Leu Pro Glu Ala His Pro Ala Ala Pro Ser Pro Ile Ser                 245 250 255 Ala Ile Leu Ser Gly Leu Val Val Asn Val Gly Val Tyr Ser Leu Ile             260 265 270 Arg Phe Leu Tyr Thr Val Tyr Gly Gly Thr Leu Ile Pro Glu Leu Ala         275 280 285 Arg Ile Val Ser Val Leu Ser Asn Ile Val Val Val Leu Gly Ala Val     290 295 300 Ser Ser Leu Phe Gly Ala Ala Met Met Thr Val Gln Lys Asp Val Lys 305 310 315 320 Arg Leu Ile Ala Tyr Ser Thr Ile Met His Met Gly Tyr Leu Phe Met                 325 330 335 Ala Val Gly Leu Gly Thr Gln Leu Gly Leu Gln Ala Ala Leu Phe His             340 345 350 Leu Val Asn His Ala Ile Ala Lys Ala Leu Leu Phe Leu Ala Ala Gly         355 360 365 Ala Phe Ile His Ala Ile Gly Ser Arg Asn Val Asp Asp Leu Ala Gly     370 375 380 Leu Gly Lys Lys Met Pro Val Ala Thr Phe Ser Leu Ala Ile Ala Thr 385 390 395 400 Leu Ser Leu Val Gly Val Pro Pro Leu Asn Val Phe Phe Ser Lys Leu                 405 410 415 Leu Leu Phe Asn Ala Leu Met Glu Lys Gly Leu Gly Pro Ala Leu Ile             420 425 430 Leu Val Ile Ser Ser Val Ile Ala Leu Val Ala Tyr Val Arg Val Phe         435 440 445 His Ile Ile Trp Leu Gly Lys Pro Arg Lys Asp Ile Lys Ile Lys Glu     450 455 460 His Ala Ser Met Ser Leu Val Cys Leu Val Leu Ala Val Ile Cys Ile 465 470 475 480 Ile Val Gly Leu Ala Thr Pro Ile Ile Val Asp Gly Leu Ile Asn Pro                 485 490 495 Ala Ala Thr Gln Ala Met Asp Tyr Asn Ser Tyr Ile Asn Ala Val Leu             500 505 510 Asp Leu Ala Leu Lys Thr Ile Arg         515 520 <210> 76 <211> 392 <212> PRT <213> Thermococcus onnurineus NA1 <400> 76 Leu Ser Glu Ile Thr Leu Asn Lys Val Cys Arg Ile Ala Gly Glu Ala   1 5 10 15 Lys Leu Val Leu Tyr Glu Glu Asn Gly Thr Val Gln Asp Ala Leu Phe              20 25 30 Ile Ala Thr Ala Pro Ile Arg Gly Phe Glu Lys Leu Val Val Gly Lys          35 40 45 Asn Pro Leu Phe Ala Val Glu Ala Val Met Arg Ile Cys Gly Leu Cys      50 55 60 His Ala Ser His Gly Ile Ala Met Ser Glu Ala Ile Glu Asn Ala Ile  65 70 75 80 Gly Ile Ile Pro Pro Arg Asn Gly Ile Leu Met Arg Glu Ala Leu Gly                  85 90 95 Leu Val Asn Arg Ile Gln Ser His Met Leu Glu Phe Leu Met Val Ala             100 105 110 Gly Asp Leu Leu Ile Glu Glu Lys Arg Glu Glu Val Leu Phe Gln Leu         115 120 125 Met Asp Phe His Ala Lys Ile Ser Asp Tyr Leu Leu Lys Met Gly Gly     130 135 140 Ala Ala Thr His Pro Pro Asn Leu Thr Val Gly Gly Met Phe Ser Val 145 150 155 160 Pro Lys Trp Ser Val Phe Asn Asn Leu Lys Ala Arg Leu Pro Lys Leu                 165 170 175 Thr Gly Gln Trp Glu Glu Ile Ala His Leu Leu Thr Asp Glu Asp Ile             180 185 190 Gln Thr Glu Val Ala Asp Glu Leu Arg Glu Lys Lys Ala Glu Asn Asn         195 200 205 Tyr Leu Val Ser Ser Leu Phe Tyr Gly Asp Arg Phe Asn Ile Asn Ala     210 215 220 Glu Arg Ile Glu Thr Met Pro Tyr Tyr Glu Tyr Arg Lys Asp Asn Pro 225 230 235 240 His Ser Lys Glu Ser Thr Thr Leu Ile Ala Phe Tyr Gly Gly Glu Lys                 245 250 255 Val Glu Ala Gly Pro Arg Ala Arg Met Lys Val Tyr Arg Glu Phe Thr             260 265 270 Asp Ser Ser Leu Tyr Gly Leu His Thr Ala Arg Val Gln Asp Thr Thr         275 280 285 Leu Ala Leu Ile Arg Leu Glu Glu Ile Leu Asp Ser Ile Lys Met Asp     290 295 300 Glu Pro Phe Arg Thr Lys Asn Ile Val Phe Gly Pro Gly Lys Gly Val 305 310 315 320 Gly Val Tyr Glu Ala Pro Arg Gly Thr Leu Ile His Leu Ile Glu Leu                 325 330 335 Gly Asp Glu Gly Arg Val Val Ser Ser Lys Ile Ile Val Pro Thr Met             340 345 350 Phe Asn Ile Pro Val Met Glu Glu Met Ala Lys Gly Leu Ser Val Lys         355 360 365 Ala Ala Glu Ala Val Met Arg Leu Tyr Asp Pro Cys Ile Pro Cys Thr     370 375 380 Thr His Val Val Arg Leu Gly Gly 385 390 <210> 77 <211> 225 <212> PRT <213> Thermococcus onnurineus NA1 <400> 77 Met Glu Lys Leu Lys Val Leu His Val Asp Val Gly Gly Cys Glu Gly   1 5 10 15 Cys Asn Val Ser Ile Ile Arg Ala Tyr Pro Lys Leu Met Asp Leu Ile              20 25 30 Glu Leu Asp Ile Ser Tyr Leu Arg Lys Asp Glu Cys Lys Leu Asp Glu          35 40 45 Tyr Asp Val Ala Ile Ile Thr Gly Gly Ala Cys Met Asn Glu Pro Arg      50 55 60 Ile Leu Glu Glu Leu Lys Glu Ile Arg Glu Lys Ala His Thr Val Val  65 70 75 80 Ala Phe Gly Ser Cys Ala Thr Phe Ser Gly Ile Leu Arg Phe Cys Arg                  85 90 95 Gly Gly Gln Glu Pro Arg Pro Asp His Arg Asn Phe Gln Pro Ile Asn             100 105 110 Ser Val Ile Lys Val Asp Tyr Ser Ile Pro Gly Cys Pro Pro Thr Pro         115 120 125 Gln Met Leu Gln Ser Phe Phe Lys Phe Tyr Ile Asn Gly Asp Glu Arg     130 135 140 Arg Leu Arg Leu Phe Lys Val Ser Ala Asp Ile Lys Lys Leu Ser Gly 145 150 155 160 Phe Asp Leu Ile Asp Asp Ile Val Leu Thr Gly Leu Cys Ile Gly Cys                 165 170 175 Gly Ala Cys Glu Leu Ser Cys Pro Thr Asn Ala Ile Lys Leu Ile Asp             180 185 190 Lys Arg Pro Asn Leu Val Gln Glu Lys Cys Ile Arg Cys Gly Thr Cys         195 200 205 Tyr Ile Arg Cys Pro Arg Ala Ser Gln Ile Leu Ser Met Gly Gly Ala     210 215 220 Arg 225 <210> 78 <211> 276 <212> PRT <213> Thermococcus onnurineus NA1 <400> 78 Met Met Ser Val Ser Glu Asn Leu Leu Gly Asn Val Phe Gly Ile Tyr   1 5 10 15 Leu Ala Arg Ala Thr Asp Glu Glu Ile Leu Lys Arg Lys Val Ala Ser              20 25 30 Gly Gly Ala Val Thr Ala Leu Leu Ala Tyr Ala Leu Glu Lys Gly Leu          35 40 45 Ile Asp Gly Val Val Thr Ala Lys Arg Thr Glu Gly Leu Glu Gly Gln      50 55 60 Ala Val Val Ala Arg Thr Arg Glu Glu Leu Leu Glu Thr Ala Gly Asn  65 70 75 80 Lys Trp Ser Ile Val Pro Phe Ala Ser Arg Met Lys Ala Lys Ile Glu                  85 90 95 Glu Glu Asp Leu Lys Asn Val Ala Val Val Cys Leu Pro Cys Gln Ala             100 105 110 Gln Phe Phe Gly Gln Met Arg Asp Phe Pro Leu Leu Glu Ser Asp Phe         115 120 125 Gly Glu Arg Ile Lys Tyr Ile Val Ser Leu Phe Cys Ile Gly Thr Phe     130 135 140 Ala Phe Glu Ala Phe Leu Asn Tyr Leu Arg Met Lys His Gly Ile Met 145 150 155 160 Ala Gln Asp Ile Lys Asp Ile Val Leu Lys Gly Asp Phe Leu Glu Ile                 165 170 175 Tyr His Gly Asp Ser Val Leu Ser Leu Pro Ile Lys Glu Val Tyr Ser             180 185 190 Tyr Leu Gln Ala Gly Cys Leu Val Cys Thr Asp Tyr Thr Gly Thr Trp         195 200 205 Ser Asp Ile Ser Ala Gly Phe Val Glu Ser Glu Arg Gly Trp Thr Val     210 215 220 Leu Ile Thr Arg Asn Leu Lys Ala Glu Glu Leu Val Lys Ser Ala Glu 225 230 235 240 Lys Asp Gly Tyr Ile Glu Leu Arg Asp Gly Ser His Val Met Gly Glu                 245 250 255 Val Leu Lys Ala Ala Arg Glu Lys Leu Ala Arg Ala Gln Lys Asn Met             260 265 270 Met tyr leu leu         275 <210> 79 <211> 252 <212> PRT <213> Thermococcus onnurineus NA1 <400> 79 Leu Ile Lys Lys Val Lys Ile Leu Lys Trp Gln Asp Gly Leu Val Pro   1 5 10 15 Thr Glu Asp Tyr Ile Cys Val Glu Glu Thr Phe Glu Ile Phe Ala Val              20 25 30 His Glu Lys Asp Glu Glu Phe Leu Ala Glu Leu Pro Ala Ser Pro Asn          35 40 45 Gln Leu Lys Glu Leu Gly Ala Gly Phe Val Val Cys Gly Gly Tyr Glu      50 55 60 Arg Pro Glu Asp Ile Val Asp Val Trp Val Glu Gly Lys Glu Ile Tyr  65 70 75 80 Val Lys Leu Lys Asp Thr Pro Ala Thr Gly Glu Leu Val Val Lys His                  85 90 95 Thr Pro Cys Gly Asp Pro Tyr Arg Met Lys Glu Gly Arg Ile Leu Ser             100 105 110 Arg Lys Gly Glu Glu Val Lys Ile Thr Pro Gly Leu Val Leu Lys Ile         115 120 125 Ser Ser Thr Met Thr Thr Leu Ala Glu Thr Trp Arg Lys Thr Gly Gly     130 135 140 Thr His Trp Ala Ala Leu Phe Asp Leu Asn Ala Asn Val Val Ala Phe 145 150 155 160 Ser Glu Asp Ile Gly Arg His Asn Ala Val Asp Lys Val Val Gly Tyr                 165 170 175 Ala Val Leu Asn Gly Leu Asp Leu Glu Arg Leu Ile Leu Ala Ser Ser             180 185 190 Gly Arg Met Pro Tyr Gly Met Val Arg Lys Ala Val Asn Ala Gly Ile         195 200 205 Pro Val Val Val Thr Lys Ser Pro Pro Thr Asp Lys Gly Val Glu Leu     210 215 220 Ala Arg Glu His Gly Val Thr Leu Ile Gly Phe Ala Arg Gly Arg Arg 225 230 235 240 Phe Asn Val Tyr Ser Gly Glu His Arg Leu Leu Phe                 245 250 <210> 80 <211> 711 <212> PRT <213> Thermococcus onnurineus NA1 <400> 80 Met Ser Glu Arg Leu Val Pro Val Val Cys Pro Tyr Cys Gly Val Gly   1 5 10 15 Cys Arg Leu Tyr Ile Arg Ser Val Asp Gly Tyr Pro Val Gly Ile Glu              20 25 30 Tyr Ala Lys Asp Ile Pro Asn Ile Ser Asn Glu Leu Gly Lys Leu Cys          35 40 45 Pro Lys Gly Asn Ala Val Val Glu Tyr Leu Leu Ala Lys Asp Arg Leu      50 55 60 Lys Arg Pro Leu Lys Ala Lys Glu Gln Gly Lys Phe Val Glu Ile Ser  65 70 75 80 Trp Ser Glu Ala Ile Lys Glu Val Ala Glu Arg Leu Lys Ala Tyr Ala                  85 90 95 Lys Asp Asp Pro Asn Gln Leu Met Phe Phe Gly Ser Ala Arg Thr Phe             100 105 110 Asn Glu Pro Asn Tyr Leu Val Gln Lys Leu Ala Arg Met Leu Gly Thr         115 120 125 Asn Asn Val Asp His Cys Ala Arg Leu Cys His Ala Pro Thr Val Thr     130 135 140 Gly Leu Lys Ala Val Phe Gly Ala Gly Ala Met Thr Asn Thr Tyr Lys 145 150 155 160 Asp Ile Glu Glu Ala Asn Val Ile Phe Ile Ile Gly His Asn Tyr Ala                 165 170 175 Glu Thr His Pro Val Gly Phe Arg Tyr Val Leu Lys Ala Lys Glu Arg             180 185 190 Gly Ala Lys Val Ile Val Ala Asp Pro Arg Phe Thr Arg Thr Ala Trp         195 200 205 Phe Ala Asp Ile Phe Leu Gln His Tyr Pro Gly Ser Asp Ile Ala Leu     210 215 220 Ile Asn Gly Leu Ile His Val Ile Ile Lys Glu Arg Leu Tyr Asp Glu 225 230 235 240 Lys Phe Val Arg Glu Arg Cys Val Gly Phe Asp Glu Val Val Ala Ala                 245 250 255 Val Glu Lys Phe Thr Pro Glu Phe Val Glu Lys Val Thr Gly Val Pro             260 265 270 Ala Glu Leu Ile Ile Glu Ala Ala Arg Thr Phe Ala Thr Ala Gly Lys         275 280 285 Gly Val Ile Thr Trp Ala Met Gly Leu Thr Gln His Thr His Gly Thr     290 295 300 Glu Asn Val Lys Leu Leu Gly Thr Leu Ala Ala Ile Cys Gly Tyr Gln 305 310 315 320 Gly Lys Glu Gly Ala Gly Cys Ser Pro Met Arg Gly Gln Asn Asn Val                 325 330 335 Gln Gly Ala Cys Asp Met Ala Ala Leu Pro Asn Val Phe Pro Gly Tyr             340 345 350 Gln Ala Val Thr Asp Pro Glu Lys Arg Lys Phe Phe Glu Glu Phe Trp         355 360 365 Gly Val Glu Leu Ser Gly Glu Val Gly Leu Thr Thr Val Glu Ala Ala     370 375 380 Tyr Ala Ala Asp Lys Gly Lys Val Lys Ala Tyr Tyr Val Met Gly Glu 385 390 395 400 Asn Pro Val Ile Ser Glu Ala Asn Ala Asn His Val Met His Thr Leu                 405 410 415 Glu Lys Leu Glu Phe Met Val Val Gln Asp Ile Val Pro Thr Pro Thr             420 425 430 Met Glu Tyr Ala Asp Ile Val Leu Pro Ala Ala Ala Met Leu Glu Asn         435 440 445 Glu Gly Ser Leu Thr Asn Thr Glu Arg Arg Val Gln Trp Ser Phe Gln     450 455 460 Ala Val Lys Pro Pro Gly Glu Ala Arg Pro Asp Trp Trp Ile Leu Ser 465 470 475 480 Glu Val Gly Lys Ala Ile Gly Phe Asp Lys Thr Gly Ser Gly Gly Phe                 485 490 495 Val Tyr Asn Asp Ala Ala Asp Val Leu Arg Glu Ile Asn Ala Cys Thr             500 505 510 Pro Gln Tyr Arg Gly Ile Thr Pro Glu Arg Leu Lys Glu Asn Leu Ala         515 520 525 Gly Leu His Trp Pro Cys Pro Ser Glu Asp His Pro Gly Thr Arg Val     530 535 540 Leu Tyr Lys Glu Lys Phe Leu Thr Pro Ser Gly Lys Ala Asn Leu Ala 545 550 555 560 Ala Val Pro Glu Tyr Lys Gly Pro Val Glu Met Pro Asp Glu Glu Tyr                 565 570 575 Pro Phe Leu Leu Thr Thr His Arg Tyr Val Gly Met Tyr His Thr Ala             580 585 590 Thr Met Thr Met Arg Ser Cys Ala Leu Lys Lys Arg Trp Pro Glu Pro         595 600 605 Leu Ala Glu Ile His Pro Asp Asp Ala Val Lys Leu Gly Ile Lys Ser     610 615 620 Gly Asp Trp Val Lys Val Val Thr Arg Arg Gly Ala Tyr Pro Ile Lys 625 630 635 640 Ala Lys Val Thr Arg Ala Val Lys Lys Gly Val Ile Ala Val Pro Trp                 645 650 655 His Trp Gly Ala Asn Val Leu Thr Asn Asp Ala Leu Asp Pro Val Ala             660 665 670 Lys Ile Pro Glu Thr Lys Ala Cys Ala Cys Asn Val Ala Lys Ile Thr         675 680 685 Glu Glu Glu Ala Arg Lys Leu Met Glu Lys Leu Pro Pro Leu Ile Pro     690 695 700 Lys Ile Glu Val Val Arg Gly 705 710 <210> 81 <211> 166 <212> PRT <213> Thermococcus onnurineus NA1 <400> 81 Met Ala Arg Lys Thr Val Phe Ile Asp Phe Ser Lys Cys Ile Glu Cys   1 5 10 15 Arg Ala Cys Glu Val Ala Cys Glu Arg Glu His Ser Gly Met Ser Phe              20 25 30 Ile Ser Val Phe Glu Trp Gln Glu Met Ala Ala Met Ala Leu Asn Cys          35 40 45 Arg His Cys Glu Lys Ala Pro Cys Val Glu Val Cys Pro Thr Asn Ala      50 55 60 Leu Tyr Arg Asp Lys Asp Gly Ala Val Leu Leu Ala Pro Gln Lys Cys  65 70 75 80 Ile Gly Cys Leu Met Cys Gly Ile Val Cys Pro Phe Gly Ile Pro Glu                  85 90 95 Leu Asp Leu Ile Asn Lys Ile Met Gly Lys Cys Asp Leu Cys Ala His             100 105 110 Arg Arg Ala Glu Gly Lys Leu Pro Ala Cys Val Glu Thr Cys Pro Thr         115 120 125 Asp Ala Leu Ile Tyr Gly Asp Phe Asn Glu Ile Val Lys Lys Arg Arg     130 135 140 Glu Lys Phe Thr Glu Lys Thr Ile Glu Leu Ala Lys Thr Ala Glu Arg 145 150 155 160 Ile Pro Leu Thr Gly Val                 165 <210> 82 <211> 480 <212> PRT <213> Thermococcus onnurineus NA1 <400> 82 Met Glu Glu Leu Phe Ile Leu Ser Phe Ser Ile Pro Leu Val Gly Gly   1 5 10 15 Leu Leu Leu Phe Lys Leu Asp Gly Lys Arg Ala Asp Tyr Phe Met Leu              20 25 30 Ile Thr Val Ile Leu Ala Thr Ile Leu Asn Leu Ala Gly Val Tyr Glu          35 40 45 Phe Tyr Ser Ser Gly Met Pro Thr Ile His Lys Thr Leu Val Ser Ser      50 55 60 Thr Thr Leu Gly Glu Val Tyr Gly Leu Leu Ile Asp Pro Met Ser Val  65 70 75 80 Cys Val Gly Leu Val Val Ile Thr Ala Gly Leu Leu Phe Met Ile Tyr                  85 90 95 Ala Lys Asp Tyr Met Ser Pro Glu Asn Lys Glu His Pro Val Tyr Glu             100 105 110 Asp Lys Gly Arg Phe Tyr Ala Trp Met Val Leu Phe Ile Gly Ala Thr         115 120 125 Leu Ala Phe Ile Tyr Ser Ser Ser Val Leu Gln Leu Leu Ile Phe Phe     130 135 140 Glu Ile Met Ser Leu Ala Cys Trp Gly Val Ala Gly Tyr Tyr Gly Ser 145 150 155 160 Lys Lys Ala Lys Arg Ala Ala Tyr Lys Ala Leu Leu Val Thr Asn Phe                 165 170 175 Gly Ala Val Ile Gly Leu Tyr Thr Ala Val Gly Ile Gly Ile Thr His             180 185 190 Leu His Asp Leu Ser Ile Phe Ala Tyr Ser Gly Leu Asn Asp Ser Leu         195 200 205 Lys Leu Val Val Phe Ile Gly Val Met Ile Ala Ala Phe Thr Lys Ser     210 215 220 Ala Gln Phe Pro Leu Tyr Ser Trp Leu Pro Asp Ala Met Val Ala Pro 225 230 235 240 Thr Pro Ala Ser Ala Phe Leu His Gly Ala Ala Met Val Glu Met Gly                 245 250 255 Val Tyr Leu Leu Ala Arg Phe Ile Gln Phe Met Asn Pro Ile Pro Lys             260 265 270 Glu Gly Phe Tyr Val Met Ala Ala Leu Ile Ile Ala Thr Gln Ile Ile         275 280 285 Cys Ile Leu Met Tyr Pro Leu Gln Lys Ser Ala Lys Arg Leu Leu Ala     290 295 300 Tyr Ser Thr Ile Ala Glu Ser Gly Leu Met Tyr Val Ala Leu Ala Thr 305 310 315 320 Ala Val Leu Gly Leu Gln Gly Gly Leu Gln Ala Ser Met Phe Gln Leu                 325 330 335 Phe Asn His Ala Tyr Ile Lys Gly Leu Ala Phe Leu Thr Ala Gly Thr             340 345 350 Phe Ser Tyr Ala Leu Gly Thr Leu Glu Met Asp Arg Ile Lys Gly Leu         355 360 365 Ile Lys Ser Pro Val Val Gly Tyr Ser Trp Thr Phe Ala Leu Leu Gly     370 375 380 Leu Ala Gly Val Pro Pro Phe Gly Val Phe Phe Gly Lys Leu Gly Ile 385 390 395 400 Leu Ser Asn Ala Lys Ala Met Glu Glu Ser Val Leu Ile Ila Ala Met                 405 410 415 Phe Val Leu Leu Leu Leu Asp Ser Ala Val Phe Leu Met Val Ser Leu             420 425 430 Lys Arg Ile His Gly Met Val Phe Ser Glu Gly Gly Glu Glu Val Glu         435 440 445 Ile Thr Pro Leu Met Lys Ala Val Met Val Ile Leu Leu Val Leu Ala     450 455 460 Met Leu Ala Pro Tyr Ile Ala Tyr Pro Leu Ile Val Lys Val Gly Trp 465 470 475 480 <210> 83 <211> 404 <212> PRT <213> Thermococcus onnurineus NA1 <400> 83 Met Phe Asp Val Thr Leu Thr Leu Ser Leu Asp Arg Thr Ala Val Phe   1 5 10 15 Phe Val Leu Asn Val Ala Ile Leu Gly Ile Ala Ala Leu Val Ala Ser              20 25 30 Phe Arg Tyr Met Arg Ile Tyr Glu Phe Lys Pro Lys Ile Pro Tyr Tyr          35 40 45 Pro Thr Leu Ala Ile Phe Ile Val Ser Met Leu Leu Ile Pro Met Val      50 55 60 Gln Asp Trp Leu Ser Phe Leu Phe Leu Trp Glu Ile Met Thr Leu Ala  65 70 75 80 Ser Tyr Phe Leu Ile Ile Tyr Asp Trp Pro Glu Glu Ser Val Lys Lys                  85 90 95 Ala Gly Trp Lys Tyr Phe Val Thr Met His Leu Phe Asp Thr Ser Pro             100 105 110 Leu Met Leu Ala Val Thr Met Tyr Tyr Ala Phe His Gly Thr Phe Asn         115 120 125 Phe Gly Ala Ile Thr Glu Tyr Ser Asn Ala Ile Val Ala Leu Phe Leu     130 135 140 Leu Gly Phe Ala Ala Lys Ala Gly Leu Phe Pro Leu His Phe Trp Leu 145 150 155 160 Pro Asp Ala His Pro Ala Ala Pro Ser Pro Val Ser Ala Leu Met Ser                 165 170 175 Gly Ala Met Val Glu Leu Gly Leu Tyr Gly Thr Ile Arg Val Leu Asn             180 185 190 Ala Val Gly Trp Ser Val Ala Thr Trp Ile Val Tyr Leu Ile Gly Ala         195 200 205 Met Ala Val Leu Ser Met Leu Ala Ala Ile Phe Ser Tyr Ala Leu Gln     210 215 220 Asp Asp Val Lys Arg Leu Phe Ala Trp Ser Thr Ile Asp Asn Met Gly 225 230 235 240 Trp Met Tyr Leu Leu Ile Leu Ala Gly Leu Leu Gly Val Ser Gly Val                 245 250 255 Glu Lys Gly Val Asp Tyr Tyr Val Val Ala His Gly Leu Ala Lys Ala             260 265 270 Ala Ala Phe Ile Ser Thr Gly Ala Leu Leu Tyr Val Phe Gly Thr Arg         275 280 285 Ser Leu Lys Lys Ala Lys Gly Met Met Asn Thr Asp Ser Leu Thr Ala     290 295 300 Gly Leu Met Met Ala Ser Ile Phe Ala Leu Glu Gly Val Pro Pro Phe 305 310 315 320 Asn Leu Phe Met Asn Lys Leu Asn Val Ile Lys Thr Leu Leu Thr Val                 325 330 335 Ser Pro Ala Leu Ala Tyr Phe Thr Ala Leu Glu Trp Val Ile Ala Phe             340 345 350 Ile Leu Phe Leu Arg Val Val His Ala Tyr Ile Leu Ser Glu Gly Glu         355 360 365 Pro Glu Ala Lys Arg Lys Leu Ala Gly Ser Ile Ala Leu Ser Val Ile     370 375 380 Val Leu Leu Ile Leu Ser Met Val Ser Gln Phe Val Cys Asp Tyr Ile 385 390 395 400 Trp Val Arg Trp                 <210> 84 <211> 690 <212> PRT <213> Thermococcus onnurineus NA1 <400> 84 Met Glu Gly Leu Phe Thr Leu Ala Val Ile Leu Tyr Phe Leu Ser Ile   1 5 10 15 Pro Ala Ala Leu Ala Leu Lys Arg Ser Phe Lys Ala Ser Ile Ser Ile              20 25 30 Gly His Ile Leu Thr Ala Leu Ala Ser Ile Ala Leu Leu Ala Phe Thr          35 40 45 Phe Val Ser Ile Pro Asp Ile Leu Ser Gly Lys Ala Ile Glu Phe Thr      50 55 60 Tyr Asp Leu Gly Val Ala Gln Ile Pro Phe Gln Ile Asp Gly Leu Ser  65 70 75 80 Leu Ile Met Cys Phe Ile Phe Gly Ala Leu Gly Leu Ala Ala Ser Ile                  85 90 95 Tyr Ser Pro Arg Tyr Met Ala Ile Tyr Glu Lys Ser Gly Arg Gly Trp             100 105 110 Met Tyr Ile Thr Ile Tyr Ser Val Phe Met Leu Ser Met Ile Leu Ile         115 120 125 Val Thr Ile Ala Asn Met Phe Trp Phe Ile Phe Leu Trp Glu Val Met     130 135 140 Thr Phe Thr Ser Tyr Leu Leu Thr Ile Trp Glu Ser Asp Lys Glu Asp 145 150 155 160 Val Arg Lys Ala Gly Trp Lys Tyr Phe Val Thr Met His Ile Val Ser                 165 170 175 Thr Leu Pro Leu Ile Ile Ala Leu Ala Leu Leu Tyr Ala Asp Val Ser             180 185 190 Ser Ile Glu Gly Leu Asn Phe Glu Ser Leu Ala Ala Leu Lys Leu Ser         195 200 205 Pro Val Phe Tyr Ala Leu Phe Leu Ile Gly Phe Gly Ser Lys Ser Gly     210 215 220 Val Val Pro Leu His Phe Trp Ala Pro Glu Ala Tyr Thr Val Ala Pro 225 230 235 240 Ser Asn Val Ser Ala Leu Met Ala Gly Ala Leu Glu Lys Val Ala Val                 245 250 255 Tyr Ala Leu Ile Arg Thr Thr Cys Phe Ile Met Lys Pro Asn Glu Thr             260 265 270 Phe Gly Tyr Ala Val Ala Leu Leu Gly Thr Val Thr Leu Thr Val Gly         275 280 285 Thr Leu Tyr Ala Leu Lys Gln Thr Asp Ala Lys Arg Leu Leu Ala Tyr     290 295 300 His Ser Ile Gly Gln Ile Gly Tyr Ile Trp Leu Gly Met Gly Val Gly 305 310 315 320 Ile Val Phe Ile Ala Arg Gly Asp Met Tyr Ser Ala Phe Gly Ala Ile                 325 330 335 Ala Leu Ala Ser Ser Le Le Tyr His Leu Val Asn His Thr Phe Phe Lys             340 345 350 Gly Leu Leu Phe Leu Ser Thr Gly Ser Ile Phe Tyr Arg Thr Arg Ser         355 360 365 Arg Asp Leu Asn Gln Leu Arg Gly Leu Ala Lys Leu Met Pro Phe Thr     370 375 380 Ala Leu Phe Thr Phe Ile Ala Ala Met Ser Ile Ala Gly Thr Pro Pro 385 390 395 400 Phe Asn Gly Phe Met Ser Lys Trp Met Ile Tyr Gln Ser Thr Phe Leu                 405 410 415 Ser Gly Asn Gly Leu Ile Val Phe Phe Gly Val Met Ala Leu Phe Ile             420 425 430 Ser Ala Ala Thr Leu Ala Ser Phe Ile Lys Phe Tyr Thr Thr Ala Phe         435 440 445 Gly Gly Glu Pro Thr Glu Phe Thr Lys Asp Ala Glu Glu Val Pro Ser     450 455 460 Pro Met Leu Ile Ala Lys Gly Phe Leu Ala Ser Leu Cys Ile Leu Leu 465 470 475 480 Gly Leu Val Pro Ser Leu Ile Leu Pro Ile Leu Leu Ser Pro Gly Ala                 485 490 495 Ala Leu Ala Gly Ile Asp Val Ser Gly Leu Met Asp Thr Asn Tyr Trp             500 505 510 Leu Val Thr Ile Lys Ala Pro Leu Met Pro Thr Gly Ala Glu Ser Tyr         515 520 525 Phe Lys Pro Leu Leu Phe Ala Thr Leu Phe Gly Val Ile Phe Leu Gly     530 535 540 Met Tyr Leu Leu Phe Pro Ile Ser Lys Lys Thr Tyr Arg Pro Trp Thr 545 550 555 560 Leu Gly Glu Pro Val Ala Met Glu His Tyr Lys Phe Lys Ala Ile Asn                 565 570 575 Tyr Tyr Glu Pro Phe Glu Glu Tyr Ile His Pro Leu Tyr His Thr Gly             580 585 590 His Val Leu Ser Glu Phe Gly Ser Ala Leu Ile Gly Ala Val Ala Asn         595 600 605 Ala Tyr Val Ser Thr Thr Arg Ala Leu His Arg Val Cys Asp Ser Ile     610 615 620 Ser Lys Ser Val Ala Gly Ile Gly Lys Glu Tyr Glu Lys Lys Cys Pro 625 630 635 640 Glu Val Tyr Leu Asp Glu Tyr Phe Leu Ala Pro Leu Val Lys Ile Val                 645 650 655 Arg Val Ser Gly Val Leu Leu Asp Glu Gly Phe Met Arg Pro Asn Ala             660 665 670 Ala Phe Thr Ile Ala Leu Val Thr Leu Ala Val Ile Leu Ala Leu Met         675 680 685 Val leu     690 <210> 85 <211> 307 <212> PRT <213> Thermococcus onnurineus NA1 <400> 85 Met Thr Leu Glu Lys Ile Ala Phe Ala Ala Leu Ser Leu Met Ile Ile   1 5 10 15 Ile Leu Leu Pro Pro Leu Leu Asp Gly Ile Ser Arg Lys Ile Lys Ala              20 25 30 Thr Val Gln Glu Arg Gln Gly Pro Pro Val Phe Gln Thr Tyr Tyr Asp          35 40 45 Leu Ser Ser Leu Leu Ser Met Glu Pro Ile Leu Pro Thr Asp Arg Leu      50 55 60 Gly Phe Leu Ile Ala Pro Tyr Val Ala Phe Ala Ser Ala Val Ser Ala  65 70 75 80 Ala Leu Leu Leu Pro Phe Gly Asn Phe Val Pro Val Ala Phe Thr Gly                  85 90 95 Asp Ile Phe Val Phe Leu Tyr Val Leu Ala Ile Phe Ser Ile Ser Met             100 105 110 Met Met Ala Gly Phe Leu Val Asn Asn Thr Tyr Ser Asn Ala Gly Ala         115 120 125 Asn Arg Glu Met Met Leu Ile Leu Ser Val Glu Pro Ile Leu Gly Ile     130 135 140 Ala Ile Gly Ile Leu Ala Leu Lys Thr His Ser Leu Ser Val Ser Gly 145 150 155 160 Ile Pro Leu Asn Leu Ser Leu Thr Pro Ser Val Val Leu Ala Phe Ile                 165 170 175 Phe Leu Ala Tyr Ala Val Tyr Thr Glu Cys Ala Phe Ile Pro Phe Asp             180 185 190 Ile Ala Glu Ala Glu Thr Glu Ile Leu Glu Gly Pro Leu Val Glu Tyr         195 200 205 Ser Gly Lys Leu Leu Gly Ile Phe Lys Trp Ala Met Leu Ile Lys Arg     210 215 220 Val Ala Leu Ile Trp Leu Phe Ala Ser Phe Ile Val Ile Pro Val Met 225 230 235 240 Lys Gly Phe Val Asp Ile Thr Thr Pro Tyr Gly Gly Ala Val Thr Leu                 245 250 255 Ala Ala Gln Leu Val Leu Leu Val Val Phe Tyr Val Met Ser Ala Ile             260 265 270 Ile Glu Ser Thr Thr Ala Arg Met Lys Val Ile Gln Ala Ile Arg Gln         275 280 285 Asn Thr Val Ile Phe Leu Ala Gly Ile Val Ala Leu Val Ile Ala Ser     290 295 300 Leu Gly Trp 305 <210> 86 <211> 584 <212> PRT <213> Thermococcus onnurineus NA1 <400> 86 Met Ser Glu Val Ile Lys Phe Asn Glu Ala Leu Lys Lys Lys Arg Val   1 5 10 15 His Arg Gly Asp Glu Lys Ala Lys Val Thr Arg Glu Tyr Leu Asp Glu              20 25 30 Ile Ile Glu Lys Phe Gly Glu Lys Ile Arg Asp Val Lys Gln Ala Ala          35 40 45 Tyr Asn Gln Trp Ile Ile Thr Val Glu Arg Glu Asp Leu Pro Glu Ile      50 55 60 Val Leu Tyr Phe Leu Asn His Pro Glu Trp Lys Glu Thr Gln Leu Ser  65 70 75 80 Ser Met Val Ala Thr Asp Glu Arg Pro Leu Asn Gly Lys Phe Ser Ile                  85 90 95 Thr Tyr Trp Leu Ser Val Asn Gly Lys Ala Gly Asp Phe Tyr Leu Gly             100 105 110 Val Arg Ala Tyr Leu Pro Glu Asp Asp Pro Arg Phe Thr Ser Ile Ala         115 120 125 Ala Lys His Arg Gly Ala Asn Trp Tyr Glu Arg Glu Ala Met Glu Met     130 135 140 Leu Gly Leu Thr Ala Glu Gly His Pro Asp Pro Arg Arg Leu Val Leu 145 150 155 160 Pro Asp Asp Trp Pro Ser Cys Val Tyr Pro Leu Arg Lys Asp Phe His                 165 170 175 Tyr Ser Asn Ser Pro Pro Gly Glu Lys Phe Tyr Pro Tyr Lys Glu Pro             180 185 190 Lys Lys Asp Glu Ile Val Val Pro Tyr Gly Pro Tyr His Val Ala Leu         195 200 205 Glu Glu Ala Ala His Phe Arg Leu Tyr Val Lys Gly Glu Thr Ile Thr     210 215 220 Asp Val Asp Tyr Arg Gly Phe Tyr Ala His Arg Gly Ile Glu Lys Ile 225 230 235 240 Ser Glu Gly Arg Leu Thr Tyr Asp Gln Val Cys Phe Ile Ala Glu Arg                 245 250 255 Ile Cys Gly Ile Cys Gly Cys Thr His Ser Thr Ala Tyr Cys Gln Ala             260 265 270 Val Glu Asn Ala Gly Gly Ile Glu Val Pro Glu Arg Ala Glu Tyr Ile         275 280 285 Arg Thr Ile Val Leu Glu Ile Glu Arg Leu His Ser His Leu Leu Asn     290 295 300 Phe Gly Ile Val Ser His Leu Val Gly Tyr Asp Tyr Gly Phe Met Lys 305 310 315 320 Ala Trp Arg Ile Arg Glu His Val Met Trp Leu Ala Glu Arg Leu Thr                 325 330 335 Gly Asn Arg Lys Thr Tyr Gly Met Leu Leu Val Gly Gly Val Arg Arg             340 345 350 Asp Leu Leu Glu Tyr Arg Lys Ser Leu Ile Glu Asp Val Leu Lys Lys         355 360 365 Ile Lys Thr Glu Phe Ser Glu Leu Val Asp Glu Ala Ile Ser Thr Ser     370 375 380 Thr Phe Val Lys Arg Leu Glu Gly Val Gly Val Leu Pro Tyr Lys Val 385 390 395 400 Ala Lys Glu Trp Asp Val Asp Gly Pro Leu Gly Arg Gly Ser Gly Arg                 405 410 415 Asp Phe Asp Val Arg Arg Asp His Pro Tyr Ala Ala Tyr Lys Tyr Leu             420 425 430 Asp Phe Lys Val Pro Val Tyr Lys Glu Gly Asp Val Leu Ala Arg Ala         435 440 445 Leu Val Arg Ile Glu Glu Val Phe Glu Ser Ile Trp Ile Ile Glu Gln     450 455 460 Ala Leu Asp Gln Met Pro Gly Gly Asp Ile Leu Ala Glu Tyr Lys Glu 465 470 475 480 Ile Pro Pro Tyr Ser Glu Ala Ile Gly Met Thr Glu Ala Pro Arg Gly                 485 490 495 Glu Asn Ile His Tyr Val Met Thr Gly Glu Asn Asn Lys Val Tyr Arg             500 505 510 Tyr Arg Ala Arg Ala Ala Thr Tyr Asn Asn Leu Pro Ala Val Pro Asp         515 520 525 Met Met Arg Gly Tyr Thr Ile Ala Asp Ala Pro Leu Ile Val Ala Ser     530 535 540 Ile Asp Pro Cys Tyr Ser Cys Thr Glu Arg Val Gln Val Val Asp Val 545 550 555 560 Glu Ser Gly Lys Val Arg Val Leu Ser Glu Thr Glu Phe Asn Lys Leu                 565 570 575 Ser Ile Lys Ala Ser Arg Arg Val             580 <210> 87 <211> 165 <212> PRT <213> Thermococcus onnurineus NA1 <400> 87 Met Ala Val Thr Leu Lys Tyr Pro Phe Val Lys Leu Glu Ala Pro Pro   1 5 10 15 Glu Tyr Arg Gly Ile Pro Gln Ile Asp Ala Thr Leu Cys Ile Gly Cys              20 25 30 Gly Ala Cys Val Asn Ala Cys Pro Pro Asp Ala Leu Leu Arg Ile Asp          35 40 45 Asp Tyr Asn Arg Gly Val Arg Glu Ile Val Leu Asp Val Gly Arg Cys      50 55 60 Ile Arg Cys Ala Arg Cys Glu Glu Val Cys Pro Thr Gly Ala Ile Lys  65 70 75 80 Leu Thr Asn Leu Phe Glu Ala Ala Ser Pro Asp Arg Met Asp His Val                  85 90 95 Glu Val Val Arg Leu Arg Leu Val Lys Cys Lys Asn Cys Gly Arg Tyr             100 105 110 Ala Asp Phe Thr Glu Arg Gln Val Arg Lys Ala Leu Gln Ile Leu Pro         115 120 125 Glu Glu Ile Ile Glu Arg Glu Ala Leu Glu Glu Lys Val Trp Ile Cys     130 135 140 Arg Asp Cys Arg Arg Lys Gly Thr Val Asp Gly Thr Ile Glu Ala Ser 145 150 155 160 Lys Glu Val Val Leu                 165 <210> 88 <211> 273 <212> PRT <213> Thermococcus onnurineus NA1 <400> 88 Met Ser Gly Lys Pro Lys Leu Arg Ser Ile Trp Val Phe His Leu Asn   1 5 10 15 Thr Gly Ser Cys Asn Gly Cys Asp Ile Glu Ile Ile Asp Val Leu Thr              20 25 30 Pro Phe Tyr Asp Val Glu Arg Phe Gly Ile Lys Leu Val Gly Ser Pro          35 40 45 Arg His Ala His Ala Leu Leu Val Ser Gly Pro Leu Thr Arg Gln Ala      50 55 60 Tyr Tyr Gly Ala Lys Glu Thr Ile Lys Ala Met Pro Pro Glu Pro Arg  65 70 75 80 Val Ile Val Ala Ile Gly Thr Cys Thr Cys Ser Gly Gly Ile Phe Tyr                  85 90 95 Asn Gly Tyr Pro Val Tyr Arg Arg Pro Glu Ser Gly Arg Glu Gly Ser             100 105 110 Glu Tyr Pro Arg Arg Gly Gly Ile Ala Glu Leu Ile Ala Asp Leu Arg         115 120 125 Asp Glu Gly Glu Lys Val Gly Pro Val Ile Tyr Ile Pro Gly Cys Pro     130 135 140 Pro Arg Pro Glu Glu Ile Ile Tyr Gly Ile Ala Gln Leu Val Gly Leu 145 150 155 160 Val Glu Lys Lys Leu Ser Tyr Gln Glu Tyr Ser Asp Glu Leu Val Pro                 165 170 175 Phe Lys Leu Pro Glu Gly Pro Leu Glu Glu Arg Ile Arg Leu Thr Leu             180 185 190 Met Glu Arg Leu Arg His Leu Val Gly Tyr Leu Asp Arg Glu Lys Ile         195 200 205 Leu Glu Asp Phe Met Gly Leu Val Lys Glu Ala Glu Lys Ser Glu Asn     210 215 220 Pro Arg Glu Glu Leu Ala Arg Leu Val Lys Asp Tyr Ala Ala Lys Cys 225 230 235 240 Gly Asp Val Arg Leu Gly Phe Cys Met Met Leu Leu Glu Arg Glu Tyr                 245 250 255 Trp Arg Val Lys Asp Ala Leu Asp Ala Gly Lys Glu Phe Val Tyr Trp             260 265 270 Val     <210> 89 <211> 93 <212> PRT <213> Thermococcus onnurineus NA1 <400> 89 Met Pro Trp Met Leu Val Lys Ser Ser Tyr Ile Gly Phe Lys Thr Tyr   1 5 10 15 Leu Ala Gly Ala Leu Ser His Thr Glu Gly Asp Phe Glu Val Glu Glu              20 25 30 Ile Val Gly Glu Ile Ser Pro Arg Ala Ala His Leu Leu Arg Lys Ser          35 40 45 Phe Glu Arg Ser Tyr Phe Thr Leu Ala Asp Ala Pro Leu Ile Pro Phe      50 55 60 Glu Glu Leu Asp Glu Gly Asp Arg Arg Leu Ile Leu Lys Ala Leu Arg  65 70 75 80 Gly Leu Arg Glu Asn Glu Arg Leu Lys Ile Glu Arg Arg                  85 90 <210> 90 <211> 342 <212> PRT <213> Thermococcus onnurineus NA1 <400> 90 Met Ala Glu Thr Lys Glu Lys Ile Leu Tyr Gly Val Asp Thr Thr Phe   1 5 10 15 Glu Ala Val Ala Lys Lys Ala Thr Pro Lys Phe Lys Thr Thr Pro Gly              20 25 30 Arg Leu Leu Phe Ala Gly Phe Met Ala Gly Ala Phe Ile Ala Phe Gly          35 40 45 Phe Leu Leu Ala Val Val Ala Ala Ala Gly Tyr Ser Pro Lys Leu Phe      50 55 60 Pro Asp Thr Gly Asn Ile Ser Thr Phe Lys Ile Leu Leu Gly Ala Val  65 70 75 80 Phe Pro Val Gly Leu Ile Ala Val Ile Leu Ala Gly Ala Asp Leu Trp                  85 90 95 Thr Gly Asn Val Gln Phe Leu Ser Ser Ala Lys Ala Lys Gly Tyr Ala             100 105 110 Asp Phe Lys Cys Val Leu Tyr Asn Trp Phe Gly Ser Tyr Gly Gly Asn         115 120 125 Phe Ile Gly Ser Ile Phe Leu Ala Leu Leu Ala Val Pro Leu Thr Gly     130 135 140 Leu Phe Gly His Val Gly Asp Pro Asn Thr Phe Gly Gln Val Thr Val 145 150 155 160 Gly Ile Ala Thr Gly Lys Val Ser Lys Asp Ile Leu Ala Leu Phe Phe                 165 170 175 Leu Gly Ile Gly Cys Asn Trp Leu Val Asn Val Ala Ile Trp Gln Ser             180 185 190 Ala Arg Val Gln Asp Gly Ala Gly Lys Ile Leu Ala Ile Trp Phe Pro         195 200 205 Ile Phe Ala Phe Val Ala Ile Gly Phe Glu His Ala Ile Ala Asn Met     210 215 220 Trp Ala Ile Pro Ala Gly Ile Leu Leu Ser Asp Tyr Ala Ile Thr Trp 225 230 235 240 Thr Gln Phe Phe His Asn Val Ile Pro Val Thr Phe Gly Asn Ala Ile                 245 250 255 Gly Gly Phe Leu Phe Val Thr Phe Tyr Tyr Trp Tyr Leu Ser His Pro             260 265 270 Glu Leu Thr Thr Asp Arg Leu Ile Lys Glu Ile Ile Asp Phe Leu Ile         275 280 285 Val Phe Ile Ala Phe Trp Ala Val Ala Ala Leu Ile Pro Ala Gly Ile     290 295 300 Gly Ile Ala Leu Asp Gln Ala Leu Gly Lys Gly Ala Met Tyr Leu Val 305 310 315 320 Pro Leu Val Leu Ser Ala Tyr Tyr Ile Val Gly Ala Phe Val Leu Tyr                 325 330 335 Lys Lys Ala Arg Pro Ala             340 <210> 91 <211> 93 <212> PRT <213> Thermococcus onnurineus NA1 <400> 91 Met Leu Glu Ser Thr Phe Met Thr Leu Met Lys Leu Ile Ile Pro Leu   1 5 10 15 Tyr Ile Ala Ala Phe Val Ile Tyr Ala Val Arg Ala Phe Lys Gly Pro              20 25 30 Ser Ile Pro Asp Val Ile Leu Ala Val Asp Cys Met Ser Phe Asp Ile          35 40 45 Ala Ala Phe Met Ala Ile Leu Ala Val Tyr Phe Lys Ser Thr Phe Leu      50 55 60 Val Ser Ala Ala Ile Ile Leu Ala Leu Trp Ala Tyr Leu Leu Asp Ile  65 70 75 80 Tyr Val Ala Lys Tyr Leu Ala Asn Gly Glu Val Gly Ala                  85 90 <210> 92 <211> 119 <212> PRT <213> Thermococcus onnurineus NA1 <400> 92 Met Ser Glu Ile Leu Phe Tyr Leu Gly Ala Val Met Ile Ile Ile Gly   1 5 10 15 Gly Ile Cys Asp Leu Phe Gly Ala Ile Gly Leu Leu Arg Phe Pro Asn              20 25 30 Phe Tyr Val Arg Leu His Ala Ala Thr Val Gly Thr Ile Gly Gly Ala          35 40 45 Val Val Pro Leu Phe Gly Val Ala Leu Leu Ala Leu Gly Ala Asp Phe      50 55 60 Leu Pro His Arg Tyr Ala Ile Ala Gly Ala Ser Phe Ile Thr Gly Ile  65 70 75 80 Ile Val Leu Leu Ala Ala Pro Ala Gly Ala Thr Ala Leu Ala Tyr Ala                  85 90 95 Ala His Lys Ala Arg Leu Val Glu Trp Lys Pro Lys Val Asp His Leu             100 105 110 Ala Glu Val Arg Gly Asp Asp         115 <210> 93 <211> 84 <212> PRT <213> Thermococcus onnurineus NA1 <400> 93 Met Ile Glu Ile His Leu Leu Ile Leu Ala Thr Val Thr Val Leu Gly   1 5 10 15 Met Val Phe Ser Tyr Leu Ala Ile Thr Glu Lys Asp Leu Leu Lys Ala              20 25 30 Val Gly Phe Ser Ala Val Gln Ala Ile Ala Tyr Ala Ile Ala Phe Tyr          35 40 45 Ile Leu Met Ala Pro Asp Ile Val Leu Ala Tyr Val Ala Ile Ala Val      50 55 60 Gly Ile Tyr Ser Ala Leu Leu Val Phe Val Ile Ser Lys Thr Glu Arg  65 70 75 80 Tyr Glu Val Val                 <210> 94 <211> 251 <212> PRT <213> Thermococcus onnurineus NA1 <400> 94 Met Lys Arg Trp Val Thr Leu Ile Leu Leu Leu Thr Ala Val Ile Thr   1 5 10 15 Leu Ala Tyr Val Phe Gln Val Pro Gln Pro Glu Asp Val Lys Pro Leu              20 25 30 Gly Glu Phe Tyr Leu Glu Asn Ser Tyr Phe Gly Asp Tyr Ser Ala Lys          35 40 45 Ser Pro Glu Val Ile Thr Ser Ile Leu Trp Asp Tyr Arg Gly Ile Asp      50 55 60 Thr Leu Phe Glu Thr Ala Val Phe Phe Leu Ala Ile Gly Ser Leu  65 70 75 80 Thr Val Phe Arg Leu Thr Lys Glu Gln Glu Lys Glu Val Lys Thr Glu                  85 90 95 Pro Thr Gln Val Glu Pro Leu Pro Leu Pro Ile Arg Thr Val Thr Lys             100 105 110 Val Ile Val Ala Met Ile Leu Ala Val Ser Ala Ser Ile Ala Leu His         115 120 125 Gly His Leu Thr Pro Gly Gly Gly Phe Gln Gly Gly Ser Ala Leu Ala     130 135 140 Val Ala Pro Leu Leu Ile Ile Ala Ala Tyr Ser Lys Tyr Ala Leu Glu 145 150 155 160 Lys Asn Gly Leu Asp Lys Thr Arg Ala Leu Ile Leu Arg Ser Ile Gly                 165 170 175 Leu Leu Gly Ile Ala Leu Val Ala Leu Val Pro Leu Leu Ser Gly Ser             180 185 190 Tyr Ile Met Gln Asn Gln Pro Val Phe Pro Ala Glu Ile Asn Gly Gln         195 200 205 Leu Ile Gly Gly Ser Leu Ile Tyr Tyr Asn Phe Phe Glu Phe Leu Ala     210 215 220 Val Gly Ala Gly Phe Thr Ala Val Phe Leu Leu Leu Ala Ile Pro Glu 225 230 235 240 Glu Lys Phe Lys Lys Ile Leu Gly Val Arg Arg                 245 250 <210> 95 <211> 134 <212> PRT <213> Thermococcus onnurineus NA1 <400> 95 Met Ile Gly Phe Leu Trp Ser Leu Val Val Ile Ser Leu Met Thr Thr   1 5 10 15 Leu Leu Ile Gly Leu Tyr Gly Ile Ala Arg Arg Pro Asn Leu Val Lys              20 25 30 Lys Leu Ile Ala Leu Thr Ile Ile Gly Asp Thr Ala Asn Leu Leu Val          35 40 45 Val Met Leu Gly Tyr Arg Leu Thr Tyr Pro Thr Ala Pro Pro Ile Leu      50 55 60 Pro Ser Leu Glu Lys Ser Ala Leu Gly Ser Phe Leu Ser Ala Ala Val  65 70 75 80 Asp Pro Leu Pro Gln Ala Leu Val Ile Thr Ala Val Val Ile Gly Met                  85 90 95 Ala Val Asn Val Leu Ile Ala Phe Ala Ile Val Gln Ile Tyr Arg Leu             100 105 110 Tyr Gly Thr Leu Asp Ala Arg Glu Ile Gly Val Lys Arg Ser Ser Pro         115 120 125 Leu Glu Gly Glu Lys Ile     130 <210> 96 <211> 161 <212> PRT <213> Thermococcus onnurineus NA1 <400> 96 Met Arg Gly Val Ile Pro Thr Ala Leu Leu Ala Phe Ile Thr Tyr Ile   1 5 10 15 Leu Phe Thr Gly Ser Ala Thr Pro Tyr Asp Leu Ala Thr Gly Ala Ile              20 25 30 Val Ala Ile Ala Val Gly Leu Leu Ala Gly Lys Phe Leu Val Lys Asn          35 40 45 Asp Ala Lys Ala Leu Asn Pro Ala Arg Trp Leu Trp Leu Gly Ala Tyr      50 55 60 Phe Leu Trp Tyr Met Leu Val Ala Glu Val Lys Ser His Ile Asp Val  65 70 75 80 Met Leu Arg Ile Ile Thr Gly Asn Val Lys Pro Gly Ile Val Lys Val                  85 90 95 Pro Ile Asp Val Lys Thr Glu Tyr Ala Lys Thr Leu Val Ala Asn Ser             100 105 110 Ile Thr Asn Thr Pro Gly Thr Val Val Val Asp Met Asp Glu Asn Tyr         115 120 125 Ile Tyr Val Asn Trp Ile Asp Thr Ser Thr Glu Asp Pro Glu Glu Ala     130 135 140 Arg Ser Glu Ile Ser Ala Asp Phe Glu Lys Tyr Ala Lys Lys Ile Phe 145 150 155 160 Glu     <210> 97 <211> 522 <212> PRT <213> Thermococcus onnurineus NA1 <400> 97 Met Asp Ile Val Gly Leu Thr Pro Val Ile Pro Ile Leu Phe Ala Phe   1 5 10 15 Ala Leu Pro Leu Thr Ser Ile Ile Val Arg Gly Asn Arg Lys Ile Ile              20 25 30 Gln Ala Tyr Ala Leu Ile Gly Thr Gly Leu Thr Leu Leu Ala Ser Tyr          35 40 45 Glu Leu Phe Arg Gln Val Tyr Asn Ser Ser Lys Pro Leu Ile Tyr Thr      50 55 60 Phe Gly Gly Trp Thr Ala Pro Val Gly Ile Ile Tyr Glu Val Asp Arg  65 70 75 80 Met Ser Ala Leu Phe Ala Leu Val Thr Ala Ala Leu Met Phe Leu Ile                  85 90 95 Ala Ile Tyr Ser Tyr Arg Tyr Leu Glu His Glu Glu Gly Leu Glu Trp             100 105 110 Tyr Tyr Thr Leu Tyr Leu Gly Leu Glu Ala Gly Leu Leu Gly Val Leu         115 120 125 Leu Thr Gly Asp Ala Phe Asn Leu Phe Val Met Ile Glu Val Thr Ser     130 135 140 Ile Ala Ala Tyr Ala Leu Val Met Phe Tyr Arg Asp Arg Gly Asp Ser 145 150 155 160 Ile Cys Ala Gly Leu Lys Tyr Ala Phe Ile Gly Ala Leu Gly Thr Thr                 165 170 175 Met Tyr Phe Leu Ala Leu Gly Thr Leu Tyr Gly Ala Phe Gly Thr Leu             180 185 190 Asn Met Ala Asn Leu Ser Ala Leu Ile His Gly Leu Ser Phe Pro Ile         195 200 205 Ala Gly Thr Thr Tyr Gly Asn Ile Ala Ile Ala Ser Gly Val Ala Leu     210 215 220 Ala Leu Ala Thr Trp Ala Phe Leu Ile Lys Ala Ala Ile Val Pro Asn 225 230 235 240 His Phe Trp Leu Pro Glu Ala His Pro Ala Ala Pro Ser Pro Ile Ser                 245 250 255 Ala Ile Leu Ser Gly Leu Val Val Asn Val Gly Val Tyr Ser Leu Ile             260 265 270 Arg Phe Leu Tyr Thr Val Tyr Gly Gly Glu Val Ser Gly Ser Leu Gly         275 280 285 Gly Val Ile His Ala Leu Ser Val Ile Leu Ile Val Leu Gly Ala Val     290 295 300 Ser Ala Leu Phe Gly Ala Leu Met Met Asn Ala Gln Arg Asp Val Lys 305 310 315 320 Lys Leu Ile Ala Tyr Ser Thr Ile Met His Met Gly Tyr Leu Ala Met                 325 330 335 Ala Val Gly Val Gly Thr Gln Leu Ala Leu Gln Ala Ala Ala Phe His             340 345 350 Ile Ile Asn His Ala Ile Ala Lys Ala Leu Leu Phe Leu Ala Ala Gly         355 360 365 Ala Phe Ile His Ala Val Gly Ser Arg Asp Ile Ser Asp Leu Ala Gly     370 375 380 Leu Gly Arg Gln Met Pro Val Ala Thr Phe Gly Leu Ala Ile Ala Thr 385 390 395 400 Leu Ser Leu Val Gly Ile Pro Pro Phe Asn Val Phe Phe Ser Lys Leu                 405 410 415 Leu Leu Phe Asn Ala Phe Met Glu Glu Ser Pro Ala Leu Ala Leu Val             420 425 430 Leu Val Leu Ser Ser Val Ile Ala Leu Val Ala Tyr Val Arg Val Phe         435 440 445 Gln Glu Ile Trp Leu Gly Lys Pro Arg Glu Asn Thr Thr Ile Arg Glu     450 455 460 His Gly Ser Met Ser Gly Val Leu Leu Ile Leu Ala Val Ile Cys Leu 465 470 475 480 Leu Leu Gly Leu Phe Ala Pro Tyr Ile Ile Glu His Tyr Ile Asn Pro                 485 490 495 Ala Val Ser Gln Ala Met Asp Tyr Arg Leu Tyr Ile Gln Thr Ala Leu             500 505 510 Glu Tyr Ala Ala Lys Leu Lys Met Gly Leu         515 520 <210> 98 <211> 195 <212> PRT <213> Thermococcus onnurineus NA1 <400> 98 Leu Ile Gly Ala Val Leu Ala Gly Gly Arg Ser Lys Arg Phe Gly Gly   1 5 10 15 Asn Lys Leu Leu Tyr Arg Ile Asp Gly Lys Pro Leu Ile Leu His Thr              20 25 30 Ile Glu Arg Leu Glu Ser Ala Asn Glu Ile Glu Glu Val Val Ile Val          35 40 45 Ala Ser Pro Glu Asn Ala Glu Lys Leu Lys Thr Phe Gly Tyr Gln Val      50 55 60 Leu Val Asp Glu Leu Leu Val Gly Pro Ile Gly Gly Ile Tyr Thr Ala  65 70 75 80 Leu Gly Leu Gly Asp Ala Phe Val Thr Ala Gly Asp Met Pro Arg Ile                  85 90 95 Val Pro Glu Phe Val Asp Tyr Ile Ile Lys Tyr Phe His Lys Ser Arg             100 105 110 Lys Ala Val Cys Val Pro Arg Trp Ile Asn Gly His Ile Glu Pro Leu         115 120 125 His Ala Ala Tyr Ser Met Gly Phe Leu Asp Ile Ile Glu Glu Gln Leu     130 135 140 Ala Leu Glu Glu Tyr Met Ile Lys Arg Ala Ile Glu Lys Ala Asn Val 145 150 155 160 Cys Tyr Leu Thr Ile Glu Lys Val Pro Phe Glu Trp Arg Glu Ser Phe                 165 170 175 Phe Asn Val Asn Arg Lys Asp Asp Leu Arg Lys Leu Gly Ile Pro Glu             180 185 190 Pro leu val         195 <210> 99 <211> 168 <212> PRT <213> Thermococcus onnurineus NA1 <400> 99 Met Ala Phe Ile Ala Ala Phe Ile Trp Cys Tyr Ile Leu Trp Leu Gly   1 5 10 15 Leu Thr Ala Gly Ser Ser Gly Leu Leu Trp Ser Ser Glu Glu Leu Ile              20 25 30 Ala Gly Leu Val Phe Ser Ala Val Ile Ala Tyr Thr Thr Lys Gly Leu          35 40 45 Ile Gly Glu Lys Ala Ser Arg Phe Leu Asn Pro Ile Lys Trp Phe Glu      50 55 60 Phe Ile Val Tyr Ser Ile Gly Pro Leu Phe Trp Gly Met Val Lys Ala  65 70 75 80 Asn Phe His Val Ala Trp Leu Val Ile Thr Gly Lys Ile Arg Pro Gly                  85 90 95 Ile Val Arg Val Pro Val Asp Leu Glu Asn Asp Ala Gln Tyr Thr Ile             100 105 110 Leu Ser Asn Ser Ile Thr Leu Thr Pro Gly Thr Leu Thr Ile Asp Ala         115 120 125 Cys Pro Glu Glu Lys Ala Leu Tyr Val His Trp Ile Asp Ile Pro Glu     130 135 140 Gly Leu Glu Lys Pro Glu Ser Ser Glu Pro Val Ala Gly Ser Phe Glu 145 150 155 160 Lys Trp Ala Arg Arg Leu Gly Arg                 165 <210> 100 <211> 87 <212> PRT <213> Thermococcus onnurineus NA1 <400> 100 Met Ile Glu Pro Val Phe Phe Tyr Ser Ala Leu Ile Ile Met Val Gly   1 5 10 15 Ala Phe Ile Ala Val Leu Arg Val Leu Leu Gly Pro Thr Val Pro Asp              20 25 30 Arg Val Val Gly Val Asp Thr Leu Asn Thr Leu Val Val Ala Gly Met          35 40 45 Val Leu Leu Gly Ala Ala Tyr Asp Arg Thr Ile Tyr Ile Asp Ile Ala      50 55 60 Ile Val Tyr Ala Leu Leu Ser Tyr Ile Gly Thr Leu Ala Ile Ala Arg  65 70 75 80 Tyr Met Gln Gly Gly Leu Lys                  85 <210> 101 <211> 123 <212> PRT <213> Thermococcus onnurineus NA1 <400> 101 Met Asn Thr Val Asp Tyr Val Ile Tyr Ala Phe Leu Ala Ile Asn Ile   1 5 10 15 Thr Phe Asn Leu Leu Gly Ser Ile Ala Leu His Arg Phe Pro Asp Val              20 25 30 Tyr Thr Arg Leu His Gly Ala Thr Lys Cys Thr Thr Phe Gly Thr Ile          35 40 45 Phe Ala Val Leu Ala Val Val Val His Ser Leu Tyr Arg Leu Asp Val      50 55 60 Thr Gly Asp Pro Lys Tyr Leu Gln Met Thr Leu His Ser Val Val Ala  65 70 75 80 Leu Ile Ala Leu Leu Leu Thr Asn Pro Thr Gly Ala His Ala Ile Ala                  85 90 95 Lys Ala Ala His Leu Ser Gly Val Lys Pro Val Lys Ala Val Ile Asp             100 105 110 Ala Tyr Glu Gly Lys Leu Gly Gly Gly Glu Glu         115 120 <210> 102 <211> 88 <212> PRT <213> Thermococcus onnurineus NA1 <400> 102 Met Asn Pro Ile Thr Ile Asp Gln Ile Ile Gln Phe Gly Ile Leu Ile   1 5 10 15 Ser Val Leu Ile Thr Ala Tyr Leu Thr Ile Arg Leu Arg Asp Leu Leu              20 25 30 Ser Ala Ala Leu Ala Ser Ala Ala Met Ser Leu Leu Leu Ser Leu Glu          35 40 45 Phe Tyr Met Leu His Ala Pro Asp Val Ala Ile Ala Glu Ala Ala Val      50 55 60 Gly Ala Gly Val Val Thr Ala Ile Val Val Tyr Gly Ile Ala Lys Thr  65 70 75 80 Glu Arg Trp Glu Arg Glu Gly Ser                  85 <210> 103 <211> 98 <212> PRT <213> Thermococcus onnurineus NA1 <400> 103 Met Lys Arg Thr Leu Ala Phe Leu Ser Leu Leu Phe Ile Leu Gly Ala   1 5 10 15 Leu Leu Tyr Ile Ala Asn Pro Asp Tyr Gly Leu Lys Phe Gly Leu Gly              20 25 30 Gly Asp Asp Trp Lys Asn Leu Arg Tyr Thr Asp Asp Tyr Tyr Ile Thr          35 40 45 His Gly Val Glu Glu Val Gly Gly Thr Asn Ile Val Thr Asp Ile Val      50 55 60 Phe Asp Tyr Arg Gly Tyr Asp Thr Leu Gly Glu Ala Thr Val Leu Phe  65 70 75 80 Thr Ala Ile Ala Gly Ala Val Val Leu Leu Arg Pro Trp Arg Arg Asp                  85 90 95 Glu glu         <210> 104 <211> 148 <212> PRT <213> Thermococcus onnurineus NA1 <400> 104 Met Thr Asp Asp Met Gly Leu Ile Val Lys Thr Met Ala Arg Thr Thr   1 5 10 15 Ile Pro Leu Ile Gly Ile Phe Gly Ala Tyr Val Val Ser His Gly His              20 25 30 Leu Thr Pro Gly Gly Gly Phe Gln Gly Gly Ala Thr Ile Ala Gly Ala          35 40 45 Gly Ile Leu Phe Leu Ile Ala Phe Gly Leu Lys Glu Met Lys Lys Ala      50 55 60 Tyr Asn Lys His Leu Tyr Ser Ala Leu Glu Gly Ile Gly Gly Leu Val  65 70 75 80 Phe Leu Gly Ala Ala Met Leu Gly Ile Gly Thr Ala Phe Phe Tyr Asn                  85 90 95 Ile Leu Trp Asn Asn Gly Pro Ile Leu Asn Gly Glu Pro Gly Thr Leu             100 105 110 Leu Ser Ala Gly Phe Leu Pro Ile Met Asn Leu Ala Val Gly Leu Lys         115 120 125 Val Phe Thr Gly Leu Val Ser Ala Met Ala Ala Ile Ala Leu Tyr Arg     130 135 140 Arg Trp Lys Ser 145 <210> 105 <211> 119 <212> PRT <213> Thermococcus onnurineus NA1 <400> 105 Met Ile Pro Phe Gln Phe Ile Thr Ala Phe Leu Met Ile Ala Leu Gly   1 5 10 15 Ile Tyr Ala Phe Leu Tyr Lys Arg Asn Leu Ile Lys Leu Ile Leu Ala              20 25 30 Leu Asp Ile Ile Asp Ser Gly Ile His Leu Leu Leu Val Ser Leu Gly          35 40 45 Tyr Arg Met Glu Leu Asn Glu Leu Pro Thr Ala Pro Ile Tyr Thr Gly      50 55 60 Tyr Glu Thr Leu Lys Ser Pro Met Val Gly Pro Leu Pro Gln Ala Leu  65 70 75 80 Val Leu Thr Ser Ile Val Ile Gly Val Cys Val Leu Ala Leu Ala Met                  85 90 95 Ala Leu Thr Ile Asn Ala Tyr Arg His Tyr Gly Thr Leu Asp Val Asn             100 105 110 Ala Leu Arg Arg Leu Arg Gly         115 <210> 106 <211> 532 <212> PRT <213> Thermococcus onnurineus NA1 <400> 106 Met Asn Gly Glu Ala Ile Leu Pro Tyr Leu Ile Ile Ile Pro Leu Phe   1 5 10 15 Gly Ala Phe Ala Met Pro Ile Val Asn Leu Ala Gly Lys Lys Ala Arg              20 25 30 Glu Ala Trp Ala Val Leu Ile Ser Ala Leu Thr Phe Ile Val Ser Ser          35 40 45 Val Ile Phe Tyr His Ile Trp Asn Thr Gly Glu Ile Ile Val Tyr Thr      50 55 60 Leu Gly Ala Glu Thr Pro Phe Gly Gln Gly Val Asp Phe Pro Ile Arg  65 70 75 80 Ile Val Trp Glu Val Asp Leu Phe Gly Ala Ile Met Val Phe Ile Val                  85 90 95 Ala Phe Val Ser Leu Met Ala Val Ile Tyr Ser Ile Gly Tyr Met Lys             100 105 110 His Asp Thr Gly Leu Asp Lys Tyr Tyr Thr Leu Ile Leu Ile Leu Glu         115 120 125 Leu Gly Met Leu Gly Ile Ala Ile Thr Gly Asp Leu Phe Asn Phe Tyr     130 135 140 Val Phe Leu Glu Ile Met Ser Ile Ala Ser Tyr Ala Leu Val Ala Phe 145 150 155 160 Arg Asn Asp Thr Trp Glu Gly Ile Glu Ala Gly Ile Lys Tyr Met Phe                 165 170 175 Val Gly Ser Ile Ala Ser Ala Phe Val Leu Leu Gly Ile Ala Leu Leu             180 185 190 Tyr Gly Gln Tyr Gly Thr Leu Thr Met Gly Tyr Leu Ala Val Lys Ile         195 200 205 Val Gln Asn Pro Thr Val Thr Ala Lys Val Ala Leu Ala Leu Phe Ile     210 215 220 Ala Gly Leu Leu Phe Lys Ser Gly Ala Ser Pro Val His Met Trp Leu 225 230 235 240 Ala Asp Ala His Pro Ala Ala Pro Ser Ser Ile Ser Ala Met Leu Ser                 245 250 255 Gly Leu Val Ile Lys Ile Gly Gly Ile Tyr Ala Ile Ala Arg Ile Leu             260 265 270 Phe Ser Ile Tyr Thr Pro Met Ala His Ser Ile Val Leu Glu Ala Tyr         275 280 285 Leu Gln Pro His Ile Asn Met Ser Ala Ile Gly Trp Ile Ile Ile Ile     290 295 300 Phe Ala Cys Leu Thr Leu Ile Ile Gly Asn Ala Met Ala Val Ile Gln 305 310 315 320 Thr Asp Met Lys Arg Leu Leu Ala Tyr Ser Ser Val Gly Gln Ile Gly                 325 330 335 Tyr Ile Leu Leu Gly Leu Gly Ile Ser Ile Ala Ala Tyr Gly Thr Gln             340 345 350 Thr Gly Glu Ile Ala Leu Ala Gly Ala Ile Tyr His Thr Val Asn His         355 360 365 Ala Leu Met Lys Ala Leu Leu Phe Leu Val Ala Gly Val Val Ile His     370 375 380 Gln Leu Gly Thr Arg Asn Leu Asn Glu Leu Ser Gly Leu Ala Lys Thr 385 390 395 400 Met Pro Lys Thr Thr Phe Ala Phe Leu Ile Gly Ala Ala Ala Ile Val                 405 410 415 Gly Met Pro Pro Leu Asn Gly Phe Ala Ser Lys Trp Leu Ile Tyr Glu             420 425 430 Ser Ser Ala Ile Phe Asn Pro Ile Leu Gly Ala Ile Ala Ile Ile Gly         435 440 445 Thr Ala Phe Cys Thr Ala Ala Tyr Val Arg Val Leu Tyr Thr Phe Phe     450 455 460 Gly Arg Pro Ser Glu Lys Val Met Ala Ala Lys Asp Pro Glu Thr Ser 465 470 475 480 Met Met Leu Pro Met Leu Leu Leu Val Leu Ala Ile Ile Ile Met Gly                 485 490 495 Leu Leu Pro Trp Gln Ile Ser Asp Lys Val Met Ile Pro Ala Ala Arg             500 505 510 Met Leu Glu Gln Phe Arg Phe Glu Tyr Ile Asn Ala Leu Met Ser Leu         515 520 525 Val Gly Gly Ala     530 <210> 107 <211> 115 <212> PRT <213> Thermococcus onnurineus NA1 <400> 107 Met Phe Gly Tyr Trp Asp Ala Leu Tyr Phe Val Phe Ile Phe Ile Ile   1 5 10 15 Gly Leu Ile Ile Ala Trp Met Leu Asn Glu Trp Ala Lys Lys Ser Gly              20 25 30 Met Gly Thr Arg Glu Ala Gly Asp Gly Thr Lys Val Phe Ile Ser Gly          35 40 45 Glu Asp Pro Asp Lys Val Ile Pro Gly Phe Glu His Tyr Glu Gly Tyr      50 55 60 Tyr Thr Gly Lys Asn Val Met Trp Gly Leu Thr Tyr Ala Leu Lys Arg  65 70 75 80 Phe Phe Ala Leu Leu Arg Asn Glu His Thr Gly Leu Leu Thr Asp Tyr                  85 90 95 Val Ser Tyr Leu Leu Ile Thr Thr Ala Phe Val Leu Gly Val Ile Leu             100 105 110 Ile Trp Gly         115 <210> 108 <211> 175 <212> PRT <213> Thermococcus onnurineus NA1 <400> 108 Met Ser Ile Lys Val Pro Ala Asp Gln Asn Arg Thr Asn Gly Thr Thr   1 5 10 15 Ser Glu Arg Glu Met Leu Glu Lys Arg Ile Ala Gln Leu Cys Arg Phe              20 25 30 Ile Gly Arg Ser Pro Trp Val Phe His Val Asn Ser Gly Ser Cys Asn          35 40 45 Gly Cys Asp Ile Glu Ile Ile Ala Ala Leu Thr Pro Arg Tyr Asp Ala      50 55 60 Glu Arg Phe Gly Val Lys Leu Val Gly Ser Pro Arg His Ala Asp Val  65 70 75 80 Leu Leu Val Thr Gly Pro Val Thr Asp Gln Ser Leu Glu Arg Val Lys                  85 90 95 Leu Val Tyr Glu Gln Thr Pro Asp Pro Lys Ile Val Ile Ala Val Gly             100 105 110 Ser Cys Pro Thr Gly Gly Ser Val Phe Tyr Glu Ser Pro Phe Thr Asn         115 120 125 Ala Pro Leu Ser Asn Ile Ile Pro Val Asp Val Tyr Val Pro Gly Cys     130 135 140 Pro Pro Arg Pro Glu Ala Ile Leu Tyr Gly Val Val Leu Ala Leu Glu 145 150 155 160 Lys Leu Ala Lys Ile Leu Lys Gly Glu Val Pro Glu Gly Glu Glu                 165 170 175 <210> 109 <211> 189 <212> PRT <213> Thermococcus onnurineus NA1 <400> 109 Met Ala Asp Asp Asn Arg Ile Met Glu Asn Val Asp Asn Val Arg Glu   1 5 10 15 Pro Thr Lys Glu Asp Thr Val Ala Glu Thr Ile Lys Ser Arg Phe Pro              20 25 30 Asn Ala His Val Glu Ile Arg Glu Asn Lys Trp Gly Arg Lys Arg Val          35 40 45 Trp Val Ile Val Pro Arg Glu Asp Tyr Lys Ala Leu Met Lys Phe Leu      50 55 60 Leu Glu Leu Asp Pro Glu Ala His Tyr Ser Ile Gly Ile Glu Gln Asp  65 70 75 80 Tyr Gly Glu Glu Ile Gly Tyr Met Ser His Ile Leu Leu His Tyr Asp                  85 90 95 Asn Ala Pro Ala Val Ser Leu Leu Val Asp Val Arg Val Pro Lys Asp             100 105 110 Asp Pro Val Ile Pro Asp Ile Ser Asp Ile Phe Pro Ile Ala Leu Gln         115 120 125 Tyr Glu Arg Glu Ala Ala Glu Met Met Gly Ile Val Phe Glu Gly Ile     130 135 140 Pro Asp Ser Arg Arg Leu Phe Leu Pro Asp Asp Phe Pro Glu Gly Ile 145 150 155 160 Tyr Pro Leu Arg Leu Asp Glu Lys Gly Ile Pro Glu Glu Ile Val Lys                 165 170 175 Asn Ala Gly His Pro Tyr Tyr Leu Lys Gly Gly Asp Lys             180 185 <210> 110 <211> 426 <212> PRT <213> Thermococcus onnurineus NA1 <400> 110 Met Thr Lys Lys Val Glu Tyr Trp Ile Lys Ile Pro Phe Gly Pro Ile   1 5 10 15 His Pro Gly Leu Glu Glu Pro Glu Lys Phe Ile Leu Thr Leu Asp Gly              20 25 30 Glu Arg Ile Val Asn Val Asp Val Lys Leu Gly Tyr Asn Leu Arg Gly          35 40 45 Leu Gln Trp Ile Ala Tyr Arg Arg Asn Tyr Val Gln Ile Met Tyr Leu      50 55 60 Ala Glu Arg Ile Cys Gly Ile Cys Ser Phe Ser His Asn His Thr Tyr  65 70 75 80 Thr Arg Ala Val Glu Glu Ala Ala Gly Ile Glu Val Pro Glu Arg Ala                  85 90 95 Glu Tyr Ile Arg Ala Ile Gly Glu Leu Glu Arg Val His Ser His             100 105 110 Leu Leu Asn Leu Gly Val Leu Gly His Asp Ile Gly Tyr Asp Thr Val         115 120 125 Leu His Leu Thr Trp Leu Ala Arg Glu Arg Val Met Asp Val Leu Glu     130 135 140 Ala Ile Ser Gly Asn Arg Val Asn Tyr Ser Met Val Thr Ile Gly Gly 145 150 155 160 Val Arg Arg Asp Ile Asp Glu Lys Gly Lys Arg Leu Ile Leu Asp Met                 165 170 175 Ile Lys Tyr Tyr Arg Ser Ile Met Pro Gln Ile Glu Glu Val Phe Leu             180 185 190 His Asp Pro Thr Ile Glu Ala Arg Leu Arg Asp Cys Ala Val Ile Ser         195 200 205 Lys Arg Val Ala Leu Glu Gln Gly Ala Val Gly Pro Thr Ala Arg Ala     210 215 220 Ser Gly Leu Lys Val Asp Ala Arg Trp Ser Glu Arg Leu Gly Val Tyr 225 230 235 240 Pro Asp Leu Gly Val Lys Pro Val Met Pro Gln Asp Val Thr Gly Glu                 245 250 255 Lys Pro His Gly Asp Val Phe Asp Arg Ala Ala Val Arg Ile Gly Glu             260 265 270 Ile Tyr Gln Ser Leu Asp Met Leu Glu His Ala Leu Asp Gln Met Pro         275 280 285 Glu Gly Lys Ile Lys Thr Phe Pro Lys Asp Asn Ile Leu Val Ala Lys     290 295 300 Leu Lys Ile Met Val Asp Gly Glu Gly Ile Gly Arg Tyr Glu Ala Pro 305 310 315 320 Arg Gly Glu Leu Val His Tyr Val Arg Gly Lys Lys Gly Ser Asp Lys                 325 330 335 Pro Leu Arg Trp Lys Pro Arg Glu Pro Thr Phe Pro Asn Leu Phe Ala             340 345 350 Val Ala Lys Gly Val Thr Gly Asp Gln Val Ala Asp Phe Val Leu Ala         355 360 365 Val Ala Ser Ile Asp Pro Cys Leu Ser Cys Thr Asp Arg Val Ala Val     370 375 380 Val Gln Asp Gly Lys Lys Arg Ile Leu Thr Glu Thr Asp Leu Leu Arg 385 390 395 400 Leu Ser Ile Lys Lys Thr Arg Glu Ile Asn Pro Glu Val Lys Gly Asp                 405 410 415 Pro Thr Pro Val Gly Phe Gly Cys Ser Arg             420 425 <210> 111 <211> 330 <212> PRT <213> Thermococcus onnurineus NA1 <400> 111 Met Asp Val Met Ala Asn Ile Iyr Tyr Pro Val Ala Gly Leu Ile Gly   1 5 10 15 Leu Tyr Ala Phe Val Ser Leu Ala Ser Leu Val Trp Glu Gly Ile Asp              20 25 30 Arg Lys Leu Val Ala Arg Met Gln Arg Arg Val Gly Pro Pro Leu Leu          35 40 45 Gln Pro Leu Tyr Asp Phe Phe Lys Leu Ala Ser Lys Glu Thr Ile Ile      50 55 60 Pro Asn Thr Ala Asn Phe Met Phe Arg Ala Ala Pro Val Leu Ala Leu  65 70 75 80 Ala Thr Ala Ile Ala Leu Leu Ala Tyr Thr Pro Met Gly Phe Ala Pro                  85 90 95 Leu Leu Ala Ser Lys Gly Asp Val Ile Val Phe Ile Tyr Leu Leu Thr             100 105 110 Leu Ile Gly Phe Phe Lys Ile Leu Gly Gly Ile Ser Ser Gly Ser Pro         115 120 125 Tyr Ala Lys Ile Gly Ala Ala Arg Glu Ala Ala Ile Met Val Ser Arg     130 135 140 Glu Pro Ala Met Met Leu Ala Leu Phe Ala Ile Ile Trp Arg Leu Gly 145 150 155 160 Lys Leu Gly Val Asn Lys Pro Phe Ser Met Glu Val Phe Tyr Gln Tyr                 165 170 175 Asn Ile Trp Glu Ile Gly Thr Pro Leu Ser Leu Ile Gly Ala Val Ile             180 185 190 Leu Leu Tyr Val Phe Val Ile Trp Leu Ala Ser Glu Ile Glu Val Gly         195 200 205 Tyr Phe Asn Ile Pro Asp Ala Glu Glu Glu Ile Ala Glu Gly Leu Leu     210 215 220 Ala Glu Tyr Ser Gly Arg Tyr Leu Ala Leu Leu Lys Leu Thr Lys Ala 225 230 235 240 Leu Lys Thr Tyr Ile Ala Ala Ser Leu Val Val Ala Ile Phe Phe Pro                 245 250 255 Trp Gly Ile Ala Asp Tyr Phe Asn Leu Thr Gly Leu Pro Ala Asn Val             260 265 270 Val Asn Leu Leu Phe His Thr Leu Lys Val Phe Ile Leu Leu Phe Ala         275 280 285 Val Gln Ser Val Phe Arg Ala Thr Thr Gly Arg Leu Lys Ile Thr Gln     290 295 300 Ala Val Asp Phe Leu Trp Lys Asn Val Phe Leu Ala Ser Leu Ile Gly 305 310 315 320 Thr Leu Leu Ile Ala Met Glu Val Ile Met                 325 330 <210> 112 <211> 152 <212> PRT <213> Thermococcus onnurineus NA1 <400> 112 Val Arg Leu Ser Pro Leu Ile Pro Thr Val Leu Arg Asn Met Phe Lys   1 5 10 15 Lys Pro Ala Thr Asn Leu Phe Pro Ala Thr Glu Pro Val Pro Val Pro              20 25 30 Asp Asn Phe Arg Gly Gln Leu Lys Tyr Asn Val Asp Lys Cys Val Gly          35 40 45 Cys Arg Met Cys Val Thr Val Cys Pro Ala Gly Val Phe Val Phe Leu      50 55 60 Pro Glu Ile Arg Lys Val Ala Leu Trp Thr Ala Arg Cys Val Tyr Cys  65 70 75 80 Ser Gln Cys Val Asp Val Cys Pro Thr Ala Ala Leu Gln Met Ser Asp                  85 90 95 Glu Phe Leu Leu Ala Ser Tyr Asn Asn Tyr Asp Asp Lys Phe Ile Pro             100 105 110 Leu Lys Pro Glu Lys Val Glu Glu Ile Lys Lys Lys Leu Glu Glu Gln         115 120 125 Lys Lys Ala Lys Ala Ala Ala Ala Ala Lys Lys Ala Met Glu Lys Lys     130 135 140 Glu Ala Gly Lys Glu Ala Lys Lys 145 150 <210> 113 <211> 324 <212> DNA <213> Thermococcus onnurineus NA1 <400> 113 atgggattcc taagcagact gttcggtggg aagaaggaaa ccgacactga ggaaattcag 60 atagtctcga ggaagccagt tggaaagttc cacgttgaga aggtcttcca catcatggga 120 cgcgaaaccc tcgtggggac cgtggagaga ggagtaatct atccaggcta caaggttaag 180 ggaaagaaag ccgcagtgat ctacaggata gagaaaggta gaaaggctgt ggattttgtc 240 gtcgacggag acaaggctgc actcattctc gagggaatta ctaaagcgga agagggagat 300 acccttgaag tctatcagtc gtga 324 <210> 114 <211> 1248 <212> DNA <213> Thermococcus onnurineus NA1 <400> 114 atgataatcg agttgaggga gttcacgaga gttgaaggca acggcaaagc cgagatagtt 60 gtcgagaacg gtgaagttaa ggacgtcagg ctcaaaatcg ttgaagggcc gcgcttcttc 120 gagctgctga ctttgggaag gcattactat gacgttccag acttggaagc gaggatatgc 180 gccatatgct acctctcaca cagcgtcgcc tcggttctgg gcatagagaa ggccttcgga 240 gttgaagttt cggaggagat ccagctccta agagagctcg gcctcatcgg tgaattactg 300 gagagccacg cactccacct gtacctgctc gttgctccag acgttttcgg ttatcctgac 360 gcgataagaa tggctacgaa gcacggggag ctcgtcaagg aggggcttgc actgaaggcc 420 ttcggcaaca gtataaggga actcattgga ggaagggaga tccacggcat aaacgttaaa 480 cccggtggat ttggcaggta tccgacggtt gaggaacttg aaaacatcga gagggagagt 540 ggggccctcc tgagactcgc aaggagagcg gtgaggctat tcgcttcgct tgagccctac 600 ggcgaaaagg cgggacactt cgtcgcgacg gacggctacc tatggggcga caagctgatt 660 tccgatgagg atggctcttt ccactacacc gagagaatag aggaacgctc actggtttac 720 agcttcgcaa agcagagccg ctataagggt gagcccttct tcgttggcgc actgccgagg 780 ctcctgctca aagcagagat gctgacaccc acagcgaaga ggctcttcga ggagcacagg 840 gaaaagctcg ccaccggtta cgtcagctac aacaacctcg ctcaggccat agagctcgtc 900 tacgcgcttg aaagggcggg agagatagca aagaaactcc tcgacaaggg cataaagggt 960 gaaaacgttc ccgttgaagt caaagaaggc gagggcatag ggtacgtgga agcgcctagg 1020 ggtgtcctaa tacatcacta ccgcattgac tctgggggca aaatcgccta ctcgaacatc 1080 ataacgccca cggctttaaa ccacgctatg atggaggcaa gcctgttcaa ggaagcgaga 1140 aaactctacg gagagacgga cgagacggta ctcgtccaga ggctggagga aacggttaga 1200 gcctttgatc cgtgcatttc ctgttcagtg cacatcgtga agctttag 1248 <210> 115 <211> 741 <212> DNA <213> Thermococcus onnurineus NA1 <400> 115 atgatggaca agctcaagtt ggccgtcttc gagcttaccg actgcggcgg ctgtgcgctg 60 aatattctct tcctctacga gaagctgttt gacctgctcg agttctacga gataacggag 120 ttccacatgg cgaccagcct aagcgagggg agccactacg acgtggccct cgtaaccgga 180 acggtctcaa gccagcgcga cctagcgctc ctcaaggagg caagaaacca ctccgactac 240 ctcatagccc tcggaacctg cgcaacgcac ggctcggttc aggctagcgt cgaggggagc 300 ataagggaga agctgaagag ggtctatgga gatgagggca acccgatgag ggcgctggac 360 tcgaagcccg tcgttgagta cgtcgccgtt gatttcgccc tcccaggctg tccctacgac 420 aaaaacgagg tatatcaggt tctgatggac attgccaaag gcattgagcc ggtaaagaaa 480 gactaccccg tctgcgtcga gtgcaagctc aacgaatacg aatgtgttct cgtgaagaag 540 ggcctcccct gcctcggtcc aataacctac ggcggctgca acgctgcttg tatacgctcc 600 gggctgggat gcataggctg tcgcgggccg ttgcccggcg aggtgaatcc tgcaagtgag 660 tacgagatac tcaaggatct gggctacgat gatgactaca tcctcaggaa gttcaagacc 720 ttcgcgaggt gggagccatg a 741 <210> 116 <211> 876 <212> DNA <213> Thermococcus onnurineus NA1 <400> 116 atgagcgaga atccacatca aacttacgat gcgcgcattc tggaagtgaa ggacctaaca 60 cccagggaga agctcttcac gctccgcttc ttagacccgg aaattggcga acacttcaca 120 ttcaagcccg gccagttcgt catcgtcgat atacggggct tcggtgagtt ccccataagc 180 ctctgctcct caccaacgag aaaaggatac attcagctct gcatcagaaa agccggaagg 240 atgaccaagt tcatccatca gatgaaagag ggagaagtgg tgggcatccg cgggccctac 300 ggcaacggct tcccgatgga gaaaatggag ggctcgaatc tactcctggt cgccggtgga 360 ctcggtatgg cacccctccg ctcggttctc tggtacgcga tagacaccgg aaagtacgag 420 cacgtctggc tcctctacgg caccaaagcc tacgaggaca tactcttccg cgacgagata 480 atccacctgc tgaagcacgg cgacgcggtt ggctgcagcg taaagctcgc ctatgaggtc 540 gaaagcccct cgtgcatcta cctcgagcgg ggcttcttcg acagggtgtg caagggtgtc 600 gttaccgacc tcttccgcgg ggaggagttc gacgtcgaca aggcttacgc cctcatctgt 660 gggccgccgg ttatgtaccg cttcgtcatc aaggagctcc tagacaggaa actctcgccg 720 ggcaggatat acatgaccct cgagaggcgc atgcgctgcg gaataggcaa gtgcggccac 780 tgtatagtgg gaacgagcac ctccataaag tacgtctgca aggacggccc cgtcttcaca 840 tactgggatg ctctctccac gagggggttg atatga 876 <210> 117 <211> 1005 <212> DNA <213> Thermococcus onnurineus NA1 <400> 117 ttgagatatg taaaactatc atctgagaac tttagctcat tttttgaatc tctaaggaat 60 tggggcaaag tctacgctcc catcaaaaga ggaagcattt acacattcca agaagttcac 120 gagctaggag agatagaact caactataca aggacaatgc tacctccaaa aaagttcttc 180 gtgaggccaa gggacgaaat tcttcgcctg aagaacggtc gctgggaaaa tggaaccgac 240 gcagagccga tagttctctt cggcctccac tcctgcgata tgcacgggct caagattctc 300 gataaggtct atctcgacga gcccgccgac ccgtactaca aggcgaggcg cgagaaaacc 360 ttcatagttg ggataagctg catgcccgac gagtattgct tctgcaagag tctcggcacg 420 cactttgcca tggacggctt tgacctattc ctgcacgagc ttcccgacgg atggctcgtc 480 aggataggaa gtgtgagggg acacgaggta gtctgggaga acggtgagct cttcgaggag 540 gtgaccgacg aggacttgaa gcacttcaag gagttcgagg agaggcgcgc aaatgcgttc 600 cagaaggaga tcccgcagga aggactcgca gacatgctcg atttggccta caacagcccg 660 gtctggaagg agtacgccga gatatgcctg gcctgcggca actgcaacat ggtctgtcct 720 acctgccgct gctacgaggt ctgcgataac tggatcagcg cctacgacgc cgtcagagag 780 agacgctacg actcctgctt tatggagaac cacggactgg ttgccggagg ccacaacttc 840 aggccaacta gactcgacag attcaggcac agatactact gcaagagcta cttcgatccc 900 tcctcgggtt ataactgcgt aggttgtgga aggtgtgacg agttctgccc ggcgaagata 960 gagcacgtca aggttcttga ggaggtcagg gggtcgctga gatga 1005 <210> 118 <211> 420 <212> DNA <213> Thermococcus onnurineus NA1 <400> 118 atgcacgaat gggctctggc tgatggaata gttagaaccg ccctcgacta cgctcaaaga 60 gaaggtgcaa aaaagcttct ggcaattcaa gtagtcctcg gagagctcca ggacgttaac 120 gccgagatag tcgagtttgc catgaaggag cttctcaagg gcaccatcgg ggagggtgcg 180 gagatagagt tcatcgagga ggaggcagtt ttcaagtgtc gcgactgcgg tcacgagtgg 240 aagctcaagg aagtgaaggg aagcttcgat gagcgcatca aggaggacat ccacttcatc 300 cccgaggtag ttcacgcctt cttggcatgt ccaaagtgtg gcagcaggga cttcgaagtc 360 gtacagggta ggggagtcta cataagcggg ataaaaatcg agaaggaggg ggaggcatga 420                                                                          420 <210> 119 <211> 747 <212> DNA <213> Thermococcus onnurineus NA1 <400> 119 atgatagcca tagaccctcg cgtcaagggc atagaggctc gccttgaaaa tgtgaagcgc 60 atcattcccg tcgtcagcgg caagggcggc gttggaaagt cgctggtttc aacaacgctc 120 gcccttgttc tggcggagaa gggctataag gtcggcctcc ttgacctcga cttccacggc 180 gcgagcgacc acgtaatcct cggcttcgag ccggaggact tccctgagga ggacagaggc 240 gtcgtcccgc cgacggttca cggaataaag ttcatgagca tcgtctatta ctcggaggat 300 aaaccaacac ccatgagggg catggagata agcgatgcgc tcattgagct cctcactata 360 acccgctggg acgagctgga ctacctcgtc atcgacatgc ctcccggtct cggtgatcag 420 ttcctagatg tccttcgctt ccttaagagg ggtgagttcc tggttgtggc aacaccttca 480 aagctctccc tcaacatcgt caggaagctt cttaccctcc tgaaggagag ggactacaag 540 attctcggta tcgtcgagaa catgaaactt cattcggagc aacgaaacaa cgagaagggc 600 atagaggctc ttgcagagga gttcgacgtg ccctatctgg ctggtatccc gctctacgac 660 gacctcgagg agaagatagg aaagcccgag gagctcctca ggacggagtt tgcagagagg 720 ataagggaag ttgcggggaa gctctga 747 <210> 120 <211> 477 <212> DNA <213> Thermococcus onnurineus NA1 <400> 120 atggagcttg aagaactctt tcagaacgct aaacgtatcg ttatctgcgg catagggaac 60 gaacttaggg gggacgacgc ctttggtgtc cttgtggcgg agatgctggc ggagctcgtc 120 aaaagcccga acgtcttagt catcaactgc ggtgaagtgc ctgagagcta caccggaaag 180 atagtggatt tcaagcctga tctgattatc ttcgtcgatg ccgttgactt cggcggggag 240 cacggagagc tcgtagtcgc cgatccagag ggaacactcg gtgaggcggt ttcgacacac 300 agtctgcccc taaaattctt agtccagtac ctcaaaacga aggtaaacgc aaagttcgtc 360 ctcataggct gccagccacg ctcgctcggc cttttcgagg agccaagtgg ggtgataaga 420 gagagggctg agatgctggc acaggcgatt gcagattcgc ttaatggggg cgaatga 477 <210> 121 <211> 582 <212> DNA <213> Thermococcus onnurineus NA1 <400> 121 atgataggag cagttttggc cggggggagg ggcagacgct tcggcggcaa taagctgctc 60 ttcaggatag acgggaagcc gcttgttctt tacgccgtcg aagcccttga agccaccaaa 120 gaaatagatg ggatcgtcct tgtggcctct cccgagaatg cagaacgtct aaaaaagttc 180 ggctacgagg tcatcgttga cgagctgatg gtcggcccca taggcggcat ctacactgca 240 ttgagcctcg atgatgcctt cgtggtggct ggcgatatgc ccctgatagt cccagaattc 300 gttgattaca taattcgtga attcaggaaa agtgggaaag cagtctgcgt tccgcgctgg 360 agtaatggat acatagaacc cctccacgcg gcttactctg agagattcag aaaaatcctt 420 gaggaacaga ttaagagcgg agactacatg ataaggaaag ccatagagag agtcgacact 480 tgctacctgc cgatagagga gctcccagag aggtggcgca agagcttctt caacgtgaac 540 aggaaagagg atttacggaa gcttacctat acctccttct aa 582 <210> 122 <211> 441 <212> DNA <213> Thermococcus onnurineus NA1 <400> 122 atgagcattg aggcggcaaa gcagagtctt cttcagcgga taagggaatt ctacggcgac 60 aatttgctct ccgtggtctt ctatggggca cacctgaagg gagggcttga tgagattgat 120 gtgctggtta taatcgatga accctacgac ccggtcaaga tcaaccgggt ggcggacttt 180 atagaaaaca tcaaggaacc cgttgaggag gaatacggcc actttatagc ctttgagctc 240 tacacgaggg aagaggcgga gaacttccac tcgtccttcc tcgacgttgc caaggcccac 300 gagattgcct acgacaggga caactacttc cagaacctgc taagagagat gctaaatccg 360 aagaagacta tagagtacgt gaagtacata agcaccatcg agtacatccc agttgatgag 420 gagaaaaggg aggagcaatg a 441 <210> 123 <211> 1566 <212> DNA <213> Thermococcus onnurineus NA1 <400> 123 atggacgtgg ttggactaac tcctgtgatt ccggtcatct ttgcctttgc actccccctg 60 acttcgatca tagttaaggg caacaggaag ataatccaag cctatgcact gctcggcacg 120 ggtttaacct taatcagtgc gctcaagctt ttcgaactag tgtactcatc tgaaaagccg 180 ccgatatata cttttggcgg ctggactgct ccaattggca taatttatga ggttgacaga 240 actggggcac ttataactct cctcaccgcc gcgctgatgt tcctcattgc gatttacagt 300 tatcgctacc tagagcacga ggagggtctg gagtggtact atacactcta tttaggcttg 360 gaagctggac taataggtgt tttactaact ggtgatgcct tcaacctctt cgtcatgata 420 gaagtcacca gcatcgcagc atacgccctc gtagctttct acaggggaag gaaggacgca 480 gttcatgctg cattgaagta tgcattcata ggtgcaattg gtactacaat gtacttctta 540 gcgcttggaa ttctctacgg ggcttttggt acggttaatc ttgctgattt gagtgcaaag 600 gttcatggtt tgagttttcc agtaacaggt tcaccagttg gtaatattgt aattgcttct 660 ggtgttgctt tggctttagc tacgtgggct ttcctcataa aggcagcaat tgttccgaac 720 cacttctggc ttccagaagc tcacccagcg gcaccaagtc caatctcggc agtcttgagt 780 ggtctcgtag taaatgttgg agtttacgct ctgattagat tcctctacac tgtttacggt 840 ggtgaactca caccgggtct agcaaaaatc gttggaacct tggggacagt agtcatagct 900 ctcggtgcgg tttcggcact tttcggtgca ctcatgatga acgcccagcg cgacgtcaag 960 aagctcattg cttactccac gataatgcac atgggttacc tgttcatggc cgttggcctc 1020 ggcacccagc ttggacttca ggcggcatta ttccacatta tcaaccacgc aattgctaaa 1080 gcattactct tccttgctgc gggcatgttc atccacgcca tcggttcaag ggacatcaat 1140 gatatggctg gtttgggcag acagatgcca atggcaactc tcggactcgc aatagcaacc 1200 ctcagcctcg ttggaatccc accgttcaac gtgttcttca gcaagctgct cctctttgat 1260 gcgctgatgg agaagagctt agccttggcg atggttatcg ttgtcagctc agtaactgca 1320 ctcgttgcct acatgcgcgt cttctacaag atatggctcg gcaagccatc cagagaagtg 1380 gcagtgggag aatctaagag catggcattc gtggtgctcc tgctggcggc tgcggttgtt 1440 gtgataggtc ttgccgcgcc ggtaatactc cagcactaca tcgaacctgc cgcgacccag 1500 acgatggact acaagcttta catccagacc gccctggagt atgcgtcaag attaaaactc 1560 ctctaa 1566 <210> 124 <211> 486 <212> DNA <213> Thermococcus onnurineus NA1 <400> 124 atgagggggg tcattccaac ggccctgctc gcgttcatca cgtacataat cttctctggt 60 tcaagcagcc cctacgacat gttcactgga gcagttgtgg caataggagt ggggttgctg 120 atggggagat acgttgtcca gaatgatgcc aaggccatga atccagtgag gtggctgtgg 180 ggagtcattt acttcctctg gtatatgctc gtggcagaga cgaagtccca catagacgtc 240 ataatcagga ttatcaccgg caactacaac cccggaatag tgaaggtacc tattggtgtc 300 gagacgagct acgctaagac cctcgtggcg aactcgataa ccaacacccc tggaacggtc 360 gtggtggaca tggacgacaa gtacttttac gtaaactgga ttgacgtgac gactctggac 420 cctgagacgg ctaagggaga aatctcggcg gactttgaga gatttgcaaa gagaatactg 480 gagtga 486 <210> 125 <211> 393 <212> DNA <213> Thermococcus onnurineus NA1 <400> 125 atgagcactt cagagttcct gtgggcttac ctctggaccg tcttactgct cacgttggcg 60 gtatcgctgt atggcataat cgctaggccg aacatgctga agaaaatcat atctctcacg 120 atactaggcg atgccgtcaa cgtcatggtc gttctcatag gctaccacct cacctatcca 180 atcgccccac cgatactacc atcgctctca aaggaagcgt tggaagggtt cgtgagctct 240 gccgttgatc cacttccgca ggcactagtg attaccgccg tcgtcatagg aatggcggtg 300 aacatgctcc tcgcggtgct agcgatacag ctctaccgcc tctacggcac gcttgacgtg 360 agggagatag cgaagagggg tggtgaggaa tga 393 <210> 126 <211> 774 <212> DNA <213> Thermococcus onnurineus NA1 <400> 126 atgaggggca aaagcctcat cacggcggtt ataatcctga ccttcgccgc gctcatgact 60 tacgctgtta tctcactcca ggtcttcggt gagggtactg gagttaggcc ccttggcgag 120 ttttaccttg aaaacagcta tttcggagat tactcggcca agagcccgga ggttatcacc 180 tcaatcctct gggactaccg tggcattgac acgctcttcg agactgcagt gttcttcctc 240 gcaataatag gcagcctgac cgtcttcagg ctcaccaagg agcaggagaa agaagtcaag 300 aaggctgaaa gcactccaac cgagctgacg ccaatagtga aggacgttac taaagttatc 360 gtcgtcatga ttcttgccgt ttcagcttca atagccctgc acggtcactt aacgcctgga 420 ggaggcttcc agggcggttc ggcattggct gtggcaccgc tcctcataat agcggcatac 480 tcaaaataca ctctggaagg ccacggcctg gacaaaacca gagctttaat ccttcgttcc 540 atcggactgc ttggcatagc cctggttgct ctggttcccc tcctcagcgg tggcttcatc 600 atgcagaacc agccaatatt cccggctgag attgagagtc agcttatcgg tggttcccta 660 atctactaca acttcttcga gttcctggct gttggcgttg gattcacggc ggtgttcctc 720 cttctgagca tcccagagga gaagttcaag aaaatcctgg gggtgaagaa atga 774 <210> 127 <211> 255 <212> DNA <213> Thermococcus onnurineus NA1 <400> 127 gtgattgaag ttcatctgct catcctcagc cttgccctca tcctgggatt catagccagc 60 tatctagcgg tgatggagag ggatctgctt aaagcagtgg gcttttcatc cgtccaggca 120 atagcctacg caatagcctt ctacatccta atggcgccgg acatagttct cacctacata 180 gcgatagccg tgggaatata ctccgccctg ctgatattcg ccatcagcaa gaccgagagg 240 tatgaggtgg tgtga 255 <210> 128 <211> 357 <212> DNA <213> Thermococcus onnurineus NA1 <400> 128 atgagcattt tattctacat aggagcgctt ctgatagtca taggcgccct ctgcgatttc 60 ttcggagcat taggattact ccgcttccct aacttctacg ttaggctgca cgccgcgacc 120 gtgggcatca tcggcggtgc agcggttccg ctcttcggcg ttgccctgct tgcccttggt 180 gcggacttcc taccccacag gtatgccata gcgggagcga gcttcatcac cggcataata 240 gtcctccttg cctccccagc tggaagccac gctttggcct atgccgctca cagggccaag 300 ctcgtggagt gggagcccaa agtggatcac ctcggggagg tgaggaagag tgattga 357 <210> 129 <211> 285 <212> DNA <213> Thermococcus onnurineus NA1 <400> 129 atgagccttg agtctcagtt cttcctactg atgaagtttg tgatacctgt ttatctgctg 60 gctttcatca tctatgccgt cagagccttc aagggtccaa cgatagcgga tataatcctc 120 gccgttgact gtctctcctt cgatgtggca gcgttcatgg ccatccttgc ggtttacttc 180 aagagcgttt acttagtgag cggggcgatg attttggcac tgtggggcta tctgctggat 240 atctacatcg ccaagcacct agtgagcaag gaggtgggag catga 285 <210> 130 <211> 306 <212> DNA <213> Thermococcus onnurineus NA1 <400> 130 atgcctacga aaagaggcac ctggcacttg ttaaagctgg tgtacgttaa tccaccttac 60 gtctcgaagg cgatggcaca tttcgccggc gacatggctg tgttagagga aataggcacg 120 ctgaatgaga aaagcgtcga gataatcaag gagagcctcg gggcaaaggg aaacgagata 180 gacctgtcta taggcgacct tatactgata ccagtggaaa ggttgcccga ggacgatagg 240 aagctcctct taaaagtcgc ccaggagctt ggggcggacg ccaagctcaa gatagtgagg 300 gggtga 306 <210> 131 <211> 801 <212> DNA <213> Thermococcus onnurineus NA1 <400> 131 atgagcgggt tgaagtccgt ttgggtcttc cacgttgaca gtgggagctg caacggctgc 60 gacatagaga tactcgacgt gctcacgccc tattacgacg ccgagaggct tgggataaag 120 ctcgtgccga gtccaagaca tgccgatgcc ctcctcgttt caggcccact cacgaggcag 180 acttactacg ctgtcaaagc agcctacgag gcgatgccgc cgaagccgag gatagttgtg 240 gccataggca cctgcgcgtc cagtggtggt atattctaca acggttaccc aatctacaac 300 ccgaaccctg agaggggaag cgacaggctc aggacgggtg gaatagaggt ccttttggcg 360 gagtacggga aaaagcccga catgtacatt ccaggatgtc caccgagtcc ggaggagata 420 ctatatgggc tggcccagct cctcggcctg aaggagaaga agatgaaggg cgagtactac 480 tatgcagacg agattgagtt cgttcttcca gagagaccca tcgaggagag gatttacctg 540 acgctcagag aatccctgag gcgcgtcgtg gggtacttcg acagggagaa ggttctcgag 600 gacttcatgg ccctcgtgga aaaggctcag gagagcgaga acccgaggga gaggctccac 660 gagctagtca tcggatactt cctgagggag aaggattccc gtgtgaagtt cgcgataagg 720 ttcctcgaaa acgagtactg gaggttgaag gatgcctacg aaaagaggca cctggcactt 780 gttaaagctg gtgtacgtta a 801 <210> 132 <211> 618 <212> DNA <213> Thermococcus onnurineus NA1 <400> 132 atggcccagg cgatttcctt caccgacagg ctcaagttct ggaagcgacc agaggaggac 60 gttaagaagg ctcccgtcac gacttcttat ccttttgttg atatcgaaaa gccgccggaa 120 tataggggca tacctcgcat agatcctcac ctctgcattg gttgtggagc ctgtgttagg 180 gcctgtccac cggacgcgct cacgatagag tgggacttcg agaacgggag gaagaggata 240 gtcttcaacg ccgcgcgctg cataaggtgc caccgctgcg tcgaggtttg tccaaccggt 300 gcgatgcagg gcacaacgag gttcgagata gcgacgccga acaaggagga cctcatcgag 360 gtcgttgacc acaagctcta cagatgcccg cgctgtgggc ggtacgagga gttcaccgag 420 aggcagatag ggaagatgtt ccagattctg ccggaggaag tcattgacca gcacggcata 480 gctgagaggg cttttctctg cagggagtgc aggatggagg agagcgccaa gaccttggcg 540 gttcaagggc cctatgcgga tagccttctc ctttccctct atccgagagg ctcaaaggtg 600 atgggtgaga ggagatga 618 <210> 133 <211> 1752 <212> DNA <213> Thermococcus onnurineus NA1 <133> 133 atggagtgca gcgtgtgtgc gggtggatgc agatcggctg aagttgagga cgtccttgag 60 gatggtcatc taaaggaatt cgtggagaag tttaggggag cgatcttcga gtgcaagaag 120 ctgacgagga accagtacct gttcatcgtt gatagggagg cacttccgga gatggtcctc 180 cactggcaca accattccga gctaaaagaa acccacttct cgatgggaac aggaaccgat 240 gagaggaaca tcgccggaaa gttcacctac gctccggtaa taaacgttgc cgttgagcct 300 ggaaacgggg agaggaacta ctgggttatt ctgaaggcct acctcgacga ggacaacccg 360 gagttcccct ccatagccgc gaagcttcca gcagccctct gggcggagag ggaagtctat 420 gatctgcttg gcttcaaccc caaaggccat cccgacctga ggaggctcgt cctgccggag 480 gactggccgg agggtgttta cccgctcagg aaggaccatg actacaaggc ctcgccgatg 540 gatacgccaa agtgctacta caagcccggg ccgcccgaca caatgacggt tccgattggt 600 ccgtaccacc tggcgctcga cgagccggcc cacttcagga tattcgtcaa gggggaaacg 660 gtggttgacg ttgactaccg cggcttctac tcccacaggg gaatcgagaa gataggagag 720 ggaagactga cctacaatca ggtgctcttc atagccgaga gaatatgtgg aatctgtggc 780 ttccagcact cgacgagcta cgcccaggcg gttgaaaaca tagccggcgt tgaaatcccc 840 gagagggcca tgtacataag gacgataatg ctggagatag aaaggattca ctcccacatg 900 ctctgggccg gtgttgcggc tcacctgacg ggctttgaca cgggattcat gcacgcttgg 960 cgcgtcagag agcctgttat gtggctcgca gagaggctca caggaaacag gaagacctac 1020 ggaatcaaca tcgtcggagg agttaggagg gacttcctcg actaccgcaa ggagatgata 1080 atggagaaga tcaaggagct caggaggcag gtcgaagagt tcatcgaaat agcgaccggt 1140 acggcaacct tcgtcaagag ggccgagggg gttggaattc tgccgtacaa ggtggccaag 1200 gcttactcag tccttggtcc gaacggaagg gccagtggga ggaacattga cattagaagg 1260 gatcagccgt tcgcagcata caaggatttg gacttcaagg ttccagtcta caaggagggc 1320 gacgtcttgg caaggttcct catcaggatg gacgaggtgc tcgagagcat ctggataata 1380 gagcaggcca ttgaccagat gccgggagga gacgtcttcg tgccgatagg ggagcttccg 1440 gagtatgaag aggccctagg ctacagtgaa gctccaaggg gcgaagtcat ccactacgtc 1500 atgactgaca agaagaacaa ggtctaccgc tggaaggtta gagccccgac ctacaacaac 1560 cttccagctg ttccggagat gctcaagggc tacagcgttg ccgatgcccc gctcatcata 1620 gcgagcatag atccgtgcta ctcctgtacg gagagggttc agatagtgga cgttgagacc 1680 ggaaaggccc agaccctgaa cgagcagcag ttcaacatgc tctcaataca gaagggcaag 1740 ggggtggcct ga 1752 <210> 134 <211> 903 <212> DNA <213> Thermococcus onnurineus NA1 <400> 134 atggactacg taagcattat cgctgctccg atcgtcctct tcctccttcc accgttcctt 60 gacggaatag ggagaaggat aaaggcgagg attcagtaca ggagaggacc gcctataatg 120 cagacgttct acgacctcga aaagcttctc aagctgccgt cagtgcttcc aactgagggc 180 ccaatcttca ggctggcccc gtacatagcc ctggcatctg ccattgccgg cggcctaatg 240 cttcccttcg gaagcgagcc ggtgttggct tttggaaaga gcctcatagt gttcttctac 300 gtcatggcga tggtcagcgt agtgatgata cttgctgctt tctccgtcca gaacgcgttc 360 tctcacatag gtggacacag agaggtcatg ctgatactct cgattgagcc agtgctggcc 420 gtcgtcttcg gtgtcctggc attcaagctt ggaacgctca acgtcgctga gatgcctttc 480 agtgctaacc tctcgctttc cgttgcccta gcttacatct tgctggctta cgcggtctac 540 gttgagggcg gattcgttcc atttgacata gctgaggcag aaaccgaagt aatcgggggc 600 ccgctcaccg agtacagcgg aaggctcctc ggagtcttca agtacgccct gctcgtcaag 660 agggttgtcc tgctctggct gctggcgtct atgattgtga ttcccgccat gaggtctctc 720 ggtataacaa gctcaatggc actgctcgtc gcccagctgg tcgttacgtt tctgctttat 780 tcgcttgccg tggccgttga ggctgcaaac gcccgcctga ggatcgacca ggcggtttcc 840 cttaacaaga aggtcttcct gatgtccctt gctgtcctga taatagcgct ggtggggtgg 900 tga 903 <210> 135 <211> 1980 <212> DNA <213> Thermococcus onnurineus NA1 <400> 135 atgatggaaa ttccaatcgc gctctactca ctctcagcga tttccggcct gattggagac 60 tttaagcgga gcattaagat ttcaagcgtc ctctcagcca tagcatccct atcccttctg 120 ggcatagctg cccacgccct gtccaggggg cttcccgttc aggagagctt tttgggcatt 180 cccctaatca tagacagcct ctccctcccg ttcctgttca tcatagccct gctcagcctt 240 gtggtttcag tgtattccat ttcatatatg gaagtccaca gagataccgg aagaccactg 300 gcgtacacca tcctctacgg cacgttcgtg ctgtcgattg tattcgtggc tctgacgtca 360 aacctgctct ggttcgtctt cttctgggag ctgatgaccc taacttcctt catcttcgtg 420 agctggaggg agcaggacgc tggaattaaa tacctcctca cgatgcagct cgccaacacg 480 gtgcccctct tcgtggccct cggcataatc tactccgcca ctggaagctt cagcgttgat 540 tacgccacgc ttagggaggt tgcatcttcc ctttctccag tccagctcaa gctgctctac 600 gcgatgttcc tcgtgacgtt ccttgcaaaa tctggaagcg tgcccttcca gttctgggtg 660 cccgatgcat acgaagccgc tcccagcaat atagcctcgc tgatggccgg cgtcatggag 720 aagatggcgg tttacggtct gataaggctc ctctgcaacg ccctgccatg cagtgaaggc 780 attggttacg ttctcgttat cgtcggcata cttaccatga ccttcggaac cctctacgcc 840 ctcagagaga ctcacgcaaa gaggctcctc gcttactcaa gcgttggaca aatgggctac 900 atctggttcg cggtgggcat gggcatgatc ttcctgacga tgggcatgga gagcctggct 960 tacctggcct tcctcgccgg agtcttccac tccttcaatc acacactctt caaggggctg 1020 ctctttctca tctcgggcaa cttcgagtac tccgccggaa ccgctgacct caacgagctt 1080 ggtggtttga ggagggcaat gccgtactcg tcgctcttca ccgtcatagg tgcgctctcc 1140 ctcgctggag tgcccctctt cagcggtttc ctctccaagt ggatgattta ccaggccggc 1200 tactactctg gaatcggcct cttcgtcttt ggctccgtaa tggcggtgtt tatgagcgcc 1260 gtaaccttgg catattcgct caagctctac acctctgcct ttgggggcga accgaacgag 1320 agaactgaga acgccaggga agtcccgtcg ggtatgctcc tcggtgaggg aattattgcc 1380 ttaacttcac ttgccgttgg aatacttccg gctattgctt acccgatatt aacgatttca 1440 ttgaatggcg gcgacgtcac cgttacaatg ggctcgatat ccactgactt tgagtacttc 1500 tcgccaatag ccctgctcct tgcggtttca ttcattgcgg ttgcttcata cttcgtcttc 1560 aggccaaaga cgaccaatgt caaaccctgg aacactggag cgcttttcct gccggaggag 1620 aggtatggag cgaaggccag ggactattac aggcagtact ttaccgagat ggagggcctc 1680 tacaagcttg gaagcgccgc tggcaaggtc ggaagggtcc ttctctctgc tctgatgtcc 1740 gtctacctcg ttctcgccag gggcctcgtc tacaccggca gggagaagaa gcgctccttc 1800 acccttgacg agcttcgcca ccgcaccgtc aggtacctgg acgaggcatt cttcgcgccg 1860 atgatggatc tactcaaaaa catcgccgtg ctggcagcgg gcatctcggt gtccatggac 1920 gagctcttcc tggcttcaat gctgaccacg gtgataatac tcgcactcct tgtgttgtga 1980                                                                         1980 <210> 136 <211> 1452 <212> DNA <213> Thermococcus onnurineus NA1 <400> 136 atgaacgcct ctcccttcat tatatctttt ttgatccccc tgctcctcgg tccactcctg 60 ttcaaattag acggtagaaa ggccgatgta ttcatgctca tcgccgttgt gtcttccttc 120 ctggctaatc ttgtgggagt cctcgaatac ctgaaagtcg gtggcgctca tcatatcgtt 180 tacctcgaaa cttcttccct cggtgaggtc tacggcgtta taatcgaccc aatgagcgtt 240 ttggtcggtt ttgtcgtgag cttggctggc gtgctgttcc ttctctacgc ggtggactac 300 atgagcgaga gaaacaagca gcaccccgtc tactctgata agggcaggtt ctatgcttgg 360 atggtcatct tcgttggagc tacgctggca ttcatatact cctccacgac gcttcagctg 420 ctcatattct tcgagataat gggactcgcc tgctggggtg tcgttgggta ttataagggc 480 ccaaaagccg agagggctgc atacaaggcc ctgcttgtgc cgaacttcgg tgccatggtg 540 ggcctctaca ccaccgttgg cattggcatc ctcaagctcc atgatttgag catctatgcg 600 ctccagaacc tgaatgatga gctcaagctt ctcgtgttcc ttggcgtaat ggttgcggcc 660 tttaccaaga gcgcccagtt cccgctctat tcatggcttc cggatgcaat ggcggcgccg 720 acacctgctt ccgcttttct ccacggtgct gcaatggttg aaatgggcgt ttacctgctc 780 gccagggtca cccagttcat gcaaccgatt ccggagacag ctttctacgt tatgctcgtc 840 ttcgtgtcgc taactttact catagcaatt ctctactacc cgctccagaa ggacgccaag 900 agactccttg cttattcaac catagcagag gcaggagtga tgtacgttgg cgtgctctat 960 gccgtgcttg gctctgtcta tggtctccag gcggccatgt tccagctggc taaccacgct 1020 ttcgtcaagg gtcttgcctt cctcaccgcg ggaaccttca gttacgcttt tggaacgctc 1080 gacatggaga agattagggg cctcggaaag ctcgttccgg tcgttggtgc aagctggttc 1140 ttagcccttc tcggcctggc tggagttcct ccgctcggcc tgttcttcag caaggcgtat 1200 ctcttcatga acgcgtcttc aataaccagc tgggttggct ggattccgct cttcctagtg 1260 ttggccgatg ccacggtttt ccttgcggta tctctcggat ggattaagag gatggtattc 1320 agcgagcccc tccaggagag tgcagaagtt tccccgctga tgcgctttgt cctcgtagtc 1380 ctaatagtcc tgtccatcgt tgcgccgttc ctaagcgtga agctcgtgac tcagataggg 1440 ttcatggggt ga 1452 <210> 137 <211> 498 <212> DNA <213> Thermococcus onnurineus NA1 <400> 137 atgagcaaaa agatatttat cgattttaag cgctgcattg cctgtaaggc ctgtgaagtc 60 gcctgtgaaa tggagcacgg ggaagcgagg attagggttt ttgagttccc cgatctgacc 120 agcgtcgcct tcaactgccg ccactgtgaa aaggctccat gtatggaagt gtgtccagtt 180 aacgcgctct ccaaggacga tgatggcgca gtcgttctcg atcccctcaa gtgtatcggc 240 tgtctcatgt gcggtctggc ctgtccattc ggcattccaa agatagacga gtacaacaag 300 ataatggaca agtgcgacct ctgtgcccac aggagagccg aaggaaagct tcctgcctgt 360 gtctcagcgt gcccaactga ggccctcaag tacggcgaca taaacgatgt cctctgggcc 420 agagaaggaa agatagtcgc cgagcttaag gacatcggcg acaggaccaa cgtcctcgag 480 gcctacctca tcagatga 498 <210> 138 <211> 2070 <212> DNA <213> Thermococcus onnurineus NA1 <400> 138 atgggtatgg cagaaaagcg catatcagtg gtgtgtccgt ggtgttccgt tggttgtaga 60 ttttacatag taaacgtcaa tggctaccca aagaagatcg agttcgacta cgaccacgac 120 atcaggaacc acggcaagct ctgtccaaag ggtgtcgcag ccttccagca tctcaggcat 180 ccagacaggc ttaaaaagcc ccttaagagg gttggcgaga ggggtgaagg caagttcaag 240 gaaataagct gggaagaggc tattaaggaa atcgcacaga agctcagtga aatcaaggag 300 aagtatggtt cggaggctct tgcttttctc ggaagtgaaa ggtgctccat agaggagaac 360 tacgttcttc agaagctggc aagggctttg ggaaccaaca acattgaata tgtgtgtagg 420 atgtgtcagt caacggctgt tgcaggtaag gggatggttc ttggacaccc cggtctgacg 480 aaccccttcg aggacattct taaggccaaa gttatcgtcc tttggggata caatccagcc 540 gcaactaatc cggtcttctt cggccagtac attgagaagg caattctcga caacaacgcc 600 accctcattg tcgttgaccc aagaaaaacg aagactgcca agtacgcaga catacacctg 660 cagccatatc ccggaaccga ccttgccatt gcgttggcta tgctcaacgt cataatcacc 720 gaggagctct acgataagga cttcgtggcg gagcgcgcgg agggccttga ggagctcgcc 780 aagaccgtcg aaaagtacac tccagaatgg gctgagaagg tcagcggcgt tcctgccgag 840 ctcataagga aggccgcaat cacctttgca acggctggaa ctgccgccct gctgacgaac 900 gagggagtga accagcacgc caacggaacg aggactgtta tggctatcac tgagatgatg 960 gttctctgcg gctacttcgg aaaggagggc gtcatgtctg gagctatacc cggtgcccac 1020 aacggtatgg gcgctggtct aatgggtatt ggaccacacg aactgccagg aagattcccg 1080 ctccacgccg aggagcacaa gaggagaatt gaggaggcat ggggcttcaa gatcccagag 1140 aagcctggaa tcacttacgt tgaaatgatt gatgcaatcc ttgagggcaa gctcaaggcc 1200 ctctacgtca tgggaaccaa ccctgccaag gcccttccga acctcaagaa ggctgaggag 1260 gcctttaaga acatcgagtt cctcgtcgtc caggatatct tccttactga gaccgcgaaa 1320 tacgccgaca tagtccttcc agcggctgca tggtttgaga aggacggaac cgccataagc 1380 ttcgagagaa gggttcagag gagctttaag gctgctgacg caccgggaga ggccaagcct 1440 gactgggaaa tccttgttat gctcgctaag gagctcggct ttggagagta cttcaactac 1500 tctgatgcag acgacatcct gagagaaata aacagaatca ttccgcccct tgctggcgcg 1560 acacccgaga ggctcaagaa gaacctcaaa ggctgtatga taccctgccc agacgagaac 1620 actgaggttc cgaggctctt tgtccagggc ttcctcacgc caaacggaaa ggcccagctt 1680 attcctgtgg agtataaaga gcctggagaa gtccccgatg aggagtaccc gttctggctc 1740 accaactaca ggttcgttgg ccacttccac accggaacca tgagccacag gagcaagagc 1800 ctgagcaaga ggtggccaga ggagtacatt gagatcaacg agaacgacgc gaagaggctc 1860 ggcataaagg acggcgacct cgtgagagtc gagaccagga gggcagcgct ggttctcagg 1920 gccaaggtta caccgcacat cagggagggc gtcgttgccg cgccgtggca ctgggacttc 1980 aactacctga ccacggacgt cctcgacgaa tacgccaaga tgccggagtt gaagacggcc 2040 gcgtgtagga tctccaaggt tgaggggtga 2070 <139> <211> 552 <212> DNA <213> Thermococcus onnurineus NA1 <400> 139 atggacacta aggaacggat attgaaagct gcagaagaac tctttgccga gaagggctac 60 gataagacca ctgttgatga aatcgttgag aaagctagag ttgctaaggg cactttctac 120 aactacttta agagtaaaga ggaattgata aaaatcgttg ctctccagtc tctgccgtac 180 tcagcaatac gagaggagct tgaaaaagag catgagaacc tcgctgattt tctgcacagt 240 attgcaaagg cgtacatcgt atattactgc gatcctgttc tgagatccct gtttttctac 300 acactagctg ttaagggtaa gataagagag gttgaggaaa ttcacaggaa attctgcaca 360 gaggcgattt ccaaaggtgc caagaagata tcacagctcg caaacgtgga tgaaaaaact 420 gccatggttg tttttaaggc gttttatgga gccttatttt cgagacttgt ttttgctgag 480 tattcgtgta cccctgatgt tgattatgtt tcggaactca tccagcttat agagaaaagc 540 ttgaaaaact ag 552 <210> 140 <211> 246 <212> DNA <213> Thermococcus onnurineus NA1 <400> 140 atgtgtctgg cagttccagc taaggttctt gagatcaacg gaaacgttgc gattgttgat 60 ttcgggggcg taaagaggga agctcgcctt gatttgcttc ctgatgttaa ggttggcgat 120 tacgtgatcg tgcatacagg ctttgcaata gagaagctgg acgaggagcg cgctaaagag 180 atactcgaag catgggacga ggtgttcaga ataactcggg gcgaggaact cccacggggg 240 tattga 246 <210> 141 <211> 357 <212> DNA <213> Thermococcus onnurineus NA1 <400> 141 atgctcgaga agttcaggga tcgaggactg gcccagaaaa tcgttaaaaa aatccacgaa 60 gaggcaaatg gtctcgaaga ggtccgtttc atgcacgtct gcggaaccca cgaggacact 120 gtaacgagaa gcggaatccg gtcactactt ccagagaatg tcaaaatcgt cagtggcccc 180 ggctgtcccg tctgcataac gccagtcgaa gacatagtca agatgcaaga gataatgcgc 240 caagctcatg aagaaggcga gagaataatt ctcacgacat ttggggacat gtacaagata 300 ccaaccccga gaggaagctt cgccgaccta agagcgaagg ctatgacgtt aggatag 357 <210> 142 <211> 693 <212> DNA <213> Thermococcus onnurineus NA1 <400> 142 gtggttcact ttagtccagg ttttgaaacc accactgcac cggccgctgg gatgctcaac 60 tctgttgttg aggaagggct tgagaacttc aaaatatatt ccgttcaccg cctaacgccc 120 cccgcagttg aaactctagt gaagcagggc acaaagttcc acggattaat agatcccggc 180 cacgtctcaa cgataattgg ggtcaaggga tgggaatata taaccaccga gtatggcatc 240 ccgcaggtta tagctggttt cgaaccagtg gatatgttac ttgcgattct gctgctcctt 300 aagatggtca aagctggtga agcaagaatc atcaacgagt acacccgggc tgtcagatac 360 gagggcaatg ttacagccca gcggcttgtg gacaagttct ttgaggttaa agacgccaga 420 tggcgtgctc taggtgtgat gcccaagagc ggactcgaac tcaagaagga gtggaaagag 480 ctggagataa ggacatacta tgatccagaa gtcccgaagc ttcccgacct tgaaaagggt 540 tgtctttgcg gtgccattct gcgtggattg gcactccctc cacagtgtcc acactttggg 600 aagacctgca ctccaagaca gccgataggg ccatgcatgg tgtcttatga ggggacatgc 660 agcatctttt acaaatatgg ggctttgttc tga 693 <210> 143 <211> 2442 <212> DNA <213> Thermococcus onnurineus NA1 <400> 143 atgcagcatc ttttacaaat atggggcttt gttctgacac caaaggttca aaactccttc 60 caagttttta acttttggtt tcagaaacct ataaagactg taaaaacaaa gaagaaagcg 120 gtgagaggaa tgaaggctta cagacttcac gttcagggca tcgttcaagc ggttggattc 180 cgaccgttcg tttacaggat agctcatgag catggtttaa ggggctacgt caaaaatctc 240 ggcgatgcag gtgttgagat cgtcgttgag ggtcctgaga aggatatagg tgcgtttctg 300 ctagatctga gagagaagct ccccccttta gctaagatcg agaagattaa aaagagggag 360 attccgattc agggctttga ccgcttctac atcgagaaga gctcccaagg gggaagcgga 420 ggagactcga taatcccgcc ggacatagcc atatgtgagg attgtctaag ggagcttttc 480 gacccaacca acaagcgcta tatgtaccca ttcatcgtct gcactaactg cggcccgagg 540 ttcacaatta ttgaggattt accttatgat cgcgtgaaca caacaatgag agagtttccg 600 atgtgcgact tctgcgagag tgagtacaaa gatcccttga acaggcgcta ccacgccgag 660 cccgtctgct gcccggtctg cgggccgagc taccggttat ataccaacga cggtgaggag 720 ctcataggcg acccgctgaa aaaggccgcg gagctgatag ataatggcta catcgtggcc 780 atcaagggga tcggtggaat acatttagcc tgcgacgcga cgagggaaga tgtggttgcc 840 gagctaagga agagaatcca caggccacag aagcccttcg caataatggc gaaggacgtg 900 gaaactatag aggagttcgc cttcatatcc ccggaagagc tggaggaact gaagagttac 960 agaagaccca tcatgacgct ccgcaagaag gagcccttcc cgcttccgga aaacctcgct 1020 cccggcttgc acaccatcgg tgtgatgctc ccctatgccg gcacccacta catcctcttc 1080 cactactcca agactcctgt ttacgtcatg acctccgcca actatccggg catgccaatg 1140 gtcaaggaca acgagagggc ctttgaggag ctgaaggacg ttgccgacta cttcctcctc 1200 cacaacagga agatactcaa cagggccgac gacagcgttg tcaggttcgt caacgggagg 1260 agggcggtta taaggcgctc ccgcggcttc gtcccgcttc caatagagat tcccttcgac 1320 tacagaggat tggcggtcgg tgcagagctc atgaacgcct tcggagtgac caaaaacggt 1380 aagatttatc cgagtcagta cataggaaat acgtcgaaag tggaagtcct tgagttcatg 1440 cgcgaggcgg tggagcactt caagaggatt ctcagggtta gggacttcga tttaatcgtg 1500 gccgatctcc atccaggcta caacaccacc aagctcgcca tggaaatggc caacgaactc 1560 ggcgtcgagt tcctgcaggt ccagcaccac tacgcccaca tagcctcgat catggcggag 1620 cacaaccttg acgaaatagt cggcatagcg gtcgatggag tcggatacgg aacggacggg 1680 cacacgtggg gcggcgaggt gatctacctc tcctacgagg atgttgaaag gctggcgcac 1740 atagattact atccacttcc cggcggtgat ttggcgagct actacccact gagggctcta 1800 atgggaattt taagcaaggt atacggcata gaggagctgg aagaggtaat agagaagtgc 1860 tgtccaaagg ccatcgaaag cctccgctac ggaaaggttg aattcaacgt cgtcctcacc 1920 cagctggcga aagaggtaaa cacgagttac gcatcatcta cgggcagggt gctcgatgcc 1980 ttctccgttc tgctcaatgt agcctacaga aggcactacg aaggcgagcc ggcaatgaag 2040 cttgaaagct tcgccattcg cggcaagaac gacctcggct tcagcgttcc cgtggagggc 2100 gagctgataa aggtagaaga gctcttccag caggcactag agatcaatgc ctctcctgcg 2160 gacgtggcct attcagtcca cctggccctc ggaagggcat tcgcagagat agcagtggag 2220 aaagccaagg aattcggcgt caagaacgta ggaatcagcg gcggtgttgc gttcaacgaa 2280 cttatcgtga agacggtgag gaagatagtg gaggccaatg gtctgagatt ctactcgacc 2340 tacgaggtgc cgaggggcga caacggaata aacgtcggtc aagccttcct cggtggactt 2400 tacctggagg gatatctaac gaaggaagac ctgatgctgt ga 2442 <210> 144 <211> 1011 <212> DNA <213> Thermococcus onnurineus NA1 <400> 144 atgaaaatca agctcgaaca cggagccggt ggagaaataa tggaggagct tctgagagac 60 gtcatattga agaacctcag cctgaaatct gcgggtggaa ttggcttgga tgccctcgat 120 gacggggcca caataccctt cggggataaa cacatcgttt ttaccatcga cggccacacc 180 gtgaagcccc tcttctttcc ggggggagac atagggcgac tggcagttag tggaacggtg 240 aacgatttgg ccgttatggg cgcaaagccc ctcgcgctgg cgaactcaat gataatcggg 300 gagggcttcg atggggaaga cctgaaaaaa atcctccgtt cgatggacaa aaccgccagt 360 gaagtgcccg ttccaatagt cacaggcgac acgaaggtcg tcgaggatga gataggcatg 420 ttcgtcataa cggcaggcat aggaatcgcg gagagaccta taagcgacgc tggggcgaag 480 gtcggcgacg ccgtcctcat cagcgggacg ataggcgacc acggaatagc actgatgagc 540 catcgtgaag ggataagctt cgagaccgag cttaagagcg acgtggcccc aatctgggag 600 gtcgttgagg ccgttgcaaa ggccatcggt tgggagaaca tccacgccat gaaggacccc 660 acgaggggtg gtttaagcaa cgccctcaac gagatggcca ggaaggcaaa cgttgggata 720 ctgataaggg aagccgacgt gccgataagg cccgaagtca gagctgcaag tgagatgctc 780 ggcataagcc ccttcgatgt agcaaatgag ggcaaggtcg tcatgatagt tccaaaagag 840 cacgccgaag aagccctaga ggctatgagg aaaacggaga aaggaaagaa cgcggcgata 900 atcggtgagg ttatagagga ctaccgtgga aaggtcttag ttgagacagg cataggtgga 960 aagagattcc ttgagccacc tgcgggagac cctgttccga gggtctgctg a 1011 <210> 145 <211> 294 <212> DNA <213> Thermococcus onnurineus NA1 <400> 145 atgataattg cgaagccatg cgtaaccatg aagggaatag tgataagcgg ctattcttgg 60 gatagaaagg tcgaggtgga tctaacgaga accgcgcagt gcctgaagga aaaaggttac 120 gtcatcaaaa agattctccc cgggatgatg ctgatccttg agatggaagg ctatgaggtc 180 agcgtctatc caagcggtaa gataatcatc aagctccttg aggactcgaa aaagggtgag 240 gagatggcta gaatcatata cgactgcgcc ggaatattgg aggtggtttc atga 294 <210> 146 <211> 1200 <212> DNA <213> Thermococcus onnurineus NA1 <400> 146 atgaggattc cggaggatgt taggaaggac atacctctta caaatgaggt gatttacttc 60 gacaacaccg ccacttcact cacgccgaag ccagttgttg aggccatgga cgagtactac 120 ctgaagtacc gcgccaacgt tcacagaggc gttcacaggc tctcccagat ggcgactcac 180 aagtatgagg agtccagaaa gatcgttgcg gacttcattg gagccaagtt cgaagaaata 240 gtcttcacaa agaacacgag cgagagcctt aatttagttg cccttggcct ggggcacata 300 ttcaagaggg gcgacaaaat cgtgactacc ccctacgagc accactccga tctgctcccc 360 tggcagcgtc tggccacgaa actcggcctt aagctggagt tcatagaagg tgacgacgaa 420 ggcaaccttg atttaagcga tgcggaaaag aagataaaag gagcaaagct tgtcgctgtt 480 cagcacgtct cgaacgcgct gggagtgatc catgaggtgg aagagcttgg aaagatcgcc 540 aaggatgaag gcgcgatatt cgtagtcgac gccgcccaga gcgccggcca tatggaggtt 600 aacgttaaga agctccacgc ggactttttg gctttctccg gccacaaggg acccatggga 660 ccgactggaa taggtgtgct ctacatcagg gaagagttct ttgatacatt cgaaccgccc 720 ctcataggcg gtgggactat tgaggacgtt agtctagacg gctataagct cacagagccg 780 ccagagcgct tcgaggccgg gacgccgaac ataggtggtg ctataggcct cgcagcagga 840 atccgctaca tcgagaggat tgggctgggc agaatcgaga ggcaggagca caagcttgtt 900 aagagaacca ccgaaggcct cgacgagctc gaggtcccgt ggtatggacc gaggaacctg 960 aagaaacatg caggagtcgt aagcttcaat gtgcctgggc ttcaccccca cgacgtcgct 1020 gctatactcg atgaccacag cataatggtc cgctccggcc accactgtgc tttgccggtg 1080 atgaaaaagc ttggaataaa cgggacggtg agggcttcat tccatgtcta caacagcctc 1140 gaagaggtcg aaactttcct cggagtcatg gaggagctgg taaaaggtct gaagacttaa 1200                                                                         1200 <210> 147 <211> 699 <212> DNA <213> Thermococcus onnurineus NA1 <400> 147 atggaggttg attttaaggt cgccccagag gagaaagtca ggaagaagcc atcattcatc 60 aagccctgga tgggcctcaa gtacctcttc aagaagcccg ttactatcaa gataccctac 120 gagagggtac agatagctaa ggactacagg ggattccaca ccctagactg gaagaagtgt 180 gtcggctgta acttctgcgg ccagatatgt ccggcgaggg caatagagat gacctggata 240 gaagtggatg gcaagatgga gaagaggcca catccaaaga tagactatgg caggtgtacc 300 ttctgtgagt tctgtgtcga cgtctgtcca cctggagcgc tgggcttcat cgagaactac 360 atcctcacca ccgagtggaa ggacgaggag ctggagctct ttgactgggt tccaatccat 420 ccagacaagt tcagggagat aaacgagaag ttccccgact acagattccc ggtggagaag 480 atagagttca acaaggaaac gaaggaggtc acctactacc tgagagacgg agaggtcatg 540 aagttcaaga tactcggcta cggcatcaga ccgccgaagc caccgacaaa gcctgctcag 600 aaggcagccg caaaagcagc ggaaaagaat gataccaagc ctgttgaaaa gcccactgag 660 aagaaggaag ctgggaagat agaagaaaag aaagaatga 699 <210> 148 <211> 1176 <212> DNA <213> Thermococcus onnurineus NA1 <400> 148 atggtttcac agaatgagct cattcgggaa gcgagagaaa atgggatgga tctgctccca 60 atcgataagg acacttacga gttgttcttt ggcccacagc acatggctac tgagaacttc 120 agcataatcc tcaagatgga cggtcacagg gttgtgaagg ctatagccaa ccccggcttc 180 ctccacaggg gctttgagaa gctcgccgag tacaggccgt ggcacacgaa catagcgctc 240 ctccttagaa tctgtgttcc agagccagac gtccccgagg caatatactc aatggccgtt 300 gatgagataa tcggctggga ggttccagag agggctcagt ggattagaac aaccgtcctc 360 gaaatggcga gggtttccgc atatctgttc tggataatgg gtctcagctt caagctcggt 420 gtctacactg ccggtcagtg ggctgctgcc tacagggaga ggctgatggc cctcttcgag 480 caactgaccg gtgccagggt ctatcacata tacaccatcc ccggcggtgt caggagggac 540 attccgggcg acaagtggct tcgccagctc aaggacaccg tcgagtacat caggagcaag 600 ttatcagact tcgacaacct tgtcttcgag aactacgttg cccacaggag gcttgaggga 660 attggagtga tggacaagaa gtttgccctc gccgaaggcg tcactgggcc aaacctcaga 720 gccaccggcg ttccctacga cgtgaggagg gcagatccat acctgctcta tccagagctc 780 gacttcgagg ttcccgtcct gaaggagggc gatgccctcg cgagggcttt gataaggcgc 840 ttcgagcttg agcaggatct ttacatcctc gaccagctcc tcgagatggg accgccgagc 900 ggaccgtata aggttgaaga tcccaagctc aagaatctcc cgaggtttaa ggttccggct 960 ggagatgcat ttgcccacgt ggaatcaacg aagggcgact ttggtgccta tgtcgtcagt 1020 gatggaaagc acaagccgta cagagtgcag ataaggggcc caagtatagc ccacggagtc 1080 agggttctcg agcagctctt ggttggagca agaatagccg acgtccccgt gatattgatg 1140 agccttgaca actgtccacc agacattgac aggtga 1176 <210> 149 <211> 534 <212> DNA <213> Thermococcus onnurineus NA1 <400> 149 atgggggaag ttaagtggga gagagagcag atgctcgttg acaaaatcct tgaaaaagcc 60 ccctacgccg agggcaaggt gcggcgcgaa cggaggattg agttcagcat tccggcagac 120 aggataaggg acttcctcat gctgctcagg gataacgact tcgagctcat gctccagata 180 acgaccgtcg actggcccaa cgacggtgag cttgagctta tctatcagat gtggagcgtg 240 acccacagaa cccacgccat ggtcagaaca cggattccga gggatctcga taaggcaagg 300 atgccaaccg tcaaggatat ctaccctgtg gctgagacct acgagaggga cgcccacgac 360 ttctttggag tctacttcga gggcaatgag aagatggaga tgccgtggat cctcgacgat 420 accgagcagg ggctcttccc gcacagaaag gacttcgaca tgctgaccta cgtgaagaag 480 aagtacaagc tgctcgacag gttcgatgag gataaggaca actacgtgat ttga 534 <210> 150 <211> 573 <212> DNA <213> Thermococcus onnurineus NA1 <400> 150 atggtggact ggagactctt tgaacctctc tttaattatg cgagaaagaa gagcctttgg 60 attgtgtcgt tctgtaccgg atgcggcggt atagagatgc cgcccctcat gacgtcaagg 120 tatgacctag agcgattcgg tatgataccg gacccgagtc caagacagta cgacctcttc 180 ctcatcacgg gctacgttac gccaaagacc ctcaaaagaa tcataatcac ctacgaaatg 240 gcacctgatc caaagtacgt cctggcgcac ggctcctgcc cgctcaacgg tggaatctac 300 tgggacgcct acaacgctat caagcacctc gacaagtaca tcccggtcga tgtcgtcata 360 gccggatgca tgccgcggcc agaggcagtc atggacggaa tccagaagat aatggagatg 420 attgagaacg gaacagcaga cggatggaag aggtacaagg agaactacga gtggtacaag 480 aagaaccagg acgagctctt cggcgaagga tggcgtgaga gggaagccag aaggtggatc 540 ccatggctgg tggacaagaa aaaggaggag tga 573 <210> 151 <211> 930 <212> DNA <213> Thermococcus onnurineus NA1 <400> 151 atgcttgagg tagcgctgaa ggctttattc ctcctaattt atgcgacctt cgtcgggttc 60 atgttcatgg gaataatcag gaaagtgagc gcaaggatac acagaagggt aggaccgcca 120 atctatcagc ccatacttga caccatcaag ttcttcggca agaaggagaa cattacccac 180 ggattaatct acgacttcgg catcatctac gcccttggag ctacggtact ggctttgttc 240 ttcatcccgc tgggaagcgt cagcatactc agggcctacg gggacctcat cctcatcacc 300 ttcctcctcg agataccgat gctgggaata atgttcgctg caatgagctc aggaaacccc 360 tatgcaggaa taggcgccca gagggccctg cttactatgc tggccattca ggtgcccctc 420 ggcttcgcga taatagcact cgcagagttt tacggaacct tcagcaccta cgagatagtc 480 atggctcagc agatcagcgg atggagtata atacacctgc cgctcctctt agccgccata 540 gcctacgaca tcgtccttca ggcaatgttc ggcaaggagc ccttcgacat catgattgca 600 ccaggtgaaa tctccctcgg tccgatggtc gagttcggcg gaaaacacat ggggatactc 660 cagatacagc acgccatagc cctcttcgcg gagacgctgt tcttcagcaa catattccta 720 ggcggagcag ttattactgc ctttgcaagc ccgatactga acaccatagc cagccttgca 780 atactcctcg tcaagcagat agcggtgctc ctcatagcgg tattcgtcag cacgatattc 840 ccgaggttca cgattgacca ggcggccaag ttctactgga agtggccgac catcatagcc 900 gcactcggag cgatacttgc aagcctgtga 930 <210> 152 <211> 1863 <212> DNA <213> Thermococcus onnurineus NA1 <400> 152 atgattaacg aactcatcgt tatcattttc gcgcctctga ttgctggcgt tatcgcctgg 60 gcgcttgaca tcaaagttat tagagagctg ctcggcgttg ccggtgcagc ccttccccta 120 gcttacctca tcaagctcta tccaaccgtt gatgccgaag gaatgataca gcacacgctg 180 aactttgccg gcttcaccct tgagttcaca ttaacccaca tgagctggat attcgctatg 240 atagccggtg ttgttggcct tgccgcagtg cttggaatgg tctcaacctc aaagaacggc 300 tacgagtggc tgtttgccct gatgagcctc accggcgtct acggcatctt catagcctac 360 gacctgttag ggttcttcat cttctgggaa atcatgacct tcggaagctt catgatggtc 420 ctcaagtaca acagggaggc ctcactcaag tacttcgtgc tcagcgttat cggagcctac 480 gcgatgctca tagcgatagg cataatctac gccaaagtcg gctccttcag ctttatagaa 540 gtttacagag cgttctcaca ggacgcggca ctggcggctg tcggctcatc taccatcttt 600 agcaagggcg aaattgcgtt aatcttcggt ctcttcctgg tcgcctttgg cgttaaggcc 660 gggatgttcc cgcttcacgt gtgggcgccc gatgcctaca gcgagaccaa ccagagctac 720 accgcgatgt tcagcggcgt cctcagcaag actggcgttt acggatttat cctcatctac 780 atgctcatag gctaccgcct gctctacgaa ttcggaacga ttagaaccgt ccccaagttc 840 ggctacatca tagcatttct cggtggtcta accattatcg tcggcggcat cctcgcggcg 900 ctccaggagg acatcaggaa gctcttcgcc tactccagta taagccagat aggttacatc 960 ctagtgggca taggtgttgg gactgcattg agcatcgaag ccgccatcta ccacgccata 1020 agccacgccc tcttcaaggg actgttcttc ctcatagtgg ccaccataat ctaccgcacc 1080 gggaagacgg agttcaaaga catgggcgga ctggcagaga agatgccagt aacatttgcg 1140 atggcctttg tagcaatact cagcctcgct ggaattccgc cgatggtcgg cttcgccagc 1200 aagtggctaa tctttgagtc ggtcataagc cagaacatgc caatactcgg tgcgatggtg 1260 ttctttggaa gtgccgtagg tttcgtctac ctcatcaggt tcacctacgc agtctggttc 1320 ggtcagaggc caaccgacct tgatgacacc aaggacgctc cattgcccct tgccataggc 1380 atgggcatac ttgcggtgct caacgtcgtc ttcggtgtcg ctccaggtct tgtggcccag 1440 gagttaaaca agatattcgg cagagaggtc atcggtggaa ctatatggga gctgaacctc 1500 ggcttcggta agtacaacgg cctcctcctc accgtctggt ttgtggtcgg catgctcatt 1560 gcggccatag tctacttcat gggggcaggc gtgagaaaag tccccgttac ggacacctac 1620 cagtcaggaa acccagtaac gatggagtac aacctcacca tcaggaggaa cttcttcctt 1680 ccgctcaagg aggccctgtc cttctggctc agaatgagct ttgacaggtt ctacaaggac 1740 gttggaaaga ctgttgagga cttcgccgag accatgagga actacgtcta caatggaaac 1800 gtccagagct atgcctggta tcttgccata gtcctgttaa tcctcgctat gtggggggtg 1860 tga 1863 <210> 153 <211> 1488 <212> DNA <213> Thermococcus onnurineus NA1 <400> 153 atgaacgggc agtacgctgc tctgctcata gcgttacccc tcatcagcgc gttcttcgtc 60 cccgtgttta agggaatggg aaagaacgcc gtcaggtact tcctgatcct agttaccgcc 120 ctccagactg gaatagccgc atgggtcttc aaggaggtct atactactgg ccagccaata 180 atagtcatcg ccggcggctg gaagccaccc gttggaatca acctctacct cggccatttc 240 gcggcgctct tcgtactcat aatagcagtc gttagcctct tcacttcgat cttcagcatc 300 agcgcggtca aaaccgaacc catcgacaag tatgccatgc tcttcctcct gctgatgctt 360 ggtgccaccg gaatgatagc gaccggtgat atattcaacc tcttcgtctt catggagata 420 acggcaataa ctgcctacgc cctcacggcc tacaacaaga caggtgaatc ggccgaagcg 480 tcaatgaagt acatcatcct cggtggaatc ggttcaagct tcttcctcat cggtgtcgct 540 ttaatctacg gctccctcgg aaccctcaac atggcccaga tagcccagct cgcagcaact 600 atggatccca ccgtagccca ggtcggcctc gcgctcataa ttcttggtct ggccgtcgaa 660 gctgagctct tcccgctcaa cgcctgggcg cccgacgctt atcaggcagc accgcaccca 720 atcaccgtca tgttctccgc ctttgtcgtc aaggctgccc tctacgccat ggcgaggatt 780 atctacctca tgcaggtcgt cgagagctgg ggctcagtgc tcaggcttct catcgtaatg 840 gccgccctca ccgtcgtcgt tgccgagctg gcagcgctta ggcagaagaa cgtcaagagg 900 atgatagcct actccagtat cgcccagata ggcttcatag cactcgcctt cgccctcggc 960 actcaggccg gcgttgacgc tggtgtcttc cacatgatca accacgcaat agtcaaagtt 1020 ctcctgttcc tcgccgtcgg ctacgtggcg ataaccctcg gcggtgcaga gctggagaag 1080 ttccagggac tcggcaggag gatgccgctc acggccttcg ctatctcagt tggtgcagta 1140 gccaccatcg gaataccgct cttcaacgtc ttctggagca agatcagaat aatccttgcg 1200 accctgaacg tcggctacac ctgggcggca gtacttgtac tttcagcgag cgtcgtggag 1260 gcggtctatt acttcaggct gctccacgcc atatggttcg aaggagaggg agagagaatc 1320 agcgagagca tggtcattgg cctcatgatg ttcttcctcg cggctttggt ggtcgtcata 1380 ggagtctacc cggactacgt ttgggagctc gccaggaagg ctggggagga catcttcaac 1440 gtggcccagt acgttaagaa cgttccactg atgggggtgg gagcatga 1488 <210> 154 <211> 345 <212> DNA <213> Thermococcus onnurineus NA1 <400> 154 atgataagcg tctactactt tggtgcgata gcgctcatcc tcgtaggcct ctacgcaatc 60 ctggtgaaaa agaacctgct caagattctc ataggcctca gcataatgga aacgggtgtt 120 aacctgctac tcatcagcat tggctacgtg agcggcaaga gcgccccaat actgagcgaa 180 ggaataggtc cgagccaggc agttgacccg attccgcagg cattggttct cacggctata 240 gttattggag ttgccaccac cgccatggcc cttagcgttg ccatactgat ttacgagaag 300 tacggaaccc ttaacgttga agagataagg aggttgagag gatga 345 <210> 155 <211> 711 <212> DNA <213> Thermococcus onnurineus NA1 <400> 155 atggtagtca agcgcgcttt ggcaataatc acactcctca tcatagggta ctggctggct 60 caggggttgg ccggggtccc cttcggcgag gacaagatgc tcgtcggcca gtactatctc 120 gacaatgtga aacagcagac cggctctgtt aacgccgtca ccgccgtcgt cgtcaactac 180 cgcggatttg atacccttgg tgaggtcacc gtcctgttta tagcatctac tggcgttgga 240 gccctgctct ggagaagaaa gaaggagaga accgcaaaga ccgagggctc aatagttctc 300 acaaccggaa cgaggctgct cttccccttc gtgatgctct tcggtgccta catcttcatt 360 cacggacacc tcaccccggg tggaggattc ccaggaggag ccaccattgc cactgccttc 420 ctgctgctgt acctcgcgtt cataacctac gaaatcccac acaggggctt tgaggtcact 480 gagggcctag ctggcatgag ctacgtcgcc gttggtctag ttggtctcgc aataggcggt 540 tacttcctct tcgactggat atggcagacc tggcagtttg gctgggacaa cgtcggtaga 600 ctgctgagcg gtggcttcat accaataatc tacaccatca tcggcattaa ggtcggcacg 660 gagctcagcg gaatcatcga caacatgctc aaggaggagg tgagcgaatg a 711 <210> 156 <211> 285 <212> DNA <213> Thermococcus onnurineus NA1 <400> 156 atgaacctcg cgtattacat cccccactta atagttgcga tcatggtagt ctccgcaata 60 ctcgccgtcg agtggaagga catactagct gccgccgtcg gaatggcagc cgtcagcctg 120 ttcgcctcac tgctgttctt cttcctgcag gctccggacg tggctatgac cgaggcagca 180 ataggtgctg cactcagcgg tgccgtgttc atattcgcca ttaagagaac ccagaggttc 240 gagacggagg aagaagaaac gcccgggtgg tgggtgagat ggtag 285 <210> 157 <211> 411 <212> DNA <213> Thermococcus onnurineus NA1 <400> 157 atgaacgctc ttacggtgat cggagaagtt ctcgtcctgc tggggacgtt cttctacttc 60 ctatcagccc taggtctgat caggatgccc gacgtttaca acaggatgca gacctcgacc 120 aaaagcgcca cccttggttc tctgggtgtc atagttggtg tcggaatttg ggctctcggg 180 accgacttcg gaagcgcggc atggctcaca aagaccattg tcatcgctgt gttcctcctg 240 cttaccaacc caataagcgc ccacgcactc ataagggcag cgtacaagag cggaatccca 300 ctgtgggaag gcagcatcgt cgacaagtac agtgagcacc tcgaggcgaa ggtcaaagcc 360 gaggaaaccc ccgaagaaac tgatgaaacc ggagagggtg gtgaggaatg a 411 <210> 158 <211> 255 <212> DNA <213> Thermococcus onnurineus NA1 <400> 158 atgataggga taaacgttta tctcgccctg atagcgatag caacgctcct cagcatgtac 60 aggatcttca gaggaccaac gacagtggac agaatcgtgg ctgttgatat aatgacaacc 120 ataaccaccg gactaatggt gctcttcgcg ctctactacc agagaatgat attcctcgac 180 gttgccctgg tatacgcgat tctggccttc ggtggagtca tggcattcgc gcgctacatg 240 gagggaggcc tatga 255 <210> 159 <211> 507 <212> DNA <213> Thermococcus onnurineus NA1 <400> 159 atgggagaag caagcaagat aagccgctac ctgtacacgg ttatcgtgct gttcctgata 60 tggctgtttc taacggctag tttagatcca caagaactcg gattcggcct gctactgtct 120 ctaatcgtcg gagccttcac ctacgagata ttcaccacca atggcctcgc aaacctgcat 180 ccaaagagga tcgcctacgc aatagcttac ataccctact tcctctgggc aatgatcatg 240 gccaacctcg atgttgcata cagagttctt caccccaaga gacccataaa cccaggaatt 300 gttgagtgca gaactgttct caagagcgac gttgggaagc tcagccttgc gaactcaata 360 accctgacgc ccggaaccat aaccctcgac gttgacggtg acaggtactt catccactgg 420 atagacgtca aggatgattc cgttgaaggc gcttctaaga gcataaccga gccctttgaa 480 aagttcctga aggtgatctt cggatga 507 <210> 160 <211> 450 <212> DNA <213> Thermococcus onnurineus NA1 <400> 160 atggagaccc taatcctcgc cctcggcaac gaacttatga aggatgatgg tgtcggcctg 60 aaagtcggca ggcttttggc ggaaaaaggt tacaacgttc tagaagttgg cacagacata 120 ttcatgctcc agagccatta cagtggagag gaaaggctca taatcatcga cgccatactt 180 agcgaaaagt tcaagccggg agaaataatc cacgtgagcg gcggtgaagt tttcgagaag 240 ctgaaagctg aaattaggag tgcgcacttc atgggggcaa tagacgggct caaactcctc 300 atggcactcg atgaaaggct tgccaacgtt gaaatccact tcattggtat tgttgctaag 360 gaaatcaacc tgggtatgga actgaccgag gaagttaggg aagcccttcc gaaggctgtt 420 gagctcgttg aagaattagt caaaaaataa 450 <210> 161 <211> 1290 <212> DNA <213> Thermococcus onnurineus NA1 <400> 161 atgaagaacc tctaccttcc aatcaccgtt gatcacatag cgcgtgtaga gggcaagggc 60 ggcgttgaaa tagtcgttgg tgatgacggg gtcaaggagg tcaagctcaa catcattgag 120 ggtccgaggt tctttgaggc gataaccata ggcaagaagc tcgacgaggc tttggccgtt 180 tatcctagga tatgctcctt ctgttcagca gcccacaagc tcactgccgt cgaggcggct 240 gaaaaggcag ttggctttga ggtacgcgag gagatacagg ccctcaggga ggttctctac 300 attggagaca tgatagagag ccacgcgctc cacctctacc tcctcgtcct gccagattac 360 atgggctact ccaacccgct caaaatgctt gacaagtaca agaaagagat caacattgcc 420 cttgacctta agaacctcgg aagctggatg atggacgagc ttggtgcaag ggccatccac 480 caggaaaacg tcgtcatggg tggttttggc aagctccccg acaagactac gctggagaag 540 atgaagaaga ggctccagga ggcccttcca cttgcagaat acaccttcga gctgttctcc 600 aagcttgagc agtacgagga agtcgagggg ccgataatcc atatggccgt caggccgagg 660 ggagacgtct acggcatata cggcgacgcc ataagcgtca gcgacggttt cgagttcccg 720 agcgagggct acaagaagca catggttgag cgcgttgtgg agcacagctt cgccaagcac 780 agcttctaca acggggagaa gcccttcatg acgggagcca tatcgcgcgt tgtcaaccat 840 gctgataagc tctacggaag ggcaaaggag ctctacgaga gccacaagga cttactcagg 900 ccaaccaacc ccttcgccaa caacctcgcc caggcgcttg agttagtgta cttcatggag 960 aggggcatcg accttatcga cgaggccctc gctaaatggc cgataaggcc gagggacgag 1020 gtagatgtaa aggacggctt cggtgtcagc accaccgagg caccgcgtgg aatcctcgtc 1080 tacgccctag aagtcaagga cggaagagta gcttacgcgg atatcataac gcccacagcc 1140 ttcaatctcg ccatgatgga ggttcatgta cgcatgatgg ctgagaagca ctacaacgac 1200 gatcctgaga ggctgaagct ccttgctgag atggtcgtca gggcctacga cccgtgcatc 1260 tcctgttcgg tgcacgtggc gaggctttag 1290 <210> 162 <211> 798 <212> DNA <213> Thermococcus onnurineus NA1 <400> 162 atggagggga aggttcgtat agggttttac gctctcacct catgctacgg ctgtcagctc 60 cggttcgcca tgatggacga gatacttcag ctgctcccga acgctgaaat cgtctgctgg 120 tacatgcttg accgtgacag cagtgaagat gagcccgtcg atatagcctt catcgaagga 180 agcgtttcaa cagaggaaga ggttgaactc gtcaagaaga tacgcgagaa cgccaagata 240 gtcgttgcag ttggggcgtg tgcaacccag ggtggggttc agagctggga gaaggataag 300 agcctcgaag agctatggaa ggccgtttac ggcgatggga aggttaagtt cgagccgaag 360 atggccgaac ccctcgaaaa ctacatcaaa gttgactacc gcatctacgg ttgcccacct 420 gagaagaagg acttcatcta tgccataggt accttcctcg ttggttcttg gccagaggac 480 atcgattacc cggtctgtct tgagtgcaga ctgaagggta acacatgcat tctcatcgag 540 aagggcgagc cgtgccttgg cccgataacg agagcgggct gtgacgcaag atgcccgagc 600 tacggaatag catgcatagg atgtagaggg gcgataggct acgacgttgc atggttcgac 660 tcacttgcca ggacgttcaa ggaaaagggc ctcaccaagg aagaaatcct cgaaagaatg 720 aagattttca acgcacacaa tccaaagctg gaggagatgg tcgataaggt ctttcagttt 780 cagggggtga aagaatga 798 <210> 163 <211> 876 <212> DNA <213> Thermococcus onnurineus NA1 <400> 163 atgaacgagg ctcacgtctg catgtgtcac gacaatccct acgcccttga cagggtcaag 60 gttctcagag tgtaccgttt aacagaaaca gagaagctat ttctgttcag gtttgaagat 120 caagagatag ccgagaactg gacctttaag ccaggacagt tcgttcagct taccataccc 180 ggtgttggcg aagtcccgat aagcatctgc tcgtctccca tgaagagagg attctttgag 240 ctctgtatca gaaaggcggg aagagtcacc acagttgttc acaagctcaa gccgggcgac 300 actgtccttg tccgcgggcc gtacggaaac ggcttccctg tcgatgagtg ggaagggatg 360 gacctactcc tcatcgccgc aggattagga acagccccac tcaggagcgt cttcctctac 420 gccatggaca accgctggaa gtacggaaac ataaccttca tcaacaccgc ccgttacgga 480 aaggacctcc tcttctacaa ggaacttgaa gccatgaagg atctcgcaga ggccgagaac 540 gtccagataa ttcagagcgt aaccagagat cctgactggc ccggcagaca tggaaggcct 600 cagaagttca tagtcgaggc caataccaac ccgaagaaca ccgcgatagc tatctgcggt 660 ccgccgagga tgtataaggc ggtcttcgag gcgctcatca attacggtta ccgccctgag 720 aacatctatg taacgctgga aaggaaaatg aagtgtggaa taggcaagtg cggccactgt 780 aacgtcggaa cgagtactag ctggaagtac gtctgcagag atggaccggt tttcacgtac 840 ttcgacatag tatcaacgcc gggactgctg gactga 876 <210> 164 <211> 1104 <212> DNA <213> Thermococcus onnurineus NA1 <400> 164 atgagatacg tcaagcttcc aaaagaaaat acatatgaat ttttagaaag actaaaaaac 60 ctcggaaagc tgtacgcccc agtgaaaatt tccgatcagt tctatgattt tagagaaatt 120 gacgacgtca gaaagattga attcaactac acgagaaccc tgatgccgcc gaagaagttc 180 ttcttcgcac caagggagaa gatgttcgag ttcagcatct caaaagcaga gtatagggaa 240 gtaatccccg aagtcgagcc cttcgtcctc ttcggtctcc acgcctgcga catctacggt 300 ctgaagatac tcgacagcgt atatctggat gagtacccag acaagtacta caaagtcagg 360 cgtgaaaaag gcataatcat cggtataagc tgtatgcccg acgagtactg cttctgcaac 420 ctgctcagga cggacttcga gcacgacggc tttgatctgt tcttccacga gctccctgac 480 ggctggctga taaggatagg gacccccacc ggtcatagga tagtcgacaa gaacatcaag 540 ctcttcactg aagtcgcaca ggaggacatc tgcaacttca gagagttcga gaggaagcgc 600 gcccaggctt ttaggtatca tgaggagtgg gacaacatcc actacctcct cgagctggag 660 atggagcacc ccctctggga gaaagaggcc gagaagtgct tcgcctgcgg caactgcagc 720 acggtgtgtc cgacctgccg ctgctatgag gttcaggaca tcgtcaacct cgacggagac 780 acgggataca gggaaaggcg ctgggactcg tgtaagttca ggagccacgg actggtcgcg 840 ggcggccaca acttcaggcc gacgaagaag gaccgcttca taaaccgcta tctctgtaag 900 atgtccttcc actggacact tggaattaac ttctgtgtgg gctgtggaag atgtactgcc 960 ttctgtccgg cgggcattga tttcgtgaag aacctcagaa ttatagctgg attggaggat 1020 gcatcctgcc cgtcaaagct gagcgaggaa attccaaaga aaggttttgc atatgccaac 1080 aacattagag gtgaagacat atga 1104 <210> 165 <211> 990 <212> DNA <213> Thermococcus onnurineus NA1 <400> 165 atggcacaga ataattcact cgtgctgtat gatgttcatg agaccgtgga tgtgtgctca 60 aacgttggct gtgttaagac caaggccact ccatcaaggc tgctctttgc gggtttcatg 120 gctggtgcat acatagcctt tggattcatt tttgccatag tcgctagtgc aagcttccat 180 ccaaagctgg gcactttccc aaacctatct ctcttcaagc tgcttctggg tgcagttttc 240 ccagtcggtc tcatagccgt ccttcttggc ggtgcggacc tctggacggg caacgcccat 300 atagtaacac tttcgaaaat gacgggcagg gcgagcgtta aggatgtgct ctacaactgg 360 atcggcagct acacaggcaa tttcgtaggc tcggtcttct tggcattctt ggcagtttac 420 ggaacggggc tcatggcagg tggtttgttc aaggacgttc tgataggcat tggcaactac 480 aaagtggcgc tcaccccatg gaaggccctc tggctgggaa taggctgtaa ctggcttgtg 540 aacgtggcga tatggctcta cattcgcgcc aaagacactg ccgggaaggt aatcgtaacc 600 tggttcccga tcttcgcctt cgttgccata ggttttgagc acagcatagc caatatgtgg 660 gccataagcg ccagcatatt tgcctcggac ggtgcgataa gctgggtcca gttcttccac 720 aacataatcc cagtcacgat aggaaatgcc atcggaggct tcctctttgt gggcttctac 780 cactggtacc tcgctgacgg tagaaatgcc attaaagagc tgattgactt tgtcgaggtg 840 ctggcactct tcgtctttat catggtgctt atcccagcgg gaatagccta cgccctcagc 900 ggtctcggaa acattgccac atggcttgtg ccactcatca taagtgtcta tggagttgtg 960 atgacgtatt tagtaaggag agcgctgtga 990 <210> 166 <211> 2043 <212> DNA <213> Thermococcus onnurineus NA1 <400> 166 atggaggagt ttaagattgg cctgtgccca tactgtggga tggggtgcag gttttacata 60 aagactctta acgggcagcc cataggaata gagccgtatc ccggtggtgt taatgaagga 120 aagctctgtc caaagggtgt cgccgccgtt gacttcctca gacacaaaga taggctgaaa 180 aagccgctca agagaactga aaacggcttc gtcgagataa gctgggaaca ggcgataaag 240 gagattgctg aaaagcttct ggagatacgc gagaagtacg ggccggatac gttaggcttc 300 ttctcaagtg cccgttgttc caacgaggag aactacctcc tgcagaaaat agcccgcctt 360 ctgggcacca acaacgtcga ccactgcgcg aggctctgtc acgcctcaac ggtcgtcggt 420 cttgctcaga cggttggcgc tgccgctcag agcggctcct acacggacat acccaaggct 480 aaggtactcc tgatatgggg atacaacccg tcagaaaccc acccggttct catgcgctac 540 atcctccgcg cgagggacaa cggggccaag ataatcgtcg tagatccgag gaagacgagg 600 actgtctggt tcgccgatat gcacctccag cttaagcctg gaacggacat agtcctagcc 660 aacgccatga tgcacgtcat cattgaagaa aggctctatg acagggagtt catcatgaac 720 cggacgaagg gctttgagaa gctcatagca gctgtccaga agtacacgcc agaatacgcc 780 gaggaaataa ccggtgttcc cgccaagctc atcagagaag ccgctataac ctttgctact 840 gccggacggg gcatcgtgat gtgggcaatg ggactgacgc agcacgtcac tggggcggcc 900 aacgttaagg ccctcgctga tctggctctg atctgtggct acgtcggaag agaaggaaca 960 ggtctcttcc cgatgcgcgg tcagaacaat gttcagggag catgtgacat ggcagccttg 1020 ccaaacgtct ttccaggcta tcagaaggta actgacgacg agaagaggaa gcacgtggcg 1080 gaaatttggg gcgttgaaga tctgccctcg aagccgggcc ttactattcc agagatgatt 1140 gatgcggctg ctaaaggcga gttgaaggca ctctacataa tgggcgagaa tccggtcatg 1200 agcgatccga acacgaagca cgttatcgag gctctcaaga acctcgaact tctcgttgtt 1260 caggatatat tcctcaccga aacggccgag ctggctcact acgtgctccc agcagccgca 1320 tacgccgaga aggaaggatc attcaccgcg agcgagaggc gcgtccagtg gaacttcaag 1380 gcgattgagc cgccaggaga agccaaaccg gactgggaga tactgacgat gcttggaaag 1440 gctctcggcc tgccaaagtt cgactactca gacgttgaag atattacgag ggagataacc 1500 ctcgttgctc cgcagtaccg tgggataacc cccgagaggc tcaagcgaga ggttatgggt 1560 gtgcagtggc cgtgcccgag cgaggatcat cctggaacgc cgaggctgca cgtcgagcgc 1620 ttcgccaccc ccgacggaaa ggccaacata atccccgtag agttcaagcc acctgcagaa 1680 gagcccgatg aggagtaccc attcatactg acgacattcc gcatcgtcgg ccagtaccac 1740 acactcacga tgagtaacag gagtgaaagc ttgaagaagc gctggtccag cccgtacgcc 1800 cagataagtc cggaagatgc aaagaagctg ggtatacagg atggtgaaat gataaggata 1860 gttacgagac gtggaagcta cacctgcagg gcggtcgtta ctgaagatgt ctcggaaggg 1920 gtgatcgcag ttccgtggca ctggggggcc aatatactca cgaacgatgt cctcgatcca 1980 gaagcaaaga ttcccgagct gaaggtggcc gcatgtaggg tggagaagat tggggggtgc 2040 tga 2043 <210> 167 <211> 492 <212> DNA <213> Thermococcus onnurineus NA1 <400> 167 atggagaaaa agctgttcat aaacctcggg cgctgcattg cctgccgcgc ctgcgaggtg 60 gcctgtgaga aggagcacgg aatttcattc atcacggtct atgagttcag ggacatagcg 120 gttcccctca actgccgcca ctgtgagaag gctccgtgta tcgaagtctg cccgacgaag 180 gccatctatc gcgacgaaga tggcgcagtt gtgatagacg agtccaagtg tatcggctgc 240 tacatgtgtt cggccgtctg cccctacgcg attccgatag ttgacccgat aaaggagctg 300 gctgtgaagt gtgacctatg tgccgaaaga aggaaggagg gcagagatcc gctctgcgct 360 gcggtctgtc ccaccgatgc gataatctac gctgacctca acgagctgat ggaagagaag 420 aggaggcgca aggccgagcg catcgtcgaa gcccagagga aggcggtcga aacgctcgcc 480 tacttcgggt ga 492 <210> 168 <211> 378 <212> DNA <213> Thermococcus onnurineus NA1 <400> 168 gtgctgaagg tggagctctg tgtggggtgt ggggtttgtg caaaggcctg cccccactcg 60 gccatttcag tttttgaaga tagtgtgagg aggatagtct tcgacccgaa gaaatgcgaa 120 gaatgctcct ttgagtgcaa cgaagcctgc ccaacggggg cgctggaagg gaagtcagac 180 aaaagggagc tggtctttga gtttgcctac tgtgccatct gcgggaaaag gctcaacatc 240 gtgaaggaag aagccgaata tcttgcaaaa aagctgattg agctgggtga aaaccctgag 300 attgcctttc tctgtgatga ctgcaagagg aaaaggctgt ttggcgttgc caacaaatat 360 gaggcttacc tggggtga 378 <210> 169 <211> 1059 <212> DNA <213> Thermococcus onnurineus NA1 <400> 169 atgagcggga tgaggtttgc gttcctgtgt agggaaagac cagaaccaac tgggaagaag 60 atagccgtta tcggagccgg accggcaggc ttggcggcaa ctggctacct tgtctgtcag 120 ggtcatgagg ttcatgttta cgacaagttg ccggagcctg gaggattaat gctatttggc 180 ataccagagt tcaggattcc aatataccgc gttagagaag gctatgaaga attagaaagg 240 gtctacaatg tcaagttctt caccagaacc aaagtgtact tcgggaatct ggaaggagaa 300 tcaggagacg agttcgttga gaacagggta gacttcaagg aactcgtgga gaagtacgat 360 gcggtactaa tagcaacagg aacgtggaag tgctggattc caaacattga gggagcggag 420 cttgagggtg tcttcccggc cctcgagtat ctctttagga taaagagcgc caagctcggc 480 cacatggatt ggggcaaggt cacaccagtg gagggcaaga aagtgctggt cgttggtgcc 540 ggccacacag ccgtcgatgc cgcattggag agcgttctcc tcggagcgga taaggtgtac 600 ctcagttacc gcaggacgat aagggaggct cctgcggggg cctacgagat taacctcctc 660 cagcagaggg gtgtgaagtg gctggagagg acgatgccgg tcaggataat aggtgagaac 720 ggaaaggtca gagctgtgga gctggtgaag accaagctca gtgaacccga cgagagcggc 780 aggaggagac ctgttccaat agaaggttcg aacttccaga tagacgtgga ttatgtcatc 840 ttcgccgtcg gtcagagccc cactccaccc ttcgcagaag agatcgatat agccgtcgat 900 aagaagggca ggatcgtagt tgataacagg cacatgacga gcagggaagg tgttttcgcc 960 gcaggagacg tcgttttagg cccgtcaaag gttggtaagg ctgttaagga tggcctgtat 1020 gctgctgagg ccatgcacat gtggctgatg gggaggtga 1059 <210> 170 <211> 507 <212> DNA <213> Thermococcus onnurineus NA1 <400> 170 atgacgagga gaatccttca cgttgattac agcctttgta ttggctgtga gacgtgtgag 60 gcagtctgtg acttcctcca cggtggcaag cccaacataa ggatttacta cactgtcacc 120 ggacttccga ttccaataaa ctgccgccac tgcgagaggg caccctgtat ggacgtctgt 180 cctgcaggtg caatttaccg cgacagcgac ggagccatca taataaaccc tgacaagtgc 240 ataggctgct acatgtgtct tgccgtctgt ccatttggcg tgccgagctt tgacgtcaag 300 actaaggcag tcacgaagtg cgatatgtgt gccgacagga gaaggcttgg catggaacct 360 gcctgcgccg agatgtgtcc cgcagaggca atattctttg gaaagcccga agaggtcgag 420 gacaggataa gacgcaggac tgccgagagg atagcacgcg agaggatagc tgccgtagac 480 atggaaggtg ttgggaggat gctttaa 507 <210> 171 <211> 1221 <212> DNA <213> Thermococcus onnurineus NA1 <400> 171 atgttgtggg agtcccagat ccccataaat caggtgttcg aacttcgctg cagatccatg 60 acatacttcg gtgttggagc cattaacaag ttctacgaca tagccaagga tcttaaggaa 120 aaccgcggca taaccaaggt cattctcgtc actggaaaga gttcatacaa gaagtgtggc 180 gcctgggacg ttgtcaagcc cgcccttgag gagtacggta ttgagtacgt ccactacgat 240 aaggtcggcc caaacccaac cgttgatatg atcgatgagg ccacccagct cggtaaagag 300 tttggagccc aggctgttat tggcattggt ggtggaagtc caattgacag cgccaagagc 360 gttgcgattc tgctggagta caccgacaag actgccaggg acctttacga gcttaagttc 420 accccaacga aggccaagcc catcatagcc gtgaacacga ctcacggaac tggaactgaa 480 gtcgacaggt tcgcagtggc ttcgattccg gagaaggagt acaagccggc catagcctac 540 gactgtatct acccactcta ctccatcgac gacccggcgc ttatgacaaa gcttccggcc 600 gaccagactc gctacgtaac cattgatgcc cttaaccaca tcacagaggc cgccaccacc 660 aagtttgcta gcccttactc aatacttctc gcccaggaga ccgccaggct gatattcgac 720 tacctcccag aggccctggc tcacccggac aacctgcagg ccaggtacta cctgctctac 780 gcctccgcga tagcgggtat atccttcgac aacggcctgc tccacttcac ccacgctctt 840 gagcacccgc tcagcgccgt aaagccagac cttccacacg gacttggcct cgccatgctc 900 ctgccggcag ttatcaagca catctacccg gccaccgcca ggatactcgc cgaggtctac 960 aggccactcg ttccagaagc taagggagtc ccgggagagg ctgaactcgt cgcaaagaag 1020 gttgaggagt ggctcttcaa catcggaatc actcagaagc tgattgacgt tggcttcact 1080 gaggaggacg ttgataagct cgccgaactc gccatgacca ccccaagcct cgacctgttg 1140 ctttcacttg ccccgattga ggctaccaag gagaccgtcg cggccatcta ccgcgactca 1200 ctctacccgc ttaacaagtg a 1221 <210> 172 <211> 1104 <212> DNA <213> Thermococcus onnurineus NA1 <400> 172 atgggagtta tagttgtaca accatcagga aggtgtgacg caacctgtgc aaattgcatt 60 tggagagaaa ggttaagtgg agtaatgctc cctggggatg ttcttcctag aatagcctct 120 cttttagatg ggttcagatt cgatgaagga atattgatgt gccctaaccc gtttcttcat 180 ccaaaaataa agattatcta cgatgagctg agggacatat caaaaagggt gaccgttttt 240 atacccttaa ctgccagtct ctccaacctc agagtagatg tgcttgctga tgtggacatg 300 atctctataa ttgttccgcc catgatagat attaaaagag gggatactct aattagagcc 360 cttgaatcga gaggtattga tcacattgag gcttatttgg tatttaactc gagctcggat 420 ccaggggaga tccttaggaa aatcggggag tgcatgaagc gaggattaag aataactgtt 480 ggaccctccc ttttcagccc tccatccgga gatatgttca ttgaatcaat aagtgccaga 540 aaagatgtcg aacttggcct gcattatgga aggaaatatc tttattctgc tatgaaagtg 600 ttcctaaatg attatcccat tactctgctt atgtccccca tggatccatg caggcatctt 660 tatgttaatc catatggcat tatatcaaag tgtccaaact caaatttttc agtgagctac 720 cgggagatga ctagagaatt acttcggaag atattctttt ccccatgtcc caacaataag 780 aatccgagtt ttgtacccaa agttgaaata tcctttgtta cgtcatcagg cataaaaatt 840 cccggagata ttatggaact ccttgaactt atttctcaga ccagatcatt tagagcggca 900 tgtaagatta tgggagtttc tccatcaaca tactgggaaa ggatcaggga tattgaggaa 960 aaactcggga gaagattgat tgtctccgtt aagggaggtc gaaaaaaagg aataactgta 1020 ctcactggag ttgccttgga tctcctgaaa gaataccaac ggatccgaga gagagtttta 1080 ctgtctctaa atgaaaggtt ctaa 1104 <210> 173 <211> 639 <212> DNA <213> Thermococcus onnurineus NA1 <400> 173 atgccagctt tttccggttc caacatggag aagcttacaa tttacataaa tccagagaga 60 tgcacggggt gcagggcctg cgaaattgcc tgtgcagttg aacattcaat gagcaaaaac 120 ctctttggcg caatttttga aaaaccaacc cccaaacccc gactccaagt tgttgtcgcc 180 gacttcttta atgttccaat gagatgccag cactgtgagg acgctccctg tatggaggcc 240 tgcccaacag gagcgatctc aaggaccaaa gaaggctttg ttgtccttaa cgccaacaag 300 tgcataggct gtctcatgtg tgtgatggcc tgtccatttg gccatcccaa gttcgagccc 360 gaatacaagg ctgtgataaa atgcgacagt tgtgttgata gggtcagaga aggcaaagag 420 ccagcatgtg tcgaggcctg tccaactaga gccctgaagt tcgggactct cggcgaaata 480 ctggaagagg ttagaaagga gaaggcagag agtctcatat ctgggctgaa atcgcagggg 540 atggtctaca tgaagcccgt ctccgagtca aagaagaaag aggatcttgt tagacctatg 600 gatctgtatc ttgcctattc aaatgtagtg tggtattga 639 <210> 174 <211> 1893 <212> DNA <213> Thermococcus onnurineus NA1 <400> 174 atggccggaa agaaggttcc ctcaaagcaa gtctccataa ctccaggtgt tggaaagctt 60 attgagaaag ccgaggagga tggggtcaag actgcctggc acagattttt ggagcagcag 120 cctcagtgtg gattcggtct cttaggtgtc tgctgtaaga actgtacaat gggaccatgt 180 agaatcgatc cgtttggggt tggcccaact aagggagttt gtggtgcgga tgcagataca 240 atagtagcaa ggaacattgt aagaatgata gcggctggta ctgccggtca cagcgatcac 300 tcaagagatg tagtccatgt attcaagggc attgctgaag gaaagttcaa ggactataaa 360 ctaacagatg ttgaaaagct caaagagctg gctaagattc tgggtgtcga aacagagggc 420 aagagcgaaa atgaaattgc attggaagtc gcccacattc ttgagatgga gttcggaaaa 480 caggatgagg agccagtaag attacttgca gcaacagcac caaagaagag gattaaggtc 540 tgggagaagc taggagtctt accaagagcc atcgacaggg agatatgtct cagtatgcac 600 agaacccaca taggctgtga tgcagaccct gcaagccttc tactgcatgg tgtgaggact 660 gccctggccg acggctggtg cggctcaatg atggccactt atctgagcga cattctcttt 720 ggaacaccaa agccgataaa gtcgctggcg aacctgggag tcttgaagga agacatggtc 780 aacataatcg ttcacggcca caacccgatt ctctccatga aaatagcaga gattgcccag 840 agtgaagaga tgcagaagct tgcagagcag tacggagcaa agggaattaa cgttgctgga 900 atgtgctgta ccggaaacga agttctctca agaatgggag ttcaggtcgc tggaaacttc 960 ctaatgcaag agctggcgat tataactggt gcagttgagg ccgtgatagt tgactaccag 1020 tgcctaatgc cctcattagt tgatgtcgct tcatgttacc acactaagat aataactact 1080 gagccaaagg ctcgcattcc gggagcaata cacgtcgaat ttgaacctga gaaagcggac 1140 gagatcgcca aagagatcat caagattgca attgagaact ataagaacag agttccggca 1200 aaagtctaca ttccagagca caagatggaa ttggttgctg gatttagtgt cgaggcaata 1260 cttgaagccc ttggtggaac actggagccc ctcataaaag ccctccagga cggaacaata 1320 aagggaatcg tcggaatcgt tggatgtaac aatccaaggg tcaagcagaa ctacggtcac 1380 gtcaccttgg ccaaggagct catcaagagg gacatcctgg ttgttggaac tggttgctgg 1440 ggaattgctg cagcaatgca tggattacta acccccgaag cagctgaaat ggccggtcca 1500 gggctgaagg cagtatgcga agcgctcgga attccaccat gcctgcacat gggaagctgt 1560 gttgactgtt cgagaatcct gctggtcttg agtgcccttg ccaatgctct gaatgttgac 1620 atttcagact tgccagttgc tggctctgct ccagaatgga tgagcgagaa ggcagtggca 1680 ataggaacct acttcgttgc aagcggcgtc ttcacgcact tgggagttat cccaccagtc 1740 cttggaagcc agaaggttac caaactcctt acggatgaca tcgaggatct ccttggaggg 1800 aagttctacg ttgagacaga tccagtgaaa gcggcagaaa caatatacaa cgtgataatt 1860 gagaagagga aaaaacttgg atggcccatc taa 1893 <175> 175 <211> 810 <212> DNA <213> Thermococcus onnurineus NA1 <400> 175 atgaaaatac tagtagctgg aaaaggaggg gttggcaaga caacaacaac agcattactc 60 gcccatataa tcgccgacaa aggctacaat gtactgaccc ttgatacaga ctccgttcca 120 aaccttgctc aaagccttgg tgtcccatat gatgaggcat tagatatcgt tccactctcg 180 cggaacgagg agcttgccca agagagaacg ggagcaaaac ctggttccgg ttggggggta 240 ctgttctccc tcacaccgaa agttgacgac ttagtggaca tgtacggaat aaggataaaa 300 ccgaatctaa gtctcgtcgt ggtcgggagt acagaacagc caaaggaagg ctgtctgtgt 360 ccgtcgatag cactcgccaa agcattcctg aatcatgtcc ttctaagaga gaaggacatt 420 gtaatagttg atagtgaggc tggtgcagag gtgttcggta gaggtctagc ggaaaaattc 480 gatgttatgc tttgtgttgc ggaaccaaca cttaaatcgc tcaccatagc caagaaactt 540 ctcaaaatgg gtgaggagct caacatttcg aacttaatgc tcataattaa caaagtacac 600 gattccatgg aagccgctaa gctctattca cgtgtcttct ctgactatct ggttccctat 660 cacctggtgc attttgacac gtccgtaatt aaagctgaca atgaaggtct gggtgtcaat 720 tccataccaa gagattctag gatttaccgt gatgttgaat ctcttgcaaa taaacttctg 780 agcatgaaaa aacgctcaaa ggtggtatga 810 <210> 176 <211> 195 <212> DNA <213> Thermococcus onnurineus NA1 <400> 176 atgtgtgagt caaaagccgt tgttgtggaa aatggaaagg aagaaatcat aatggaggac 60 gtggcgtttt tgtattttaa ggaaggaaag ccagtgataa gagacattct cggtagagag 120 atgctactcg aagactatga gctggacagc attgattttc tgaggcacat catgaggttt 180 aaactcagga ggtga 195 <210> 177 <211> 1905 <212> DNA <213> Thermococcus onnurineus NA1 <400> 177 atggaggtaa tttttctttt tattgtcatt attctgtcag ttgcatcttt cattggagtc 60 ttttcgagga gcgcaatttt aacgaagtta gtaaatgctc tctccgctct tggctcattg 120 acaatagcct atgccgggat tgtaggtctt aaagagagcg ttgaattaaa tatcactctg 180 ttacatctga aatcggattc cattatcaat gcgttctcaa ctctaacact caaagtcgac 240 ccgctgtcag gctttttcat gataatactc ggaattctgg gcttctgtac atcggtttat 300 ggtattgcat acttagacat gtataaagga gacaagagac tatatgcctt caactatccc 360 ctcttcctgc tcttcatgtt ccttgttctc gtctcatgga atctcttgtg gttcgttgtg 420 ttttgggaac tgatgactct cttctcccag ttcctggtag cgtttgaaag gaacgagaag 480 actctcattg cgaccctcaa gtacttctgc atgactaaag ccgcagcaga ctttatgctg 540 atagccatag tattggtact cataacaatc tctggcggag gtgattacga tatcctctcg 600 tcccagctcg taaactattt ccgctctcat cctctggaga tgtatcttgt aagtgctgga 660 ttcatgatcg gacttggtgt caaggccgcc cttgtgccgt tccacgtatg gcttccagac 720 gcatacgtgg aggcaccaag taacgtctcg tcattgctca gtggggccat ggaaaaaatg 780 ccagtgtata tgatgttccg ctttttcctg agtttcaccc cactaacccc taatattggt 840 ctactcatag cactgttcgg aacattaacc ctgttttttg gtacgatgta cgcactaaag 900 caaacagact caaagcgcct actggcctac catagtgtcg gccagatagg ttacgttgtc 960 tttgcccttg gggcagggat atatctcctt tccaaggggt acaccacatt cggagctctt 1020 gcccttatgg catccctatt ccacgctctc aaccatgcat tcttcaaggg actgctcttc 1080 ctaacagcag gctcgatcct ttatagaact ggaagcaggg atttggacca cttgggagga 1140 ctagcgagat ttatgccgat aacggcattt gctgcactga taggttctct ttccatagct 1200 gggatgccac cattcaacgg ctttgttagc aagtggatga tatacgtttc aacacttccg 1260 actccgactc tcgtttccct gtttggggcc ctagcactat tcataagcgc tgtaacaacc 1320 gcatctttcg ttaagtactt cacttccatc tttgtaagac cgcctgccaa ggagataacg 1380 gtcaaagaag tcccagtatc aatgtgggcg tcccagttga ttctcgcagt tctttgtgta 1440 atttttggtg tttatccagc attgccactg gaagcaatct caaaagcggt tgactcagta 1500 ggcgtgacca ctccatcaat cacggtcttt cccggtctca tagtgtccga cggtattgga 1560 aacatagctc ctctggctct cctggtattc tccggagctc tgaccgcagt gttactagcc 1620 attttcccat acaaaatcag tcttccggtg tggacaactg gcacgagacg gtccctggcc 1680 atgaggcttc cagcgagctc atactatgcc tcctttgagg aagaattcga ggatgtttat 1740 agctggggag aatggtgtgt atgtaccacg aaaagactat gggacgccac aaaagccgtc 1800 ttgtccaact ttgaggaagt atccttcgac ttggacaaga tgatgactgg agcttggcta 1860 atgctcctta tactccttac aatactcggg ggtgttctgt tatga 1905 <210> 178 <211> 882 <212> DNA <213> Thermococcus onnurineus NA1 <400> 178 atgaatgcag tttatgctgc cctcaatcta atcttcatag tactctttgc tccgttatta 60 gacggaatcg agaggaaagt caaagcaaga cttcagtcaa gacaagggcc gccgttaatc 120 cagacgtggc ttgatttatt aaagctcttc agaaggccaa acgtcaggcc tagggagtcc 180 gtaagatggc tgttcgaacc agcaccagca atagcgcttg tatctgtatt ggcggcgtcc 240 ctgttcatcc catcactgct tcctggctct ttagacacat ggggggatat aatcgccttc 300 atatacctct caacgctctc agccgtcgcc atagctctcg gagcgttctc aactggaagt 360 ccatatgccc aaataggatc ccacagagaa gtttcaatca taatggcaga ggaattctcc 420 ttggctttta tagtggccgc actcgcagca tccagtggag gtctctcgtt ctcgcgactt 480 ttccccctcc aactaaaagt atctaccata acaggtgctc tggcattcgc agttatggca 540 tacgtcgcgg gagccagaat cccatttgac gtcgctgaag ccgaaccaga gatagtcgag 600 ggtcccttca tagagttcag cgggaaaggc ctgggaatgt taaagctctc aatctacgtg 660 aaacggctac ttctcaccac gatactcctg aacttcttcc taccccaaga tggcacagtg 720 agagtactag tctacgtcat tggactagtc atcatatcag ttgtttacgc gtcaattgaa 780 gcccactatg gaagattcag gactaaagac gccgccagat tcctcaagcg ttttgcaata 840 gttggaatcc taagttggat tttgggagtg gtggggtggt aa 882 <210> 179 <211> 1623 <212> DNA <213> Thermococcus onnurineus NA1 <400> 179 atggtatttg atatcctcaa aggatgtaaa atactggagc acaatgataa gatgacagtc 60 gccgaggtcg gcgccagcaa tatacgggag attgcaaggg cgttattcga gaggggttat 120 tactactcta gtggcatggg agtagacgaa cggcccataa acgggaggtt tgcaatgtac 180 cacatattca actgcgatac agagggaaga tatgtggttc tcaagataac atcccccgaa 240 gggagccctg aggtaccgtc aataacccct gttatcaagg gtgctgaatg gtcagagaga 300 gaagccatgg acatgctcgg catagttttc agtgggcatc caaagcccga aaggcttatt 360 ctaccggacg attggccaga aggagtctat cccttgagaa aagactttcc ttacaacaaa 420 aagcttccac cgtcaaaacc catagaaaaa gaaagggagc acaaaaaaga cgtcatggag 480 atacccctgg gaccatatca tccctccctt cacgaaccag agtattttga gctctatgtt 540 aaaggagaca aagtcgtaga tgcggaatac aggggatttc acatccatag gggaatggag 600 aagcttgctg aatcacgaat gacaataaac caaatcccat tcctcgcgga gaggatatgt 660 ggaatctgtg gttgcaccca ttccgccgca tactgtcagg cagttgagga tgctgctggc 720 atctacgttc cggagagagc acagtatata aggacaataa tgcttgaggt agagagaatt 780 cacagccacc tcctttggtt cggggttgta tgccatcttc tcggctttga cagcggcttc 840 atgcacatct ggagggctag agaatacata atggacatag cagagctcat aaccggcaac 900 agaaagacct acggaataaa tattgtcgga ggggtaagaa gagacatcac ggaggataaa 960 aaggaaaaaa cgctgaaact tctggacatg gttgaaaaag agagcaggga agtacttgat 1020 aacatcgctg agatgaagga gctcagagaa aggatggagg gtgtcggagt tctaccgaag 1080 aaagaggcca gggagatagg tgtggtcggt cccatggcca ggagctctgg gattgatact 1140 gatgtaaggc gagaccatcc ctacgcggct tacaaggact tggacttcaa agtcccggtt 1200 tacaaagaag gggacgtttt tgcaaggttc ctcgtaagat acgaagaaat ttttgagagc 1260 ttcaatatga taagacaggc cctggaaaat atgcctccag gagaactgat aaatgacgaa 1320 tatgagattc ctccattcaa actcggtatc ggagtcactg aagctccacg tggggagaac 1380 atccacgccg tgataacatg gggagagaac atgatttacc gctggcatcc aagagctgca 1440 acctataaca acctcccggc ggttcctata atgctcagag ggaatgacgt tgcagatgct 1500 cccctaataa tagccagcat agacccatgc ttctcctgta cagaccacgt ctcaataatt 1560 gattctgaaa gcgggaagat tctgtggaga gggccgctta aggagggcgt gaggagggtc 1620 tga 1623 <210> 180 <211> 576 <212> DNA <213> Thermococcus onnurineus NA1 <400> 180 atggtaaaaa atagtctatg ggtttttcat ctcaactccg gctcgtgtaa cggctgtgat 60 atagagatcc taaatatctt tgcaccacga aacgatgttg aaagactcgg gataaagctc 120 gttggctctc ccagacatgc agacgccata gcatttaccg gaccaattac aagggagtgt 180 ctgccaaagg ttattgacgc tctgaaagcg gttccggagc caaaagtggt tctggccata 240 ggagcgtgcg cctgtggagg gggcatatgg tatgatactt actccgtaat aggtggtgtt 300 aaagagctct acaggattct aaaagaagaa tacaacatgg agcctcccgc gacggttttt 360 atacctggct gtcccccaaa gccagaggcc ataatctacg gtgtggctgt tgctagtggg 420 atgctagagt caaaacagaa gaagactgtc tatgtcgagc cggaggaatc tgtggcaaat 480 gagaagctaa tgatcgccga gctcataagt gaaacagaaa agacgaggca ctttatgccg 540 ggaattgtca tcaggggggt tgaggatgag ccttga 576 <210> 181 <211> 781 <212> DNA <213> Thermococcus onnurineus NA1 <400> 181 ttgctacatc agatcatgac gaactgcacc agattgtgga actacagatg gcccgctgca 60 gtgagtgcgg aaagtccttc gacacatata ggcatctgat agcttctctc gaaaaaatac 120 acccttggga aagaaagaat ctccttctgt gccctgagtg tagagcgaaa aaaacgtctg 180 agtggagggt gtgggggtga gatccatggt aaaaaatagt ctatgggttt ttcatctcaa 240 ctccggctcg tgtaacggct gtgatataga gatcctaaat atctttgcac cacgaaacga 300 tgttgaaaga ctcgggataa agctcgttgg ctctcccaga catgcagacg ccatagcatt 360 taccggacca attacaaggg agtgtctgcc aaaggttatt gacgctctga aagcggttcc 420 ggagccaaaa gtggttctgg ccataggagc gtgcgcctgt ggagggggca tatggtatga 480 tacttactcc gtaataggtg gtgttaaaga gctctacagg attctaaaag aagaatacaa 540 catggagcct cccgcgacgg tttttatacc tggctgtccc ccaaagccag aggccataat 600 ctacggtgtg gctgttgcta gtgggatgct agagtcaaaa cagaagaaga ctgtctatgt 660 cgagccggag gaatctgtgg caaatgagaa gctaatgatc gccgagctca taagtgaaac 720 agaaaagacg aggcacttta tgccgggaat tgtcatcagg ggggttgagg atgagccttg 780 a 781 <210> 182 <211> 285 <212> DNA <213> Thermococcus onnurineus NA1 <400> 182 atgagccttg aggacacatt cattatcttc atgaagttca ttattccgct ttacctgctt 60 gcatttgtga tttatgcaat tagagctttc agaggtccaa caatacctga cataatacta 120 gcaatagact gcatgagctt cgatatcgca gcattcatgg ccattctggc agtctacttc 180 aaaagcgtct ttctgattag tggtgctata actctagcat tgtgggctta tcttctggat 240 atctacatag ccaaatacct tgtgagtaga gaggtgggag catga 285 <210> 183 <211> 360 <212> DNA <213> Thermococcus onnurineus NA1 <400> 183 atgagtgagg tgctgttcta cctcggagcc atcatgataa tcatcggcgg catctgcgac 60 ctctttgggg ccataggcct actccgcttc cccaacttct acgtcaggct acacgctgca 120 actgttggaa ctatcggggg tgcagtagtg ccgttattcg gtgtcggatt tttagcattg 180 ggtagcaatt tccttcccca caagtatgcc atagcaggag ctagtttcgt cacggggata 240 atagtgcttc tggcagcgcc tgccggtgcc catgccttag cgtacgccgc tcacaaagct 300 aagctcgtcc aatggaaccc caagtgcgac cacttaacag aggtgaagaa gcatgattga 360                                                                          360 <210> 184 <211> 255 <212> DNA <213> Thermococcus onnurineus NA1 <400> 184 atgattgaag tgcacttact aatgctggca attacaacaa tcatcggatt aatattctca 60 tacttggcaa ttattgaaaa agacctgctt agagccattg gcttttcggc agtccaagca 120 attgcctatg caatcgcatt ttacatccta atggcaccag acattgtcct ggcctacgtt 180 gctatagcgg ttggaatata ttcagcactc ctggttttcg ttgtgagtaa aacagggaga 240 tacgaggtgg tgtaa 255 <210> 185 <211> 771 <212> DNA <213> Thermococcus onnurineus NA1 <400> 185 atgaaaaagc taatcccact tgtaatcata cttgtggcaa tattgggatt ggcttattat 60 attgctccaa aactccccca gcaaactgat gtaagacctc taggtgagtt ttacctccag 120 aatagttatt tcggggatta ctcggctaga agtccagagg tcgtcacctc aatcttgtgg 180 gactatcgtg gtgttgatac actctttgag actgcggtat tcttccttgc gattattgga 240 agcctaactc tctttaggct aaataaaaga caggaaaagg cagcaaagca gaagacagag 300 gaattcacag gaggtcttac catagtagtc aaatcagtta cgaaaattat tgtcgtcatg 360 atccttgcgg tttcggcttc gatagccctg cacggtcact taacgcccgg tggtggcttc 420 cagggcggct ctgctctcgc ggtcgctccg ctccttataa tagcagcata ctcaaaatat 480 accctagaag aaaatggctt ggacaagacg agggcattaa ttctgcgttc tattggactc 540 ctgggaatag cgttagtggc gttagttcca ctccttagcg gaggcttcat catgcagaat 600 cagccagtat ttcccgcaga gatcgacagc cagcttattg gcggttcact gatttactac 660 aacttcttcg aattcctagc agttggagcc ggcttcactg cagtattcct cctactagca 720 atcccagaaa gcatatttaa gcgcttttta cggggggatg tggatgagta g 771 <210> 186 <211> 405 <212> DNA <213> Thermococcus onnurineus NA1 <400> 186 atgagtagtg cggttccctt tatattatca atcataatac tatcactcat cgcgacaatc 60 atgatcggcc tgtatggtat cgtggggagg cccaatctag tcaaaaaact catcgcacta 120 acaattttgg gagatactac aaacctgctt gttgttttac ttggatatcg ctcaatttat 180 cctgtaatgc caccaatatt gcccgactta agcaaggaga cactaagtca gttcatcagc 240 acagcagttg accctctacc acaggcccta gtgattactg cggtggttat tggaatggcc 300 gttaacgttc tcctagcctt tgcaataatc cagatttacc gtttatacgg gactgttgac 360 acgagagagc ttcaacagga gctacttggg ggtggtgaac gatga 405 <210> 187 <211> 486 <212> DNA <213> Thermococcus onnurineus NA1 <400> 187 atgaaaggtg tggtccccac tgcaacgctg atattcatca catacctcat atttacgggg 60 tctgcaagtc catttgactt agttactggg ataatcgtag ccattagtgt cggattgctt 120 attgggagat acttagtcca gaatgataca aaagccatga accctaccag atggctatgg 180 actgtgatat atttcatttg gtacatgctc gtggccgaga ctaagtccca cgttgacgtt 240 atggtgagaa cgattacagg aaagtacgag ccagggattg tcaaagtacc aattggagta 300 aaaacagact atgcaaagac actagtcgca aattcaatta ccaacactcc aggaacagta 360 gttgtagatc tagatgataa gtacatgtat gtgaactgga ttaacgtaac tacagaggat 420 cctgaacagg ttaagggaga aatttctgca gactttgaaa agtttgcgaa gaggatattc 480 gagtga 486 <210> 188 <211> 1563 <212> DNA <213> Thermococcus onnurineus NA1 <400> 188 atggacgttg tcggcattac tccagtcatt ccggtaatct tcgcattcgc actaccttta 60 ttctcaatca taatcaaagg aaacagaaag ataatccagg cttacgtcct aatagggact 120 ggcttggctt tagttagcac tttcaaactc ttccagaaaa cctacgcctc ggacaggcca 180 ttagtctaca ctttcggagg atggagtgca ccagttggtg taatctacga agtcgacaag 240 ctaagcgcct taattgcctt ggtgaccgct ggacttatgt tcctgatctc aatttactcg 300 tatagatacc tagaacacga aaatggtctg gagtggtatt atacgctcta tctgggtctg 360 gaggccggac tattgggagt cctgttgacc ggggatgcat tcaatctttt cgtcatgata 420 gaagttacga gtattgcagc ctatgccctg gtgatgttct atcgcgatag aggcgattca 480 gtgtacgctg gcctcaaata cgccctaata ggtgccgtag gaaccacgat gtactttctg 540 gccctcggaa tactctacgg cgccttcggt actgttaatc ttgccgattt gacggcaaag 600 gttcacggaa tggactttcc tctaactggt gctccagttg gggatatcgt ggttgcctcg 660 ggtgttgcct tggccttggc cacctgggcg ttcctcataa aggccgcaat agtcccgaac 720 cacttctggc tcccagaagc acacccagca gcaccaagcc caatctcagc catactctca 780 ggactagtcg tcaacgtcgg tgtctactca ctcatcagat tcctctacac cgtctatggg 840 ggtacactaa ttccagaatt ggcccgcata gtcagcgtat tgagtaacat cgtcgttgtc 900 cttggtgcag tatcatccct tttcggcgct gcaatgatga cagtccagaa ggacgttaag 960 agactcattg cttattcaac aataatgcac atgggttatc tattcatggc ggttggactt 1020 ggcactcaat taggactaca ggccgcattg ttccacctgg taaaccatgc cattgccaaa 1080 gctctgctgt tcctagcggc aggagctttt attcacgcca ttggttcaag gaacgtggat 1140 gatctggctg gcctgggtaa aaagatgcca gttgcgacgt tcagccttgc aatagctaca 1200 ttaagcctcg taggtgttcc accattaaac gtgttcttca gcaagctgct actcttcaat 1260 gctttaatgg agaagggcct tggtccagcg ttaatcctgg tgataagctc tgtaattgcg 1320 ctggtagctt atgtaagggt gttccacatt atttggctgg gcaaaccaag gaaggatata 1380 aagatcaaag agcatgccag catgagctta gtttgtttgg tattggcggt aatctgtatt 1440 atagtcggac tggcaacccc aattattgtg gacggcctca taaacccagc agcgacacag 1500 gcgatggact ataacagcta cataaatgca gtattggatc ttgccttaaa gactattcgt 1560 taa 1563 <210> 189 <211> 1179 <212> DNA <213> Thermococcus onnurineus NA1 <400> 189 ttgagtgaga ttaccctcaa caaagtgtgc cgaatcgccg gtgaggcgaa gctcgtgctg 60 tatgaggaaa acggaacagt tcaagatgca ctcttcatcg ccactgcccc aattagggga 120 tttgagaagc ttgtagtagg caaaaatcca cttttcgcgg tcgaagctgt catgagaatc 180 tgcggtctct gtcatgcgtc ccacggtata gcgatgagcg aggccataga aaatgccatt 240 ggaataatac caccaagaaa cggaatactg atgagagaag ccctcggcct cgtgaacagg 300 attcagagcc acatgctcga gttcctcatg gttgctgggg acctgctaat cgaggagaaa 360 agggaagaag ttctgttcca gctcatggac ttccacgcca agatcagcga ctaccttctc 420 aagatgggag gcgcagcaac acatccccca aacctcaccg tgggaggaat gttctctgtc 480 cccaagtgga gcgtcttcaa caacctcaag gcacgccttc caaagctgac cgggcagtgg 540 gaagagatag cacatttgct gaccgatgag gacatccaga cagaagttgc tgatgaactc 600 agggagaaga aagcggaaaa caactacctg gtaagcagcc tgttctacgg ggacaggttc 660 aacataaacg ccgagagaat tgagacaatg ccctattatg aatacagaaa ggacaacccc 720 cactcaaagg agtccaccac actcatagcc ttctacggtg gggaaaaggt tgaagctggc 780 ccaagggcaa ggatgaaagt ttaccgggag tttacagatt cttccctcta tggccttcac 840 accgcgaggg ttcaggatac aacgctggca ctcattaggc ttgaagaaat ccttgacagc 900 ataaagatgg acgagccgtt cagaacgaag aacatagttt tcgggccagg caagggtgtt 960 ggagtctacg aggcaccaag gggaacactc atccacttga tcgaacttgg agacgagggc 1020 agggtggttt cctccaagat aatcgtcccc acaatgttca acattcccgt gatggaggag 1080 atggcaaaag gtctgagcgt taaagcggcc gaggccgtta tgcgcctata tgacccatgt 1140 attccatgta cgacccacgt tgtgaggttg gggggatga 1179 <210> 190 <211> 678 <212> DNA <213> Thermococcus onnurineus NA1 <400> 190 atggagaagc ttaaggttct tcatgttgat gtagggggtt gtgagggatg caacgtcagt 60 atcattcgcg catatccaaa gctcatggac ttgatagagc tcgacatatc atacctgcgg 120 aaggatgagt gtaagctcga cgagtacgac gtggcgataa taaccggtgg agcatgtatg 180 aacgaaccaa ggattcttga agagctaaag gagataaggg aaaaagctca cactgtggtg 240 gccttcggtt cgtgtgcaac cttcagcggg atattgcgct tctgccgcgg cgggcaggag 300 ccaaggcccg accacaggaa cttccagccc ataaacagcg tgattaaagt tgattactcc 360 atcccgggct gcccgccaac accacagatg ctccagtcct tcttcaagtt ctacatcaac 420 ggtgacgaga gaaggctgag gctcttcaag gtgagtgccg acataaagaa gctgagcggc 480 tttgacctga tagacgatat agtgcttacg ggcctctgca taggttgtgg tgcctgtgag 540 ctgtcgtgcc cgaccaacgc aatcaagctg atagacaaga ggcctaacct cgttcaggag 600 aagtgtatcc gctgcggcac ctgctatata aggtgtccgc gcgcctcaca gattctgtcc 660 atgggtggtg cgagatga 678 <210> 191 <211> 831 <212> DNA <213> Thermococcus onnurineus NA1 <400> 191 atgatgagcg tttcagaaaa tcttttggga aacgtctttg gaatttatct tgcgcgggca 60 accgatgagg aaatactcaa aagaaaggtt gccagcggcg gtgcggttac agccctctta 120 gcctacgccc tggagaaggg cctcatagat ggcgttgtaa cggccaaaag gacagagggg 180 ttggagggtc aggctgtagt tgcgaggaca agggaggagc tccttgaaac tgccggaaac 240 aagtggagca tagtgccctt cgcctccagg atgaaggcca agatagagga ggaagaccta 300 aagaacgttg ccgtggtctg cctcccctgc caggcccagt tcttcggcca gatgagggac 360 ttcccactcc tggaaagcga tttcggagag aggataaagt acatcgttag tctcttctgc 420 ataggaacat tcgcattcga ggcattcctc aactacctca ggatgaagca cggcataatg 480 gcccaggata tcaaggacat agtccttaag ggggacttcc tcgagatata ccacggcgat 540 tcagtgctct cactgccgat aaaagaggtt tactcatacc tccaagccgg ctgtctggtc 600 tgtactgact acaccggaac ctggagcgac atctcggccg gcttcgtgga gagcgagagg 660 ggatggactg tcctcataac gcgcaacctt aaggcagaag agctcgttaa gagcgccgag 720 aaggacggat acatagagct gcgcgacggc tcccacgtga tgggagaagt cctcaaagcg 780 gccagggaaa agcttgcgag agcgcagaag aacatgatgt atctgctctg a 831 <210> 192 <211> 759 <212> DNA <213> Thermococcus onnurineus NA1 <400> 192 ttgataaaga aagtgaaaat tctcaagtgg caagatgggc tcgttcccac cgaggactat 60 atctgcgtcg aggagacctt tgaaatcttc gcagtacacg aaaaggacga agagtttctc 120 gccgaacttc ctgcttcacc caaccagctg aaggaacttg gagccggatt cgtcgtgtgc 180 ggaggctatg aaagaccgga ggacatagtt gacgtatggg ttgagggcaa ggagatttac 240 gtgaagttga aggatacccc cgccacgggc gagctggttg tgaaacacac cccctgcggc 300 gacccctaca gaatgaagga gggcagaatt ctcagcagga agggcgagga agtcaaaata 360 acccccggcc tcgtattgaa gatatcctcc acgatgacaa cgctggctga gacgtggaga 420 aagacagggg gcacccactg ggcggccctc ttcgatttga acgccaatgt cgttgccttc 480 agcgaggaca taggcaggca caacgccgtc gataaggtcg taggatacgc cgtcctcaac 540 ggactcgacc ttgaaaggct tatcctggca tcgagcggca ggatgccata cggcatggta 600 agaaaggcag tcaacgcggg cattccagta gtggtgacga aatcaccgcc gacggacaag 660 ggcgtggagc tcgccaggga gcacggggta accctaatag gcttcgcgag gggaaggcgc 720 ttcaacgtgt actccgggga gcatcgatta ttgttctaa 759 <210> 193 <211> 2136 <212> DNA <213> Thermococcus onnurineus NA1 <400> 193 atgagtgaaa ggctcgtccc cgtggtctgc ccctactgtg gtgtagggtg caggctatac 60 atcaggagtg ttgatggcta tcccgtaggc atagaatacg ccaaggacat ccccaacatc 120 tcaaacgaac tcggaaagct ctgccctaaa ggcaacgccg tcgttgagta cctcctcgca 180 aaggacaggc tcaagagacc cctcaaggcc aaggaacagg gcaagttcgt tgagataagc 240 tggagcgagg caataaagga ggttgccgag aggctcaagg cttatgccaa ggacgacccg 300 aaccagctca tgttcttcgg ctctgcgaga acattcaacg agcccaacta cctcgtccag 360 aagctggcca ggatgctcgg caccaacaac gttgatcact gtgcaaggct ctgtcatgca 420 ccgaccgtca cgggtctcaa ggctgttttc ggtgccggcg caatgaccaa cacctacaag 480 gacattgaag aggcaaacgt catcttcatt atcggccaca actacgctga gacccacccg 540 gttggcttcc gctacgtcct taaggccaag gagaggggcg ctaaggtcat agtcgctgac 600 ccgaggttca ccaggacggc ctggttcgcc gacatattcc tacagcacta cccgggaagc 660 gacattgcgc tgataaacgg tctcatccac gtcatcatca aggagcggct ctacgacgag 720 aagttcgtga gggagagatg cgttggcttc gatgaagttg tggcagccgt cgagaagttc 780 acacccgagt tcgtcgagaa ggtaaccggt gtcccggcgg aactcatcat tgaagctgca 840 agaaccttcg cgaccgcagg aaagggtgtc ataacctggg ccatgggtct aacccagcac 900 acccacggaa ctgaaaacgt caagctcctt ggaacgctcg cggccatttg cggttatcag 960 ggcaaagaag gtgccggctg ttccccaatg cgcggtcaga acaacgttca gggagcatgt 1020 gacatggcag ccctgccgaa cgtcttccca ggctatcagg ccgtcactga tcctgaaaag 1080 aggaagttct ttgaggagtt ctggggtgtt gagctgagcg gcgaagttgg actgacaact 1140 gtggaggctg cctacgcggc cgacaagggc aaggtaaagg cctactatgt catgggtgag 1200 aacccagtca taagcgaggc caacgccaac cacgtgatgc acaccctcga gaagctcgag 1260 ttcatggtcg tccaggatat cgtcccgacc ccaactatgg agtatgcaga tatagttctg 1320 ccggccgcgg ccatgctcga gaacgagggt tctctgacca atacagagag gcgcgtgcag 1380 tggagcttcc aggcggtaaa accacccgga gaagcaaggc ccgactggtg gattcttagc 1440 gaggtcggta aggccatcgg ttttgacaag accggatccg gtggattcgt ctacaatgat 1500 gcagccgacg ttctcaggga aatcaacgcc tgtactccgc agtatcgcgg tataactcca 1560 gagaggctca aggagaacct tgcaggactc cactggccgt gcccaagcga ggaccatcca 1620 ggaacgaggg tcctctacaa ggagaagttc ctcactccca gcggaaaggc caacctcgcg 1680 gccgttccgg agtacaaggg accagtcgaa atgccggacg aagagtatcc gttcctcctt 1740 acgacccaca gatacgtcgg aatgtaccac accgcaacca tgaccatgag gagctgcgca 1800 ctcaagaagc gctggccaga acccctcgcc gagatacacc cggatgacgc agtgaagctc 1860 ggaataaaga gtggagactg ggttaaggtc gtcacaagga gaggagcata tccgattaag 1920 gcaaaggtca cccgggctgt caagaagggc gtaatagctg tcccgtggca ctggggagca 1980 aacgtcctca ccaacgatgc cctcgacccg gtagcaaaga taccggaaac caaagcctgt 2040 gcctgtaatg tcgccaagat cacagaagaa gaggccagga agctcatgga gaaactccca 2100 ccactcatac ccaagattga ggtcgttagg gggtga 2136 <210> 194 <211> 501 <212> DNA <213> Thermococcus onnurineus NA1 <400> 194 atggctagaa agaccgtctt tattgacttt tcaaagtgca tcgagtgccg cgcctgtgag 60 gtagcttgcg agcgcgaaca cagtggaatg tcattcatca gcgtctttga gtggcaggaa 120 atggccgcta tggccctcaa ctgccgccac tgtgagaagg ctccctgtgt tgaggtctgt 180 ccaaccaacg ccctctaccg cgacaaggat ggagcagtcc tgctcgctcc acagaagtgt 240 atcggctgtc tcatgtgcgg catagtctgt ccctttggaa tacccgagct cgatctcatc 300 aacaagataa tgggcaaatg tgacctctgc gcccacagga gagccgaagg aaagcttcca 360 gcctgtgttg agacctgtcc aacagatgct ctcatctacg gcgacttcaa cgagatagtc 420 aagaagagaa gggagaagtt tacggagaaa accatagaac tcgccaaaac tgcagagcgc 480 atcccgctga cgggggtgtg a 501 <210> 195 <211> 1443 <212> DNA <213> Thermococcus onnurineus NA1 <400> 195 atggaagagc tttttattct ttccttttca attccgctgg ttggaggcct tttactgttc 60 aaactcgacg gtaaaagagc ggattacttc atgctcatca ctgtcatcct tgccacaata 120 ctcaatctcg cgggagttta tgagttctat tcctctggga tgcctactat acacaaaact 180 ctcgtgagct ccacaaccct cggtgaggtc tacggtctct tgatagaccc aatgagtgtg 240 tgcgttggcc tggttgtgat aacagccgga ctgcttttta tgatctatgc aaaagactat 300 atgagcccgg agaacaaaga gcacccagtc tatgaggata agggtaggtt ttacgcttgg 360 atggtgctct ttatcggagc aacactcgca ttcatttact cctcgtcggt tctccagctg 420 ctgatattct tcgaaattat gagcctcgcc tgttggggtg tagcaggcta ttatggaagt 480 aaaaaggcca aaagggcagc atataaagcg ttgcttgtta ccaactttgg agcggtgata 540 ggtctataca ccgcggtagg tataggaatc acacaccttc acgacctgag catatttgca 600 tattctggcc tcaatgacag ccttaaactc gtcgttttta tcggggtcat gatagctgct 660 tttaccaaga gtgcccagtt tccgctttat tcatggctcc cagatgcaat ggtcgcccca 720 acacctgctt cagccttcct ccacggtgca gcgatggttg agatgggtgt ctacctgctg 780 gccagattca tccagttcat gaatcccata cccaaagaag gattttacgt catggcagcc 840 ctcatcatcg ccactcagat aatctgcatc ctgatgtatc ctctccagaa gagcgcgaag 900 aggctgctcg cctactcaac aatagcagag tctggactga tgtacgtggc ccttgcgacg 960 gcagtcctcg ggttgcaggg gggacttcag gcttcaatgt tccagctatt caaccatgcc 1020 tacatcaaag gtctcgcttt cctgacggct ggaacgttca gctacgccct gggaaccctt 1080 gagatggaca ggataaaggg cctcattaaa tcccctgtgg tcggctacag ctggaccttt 1140 gccctgcttg gtttagctgg cgttccacca ttcggcgtgt tcttcggaaa gctgggaata 1200 ctcagcaatg ccaaggcaat ggaagagagc gtcctcatca ttgccatgtt tgttttactt 1260 ctcctcgact cagcagtgtt cctcatggtg tctctgaaga ggatacacgg catggtcttc 1320 agcgagggcg gagaagaagt cgagattaca ccactgatga aggctgtgat ggttatcctg 1380 ctcgtcctgg ccatgctggc cccgtacata gcgtatccac tgatcgtcaa agtggggtgg 1440 tga 1443 <210> 196 <211> 1215 <212> DNA <213> Thermococcus onnurineus NA1 <400> 196 atgttcgacg taacgctcac tctttcactc gatagaactg cagtgttctt cgtactcaac 60 gtcgcgatac tcggcatagc ggcgctagtt gcatcgttca gatacatgag gatatacgag 120 ttcaaaccaa agatacccta ctacccaacg ctcgccatct tcatagtctc gatgcttctc 180 atcccaatgg tccaggactg gctcagtttc ctcttcctct gggagataat gactctcgcc 240 tcatacttcc tgataatcta cgactggccg gaggagagcg tcaagaaggc cggctggaag 300 tacttcgtga ccatgcacct cttcgacaca tctcccctca tgctggcagt gactatgtac 360 tacgccttcc atggaacgtt caactttggg gccataacgg agtacagcaa tgccatagtc 420 gctctctttc tcctgggatt tgcggccaag gccggcctct tcccgctcca cttctggtta 480 ccggacgccc acccggccgc accgagcccg gtctcagcct tgatgagtgg tgccatggtc 540 gaactcggcc tctacggaac catcagggtt ctcaatgctg tgggatggag cgtcgcaacc 600 tggatagtct atctcatcgg cgctatggca gtgctcagca tgctggctgc catattcagc 660 tacgccctcc aggacgacgt caagaggctc ttcgcatggt ccaccatcga caacatgggc 720 tggatgtatc tgctcatcct ggcaggcctc cttggcgttt caggagtgga gaagggcgtt 780 gactactacg tcgtggcaca tggactcgcg aaggcggcgg cgttcatatc aacgggtgcc 840 ctcctctacg ttttcggtac gaggagcctg aagaaggcaa aaggtatgat gaacactgac 900 agcctcaccg cgggactcat gatggcctca atattcgccc tcgaaggtgt tccgcccttc 960 aacctcttca tgaacaagct caatgtgata aagactctcc tgacggtcag cccggccctg 1020 gcatacttca cggcccttga gtgggtgata gcgttcatac tgttcctcag agtggttcac 1080 gcctacatcc tcagcgaagg tgaaccagag gccaagagaa agcttgctgg aagcatagcc 1140 ctctccgtga tagtcctgct catcctctcc atggtaagcc agttcgtctg cgactacata 1200 tgggtgaggt ggtga 1215 <210> 197 <211> 2073 <212> DNA <213> Thermococcus onnurineus NA1 <400> 197 atggagggac tctttacgct cgccgtcatc ctgtatttcc tctccatacc cgcagcgtta 60 gccctcaaaa ggagcttcaa ggcttcaatc agcattggcc acatactcac ggctctagcc 120 tccatagctc tgttagcatt tacctttgtg tcaataccag atatcctcag cgggaaggcc 180 atagaattca catatgactt aggagtggcc cagattccgt tccagattga tgggctctcg 240 ctgataatgt gcttcatctt cggcgccctc ggacttgcag cgtcaatata ttccccgaga 300 tacatggcaa tctacgagaa gtcaggcaga ggctggatgt acataaccat atattcagtg 360 tttatgctct ccatgatact catagtaaca atagccaaca tgttctggtt cattttcctc 420 tgggaggtca tgacgtttac atcgtacctc ctgacgatct gggaaagcga caaagaggat 480 gtcagaaaag ccggctggaa gtacttcgtg accatgcaca tagtgagcac actgccactg 540 ataatcgccc tcgccctgct gtatgcagac gttagctcaa tcgagggact taactttgag 600 agtctagcgg ccttaaaact aagcccagta ttctacgccc tcttcctgat aggctttgga 660 agcaagtcag gcgttgtccc gctgcacttc tgggccccgg aggcctatac ggtcgccccg 720 agcaacgtct ccgctctaat ggctggagca ctggagaagg tcgcggtcta tgccctgata 780 aggactacat gctttatcat gaagccaaac gagactttcg gatatgcagt tgccctgctt 840 ggaacagtaa ccctgacagt tggaaccctc tacgcgttga agcagaccga tgccaagaga 900 cttttggcct accacagtat cggccagata ggctacatct ggctcggcat gggcgttggg 960 atagttttca tagccagggg agatatgtac tcagccttcg gagccatagc cctagcatca 1020 agtctgtacc acctcgttaa ccacacgttc ttcaagggac tgctcttcct gtcgacgggc 1080 tcaatattct acagaacccg cagcagggat ctcaaccagc tgagaggtct ggctaaactg 1140 atgcccttta cggcgctctt cacattcata gccgcaatgt caatagctgg aactcctccg 1200 ttcaacggct ttatgagcaa gtggatgatt tatcagtcaa cgttcctctc gggcaacggc 1260 ctgatagtgt tctttggagt gatggccctc ttcataagcg cagcaacgct ggcttcattc 1320 atcaagttct acacaaccgc atttggagga gaacctactg agtttacgaa ggatgctgag 1380 gaagttccat cccctatgct catcgccaag ggcttcctgg cttcactctg catcctcctt 1440 ggactggttc caagcctcat cctgccgata ctgctttcgc caggggcagc cctagccggt 1500 atagatgtct caggactgat ggacacaaac tactggcttg tcacgattaa agctccgctt 1560 atgccgacag gggcagagag ctacttcaaa ccgctactct ttgcgacact cttcggcgtg 1620 atcttcctcg gcatgtacct gctcttccca atctcaaaga aaacctacag accctggacc 1680 ctcggtgagc ccgtggcgat ggagcactac aagttcaagg cgataaacta ctacgaaccc 1740 ttcgaggagt acatccaccc gctctaccac accggccacg ttctcagcga gttcggatct 1800 gccctgattg gcgcagtcgc caatgcgtac gtctcaacaa caagggctct ccacagagta 1860 tgcgattcta taagcaagag tgtggccggg atcggaaaag agtacgagaa gaagtgcccc 1920 gaagtctacc ttgacgaata cttccttgcc ccactggtca agatagtgag ggtctcagga 1980 gtgcttctag atgagggatt catgaggcca aatgcagcgt tcacaatagc cctggtaact 2040 ctggcggtta tacttgccct gatggtgctg tga 2073 <210> 198 <211> 924 <212> DNA <213> Thermococcus onnurineus NA1 <400> 198 atgacgctcg aaaaaattgc attcgcggcc ctttcactga tgataatcat cctccttccg 60 cccctcctcg acggaataag cagaaagatc aaggctaccg tccaagagag gcaggggccc 120 cccgtcttcc agacctacta tgacctctca agcctgctct caatggagcc gatccttcca 180 acggacagac tgggcttcct catagctccc tatgtggcct ttgcttcagc agtctcagcc 240 gccctgctcc tccccttcgg gaacttcgtc ccagtggcct tcacagggga catcttcgtc 300 ttcctctacg tgctggcgat attctcgata tcgatgatga tggcaggctt cctcgtgaac 360 aacacctact caaacgcggg tgccaacagg gagatgatgc tcatcctcag cgtggagccg 420 atactgggaa tagcgatagg catactcgcg cttaagaccc actcgctcag cgtgagcgga 480 attccactca acctcagcct cacaccctcc gttgtcctcg ctttcatctt cctcgcctac 540 gccgtctata ctgagtgcgc cttcataccc ttcgacatag ccgaggccga aacggagata 600 cttgagggtc cactcgtcga gtacagcggg aagctgcttg gaatcttcaa gtgggccatg 660 ctgataaagc gcgtagccct gatatggctg ttcgcgagct tcatagtcat tccagtcatg 720 aaggggttcg tcgacatcac gacgccctac ggtggtgcag taacgctcgc ggcacagctg 780 gtactcctgg tggtcttcta cgtcatgtcg gccatcatag agtcaacgac ggcccgtatg 840 aaggtaatcc aggccatcag gcagaacacg gtgatattcc ttgcgggaat agtcgcgctg 900 gtgatagctt ccctgggatg gtga 924 <210> 199 <211> 1755 <212> DNA <213> Thermococcus onnurineus NA1 <400> 199 atgtctgaag ttatcaagtt taacgaggct ctgaaaaaga agcgcgtaca caggggagat 60 gaaaaagcca aagtaacgcg ggagtacttg gatgagatta tcgagaagtt cggggagaag 120 ataagggacg tcaagcaggc cgcttacaac cagtggatta taaccgtcga gagggaagac 180 cttccggaga tagtcctcta cttcctcaac cacccggagt ggaaggagac ccagctctca 240 tcgatggtgg ccaccgacga gaggccccta aacggcaagt tcagcatcac ctactggctc 300 agcgttaacg gaaaggcggg tgacttctat ctcggcgtca gggcttacct gccggaggac 360 gacccgaggt tcacctcgat agcggccaag cacaggggcg cgaactggta cgagagggaa 420 gccatggaga tgctcggcct cactgccgaa ggccaccccg acccgaggcg gctcgtcctt 480 ccggacgact ggccgtcctg cgtctacccg ctcaggaagg acttccacta ctcgaacagc 540 ccgccggggg agaagttcta cccctacaag gaaccgaaga aggacgagat agtcgtcccc 600 tacggaccgt atcatgtggc ccttgaagag gcagcacact tcaggctcta tgttaaggga 660 gaaaccataa cagacgttga ctatcgcggc ttctacgccc acaggggcat agagaagata 720 tccgagggaa ggctaaccta cgaccaggtc tgcttcatag cggagagaat atgtggaatc 780 tgcggctgca cacactccac agcctactgc caggcggttg agaacgccgg aggtatagag 840 gttcccgaga gagccgagta catcaggacg atagtcctcg agatagagag actccacagc 900 cacctgctca actttggaat agtctcccac ctcgttggct acgactacgg cttcatgaaa 960 gcctggagga taagggagca cgtgatgtgg ctcgcggaaa ggctaacggg caacagaaag 1020 acctacggaa tgctccttgt cggcggcgtt aggagagacc ttctggagta cagaaaatcc 1080 ctgatagaag acgtcctcaa gaagataaag accgagttca gtgagctcgt cgatgaggca 1140 atctcaacga gcaccttcgt gaagcgcctt gaaggcgttg gggttctgcc ctacaaggtc 1200 gccaaggagt gggacgttga tggacccctt ggcaggggct ccggaaggga cttcgacgtg 1260 agaagggacc acccgtacgc ggcctacaag tacctcgact tcaaggtccc agtctacaag 1320 gagggtgacg ttctggcaag ggccctcgtc agaatagagg aagttttcga gagcatctgg 1380 ataatagagc aggccctcga ccagatgccc ggaggagaca ttctggcgga gtacaaggag 1440 atacccccgt actcggaagc gataggcatg actgaggcac cgaggggcga gaacattcac 1500 tacgtcatga ccggcgagaa caacaaggtc tacaggtaca gggccagggc ggcaacctac 1560 aacaacctgc cggctgttcc cgacatgatg cgcggctaca ccatagccga cgccccgctc 1620 atagtggcga gcatagaccc ctgctactcc tgtacggaga gagttcaggt agtcgacgtc 1680 gaaagcggga aggttagggt tctcagcgag acggagttca acaagctctc cataaaggcc 1740 tcaaggaggg tctga 1755 <210> 200 <211> 498 <212> DNA <213> Thermococcus onnurineus NA1 <400> 200 atggccgtga cgctgaagta ccccttcgtg aagcttgaag cccctccgga gtacagagga 60 attccacaga tagacgcgac cctctgcata ggctgcggtg cctgcgttaa cgcctgtccg 120 ccagatgcac tcctcaggat agacgactac aacagaggag ttagagaaat tgtcctcgat 180 gtgggaaggt gcatccgctg tgctcgctgt gaggaggtct gtcccaccgg agcgatcaag 240 ctcacgaacc tcttcgaggc cgcttcgccc gacaggatgg accacgtgga ggttgttagg 300 ctcaggctcg tgaaatgcaa aaactgcggc aggtacgccg acttcactga gaggcaggtg 360 agaaaggccc tccagattct ccccgaggag atcatcgaaa gggaagctct ggaagagaag 420 gtctggatct gcagggactg caggaggaaa gggacagttg atggaaccat agaagccagc 480 aaggaggtgg ttctatga 498 <210> 201 <211> 822 <212> DNA <213> Thermococcus onnurineus NA1 <400> 201 atgagcggaa agccgaagct ccgctccata tgggtcttcc acctcaacac cggctcgtgc 60 aacggctgtg acatcgagat aatcgacgtg ctcacaccgt tctacgacgt cgagcgcttt 120 ggaatcaagc tagttggctc gccgagacac gctcatgcac tcctcgtctc gggtccgctc 180 acgagacagg cctactacgg cgccaaagag accataaagg cgatgcctcc ggagccaagg 240 gtaatagtcg ccatcggaac gtgcacctgt agcggaggga tattctacaa cggctatcca 300 gtctacagaa ggcccgagag cggtagggag ggaagcgagt atccacggag gggaggtata 360 gcggagctca tcgctgactt gagggacgag ggcgagaagg tcggtccggt catctacatc 420 cccggctgtc caccgagacc ggaggagata atctacggca tagcacagct cgtgggactc 480 gtcgagaaga agctcagcta tcaggagtac agcgacgagc tggttccctt caagctccca 540 gaggggccgc tggaggagcg catcaggctg acccttatgg agaggctcag gcacctcgtg 600 ggatacctcg acagggaaaa gatcctcgag gatttcatgg ggctcgttaa agaggccgag 660 aagagcgaga atcccaggga ggagctggcc aggctcgtca aggactacgc cgccaaatgc 720 ggggacgtta gactgggctt ctgtatgatg cttctcgaaa gagagtactg gagggtcaaa 780 gatgccctgg atgctggtaa agagttcgta tattgggttt aa 822 <210> 202 <211> 282 <212> DNA <213> Thermococcus onnurineus NA1 <400> 202 atgccctgga tgctggtaaa gagttcgtat attgggttta agacctacct cgcgggtgct 60 ctctcccaca cggagggaga cttcgaggtg gaagaaatag tcggggagat aagccccaga 120 gcagcccacc tgcttagaaa atccttcgaa cggtcatact tcacgctcgc cgatgctccc 180 ctgattccct tcgaagaact ggatgagggg gataggcggc taatcttgaa ggccctcagg 240 ggccttaggg agaatgaaag gctcaaaata gagaggaggt ga 282 <210> 203 <211> 1029 <212> DNA <213> Thermococcus onnurineus NA1 <400> 203 atggctgaaa caaaggaaaa aatcctgtat ggggtagata caacctttga ggccgtagca 60 aagaaggcca caccaaagtt caagacaacc ccaggtaggc tgctcttcgc cggcttcatg 120 gctggtgcct ttatcgcctt tggtttcctc ctggcggtgg ttgcagcggc tggctacagc 180 ccgaagctct tcccagacac aggcaacata tccaccttta agatactcct cggtgcggtc 240 ttcccggtcg gcctcatagc ggttatctta gcgggagccg acctctggac aggaaacgtc 300 cagttcctca gctcggctaa ggcaaagggc tacgccgact ttaagtgcgt cctctacaac 360 tggttcggaa gctacggtgg aaacttcata ggctcgatat tcctcgcgct cctggccgtt 420 ccgctgaccg gactcttcgg ccacgttgga gacccgaaca ccttcggcca ggtcacggta 480 ggcatagcca ccggcaaggt ctcgaaggac atactggcat tgttcttcct cggtatcggc 540 tgtaactggc tcgtcaacgt cgcaatatgg cagtcggcca gggttcagga cggtgccggc 600 aagatactcg ccatatggtt cccgatcttc gccttcgttg ccataggctt cgagcacgcc 660 atagccaaca tgtgggccat tccagcgggc atactcctca gtgactacgc cataacctgg 720 acccagttct tccacaacgt aatcccagta accttcggta acgccattgg cggtttcctc 780 ttcgtgacct tctactactg gtacctcagc caccccgagc tgaccactga ccgcctcatc 840 aaagagatca ttgacttcct tatcgttttc atagccttct gggccgtcgc ggcgctcatc 900 ccagcgggaa tcggcatagc cctcgatcag gcccttggca agggtgccat gtacctcgtc 960 ccgctggtgc tctcggccta ctacatcgtc ggtgccttcg tcctctacaa gaaggccagg 1020 ccggcctga 1029 <210> 204 <211> 282 <212> DNA <213> Thermococcus onnurineus NA1 <400> 204 atgctcgaaa gcacgttcat gacgctcatg aagctcatca ttcccctcta catagccgcg 60 ttcgtcatct atgccgttag ggcctttaaa gggccgagta ttccggatgt tatcctcgca 120 gtagactgta tgagctttga tatcgcagcc tttatggcca tcctggcagt ctacttcaag 180 tccaccttcc tcgtgagtgc ggccataata ctcgccctct gggcgtacct gctggacatc 240 tacgtcgcaa agtacctggc gaacggggag gtgggagcat ga 282 <210> 205 <211> 360 <212> DNA <213> Thermococcus onnurineus NA1 <400> 205 atgagcgaga tactcttcta ccttggagca gtcatgataa tcatcggcgg catctgcgac 60 ctctttggag ccataggctt actccgcttc cccaacttct acgtcaggct acacgctgca 120 acagttggaa cgattggtgg ggcggtggtt cccctcttcg gtgttgccct gcttgccctc 180 ggtgcggact tcctacccca taggtacgcc atcgctgggg ccagcttcat cacggggata 240 atcgtcctcc tcgcagcgcc agcgggagct actgcactag cctacgccgc ccataaggcc 300 cggctcgtgg agtggaagcc aaaggtcgac cacctggcag aggtgagggg cgatgattga 360                                                                          360 <206> 206 <211> 255 <212> DNA <213> Thermococcus onnurineus NA1 <400> 206 atgattgaaa tccacctgct aatcttagct accgtcacag tcctcggcat ggtcttctcc 60 tacctggcca tcacagagaa ggacctgctc aaggcggtcg gattttccgc cgtacaagcc 120 atagcctacg ccatagcctt ctacatcttg atggcgcctg acattgtcct ggcatacgtt 180 gccatagcag taggaatcta ctctgccctg ctcgtcttcg tgataagcaa gaccgaaagg 240 tacgaggtgg tgtga 255 <210> 207 <211> 756 <212> DNA <213> Thermococcus onnurineus NA1 <400> 207 atgaagcgct gggtcacgct catcctcctg ctcaccgcgg tcatcacgct ggcctacgtc 60 ttccaagtcc cccagccaga ggacgtcaaa cccctcggtg agttctacct tgaaaacagc 120 tatttcgggg attactcggc caagagcccg gaggttatca cctcaatcct ctgggactac 180 cgtggcattg acacgctctt cgagactgca gtgttcttcc tcgcaataat aggcagcctg 240 accgtcttca ggctcaccaa ggagcaggag aaggaggtta aaacggagcc aacgcaggtg 300 gaaccactcc ccctgccgat taggacggtt acaaaggtta tcgttgcgat gatccttgcg 360 gtttcggctt cgatagccct gcacggtcac ttaacgcccg gtggtggctt ccagggtggt 420 tcggctttag cagttgcccc attgctgata atcgcagctt actctaagta tgccctcgaa 480 aagaatggct tggacaagac cagggcgtta atcctgcgtt ctattggact cctgggaata 540 gcgttagtgg cgttagttcc actcctcagc gggagctaca taatgcagaa ccaaccagta 600 tttcccgcag agatcaacgg ccagctcatt ggcggctcac tgatttacta caacttcttc 660 gaattcctag cagttggagc cggctttaca gcagtattcc tcctcctggc gattccagag 720 gagaaattca agaaaatcct gggggtgagg agatga 756 <210> 208 <211> 405 <212> DNA <213> Thermococcus onnurineus NA1 <400> 208 atgataggct tcctctggag cctcgtggta atttcactca tgacgacact gctcatcggc 60 ctctacggaa ttgccagaag gcccaacctc gtcaagaagc tcatagcact gactatcatc 120 ggtgacaccg cgaacctgct cgtggtcatg ctcggctacc gcctgaccta tccgacggct 180 cccccgatcc tcccgagcct tgagaagagc gcactaggga gcttcctgag cgccgcggtt 240 gaccctctcc cccaggcgct ggtgatcacc gccgtcgtca ttggaatggc tgtcaacgtg 300 ctgatagcct tcgcaatcgt tcagatttac aggctctatg gcacgctcga cgcgagggaa 360 ataggtgtaa aacgctcatc gccgctggag ggtgagaaga tatga 405 <210> 209 <211> 486 <212> DNA <213> Thermococcus onnurineus NA1 <400> 209 atgaggggtg tgattcccac cgcactgctc gcgttcatta cctacatcct gttcactggc 60 tccgcaacgc cctacgattt ggcaactgga gccatcgtcg caatcgccgt aggactgcta 120 gcgggcaagt tcctcgtgaa gaacgacgct aaagcgctga atccggccag gtggctctgg 180 cttggcgctt acttcctctg gtacatgctg gtcgccgaag tcaaaagcca cattgacgtg 240 atgctcagaa tcattacagg aaacgtcaag cccggaatag tcaaagtgcc tatcgacgtg 300 aagaccgagt acgccaagac cctcgtggcg aactccataa ccaacacgcc cggaacggtc 360 gtggtggaca tggacgagaa ctacatctac gtgaactgga ttgacactag cacggaagac 420 ccagaagaag ctagaagtga gatctccgcc gactttgaga aatacgcgaa gaaaatattc 480 gagtga 486 <210> 210 <211> 1569 <212> DNA <213> Thermococcus onnurineus NA1 <400> 210 atggacatcg tgggactcac ccccgtgata ccaatactct tcgcctttgc tctccctctc 60 acgtcaataa tagtcagggg caacaggaag ataatccaag catatgctct aataggcacg 120 ggcttgactc tactcgcctc ctacgagctt ttcagacagg tttataactc cagcaagccc 180 ttaatctata ccttcggcgg ttggactgcc ccggttggaa taatctacga agtcgacagg 240 atgagcgctc tcttcgccct agttactgcc gcgctgatgt tcctgattgc gatttacagc 300 taccgctacc tggagcacga ggagggcttg gagtggtact ataccctcta cctcggcctt 360 gaggcaggtt tactcggcgt tctcctcaca ggcgacgcct tcaacctctt cgtcatgata 420 gaagtcacca gcatcgcagc atacgccctc gtcatgttct acagggacag gggcgattca 480 atctgcgccg gcctgaagta cgcctttatc ggcgctctcg gaacgaccat gtacttcctc 540 gcgcttggga cactctacgg ggcgtttgga acattgaaca tggccaactt gagcgcatta 600 atccacggat tgagcttccc gatagcgggc accacttacg gcaatatcgc gatagcttca 660 ggcgttgccc tggccttggc cacctgggcg ttcctcataa aggccgcaat agtcccgaac 720 cacttctggc tcccagaagc acacccagca gcaccaagcc caatctcagc catactctca 780 ggactagtcg tcaacgtcgg tgtctactca ctcatcagat tcctctacac cgtctacggc 840 ggcgaagtca gcggttcgct cggtggggtt atccacgccc tcagcgttat cctgatagtc 900 ctgggtgcgg tttcggcact tttcggtgca ctcatgatga acgcccagcg cgacgtcaag 960 aagctcatcg cctactccac gataatgcac atgggctatc tggcgatggc ggttggcgtt 1020 ggaacccagc tggcacttca ggcagcggct ttccacatca ttaaccacgc aatcgccaag 1080 gccctcctct tcctcgcggc gggtgccttt atccacgcgg tcggctcaag ggacatctca 1140 gacctggcag gcctcggcag gcagatgccc gttgccacct tcggcctggc catagcaacc 1200 ctcagcctcg tcggaatccc accattcaac gtcttcttca gcaagttact gctcttcaac 1260 gccttcatgg aggagagccc tgccctggcc ctggtcctcg tgctgagctc tgtgattgca 1320 ctggtggctt acgttagagt gttccaggag atctggctcg gcaagcccag ggagaatact 1380 accatcaggg agcatggcag catgagcggg gttctgctga tactggcagt catctgcctg 1440 ctgcttggac tcttcgcacc atacatcatc gaacactaca ttaaccccgc tgtaagccag 1500 gccatggact acaggctcta catccagacg gcactggaat acgcggcaaa gctgaagatg 1560 ggcctttaa 1569 <210> 211 <211> 588 <212> DNA <213> Thermococcus onnurineus NA1 <400> 211 ttgattggag cggttctagc gggaggcagg tcaaagcgct ttggtggaaa caaacttctg 60 tacagaatcg acggaaagcc gttaatcctt catacaattg agagacttga atcagcgaat 120 gaaatagaag aggtcgtcat cgttgcctct cctgaaaacg ctgaaaagct gaaaaccttt 180 ggctatcagg ttcttgttga tgagcttctc gttggcccaa ttggaggaat atatactgct 240 ctaggcttag gggacgcttt cgtcacggca ggggacatgc cccgtatagt gcctgagttt 300 gtagattata taatcaagta cttccacaag agtaggaagg ctgtctgcgt tccgcgctgg 360 attaatgggc acatagaacc cctgcatgca gcatattcta tgggattctt ggacattatc 420 gaggagcaac tagctttgga agaatacatg ataaagagag ccatagaaaa agccaatgtc 480 tgctacttaa caatagaaaa agtccccttt gaatggagag aaagcttttt caacgtgaac 540 agaaaagatg atctgcgaaa actcggaatt cctgaacctt tggtttaa 588 <210> 212 <211> 507 <212> DNA <213> Thermococcus onnurineus NA1 <400> 212 atggcattta ttgccgcctt catctggtgc tacatcctct ggctggggct gacggcaggc 60 agcagcggac tattatggag cagcgaggaa ctaattgccg gtctggtgtt ctcggcggta 120 atagcataca ctaccaaagg cctcattggt gaaaaggcta gccgcttcct aaacccaata 180 aaatggtttg agttcatagt ttactccatc ggcccgctct tctggggcat ggtcaaggcc 240 aacttccacg tcgcttggct cgttataacc ggcaagataa ggccaggcat agtgcgcgtt 300 cctgttgacc tcgaaaacga cgcccagtac acaatcctga gcaactcaat aacactcaca 360 cctggaacgc tcaccatcga tgcctgtcct gaggagaagg ccctctacgt ccactggatt 420 gacattcccg agggacttga gaagcctgaa agctccgagc ctgtggctgg atctttcgag 480 aagtgggcga ggaggttggg aagatga 507 <210> 213 <211> 264 <212> DNA <213> Thermococcus onnurineus NA1 <400> 213 atgatagaac cagtgttctt ctactcagca ctgataataa tggtcggcgc attcatagcc 60 gtgttgaggg ttcttctcgg accaaccgtg ccagataggg tcgtgggagt tgataccctc 120 aacacgctgg tagtggccgg catggttctc ctgggggcgg cctatgacag gacgatatac 180 atcgacatcg cgatagtcta cgcccttctg agctacatag gaaccctcgc tatagcccgc 240 tacatgcagg ggggattgaa atga 264 <210> 214 <211> 372 <212> DNA <213> Thermococcus onnurineus NA1 <400> 214 atgaacaccg ttgattacgt tatctacgcc ttcctcgcga taaacataac gttcaacctc 60 ctgggaagca ttgcattaca cagatttccc gacgtataca caaggcttca cggggcaaca 120 aagtgcacaa ccttcgggac tatattcgcc gtcctagcgg tcgttgtcca ttccctctac 180 cggctcgacg ttaccggcga tccaaagtac ctccagatga cactccacag cgttgtagct 240 ctcatagcgc tcttactcac caacccgacc ggcgcacacg ccatagctaa ggccgctcac 300 ctgagcggcg ttaaacctgt caaagctgtc atcgacgcct acgaagggaa gctcgggggt 360 ggtgaggaat ga 372 <210> 215 <211> 267 <212> DNA <213> Thermococcus onnurineus NA1 <400> 215 atgaacccca tcacaattga ccagatcatt cagtttggga ttcttatcag cgttctcatt 60 accgcatatc tcacgatacg cctcagggac ttgctcagtg cggctctcgc atctgcagcc 120 atgagcctgc tcctaagttt ggagttctac atgctccatg cgccagacgt tgcgatagct 180 gaagcggctg ttggtgcagg cgttgttacg gccatagttg tgtatggaat cgctaaaaca 240 gagagatggg agcgtgaggg atcatga 267 <210> 216 <211> 297 <212> DNA <213> Thermococcus onnurineus NA1 <400> 216 atgaagagaa ccctcgcttt cctttcgctg ctgttcattc tcggagcgct gctttacata 60 gccaaccctg actacggcct caaatttggg ctgggtgggg atgactggaa aaacctccgc 120 tacactgacg actactatat cacccacggc gtggaagaag tcggtggtac aaacatcgtt 180 acagacatag tctttgacta ccgtggctac gatacccttg gagaggccac cgtcctgttc 240 acggccatcg ctggagccgt tgtgcttctc aggccctgga ggagggatga ggaatga 297 <210> 217 <211> 447 <212> DNA <213> Thermococcus onnurineus NA1 <400> 217 atgaccgatg atatgggact catcgttaaa accatggcga ggacaaccat tccactcatc 60 gggatattcg gagcctacgt ggtttcccac ggtcatctga cgccaggtgg tggcttccag 120 ggaggcgcca ccatagcggg agcgggcata cttttcctca tagcattcgg cctgaaagag 180 atgaagaagg catacaacaa gcacctctac tcagcccttg agggaatcgg cggacttgtc 240 ttcctaggcg cagcaatgct tggcataggc acggcgttct tctacaacat actctggaac 300 aacggcccaa tcctcaatgg tgagcccgga acgcttcttt cagctgggtt cctcccaata 360 atgaacctag ccgttggttt gaaggttttc actggcctcg tcagcgcgat ggctgccata 420 gcactctaca ggaggtggaa gtcatga 447 <210> 218 <211> 360 <212> DNA <213> Thermococcus onnurineus NA1 <400> 218 atgataccct tccagttcat cactgccttc ctaatgatag ctctgggaat atacgccttc 60 ctgtacaaga gaaacctcat caagctcatt ctcgcattgg acattataga ctccggcatc 120 cacttgctcc tcgtaagcct cggataccgc atggagctta acgagctccc caccgcgcca 180 atttacacag gctacgagac gctcaagagc cctatggttg gcccacttcc acaggccctc 240 gtgctcacga gcatcgtcat tggcgtctgt gttctggctc ttgcgatggc cctcacgata 300 aacgcctacc gccactacgg taccctcgac gttaacgccc tcaggaggtt gagaggatga 360                                                                          360 <210> 219 <211> 1599 <212> DNA <213> Thermococcus onnurineus NA1 <400> 219 atgaacgggg aagcgatact cccctacctc ataatcatcc cgctcttcgg agcgtttgcg 60 atgcccatag tgaaccttgc aggaaaaaaa gcgagagagg cctgggcagt cctcataagt 120 gcgctgacgt ttatcgtaag ttcggtgata ttctaccaca tctggaacac tggagaaatt 180 atagtctaca ccctcggtgc agagacacca ttcggccagg gggttgattt cccgattagg 240 atagtgtggg aagtcgacct tttcggcgca ataatggtgt tcattgtggc gtttgtctct 300 ctaatggcag taatctactc catcggctac atgaagcacg acaccggact cgacaaatac 360 tacacactca tactcatcct tgagctggga atgctcggca tagccataac cggcgacctg 420 ttcaacttct acgtcttcct cgaaatcatg agcatagcca gctatgccct cgttgccttc 480 aggaatgaca catgggaggg catcgaagcg ggcataaagt acatgttcgt tggctcgata 540 gccagtgctt tcgtgctcct cggcatagcc ctcctctacg gccagtacgg aaccctcaca 600 atggggtacc tcgcagttaa aatcgtgcag aatcccactg tcaccgccaa ggtcgcattg 660 gccctcttca tagcggggct gctctttaag agcggtgcct ccccagtcca catgtggctc 720 gccgatgcac accccgcggc acctagcagc atctcggcaa tgctctcggg tctagtcatt 780 aagatcggtg gaatctatgc gatagcgaga atactcttca gcatctacac tccaatggcc 840 cacagtatag tgcttgaagc atatctccaa ccccacatta atatgagcgc gataggatgg 900 atcattatca tttttgcgtg cttaacgctg ataattggca atgcaatggc agtcatccaa 960 acggatatga agagactgtt ggcctactct tccgtcggac agataggtta catactcctt 1020 ggcctcggaa taagtatagc cgcctacggg actcagacag gcgagatagc cctggcaggt 1080 gcaatctacc acactgtaaa ccacgcgctc atgaaggccc tcttgttcct cgttgcgggt 1140 gtcgtaatcc accagcttgg cacgaggaat ctgaacgagc tgagtggatt agcgaaaacc 1200 atgcccaaaa caacgtttgc atttcttata ggtgcagccg ctatagtagg aatgccccca 1260 ttgaacggct ttgccagcaa gtggctcatc tatgagagtt cggccatctt caatccaatc 1320 cttggtgcaa tagccatcat tggaacggca ttctgtacgg cagcctacgt cagggtgctc 1380 tacacgttct ttggtaggcc gagcgagaag gtgatggccg ccaaagaccc agaaacctca 1440 atgatgcttc caatgctcct gctcgtgctt gcaataatca tcatggggct cctcccgtgg 1500 cagataagcg acaaggtcat gatacctgca gctaggatgc tggagcagtt cagatttgag 1560 tacataaacg ctctaatgag ccttgttggg ggtgcgtga 1599 <210> 220 <211> 348 <212> DNA <213> Thermococcus onnurineus NA1 <400> 220 atgtttggct actgggacgc tctttacttt gtctttatct ttattatcgg cctcatcata 60 gcatggatgt tgaacgaatg ggccaagaag tctggaatgg gtacgagaga agctggtgat 120 ggcacgaaag tcttcatcag tggtgaggac ccggacaagg taatccccgg cttcgagcat 180 tacgaaggtt actacactgg caagaacgtc atgtggggtc tcacatacgc cctcaaaagg 240 ttcttcgccc tcctcaggaa cgagcacaca ggtctgctca ccgattacgt aagctacctg 300 ctcataacga cggctttcgt gctcggagtg atactgattt gggggtga 348 <210> 221 <211> 528 <212> DNA <213> Thermococcus onnurineus NA1 <400> 221 atgagcatca aggttcccgc tgaccagaat agaacaaacg gaaccacgag tgagcgcgag 60 atgctggaga agagaatagc ccagttgtgc cgcttcatag gaagatcacc ctgggtattt 120 cacgtaaaca gtggaagctg caacggctgt gacatcgaaa tcatagccgc cctgaccccg 180 cgctatgatg ccgagcgctt tggcgtcaag ctcgtgggaa gccccaggca cgctgatgtt 240 ctcttagtaa cagggccagt cacagaccag agccttgaaa gggtcaagct tgtctacgag 300 cagacaccag acccgaagat agtcatagca gtgggatcgt gccccactgg cggtagcgtg 360 ttctatgaga gcccattcac caatgcaccg ctgagcaaca tcattccggt tgacgtctac 420 gtgccaggct gtccaccaag gcccgaggcc atactctacg gcgtcgtttt ggcccttgaa 480 aagctggcta aaatcctgaa aggcgaagtt ccggagggtg aagagtga 528 <210> 222 <211> 570 <212> DNA <213> Thermococcus onnurineus NA1 <400> 222 atggctgatg ataacagaat catggagaat gttgataatg ttagagaacc aaccaaggaa 60 gatactgtcg ctgagaccat aaagagccgt ttccccaatg ctcacgtgga gatacgggag 120 aacaagtggg gaagaaagcg cgtctgggtg atcgttccac gagaagacta caaagcgctc 180 atgaagttcc tccttgaact cgacccagag gcccactatt cgataggaat agagcaggac 240 tacggggaag agataggcta tatgagccac atcctgctgc actacgacaa tgctccagca 300 gtctcactgc tcgttgatgt tagagtaccc aaagacgatc cagtaattcc cgatatcagc 360 gacatcttcc cgatagcact ccagtacgaa agggaagccg ctgagatgat gggcatagtc 420 ttcgaaggta tccccgacag tagaaggctt ttccttccgg acgacttccc agagggtatc 480 tacccgctca gactcgacga aaaaggcata ccagaagaga ttgtcaagaa cgccggacac 540 ccgtactacc tgaagggggg agataaatga 570 <210> 223 <211> 1281 <212> DNA <213> Thermococcus onnurineus NA1 <400> 223 atgaccaaga aggtcgagta ctggataaag ataccgttcg gcccaattca tcccggctta 60 gaggaacctg agaagttcat acttacgctc gatggcgaaa ggatagtcaa cgttgatgtt 120 aagcttggct acaatctacg tggcctgcag tggatagcat acaggagaaa ttacgtccag 180 ataatgtacc tcgcggagag gatatgtggt atctgttcgt tctcccacaa ccacacctac 240 accagagccg ttgaggaagc ggccggaata gaagtgccag agagggctga gtacatccgt 300 gccataatag gcgagctcga gagggttcac tcccacctgc ttaaccttgg tgtcctcggc 360 cacgacatag gctacgacac ggtccttcac ctcacatggc tggcacgcga gagggtcatg 420 gatgttcttg aagccatctc agggaaccgc gtgaactact cgatggtaac cataggcggt 480 gtgagaagag acatcgatga aaaagggaag cggctcattc ttgatatgat aaagtactac 540 aggagcataa tgcctcagat agaagaggtt ttcctccacg acccaaccat agaagcccgt 600 ttgagggact gtgcggtgat aagcaagcgc gtcgcccttg agcagggtgc agtgggaccg 660 actgccagag cttccggtct aaaggtcgat gccaggtgga gtgagaggct tggtgtttac 720 cctgacctag gagttaagcc agtgatgcca caggacgtta cgggagaaaa accgcacggt 780 gatgtattcg acagggcagc cgtaagaata ggagaaatat accagagcct cgacatgctc 840 gaacacgcac tagaccagat gccagagggt aagataaaga cattcccaaa ggacaacatc 900 ttggttgcca agctcaagat tatggttgac ggagagggaa tcggaaggta cgaggctcca 960 cgtggcgagc tggtacacta tgttcgcgga aagaaaggct ccgataaacc gctccgctgg 1020 aaaccaaggg agccaacttt cccgaacctc ttcgcagttg ccaagggtgt gacaggtgat 1080 caggtggcag acttcgtgct ggcagtggcc tcgatagatc cgtgcctgag ctgtacagac 1140 agggttgccg tagtacagga tggaaagaag agaattctta ctgaaaccga cctgctgaga 1200 ctctcaataa agaagacacg cgagataaac cccgaagtta aaggcgaccc aacaccggtc 1260 ggcttcggct gctcgaggtg a 1281 <210> 224 <211> 993 <212> DNA <213> Thermococcus onnurineus NA1 <400> 224 atggacgtaa tggcgaacat catttatccg gtagcaggtt taataggcct ttacgctttc 60 gtctcactgg catcgctcgt ctgggaaggt atagacagaa agctcgtcgc aaggatgcag 120 agaagggtag gaccgccgct tctccagccc ctctatgact tcttcaagct agcgagcaag 180 gaaacaataa tccccaacac ggctaacttt atgtttagag ccgcacctgt actcgccctg 240 gcaacggcca tagcactcct cgcttacacc ccgatgggct ttgctccact actcgcgagc 300 aagggagacg tcatcgtttt catatatctc ctcaccctca ttggcttctt caagatactc 360 ggtggcataa gctcaggaag cccctacgca aagataggag ctgcaaggga agcagcaata 420 atggtttcca gagagcctgc catgatgctg gccctattcg ctataatatg gcgtcttgga 480 aaactcggag tcaacaagcc attcagcatg gaggtcttct accagtacaa catttgggaa 540 ataggtaccc cgctcagcct tataggtgcc gtaatcctcc tttacgtctt cgtcatttgg 600 ctggcaagtg aaatagaagt cggatatttc aacatacccg atgcagagga ggagatagcc 660 gagggactgc tcgccgagta cagcgggcgc tacctggccc tgttaaagct cacgaaggca 720 ctgaaaactt acatagcagc atcgctcgtc gtagcaatat tcttcccctg gggaatagca 780 gattacttca acctcaccgg acttccagca aacgtcgtaa acctgctctt ccatacactc 840 aaggtattca tactgctctt tgctgtgcag agtgtcttca gggccactac aggcagactc 900 aaaataacgc aggcggttga cttcctctgg aagaacgtct tcttagcttc gctcatcggc 960 acactcctta tcgccatgga ggtgataatg tga 993 <210> 225 <211> 459 <212> DNA <213> Thermococcus onnurineus NA1 <400> 225 gtgaggctct ccccactcat ccccaccgtg ctcagaaaca tgttcaaaaa gcctgccacc 60 aacctctttc ctgcgactga accagtgccg gttccagata acttcagggg ccagctgaag 120 tacaacgtgg acaagtgtgt cggctgcagg atgtgcgtca cagtctgtcc agccggcgtc 180 ttcgtcttcc tacctgagat aaggaaggtc gctctgtgga ccgctagatg tgtctactgc 240 tcgcagtgtg ttgacgtttg tccgaccgca gccctccaga tgagcgatga gttcctgctg 300 gcaagctaca acaactacga cgacaagttc atcccgctca agcccgaaaa ggttgaagag 360 ataaagaaga aactggagga gcaaaagaaa gcgaaggcag ctgcagctgc caagaaagcc 420 atggagaaaa aggaagcagg gaaagaggcc aaaaagtga 459 <210> 226 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 226 ggaccatgta gaatcgaycc gtty 24 <210> 227 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 227 ttcrtttccg gtacagca 18 <210> 228 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 228 atggcacagc ttagtggaca g 21 <210> 229 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 229 caaggatttc ctgggctttc tc 22  

Claims (8)

Hydrogenase consisting of any one amino acid sequence selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 112. Gene encoding the hydrogenase of claim 1. The gene encoding a hydrogenase according to claim 2, wherein the gene comprises any one nucleotide sequence selected from the group consisting of SEQ ID NOs: 113 to 225. Thermococcus spp. Method of producing hydrogen by culturing. The method of claim 4, wherein the Thermococcus spp. Is Thermococcus onnurineus NA1 (Accession No .: KCTC 10859BP). 6. The method of claim 4 or 5, wherein said culturing is at a high temperature of 80 ° C. The method for producing hydrogen according to claim 4 or 5, wherein the culturing is carried out by supplying a medium and carbon monoxide made under anaerobic conditions. 6. The method of claim 4 or 5, wherein said culturing is in a medium to which formate has been added.

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