KR20090008829A - Attraction trap of stink bugs using live stink bugs for determination of chemical communication reaction within intra and intra species of stink bugs - Google Patents

Abstract

An attraction trap for checking chemical communication reaction of stink bugs is provided, which effectively secures the practical experiment materials for the pheromone development. An attraction trap comprises: a front side sliding door and a ceiling sliding door(6'); a quadrangular pyramid(3) of which both sides are formed at the inside; a trigger unit(4) formed in the corner of the quadrangular pyramid; and a drain(10) installed at the floor side. A hole(5) is punched on the plane of the quadrangular pyramid at regular interval. On the floor side of the trap for capture, a support stand(9) is installed.

Description

Attraction trap of stink bugs using live stink bugs for determination of chemical communication reaction within intra and intra species of stink bugs}

The development of communication compounds for insects is largely subject to the separation, identification, preparation and synthesis of volatile attractants and the bioassay process that assesses the attractiveness of the target insect using potential attractants. Nucleic acid is used to make crude extracts from target insects, and precise analyzers such as GC-EAD and GC-MS are used to prepare potential attractants, ie communication compounds, that can cause behavioral changes in the target insects. . Evaluation of whether the prepared communication compound has a attracting force or a repelling effect on the target insect is finally evaluated through bioassay in the room and the package. In-situ assays can be used to precisely test the behavioral response of a target insect to an assay using wind-tunnel and Y-tube olfactometer methods. Even if the indoor bioassay showed a positive inducement reaction, the final judgment would be to determine the type, function and possibility of practical use of the communication compound through the inducement reaction in the package. Therefore, the test of chemical communication of stink bugs using the biostrap of stink bugs using the stink bug, which is not directly prepared or synthesized and prepared from synthetic insects, as a attractant, is necessary for the development of communication compounds of the target insects. Deterministic information can be obtained early. When the types of insects attracted to the biological trap of the present invention are classified by sex, the types, functions, and timing of the communication compounds emitted by the target insects can be evaluated very quickly and effectively at the crop cultivation site. In other words, it can greatly contribute to the early development of pheromones and heterologous sensitive substances.

 Information transmission of insects using communication compounds, ie, pheromones, is widely used in agricultural sites by predicting the occurrence of target insects in the ecosystem, density estimation, mating disturbance, and mass capture. Field. Insect communication compounds do not apply to all insects, but rather act as important means of information transfer between species and species in limited insects such as lepidoptera, stink wood, coleoptera and felling. It is a method of information exchange between individuals causing unique behavioral changes. Diagnosis of the release of communication compounds in certain insects and the analysis of behavioral and physiological reactions between homogeneous and heterogeneous individuals that accept volatile communication compounds released from an individual, as well as the chemistry of ecological and material analysis Requires precise analysis The test of the chemical communication of stink bug by biotrap using the stink bug of stink bug is to directly test the multiplying stage of insects in the cultivation and packaging of crops. Can be tested. Therefore, investigating the species and sex of insects attracted to the biotrap of stink bugs can effectively obtain very important information on the type, function, and timing of chemical communication of stink bugs. It can greatly contribute to the early development of materials.

The present invention is a biotrap that uses the target insects living in the multi-generation of the target insects as a bait in the crop cultivation field to test the attraction to homogeneous and heterogeneous species by releasing volatile communication substances while the insects live in the box in the trap. Practical experimental data for the early development of pheromones of the target insects can be obtained very effectively at farm sites. Therefore, the chemical communication of insects using the biotrap of stink bugs is not only useful for the development of pheromones involved in the transmission of information in the target insect species, but also in the development of heterologous substances such as Cairo, which are involved in the information transmission between species. It can be actively used and consequently can be used for early development of insect communication compounds.

First, by using the raw chap trap of the stink bugs of the present invention, it is possible to effectively test the type and function of the communication compound acting between the same stink bugs. When different types and genders of stink bugs are injected into the biotrap and installed in the crop production package of the insect insect, if the live stink bug injected into the biotrap emits volatile communication materials, the same kind of recipients who recognize the volatile communication materials It is attracted to the trap. Investigating the sex and stage of development of homologous stink bugs attracted to the biotrap can provide data to determine the type, function and practicality of the communication compounds of stink bugs. Second, it is possible to effectively test the type and function of the communication compound acting between different kinds of stink bug, and when the stink bug living in the bio trap emits a volatile communication material, the heterogeneous recipient who recognizes it is attracted to the bio trap. Investigating the sex and developmental stages of the dissimilar stink bugs provides data that can be used to determine the types and functions of the stink bugs and their usefulness. Third, the analysis of the presence or absence of the communication compound according to the sex and the number of days after allegory of the stink bug living in the biological trap, and the attracting amount of homologous and heterologous insects according to the release were analyzed. By systematically analyzing the ecological behavior and physicochemical properties of stink bugs at this stage, it is possible to examine the types, functions and functions of the communication compounds emitted by stink bugs injected into the biotrap. Information can be effectively obtained. Fourth, the biotrap can be easily applied to the test of the chemical communication action in other insects such as lepidoptera, coleoptera, felling, as well as the test for the presence or absence of the communication compound action of stink bugs. In addition, the interaction between the species and the application of species to the insects can be investigated. In particular, the attraction and repelling effects on the natural enemies of the insects are examined. It can be developed as a repellent and used as an eco-friendly management technology for natural enemies of insects, including stink bugs.

The biological trap of the present invention is composed of a trap for attracting insects (1) and a breeding box (2) of a production insect. The trap 1 is a rectangular box, the left and right sides of which form square pyramids 3 suitable for attracting insects into the trap, respectively, and the vertex portions of the square pyramids form square inlets 4. The material is a transparent synthetic resin product such as film, acrylic, plastic, etc., which can observe the inside of the bio trap, and a mesh made of metal or synthetic resin for controlling air flow and temperature in the trap. Each side of the square pyramid 3 of the trapping trap drills the holes 5 at regular intervals for the response of the communication compound. Sliding doors 6 and 6 'are placed on the front and the ceiling of the trap 1, respectively. One side of the sliding door forms a door ring (7) (7 ') and the other side has a handle (8) (8'). Sliding door 6 of the front punctures the center portion and the perforated portion is attached to the mesh 31 to function to regulate the air flow and temperature in the trap. Meshes 32 and 33 are also attached to the ceiling and the rear surface of the trap 1 for capturing the air flow and temperature. The bottom surface of the trap 1 is composed of a support 9 and a drain 10 for supporting the breeding breeding box 2, and a mesh 11 is installed at the drain to the bottom of the trap 1 during rainfall. Do not allow water to collect. Rings 12, 12 ', 12 " and 12 "' are respectively provided on the outer corners of the ceiling of the trap 1 to allow the trap 1 to be walked at any place.

Production larvae breeding box (2) is divided into female and male adult hens, males and males to breed 10 to 20 hens of the same species of hens. The production worms release the volatile communication material while ingesting food and water in the breeding box 2 and the same and different stink bugs and natural enemies are attracted into the trap 1 for capture. Each side of the breeding box 2 imparts transparency and breathability to allow the larvae to breed in an optimal environment. As a transparent material, a transparent synthetic resin such as film, acrylic, plastic, or the like is used, and ventilators 21 and 22 are disposed on the ceiling and the side to attach air to the outside and control the temperature. The front has a sliding door 23 to function as an entrance and exit of the generated insect. Sliding door 23 is attached to the mesh to be ventilated, and also install the handle 25. The right side of the ceiling is built with cotton and the lid 29 is attached to the bucket 26 is installed so that the insects absorb moisture. Inside the bottom surface to create a bottom vent (30) and feeders (27) to contain soybeans, peanuts. Each of the four corners of the bottom outer surface has a support protrusion 28 so that the production insect breeding box 2 is not submerged in water during rainfall.

<Example 1>

The trap 1 is made of transparent acrylic in a rectangular box of 55 × 22 × 22 cm. The sliding door is provided with a front sliding door 6 of 15.5 × 15.5 cm and a ceiling sliding door 6 'of 12 × 15.5 cm. Both sides form a 12-cm-deep square pyramid (3) inward, and the vertex portion of the square pyramid forms a square 1 × 1 cm inlet (4), and on each side there are 76 holes (5) at regular intervals. Perforate. One side of the sliding door forms a door ring (7) (7 ') and the other side has a handle (8) (8'). Sliding door 6 of the front perforated the center portion and the perforated portion attaches the wire mesh 31. Wire meshes 32 and 33 are also attached to the ceiling and the rear surface of the trap 1 for capturing air flow and temperature. The bottom surface of the trap 1 is provided with a support 9 for supporting the breeding box 2 and four drain holes 10 having a diameter of 3 cm, and a stainless steel wire net 11 for the drain hole. Attach. Rings 12, 12 ", 12" 'and 12 "" are provided on the four corners of the outer ceiling of the trap 1 for producing the trap 1 for capture.

<Example 2>

The breeding box 2 is made of transparent acrylic in a rectangular box of 15 × 12.5 × 12.5 cm. Each side is air permeable with transparency and steel mesh so that the insects can be reared in an optimal environment. The ceiling and sides have wire mesh vents 21 and 22 of 7 × 8.5 cm and 9 × 9 cm. The front face has a wire mesh sliding door 23 having a size of 12 × 8 cm to serve as the entrance and exit of the generated insect. On the right side of the ceiling is installed a bucket 26 with a cotton 50ml capacity, 12cm in length, 2.8cm in diameter so that the generated insects absorb moisture. 4 × 4 ㎝ feeder (27) is made at each corner inside the bottom to contain soybeans and peanuts. The bottom of the feeding container is drilled through 12 holes 34 of 2 mm to drain the water and a wire mesh ventilation opening 30 having a diameter of 5 cm. The bottom four corners of the outer surface to produce a production insect breeding box (2) each having a support projection (28).

<Example 3>

The biotrap of the present invention was installed in soybean and sesame packages, and the stink bug adult insects were injected as shown in Table 1, and the intra- and inter-species interaction tests of stink bugs were carried out by means of the stink bugs attracted to the bio-traps.

 Intraspecies and Interspecies Interaction Test of Stink bugs using Stink Bug Biotrap Injecting rinsing material for biological trap Attracted Water (Marine) / Straw Chaps by Kind of Stink Bugs Sawtooth Ant Loin Horizontal row Vines Alkali bug Full color Star gender female cock female cock female cock female cock female cock Sawtooth Ant Loin Female male 1 91 10 71 1 10 3 0 0 0 0 1 0 0 0 0 0 0 0 0 Horizontal Line Female male 0 0 0 2 0 9 3 2 0 0 2 0 0 0 0 0 0 0 0 0 Locust stink Female male 2 0 3 1 2 in 1 0 3 1 4 4 1 1 0 0 0 0 0 0 0 Alkaline norin Female male 0 4 0 2 2 0 1 3 0 1 0 0 0 7 0 5 0 0 0 0 Full color stink bug Female male 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 3 0 3

※ Investigation period; Sawtower, vine, algae sapling (8/23 ~ 11/11), horizontal line (8/23 ~ 10/31), full-colored sapling (9/24 ~ 11/11)

The biotrap of the present invention can be easily applied to the assay of the chemical communication action in other insects such as lepidoptera, coleoptera, felling, as well as the test of the presence or absence of a communication compound action of stink bugs. In addition, the interaction between the species and the interspecies action of the target insect can be investigated, and in particular, the attracting effect and the repelling effect on the natural enemy of the target insect are examined. It can be developed as a repellent and used as an eco-friendly management technology using natural enemies against insects including stink bugs.

1 is an exploded perspective view of a living body trap,

2 is a perspective view of a trap for trapping attracted insects,

3 is a perspective view of the sliding door separated from the trap;

4 is a front view of the incision of the living body trap,

5 is a breeding box for breeding the production larva by separating the dominant female and male adult,

6 is a perspective view showing the bottom surface of the production insect breeding box.

Claims (7)

The trap 1 is a rectangular hexahedron with a front sliding door 6 and a ceiling sliding door 6 '. ), The bottom surface is trapped by the installation of a drain (10) (1). The method according to claim 1, wherein each square pyramid perforates the holes (5) at regular intervals. Sliding doors (6) on the front, and meshes (31, 32, 33) are attached to the ceiling and the rear, respectively, and the bottom surface of the trap (1) is a support (9) for supporting the breeding box (2). Capture traps (1). Producer breeding box (2) is a rectangular hexahedron, the bucket (26) is installed on the right side of the ceiling, the feeder (27) and the bottom ring mechanism (30) inside the bottom surface, each of the four corners outside the bottom surface Producer rearing box (2) having a support projection (28). 4. The carnivore breeding box (2) according to claim 3, wherein the ceiling and sides of the breeding box (2) have ventilation openings (21) and (22), and the front side has a sliding door (23) with a mesh attached. A living trap consisting of the trap (1) of claim 1 and the breeding box (2) of claim 3. A method for assaying the type and function of a communication compound acting between homogeneous stink bugs using the biotrap of claim 5. A method of attracting allogeneic and heterologous larvae by the larvae bred using the biotrap of claim 5.

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