KR20080086641A - Composition comprising rubus crataegifolius leaves extract for inhibiting obesity and preventing or treating hyperlipidemia - Google Patents

Abstract

A composition comprising the extract of Rubus crataegifolius leaf is provided to reduce lipid tissue weight, serum lipid, serum total cholesterol and low density lipid cholesterol, increase high density lipid cholesterol, and decrease augmentation index(AI) for arteriosclerosis, so that the composition is useful for inhibiting obesity and preventing or treating hyperlipidemia. A pharmaceutical or health food composition for inhibiting obesity and preventing or treating hyperlipidemia comprises the extract of Rubus crataegifolius leaf which is prepared by sequentially extracting and isolating the methanol extract of Rubus crataegifolius leaf with hexane, ether and butanol, sequentially, wherein the daily dosage of the Rubus crataegifolius leaf extract is 500-1,500 mg/kg, preferably 600-1,200 mg/kg.

Description

Composition comprising Rubus crataegifolius leaves extract for inhibiting obesity and preventing or treating hyperlipidemia

The present invention relates to a composition for inhibiting obesity and preventing or treating hyperlipidemia comprising raspberry leaf extract.

There are many changes in eating habits due to recent economic growth and lifestyle changes. In particular, busy modern people are gaining weight and obesity due to high calorie diets such as fast food and low exercise.

Overweight means a body mass index (BMI, an obesity measure of body weight divided by the square of height) of 25 or more and less than 30. Obesity is applicable when the body mass index is 30 or more.

Two-thirds of American adults are overweight or obese, and overweight increases blood pressure and cholesterol levels, increasing the incidence of various adult diseases, including heart disease, and obesity increasing the risk of heart disease, diabetes, arthritis and some cancers .

Overweight and obesity have contributed to increasing the incidence of various adult diseases such as arteriosclerosis, hypertension, hyperlipidemia or heart disease in children as well as adolescents.

As such, obesity affects all age groups, but the incidence of older adults is high. It is known that obesity was not so much in the East because of vegetarian-oriented lifestyle, but in modern times, obesity was gradually increasing in Asian people as the dietary habits changed and the living environment changed due to the influence of western culture.

In addition, modern man has been reported that physical strength is reduced due to overwork, heavy drinking, stress, etc., about 30-40% have obesity, about 10% of them have severe or pathological obesity has been reported.

Obesity refers to a state in which fat is excessively accumulated in the body and a high proportion of fat in the body weight is classified into simple obesity and secondary obesity (symptomatic obesity).

The causes of simplicity obesity include overeating, lack of exercise, and decreased metabolism. The majority of cases that are clinically judged as obesity are simplicity obesity. If obesity manifests and the condition persists, it causes various health disorders.

Secondary obesity is obesity caused by any underlying disease, and may include endocrine obesity, hypothalamic obesity, hereditary obesity, or obesity due to drugs.

The main cause of obesity is overgrowth of fat cells, which is caused by a deficiency of a hormone called leptin, which is ordered and secreted by the brain. The deficiency of leptin hormone is reduced by overeating, weakening of metabolism in vivo due to aging, and animal cholesterol accumulated in the body, except when genetic deficiency occurs.

Cholesterol is present in the blood in the form of small round particles called lipoproteins. There are many types of lipoproteins, LDL (low density lipoprotein) that carries cholesterol from the liver to other tissues, and HDL (lipid protein) that brings cholesterol from the liver to other tissues. high density lipoprotein). HDL-cholesterol, in particular, cleans blood vessels and lowers the risk of atherosclerosis. Therefore, low HDL-cholesterol levels increase the risk of atherosclerosis, as do many bad LDL-cholesterols.

Obesity itself is the cause of various diseases such as hyperlipidemia, hypertension, cardiovascular disease, pseudotumor cerebri, sleep apnea, cancer, pulmonary hypertension, cholecystitis and osteoarthritis Or cause problems such as deterioration of activity. In addition, obesity may be caused by various diseases and may appear as a complication.

Hyperlipidemia is one of the most common diseases caused by obesity. Hyperlipidemia generally refers to a case in which the blood cholesterol level is 240 mg / dL or more, and is recommended to be lowered below the normal level of 200 mg / dL. The causes of hyperlipidemia are heredity, alcohol consumption, high fat and high calorie meals, obesity, diabetes, liver dysfunction, thyroid dysfunction and nephrotic syndrome.

Drug therapy for the treatment of obesity currently being used include an appetite suppressant, digestive absorption inhibitors, obesity-inhibiting inhibitors or metabolic accelerators, these obesity drugs have a risk of causing side effects such as drug dependence. In addition, there is a problem in that resistance can be developed in a short period of time to a patient to be administered, so that it cannot be used continuously for a long time.

In addition, as a treatment method commonly used to treat hyperlipidemia, treatment through diet is preferred, but if it is not improved, bile acid-binding resins, nicotinic acid derivatives, and fibric acid induction Reagents such as retention and probucol have been used. Recently, the most commonly used HMG CoA reductase inhibitors and the like are used as therapeutic agents, but these drugs have not yet obtained a clinically satisfactory effect.

Therefore, many researchers have been using the herbal medicines to develop anti-obesity drugs and hyperlipidemia drugs that are nontoxic and have no side effects even after long-term use.

On the other hand, the raspberry (Rubus crataegifolius) is a plant belonging to the genus Rousaceae (Rubus), the thorns are many, the stem is reddish brown, and the leaves are shifted single leaves. Raspberries are delicious, warm in nature and nonpoisonous. Raspberry starch contains flavonoids and roots contain triterpene saponins as main ingredients. Other organic acids, malic acid and vitamin C are also included.

In general, the plants of the genus Lubus have been used for medicinal fruits, roots and leaves, in particular, the fruits of the genus Rubus have been used in the civilian to treat tonic, cooling, jigging and diabetes. In addition, the leaves are effective for asthma, the roots are known to be effective for hemostasis, hematopoiesis, infertility, menstrual irregularities.

The unripe fruit of raspberries is called bokbunja (Rubus coreanus, Rosaceae). Bokbunja is used for tonic, sonic, branch, and concubines (Moon G.S: Constituents and Uses of Medicinal Herbs, Ilweolseogak, Seoul, 1991, pp. 310-311). In addition, the inventors have already reported that Bokbunja has not only anti-inflammatory, analgesic and anti-arthritic effects, but also long-term administration of gastrointestinal damage in rats and gastric protective effects in rats that induce gastritis. (Nam JH, Jung HJ, Choi J, Lee KT and Park HJ. 2006.The antigastropathic and anti-rheumatic effect of niga-ichigoside F1 and 23-hydroxytormentic acid isolated from the unripe fruits of Rubus coreanus in a rat model, Biol Pharm.Bull. 29, 967-970.

The fruits of Bokbunja become red when ripe and black when fully cooked, whereas the fruits of raspberries ripen red and do not black. Therefore, the fruit of Bokbunja is called blackberry in Korea, and the fruit of raspberry is called red raspberry.

The present inventors compared the components of triterpenoids of immature fruits and ripened fruits of the genus Lubus, and found that immature fruits contained a large amount of triterpenoids, but contained only a very small amount in ripened fruits. Found. In addition, a number of 19 alpha-hydroxyursane-type triterpenoids are known in the plant of the genus Rubus, and typical compounds thereof include eucaphic acid and niga-ichigo. Niga-ichigoside F1, tormentic acid, 23-hydroxytormentic acid and derivatives thereof have been reported. Among these compounds, Niga-Ichigoside F1 and 23-hydroxytormentinic acid have analgesic anti-inflammatory effects, such as rheumatoid arthritis, pain (acute pain, chronic pain, inflammatory pain, neuropathic pain, postoperative pain, migraine, joint pain) And inflammatory diseases.

The anti-inflammatory effects of alcoholic extracts of raspberry roots (Cao Y. et al., Zhongguo Zhong Yao Za Zhi, 21 (11): 687-8, 1996). Anticancer Effect of Extracts (Lee JH et al., Arch Pharm Res., 23 (4): 338-43, 2000), and Antimicrobial Effects of Ethanol Extracts from Raspberry Roots (Korean Patent Publication No. 10-2006-84263) Etc. are known.

As described above, many studies on the physiological activity of the berries and roots of raspberries have been conducted, but there are almost no studies on the physiological activities of the leaves of raspberries. In addition, the anti-obesity and anti-hyperlipidemic effects of raspberry leaves are not yet known.

Moreover, the fruits of raspberries are difficult to collect in large quantities and are very expensive, while the leaves of raspberries are rarely used and disposed of.

Thus, the present inventors obtained a high-purity fraction by extracting the leaves of wild strawberry, it was confirmed that the fraction contains about 10 kinds of 19 alpha-hydroxyuric acid-type triterpenoid compounds. In addition, administration of this fraction in rats fed a high-fat diet significantly reduced the weight of adipose tissue, serum lipids, serum total cholesterol and low-density lipid cholesterol, significantly increased high-density lipid cholesterol, and increased risk of atherosclerosis. AI) was also significantly reduced, and the present invention was completed.

The present invention is to provide a composition for inhibiting obesity and preventing or treating hyperlipidemia comprising raspberry leaf extract.

The present invention provides a composition for inhibiting obesity and preventing or treating hyperlipidemia comprising raspberry leaf extract.

Compositions according to the present invention include pharmaceutical compositions and food compositions.

Hereinafter, the present invention will be described in detail.

Raspberry leaf extract according to the present invention is prepared by extraction and separation in the following manner. First, the leaves of raspberries are added to water, alcohol or a mixed solvent of water and alcohol to extract reflux (3 times). The extract was filtered, concentrated under reduced pressure, and lyophilized to obtain a raspberry leaf extract in powder form. The mixed solvent of water and alcohol may be selected from 10 to 100% methanol or 10 to 100% ethanol, preferably 10 to 100% methanol.

The raspberry leaf extract is suspended in distilled water and extracted three times with hexane. Then, the remaining aqueous layer was repeatedly extracted three times with ether, and the remaining aqueous layer was extracted three times with saturated butanol, and the butanol layer was concentrated. The butanol fraction is suspended in distilled water, activated charcoal is added, shaken, and filtered with a filter paper. The filtrate was placed in a Diaion HP-20 (320 g, 5 × 7 cm) column and developed with distilled water to remove sugar fractions. Then, the filtrate was further developed with methanol to collect the effluent, and the effluent was concentrated under reduced pressure to obtain a raspberry leaf butanol fraction. Get

Among the leaves of the genus Lubus plant, components such as diterpene, tannin, flavonoids, etc. have been reported in addition to the triterpenoid compound. However, extraction by the extraction method according to the present invention described above enables the removal of nonpolar diterpene present in the nonpolar solvent fraction, the removal of phenolic substances through the activated carbon column, and the HP-diaion column. It is possible to remove the salts and sugars through the high purity raspberry leaf butanol fraction of high purity is possible.

The raspberry leaf butanol fraction according to the present invention contains 19 alpha-hydroxyuric acid-type triterpenoid compounds, that is, a pomolic acid ester (28-O-formyl-3,19-dihydroxyurs-12-en-). 28-oate), use car piksan (euscaphic acid), tor men tinsan (tormentic acid), 23- hydroxy-Men tinsan Torr (23-hydroxytormentic acid), Kaji-Ichigo side _{F 1 (kaji-ichigoside F 1} ), Rosa 10 such Ichigo side F ₁ and _{F 2 (niga-ichigosides F 1} and F 2), Suarez BC base side F ₁ (suavissimoside F ₁₎ and collection cyano-side F ₁ (coreanoside F ₁₎ - multin (rosamultin), it's Includes female species.

Raspberry leaf extract according to the present invention significantly reduces the weight of the celiac adipose tissue, epididymal adipose tissue and total peritoneal adipose tissue of rats bred in a high fat diet, significantly reduces serum total lipid, phospholipids and triglycerides, Significantly decreases serum total cholesterol and low density lipid cholesterol, significantly increases high density lipid cholesterol, and also significantly decreases the artherogenic index (AI). Therefore, raspberry leaf extract according to the present invention can be used as a medicine and health food useful for inhibiting obesity and preventing or treating hyperlipidemia.

The composition of the present invention may contain at least one known active ingredient having an effect of inhibiting obesity and preventing or treating hyperlipidemia with raspberry leaf extract.

The composition of the present invention may be prepared by including one or more pharmaceutically acceptable carriers in addition to the above-described active ingredients for administration. Pharmaceutically acceptable carriers may be used in combination with saline, sterile water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol, and one or more of these components, as necessary. And other conventional additives such as bacteriostatic agents can be added. Diluents, dispersants, surfactants, binders and lubricants may also be added in addition to formulate into injectable formulations, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like. Furthermore, it may be preferably formulated according to each disease or component by a suitable method in the art or using a method disclosed in Remington's Pharmaceutical Science (Recent Edition), Mack Publishing Company, Easton PA.

The composition of the present invention can be administered orally or parenterally (eg, applied intravenously, subcutaneously, intraperitoneally or topically) according to the desired method, and the dosage is based on the weight, age, sex and health of the patient. The range varies depending on the diet, the time of administration, the method of administration, the rate of excretion and the severity of the disease. The daily dosage of the raspberry leaf extract is about 500 to 1,500 mg / kg, preferably about 600 to 1,200 mg / kg, and more preferably administered once to several times a day.

The composition of the present invention can be used alone or in combination with methods using surgery, hormonal therapy, drug treatment and biological response modifiers for the inhibition of obesity and the prevention or treatment of hyperlipidemia.

The composition of the present invention can be added to health food for the purpose of inhibiting obesity and improving hyperlipidemia. When the raspberry leaf extract of the present invention is used as a food additive, the raspberry leaf extract may be added as it is or used with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixed amount of the active ingredient may be suitably determined depending on the purpose of use (prevention, health or therapeutic treatment). In general, in the preparation of food or beverage, the raspberry leaf extract of the present invention is added in an amount of 15% by weight or less, preferably 10% by weight or less with respect to the raw material. However, in the case of long-term intake for health and hygiene or health control, the amount may be below the above range, and the active ingredient may be used in an amount above the above range because there is no problem in terms of safety. .

There is no particular limitation on the kind of food. Examples of the food to which the substance can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, drinks, Alcoholic beverages and vitamin complexes, and includes all health foods in the conventional sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates, etc. as additional components, as in the general beverage. Natural carbohydrates described above are glucose, monosaccharides such as fructose, malsaccharides, disaccharides such as sucrose, and polysaccharides such as dextrin, cyclodextrin, sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetening agent, natural sweetening agents such as tautin and stevia extract, synthetic sweetening agents such as saccharin and aspartame, and the like can be used. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohols, carbonic acid. Carbonating agents and the like used in beverages. In addition, the composition of the present invention may contain a pulp for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components can be used independently or in combination. The proportion of such additives is not critical but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, preferred examples are provided to aid in understanding the present invention. However, the following examples are merely provided to more easily understand the present invention, and the contents of the present invention are not limited by the examples.

Example  One  : Preparation of Raspberry Leaf Extract

The leaves of raspberries were collected in June in Sanya, Wonju, Gangwon-do, and the leaves were shaded and shredded. 2.4 kg of sliced raspberries were placed in a round bottom flask, and 3 L of methanol was added thereto, followed by extraction three times for 5 hours under reflux. The extract was filtered, concentrated under reduced pressure using a vacuum concentrator, and lyophilized to obtain 156 g of raspberry leaf methanol extract in powder form. 150 g of the raspberry leaf methanol extract was suspended in 1 L of distilled water, and extracted three times with 0.8 L of hexane. The remaining aqueous layer was then partitioned and extracted three times with 0.8 L of ether each time. The remaining aqueous layer was extracted three times with 0.8 L each of saturated butanol, and the butanol layer was concentrated. The butanol fraction was suspended in 0.8 L of distilled water, 70 g of activated carbon was added thereto, shaken, and filtered with a filter paper. The filtrate was placed in a Diaion HP-20 (320 g, 5 × 7 cm) column and developed with 2.0 L of distilled water to remove the sugar fraction, followed by developing with 2.0 L of methanol to collect the effluent. The effluent was concentrated under reduced pressure to obtain 18 g of raspberry leaf butanol fraction (19α-HUT fraction).

Experimental Example  One  : Anti-obesity  And antihyperlipidemic effects

In order to determine the effect of raspberry leaf extract according to the present invention on the anti-obesity and anti-hyperlipidemic effect, the following experiment was performed.

1. Experimental Animal

Experimental animals were fed Sprague-Dawley male rats weighing 190 ± 10g from Hyochang Science under constant conditions of animal history (temperature: 20 ± 2 ℃, humidity: 40 ~ 60%, contrast: 12 hours during the day and night). 2 weeks under light / dark cycle). 24 hours before the start of the experiment only watered and fasted. At this time, the experimental animals were treated within a certain time (10:00 AM ~ 12:00 AM) in consideration of the daily variation of enzyme activity. The experimental animals were divided into 4 groups and 6 animals were allocated to 1 group.

2. Obesity and hyperlipidemia induction, and sample administration

After one week of adaptation of the experimental animals, one group was fed a normal diet as a normal group, and three experimental groups were freely fed a preparation sample containing wedges for 6 weeks for obesity and diet. Induced hyperlipidemia. The preparation sample is shown in Table 1.

Normal and high-fat diet groups were administered with 1 ml of saline, and two experimental groups were dissolved orally dissolved 30 mg / kg or 60 mg / kg of the raspberry leaf butanol fraction prepared in Example 1 in 1 ml of saline. Oral jonde was used for oral administration to experimental animals for 2 weeks.

1) normal group,

2) high fat diet,

3) high fat diet group + 30 mg / kg of raspberry leaf butanol fraction prepared in Example 1,

4) high fat diet group + 60 mg / kg of raspberry leaf butanol fraction prepared in Example 1.

ingredient General dietary High fat diet Casein 20.0 20.0 DL-Methionine 0.3 0.3 Corn starch 15.0 15.0 Sucrose 50.0 34.5 cellulose 5.0 5.0 Corn oil 5.0 - AIN-mineral mixture ^{1 )} 3.5 3.5 AIN-vitamin mixtures ^{2 )} 1.0 1.0 Choline bitartrate 0.2 0.2 Beef tallow - 21.5

※ 1) AIN-mineral mixture (g / kg diet): 200.0 g / kg dicalcium phosphate dibasic, NaCl 74.0 g / kg, potassium citrate monohydrate 220.0 g / kg, potassium sulfate sulfate 52.0 g / kg, magnesium oxide 24.0 g / kg, magnesium carbonate 3.5 g / kg, ferric citrate 6.0 g / kg, zinc carbonate 1.6 g / kg 0.3 g / kg of cupric carbonate, 0.01 g / kg of potassium iodate, 0.55 g / kg of potassium potassium sulfate, sucrose, and 1,000 g / kg of fine powder.

2) AIN-vitamin mixture (g / kg diet): Thiamine HCl 0.6 g / kg, Biotin 0.02 g / kg, Riboflavin 0.6 g / kg, Cyanocobalamine 0.001 g / kg, Pyridoxine HCl 0.7 g / kg, retinyl acetate 0.8 g / kg, tycotinic acid 3.0 g / kg, DL-tocopherol 3.8 g / kg, Ca-pantothenate 1.6 g / kg, 7-dehydrocholesterol ) 0.0025 g / kg, folic acid 0.2 g / kg, methionine 0.005 g / kg, sucrose, fine powder to 1,000 g / kg.

3. Weight measurement of adipose tissue

The rats reared in the high fat diet were incised to retroperitoneal adipose tissue, epididymal adipose tissue, and total-abdomonal adipose tissue, and then weighed.

The results are shown in Table 2.

The statistical results of the experimental results were expressed as mean ± standard deviation, and values with the same superscript were not statistically significant by Duncan's multiple rage test (p <0.05).

As shown in Table 2, when 30 mg / kg or 60 mg / kg of raspberry leaf butanol fraction according to the present invention was orally administered to rats fed with a high fat diet, dorsal cavity adipose tissue, epididymal adipose tissue and total abdominal adipose tissue Significantly reduced its weight. On the other hand, in the high fat diet group, the weights of posterior abdominal adipose tissue, epididymal adipose tissue and total abdominal adipose tissue were significantly increased.

4. Serum Lipid Measurement

To determine whether hyperlipidemia was induced in the rats bred with the high fat diet, blood was collected from the orbital vein, and after the experiment was completed, blood was collected from the abdominal aorta of the rat. After collecting blood for 12 hours and coagulating at 37 ° C. for 2 hours, the supernatant was used by centrifuging the serum at 15000 rpm. Serum total lipid was measured using a kit (AM 202-K, Asan) prepared by enzyme method of Richmond et al. Serum phospholipid was measured using a kit (Iatron Chem. Co.) prepared by the enzyme method of Chen et al. Serum triglycerides were measured using a kit (AM 157S-K, Asan) prepared according to McGown et al.

The results are shown in Table 3.

The statistical results of the experimental results were expressed as mean ± standard deviation, and values with the same superscript were not statistically significant by Duncan's multiple rage test (p <0.05).

As shown in Table 3, when 30 mg / kg or 60 mg / kg of the raspberry leaf butanol fraction according to the present invention was orally administered to rats fed with a high fat diet, serum total lipid, phospholipids and triglycerides were significantly reduced. . In contrast, the high fat diet group significantly increased serum total lipid, phospholipids and triglycerides.

5. Serum Cholesterol Measurement

Serum total cholesterol content was measured by Richmon's enzyme method, high density lipid cholesterol (HDL-C) content was measured using a kit (AM 203-K, Asan) prepared by the enzyme method such as Noma. Low-density lipid cholesterol (LDL-C) content was calculated according to Equation 1 according to the method of Fridewald et al.

The results are shown in Table 4.

Low density lipid cholesterol (LDL-C) = total cholesterol-[high density lipid cholesterol (HDL-C) + (triglycerides / 5)]

The statistical results of the experimental results were expressed as mean ± standard deviation, and values with the same superscript were not statistically significant by Duncan's multiple rage test (p <0.05).

As shown in Table 4, when 30 mg / kg or 60 mg / kg of the raspberry leaf butanol fraction according to the present invention was orally administered to rats fed with a high fat diet, serum total cholesterol and low density lipid cholesterol were significantly reduced, High-density lipid cholesterol was significantly increased, and the arterogenic index (AI) was significantly reduced. On the other hand, the high-fat diet group increased total cholesterol and low-density lipid cholesterol, decreased high-density lipid cholesterol, and significantly increased arteriosclerosis risk index.

Examples of preparations for the compositions of the present invention are illustrated below.

Formulation Example 1  : Preparation of Pharmaceutical Formulations

1. Preparation of powder

Raspberry Leaf Extract 2g

1g lactose

The above ingredients were mixed and filled in airtight cloth to prepare a powder.

2. Preparation of Tablets

Raspberry Leaf Extract 100mg

Corn Starch 100mg

Lactose 100mg

2 mg magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

3. Preparation of Capsule

Raspberry Leaf Extract 100mg

Corn Starch 100mg

Lactose 100mg

2 mg magnesium stearate

After mixing the above components, the capsule was prepared by filling in gelatin capsules according to the conventional method for producing a capsule.

Formulation Example 2  : Manufacture of food

1. Preparation of Cooking Seasonings

20 ~ 95% by weight of raspberry leaf extract was prepared for cooking spices for health promotion.

2. Preparation of Tomato Ketchup and Sauce

0.2 ~ 1.0% by weight of raspberry leaf extract was added to tomato ketchup or sauce to prepare tomato ketchup or sauce for health promotion.

3. Manufacturing of Flour Foods

0.5 ~ 5.0% by weight of the raspberry leaf extract was added to the flour, and using this mixture to prepare bread, cakes, cookies, crackers and noodles to produce health foods.

4. Preparation of soups and gravy

0.1 ~ 5.0% by weight of raspberry leaf extract was added to soups and broth to prepare meat products for health promotion, soups and noodles of noodles.

5. Preparation of Ground Beef

10% by weight of raspberry leaf extract was added to ground beef to prepare ground beef.

6. Manufacture of Dairy Products

5-10% by weight of raspberry leaf extract was added to milk, and various milk products such as butter and ice cream were prepared using the milk.

Formulation Example 3  : Preparation of Beverages

1. Preparation of carbonated drinks

5-10% of sugar, 0.05-0.3% citric acid, 0.005-0.02% caramel, 0.1-1% of vitamin C are mixed, and 79-94% purified water is mixed to make syrup, and the syrup is 85-98 Sterilizing at 20 ℃ for 180 seconds to mix with a cooling water 1: 1: ratio of carbon dioxide gas was injected 0.5 ~ 0.82% to prepare a carbonated beverage containing raspberry leaf extract of the present invention.

2. Manufacture of health drinks

Instant sterilization by homogeneously mixing subsidiary ingredients such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%), water (75%) and raspberry leaf extract. , Packed in a small packaging container such as plastic bottles to prepare a healthy beverage.

3. Preparation of Vegetable Juice

5 g of raspberry leaf extract was added to 1,000 ml of tomato or carrot juice to prepare vegetable juice for health promotion.

4. Preparation of Fruit Juice

1 g of raspberry leaf extract was added to 1,000 ml of apple or grape juice to prepare fruit juice for health promotion.

The raspberry leaf extract according to the present invention is capable of removing nonpolar diterpenes, phenolic substances, salts and sugars by passing through a solvent fraction, an activated carbon column and an HP-diaion column, thereby allowing the removal of 19 alpha-hydroxyuric acid-type triterpenes. It is possible to obtain a high-purity raspberry leaf extract containing about 10 species of nooid compounds. In addition, the use of rarely used raspberry leaves allows economical and mass production. In addition, raspberry leaf extract according to the present invention significantly reduces the weight of the adipose tissue, serum lipids, serum total cholesterol and low-density lipid cholesterol of rats bred in a high-fat diet, significantly increases high-density lipid cholesterol, risk of atherosclerosis Significantly decreases the index (AI), which can be useful for inhibiting obesity and preventing or treating hyperlipidemia.

Claims (8)

Obesity inhibition pharmaceutical composition comprising raspberry leaf extract. The pharmaceutical composition for inhibiting obesity according to claim 1, wherein the raspberry leaf extract is a raspberry leaf butanol fraction obtained by sequentially extracting and separating the raspberry leaf methanol extract with hexane / ether / butanol. Pharmaceutical composition for preventing or treating hyperlipidemia comprising raspberry leaf extract. The pharmaceutical composition for preventing or treating hyperlipidemia according to claim 3, wherein the raspberry leaf extract is a raspberry leaf butanol fraction obtained by sequentially extracting and separating the raspberry leaf methanol extract with hexane / ether / butanol. Obesity inhibitory food composition comprising raspberry leaf extract. The food composition for inhibiting obesity according to claim 5, wherein the raspberry leaf extract is a raspberry leaf butanol fraction obtained by sequentially extracting and separating the raspberry leaf methanol extract with hexane / ether / butanol. Useful food composition for the prevention or treatment of hyperlipidemia comprising raspberry leaf extract. The food composition of claim 7, wherein the raspberry leaf extract is a raspberry leaf butanol fraction obtained by sequentially extracting and separating the raspberry leaf methanol extract with hexane / ether / butanol.