KR20080042925A - Agent for control of rice seed-borne disease and method for the control - Google Patents

Abstract

An agent for controlling a rice seed-borne disease comprising, as an active ingredient, a calcinated calcium which has an antagonistic effect on the rice seed-borne disease and is produced by calcination of a seashell; and a method for controlling a rice seed-borne disease in a rice seed, the method comprising the step of contacting the agent with the rice seed.

Description

Agent for control of rice seed-borne disease and method for the control}

The present invention relates to a rice seed disease control agent and a control method.

Currently, plant diseases, such as rice seed disease, Kidari disease (pathogen name: Gibberella fujikuroi), bacterial blight (pathogen name: Burkholderia plantarii), bacterial rice blight (pathogen name: Burkholderia glumae), and rice bacterial streaks ( Pathogen name: Acidovorax avenae subsp.avenae) is a disease that occurs in rice seedling boxes and is a plant disease that causes serious problems in water seedlings. For the purpose of controlling these plant diseases, chemical agents with antibacterial activity such as prodrugs, pepurazoate and ifconazole, and inorganic copper, oxolinic acid, and kasgamycin Prevention is also carried out with a combination of chemical agents with antibacterial activity.

In addition, mineral or alkali earth metal carbonates or hydrogencarbonates, such as calcium carbonate (CaCO ₃ ), which are currently obtained from marble or limestone, are also used as additives in foods, pharmaceuticals, and dietary supplements. As a soil improver for adjusting pH and a calcium carbonate agent for the purpose of alleviating the weakness of inorganic copper, registration as a fertilizer has been widely used in agriculture.

Among the above-mentioned conventional techniques related to the control of rice seed plant diseases, in Patent Literature 1 or Patent Literature 2, carbonates or hydrogen carbonates of alkali metals or alkaline earth metals such as calcium carbonate or hydrogen carbonates, alkali metals or alkaline earth metals It has been proposed to use hydroxides for bacterial rice blight, rice limb disease and bacterial rice blight.

Moreover, as a prior art which controls rice seed by the combination of a calcium carbonate agent and a chemical | medical agent, in patent document 3 and patent document 4, carbonates, such as calcium carbonate, ifconazole, benomil, which are pesticides with antimicrobial activity, It is also proposed to use it as a rice seed disinfectant by combining chemicals, such as prochloraz.

In addition, as a prior art for soil infectious plant diseases, Patent Literature 5, the active lime based on calcium carbonate and a soil fungicide as an active ingredient is effective in controlling soil diseases such as root gall disease, etc. Is disclosed.

On the other hand, the technique which applies the knowledge of the natural system which organically functions the composition, structure, a component, etc. regarding the birth, growth, and extinction of a living body to the design of an artifact has been discovered recently. For example, it is applied to agrochemicals using microorganisms as an active ingredient. However, these microbial pesticides have excellent properties in terms of environment, but many of them are expensive.

Therefore, the technique of applying the organic and inorganic components contained in the animal shellfish to the soil pest control material has recently been devised, and Patent Document 6 discloses that the chitin-containing organic matter contained in the animal shellfish of crab and shrimp is sprayed onto the soil and the like. The present invention discloses a soil disease control method for the purpose of settling actinomycetes producing chitinase separated therefrom in soil.

On the other hand, shells such as scallops, abalones, oysters, and shellfish shells in the same animal shellfish are producing about 1 million tons per year, and many are buried as waste, but they are about 1 million tons per year. The amount of waste is enormous. For this reason, in order to reduce the amount of waste, the effective utilization measures have been considered so far in research institutes and companies including each municipality.

The components of these shells are generally 99% calcium carbonate and 1% organic matter (varies depending on the structure), and has a very high toughness (same as plastic), which is an excellent natural composite material. Shells having a three-layer structure using carbon dioxide dissolved in the sea as a carbon source form calcium carbonate from calcium in the sea, forming a unique structure.

When the shells including scallops are calcined, calcium carbonate, which is a component thereof, is generally converted into calcium oxide (CaO).

Calcined calcium obtained by calcining shells (calcined calcium is calcium oxide (CaO) obtained by slowly decarburizing (removing carbon dioxide) from calcium carbonate, which is a main component, by mainly calcining shells such as scallops or clam shells at a high temperature, or Calcium oxide and calcium carbonate, which are referred to herein as "calcined calcium" in the present specification, are calcined at, for example, 500 to 1200 ° C, so that the calcining of calcium carbonate and organic matter inside the shell, and further decarbonization It proceeds slowly and finally calcined to calcium oxide. When the firing temperature is increased, almost all are converted to calcium oxide. When the firing temperature is low, some are converted to calcium oxide, but the remainder remains as calcium carbonate.

The alkali component of calcium oxide contained in these calcined calcium and the porous structure of the shell itself have been known to increase the disinfecting effect, the deodorizing effect and the antibacterial property.

Among these, application to seed diseases has recently been attracting attention, and in Patent Literature 7, by using a mixture of calcined calcium, percarbonate, and a coating agent, the seed surface is coated by film coating, coating granulation, or the like. A method of controlling from seed diseases such as, cabbage and spinach is disclosed.

In addition, as a method of controlling specific diseases of rice using microbial pesticides without using chemical pesticides, Patent Document 8, Patent Document 9, and Patent Document 10 describe Pseudomonas bacteria in bacterial rice blight, bacterial rice blight, and rice. It is disclosed that it is used for long leg disease and the like.

Patent Document 1: Japanese Patent Application Laid-Open No. 9-271211

Patent Document 2: Japanese Patent Application Laid-Open No. 2004-262926

Patent Document 3: Japanese Patent Application Laid-Open No. 5-221812

Patent Document 4: Japanese Patent Application Laid-Open No. 5-238911

Patent Document 5: Japanese Patent Application Laid-open No. Hei 6-92812

Patent Document 6: Japanese Patent Application Laid-Open No. 7-2614

Patent Document 7: Japanese Patent Application Laid-Open No. 2004-292354

Patent Document 8: Japanese Patent Application Laid-Open No. 11-302120

Patent Document 9: Japanese Patent Application Laid-Open No. 2002-017343

Patent Document 10: Japanese Patent Application Laid-Open No. 2003-261409

Summary of the Invention

As said seed disease control method, the conventionally known control agent in patent document 1 and patent document 2 can supply a large amount inexpensively, and attempts to control a disease using these. However, there is a control effect under the occurrence of small to moderate disease, but it cannot be said that the control value is sufficient under conditions of high disease occurrence, that is, under the occurrence of heart (see Comparative Example 1-3 of the present specification).

In addition, in the method of Patent Document 7, the seed disease can be controlled by coating the seed surface of the crop with a coating material containing calcined calcium. However, as in Patent Documents 1 and 2, the control effect was not so high under seam generation. .

Therefore, the problem of the present invention is for disinfecting rice seeds, which has a high control effect against rice seed disease, and furthermore, there is no environmental pollution such as water pollution, which has been a problem with conventional seed disinfectants, and economically not burdened. Development of the control agent was desired.

MEANS TO SOLVE THE PROBLEM The present inventors earnestly examined in order to solve the said subject. As a result, it was found that calcined calcium obtained by calcining shells including scallops, abalones, oysters, and shellfish clams had a high control effect against rice seed diseases such as dyke leg disease, bacterial rice blight disease, and bacterial rice blight disease.

In this invention, compared with the method of patent document 1 and patent document 2, by using calcined calcium for a rice seed disease, it discovered that it had a remarkably high control effect.

Although the cause is not clear, shells, such as a scallop, abalone, an oyster, and a clam, used by this invention are characterized by organic substance and skeletal structure (multilayer structure, porous) compared with mineral baking. The unique structure and specific function of the multi-layered porous structure of the calcined calcium itself of the shell exhibits high antimicrobial properties, antimicrobial retention properties and long-lasting properties. It is estimated that the effect was obtained.

On the other hand, in patent document 7, shell calcined calcium is used for the disease control of vegetables under specific conditions, and the remarkable control effect is discovered. However, the effect is a medium degree as apparent in the Example of patent document 7, and there existed a problem that the effect especially under shim generation is comparatively small.

On the other hand, in the present invention, by using calcined calcium in the above-mentioned specific rice seed diseases, surprising knowledge is obtained that achieves the same or more control effect (see Examples and Comparative Examples herein).

In addition, as disclosed in Patent Literature 7, it has been used by mixing minerals such as shell components and coating materials, not shell components alone as a control agent for seed diseases. However, the present inventors also obtained the knowledge that a high control effect was obtained even if the calcined calcium of a shell was used alone for rice seeds without using minerals, such as a coating material.

Thus, there is no example in which the shelled product was applied to rice seed disease, and not only seed disease caused by filamentous bacteria such as rice leg disease, but also seed disease caused by bacteria such as bacterial rice blight and bacterial rice blight. On the other hand, the knowledge which has the same or more excellent control effect as the conventional chemical | medical agent, and has a high control effect by using the calcined product of a shell alone for rice seed like this invention until now is unknown. .

According to the present invention, a rice seed disease control agent comprising calcined calcium obtained by firing a shell having an antagonistic ability against rice seed disease as an active ingredient, and contacting rice seed A method of controlling rice seed disease of rice seeds is provided.

details

According to the control agent containing the shell calcined calcium of this invention as an active ingredient, it is possible to control the specific kind of rice pests economically, and to be excellent in the environment, and to control at high control price.

In addition, the use of the control agent of the present invention has no environmental pollution as in the conventional chemicals, and does not cause any damage due to high pH.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail. The present invention provides a rice seed disease control agent containing calcined calcium obtained by firing a shell as an active ingredient having an antagonistic ability against rice seed disease and a method of controlling rice seed disease using the same.

First, the control agent of this invention is described. In the present invention, examples of the rice seed disease of the control agent containing calcined calcium obtained by calcining the shell as an active ingredient include bacterial rice bran disease, bacterial rice bran disease, and rice leg disease.

As the shell used in the present invention, detailed firing conditions will be described later. However, if the shell before firing is a shell containing calcium carbonate as a main component, the shell is not particularly limited, and specifically, shellfish, clam shell, scallops, abalone, oyster, clam shell, Screw clams, cherry clams, turban shells, clams, key clams, slugs, bird clams, clams, improved clams, and the like.

As shells, it is preferable that they can be normally obtained from shellfish farms, processing plants, and the like. Among the shells, shells of shellfish, scallops, abalone, oysters, shellfish clams, cockles, clams, and improved shells are preferably used.

Among them, in view of more easily and in large quantities available, particularly preferably shells of scallops, abalone, oysters, and shellfish clam. Specifically, in Example 1-4, using calcined calcium obtained by baking these using shells of scallops and abalone is one of the preferred embodiments.

These shells may be one type of shells, or two or more types of shells may be mixed. However, it is particularly preferable to use one type of shell because the processing is easy.

The calcined calcium used in the present invention includes calcium oxide (CaO), or calcium oxide and calcium carbonate obtained by slowly decarburizing (removing carbon dioxide) by firing a shell in which the main component before firing is calcium carbonate. . By firing, calcium carbonate, which is the main component (about 99%) before firing, is gradually converted into calcium oxide, and at the same time, firing of about 1% of organic matter contained in the shell before firing also proceeds simultaneously.

There is no particular limitation on the ratio of calcium carbonate (CaCO ₃ ) to calcium oxide (CaO) of calcined calcium. For example, in the range of the component ratio (weight) of calcium carbonate (CaCO ₃ ): calcium oxide (CaO) = 99: 1 to 1:99, control of bacterial rice blight disease, bacterial rice blight disease, and rice leg disease is suitably performed. I can do it.

For example, in the present invention, in the ratio of CaCO ₃ and CaO of calcined calcium, the component ratio (weight) of calcium oxide (CaO) is 96% (trade name CAI-I, manufactured by Nippon Natural Materials Co., Ltd.), calcium oxide ( It is one of the preferred embodiments that the component ratio of CaO) is 18% (trade name CAI-V, manufactured by Nippon Natural Materials, Inc.) and the like.

Further, the firing may be either one type of calcium, It may be that CaCO ₃ and CaO components in the ratio of two kinds or more having different firing calcium are mixed.

In the present invention, calcined calcium is preferably used in a mixed state of calcium carbonate and calcium oxide, but the calcium oxide is hygroscopic, which is partially hydrated to form calcium hydroxide (Ca (OH) ₂ ). There is a case, but performance does not interfere. In view of the above, in the present invention, calcined calcium in which calcium hydroxide components are mixed may be used in addition to calcium carbonate, calcium oxide, and the respective components with respect to the calcined calcium component.

The ratio of calcium carbonate to calcium oxide and each component is different depending on the firing temperature and firing time, and can be appropriately adjusted by those skilled in the art.

There is no restriction | limiting in particular about the calcined calcium used by this invention, What baked and shelled shells, such as a scallop, and made into powder form can be used. Moreover, you may bake after grind | pulverizing.

On the other hand, it is also possible to use what is marketed as a powder product which pulverized calcined calcium, and a person skilled in the art can select suitably.

In addition, when calcined calcium is produced, it is usually calcined at a temperature range of 500 to 1200 ° C. In this temperature range, in this invention, Preferably it is the temperature range of 600-1200 degreeC, More preferably, it is the temperature range of 600-1100 degreeC.

There is no restriction | limiting in particular about baking, For example, general baking apparatuses, such as a rotary kiln and a direct burner, can be used. The firing time varies depending on the firing temperature, but can be appropriately adjusted by those skilled in the art in order to achieve a preferable ratio of the above-described calcined calcium.

The calcined calcium normally uses a pulverized itself. The particle diameter at the time of grinding | pulverization differs with a baking process and a processing method, Although there is no restriction | limiting, Usually, what grind | pulverized to 2-100 micrometers is used, Preferably it is 2-70 micrometers, More preferably, it is 3-45 micrometers. The particle diameter here means the average length (average diameter) of the length of the longest part and the length of the shortest one of the calcined calcium powder. There is no restriction | limiting in particular in the grinding | pulverization method, A general grinding apparatus can be used.

As a specific example of calcined calcium used in the present invention, for example, as shown in Example 1-4, scallop is used as a shell, and the high-temperature calcined product (trade name CAI-I, particle size of 5 µm type and 15 µm) Type, Nippon Natural Material Co., Ltd.) or scallop crushed product (brand name CAI-V, particle size 5 μm type and 15 μm type, manufactured by Nippon Natural Material Co., Ltd.), sintered product of scallop (brand name MC-H) (Magna caps), particle size 5 micrometers type, product made by Asada Shokai Co., Ltd., and other than that, abalone is used as a shell, and a pulverized product (brand name MC-A (magna caps), particle size 5 micrometer type, product made by Asada Shokai Co., Ltd.) There is this. In addition, examples of the same fired product include Kawai Co., Ltd. (trade name "shell calcined calcium H-D"), Step Co., Ltd., and Carflos Corporation (trade name "Shell White").

Next, the control method of the rice seed disease using the control agent of this invention is described. In general, rice seedlings are soaked in water for a certain period of time (inoculation), and the water is sufficiently absorbed by rice seed, and then soaked in water at around 30 ° C for 1 to 2 days, and then treated with malt. And seedling soil, seedling boxes filled with seedling soil, seedlings and the like.

The control agent of the present invention has no problem of weakness and is not limited in use time, and is a rice leg control agent, a bacterial rice bran disease, and a bacterial rice grain control that can be widely used. Although the controlling agent of the present invention may be treated by at least one time in the above-described seedling operation, specifically, the dipping solution of the controlling agent of the present invention is immersed before or after inoculation, during soaking, or during the period of treatment. The wet seed is treated before or after sowing, before the seedling or before sowing, or after the sowing, the dilution of the control agent is used by soil irrigation.

As mentioned above, since it is not necessary to use components, such as a coating | cover material with respect to a seed, in this invention, it is economically advantageous. Moreover, the temperature of the diluting liquid at the time of immersion treatment of rice seed in this control agent dilution liquid at the time of acupuncture or apex is 15 degreeC-35 degreeC, Preferably it is 15 degreeC-30 degreeC. In addition, regarding the processing time, instantaneous processing is performed for 48 hours, preferably 1 hour to 24 hours.

When using the control agent which uses calcined calcium obtained by calcining the shell of this invention as an active ingredient in the said method, for example, in the case of immersion treatment, calcined calcium concentration in immersion liquid is normally 0.01 weight%-1 weight% ( As a dilution factor 10000 times-100 times), Preferably it is 0.1 weight%-1 weight% (1000 times-100 times as dilution times), More preferably, 0.1 weight%-0.8 weight% (1000 times-125 as dilution times) It is preferable to prepare calcined calcium liquid (liquid containing suspended calcium or suspended calcium calcined liquid formed by mixing calcined calcium with water. The same applies hereinafter).

On the other hand, in the case of the treatment of moisture, after removing the wet seedlings treated with invasion or the earliest treatment, the calcined calcium can be mixed with the rice seed, applied and sown. For example, it is good to prepare so that calcined calcium may be 0.1 g-10 g, Preferably it is 0.5 g-5 g with respect to 100 g of rice seeds.

In addition, in the case of soil irrigation, the soil may be treated by infiltrating an aqueous solution such as calcined calcium. For example, 50-500 ml of dilution solution diluted 10-1000 times, preferably 50-500 times, calcined calcium per box of seedling box (size can be appropriately selected, for example 30 × 60 × 3 cm). I am concerned with.

For example, as shown in Example 1-4, after washing water with rice, water is so prepared that the calcined calcium concentration is 0.2% by weight (500 times diluted) or 0.5% by weight (diluted multiple times 200). In order to prepare a calcined calcium liquid, and to perform immersion treatment at 15 ° C. for 24 hours or 48 hours before immersion, or for 24 hours at 30 ° C. for the first time, the immersion treatment is particularly in view of obtaining a high disease suppression effect. It is one of the preferable treatment methods.

Although calcined calcium may be used as it is, it can mix and use with the mineral powder used for a common pesticide. Examples of the mineral powders include talc, calcium carbonate and diatomaceous earth. Although the addition amount of mineral powder is mentioned later, what is necessary is just to determine according to the dilution factor which has a control effect of the part shown in the Example. For example, a calcined calcium having a control effect and a mineral powder can be mixed and used in a water suspension diluted 500 times only of calcined calcium. In this case, for example, if 40 parts by weight of the calcined calcium and 60 parts by weight of the mineral powder are mixed, the resulting mixed powder may be diluted 200 times to prepare an aqueous suspension.

However, in the present invention, the pulverized product of calcined calcium is described above even by carrying out the above-described treatment using a calcined calcium liquid diluted with water without mixing the mineral powder with mineral powder. It does not have to be mixed with one mixed powder to have a particularly high control performance.

For example, in the present invention, as shown in Examples 1-4 to be described later, using a pulverized product of calcined calcium as a liquid diluted with water without mixing with a mineral powder has a high control effect against rice seed disease. In aspect, one of the particularly preferred embodiments.

Moreover, when calcined calcium dissolves in about 0.2 weight% in water, pH will be 12-13, and strong alkalinity will be shown. Compared with quicklime obtained by calcining mineral calcium carbonate, the reactivity is low, but due to the shell's original multilayer porous structure, the retaining power of the alkaline component is increased, so that the effect lasts for a long time. It is also characterized by no weakness due to high pH.

In addition, the control agent in the present invention can be expected to have a control effect against both seed diseases caused by filamentous bacteria such as dyke leg disease and seed diseases caused by bacteria such as bacterial rice blight and bacterial rice blight.

Moreover, since it does not cause the fall of the control effect by the appearance of the pesticide resistant bacteria shown in the conventional chemical | medical agent, it is very useful.

In addition, calcined calcium is added to food as its own calcium source, or sprayed with several wt% aqueous solution of calcined calcium, and used for the control of pathogens, bacteria, molds, and the like, or as an aqueous solution in household use. It is also used to wash residual pesticides in vegetables and fruit trees. Moreover, it is used also as an addition of wall materials, such as plaster, for building use. It is also applied to pharmaceuticals. Calcined calcium is not restricted by the Joint Food Additives Experts (JECFA) of the United Nations Food and Agriculture Organization (FAO) and the World Health Organization (WHO). There is no problem with safety.

Although an Example is shown next, this invention is not limited by the following Example.

Example 1

Inhibitory Effect of Shelled Calcium on Bacterial Blight Blight

Crushed product (made by Nippon Natural Materials Co., Ltd., brand name CAI-I, particle size 5 micrometers type) of scallop, crushed product (brand name MC-H (magna caps), particle size 5 micrometer type, product made by Asada Shokai Co., Ltd.) of scallops ), The pulverized product of the abalone fired product (brand name MC-A (magnacaps), particle size 5 μm type, manufactured by Asada Shokai Co., Ltd.) was diluted 200 times and 500 times with water, and the calcined calcium concentrations were 0.5 wt% and 0.2 wt%, respectively. The calcined calcium liquid was prepared so as to be. Bacterial rice blight contaminated rice (kind: Koshihikari) was put into this liquid, and the immersion treatment was performed at 15 degreeC for 24 hours before immersion. Thereafter, the seedlings were seeded after invasion and malaria according to a conventional method, and seedlings were carried out for 2 weeks in a glass greenhouse.

The results are shown in Table 1, Table 2, and Table 3. Bacterial rice blight assay evaluated and evaluated the incidence from the incidence of seedlings.

Onset index 5: death, 3: onset, less than half of height grown healthy, 1: outbreak, overgrown height of half healthy, 0: sound

Incidence = 100 × {∑ (value of exponent) × (number of individuals corresponding to each exponent)} ÷ {5 × (number of survey seedlings)}

Control value = 100 × {(degree of disease in untreated plot)-(degree of disease in untreated plot)} ÷ {degree of disease in untreated plot)

Comparative Example 1 Inhibitory Effect of Quicklime (CaO) on Bacterial Blight Blight

The quicklime immersion treatment was performed in the same manner as in Example 1 except that the quicklime (CaO) was diluted 200 times and 500 times with water, and a liquid was prepared so as to be 0.5% by weight and 0.2% by weight.

 The results are shown in Table 4. Assay of bacterial rice bran disease calculated and evaluated the incidence from the incidence of seedlings. In addition, the calculation method of the incidence degree and the control value was performed similarly to Example 1.

As shown in Table 4, in the case of quicklime (CaO), it turns out that the control effect is low compared with the calcined calcium process tool of Example 1.

Reference Example 1 Inhibitory Effect of Pseudomonas sp CAB-02 (Pseudomonas sp.Microorganism Accession No. FERM P-15237) on Bacterial Rice Bran Disease

As a reference example, Pseudomonas sp. CAB-02 (Pseudomonas sp.Microorganism Accession No. FERM P-15237) was used, and the preparation containing 1 × 10 ¹⁰ cfu / g of CAB-02 strain was diluted 200-fold with water, and the bacterial concentration was 5. Prepared to be 10 ⁷ cfu / ml. Subsequently, bacterial paddy soil contaminated rice (kind: Koshihikari) was placed in the obtained suspension, and the immersion treatment was performed at 15 ° C. for 24 hours before immersion. Thereafter, the seedlings were seeded after invasion and abduction according to a conventional method, and seedlings were carried out for 2 weeks in a glass greenhouse.

The results are shown in Table 5. Assay of bacterial rice bran disease calculated and evaluated the incidence from the incidence of seedlings. In addition, the calculation method of the incidence degree and the control value was performed similarly to Example 1.

As shown in Table 5, when CAB-02 was used, it was found that the control effect was lower than that of the calcined calcium treated tool under the same disease-generating condition as in Example 1.

Example 2

Inhibitory Effect of Shell Calcium on Bacterial Rice Blight

Calcined calcium (manufactured by Nippon Natural Materials, Inc., trade name CAI-I, particle size of 5 µm) was diluted 200 times and 500 times with water to prepare a liquid such that calcined calcium concentrations were 0.5% by weight and 0.2% by weight, respectively. Bacterial rice blight contaminated rice (kind: Koshihikari) was put into this liquid, and the immersion treatment was performed at 15 degreeC for 24 hours before immersion. Thereafter, the seedlings were seeded after invasion and abduction according to a conventional method, and seedlings were carried out for 2 weeks in a glass greenhouse.

The results are shown in Table 6 and Table 7. Bacterial rice blight assay evaluated and evaluated the incidence from the incidence of seedlings. In addition, the calculation method of the incidence degree and the control value was performed similarly to Example 1.

As shown in Table 6 and Table 7, almost no onset was confirmed in any calcined calcium treatment group regardless of dilution factor, and a high onset suppression effect was recognized.

Comparative Example 2 Inhibitory Effect of Quicklime (CaO) on Bacterial Rice Blight

The quicklime immersion treatment was carried out in the same manner as in Example 2 except that the quicklime (CaO) was diluted 200 times and 500 times with water, and a liquid was prepared so as to be 0.5% by weight and 0.2% by weight, respectively.

 The results are shown in Table 8. Bacterial rice blight assay evaluated and evaluated the incidence from the incidence of seedlings. In addition, the calculation method of the incidence degree and the control value was performed similarly to Example 1.

As shown in Table 8, in the case of quicklime (CaO), it turns out that the control effect is low compared with the calcined calcium process tool of Example 2.

Reference Example 2 Inhibitory Effect of Pseudomonas sp CAB-02 (Pseudomonas sp.Microorganism Accession No. FERM P-15237) on Bacterial Rice Blight

As a reference example, Pseudomonas sp. CAB-02 (Pseudomonas sp.Microorganism Accession No. FERM P-15237) was used, and the preparation containing 1 × 10 ¹⁰ cfu / g of CAB-02 strain was diluted 200-fold with water, and the bacterial concentration was 5. Prepared to be 10 ⁷ cfu / ml. Subsequently, bacterial paddy grain contaminated rice (kind: Koshihikari) was put into the obtained suspension, and the immersion treatment was performed at 15 ° C. for 24 hours before inoculation. Thereafter, the seedlings were seeded after invasion and abduction according to a conventional method, and seedlings were carried out for 2 weeks in a glass greenhouse.

The results are shown in Table 9. Bacterial rice blight assay evaluated and evaluated the incidence from the incidence of seedlings. In addition, the calculation method of the incidence degree and the control value was performed similarly to Example 1.

As shown in Table 9, when CAB-02 was used, it was found that the control effect was low as compared with the calcined calcium treated tool of Example 2 in the same manner as in Comparative Example 2.

Example 3

Inhibitory Effect of Shelled Calcium on Dyeing Leg Disease

Crushed product (made by Nippon Natural Materials Co., Ltd., brand name CAI-I, particle size 5 micrometers type) of scallop, crushed product (brand name MC-H (magna caps), particle size 5 micrometer type, product made by Asada Shokai Co., Ltd.) of scallops ), A pulverized product of the abalone fired product (brand name MC-A (magna caps), particle size 5 μm type, manufactured by Asada Shokai Co., Ltd.) was diluted 200 times, 500 times, 1000 times with water, and the calcined calcium concentration was 0.5% by weight, The liquid was prepared so as to be 0.2 weight% and 0.1 weight%. A rice paddy contaminated rice (variety; Koshihikari) was put into this liquid, and immersion treatment was performed at 15 ° C for 24 hours or 48 hours before immersion. Thereafter, the seedlings were seeded after invasion and abduction according to a conventional method, and seedlings were performed for two weeks in a glass greenhouse.

The results are shown in Tables 10-14. Assay of calf leg disease of calcined calcium calculated and evaluated the incidence rate and control value from the incidence of seedlings.

Incidence rate = painting rate + corruption rate

Control value = 100 × {(occurrence rate in untreated)-(occurrence rate in untreated)} ÷ {occurrence rate in untreated

Painting rate = 100 × {(painted seedlings) ÷ (total seedlings)}

Corruption Sapling Rate = 100 × {(Number of Corrupt Deaths ÷ (Total Number of Deaths)}

In any of the calcined calcium treated groups of Tables 10-14, regardless of the degree of disease occurrence conditions, dilution factor, and immersion time, there was almost no onset, and a high inhibitory effect could be confirmed.

Comparative Example 3 Inhibitory Effect of Quicklime (CaO) on Dyeing Leg Disease

The quick dipping treatment was performed in the same manner as in Example 3, except that quicklime (CaO) was diluted 200 times with water and a liquid was prepared so as to be 0.5% by weight.

 The results are shown in Table 15 and Table 16. Assay of fumigation leg disease calculated and evaluated the incidence rate and control value from the incidence of seedlings. In addition, the calculation method of the incidence seedling ratio and the control value was performed similarly to Example 3.

As shown in Table 15 and Table 16, in the case of quicklime (CaO), it can be seen that the control effect is lower than in the calcined calcium treated tool under the same disease-generating condition as in Example 3.

Example 4

Inhibitory Effect of Shelled Calcium on Dyeing Leg Disease

The pulverized product of the scallop high temperature calcined product (made by Nippon Natural Materials Co., Ltd., product name CAI-I, particle size of 5 μm) is diluted 200 times and 500 times with water, and the liquid is so prepared that the calcined calcium concentration is 0.5% by weight and 0.2% by weight, respectively. It prepared. The rice paddy contaminated rice (variety; Koshihikari) was put into this liquid, and the immersion treatment was performed at 30 degreeC for 24 hours. Thereafter, the seedlings were seeded after invasion and abduction according to a conventional method, and seedlings were carried out for 2 weeks in a glass greenhouse.

The results are shown in Table 17. Assay of calf leg disease of calcined calcium calculated and evaluated the incidence rate from the incidence of seedling. In addition, the calculation method of the incidence seedling ratio and the control value was performed similarly to Example 3.

As shown in Table 17, also in the most immersion treatment, a high onset inhibitory effect was recognized regardless of the dilution factor.

Claims (8)

A rice seed disease control agent comprising calcined calcium obtained by firing a shell having an antagonistic ability against rice seed disease as an active ingredient. The control agent according to claim 1, wherein the rice seed disease is rice leg disease, bacterial rice blight disease, and bacterial rice blight disease. The control agent according to claim 1 or 2, wherein the type of shell is scallops, abalones, oysters, and shellfish. The rice seed disease control method of the rice seed characterized by contacting the seed control of any one of Claims 1-3 with rice seed. A control method for rice seed disease of a rice seed, characterized in that the control agent of any one of claims 1 to 3 is mixed with water to form a calcined calcium solution and rice seed is immersed. The method according to claim 5, wherein the immersion treatment is performed by dipping rice seed before, during or after immersion in calcined calcium liquid. The method according to claim 5 or 6, wherein the calcined calcium liquid is soaked in instantaneous time to 48 hours and rice seed. The method according to any one of claims 5 to 7, wherein the calcined calcium liquid is prepared by preparing the calcined calcium concentration so as to be 0.01% by weight to 1% by weight.